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Ettan DIGE Imager - GE Healthcare Life Sciences

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1. 2 Double click the Local Disk C icon Note The C drive may have be named differently than Local Disk t My Computer ie Eide ores TOARE TOK i Gr Oe Aes Bae i F My Computer System Tasks View system information x Add or remove programs G Change a setting Other Places My Network Places My Documents GB Control Panel Ettan DIGE Imager User Manual 11 0036 59 Edition AC 23 2 Installing the Scanner 2 5 Setup the Workstation 24 3 Double click the Windows folder icon Local Disk C File Edit View Favorites Tools Help Hide the contents of this drive F Add or remove programs 2 Search for files or folders File and Folder Tasks gt Ge Move this Folder ID Copy this Folder D Publish this Folder to the Web gt Share this Folder 4 Ifthe following message is displayed click Show the contents of this folder WINDOWS File Edit View Favorites Tools Help Gee QB Ah foes L ve T A A System Tasks Se ort These files are hidden t Folder contents of this F Add or remove programs This folder contains files that keep your system working oP Search for files or Folders properly You should not modify its contents Other Places a Show the contents of this folder s Local Disk C GQ My Documents My Computer My Network Places Ettan DIGE Imager User Manual 11 0036 59 Edition AC Installing the Scanner 2 5 Browse to the Win
2. DIGE Imager is a scanner intended for scanning gels and membranes containing CyDye DIGE Fluor dye labeled proteins or gels post stained with Deep Purple or Sypro Ruby Ettan DIGE Imager should be used with gel cassettes from GE Healthcare The scanner is controlled with Ettan DIGE Imager software which can be set up for a variety of gel formats The software includes a report viewer with functions for image enhancement and distance measuring WARNING Ettan DIGE Imager is intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes The User Manual The Ettan DIGE Imager User Manual is intended for lab personnel performing analysis of fluorescent 2D gels and for service personnel performing maintenance and repairs The user manual comprises the following chapters Important User Information Safety information conventions used in the manual Installing the Scanner Setting up the scanner and the software Getting Started Preparing gel cassettes and setting up a scan Scanning samples Customizing Templates and Gel Formats Using standard and customized gel formats Using the Report Viewer Setting Image Contrast measuring distances and saving modified images File Formats File naming conventions Ettan DIGE Imager User Manual 11 0036 59 Edition AC 3 Preface Maintaining the System Cleaning the scanner and replacing spare parts Troubleshooting Customer verification sheet ch
3. Side of Scanner Power Cable Network Cable Back of Workstation Power Cable Fig 2 5 Power and cable connections WARNING To permit sufficient cooling ensure that the vents on the side panels of the scanner are not covered 7 Plugin the scanner and the workstation 8 Press the Power switch on the side of the scanner and make sure that the POWER light turns on Ettan DIGE Imager User Manual 11 0036 59 Edition AC 17 2 Installing the Scanner 2 2 Set Up the Scanner Power Switch WARNING Do not block the side panel of the scanner The mains power switch must always be easy to access 18 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Installing the Scanner 2 2 3 Setup the Workstation 2 3 1 Setup the Workstation Network Parameters Use the following instructions to configure the workstation so that it can be connected to the scanner To Configure the Workstation Network Interface 1 Turn onthe Workstation and the monitor 2 From the Windows Start menu open the Control Panel 3 Inthe Control Panel click Network and Internet Connections Control Panel File Edit View Favorites Tools Help Ge O Ps Ere Ty Address e Control Panel Va Control Panel A Pick re category GB Switch to Classic view ran bm Appearance and Themes t Printers and Other Hardware lt See Also Windows Update Q Help and Support Network and ot Connections User Accounts o
4. gt Tttan DIGE Images Bat Tips for modifying scan areas To move the scan area hold down the left mouse button on the area and drag the mouse fs war E Metre type Tadaa 26 TEN yi ar Fr Chii Dyr Ei Piim Ge Paim Epia 00 r a Er en w ma mas hme Damaniniai 3 Ge kaa iow ana zr ZE To change the size of the scan area use the mouse to drag an edge or corner of the scan area Ss 2 te eee To delete a scan area select it and Habedsdieccasessearssensevsecerane ress the Delete key i i P i i To specify not to scan an area double click on the area The area is i H aed FRUN rayed out Ee and Fig 4 3 Creating a custom scan area 4 From the Gel Format menu choose Save to save the changes to the new format Save Gel Format 5 In the Save Gel Format dialog box enter a new format name and click OK to save the new format The new Gel format is added to the Gel Format list Ettan DIGE Imager User Manual 11 0036 59 Edition AC 59 4 Customizing Templates and Gel Formats 4 2 Standard and Custom Gel Formats 60 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Using the Report Viewer 5 5 Using the Report Viewer This chapter shows how to use the Report Viewer to document scan data 5 1 About the Report Viewer You can use the Report Viewer to perform quality control checks crop images and to print scanned images This tool allows you to examine the scanned
5. then the resulting output on scan completion will be a filename gel file in the selected directory If two or more scan parameter settings were chosen e g for a Cy2 Cy3 Cy5 gel then the resulting output on scan completion will be a filename ds file in the selected directory and a new directory called filename In this new directory will be a filename ds file and files called UNSEP1 gel UNSEP2 gel and UNSEP3 gel The filename ds file allows the scanned images to be overlaid in ImageQuant whilst the UNSEPn gel files are the individual scan channel outputs and can be viewed as separate files 6 3 Gel file format The images generated by the Ettan DIGE Imager are saved as tiff files However in order to get more information e g instrument settings and comments saved with the image gel files are automatically created Ettan DIGE Imager User Manual 11 0036 59 Edition AC 71 6 File formats 6 3 Gel file format 12 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Maintaining the System 7 7 Maintaining the System This chapter describes procedures required to keep the system hardware in optimum operating condition 7 1 Electrostatic Discharge ESD There is a risk of damaging electronic components with electrostatic discharge when you are performing the maintenance tasks described in this chapter The following guidelines will help you avoid electrostatic discharge Electrostatic discharge is the release of static electricity f
6. See page 36 The following steps do not have to be performed in a strict order 2 Gel format The Gel format list is used to set the scan area In the Gel Format list in the Setup Area choose User Select In this mode you can manually set the area to be Ettan DIGE Imager User Manual 11 0036 59 Edition AC 43 3 Getting Started 3 5 SetUp ascan scanned Then use the mouse and drag a box from the positions of the membrane boundaries The scan area is shown as a blue box in the Grid Area Plan a a a viene lia lon Galen lime anita esha hans omar 6 I oa I I ma a I de I i we I I T3 He I I SS I I ES I I Ae I I TR mie I I ror I I pay 4 ate I I gt I I pra ate I a Ht oe a a ee ee ee ee ee oms ame eee ee eer es ah ee oe cf r am EA tala MS oe o a te alate ia ae z i I I al e I I aj I I 4 I I I I I om of om om of om ee ee ee ee ee ee ee ere ores Peseeeecscuececunauners ee ee ee ee ee ee ee oer I I I I I I I I I I I I I I I I I I I l i Ea a E a a I I I I I I I I I I I I I I I I I I I I ootas tet to pote ate po ttt that tetas ttedas I I I I I I I agm dh ooh bask J t H 4 E E EEE EEE ESE on EEEE EEEE se FEEPEEEEEETHI Fi PEPPER EEEH a i TE EN fe Be ae ee eee Dee ae r i i ate i i OR Ob bt Kb i or or OO i 1 git ct area ek oo rae i job of Rb ob id tot od adaott SAYS ENE ETTA RTR UREA LETAT HRT htt ll
7. default level 1 1 Reset the center of the image to a particular point of interest Open the Image Scaling tool to adjust the brightness and contrast of each channel Select an ROI for cropping the image e RR DD oe Quick Compare a channel with the standard channel This quick comparison is useful for quality checks Show saturated areas in red 3 The Image Area displays one channel of the scanned image Use the tabs at the bottom of the window to view different channels 4 The Report Area at the bottom of the window displays the data for the scan including the user date channel descriptions scan parameters and comments 5 2 Set the Image Contrast You can improve the contrast of selected data in a channel by changing the channel s intensity scale The Report Viewer uses grayscale values to display an intensity scale The lightest grayscale value is mapped to the lowest dimmest intensity value in the wavelength and the darkest grayscale value is mapped to the highest brightest intensity value The remaining grayscale values are mapped to values between the lowest and highest values Grayscale values can be mapped to create linear or nonlinear intensity scales In linear scales the grayscale values are mapped to intensity values that are distributed evenly from the minimum to the maximum values In nonlinear Ettan DIGE Imager User Manual 11 0036 59 Edition AC Using the Report Viewer 5 scales the g
