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1. 954 957 968 969 970 Press Save to enter file save interface Press Enter to check detailed gradient distributing situation Please refer to the following 1 For the new edited PCR program please save first then run it otherwise the file cannot be run 2 When setting Time as the hot lid will be closed automatically when the block reaches the target temperature BOECO TC PRO Thermal Cycler In this interface press Right Left to enter the file name Press Enter to move the cursor forwards one position Select User File Name and Password through pop up key Click left button to delete characters while clicking right button to move the cursor Press Caps to shift the capital or small letter and there will be a capital or small letter indication on the left top of the screen as the example a8 Chapter Four Operation Guide File Save Interface Hotlid off 2007 11 1 10 05 00 abc BOCEO TKK KK User File Name Password Password Check lt Press Main to go back to the main interface Caps Save Press Save to save the file When the file is normally saved the system will remind File have been saved Please continue another operation Press Enter to cancel the reminding bar If the file was used the system will remind you to rename Rename or conti
2. The user name can contain only letters and numbers Y OK X Cancel Copy user 1 Choice the user name needed to copy 2 Choice the Copy User menu under User menu Paste user 1 Choose the name of the equipment list or any user name 2 Choice the Paste User menu under User menu Clew 1 If the copy user already occurs and the file is opening can t been plaster user 2 Only can plaster between the local computer and equipment BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Delete user 1 Choice the user need to be deleted 2 Choice the delete User menu under User menu Clew Can t delete user when the file open 1 Choice the local computer 2 Choice the Import User menu under the User menu 3 Choice the imput list click the open button Clew Can t imput user if the existent user is opening 1 Choice the user which want to export in the local computer 2 Choice Export User menu under User menu 3 Choice the export list click ok button Clew Can t export user when the file open 9 File menu How 3 Create new file 3 Open and edit file 3 Copy file 3 Paste file BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 2 Delete file 2 Change file name 2 Change the file password 2 Save file 3 imput file 3 Export file Create new file 1 Choice the user which need to found new file 2 Choice the Create Ne
3. Input target equipment Port Monitor P Address Report Output target equipment ES 2008 1 3 14 26 33 2008 1 314 35 18 2008 1 3 14 35 39 2008 1 3 14 35 49 2008 1 3 14 Found Network Enabler Module s 1 Configuration Changed NE 41105 00 90 Configuration Changed NE 41105 00 30 E8 10E287 Ny Network Enabler Administrator COM Mapping File Function COM Mapping View Help DOA SA Function Network Enabler Admir Configuration i COM Mapping Monitor Port Monitor IP Address Report COM Mapping 1 COM 2008 1 3 14 26 33 2008 1 3 14 35 18 2008 1 3 14 35 39 2008 1 3 14 35 49 2008 1 3 14 37 22 Found Network Enabler Module s 1 Configuration Changed NE 4110S 00 90 E8 10 E2 B7 Configuration Changed NE 41105 00 0 E287 Configuration Changed NE 4110S 00 90 8 10 E2 B7 Configuration Changed NE 41105 00 90 E8 10 E2 B7 Double click the searched IP address to set the mapping property page choice Advanced Setting as below CON Port Settings Port Number 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Tx Mode Classical FIFO Enable Fast Flush Only Flush Local Buffer Apply All Selected Ports OK X Cancel Choice Serial Parameters as below CON Port Settings Port Number 1 Part s Selected 1st port is Port 1
4. Edit to edit the file indicated by the cursor Delete lt gt Press New File to edit a new file Press Delete and the system will alarm Confirm delete file then according to the alert to select Delete or BACK Ifthe current file doesn t have code press Run to enter into File Running Interface and tube type confirmation If the current file has code press Run thus there appears a dialog box Input Password lt Ifthe module is mixed block determine the test tube type first pop up the following dialog BOECO TC PRO Thermal Cycler Chapter Four Operation Guide box Select Tube Type 02m If the current Control Mode is block mode press Run running into the file operation interface If the current control mode is Tube mode press Run key then pop up the following dialog box Select Sample Volume 0501 I The same as main interface user could input the actual password sample type and number of sample volume Unit Micro liter according to the practicality Note The sign at the top of User and File indicates the current running file is default one which can be modified in the parameter setting interface File Edit Interface In this interface you can edit sections and segments of the file A File is comprised by sections and segments And the sections contain many segments which you can set cyclers to be r
5. x10 From 01 to 03 segment you can set setting B Block x well temperature Temp duration Time Default User BOECO Default File PCR2 Ramping rate Ramp temperature Control Mode Block increment of every cycler Temp and time increment of every cycler Time Press Delete to delete the present segment Press Cycle to enter into Section Edit Interface where you can set cycle numbers and Cycle Delete initial cycle segment The figure shows a cycle from the first segment to the third one and there are totally 10 times cycles Press Cycle repeatedly to add sections max 5 sections Press Delete to delete the present segment Move the cursor by pressing Up Down to enter the former segment or the latter Press Save to enter file save interface Press Back to go back to the File List Interface Special Warning BOECO TC PRO Thermal Cycler Chapter Four Operation Guide 1 For the new edited PCR program please save first then run it otherwise the file cannot be run 2 When setting Time as the hot lid will be closed automatically when the block reaches the target temperature File Save Interface In this interface press Digit amp Letter to BOECO Version 2007 1 0 A 2007 11 1 10 05 00 enter the file name Press Enter to move the cursor forwards one position Select User File Name and Pass
6. Basic Settings Advanced Settings Serial Parameters Baud Rate Parity None Data Bits 8 Stop Bits 1 Flow Control None Apply All Selected Ports w OK X Cancel Click ok button application configuration 4 Run upgrade exe 5 Press open key choice the upgrade procedure in the computer 6 Press open key the computer start to upgrade the software of instrument 7 Software under upgrade the apparatus will clue on Configuring Mode see 8 After the software upgrade finish the apparatus will clue on Upgrade finished see 9 Firmware upgrade finished 10 Click remove equipment to remove the equipment mapping 11 Resume configuration Network Enable Adminastrator Choice configuration function N Network Enabler Administrator Configuration File Function Configuration View Help 222 LON Function Configuration 0 Network Enabler Module s BY Network Enabler Admi No Model MAC Address IP Address Status Configuration d COM Mapping Monitor configuration dai gt Message Log 0 Monitor Log 0 No Time Description 0 0 0 0 0 0 0 0 O Now 2008 1 3 14 15 25 BOECO TC PRO Thermal Cycler Chapter Six Firmware Upgrade Double click the searched IP address open the property page to choice the network option Configuration Information Model NE 41105 Basic Network Serial Iv Modify MAC Address 00
7. Choose the user option under the equipment list 2 Choice the Paste File menu under File menu Clew Only can paste file between computer and equipment Can t cover the opened existent file BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Delete File 1 Choice the file need to delete 2 Choice the Delete File menu under the File menu Clew Can t delete the opened file Change the file name 1 Choice the file which user want to change the name 2 Choice the Change File Name menu under File menu 3 Input file name click OK button Clew Can t change the opened file name Change File Name Do you want to change file name of Computer gt TTTT gt 1 Enter new file name The file name can contain only letters and numbers Y OK X Cancel Change the file password 1 Choice the file which want to change the password 2 Choice the Change File Password menu under File menu pop up dialog box 3 Input the old password the new password and click OK button Clew Can t change the opened file name BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Change File Password Do you want to change password of Computer gt TTTT gt 1 Enter old password Enter new password The password can contain only numbers Enter new password again Use empty password Y OK x Cancel Clich Save File option to save the file after modifi
8. 00 0 Total Time 00h 56m Now Running Time 00 00 Cyclel 01 of 10 Remainig Time 00h 56m Stop Start Skip View File seconds the system will continue running automatically Press View File to view edited PCR programs Press Skip to stop current temperature segment and run the next 2 2 How to set system parameters In the main interface press System to enter into system parameter setting interface Press Enter to move the cursor to the next item The option is followed by the symbol N Press Up Down to roll the option for selecting and other information can be input by pressing Digit amp Letter Press Right Left to cursor s position Press Back to go back to the main move menu Press Save to save parameters System Parameter Setting Interface Hotlid off 2007 11 1 10 05 00 Date Time Default User Default File N BOECO Y Block N Hotlid N Key Sound N Run End Sound N Control Mode Back Special Reminding When set the parameter please press the Save otherwise the parameter remains the original one 2 2 1 Date Time state the default system time after power on 2 2 2 Default File Default User state the default running program and the default user name BOECO TC PRO Thermal Cycler Chapter Four Operation Guide 2
