Respiration of Germinating Seeds B-11
Contents
1. than in the non germinating beans measurement Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 12 NeuLog EJ Summary questions 1 What was the ratio between respiration rates of the germinating beans and the non germinating beans in your experiment Why is there such a difference How did the ice cold water affect the respiration rate of the germinating beans Explain What other condition change besides cold temperature could be applied to the germinating beans in this experiment How would this change in conditions affect the respiration rates Draw a flow chart that describes the life cycle of a plant Experiment B 11 Respiration of Germinating Seeds Ver 3 2 12. the screen and then click on the Linear fit button NeuLog lt 1 Gl 9 tb ce m ey gt Back Expenment Load top Sav Open Setup anual a O eae eel Suncweandt CO gt id 1 Undo all functions Back Linear CO p 345 2 2 5 3 3 5 45 Q Ha lo is iar ES BBL Be F one i unctions Grid Graph Table Point Area This is the linear fit function we received Y 0 015X 355 The respiration rate is 0 015 ppm minute for the sample experiment Fill in your results in the following table eres beans ppm minute Cane experiment sample experiment Non germinating Room temperature 2 Room temperature o water 29 26 af Click on the Erase Graph icon id Remove the non germinating beans from the bottle Take the tissue paper and dry 30 of the germinating beans Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 9 NeuLog EJ 28 Put them inside the CO sensor bottle 29 Close the bottle using the COs probe and wait for three minutes before starting the measurement 30 Click on the Run Experiment icon CJ to start the measurement 31 Click on the Zoom fit icon gs during the measurement to see better how the graph changes 32 Your graph should be similar to the following NeuLog D G p E gt oe Back Expenment Erase Single Run Setup Experiment Step Experiment Experiment Experiment a 1 5 Qabe ee BB BBL ke Unzoom Zoom Zoom Select Line Show Grid Graph Table Poin
3. 1 NeuLog EJ Experiment B 11 Respiration of Germinating Seeds 4 Aa N Objectives To learn about cellular respiration dormancy and germination To compare respiration rates of germinating and non germinating beans To investigate the effect of ice cold water on the respiration of the germinating beans Modules and Sensors PC NeuLog software USB 200 module F2 NUL 220 CO logger sensor BAT 200 Battery module optional Equipment and Accessories Glass bottle for the CO2 sensor included with the sensor Plastic container 250 ml beaker The Plastic container and beaker are included in the NeuLog Utility accessories UTL KIT Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 2 NeuLog EJ Materials Tissue paper Disposable spoon 90 beans 200 mi of ice cold water Introduction In cellular respiration chemical energy stored in carbohydrates turns into energy that can be used by the cell in the form of ATP adenosine triphosphate Cellular respiration with or without the presence of oxygen occurs in all living cells including plants This is the chemical reaction for cellular respiration with glucose C Hi206 60 gt 6CO 6H O energy ATPs heat Many plants generate seeds to produce their offspring After a seed drops from a plant it usually goes into a resting period called dormancy which can sometimes take a few years In this period the seed respires very slow
4. g the sensors offset button continuously 3 5 seconds If needed disconnect the sensor from the USB 200 module and quickly connect it to a battery module Come back inside and make sure again that the CO values are relatively stable they should be higher than outside Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 6 NeuLog EJ 14 Close the bottle with the non germinating beans using the CO probe and wait for three minutes before starting the measurement Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 7 NeuLog ix 15 Click on the Run Experiment icon C to start the measurement 16 Click on the Zoom fit icon gs during the measurement to see better how the graph changes 17 Your graph should be similar to the following NeuLog Al EA 9 its gt E o gt Back Expenment Load Erase un Stop Sav Open Setup Experiment Experiment Experiment Experiment Experiment Experiment a dmg k ee Unzoom Zoom Zoom Select Line Show Grid Graph Table Point Area All Fit Zone Width Functions 18 Save your graph 19 You can see that there is a slight increase in the CO level This indicates a very small respiration rate as expected 20 Click on the Select zone icon le and select the linear area of the graph 21 Click on the Show Functions icon s Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 22 23 24 8 NeuLog EJ Click on the CO button on the left of
5. ly and does not germinate This is the reason why nuts have so many calories in them they use them to Survive during dormancy There are different cues needed to break dormancy in different species deterioration of the seed coat elevated soil nutrients soil temperature rain light etc After emerging from dormancy the seed is able to germinate Germination requires high energy to occur therefore cellular respiration increases as a seed emerges from dormancy In this activity we will measure the respiration rates of non germinating beans and germinating beans by using a COs logger sensor and compare the results We will also measure the respiration rate while the germinating beans are cooled by ice cold water Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 3 NeuLog EJ Procedure Experiment setup Prior preparation 1 Set up the experiment as shown in the picture below 2 Put 60 beans inside the plastic container and add some water The water should almost cover the beans 3 Prepare 30 more beans in the CO sensor glass bottle and leave it open 4 Leave the beans in this way overnight or a few hours before the experiment Additional preparation 5 Make sure you have some tissue papers a plastic spoon and ice cold water in a 250 ml beaker Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 4 NeuLog EJ sensor setup 6 Connect the USB 200 module va to the PC 7 Check that the CO se
6. nsor is connected to the USB 200 module Important You must wait at least 30 minutes after connecting the sensor to the USB 200 module or to the battery module before beginning offsetting and measurements Note The following software functions are explained in short It is recommended to practice the NeuLog application functions as described in the user manual beforehand 8 Run the NeuLog application and check that the COs sensor is identified Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 5 NeuLog EJ settings 10 Click on the On line Experiment icon to in the NeuLog main icon bar Click on the Experiment Setup icon Ey and set the Experiment duration to 5 minutes Sampling rate to 60 per minute Testing and measurements 11 12 13 When offsetting the sensor the value is set to 380 ppm which is the outdoor air value of COs in most locations It is recommended to offset the sensor outside you can get it out through a window to avoid a shift in the values if you choose to offset the sensor indoors the calculated rates will still be correct After the sensor was connected to the USB 200 module or to a battery module for at least 30 minutes check that the values are relatively stable remember that your breathing affects the values If the values are not stable keep the sensor connected some more time and check again Go outside wait a few minutes and offset the sensor pressin
7. t Area All Fit Zone Width Functions 33 Save your graph 34 You can see that there is a larger increase in the COs level in the germinating beans compared to the non germinating beans Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 10 NeuLog x 35 Click on the Select zone icon le and select the linear area of the graph 36 Click on the Show Functions icon ie 37 Click on the CO button on the left of the screen and then click on the Linear fit button NeuLog lt 1 EA rp e D coe Back Expenment Erase Single Run Save Open Setup Expenment Step Expenment Expenment Expenment CO gt id 1 Undo all functions Back Linear CO 1 5 R RIA le B43 Time Minute 3 IA Unzoom Zoom Zoom Select Line Show Grid Graph Table Point Area All Fit Zone Width Functions 38 This is the linear fit function we received Y 1 256X 663 The respiration rate is 1 256 ppm minute for the sample experiment 39 Fill in your results in the previous table Experiment B 11 Respiration of Germinating Seeds Ver 3 2 1 11 NeuLog EZ Challenge research 40 _ Fill the plastic container with 200 ml of ice cold water 41 Insert the bottle with fresh germinating beans dried with tissue paper Wait for 5 minutes and repeat the experiment 42 What results would you expect to see Will the rate be higher or lower than in the germinating beans at room temperature measurement Will the rate be higher or lower
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