8. e Damage caused by placing apparatus too close to the scanner To avoid injury or damage do not remove the scanner cover Also keep chemicals and equipment that are sensitive to ultraviolet radiation away from the scanner Note The rear cover is equipped with a safety interlock that turns the lamp off Ettan DIGE Imager User Manual 11 0036 59 Edition AC 5 1 Important User Information 1 2 Safety Information if the cover is removed while the lamp is on WARNING Under certain conditions the bulb can explode If this occurs the fumes can be toxic Evacuate the room immediately and remain out of the room for at least 30 minutes 6 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Important User Information 1 1 2 2 Caution and Warning Labels The following labels are found on the product Hazardous Voltage Warning Label WARNING HAZARDOUS VOLTAGE ENCLOSED Hazardous Voltage Enclosed Voltage or current hazard sufficient to Voltage t hazard eufficiant Stipa hocks Mn al cause shock burn or death Disconnect power cond balon servicing Disconnect the system by unplugging the power cord before servicing Heavy Objects Caution Label CAUTION HEAVY OBJECT Heavy Object Can cause strain or back injury Can cause muscie strain or back injwy Use proper lifting techniques when Use proper lifting techniques when moving moving Equipment marked with this label weighs 25 Ibs 11 34 kg or mor
9. images and to rotate and flip them if needed to get the orientation used in DeCuder You can choose to open the Report when a scan is complete or you can open it by clicking Report in the navigation bar Ea Report Viewer Gel File Set 27380 EDI 1 Too View Tools Help 2 Image Controls Gel Format Ettan DALT DIGE File naming format selected Chemistry DIGE min Matrix Type Gel Pixel size 100 0 um Channel Ex Filter tam En Filter inn Exposure Standard CY2 48030 530 40 0 300 l OS 540125 595 25 0 300 Ho Cys Basal E58030 0 300 Mo 4 Report Area Unspecified Sample lO unspecified Individual source unspecified User comment unspecified Wiser name GE Healthcare Prep Date 070a s pise CADataiErikaiScreern shotst2 7380 EDI din27380 EDI ds Fig 5 1 The Report Viewer window has four main areas 1 The Tool Bar across the top of the window is used to open save or print images Ettan DIGE Imager User Manual 11 0036 59 Edition AC 61 5 Using the Report Viewer 5 2 Set the Image Contrast 62 2 The Image Controls on the left side of the window allow you to control the display of the image Use To cr Zoom in on a specific area After you select this tool drag the La mouse across an area to select it Zoom in on the entire image Zoom out on the entire image Return the image to its original center and reset the zoom to the
10. locking cams Bound gels Gels that are cast on Bind Silane treated glass plates remain attached to the treated plate when the top plate is removed Note Make sure that the smaller glass plate of the Ettan DALT is the one treated with Bind Silane 34 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 Gel fixing and staining is performed on the bound gel e g using Deep Purple stain Once the staining procedure has been completed the gel can be placed in the cleaned Ettan DALT SE600 Cassette in two ways 1 Gelside up place in the cassette with the gel side up in the same manner as described for assembled gels Ensure that the glass plate is cleaned and dried before inserting it into the cassette Note To achieve the correct orientation of your image it is assumed that you have bind silane treated the shorter plate and that the strip was placed with the acidic side to the right having the shorter plate towards you when putting on the strip However the image can easily be flipped using the Report Viewer see 5 5 Rotate or Crop the Image 2 Re assembled the untreated plate which was previously removed can be replaced to reform the glass gel glass sandwich To do this a small quantity of the gel storage buffer fix solution needs to be applied to the bound gel and the upper plate carefully lowered onto the gel taking care to exclude air bubbles Alternatively the bound gel can be kept submerged in the
11. panels strips across the scanned area The data for all of the channels in a panel is scanned before moving to the next panel 1 The Status bar displays the channel intensity ranges and saturation values for each panel after it is scanned Saturated areas of the displayed channel are shown in red If the data for any of the channels are saturated or underexposed you can stop the scan and change the scan settings as follows a On the left side of the Scan window click the STOP button Note When you click the STOP button all scanned data is deleted b When prompted click OK to cancel the scan c On the Navigation Bar click Setup d In the Exposure field change the exposure If the scan data is too faint enter a larger exposure If it is overexposed enter a smaller exposure e Click the SCAN button to restart the scan Monitor the scan and adjust exposure values as shown in Steps 2c and 2d above until you are satisfied with the quality of the data and let the scan finish Please see 3 4 1 Finding the optimal settings for a quick way of finding the right settings As the scan is completed a message indicates that the scanner is creating gel files Note When scanning more than one channel scan data files in a gel format are created in the dataset directory Two identical index files in a ds format are also created One index file resides in the dataset directory The other index file resides at the same lev
12. relative humidity less than or equal to 80 non condensing High humidity can result in condensation on the camera window Excessive humidity may also reduce filter life Approximately 140 Ibs 64 kg Pollution POLLUTION DEGREE A in accordance with IEC 664 Space Requirements Workstation 3 ft x 3 ft 90 cm x 90 cm counter space Scanner 2 5 ft x 3 5 ft 77 cm x 107 cm counter space UL 3101 1 First Edition Underwriters Laboratories Inc defines POLLUTION DEGREE 2 as Normally only non conductive pollution occurs Occasionally however a temporary conductivity caused by condensation must be expected 12 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Installing the Scanner 2 Ettan DIGE Imoger e 3 5 ft 107 cm H X Fig 2 2 Scanner counter space requirements 8 GE Healthcore 2 5 ft 77 cm 2 2 2 Minimum Workstation Requirements Graphics card 64Mb with support for 1280x1024 display Operating System Windows XP ver SP2 Network cards 1Gb network card required to connect to the Ettan DIGE Imager Additional network card required if the workstation is connected to a LAN PC Hard Drive Free Space 1Gb minimum 50 Gb recommended if images are stored locally Supports 1280x1024 display Cs WARNING The workstation should be installed and used according to the instructions provided by the manufacturer of the workstation Ettan DIGE Imager User Manual 11 0
13. rotate 70 Ettan DIGE Imager User Manual 11 0036 59 Edition AC 6 Fileformats 6 File formats 6 1 DIGE File Naming Format Using the DIGE File Naming Format option results in all files having user defined and ideally unique filenames All scan images from a given experiment can be saved into a single user defined directory This method of file naming and directory selection results in structures that can be directly used by DeCyder image analysis software If a single scan setting has been chosen e g for a Deep Purple stained gel then the resulting output on scan completion will be a filename gel file in the selected directory If two or more scan parameter settings were chosen e g for a Cy2 Cy3 Cy5 gel then the resulting output on scan completion will be a filename ds file in the selected directory and a new directory called filename dir In this new directory will be a filename ds file and filename suffix gel files were the suffix can be Cy2 Cy3 or Cy5 and the channel that is selected as standard will also have STANDARD as suffix e g Scan1 STANDARD Cuy2 gel The filename ds file allows the scanned images to be overlaid in ImageQuant whilst the filename suffix gel files are the individual scan channel outputs and can be viewed as separate files 6 2 Standard File Naming Format The DIGE File Naming Format check box is left unselected in this mode If a single scan setting has been chosen e g for a Deep Purple stained gel