9. 3048 30 X 0 5ml 48 X 0 2ml Standard Centrifuge Tube 0 2ml Standard Centrifuge Tube 0 5ml BLOCK 384G 384 well plate Gradient block 384 well plate BLOCK 48D 48 X0 2ml 48 X 0 2ml BOECO TC PRO Thermal Cycler Chapter One Introduction Standard Centrifuge Tube 0 2m1X 2 BLOCK 41 4pcs In Situ plate In Situ plate BOECO TC PRO Thermal Cycler Chapter Two Specifications Chapter Two Specifications This chapter describes the Thermal Cycler s operation transportation and storage conditions basic parameters performance and functions 1 Normal Working Conditions Ambient Temp 10 C 30 C Relative Humidity lt 70 Power Supply 100 240V 600W 50 60Hz Note Before power on please check whether the above working conditions are satisfied Pay special attention to the power line s reliable grounding 2 Conditions for Transportation and Storage Ambient Temp 20 C 55 C Relative Humidity lt 80 3 Basic Parameters Model TC PRO 96G TC PRO 3048 Tse TC PRO 48D nm a Model of Main Body e Model of B 96G B 3048 B 384G B 48D B 41 Block 96x0 2ml 96micro plate 48x0 2ml 30x0 5 Apes of me Er ml 384 well Plate 48 0 2m1 48x0 2 n Situ pacity 8 Strip tube plate 8x12strip tube Temp 4 C 99 C Range Heating gt 4 0 C gt 2 8 C 22 80 24 00 21 80 Rate max Cooling gt 4 0C gt 2 8 C 22 8 C 24 0 C 21 8 C Rate max BOECO TC PRO The
10. ECO default user control mode and sample Hotlid 0ff 2007 11 1 10 05 00 Boeckel Co GmbH Co KG volume are displayed Version 2007 1 0 cay cis Block Grad Press File to enter into the File List Default User BOECO Interface Default File PCR1 Press System to enter into the System ohio MOS Bk Parameter Setting Interface If the current file doesn t have code pm press Run to enter into File Running Interface and tube type confirmation If the current file has code press Run thus there appears a dialog box Input Password The same as single module Press Back to go back to the main interface Press Accept key to confirm the code lt f the current Control Mode is block mode press Run running into the file operation interface If the current control mode is Tube mode press Run key then pop up tube vol Settings dialog box Select Sample Volume 05011 BOECO TC PRO Thermal Cycler Chapter Four Operation Guide 4 1 How to edit a PCR program File List Interface Press File on the Main Interface to enter into the File List Interf th 3 fil Hotlid 0ff 2007 11 1 10 05 00 T S EEE b o SC j tee No User File Name Save Time listed in the form as showed in the picture BOECO1 PCR1 2007 11 1 When the flashing bar is flashing in the User 2 BOECO2 PCR2 2007 11 1 Column select users through pop up key at 3 BOECO3 PCR3 2
11. End Sound stand for the sound of pressing key and reminding sound after the procedure runs over respectively See details in the APPENDIX 2 BOECO TC PRO Thermal Cycler Chapter Four Operation Guide NOTE 1 After set the parameters please press Save to confirm the setting otherwise the parameter remains the original one 2 Before the instrument leaves the factory its inside clock has been adjusted in accordance with Beijing Time 3 For the first use of the instrument you are advised to confirm whether the date and clock time are set right as local time 3 How to run a double block PCR program Switch the power on BOECO TC PRO Thermal Cycler will beep twice and it shows the power supply is connected well The screen will display Self testing and the instrument will carry on self testing The self testing needs about 1 2 minutes and please wait patiently If the self testing is successful the screen will display the main interface Dual block means two blocks Block A and B respectively represent actual block on the left and right Press Shift to operate A or B blocks in the same interface The following introduction takes the example of operating A block The Main Interface In the top center of the main interface BOECO Version 2007 1 0 2007 11 1 it displays the current operating block 10 05 00 As in the illustration it shows the A Block x well control symbol is A Press Shift to Def
12. File List Interface Special Warning 1 For the new edited PCR program please save first and then run it otherwise the file file can t be run 2 When setting Time as the hot lid will be closed automatically in the case that the block controls the temperature Relation of sections and segments in a Section 1 Cyclel X From to Section 2 Cycle2 X From to Seg 16 Section 5 Cycles X From to BOECO TC PRO Thermal Cycler In this interface press Digit amp Letter to input the file name Press enter to move the cursor forwards one position Select User and File Name through pop up key Click left button to delete characters while clicking right button to move the cursor Press Caps key to shift the capital or small letter and there will be a capital or small letter indication on the left top of the screen As H showed in the above picture Press Main to go back to the main interface Chapter Four Operation Guide File Save Interface Hotlid off 2007 11 1 10 05 00 User File Name Password KKK Password Check KKK Caps Press Save to save the file When the file is normally saved the system will remind File have been saved Please continue another operation Press Enter key to cancel the reminding bar If the file was used the system will remind you to rename Rename or cont
13. Four Operation Guide Chapter Four Operation Guide There are five kinds of block units suitable to BOECO TC PRO Thermal Cycler In this chapter we will explain in details on operations of every kind of block unit for example how to edit access modify delete or run a PCR amplification file how to set parameters under different block unit Warning Please turn off the power immediately and contact the distributor if abnormal sound or display appears after power on or if failure alarm and display are found in the process of the device s self testing Note If the number of samples is less than the number of conical holes of the block the sample tubes should be evenly distributed over the block in order to ensure that the heated lid will stably press on the top of the sample tubes so that both the block s load and the temperature distribution in each tube are even Note Before closing the cover please revolve the knob counter clockwise according to the knob diagram till there sound dang dang Please note that the knob can be tightened by revolving clockwise but can be loosed by revolving counter clockwise After closing the cover but before power on please revolve the knob clockwise till it sound dang dang Revolve the knob one more turn At the beginning of revolving the knob clockwise or counter clockwise you can hear dang dang sound press the unlocking device by your finger meanwhile revolve the knob cou
14. Y amp 314 19 1355 QM H Q Cop B Copy user Save file Equipment setup Input user y py Paste file Setup user Delet user Edit file Disconnect link Change file name 7 Basic manipulation Open the file of the equipment or the machine Click Run button Select test tube type and size of the test tube Click ok button operation B QA NA Clew If the file is saved in the computer pre operational options related equipment operation Checking the operating file 1 Document if the equipment running the device will be displayed from the bottom Run File A B Options 2 Double click Run File A B display the operation file content and equipment running states Clew By clicking Pause Stop button to pause equipment running BOECO TC PRO Thermal Cycler TC PRO User File Tools Help Chapter Five Connection Computer 22952832 aa 3 Computer Run File Tenp Time Cycle Times eq106 5 eq104 96G s gt Cuvette volume 60ul Cuvette type 0 5ml Total time 04h 21m Remaining time 04h 21m Hotlid temp 20 50 Module temp 20 70 State Running Check history information Double click the historical option in the System option to show the historical information TC PRO User File Tools Help 18889282 L3 L3 L3 L2 c 8 2 La l 6 8 5 amp os E Ed Com
15. can select START to continue If you don t make a decision within 100 seconds the system will continue running automatically Press View File to view edited PCR programs Press Skip to stop current temperature and run the next Press Enter key checking the grads temperature value lt gt 4 2 How to set system parameters System Parameter Setting Interface In the main interface press System to enter Hotlid 0ff 2007 11 1 system parameter setting interface 10 05 00 Press Enter to move the cursor to next item Date 03 10 22 The option is followed by the symbol N Press Time 14 53 19 N Up Down to roll the option for selecting and Default User BORCOM other information can be entered information by Default File pressing Digit amp Letter Press Right Left Control Mode N to move the cursor s position Hotlid N Press Back to go back to the main Key Sound N EE Run End Sound NGN Press Save to save the parameters Special Reminding When set the parameter please press the Save otherwise the parameter remains the original one NOTE After set the parameter please press Save to confirm setting otherwise the parameter remains the original one Before the instrument leaves the factory its inside clock has been adjusted in accordance with Beijing Time For the first use of the instrument you are advised to confir
16. reaching target temperature namely entering into the Block thermostatical state the time for reagents temperature under target temperature is less than setting time see Fig 1 12 Control Mode Control Mode Under this mode once entering into the thermostatical state after reaching target Tube temperature and decreasing about 3 C reagents temperature can be close to target temperature very fast see Fig 2 13 Sample Volume Sample Volume ul 10 200 State of Hot Lid Off Off 14 Holid tat f Hotlid State o otli 30 110 Open 2 15 Key Sound SOUNA ER Yes No Humming 16 Run End Sound So Prd SONE Yes No Humming continuously or No 70 Appendix 2 BOECO TC PRO Thermal Cycler 0 a1njesaduaL Reag n Oo 9ine1aduial Fig 2 Fig 1 NOTE E BOECO Germany Boeckel Co GmbH Co KG P O Box 11 22 26 Roedingsmarkt 33 20459 Hamburg 20422 Hamburg Germany Tel 49 40 32 56 27 27 Fax 49 40 32 56 27 41 Website www boeco com
17. reason analysis and troubleshooting 1 Failure Analysis and Troubleshooting No Phenomenon Failure Analysis Troubleshooting Disconnected Check power supply for power supply correct connection Bad fuse Replace it 250V 8A 1 No display and the two beeps missed 5x20mm when remeron Switch failure Replace it Others After power on the instrument beeps Bad connection Power off pull out the twice and then the LCD display between the j i 2 block and then re insert Please insert Block with beep block and the the block and power on warning sound main body Error Message when running Please Power off pull out the check error with any information block and then re insert the below Bad sensor block and power on 3 Temperature sensor disconnect connection Or Temperature sensor1 disconnect sensor broken Contact distributor or Temperature sensor2 disconnect manufacturer Temperature sensor3 disconnect Power off pull out the Error Message when running Please Bad sensor block and then re insert the 4 check error with the information like connection or block and power on Heat sink sensor disconnect sensor broken Contact distributor or manufacturer Power off pull out the Error Message when running Please Bad sensor block and then re insert the 5 check error with the information like connection or block and power on Ho