14. the clips securely attach the bulb to the plate e Connect the lamp cable to the new assembly The cable connectors are keyed so that they only connect in the correct position e Install the lamp assembly Be careful not to pinch the cables e Hold the lamp assembly plate flush against the scanner frame and finger tighten the two lamp assembly thumb screws clockwise CAUTION Do not use a screwdriver to tighten the assembly plate screws e Close the lamp access door and install the rear panel 3 Reset the bulb age counter as follows e Start the system e Open the Ettan DIGE Imager Scan window and choose Instrument Reset Lamp Age e When prompted click OK to set the lamp age to zero 4 Perform a flat field calibration as follows e Place the special calibration paper in a cassette and then place the cassette in the scanner e From the Ettan DIGE Imager window menu choose Instrument Flat Field Calibration e When prompted click OK to calibrate the scanner 80 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Troubleshooting 8 8 Troubleshooting 8 1 Customer verification test target Enclosed with the instrument is an instrument verification test target It can be used to check that the instrument is working properly Simply place the target on the glass plate of the sample cassette so that the numbers appear with normal orientation prevent the target from moving by securing it with two pieces of tape and scan the wh
15. the system 54 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Customizing Templates and Gel Formats 4 4 Customizing Templates and Gel Formats This chapter shows how to create and modify a template and how to set up a custom format to scan gels 4 1 Templates You can create templates that save the gel formats and scanner parameters for ascan When you load a similar type of sample in the cassette you can open the template that loads these scan settings This is useful when you are scanning a number of samples that have similar attributes 4 1 1 Create a New Template You can create a new template after you set up a scan If you have already created a template that is similar to what you need you can load it modify it and save it with a different name using Save as To create a template from a scan 1 Setup a scan as shown in 3 3 Set Up a scan 2 Inthe File menu choose Save Template As Then enter the name for the new template in the Scan Template Name field and click OK Save Template As 2 Scan Template Namie E j ryneyy Template The new template is saved in your user preferences When you are logged on to your account it appears in the Open Scan template dialog box The template is not available to other users To create a template from an existing template 1 Inthe Ettan DIGE Imager window choose File Open Template and select a template in the Open scan template dialog box Ettan DIGE Imager User
16. up on the panel as you open it and remove the door Fig 7 3 Removing the rear pane e Open the lamp Access door Ettan DIGE Imager User Manual 11 0036 59 Edition AC 1 7 Maintaining the System 7 4 Replace the Lamp Assembly Lamp Access Door Fig 7 4 The Lamp Access door e Remove the two lamp assembly thumb screws at the lower left and upper right hand corners of the lamp base CAUTION If the plate is hot wait for it to cool before unscrewing the lamp assembly screws Lamp As sembly Screws Plastic Knob Fig 7 5 Lamp assembly screws and plastic knob e Grasp the plastic knob and gently pull out the lamp assembly 78 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Maintaining the System 7 WARNING DO NOT TOUCH THE BULB Fingerprints on the bulb can cause it to overheat when it is turned on and possibly explode If this occurs evacuate the room immediately and remain out of the room for at least 30 minutes Order a replacement bulb assembly e Disconnect the lamp cable from the assembly and put the assembly aside Lamp Cable Connector Fig 7 6 Lamp Cable Connector and Assembly e Remove the new lamp assembly from its packaging Note Use only replacement bulbs supplied for this system Ettan DIGE Imager User Manual 11 0036 59 Edition AC 19 7 Maintaining the System 7 4 Replace the Lamp Assembly e Being careful not to touch either the front or back of the bulb make sure that
17. with permanently mounted glass plate Mounted Glass Cassette should be used In order to achieve optimal calibration when scanning the black plastic hold down plate should be placed on top of the membranes Note Wear powder free gloves when handling the cassette and membranes The powder used in laboratory gloves can fluoresce and may also scatter light affecting image quality To load a cassette 1 Clean the glass plate with distilled water and dry it using a lint free cloth Since fluorescent material has come into direct contact with the glass plate we recommend using a lint free tissue moistened with 10 hydrogen peroxide to remove this material followed by cleaning with distilled water 2 Place the membranels directly on the plate with the protein side facing down If the membrane s is wet make sure to exclude air bubbles when positioning it 3 Note the positions of the membrane boundaries on the plate You will use this information to determine the areals to be scanned see Set Up a scan 4 Place the hold down plate in the cassette on top of the membranes Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 5 Put the lid on the cassette see Fig 3 4 and close the cassette by turning the locking cams up 3 2 3 Inserting a cassette To insert the cassette 1 Slide the Scanner door at the top of the scanner until it is fully open Fig 3 6 The Scanner door 2 Insert the cassette into the scanner
18. 036 59 Edition AC 13 2 Installing the Scanner 2 2 Set Up the Scanner 14 2 2 3 Install the Scanner Unpack and install the scanner as shown below To install the Scanner 1 Remove the scanner from the crate and place it on a flat level surface WARNING Do not attempt to lift the scanner by yourself Two people are required to lift the scanner 2 Slide the door fully open to get access to the stage area Fig 2 3 Opening the scanner door 3 Inthe upper left corner of the stage area use a hex key to loosen the locking bolt that holds the stage in place and remove the locking bolt Ettan DIGE Imager User Manual 11 0036 59 Edition AC Installing the Scanner 2 Fig 2 4 Removing the locking bolt 4 Remove the locking bolt and the round metal plate CAUTION Do not discard the locking bolt and the plate You will need them If you ever have to move the scanner 5 Store the locking bolt and the plate as follows 1 Remove the rear panel by loosening the screws at the bottom of the panel Ettan DIGE Imager User Manual 11 0036 59 Edition AC 15 2 Installing the Scanner 2 2 Set Up the Scanner 2 Gently pull up on the panel as you open it to remove it 3 Open the optics bay door next to the lamp access door Optics Bay Door 5 Reassemble the rear panel 16 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Installing the Scanner 2 6 Connect the scanner cable to the workstation as follows
19. 11 0036 59 Edition AC 29 3 Getting Started 3 1 Turn the System On and Off jScanner Lights Power Switch Fig 3 1 Scanner power switch and lights 4 After the SCANNING light turns off double click the Ettan DIGE icon on the desktop to start the DIGE software 30 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 e If the Scanner not responding message is displayed click Yes Scanner not responding Q Connection to the Ettan DIGE Imager is failing Reason Could not create communication link to DIGEImager The scanner may OFF or not fully initialized Keep trying e If the monitor displays the Connection Failed message make sure that the network cable is connected properly and that the SCANNING light is off Then double click the Ettan DIGE icon on the desktop Connection Failed x Could not connect to the Ettan DIGE Imager Verify that the scanner is turned On and the communication cable is installed properly and restart this application 5 When the workstation connects to the scanner the Ettan DIGE Window opens Ettan DIGE Imager User Manual 11 0036 59 Edition AC 31 3 Getting Started 3 2 Prepare the Cassette for a scan 32 3 2 Prepare the Cassette for a scan Before scanning a sample you must insert it in a cassette Cassettes are designed to hold and store gels or membranes during scanning They are chemically resistant and can withstand common ge
20. E Imager is Failing Reason Could not create communication link to DISEImager The scanner may OFF or nok Fully initialized Keep trying Yes er 2 Installing the Scanner 2 5 Setup the Workstation If the monitor displays the Connection Failed message make sure that the network cable is connected properly and that the scanner SCANNING light is off Connection Failed x Could nok connect to the Ettan DIGE Imager verify that the scanner is turned On and the communication cable is installed properly and restart this application When the workstation connects to the scanner the Ettan DIGE Imager Window opens 28 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 3 Getting Started This chapter shows how to set up and run a scan and print a scan report 3 1 Turn the System On and Off To turn on the system 1 Make sure that the scanner door is closed 2 Turn on the scanner and the workstation When you turn on the scanner the POWER light turns on 3 The yellow SCANNING light turns on as the scanner initializes When the scanner completes its initialization the SCANNING light turns off The Scanner has two other status indicators e The SCANNING light turns on when the system is acquiring data e The READY light turns on when the system is ready for scanning the lamp is on and the camera has cooled to the required operating temperature Ettan DIGE Imager User Manual