18. remind Confirm stop running According to the reminding information to select STOP or START Press Pause and it will remind Now running According to the reminding information you can select START to continue If you don t make a decision within 100 seconds the system will pause continue running automatically Chapter Four Operation Guide File Running Interface BOECO Version 2007 10 A 2007 11 1 10 05 00 User BOECO Time m s Cyclel 00 00 01 01 00 Total Time 00h 00m Remain Time 00h 00m Hotlid 042C File Name PCR1 Seg 1 Temp C 32 6 SV 95 0 B Block X well Default User BOECO Default File PCR2 Control Mode Block View File Press View File to view edited PCR programs Press Skip to stop current temperature and run the next 3 2 How to set system parameters In the main interface press System to enter system parameter setting interface Press Enter to move the cursor to next item The option is followed by the symbol N Press Up Down to roll the option for selecting and other information can be entered information by pressing Digit amp Letter Press Right Left to move the cursor s position Press Back to go back to the main menu Press Save to save the parameters Special Reminding System Parameter Setting Interface BOECO Version 200
19. 000000000000000000 000000000000000000000000 000000000000000000000000 ee 90 e eo e e eo e e eo e e eo e e eo e e eo e e eo e e e e e eo 0 90 000000000000000000000000 000000000000000000000000 9000000000000000000000000 mn mg on H FER 30 5 35 0 44 2 52 8 31 1 36 3 53 4 31 4 38 0 47 3 53 7 31 7 39 7 49 9 54 0 BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Change the equipment IP and the name Choice the Server Setting option under tool menu pop up the setting dialog box Click the Add button to add a row Click the Delete button to delete the current row Put the ip address and alias click Save button to save the setup ee e a Clew 1 The setup will go into effect until the software restart 2 lp setup should be corresponding to the equipment Server Setting 192 168 0 222 192 168 0 243 Ek Delete 192 168 0 240 192 168 0 241 LJ Save Cf Close Chapter Six Firmware Upgrade 1 Configure your equipment Connect the equipment and computer lines through the network cross then Class B RJ45 connection Computer Instrument 2 How to Upgrade the Software 1 Open apparatus power to start instrument hold down the F1 key at the same time then equipment enter the software upgrade readiness estate the apparatus display Prepare to upgrade e 2 Operate the procedure Network Enable Adminastrator Equipment will use vir
20. 007 11 1 this time the File Name Column will display the relative files of the selected user dynamically Shift to the File Name Column where the flashing bar will flash then select relative user s file through pop up key Press Edit to edit the file indicated by the cursor New File Delete Press New File to edit a new file Press Delete and the system will alarm Confirm delete file then according to alert to select Delete or BACK Press Back to go back to the main interface If the current Control Mode is Block mode press Run to enter into the File Running Interface While if the current Control Mode is Tube mode press Run thus the following dialog box will be displayed Select Sample Volume 0501 1 The user can input Sample Volume Unit Micro liter by the same way as that of the main interface Press Back to go back to File List Interface Press Run to enter into the file running interface Note The sign at the top of User and File indicates the current running file is default one which can be modified in the parameter setting interface BOECO TC PRO Thermal Cycler In this interface move cursor by pressing Up Down Right Left and the position that the cursor reaches will be displayed in white on a black background Press Digit amp Letter to modify parameter settings Press Functi
21. 1 Status Data Mode View Settings Settings Click the Modify check box to modify configuration Y DK x Cancel Open Operrating Modemenu click settings open the property page to choice operrating mode and click setting button Configuration E Information Auto Waming IP Address Report Password DigitallO Serial CMD MESA Basic Network Serial Operating Mode Accessible IPs NE 41105 MAC Address Iv Modify 00 30 E8 10 E2 B7 EIER OP Mode rr IE Real COM Mode Serial Number 3898 Firmware Wer Ver 4 0 BIOS Ver Ver 2 1 Status Data Made Click the Modify check box to modify configuration Y OK x Cancel Choice Real Com Mode in operation Mode Operating Node 1 Part s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode TCP Server Mode hoice Real m TCP Server Choice Real Co UDP Mode Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 14001 TCP Alive Check Timeout f 0 99 min Inactivity Timeout Max Connection iD 0 565535 ms Data Packing Optional Delimiter 1 00 D4f Hex Delimiter 2 00 Of Hex Force Tx Timeout O 065535ms Y OK X Cancel BOECO TC PRO Thermal Cycler Click the function list COM MAPPING option N Network Enabler Administrator CON Mapping File Function COM Mapping View Help Chapter Six Firmware Upgrade EEK
22. 2 Keyboard DIagratm iu od Ao oleo AL 9 3 Keyboard Instructions iaaaaaaasiiaaaaaannssannnnannnnnnannnnnnnnnnnnnnnannnnnnnsnanannnnnnnsnanaannnnnnnananaaaaa 9 4 Installation of Block Un 10 E ME UE ele ET 10 4 2 Dismantlement Steps AE 11 5 Inspections before Power on aaaaaiaaaaaaaaaaasaaaaaaannsannnanaannnsnnnnnnnnnnnnnnaanannnnnnnannnannnnnnsanaaanaa 12 6 EE 12 Chapter Four Operation Guide HH HH HH TS kh 13 VBK 13 2 How to run a single block PCR program lt lt 11111111 ng kh 14 2 1 How to edit PCR programs aust ateist 16 2 2 How to set system paramef rS HH HH HT TH kh 20 3 How to run a double block PCR program 22 3 1 How to edit a PCR program 1111 ng 23 3 2 How to set system parameters aaaaaaaaaiaasaaannannnsannnnnnnnnnsnnnnnnnnnnnssnnnnnnnnnnnsnnna 26 4 How to run a gradient block PCR program 27 BOECO TC PRO Thermal Cycler Contents 4 1 How to edit a PCR program 28 4 2 How to set system parameters aaaaaasaiiaaaaaaasssnnanannnnnsannanannnnnsannnannnnnnaaanaaaaaa 31 4 3 Characteristics of Temperature Distributing of Gradient Block 31 5 How to Input and Output the PCR Program aiaaaaaaaasss saaaaass asaaaaaaanaaanaaaaaaanaaanaaaaaa 32 5 1 Connect Usb Flash Memory aaaaaaaiaaaaaaaaas s
23. 2 3 Control Mode states the default control mode including two optional modes as Block and Tube Block mode subjects to simple temperature control during the course of cooling amp heating only Compared to the block cooling amp heating rate of reagent is a little slower therefore the actual change of reagent s temperature is different from the setting procedure The actual time for reagent to reach 8 keep the setting temperature is far shorter than the setting time Please refer to the following figure relation between block temperature and reagent temperature 100 e JJ HIT TT Bleck 94 E a Reagent Temperature C Time s Tube mode has considered the phenomenon that the cooling amp heating rate is a little slower than that of block There is an overshoot after reaching the setting temperature which can allow reagent to reach the setting temperature faster During the same setting time as Block the actual time for reagent to keep the setting temperature is prolonged obviously Please refer to the following figure relation between block temperature and reagent temperature 100 97 94 91 88 85 82 79 76 73 Block Reagent Temperature C 2 2 4 Hotlid stand for the state of hotlid Open or Close 2 2 5 Key Sound Run
24. 30 E8 10 E2 B7 Serial Number 3898 Firmware Wer Ver4 0 BIOS Ver Ver 2 1 Status Data Mode Auto Warning IP Address Report Pot Alias OP Mode Vs F a COM Mode Ey Password DigitallO SerialCMD Operating Mode Accessible IPs Settings Click the Modify check box to modify configuration Operating mode choice TCP Server Mode Operating Mode 1 Port s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode j TEP Server Mode TCP Client Mode UDP Mode TCP Server Mode Settings Local TCP Port TCP Server 4001 Max Connection vw OK X Cancel Choice TCP Server Mode Miscellaneous Optional TCP Alive Check Timeout 7 0 99 min Inactivity Timeout io 065535 ms Data Packing Optional Dt Hex 0 ff Hex Delimiter 1 Delimiter 2 Force Tx Timeout 0 65535 ms Click Ok button close Network Enable Adminastrator Ao X Cancel 12 Restart the apparatus after closing enter the system parameter configuration interface to reinstall system parameter Note Upgrade procedures will not be noticed in additional users can land website www BOECO com and download the latest version for the procedure BOECO TC PRO Thermal Cycler Chapter Seven Failure Analysis and Troubleshooting Chapter Seven Failure Analysis and Troubleshooting In this chapter we briefly represent possible failures
25. 7 1 0 A 2007 11 1 10 05 00 Default User Dlsigdele N Default File lei Control Mode N Date RENTE Sample Volume Time T N Key Sound N Hotlid DES Run End Sound Yes fi B Block X well Default User BOECO Default File PCR2 Control Mode Block When set the parameter please press the Save otherwise the parameter remains the original one BOECO TC PRO Thermal Cycler Chapter Four Operation Guide NOTE After set the parameters please press Save to confirm setting otherwise the parameter remains the original one Before the instrument leaves the factory its inside clock has been adjusted in accordance with Beijing Time For the first use of the instrument you are advised to confirm whether the date and clock time are set right as local time 4 How to run a gradient block PCR program Switch the power on BOECO TC PRO Thermal Cycler will beep twice and it shows the power supply is connected well The screen will display Self testing and the instrument will carry on self testing The self testing needs about 1 2 minutes and please wait patiently If the self testing is successful the screen will display the main interface Main Interface On the left top of the main interface the state of Hot lid is displayed On the central area basic information such as Boeckel Co GmbH Co KG information software version number block type default file BO
26. BI FA BUS Sun KEN Bl MKV FT usa wmm SI ru MD FT hss sak Han E Ei BSU y 100 r W I8 Wei Dei Ki t Wl soup EBP En _NW E mm KI o WT RE Ri h i d yen EL OMIT ko y i 10024 Eo am RE REC L ui l W th E RE N I kj E S BOECO TC PRO Thermal Cycler Appendix 2 Appendix2 Parameter Value amp Meaning Ite m Parameter Meaning Range Note 1 Temp C Temp Control point 4 0 99 0 gt The time of Temp control is 2 Ti Time of Temp set the value before long limitlessly meanwhile the ime Control mm ss through Function key hotlid closed 00 00 59 59 Ramping Rate 0 1 2 0 2 Same CC Second HH Maximum of Ramping Rate Temp Gain of Every 8 4 Temp Cycler 0 0 9 9 4 Time Gain of Every 5 Time Cycler mm ss 0 00 9 59 Cycle1 Section Numbers 1 5 Cycler Numbers from _ x00 beginning to the end 1 99 6 The start segment XCand existed segment Cycle1 x00 From 00 to 00 From 00 of the section no to 00 The end segment lt X 15 of the section Gradient Temp 7 Grad Difference CC 0 30 8 Date Date YY MM DD 00 99 01 12 01 31 year month date 9 Time Time HH MM SS 00 00 00 23 59 59 hour minute second 10 Default File The default file Max length is 11 name bytes 11 Default User The default user Max length is 11 name characters Under this mode once
27. H Co KG Version 2007 1 0 Block kis ke Default User BOECO Default File PCR1 Control Mode Block screen shows the following interface BOECO TC PRO Thermal Cycler In this interface press Number key to input code lt gt lt gt Press Back to go back to the main interface Press Accept key to confirm the code If the module is mixed block press Accept to determine the test tube type pop up tube type selection dialog If the module is not mixed block the current Control Mode is Block mode and press Accept running into the file operation interface If the current control mode is Tube mode press Hot lid off Chapter Four Operation Guide Main Interface 2007 11 1 10 05 00 BOECO Boeckel Co GmbH Co KG Version 2007 1 0 Block TUA Default User BOECO Default File PCR1 Control Mada ha Input password Accept key then pop up tube vol Settings dialog box In this interface press can choice the actual tube type gt Press Back key to go back to the main interface Press next If the current control mode is Block Mode click next to enter document operation interface if the current mode is Tube Mode press next key pops up the following interface Hotlid off Tube style selection 2007 11 1 10 05 00 BOECO Boeckel Co GmbH Co KG Version 2007 1 0 Block m Def
28. HTLV HIV HBV HCV HPVS EV CMV EBV HSV measles virus rotiform virus B19 virus and Lhasa virus Parasite malaria Human hereditary diseases Lesh Nyhan syndrome hemophilia BMD and DMD HLA locus typing qualitative analysis of T cell receptor or antibody diversification immune body gene mapping quantitative analysis of lymph genes Identification of DNA markers using discrete repetitive Sequence construction of a genetic linkage map detection of DNA polymorphism or semen mapping Construction of a physical map sequencing and map expression BOECO TC PRO Thermal Cycler Chapter One Introduction Forensics Specimen analysis in the venue and HLA DQg locus typing Oncology Pancreas cancer rectum cancer lung cancer thyroid gland cancer melanin cancer and leukemia Social and Genetic species research evolution research animal protection Colony Biology research ecology environmental sciences and experimental genetics Paleontology Specimen analysis in archeology and museum Biology Diagnosis of animal hereditary diseases and detection of plant pathogeny 3 Features of BOECO TC PRO BOECO TC PRO Thermal Cycler is a PCR detection system with multiple functions It has features and characteristics as followings Provide five kinds of interchangeable block units to meet various PCR experiments requirements Adopting American ITI advanced thermoelectric cooling technology and thermoelectric cooling comp
29. Key beep Temperature control mode Default user Default file Module Default hotlid temperature Beep alarm when run finish Key beep Temperature control mode Default user Default file Module B Default hotlid temperature Beep alarm when run finish Key beep Temperature control mode Default user Default file E riter fan gt eql06 s x eq104 96G s gt BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 8 User manual How 2 Create new users 2 Change the user name 2 Copy user 2 Paste user 2 Delete user 2 Imput user 2 Export user Create a new user 1 Choose the name of the equipment list or any user name 2 Choice the create user menu in User menu pop up user dialog box 3 Enter your user name click ok button Create User Do you want to create new user in Computer Enter new user name The user name can contain only letters and numbers L OK x Cancel BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Change the user name 1 Choice change the user name option 2 Choice the Change User Name menu under the User menu pop up the Change User Name dialog box 3 Import user name click the ok button Clew User can t change the user name when the file of this user is opening Change User Name Do you want to change user name of Computer s 123 Enter new user name
30. Next Page Copy BOECO TC PRO Thermal Cycler 5 3 Export file to usb flash memory Press Back key to return back the usb link flash memory interface lt Press cursor up down moving key to choice the copay file user If the user number bigger than 30 press cursor left right key to previous next page Press view key to display usb file list Press Back key can return back to equipment user list interface lt Press cursor up down moving key to choice the copay file lt If the file number bigger than 30 press cursor left right key to previous next page If copy correct display the following dialog box Press view key to display usb file list File Saved Otherwise display File output error Chapter Four Operation Guide Equipment User List Hotlid off 2007 11 1 10 05 00 Userl User2 Press Left Right Key to Previous Next Page View Equipment File List Hotlid off 2007 11 1 10 05 00 Local Filel File2 Press Left Right Key to Previous Next Page Copy BOECO TC PRO Thermal Cycler Chapter Four Operation Guide Note Usb read write user number is 500 every user s file number is 2000 Usb read write fixup list usb root list descended BOECO folder Every user is one son folder of BOECO folder Forbi
31. Right Left moving the cursor one position to the left or right when pressing during file parameter setting Up Down moving the cursor one position to the up or down when pressing during file parameter setting Enter press it to accept the present parameters setting displayed on the screen when setting documents eq eo ea ea 55 Menu press any button to accept the current items displayed EY E2 E3 4 FS above the button 4 Installation of Block Unit The main unit must link with the block unit so as to work normally Please install or dismantle the block unit according to the following steps 4 1 Installation steps a Putthe block onto the slot of main body uplift the locking handle and push the block slowly until the locking handle is in the right place b Press the locking handle downwards thus the block will move forwards automatically The sound kaka means that the block is installed in the right place c Power on and the instrument can run normally BOECO TC PRO Thermal Cycler Chapter Three Preparations Main Body Main Unit Connecting Plug Locating set Block Unlocking Device Rotator Locking Handle Note 1 Make sure to switch off the power supply before installing block 2 When install block please pay close attention to push the block slowly Otherwise the main unit connecting plug may be damaged 3 When uninstall block please draw the block
32. aaaanaanssanaaannanannaannanannnnnsnanaaanannnnaania 32 5 2 Input file from usb flash memory aiaaaaaaaaasasaaaaaannssanannnnannnsaanannnnnnnsannnannnnnnaaania 33 5 3 Export file to usb flash memory m s r nnne 34 Chapter Five Connection of Computer 36 T1 o9itware nstallatiora sanatione o 36 2 Online Setup a et 36 3 Software installations rtt re t rt tele iU id ft n i i ie 36 4 Contigure ee e eu 5 Wu 38 4 1 Network ConnecfiOn 000001100300 2111111101100 1 11g ng cu 38 4 2 Network Enable Adminaestraior S221 H HH Tnhh 39 ACOu e e baa 40 b Main interfacec ooo Ne 44 o Te eT 45 7 Basic manipulation E 45 SE EE Ee D satte 48 9 File menu ENEE 50 10 TOOL MENU sanere ten u tu de eite tee entente tel de eate er Eds 55 Chapter Six Firmware Upgrade iiaaaaaaaaaiaaaaaaaass nnaanaannnsaaaaaanannnsnanannnnnnnnnananannnnnaaanaaaaaa 58 1 Configure your eguipment nan nemen nnn 0xx nenen 58 2 How to Upgrade the Gottware nro H snnt 58 Chapter Seven Failure Analysis and Troublesbootng conce 66 1 Failure Analysis and Troublesbhootmg nn nh 66 2 Spare Parts list o EE 68 Appendix 1 Wiring DIgEam iiretete tihi ska e iter ene Ee p ee Baka kana id 69 Appendix 2 Parameter Value amp Meaning nnn 70 BOECO TC PRO Thermal Cycler Chapter One Introduction Chapter One Introduction This chapter briefly introduces PCR technique and its applications PCR thermal cycle
33. ault User BOECO Default File PCR1 Control Mode Tube Select Tube Type 02ml next BOECO TC PRO Thermal Cycler Chapter Four Operation Guide In this interface press Character key to input id 11 1 10 05 number of sample volume Unit Micro liter Hotid off 007 11 1 10 05 00 according to the practicality Under Tube Mode B O E CO samples with different concentrations have different overshooting temperature and duration Boeckel Co GmbH Co KG time Version 2007 1 0 Block de Press Back to go back to the main Default User BOECO interface Default File PCR1 Control Mode Tube lt gt Press Run to enter into file running interface Select Sample Volume 0501 1 next 2 1 How to edit PCR programs File List Interface Press File on the main interface to enter file list interface There are 3 files listed in the form Hotlid off 2007 11 1 10 05 00 as showed in the picture When the flashing bar No User File Name Save Time i ina i 1 PCR1 2007 11 1 flash th lect is flashing in the User Column select users 2 BOECO2 2007 11 1 through pop up key at this time the File Name 3 BOECO3 PCR3 2007 11 1 Column will display the relative files of the selected user dynamically Shift to the File Name Column where the flashing bar will flash then select relative user s file through pop up key Press
34. ault User BOECO change control symbol In the central Default File PCR1 area of the main interface it displays PENNEN the types of A amp B blocks default file E gt default user control mode and sample velan Block X well If the current Control Mode is Block Default Use BOECO mode press Run to enter into the Default File PCR2 File Running Interface While if the Control Mode Block current Control Mode is Tube mode self testingeeeees press Run and thus the following E _ dialog box will appear Press File to enter into the File List Interface Press System to enter into System Parameter Setting Interface Ifthe current file doesn t have code press Run to enter into File Running Interface and tube type confirmation If the current file has code press Run thus there appears a dialog box Select Sample Volume 0501 I BOECO TC PRO Thermal Cycler Chapter Four Operation Guide The same as single module Press Back to go back to the main interface Press Accept key to confirm the code If the current Control Mode is block mode press Run running into the file operation interface If the current control mode is Tube mode press Run key then pop up the following dialog box Select Sample Volume 0501 I The same as single module user could input the sample volume Unit Micro liter according to the practicality Press Back to go back to