21. Exposure Standard 1 CY2 480 30 53040 0 300 H 2 i 540125 545 25 0 300 Ho 3 oya 63530 68030 0 300 ho el ID Unspecified Sample IE unspecified Individual source unspecified User comment unspecified Wiser name GE Healthcare Prep Date OFf03 5 File CDatalcrikalscreen shotst27330_EDl direr3380_EDI ds Fig 3 21 The Report Viewer The Report Viewer summarizes the View area and the Information Summary area of the Scan View Use the Report Viewer to view an image of each channel along with the scan settings and comments associated with the scan data You can use the Report Viewer to reorient or crop the scan data You can also use it to adjust brightness and contrast of the display measure features and add a scale bar See 5 Using the Report Viewer To print reports e To print Scan reports for all of the dyes choose File Print All in the Report Viewer menu Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 To pn ascan report for a specific dye select the Dye tab cvs cvs in the Report viewer Then choose File Print Current in vine Report Viewer menu 3 6 To turn off the system 1 Save all data files 2 Inthe Ettan DIGE window choose File Exit to close the scanner software 3 Shut down the workstation 4 Turn the Power switch off to shut down the scanner Ettan DIGE Imager User Manual 11 0036 59 Edition AC 53 3 Getting Started 3 6 To turn off
22. GE Healthcare Ettan DIGE Imager User Manual Important user information All users must read this entire manual to fully understand the safe use of Ettan DIGE Imager IMPORTANT Ettan Dige Imager is intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes WARNING The WARNING sign highlights instructions that must be followed to avoid personal injury Do not proceed until all stated conditions are clearly understood and met CAUTION The CAUTION sign highlights instructions that must be followed to avoid damage to the product or other equipment Do not proceed until all stated conditions are met and clearly understood Notes Note A Note is used to indicate information that is important for trouble free and optimal use of the product Recycling This symbol indicates that the waste of X electrical and electronic equipment must not be disposed as unsorted municipal waste and mm Must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment WARNING This is a Class A product In a domestic environment it might cause radio interference in which case the user might be required to take appropriate measures WARNING All repairs should be done by personnel authorized by GE Healthcare Do not open any covers or replace any parts unless specifically stated in the instructio
23. MUM Workstation Requirements mmm 13 2 2 3 HOS GUT FOO T oa E E E EROSION 14 23 Setupihe WORKS tatO emesis 19 Pad Set up the Workstation Network Parameters 19 2 3 2 Install the Scanner software sms 27 Pos WEEE Me TS COM OUOG sroine ani EAEE 27 3 Getting Started 3 1 Turn the SYSteM ON AN OFF musmmsmesssmmsssspsussnsuarosssin 29 3 2 Prepare the Cassette for a SCAN wicccccsecsssesssecssecssessseesseesseesseesseen 32 32l TCS OF C CEE uaa ETR EER 52 3 20 Loading COS SEG ansaa a AN ENN 55 32 5 Inserting O CO SSE sassresssssedsnsnsasessnrsorsinsnssin anaren SF 53 ACUP CT e E 39 3al E E AOI PR AAA EAE 40 3 3 2 Scanning MOEMONONECS sociis ninani enan EnA 45 3A STON a OMD oeae isens 45 3 4 1 PHONG the opumnmal setin E asriar 45 3 4 2 ib 0 EE EE 8 E TANEN T Te ern A fr entree A O er omer NR 4 39 TAEREPO VIEW aaecceeresnteexctesincccre coronene em nr 52 3 6 Totara OM CTS SF srci 53 4 Customizing Templates and Gel Formats MTS Sse cece tesscezaaceaasceeevepavevsececiencerearecesuscensste sarannccteerusteevoricshctpeees 55 4 1 1 Create a New Template s s s sisssissssisiisistissisnssssessosssnsiasessniessisrsisssnnssstnn 55 4 2 Standard and Custom Gel Formats mmmm ssoebmmsnssssssnmnn 56 421 Standard Gel Formals sssrin a NR 56 4 2 2 Create Custom Gel FON O CS escaire 57 5 Using the Report Viewer 5 1 About the Report Viewer missen 61 52 SELIN 16 0 218 CONnTOSt medisini OTe 62 53 DISDIGU ithe lmage Scale BO srssssmseea 65 E
24. Manual 11 0036 59 Edition AC 55 4 Customizing Templates and Gel Formats 4 2 Standard and Custom Gel Formats Open scan templat Eg my Template heyy Template Read only template 2 Inthe Setup area modify the settings for the Gel Format Chemistry and other parameters and select the dyes for the new template 3 In the File menu choose Save Template As Then enter the name for the new template in the Scan Template Name field and click OK 4 2 Standard and Custom Gel Formats The gel format controls the shape of the scan area If you are using a DALT gel you can use the standard DALT gel format Ettan DALT You can also use standard formats for any other gels provided that these formats scan all of the areas covered by the gels CAUTION When setting up a gel format make sure that the scan area includes all of the area covered by the gel Because the gels have irregular shapes some areas outside of the gel perimeter should be scanned 4 2 1 Standard Gel Formats The Gel Format list includes the following standard Gel formats These formats are read only and cannot be modified 56 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Customizing Templates and Gel Formats 4 Uneditable Gel Format qmoauwuvor 7Yt4f T H H i ji H H y H ot 0 H H y H in y H H y H ab H H H H H H H i H h H H H H nt 0 i i Zoagrnrrda gt sx
25. Properties Description L Transmission Control Protocol lnternet Protocol The default Wide area network protocol that provides communication across diverse interconnected networks C Show icon in notification area when connected Notify me when this connection has limited or no connectivity 7 Inthe Internet Protocol TCP IP Properties dialog box enter the address as follows 1 Select Use the following IP address 2 In the IP address field enter 159 159 159 2 3 In the Subnet mask field enter 255 255 0 0 4 Leave the remaining fields blank 5 Click OK Internet Protocol TCP IP Properties General You can get IP settings assigned automatically if your network supports this capability Otherwise you need to ask your network administrator for the appropriate IP settings Obtain an IP address automatically Use the following IP address IP address 159 159 159 2 Subnet mask 255 255 gd 0 Default gateway Obtain DNS server address automatically Use the following DNS server addresses Preferred DNS server Alternate DNS server Ettan DIGE Imager User Manual 11 0036 59 Edition AC 21 2 Installing the Scanner 2 3 Setup the Workstation 8 Close the Local Area Connection 2 Properties dialog box 4 Local Area Connection 2 Properties 2 x General Authentication Advanced Connect using B Broadcom NetXtreme Gigabit Ethem
26. Standard E 54025 59525 2 63550 68050 3 CrS BSS B200 e x a wl Fig 3 10 Parameters and settings in the Setup Area The steps below do not have to be performed in a strict order 1 Matrix type Choose Gel in the Matrix type drop down list 2 Gel format The Gel format list is used to set the scan area There are three options for setting the scan area Option 1 Predefined Gel Formats For Ettan DALT SE600 gel and for one to six naked miniVE gels there are predefined gel formats see section 4 2 Standard and Custom Gel Formats Option 2 User Select In this mode you can manually set the area to be scanned e g can be used when scanning two naked minVE gels In the Gel Format list in the Setup Area choose User Select Then use the mouse and drag a box Option 3 User defined gel format custom gel format You can use a predefined custom created gel format from the Gel Format Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 list in the Setup Area See section 4 2 2 Create Custom Gel Formats for information about how to create custom gel formats Select the appropriate gel format for your sample The scan area is shown as a blue box in the Grid Area Fig 3 11 Grid Area 3 Chemistry Specify the type of chemistry used for your sample by selecting an item in the Chemistry list 4 Pixel Size Select the pixel size i e resolution to use in the Pixel size list 100 um should b
27. addresses to host names Each a should be kept on an individual line The IP address should be placed in the first column followed a4 the corresponding host name The IP address and the host name should be space separated by at least one ey patna comments Csuch as these may be inserted on individual lines or following the machine name denoted by a symbol For example Se Fe Fe Fe te H H H H H H H H H H H H all 102 54 94 97 rhino acme com source server 38 25 63 10 x acme com x client host 127 0 0 1 localhost 159 159 159 1 DIGEImager 9 From the File menu click Save 10 Close the Notepad window Ettan DIGE Imager User Manual 11 0036 59 Edition AC Ettan DIGE Imager User Manual 11 0036 59 Edition AC Installing the Scanner 2 2 3 2 Install the Scanner software Use the CD with the auto run script to install the software as follows IMPORTANT Study the readme file thoroughly To verify that the workstation can connect to the scanner 1 Start the workstation and place the CD in the CD ROM 2 Follow the instructions on the screen to install the software 2 3 3 Verify the installation To verify that the workstation can connect to the scanner 1 Make sure that the Scanner SCANNING light is off 2 Double click the Ettan DIGE icon to start the workstation software 3 Ifthe Scanner Not Responding dialog is displayed click Yes to keep trying Scanner not responding Q Connection to the Ettan DIS