35. cation 1 Choice the local computer user option 2 Choice Export File menu under the File menu 3 Choice the file click open button Clew Can t imput the opened file Export File 1 Choice the file need to export 2 Choice the Export File men under File menu 3 Choice the export outlet click OK button BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Clew Can t export the opened file 10 Tool menu How 2 Connect equipment 2 Disconnection 2 Grads calculate 2 Change equipment ip and name Connect equipment Choice the equipment click Connect menu Clew One equipment can only connect one computer at the same time Disconnection Choice the equipment click the Disconnect menu Choice the grads menu Import the grads origination point and the grads value Choice the computer module type 8x12 96well 16x24 384well Click Calc Button pede gu BOECO TC PRO Thermal Cycler Chapter Five Connection Computer HEG User File Tools Help 2289282 0000082004537 B Hu Tara Temp a Fiterlan y B 34 Temp 30 Gredientpa 8x12 c16x24 tale Colder Hotter a TC PRO User File Tools Help 82 828 3202008aR0R0 TT B ale og rA Grad Temp g Filter fan gt 5 8 12 a 34 Temp 30 Gradient 24 cex12 16X24 Calc Colder Hotter 000000
36. d to insert and pull out usb flash memory very fast To protect apparatus and usb flash memory if insert and pull out usb flash memory the apparatus will close usb function until the next boot strap The operation method for double module is the same the link of usb flash memory is only for two module under the original interface BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Chapter Five Connection of Computer 1 Software installation Click Setup exe to Installation TC PRO Program 2 Online setup TC PRO has configurable embedded internal networking modules provide the corresponding tools set 3 Software installation The first step click to run need setup exe T Setup Network Enabler Administrator EJ x Welcome to the Network Enabler Administrator Setup Wizard This will install Network Enabler Administrator 2 11 on your computer It is recommended that you close all other applications before continuing Click Nest to continue or Cancel to exit Setup Next gt Cancel The second step click next button al Setup Network Enabler Administrator Select Destination Location Where should Network Enabler Administrator be installed O Setup will install Network Enabler Administrator into the following folder To continue click Next If you would like to select a different folder click Browse Browse C Program Files Network Enabler Administrator At
37. ex Delimiter 2 00 0 ff Hex Force T Timeout ER 0 65535 ms wv OK X Cancel Single click ok button finish IP address setting BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Single click function list COM MAPPING Ny Network Enabler Administrator COM Mapping File Function COM Mapping View Help Add object equipment Configuration d Monitor Port Monitor P Address Report Delete object equipment 2008 1 3 14 26 33 2008 1 3 14 Configuration Changed 2008 1 3 14 Configuration Chang 2008 1 314 Configuration Changed NE 41105 01 2008 1 3 14 Configuration Changed NE 41105 00 90 E8 1027 Network Enabler Administrator CON Mapping File Function COM Mapping View Help DOAS i COM Mapping 1 COM Network Enabler Admir Configuration None 8 jf COM Mapping Monitor Port Monitor IP Address Report Found Network Enabler Module s 1 Configuration Changed NE 41105 00 2008 1 3 14 35 39 Configuration Changed NE 41105 00 2008 1 3 14 35 49 Configuration Changed NE 41105 00 1 2008 1 3 14 37 22 Configuration Changed NE 41105 00 3DEB10E287 BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Double click on the column searched IP setting the mapping attributes page select Advanced Settings setup as the following fig COM Port Settings Port Number 1 Port s Selected 1st port
38. f chip with its socket Chip malfunctioning Contact distributor Or manufacturer Inactive keys Film panel destroyed Contact distributor Or manufacturer The setup of parameters is invalid Failure to press Save button after the setup of parameters Please refer to Section 1 2 2 2 and 3 2 of Chapter 4 BOECO TC PRO Thermal Cycler Chapter Seven Failure Analysis and Troubleshooting Failure to set Please refer to How to set hot lid the parameters in the temperature but Section 1 2 2 2 3 2 of set the hot lid Chapter 4 to set the as OFF temperature of hot lid 43 Reagent in the reaction tubes The reaction Adjust the hole location of evaporated tubes are placed reaction tubes and place unevenly the tubes symmetrically The lid of Please close the lid of reaction tubes tubes tightly before put are closed them into the instrument untightly Note During warranty terms please contact distributor or manufacturer if there is a need to open the device to check a failure listed in the above table Each instrument with the same or different brand has their own control temperature features including cooling amp heating rate stability and fluctuation meanwhile the biological experiment has its own features such as indeterminacy and easy to be affected by the surroundings therefore any PCR program that is achieved on one instrument success
39. from the slot gently It s forbidden to lean the block for fear of snap of connection wire 4 After installation the block unit will be recognized by the main body automatically and will be ready for work without any other operation 4 2 Dismantlement Steps a Power off firstly make sure to dismantle or replace the block b Uplift the locking handle it will sound kaka and the block will move backwards simultaneously c Take out the block through the locking handle The dismantlement is finished BOECO TC PRO Thermal Cycler Chapter Three Preparations A d F II e 5 Inspections before Power on Before switch the power on please ensure that 1 Supply voltage falls within the specified limits refer to Chapter Two 2 The plug has been inserted into the power socket reliably 3 The grounding of the power line is reliable 6 Power on Turn on the power i e turn the power switch to position the BOECO TC PRO Thermal Cycler will beep twice and the fan start to running which means the instrument is power on At this time he LCD will display Self testing which means the instrument is under self testing It will last for about 1 2 minutes Please wait patiently After the self testing the main menu will be displayed and the instrument is ready for operations such as editing accessing modifying or deleting a PCR amplification file BOECO TC PRO Thermal Cycler Chapter
40. fully maybe cannot be achieved the same successful result Once you change instruments to do the same experiment you d better adjust the PCR running program so as to get an ideal state 2 Spare Parts list NO Symbol Name 1 BYQ505801100000 Driving board 2 BYQ505801200000 CPU board 3 BYQ505801410000 Transform parts 4 BYQ605802000000 Block parts 5 BYQ154314200010 Fuse 6 BYQ165200000020 Resistance 7 BYQ902102100070 Peltier 8 BYQ152303000250 Power Plug 9 BYQ155420000070 LED display 10 BYQ905801300010 Touch panel 11 BYQ158206000040 Power supply 12 BYQ905801300020 Touch panel shell BOECO TC PRO Thermal Cycler Appendix 1 Wiring Diagram Appendix 1 This diagram is regarded only as a reference and it is a subject to change without prior notice We apologize for any inconvenience caused ORR SIS Driver Board RE Conect Board AND RB OU Bead i DE HH h pamm Ln TTTlmtt HE FT 1 DIN I3 HE ae KE FT UE NE H bag 0 A 61 H t m li Koap H 108 Ham d Et HCH On DER Wi bam DE m gs ORA jar K t ML cn IODE ppm Y UN
41. he Thermal Cycler complying with the National Standard GB4793 1 is a general instrument of class II the protection degree is IP20 It is intended for indoor use a Grounding Considerations A C power s grounding should be grounded reliably for fear of an electric shock The 3 pin plug with the Thermal Cycler s power line is a safety device that should be matched with a grounded socket Never let the third ground pin floating If the 3 pin plug cannot be inserted it is recommended to ask an electrician to install an appropriate power socket b Keep Away from Electric Circuits The operator is not allowed to open the Thermal Cycler To changing components or adjust certain parameters inside the device must only be accomplished by the certificated professional maintenance personnel Do not change elements while the power is still on c A C Power Considerations Before turn on the power always check if the mains voltage is within the required A C 10 difference is allowed and whether the current rating of the power socket meet the required specification d A C Power Line Considerations As an accessory of the Thermal Cycler the A C power line used should be a default one If BOECO TC PRO Thermal Cycler it is damaged the A C power line may not be repaired but could be replaced by a new one with same model and specification The power line should be free of heavy objects during the Thermal Cycler s operation Keep the power li
42. ing into the file operation interface If the current control mode is Tube mode press Accept key then pop up tube vol Settings dialog box BOECO3 PCR3 2003 11 1 Block x well Default User BOECO Default File PCR2 Control Mode Block New File Delete BOECO TC PRO Thermal Cycler Chapter Four Operation Guide If the current Control Mode is Block mode press Run to enter into the File Running Interface while if the current Control Mode is Tube mode press Run and thus the following dialog box will appear Select Sample Volume 0501 I The user can input Sample Volume Unit Micro liter by the same way as that of the main interface Press Back to go back to File List Interface Press Run to enter into file running interface NOTE The sign at the top of User and File indicates the current running file is default one which can be modified in the parameter setting interface File Edit Interface In this interface move the cursor by pressing Up Down and Right Left and BOECO Version 2007 10 A 2007 11 1 10 05 00 the position that the cursor reaches will be skeleton 1c 01 00 HH displayed in white on a black background 22 550 02 00 HH Press Digit amp Letter to modify parameter 3c 72 0 01 45 settings Press Function to change 4 040 00 00 Press Seg to enter into the Segment Edit Interface In every Cycle1
43. inue to save Save If the module is mixed block determine the test tube type first pop up the following dialog Select Tube Type 0 2ml lt Password setting press number memory lt gt box lt gt If the current Control Mode is block mode press Run running into the file operation interface If the current control mode is Tube mode press Run key then pop up the following dialog box Select Sample Volume 0501 1 The same as main interface user could input the actual password sample type and number of sample volume Unit Micro liter according to the practicality Note 1 No difference between big and small letter in setting file name and user name 2 Please remenber the setting password 3 Setting the password as 0 9 number character BOECO TC PRO Thermal Cycler During normal running of a file it will be flashing at the position of Now Running When the file is running over the system will alarm File run over Press Stop and it will remind Confirm stop running Please select Stop or Start according to the reminding information Press Pause and it will remind Now pause running According to the reminding information the user can select Start to continue If the user doesn t make a decision within 100 Chapter Four Operation Guide File Running Interface Hotlid off 2007 11 1 10 05 00 le 95 0 1 00 File Name PCRI User BOECO Ic Temp
44. is Port 1 Basic Settings Advanced Settings Serial Parameters Tx Mode Classical FIFO Enable Fast Flush Only Flush Local Buffer Apply All Selected Ports OK X Cancel Choice Serial Parameters setup as the following fig CON Port Settings Port Number 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters bauinac ESE Parity Data Bits Stop Bits Flow Control X Cancel BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Click ok button and click OK button of the application settings and click the Remove equipment completed mapping settings Note 1 2 3 4 5 The IP address is not unanimous when multiple devices interconnection In the first use re equipped the equipment Need to set up the same subnet when multiple equipment networking Device does not support automatic ip address functions Multiple equipment networking can use switches such as the use of cross HUB requires the use of network connectivity 5 Main interface a TC PRO eq106 User 54 System th History GP Setting Filter all v eq10B 5 v eq104 96G S Equipment list 44 BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 6 Toolbar Amend user name Input file Copy file Change file code Grads calculate Output user Paste user New file Delet file Link equipment y
45. ital 10 Serial CMD PM um ES Network Serial OperatingMode AccessiblelPs v Modify MAC Address I 1 D 30810 2 7 IP Address Tb Input IP address Serial Number Netmask gt Input netmash Gateway Firmware Ver ee Ver 4 0 IP Configuration Static DNS Server 1 BIOS Ver Ver 2 1 DNS Server 2 Status Modify Data Mode Community Name Click the Modify check box to modify configuration Y OK x Cancel BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Open the attribute page and choice operrating mode option then click setting button x Configuration Information Auto Waring IP Address Report Password Digital ID Serial CMD NE Basic Network Serial Operating Mode Accessible IPs lv Modify MAC Address 00 30 E8 10 E2 B7 Pot Alias OP Mode max COM Mode Serial Number 3898 Firmware Wer Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Click the Modify check box to modify configuration Y OK x Cancel The operating mode choice TCP server mode Operating Node 1 Part s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode A Choose TCP TCP Server Mode sa 2 Miscellaneous Optional Server Mode Local TCP Port 400 TCP Alive Check Timeout 0 99 min Inactivity Timeout Max Connection 0 0 65535 ms Data Packing Optional Delimiter 1 00 Of H
46. k Press F4 to Input File Press F5 to Output File Back Input If no inserted usb flash memory display as the left fig Press Back key can return back to the main interface 5 2 Input file from usb flash memory lt gt Press Back key can return back to the usb link flash memory interface Press cursor up down moving key to choice the copay file user If the user number bigger than 30 press cursor left right key to previous next page Press view key to display usb file list Press Back key can return back to usb flash memory user list interface lt gt lt gt Press cursor up down moving key to choice the copay file If the file number bigger than 30 press cursor left right key to previous next page Press copy key to input file If copy correct display the following dialog box File Saved otherwise display File input error BOECO TC PRO Thermal Cycler Chapter Four Operation Guide Usb Link Error Interface Hotlid off 2007 11 1 10 05 00 No disk connection Press F1 to Back Back Usb User List Hotlid off 2007 11 1 10 05 00 Userl User2 Press Left Right Key to Previous Next Page Back View Usb File Fist Hotlid off 2007 11 1 10 05 00 Filel File2 Press Left Right Key to Previous
47. least 2 5 MB of free disk space is required BOECO TC PRO Thermal Cycler Chapter Five Connection Computer The third step choice installtion list click next button a Setup Network Enabler Administrator Select Additional Tasks Which additional tasks should be performed Select the additional tasks you would like Setup to perform while installing Network Enabler Administrator then click Next Additional icons The fourth step click next button al Setup Network Enabler Administrator Ready to Install Setup is now ready to begin installing Network Enabler amp dministrator on your computer Click Install to continue with the installation or click Back if you want to review or change any settings Destination location C Program Files Network Enabler Administrator Additional tasks Additional icons Create a desktop icon Install Cancel BOECO TC PRO Thermal Cycler Chapter Five Connection Computer The fifths click install button a Setup Network Enabler Administrator Installing Please wait while Setup installs Network Enabler Administrator on your computer Extracting files C Program Files Network Enabler Administrator NEAD MIN exe The sixth step installation finished clich finish button al Setup Network Enabler Administrator Completing the Network Enabler Administrator Setup Wizard Setup has finished installing Network Enabler Administrat
48. m whether the date and clock time are set right as local time 4 3 Characteristics of Temperature Distributing of Gradient Block The gradient block adopts 3 group temperature control units so that the gradient temperature appears curve distribution as showed in the following figure Setting temp is 40 C and gradient setting temp is 20 The gradient distribution among every block s hole temperature is not even the temperature difference in mid of block is larger than that of its outer Therefore please refer to the temperature displayed on the machine for every hole s temperature when operating gradient block BOECO TC PRO Thermal Cycler Chapter Four Operation Guide Temperature C 4 0 T T T T Gradient graphs Colum n left to right T T T T T T 7 8 9 10 11 12 5 How to Input and Output the PCR Program 5 1 Connect Usb Flash Memory Press usblink key in the main interface to enter the usb usblink interface If insert usb flash memory display as the left fig lt Press Back to go back to the main interface Press Input key to choice input file from usb flash memory the equipment display usb user list lt Press output key to choice export file to usb flash memory the equipment display the local file user list Usb Link Interface Hotlid off 2007 11 1 10 05 00 The Insturment has Connected UsbDisk Please Choose Input or Output File Press F1 to Bac
49. ne away from the place where people gather regularly e Connect the A C Power Line While connect or disconnect the power line you should hold the 3 pin plug with your hand Insert the plug thoroughly to ensure good contact between the plug and socket Pull the plug but not the line when you need to disconnect to the mains f Requested Environments The Thermal Cycler should be placed in a low humidity dust free and good ventilation room without caustic gas or powerful magnetic interference In addition water sources such as pools and water pipes should be kept a distance from the Thermal Cycler Never cover or obstruct the openings of the Thermal Cycler which are designed for ventilation and prevent the device s interior from being too hot When a single device is running the shortest distance between its openings and the nearest object is 50cm when two or more devices are running at the same time the shortest distance is 100 cm Do not place the device on a soft surface for that will result in adverse ventilation near the device s bottom openings Too high temperature will lead to degraded performance or failure of the Thermal Cycler Therefore the device should be protected against any kind of heat sources like sunlight ovens or central heating equipment If the Thermal Cycler is set aside for a long time it is recommended to disconnect the power line to mains and cover the device with a piece of soft cloth or plastic to preve
50. nt dust from entering Note Once one of the following events occurs you are suggested to disconnect the power line with mains and contact the distributor or ask a certificated maintenance personnel for help Liquid into the device The device sprinkled or drenched The device malfunctioning giving off abnormal sound or odor The device falling onto the floor or its shell damaged Significant changes in the device s performance BOECO TC PRO Thermal Cycler 3 LABEL a Tablet BOECO C BOECO Name Thermal Cycler Name BLOCK 96G Model TC PRO Power 100 240V 50 60Hz 600W Fuse 250V 8A 5X20 Sort class II IP20 SN kkkkkkk kkk Model B 96G SN KKKKKKK KKK Date KA Date kkkk RK Boeckel Co GmbH Co KG Hamburg Germany b Warning Sign AN HOT SURFACE Warning There are two warning signs read HOT SURFACE During the program execution and in the short period after the program execution the metal part near those signs on the block and hot lid is not allowed to be touched by any part of the body for fear of the body burn 4 MAINTENANCE The conical holes over the block should be cleaned regularly with soaked cotton swab in order to ensure sufficient contact and thus good heat conduction between each conical hole and the tube inside it BOECO TC PRO Thermal Cycler Warning When cleaning the instrument should be off Corrosive scour is not allowed to clean the surface of