28. and wait until the lamp warms up The Scanner Lamp Warming Up Message box displays an estimated time of when the scanner will be ready and closes when the lamp is warmed up this takes approximately 5 minutes When the Scanner Lamp is warm the READY light is on 3 Click the SCAN button to start the scan 4 Inthe File Name Dataset dialog box browse to a directory and enter a name for the dataset or choose New folder to create a new folder Ettan DIGE Imager User Manual 11 0036 59 Edition AC 47 3 Getting Started 3 4 Scan a sample File Name Gatasel Desktop GE Healthcare EE Ahly Computer SEUPPD I0465 H sci i My Documents amp 4 My Network Places Dataset Name DIGE gel 2563 Co Fig 3 18 File Name Dataset dialog box Note Depending on whether the DIGE File Naming Format check box has been selected or not the scanned files will end up with different filenames and folder structure see 6 File formats for more information on DIGE File Naming Format Note Dataset names must be unique If you enter the name of an existing dataset you will be prompted to overwrite it 5 Click Proceed to start the scan After you press the SCAN button the view changes to Scan and the bottom label changes to STOP 48 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 5 The View Area gt Ettan DIGE Imager EJE x File Edit GelFormat Result Instrument Help ra gy Lid I informati
29. as follows Insert the cassette with the cams towards you and tilted up so that the cassette fits under the beveled edge on the carrier Ettan DIGE Imager User Manual 11 0036 59 Edition AC 37 3 Getting Started 3 2 Prepare the Cassette for a scan Fig 3 7 Inserting the cassette Push down and in on the front of the cassette to lock it in place Fig 3 8 Locking the cassette 3 Close the scanner door 38 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 3 3 Set Up a scan Start the Scanner Software by double clicking the Ettan DIGE Imager icon Gon the workstation desktop or by using the start menu Start Programs GE Healthcare Ettan DIGE Imager The scanner software Ettan DIGE Imager window opens in Setup View 4 The Setup Area Choose matrix type here 1 The Tool Bar Ettan DIGE Imager EE File Edit GelFormat Result Instrument Help DEE D pg er Information F 2 The a Instrument status User Select Pixel size zho Unassigned Matrix type Gel v DIGE File Naming Format SCAN Channel Dye Ex Filter nm Em Fitter nm Exposure Standard Filter Details V4 480 30 530 40 0 400 v Estimated Scan time min sec a CY3 540 25 595 25 0 200 Estimated Disk Space Needed MB 00 3 CYS 635 30 680 30 0 200 Total Disk Space Available MB 12688 66 0 p200 v oOo 4 C 540 25 595 25 0 200 eriment Comments i a ee e
30. asures the distance between two points Measurement starts over after the second point Single Reference measures distances from a single reference point The first point serves as the reference point The distance is measured between this reference point and subsequent points Ettan DIGE Imager User Manual 11 0036 59 Edition AC Using the Report Viewer 5 Leap Frog measures the distance between two points The end point of each measurement serves as the start point of the subsequent measurement Multiple Segment measures the distance between a series of points 3 Select which unit to use for measurement in the Distance Units list 4 To display lines between points select Draw Lines 5 To display point labels on the image select Label Points 6 Use the mouse to select two points in the Image window Fig 5 8 Selecting points to measure 7 Inthe following example Line 1 consists of Point 1 and 2 and is 362 365 millimeters long Ettan DIGE Imager User Manual 11 0036 59 Edition AC 67 5 Using the Report Viewer 5 5 Rotate or Crop the Image 68 Measure Distances x Draw Lines Label Points Measure Method Sent E aE Point RA Distance Units Line Point x ve Distance Total 1 1066 792 A 1 2 760 598 362 315 362 315 Delete One Line Delete All Lines Export Data 8 When you are finished click Export Data to save the list as a csv file This format can be opened in a spreadsheet 9 View the results of m
31. d This setting blocks all outside sources from connecting to this computer with the exception of those selected on the Exceptions tab C Don t allow exceptions Select this when you connect to public networks in less secure locations such as airports You will not be notified when Windows Firewall blocks programs Selections on the Exceptions tab will be ignored x Off not recommended Avoid using this setting Turning off Windows Firewall may make this computer more vulnerable to viruses and intruders Windows Firewall is using your domain settings What else should know about Windows Firewall 22 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Installing the Scanner 2 11 Verify the Local Area Connection 2 box is not checked and click OK Windows Firewall General Exceptions Advanced Network Connection Settings Windows Firewall is enabled for the connections selected below To add exceptions for an individual connection select it and then click Settings M Local Area Connection E Local Area Connection 2 Security Logging You can create a log file for troubleshooting purposes ICMP With Internet Control Message Protocol ICMP the computers on a network can share error and status information 12 Close the Network Connections window To Map the Scanner Address 1 Double click the My Computer desktop icon oe et li nd My Computer P Internet Explorer
32. dows system32 drivers etc folder and double click the hosts file icon KB s 2 nosts Imhosts File and Folder Tasks File zaal SAM File B 4 Gi Rename this fle Mowe this file IC Cony this file K Publish this file to the Web Email this file x Delete this file Other Places D divers 6 Ifthe following message is displayed click Select the program from a list and click OK File Edit View Favorites Tools Help File and Folder Tasks v t program crez fe WS U can manually selec ist of ype File Date Modifi 7 00 AM Size 734 bytes Ettan DIGE Imager User Manual 11 0036 59 Edition AC 25 2 Installing the Scanner 2 3 Setup the Workstation 26 7 Inthe Open With dialog box select Notepad and click OK File Edit View Fayortes Tools Help Om OB Paws iene l File and Folder Tasks i Rename this file Ge Move this file TD Copy this file K Publish this file to the Web E E mail this File XK Delete this file H ImageQuant Application Other Places 4 a Internet Explorer B drivers My Documents s9 My Computer amp Microsoft Photo Editor E My Network Places Microsoft PowerPoint Microsoft Word 8 Inthe Notepad text editor append the following line to the bottom of the text file 159 159 159 1 DIGEImager hosts Notepad copyright c 1993 1999 microsoft corp This is a sample HOSTS file used by Microsoft TCP IP for windows This file contains the mappings of IP
33. e Fig 5 6 The Scale Bar dialog box 2 Select Displayed to show the Scale Bar Ettan DIGE Imager User Manual 11 0036 59 Edition AC 65 5 Using the Report Viewer 5 4 Measure Distances 66 3 Todisplay the scale as a vertical bar select Vertical the default is horizontal 4 Adjust the Position Length Thickness and Color of the scale bar The scale bar changes interactively as you set these properties Note To specify length you can enter a value in mm in the Length field or use the slider 5 To hide the scale bar un select the Displayed option 5 4 Measure Distances Use the Measure Distance tool to measure the distance between two points the distance from a single reference point to a series of points or the distance between a series of points After you complete your measurements you can export them as a comma delimited list to a file that can be opened in a spreadsheet To measure distance 1 In the Report Viewer window choose Tools Measure Distance to open the Measure Distances dialog box Measure Distances x Draw Lines Label Points O Measure Method Standard Two Point Vv Distance Units Millimeters Vv Line Point x we Distance Total Delete One Line Delete All Lines Export Data Fig 5 7 The Measure Distance tool 2 Select the Standard Two Point method in the Measurement Method list The Measurement Method list has the following options Standard Two Point me
34. e This connection uses the following items Client for Microsoft Networks r File and Printer Sharing for Microsoft Networks JE QoS Packet Scheduler 3 Internet Protocol TCP IP Description Allows your computer to access resources on a Microsoft network C Show icon in notification area when connected Notify me when this connection has limited or no connectivity m cae 9 Inthe Network Connections window select Local Area Connection 2 and click Change Windows Firewall Settings 3 Network Connections File Edit View Favorites Tools Advanced Help Q O P ah HE Folders Address Network Connections A Name Network Tasks LAN or High Speed Internet CE ee ek Local Area Connection LAN or High Speed Inter Connected Firewalled Local Area Connection 2 LAN or High Speed Inter Connected Firewalled Change Windows Firewall settings amp Disable this vend device a Repair this connection m Rename this connection view status of this connection Change settings of this connection Other Places g Control Panel 10 In the Windows Firewall window click the Advanced tab Windows Firewall General Exceptions Advanced Windows Firewall is helping to protect your PC Windows Firewall helps protect your computer by preventing unauthorized users from gaining access to your computer through the Internet or a network Y On recommende
35. e a Sr as aes ey AS RT Pa aa a ae a a 3 The Information Area Se ST Tea Galen KT React SR Siem Hae iment Titans Fame Silvery apm a San Gms aaa hace ee avast Holme eae eat me ies Ea iy et a SR RN S VOLS Gage Meet LA Kos LT eh Ne mn seek el fe ES Pr SAS Hees re fees mace fen fete aur CE et Gees fee eam Fe teas FAS ee ee a 12345 G6 7 8 9 101112131415 161718192021 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Cassette is not loaded or cassette door is open 5 The Grid Area Fig 3 9 The Ettan DIGE Imager Setup View 1 The Tool Bar is used to manage scan templates and to turn on the scanner light 2 The Navigation Bar shows the status of the scan workflow Ettan DIGE Imager User Manual 11 0036 59 Edition AC 39 3 Getting Started 3 5 SetUpascan 40 3 The Information Area shows the instrument status displays information about the scan and includes the SCAN button that is used to start a scan 4 The Setup Area contains the parameters and settings for the scan 5 The Grid Area defines the area or areas to scan The grid coordinates are aligned with those on the cassette 3 3 1 Scanning gels Set up a gel scan In the Setup Area of the setup view the parameters and settings for the scan are set Setup Gel Format User Select Pixel size um a Chemistry Matrix type Cl cel MD DIGE File Naming Format en Channel Dye Ex Fiter nim Em Fiter nm Exposure
36. e and should be lifted by two people Hot Surfaces Caution Label HOT SURFACES Do not touch Do not louch Allow surfaces to Allow surfaces to cool prior to cool prior to servicing Servicing Fig 1 1 Description of Caution and Warning Labels Ettan DIGE Imager User Manual 11 0036 59 Edition AC i 1 Important User Information 1 2 Safety Information Waste of electrical and electronic equipment This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment Fig 1 2 Recycle symbol 8 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Important User Information 1 The following safety labels are found on the back of the product Access door at back of scanner ee aa a Heavy Object Can cause muscle strain or back injury Use proper lifting techniques when moving Back of scanner with Access door removed Hazardous Voltage HOT SURFACES Enclosed Do not touch Voltage or curent hazard sufficient Allow surfaces to l asa Shock burn or d alh cool prior to servicing Disconnect power cond baloni servicing Fig 1 3 Location of Caution and Warning labels 1 3 CE Certification This product meets the requirements of all applicable CE directives A copy o
37. e used if images are to be analyzed by DeCuder 5 Channels and dyes Select the number of channels to be scanned by clicking the Channel check box Between one and four channels can be programmed Selection of the Channel check boxes must be performed in sequential numerical order For each selected channel select a dye to be scanned in that channel When selecting a dye the excitation and emission filters that are used with that dye are automatically displayed by the software The excitation and emission filter combinations have been selected to deliver optimum results with a minimum of crosstalk Channel Dye Ex Fitertnm Em Fitertnr Exposure Standard 1 540 25 595 25 Cle lev 540 25 595 25 d 635 30 660 30 mn 40 4 Deep Purple 1 541 25 595 25 User Mame GE Healthcare Experiment Comments Fig 3 12 Filter settings Ettan DIGE Imager User Manual 11 0036 59 Edition AC 41 3 Getting Started 3 5 SetUpascan Note There are two filter combinations for Deep Purple and Sypro Ruby available as Deep Purple Sypro Ruby 1 and Deep Purple Sypro Ruby 2 Deep Purple 1 and Sypro Ruby 1 should be used for optimum results whereas Deep Purple 2 and Sypro Ruby 2 are optional filters to be used in order to minimize crosstalk i e when Cy dyes are present in the gel If you have selected DIGE file naming format and have a standard defined in your experiment specify which dye represents the standard under Standard 6 Exposure S
38. easurements in the Point List table 5 5 Rotate or Crop the Image You can rotate or crop the image in the Report Viewer This can be useful for interpreting or presenting the data 5 5 1 Rotate the Image You can use several methods to reorient the image To Reorient the Gel Image e In the Edit menu choose one of the following Items Flip X flips the image horizontally Flip Y flips the image vertically Rotate 90 rotates the image 90 clockwise Invert XY switches the X and Y axis and reverses the orientation of the axes 5 5 2 Crop the Image Crop the image to include only the areas that include data To Crop the Image 1 On the Report Viewer tool window select the ROI LJ Region of Interest tool 2 Drag the mouse across the area of interest to define the ROI The ROI is displayed as a square Ettan DIGE Imager User Manual 11 0036 59 Edition AC Using the Report Viewer 5 Report Viewer Gel File Set 27380 EDI File Edit wiew Tools Help TE G I Sel Q Q fat be H E cy2 CY3 cvs Gel Format Ettan DALT DIGE File naming format selected Chemistry DIGE min Matrix Type Gel Pixel size 100 0 um Channel Dye Ex Filter trim En Filtertnid Exposure Standard Cy 480 30 53040 0 900 ho i 540 25 595 25 0 300 Mo CYS Basran papag 0 300 Mo Gel ID Unspecified Sample ID unspecified Individual sou
39. eck that the instrument is working properly Appendix Filter details related products consumables and spare parts 4 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Important User Information 1 1 Important User Information 1 1 Warnings and Cautions Hazardous conditions are indicated in the User Manual by Warning and Caution messages WARNING The Warning sign highlights an instruction that must be strictly followed in order to avoid personal injury Do not proceed until you clearly understand the instructions and all stated conditions are met CAUTION Indicates important information regarding potential damage to equipment or software Follow the instructions in detail in order to avoid damage to the instrument or other equipment Do not proceed until you clearly understand the instructions and all stated conditions are met 1 2 Safety Information The Ettan DIGE Imager system is equipped with safety labels and interlocks to help protect you from potential injury that may be caused by ultraviolet light heat or the high voltage of the scanner s lamp 1 2 1 Ultraviolet Light and Heat The following hazards are associated with the scanner lamp assembly s high intensity metal halide bulb e Damage to eyes and skin caused by exposure to ultraviolet radiation e Burns caused by contact with a hot lamp assembly e Fire ignited by hot lamp assembly e Interaction of nearby chemicals with ultraviolet radiation
40. el as the dataset directory When scanning one channel only scan data files in a gel format are created When the scan is complete the Scan Finished dialog box appears Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started Scan Finished The Scan has successfully finished Selected Scan Area Channel 1 CY2 at 0 90 s exposure Signal Min Max Mean 0 65535 1841 27 Channel 2 CY3 at 0 35 s exposure Signal Min Max Mean 0 65535 2950 98 Channel 3 CYS at 0 70 s exposure Signal Min Max Mean 0765535 616 15 View Report Return to Setup Fig 3 20 Scan finished dialog box 5 To close the Scan Finished dialog box and return to the Setup View click Return to Setup 6 Toview a report choose a scan area in the Select Scan Area list and click View Report Ettan DIGE Imager User Manual 11 0036 59 Edition AC 51 3 Getting Started 3 5 The Report view De 3 5 The Report view gt Report Viewer Gel File Set 27380_EDI EJ File Edit wiew Tools Help lar A Q pasgi T h Q oe m ro ie j Ta cv teer I SPE a e 44 t LJ i i a al be a i t a ve 4 Tiig ai y y Fe a r a EN Lod t i oa o ee EO E l n l cv 2 CY3 cys Gel Format Ettan DALT DIGE File naming format selected Chemistry DIGE_min Matrix Type Gel Pixel size 100 0 um Channel Dye Ex Filter im En Filterinmi
41. elect the exposure to be used for each channel in the Exposure list A quick test scan on a small area should be performed initially to identify a suitable exposure See section 3 4 1 Finding the optimal settings 7 Comments You can optionally add comments to your data To add comments to the data 1 At the bottom of the Setup Area click Experiment Comments to open the Experiment Comments dialog box Experiment Comments Gel ID Use this Comment f r all Gels 2 Enter the Gel ID the Individual Source and the Sample ID 3 Enter Comments for the scan To use the same comments for all the gels in this scan select Use this Comment for all Gels This option only applies to the Comments field All other fields are always specific to each gel area 4 Click Close 42 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 3 3 2 Scanning membranes Set up a membrane scan In the Setup Area of the setup view the parameters and settings for the scan are set Setup Gel Format User Select Pixel size um See Chemistry Matrix type C Membrane DIGE File Naming Format Channel Dye Ex Fiter nim Em Fiter nm Exposure Standard 4 E 540 25 595 25 2 BE v 635 30 680 30 13 635 30 680 20 D4 Fig 3 13 Parameters and settings in the Setup Area 1 Matrix type Choose Membrane from the Matrix Type list Note When scanning membranes the black plastic hold down plate should be used