51. nter clockwise for two turns thus to loose the unlocking device to revolve the knob 1 Block BOECO TC PRO has five models with different purpose And each model has its own running program wes B96G B3048 B384G Gradient block program Mixed block program Gradient block program BOECO TC PRO Thermal Cycler B48D Dual block program Chapter Four Operation Guide O B4I Single block program 2 How to run a single block PCR program Switch the power on BOECO TC PRO Thermal Cycler will beep twice and it means the power supply is connected well The screen will display Self testing and the instrument will carry on self testing The self testing needs about 1 2 minutes and please wait patiently If the self testing is successful the screen will display the main interface On the left top of the main interface it displays the state of Hot lid On the central area it displays Boeckel Co GmbH Co KG information software version number block model default file default user control mode and sample volume Press File to enter into file list interface Press System to enter into system parameter setting interface lf the current file doesn t have code press Run to enter into running interface and tube mode confirmation lf the current Control Mode has code press Run thus the Main Interface Hotlid 0ff 2007 11 1 10 05 00 BOECO Boeckel Co Gmb
52. nue to save Save If the current Control Mode is Block mode press Run to enter into the File Running Interface While if the current Control Mode is Tube mode press Run thus the following dialog box will be displayed Select Sample Volume 0501 I The user can input Sample Volume Unit Micro liter by the same way as that of the main interface Press Back to go back to File List Interface Press Run to enter into the file running interface Note Setting the password as 0 9 number character File Running Interface When the file is normally running Tess at Hotlid 0ff 2007 11 1 10 05 00 Now Running will continuously flash When running is over the system will remind File run over Press Stop and it will remind DI d H 2 Confirm stop running According to EM the reminding information to select STOP or START If the user make File Name PCRI User BOECO Now Running no choice the system will continue to le Time 00 00 Cyclel 01 of 10 run within 100 seconds automatically 1 2 3 4 5 6 7 8 9 10 11 12 Press Pause and it will remind Now 95 0 95 1 p5 2 ps 4 5 7 p5 9 pe 1 p6 4 b6 6 P6 8 06 9 97 0 pause running According to the Total Time 00h 56m Remaining Time 00h 56m Pause Start View File Stop Skip BOECO TC PRO Thermal Cycler Chapter Four Operation Guide reminding information you
53. on to change lt Press Seg to enter segment edit interface In every segment you can set setting temperature Temp duration Time Ramping rate Ramp temperature increment of every cycler Temp and time increment of every cycler Time Press Delete to delete the present segment Press Cycle to enter section edit interface where you can set cycle numbers and initial cycle segment The figure shows a cycle from the first segment to the third one and there are totally 10 times cycles Press Cycle repeatedly to add sections max 5 sections Press Delete delete the segment Move cursor by pressing Up Down the former segment or the latter and to present to enter picture lt gt Special Warning Press Back to enter file run interface Hotlid 0ff Chapter Four Operation Guide File Edit Interface 2007 11 1 10 05 00 I i 2c 55 0 3c 72 0 4 04 0 02 00 01 45 00 00 Grad 02 02 02 00 Time 01 00 02 00 01 45 00 00 Ramp C s Temp Time 0 0 0 00 0 0 0 00 0 0 0 00 0 0 0 00 HH Cyclel X10 From01to 03 Press Enter To View Delails Delete Back Save 2007 11 1 10 05 00 Temp Time lc 1 1c 95 0 01 00 95 0 2 3 2c 55 0 02 00 3c 72 0 01 45 0 00 04 10 11 12 01 00 HH 4 5 6 950 95 1 952
54. onents manufactured under Japanese quality controlling mode BOECO TC PRO Thermal Cycler possess the stable and reliable performance With speedy temperature ramping rate at the maximum of 4 S BOECO TC PRO can adjust ramping rate according to different experimental requirements With multiple temperature control modes the instrument has much stronger compatibility The central processor of BOECO TC PRO Thermal Cycler can automatically distinguish block units as well as run other relevant programs With colourful extra large screen LCD and human oriented operating interface its operation becomes simple and clear It has the function of auto power off protection and continues to complete the unfinished program after the power supply is resumed Height and pressure of hot lid is adjustable so as to fit different reacting tubes on the basis of practical operating situations in order to prevent reagents from the evaporation and pollution RJ45 standard interface can be connected with normal computers thus to achieve easy software upgrading BOECO TC PRO Thermal Cycler Chapter One Introduction 4 Operations of BOECO TC PRO Adopting many advanced techniques blocks of BOECO TC PRO Thermal Cycler can meet different PCR experiments requirements lt holds a leading position in the field of similar products BOECO TC PRO Thermal Cycler Adaptation Carrier BLOCK 96G 96X0 2ml Gradient block Standard Centrifuge Tube 0 2ml BLOCK
55. or on your computer The application may be launched by selecting the installed icons Click Finish to exit Setup 4 Configure your equipment 4 1 Network Connection Link the equipment and computer lines through the network connection Cross Class B BOECO TC PRO Thermal Cycler Connection RJ45 connection Chapter Five Connection Computer Computer 4 2 Network Enable Administrator Search button Configuration Instrument E Network Enablg Configur tion dif COM Magping Monitor Port Monitor IP Address Report Mapping lt v n Configuration 0 Network Enabler Module s Message Log 0 Monitor Lag 0 No Time Description 4 Wow 2008 1 3 14 15 25 ul BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 4 3 Configure Click the Search button the device will search tool automatically N Network Enabler Administrator COM Mapping File Function COM Mapping View Help DOQ SA _ Function COM Mapping 1 COM IP Address COM Port Parameter Configuration NE 41105 192 16 Hi Performance FIFO Ena 9600 None 8 1 Nor dig COM Mapping Monitor Port Monitor Gi IP Address Report Searched equipment IP No Tim Description Double click the searched IP on the column open the attribute page and choice the network option Configuration Information Auto Waming IP Address Report Password Dig
56. puter eq104 gt file run log B Filter lan y gul ss okfile f 8 u2 eq106 User 54 System 125 eg104 8 User huang sg 984 51 s8 5 ag test LA System EE y Setting B Run File StartTime 2007 11 30 15 57 22 2007 11 29 10 15 53 2007 11 29 10 14 17 2007 11 29 10 13 39 2007 11 29 10 12 52 Run 2007 11 29 10 16 31 Complete 2007 11 29 10 15 37 Complete 2007 11 29 10 14 08 Complete 2007 11 29 10 13 27 Complete eq106 eq104 96G gt BOECO TC PRO Thermal Cycler Chapter Five Connection Computer Checking the equipment storage state Double click the System to view equipment storage condition TC PRO User File Tools Help e o Q a ps eq104 gt system state E riter fan ul x B ss Total memory size 51200 eq106 B okfile s Bf Used memory size 3072 mer Free memory size 48128 e E gt User Free memory scan store about 94 54 System Sh eq104 B User Y huang Y 984 B 51 s 5 ag test a BE B Run File Setup the system parameter Double click the setting option of the System options to set up system parameters User File Tools Help e ep e e ae RN TR een RR X OR Sy Se Ub Ox y bal El Ed Computer gul B ss B okfile Bf ju eq106 User Lh System SB eq104 User a huang B RunFile eq104 gt system setting Default Default hotlid temperature OFF 51 Beep alarm when run finish
57. rmal Cycler Chapter Two Specifications lt 0 4C 95C Temp SEXES Uniformity lt 0 3C 72 lt 0 3C 55C el lt 0 3C Accuracy Max Gradient S C 30 C F Min 3 Gradient 1C 1 Set temp range of 30 110 hotlid Dimension 470mmx340mmx260mm LxWxH Weight kg 40 Fuse 250V 8A 5x20 PC Interface RJ45 Note When temperature set value of hot lidis lower than that of block the temperature of hot lid showed in the display would be higher than the set value 4 Performances Maximum number of cycles 99 Maximum number of sections 5 Maximum procedures within a cycle 16 Number of programs 99 Noise 59dB Background Noise below 40dB testing on 1m dead ahead 5 Software Functions File editing and saving File accessing modifying and deleting Adjusting the heating cooling rate automatically Automatic temperature amp time increments decrements during cycling program execution Displaying the data at each phase of program execution instantly BOECO TC PRO Thermal Cycler Chapter Two Specifications Pause of program running Stop of program running Auto restart in case of power failure Soft upgrading via RJ45 interface Sound alarm Estimating total program execution time and remaining program running time Date and time year month day hour minute second display and calibration Failure protection and alarming Note The above software f
58. rs and the characteristics of BOECO TC PRO Thermal Cycler 1 Brief Introduction of PCR Technique PCR Polymerase Chain Reaction or cell free molecule cloning is a technique for amplifying nucleic acids in vitro emulating natural DNA replication process The PCR technique using two DNA strands to be amplified as template and a pair of synthetic oligonucleotides as primers rapidly reproduces the specific DNA pieces under the catalysis of a thermostable DNA polymerase Because of its simplicity rapidity specificity and sensitivity PCR has been widely applied to all fields of life sciences since its invention by Mullis in 1983 and the discovery of the thermostable DNA polymerase by Erlich in 1989 Great achievements have been obtained in PCR s applications in such areas as cytology virology oncology genetics forensics and immunology PCR technology is a milestone in the history of modern molecular biology 2 PCR Applications Research Areas Clinical Diagnoses Immunology Human Genome Project Gene cloning DNA sequencing mutation analysis gene recombination and mutagenesis identification and adjustment of DNA sequence of protein structure detection of gene extension construction of synthetic genes construction of cloning and expression carrier detection of polymorphism of a gene s inscribed enzyme Bacteria spirochaeta rickettsia diphtheria bacillus colon bacillus dysentery bacillus and clostridium Virus
59. the instrument 5 WARRANTY AND SERVICE INFORMATION Please refer to the Warranty Card for the detailed information Note Once it is opened the package should be checked according to the packing list If the buyer finds any items to be missing or damaged do not hesitate to contact the distributor After the acceptance check is passed the buyer should fill out the check form and send its photocopy or fax it to the distributor The Boeckel Co GmbH Co KG establishes the archives and maintenance records with the returned form Please store the package and packing materials in a safe place in case of future device maintenance The above warranty does not extend to goods damaged as the result of cheesy package BOECO TC PRO Thermal Cycler Contents Contents Chapter One Introducton lt lt lt lt HH1 HH kh 1 1 Brief Introduction of PCR Technique aaaaaaaaaasaasaaaaas aaaanaaaassaananannnnnanananannnnndannaaaaaa 1 2PGR Applications EE eM s 1 3 Features ot BOECQCTGO PRO EE 2 4 Operations of BOECO TC PRO AE 3 Chapter Two Specifications AAA 5 1 Normal Working Gohditlons enee eei te 651 5359 414253435140 eei A 5 2 Conditions for Transportation and Giorage enne 5 3 Basic Pa Mei e tenis 5 A perO Ile EA 6 5 Software E Ile e 6 Chapter Three Preparations aaaiaaaaaaaaassasaaaaaanssaaaaaanannnasanaaannnnnnsnanaannnnnnssnaaannnnnnnannnaaaaa 8 1 Structure DescriptiOn 2 32 3 dada adas 8