42. f the corresponding Declaration of Conformity is available on request Ettan DIGE Imager User Manual 11 0036 59 Edition AC 1 Important User Information 1 3 CE Certification 10 Ettan DIGE Imager User Manual 11 0036 59 Edition AC 2 Installing the Scanner 2 Installing the Scanner This chapter describes the installation of the Ettan DIGE Imager the scanner It also describes components and applicable requirements 2 1 About the Scanner The scanner allows you to scan a variety of gel formats Fig 2 1 The scanner The scanner has the following replaceable parts Component Part Number Lamp Assembly 28401403 Protective Window 28401414 2 2 Set Up the Scanner Before you set up the scanner review the installation requirements Then use the following instructions to unpack the scanner and connect it to the workstation Ettan DIGE Imager User Manual 11 0036 59 Edition AC 11 2 Installing the Scanner 2 2 Set Up the Scanner 2 2 1 Installation Requirements and Recommendations Before you install the scanner make sure that your facility supports the following scanner requirements Electrical Requirements Voltage 100 240 VAC 10 Circuit Rating 100 V 5 6 A 240 V 2 1 A Transient over voltages Must be in accordance with Installation Category II in IEC 664 Operating Environment Indoor use Requirements Temperature 59 90 F 15 32 C daily variation of no more than 3 F 1 8 C Humidity Maximum
43. gel storage solution and the upper plate applied In either case the outer plates need rinsing with de ionized water and then wiping dry with lint free tissues before being placed in the cassette Follow the instructions for assembled gels Naked gels or gels other than Ettan DALT and SE600 For naked gels and gels other than Ettan DALT or SE 600 the cassette with the permanently mounted glass plate Mounted Glass Cassette should be used 1 Clean the glass plate with distilled water and dry it using a lint free cloth Since fluorescent material has come into direct contact with the glass plate we recommend using a lint free tissue moistened with 10 hydrogen peroxide to remove this material followed by cleaning with distilled water 2 Place the gel s directly on the plate First squirt a small amount of distilled water onto the plate taking care to exclude air bubbles as the gel is positioned Note Up to six small gels can be placed on the gel plate 3 Note the positions of the gel boundaries on the plate See Fig 3 5 You will use this information to determine the area s to be scanned see 3 3 Set Up a scan Ettan DIGE Imager User Manual 11 0036 59 Edition AC 35 3 Getting Started 3 2 Prepare the Cassette for a scan 36 Fig 3 5 Marking gel position on a standard cassette 4 Putthe lid on the cassette see Fig 3 4 and close the cassette by turning the locking cams up Membranes When scanning membranes the cassette
44. gt n 12345 6 7 8 9 101112131415 161718192021 2223 24 25 26 27 28 29 30 31 3233 34 35 36 Uneditable Gel Format peecrendanapanahowstrandonaton e es FS Fea FC A ES fed S Pe ist fee al eet ead een bec H H ry i ict a wore 12345 6 7 8 9 101112131415 161718192021 2223 24 25 26 27 28 29 30 31 3233 34 35 36 Hoefer miniVE Ettan DALT 3 3 5 12345 67 8 9 101112131415 161718192021 2223 24 25 26 27 28 29 30 31 32 33 34 35 36 Hoefer SE600 Fig 4 1 Standard Gel Formats Create Custom Gel Formats 4 2 2 This is especially naked gels a You can create custom gel formats to scan irregular areas useful when you are scanning To create a custom gel format based on the Make sure that the Open the cassette and note the position of the gel areas numbers and letters on the side of the cassette 1 Then load rectangles formed by the coordinates cover the entire sample the cassette into the scanner 5 Ettan DIGE Imager User Manual 11 0036 59 Edition AC 4 Customizing Templates and Gel Formats 4 2 Standard and Custom Gel Formats Fig 4 2 The gel area 2 Inthe Gel Format list on the Ettan DIGE Imager window Setup area choose User Select 3 Click and drag the mouse across the grid area to create new scan areas that match the areas on the cassette 58 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Customizing Templates and Gel Formats 4
45. hone and Modem Options 5 j Hii gt Create a connection to the network at your workplace Troubleshooters sm B Set up a wireless network for a home or small office 2 Networking gt Change Windows Firewall settings A Internet Explorer F Network Diagnostics or pick a Control Panel icon gt k m Internet Options Network Connections Windows Firewall Wireless Network Setup Wizard 5 Onthe right side of the Network Connections window select Local Area Connection 2 Then select Change settings of this connection under Network Tasks Network Connections File Edit View Favorites Tools Advanced Help G a pas ch Reems Network Tasks Create a new connection 5 n m change Windows Local Area Connection 2 Firewall settings e Disable this network device Repair this connection mij Rename this connection View status of this connection Other Places E Control Panel 20 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Installing the Scanner 2 6 Inthe Internet Protocol TCP IP Properties dialog box select Internet Protocol TCP IP Local Area Connection 2 Properties I General Authentication Advanced Connect using Eg Broadcom Net treme Gigabit Ethene This connection uses the following items El Client for Microsoft Networks im File and Printer Sharing for Microsoft Networks Eos Packet Scheduler Internet Protocol T CPYIP Install Uninstall
46. ide the range to the left or right click and drag on the center control point Image Scaling Channel C cv2 ceva O cys File Min 0 0 Max 531456 0 Display Min 6644 5 Max 44227453 Gamma Restore Default Scale Calculate Histogram Logarithmic Histogram Center Control Point 531556 0 Cancel Fig 5 4 Image Contrast center control point 5 Tochange the intensity scale distribution click anywhere in the histogram except on the control points and drag the mouse up or down 64 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Using the Report Viewer 5 E Image Scaling Channel CQ cv2 cys O cvs File Min 0 0 Max 53156 0 Display Min 6644 5 Max 44227 453 Gamma Restore Default Scale Calculate Histogram Logarithmic Hiztogram 203080 0 0 63 1756 0 acces coca Fig 5 5 Image Contrast scale distribution 6 To undo all of the contrast changes click Cancel 7 Tosave the contrast settings click OK 5 3 Display the Image Scale Bar You can display or hide a scale bar to show the scale of the image You can also set options to move the scale bar to show the scale of a point of interest in the image or to control how the scale bar is displayed The scale bar is not displayed by default To change the image scale bar 1 Choose View Scale Bar from the menu to open the Scale Bar dialog Scale Bar EA t Y Position gt Thickness 1 RA Calor ens
47. indow 28401414 84 Ettan DIGE Imager User Manual 11 0036 59 Edition AC For contact information for your local office please visit www gelifescience com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com imagination at work i Elanders i Uppsala 2008 Ettan and DeCyder are trademarks of GE Healthcare Ltd GE tagline and GE monogram are trademarks of General Electric Company All third party trademarks are the property of their respective owners 2004 2007 General Electric Company All rights reserved First published Jan 2004 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 11003659 AC 09 2007
48. l i Lt i REY Soe skesbockedsstockedodssteskodssdssl i OR fb RER Eb bb bb Eb od oi i hie pul E ewes ec lee est aa a i Ta Ena a a E RR 1 Al io Torr Gat eas a te T Jaroor T m T FEST T arar T BE Fig 3 14 Grid Area 3 Chemistry When scanning membranes the Chemistry should be Unassigned 4 Pixel Size Select the pixels size i e resolution to use in the Pixel size list 100 um should be used 5 Channels and dyes Select the number of channels to be scanned by clicking the Channel check box Between one and four channels can be programmed Selection of the Channel check boxes must be performed in sequential numerical order For each selected channel select a dye to be scanned in that channel When selecting a dye the excitation and emission filter to be used for that dye are presented automatically by the software The excitation and emission filter combinations have been selected to deliver optimum results with a minimum of crosstalk Filter Details Standard Exposure Em Fiter rim Ex Fiter rim Dye Channel 595125 cys w 540 25 M BS0 50 CYS 635 30 M 2 0 BAU l co if 5 4 User Mame GE Healthcare Experiment Comments Fig 3 15 Filter settings Ettan DIGE Imager User Manual 11 0036 59 Edition AC 44 Getting Started 3 6 Exposure Select the exposure to be used for each channel in the Exposure list Generally low exposures are used f