60. the main interface Press Run key to to enter into file running interface 3 1 How to edit a PCR program File List Interface In the main interface press File to enter into File List Interface There are 3 files BOECO Version 2007 10 A 2007 11 1 10 05 00 listed in the form as showed in the picture When the flashing bar is flashing in the No 2 User BOECO1 BOECO2 File Name Save Time PCR1 PCR2 2003 11 1 2003 11 1 users User Column select through 3 pop up key at this time the File Name Column will display the relative files of the selected user dynamically Shift to the File Name Column where the flashing bar will flash then select relative user s file through pop up key Press Edit to edit the file indicated by the cursor Press New File to edit one new file Press Delete and the system will alarm Confirm delete file then according to the alert information to select Delete or BACK Press Back to go back to the main interface Ifthe current file doesn t have code press Run to enter into File Running Interface and tube type confirmation If the current file has code press Run thus there appears a dialog box Input Password The same as single module Press Back to go back to the main interface Press Accept key to confirm the code If the current Control Mode is block mode press Run runn
61. tlid sensor disconnect sensor broken Contact distributor or manufacturer Ventilation jam Clear the jam Open the device check the 6 ADOBE nange in Ne UNG EE Bad connection connectors for reliable bad temperature control accuracy connection Cooling module failure Contact distributor or manufacturer BOECO TC PRO Thermal Cycler Chapter Seven Failure Analysis and Troubleshooting Significant change in cooling speed or unavailable temperature below ambient one The ambient temperature or humidity is too high which is beyond the normal working condition of the instrument Please regulate the ambient temperature and the humidity Cooling module failure Fan failed or stopped Contact distributor or manufacturer The block cannot be heated or cooled Bad temperature sensor Malfunction of all cooling modules Contact distributor or manufacturer Lid cannot be heated Set the hot lid as OFF in the system parameter setting interface Set the hotlid s state as a certain temperature value The control temperature time is set as in the file edit interface which lead to the hotlid closes automatically Set the temperature control time in digital Loose connection between connectors Lid heater failure Lid sensor failure Contact distributor or manufacturer Abnormal characters displayed in the LCD Bad contact o
62. tual equipment mapping for communications during upgrades Network Enabler Administrator Configuration File Function Configuration View Help Ka geg Function Configuration 0 Network Enabler Module s 2 Network Enabler Admit No Model MAC Address IP Address Status Configuratign E Monitor Port Monitor 9 IP Address Report f configuration Mapping lt T gt Message Log 0 Monitor Log O No Time Description Now 2008 1 3 14 15 25 3 Configuration Click the Search button the device will search tool automatically Network Enabler Administrator COM Mapping File Function COM Mapping View Help DOGOK PES Function COM Mapping 1 COM Za Network Enabler Admir No Model IP Address Pot COM Port Mode Parameter Configuration NE 41105 192 168 0 245 Hi Performance FIFO Ena 9500 None 8 1 Nor dig COM Mapping Monitor Port Monitor XQ IP Address Report searched IP address Message Log 0 Monitor Log 0 No Time Description 35 1 0 1230 1 Configuration E Information Auto Waming IPAddress Repot Password DigitallO SerialCMD Model e S 3 gt Operating Mode NE 41105 Basic Network Seng pero Accessible IPs MAC Address Iv Modify 00 30 E8 10 E2 B7 HE lors OP Mode 00 Real COM Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2
63. un refer to the figure Move cursor by pressing Up Down and Right Left and the position that the cursor reaches will be displayed in white on a black background Press Digit8 Letter to modify parameter settings Press Function to change Press Seg to enter into segment Hotlid off 2007 11 1 10 05 00 1c 95 0 2c 55 0 3c 72 0 4 04 0 01 00 02 00 01 45 00 00 Temp C Time 55 0 72 0 04 0 01 00 02 00 01 45 00 00 Ramp C s HH HH HE Cyclel X10 From 01 to 03 Temp Time Seg Cycle Delete edit interface In every segment the user can set setting temperature Temp duration Time Ramping rate Ramp temperature increment of every cycler Temp and time increment of every cycler Time Press Delete to delete the present segment Press Cycle to enter into segment edit interface where the user can set cycle numbers and initial cycle segment The figure shows a cycle from the first segment to the third one and there are totally 10 times cycles Press Cycle repeatedly to add sections max 5 sections Press Delete to delete the current segment Move cursor by pressing Up Down to enter into the former segment or BOECO TC PRO Thermal Cycler Chapter Four Operation Guide the latter lt Press Save to enter into file save interface Press Back to go back to
64. unctions are for reference only The Boeckel Co GmbH Co KG reserves the right to modify the software functions without notice BOECO TC PRO Thermal Cycler Chapter Three Preparations Chapter Three Preparations This chapter introduces the BOECO TC PRO Thermal Cycler s mechanical structure the keyboard and each button s functions and some preparations before power on Before any operation please read this chapter carefully and make sure you are familiar with the steps introduced 1 Structure Description O Block amp LCD Display amp Logo amp Keyboard Ventilating Hole O USB interface O Sticker of Main body RJ45 interface O Switch 9 Sticker of Block Power Socket Fuse Socket BOECO TC PRO Thermal Cycler Chapter Three Preparations The fuse specifications are as follows 250V 8A 5x20mm The fuse should be replaced by one that meets those specifications If you need certificated fuses please contact the distributor or manufacturer Notes 2 Keyboard Diagram Shift N OOG see N AVE 3 Keyboard Instructions O 2 3 4 6 Digit 8 Letter eee the letter by pressing in 7 8 amp OK 55 0 succession PQRS BOECO TC PRO Thermal Cycler Chapter Three Preparations sur Shift shift from one block to another SE Function change when editing process temperature and modifying time
65. w File menu under File menu pop up the dialog box 3 Imput the file name and password click ok button Create File Do you want to create new file in 1 of Computer Enter new file name The file name can contain only letters and numbers Enter password The password can contain only numbers Enter password again Use empty password ES OK x Cancel Edit file 1 Double click the equipment list user option open the file list and double click edit file or create new file 2 Edit the file content click Save button to save the file Clew May examine the whole steps under the figure When user edit the file if the file has password user must input the password then edit the file BOECO TC PRO Thermal Cycler FA TC PRO Chapter Five Connection Computer 1 ft 5 10 SegD4 720 Zeg 55 00 Seg03_60 0C 01 00 R00 01 00 a Tenp Tine AutoExtionTenp AutoExtionTime Speed Grade 40 10 00 0 0 0 00 Qaa 55 0 50 00 0 0 0 00 Insert 60 0 om 0 0 0 00 Q Delete 72 0 01 00 0 0 0 00 an 01 00 on _Q Copy 55 0 01 00 0 0 B Paste 01 00 0 0 4 0 1000 0 0 roumi _ Down Cycle Begin Segment End Segment Cycle Times Filter all Copy file 1 Choice the file needed to copy 2 Choice the Copy File menu under File menu Paste File 1
66. word through User BOECO File Name Password Password Check pop up key Click left button to delete characters while clicking right button to move the cursor Press Caps to shift the B capital or small letter and there will be a Block X well capital or small letter indication on the left Default User BOECO Default File PCR2 top of the screen As the exam le P Control Mode Block lt Press Main to go back to the main interface Press Save to save the file When the Caps file is normally saved the system will remind File have been saved Please continue another operation Press enter key to cancel the reminding bar If the file was used the system will remind you to rename Rename or continue to save Save Ifthe current Control Mode is Block mode press Run to enter into the file running interface while if the current Control Mode is Tube mode press Run and thus the following dialog Select Sample Volume 0501 I The user can input Sample Volume Unit Micro liter by the same way as that of the main interface Press Back to go back to File List Interface Press Run to enter into the file running interface box will appear Note Setting the password as 0 9 number character BOECO TC PRO Thermal Cycler After the file is running over the system will remind File run over Press Stop and it will
67. y E BOECO Germany THERMAL CYCLER TC PRO Instruction Manual Note The Boeckel Co GmbH Co KG reserves the right to modify this manual at any time without notice Patent materials are included in this manual All rights reserved Any part of this manual shall be forbidden to photocopy reproduce or translate into other languages without written permission from the Boeckel Co GmbH Co KG Please read this manual carefully before the Thermal Cycler is first operated Document Number BYQ60580100000RSM Document Version Mar 2008 Version 1 0 BOECO TC PRO Thermal Cycler Important Note 1 CONVENTIONS Note Because there is important information in this item please read it carefully Failure to follow the advice here will possibly result in damage or malfunctioning of the Thermal Cycler Warning This symbol means that you should be cautious to take an operation procedure Failure to follow the requirements in this item will possibly result in personal injury During the operation maintenance or repair of the BOECO TC PRO Thermal Cycler the following safety measures should be taken Otherwise the guard provided by the Thermal Cycler is likely to be damaged while the rated safety level to be reduced and the rated operation conditions to be affected The Boeckel Co GmbH Co KG shall not be in any way responsible for the consequences resulted from operators not following the requirements Note T

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