49. l buffers and fixatives such as acetic acid ethanol and PBS 3 2 1 Types of Cassettes Gels must be scanned in a cassette Different types of cassettes are available for different purposes The Ettan DIGE Imager can be used with the following cassettes e Ettan DALT Cassette Ettan DIGE Imager 276x212 art no 11002704 e SE600 Cassette Ettan DIGE Imager 180x160 art no 11002732 e Mounted Glass Cassette Ettan DIGE Imager LF glass art no 11002733 Ettan DALT Cassette SE600 Cassette Mounted Glass Cassette Fig 3 2 Types of Cassettes Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 3 2 2 Loading a cassette Assembled Ettan DALT or SE600 gels For Ettan DIGE Imager applications the recommended glass plates have low fluorescence characteristics This allows the gels to be scanned while still assembled within the plates There are a number of advantages e Manipulation is easier and there is less risk of damaging the gel e Scans may be performed prior to running the bromophenol blue dye front off the gel so images of low molecular weight proteins that may otherwise be lost can be obtained Gels may then be replaced in the electrophoresis unit to complete the run Similarly runs can be extended and multiple images captured to obtain greater separation of higher molecular weight proteins Note Wear powder free gloves The powder used in laboratory gloves can fluoresce and may also scatter light affec
50. ns WARNING The computer should be installed and used according to the instructions provided by the manufacturer of the computer WARNING The mains power switch or other disconnect device must always be easy to access CE certification This product complies with the European directives listed below by fulfilling corresponding standards A copy of the Declaration of Conformity is available on request e 73 23 EEC Low Voltage Directive e 89 336 EEC EMC Directive The CE logo and corresponding declaration of conformity is valid for the instrument when it is e used as a stand alone unit or e connected to other CE marked GE Healthcare instruments or e connected to other products recommended or described in this manual and e used in the same state as it was delivered from GEHealthcare except for alterations described in this manual Note The Declaration of conformity is valid only for systems that are marked with the CE logo CE Contents 1 Important User Information LL Wamngsand Cautions sisser rener neers 5 sl OUNO seer ev nS SOT RU a 5 121 Ultraviolet LIGHUGNG Hedi vusisnsssansrsrrpetiavn sked nde srt ttan ber 5 122 COTO ONG Warning LODE S scscvcutsacscstesseinesstastinccorioniesnaivveritanivnntion f 13 CET O a ET 9 2 Installing the Scanner al POUT US Scannere siennas NR 11 22 SO TS SONG nesenas anin a 11 AN Installation Requirements and Recommendations 12 Cae MINI
51. ole area with 100 um resolution If the target is not completely flat put a glass plate on top Recommended settings are Cy2 channel 0 12 exposure level The result should be an image similar to the one in Fig 8 1 Test result and the resulting image can be evaluated to check for the following issues Stitching accuracy All horizontal and vertical lines should appear stepless in the image Camera focus With 100 um resolution one line should not cover more than 2 pixels Pixel size accuracy The distance printed on the test target should match the distances measured with an image evaluation program The test target should be stored in a dark place Fig 8 1 Test result Note Other problems should be handled by GE Healthcare service personnel Ettan DIGE Imager User Manual 11 0036 59 Edition AC 81 8 Troubleshooting 8 1 Customer verification test target 82 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Appendix A A 1 Filter Details 100 80 T S 60 S 40 i we 20 0 200 300 400 500 600 700 800 Wavelenath nm Dye Excitation Filter Emission Filter a a rm o Cy3 540 25nm D 595 25nm M Gye Sm oo 00 EZ Deep Purple 1 540 25 nm 595 25 nm M Deep Purple 2 390 20nm ii 595 25nm E SYPRO Ruby 1 480 30nm FPB 595 25nm E SYPRO Ruby 2 390 20nm i 595 25nm E Ettan DIGE Imager User Manual 11 0036 59 Edition AC 83 A A 2 Spare Parts A 2 Spare Parts Lamp Assembly 28401403 Protective glass w
52. on Scanning E_coli 1 The Navigation Bar Instrument status I Si l 2 The STOP button Estimated Scan time min sec Estimated Disk Space Needed MB 59 7 Total Disk Space Available MB 12751 26 Scan Progress Area 1 of 1 Time Left 36 16 23 Q a fa bX Ww ia LJ 3 The Information Area CY 5 DeepPurplet Gel Format Ettan DALT DIGE File naming format is not selected Chemistry DIGE_min Matrix Type Gel Pixel Size 100 0 um User GE Healthcare Channel Dye Ex Filter nm Em Filter nm Exposure Standard cy2 480 30 530 40 0 800 CY3 540 25 595 25 0 200 cys 635 30 680 30 0 300 Channel CYS Last Panel minmaxmean 93 00 13884800175738 fT 4 The Status Bar 6 The Scan Summary Area Fig 3 19 The Scan View 1 The Navigation Bar indicates Scan View 2 The STOP button interrupts a scan 3 The Information Area displays the estimated scan time and the progress of the scan 4 The Status Bar displays the channel intensity ranges and saturation values for each panel after it is scanned 5 The View Area displays scan data for each channel as it is scanned Ettan DIGE Imager User Manual 11 0036 59 Edition AC 49 3 Getting Started 3 4 Scan a sample 50 6 The Scan Summary Area at the bottom right corner shows the scan settings while the samples are being scanned monitor the data quality of each channel in the View Area and Status bar The data is scanned in horizontal
53. or membranes However a quick test scan on a small area should be performed to identify an optimal exposure See Finding the optimal settings in section 3 4 1 7 Comments You can optionally add comments to your data To add comments to the data 1 1Atthe bottom of the Setup Area click Experiment Comments to open the Experiment Comments dialog box 2 2 Enter the Membrane ID the Individual Source and the Sample ID 3 3 Enter Comments for the scan To use the same comments for all the gels in this scan select Use this Comment for all Gels This option only applies to the Comments field All other fields are always specific to each gel area 4 4 Click Close To start the scan and for more information see Start a scan and following chapters 3 4 Scan a sample 3 4 1 Finding the optimal settings The exposure can be set from 0 02 to 4 The exposure chosen depends on the type and quantity of fluorophore present A test scan scanning only a small area should be performed to identify a suitable exposure This allows a rapid scan that will help finding the optimal settings The area should be placed somewhere on the gel were the most intense spots are supposed to be CAUTION The maximum pixel value should not exceed 65 000 counts If some of the pixel values are equal to or greater than 65 000 counts part of your image is at or near saturation This will prevent correct quantitative analysis For the E coli model system used a
54. p assembly to cool before handling the assembly e Donot touch the bulb Use lens tissue or a lint free cloth to handle the bulb Clean fingerprints from the bulb using an organic solvent such as ethanol and a lint free cloth Note The rear panel is equipped with a safety interlock If you open the rear panel while the lamp is turned on the safety interlock turns off the lamp WARNING Under certain conditions the bulb can explode If this occurs the fumes can be toxic Evacuate the room immediately and remain out of the room for at least 30 minutes 7 4 2 Overall steps for replacing the lamp assembly A Turn off the scanner and allow the lamp assembly to cool B Replace the assembly C Reset the bulb age counter in the scanner software D Perform a flat field calibration WARNING The lamp assembly can get extremely hot Make sure that the lamp is turned off and has cooled for at least fifteen minutes before replacing the lamp assembly To change a lamp assembly 1 Turn off the scanner as follows e Make sure that there is not a cassette in the scanner e Turn off the scanner e f you just turned off the lamp wait fifteen minutes for it to cool Ettan DIGE Imager User Manual 11 0036 59 Edition AC Maintaining the System 7 2 Replace the lamp assembly e Remove the rear panel of the scanner by loosening the two screws at the bottom of the panel Fig 7 2 Removing the rear panel screws e Gently pull
55. rayscale values are mapped to intensity values that are distributed unevenly throughout the range To change the intensity scale 1 Inthe Report Viewer window click W to open the Image Contrast window This window shows the image intensity scale The histogram Is a frequency plot that shows the distribution of pixel intensities in the image file The Y axis shows the number of pixels for a given intensity Image Scaling Channel O eva cya O cys File Min 0 0 Max 531560 Display Minoo OO Max 47757 3344 amma EJ Calculate Histogram Logarithmic Histograrn 203959 Fig 5 2 The Image Contrast window 2 Select a Channel option to specify which channel to scale in our example we selected Cy3 3 To change the minimum or maximum scale value click and drag on the left or right white control point Ettan DIGE Imager User Manual 11 0036 59 Edition AC 63 5 Using the Report Viewer 5 2 Set the Image Contrast Image Scaling Channel C cv2 cv3a O cys File Min 0 0 Max 53156 0 Display Min 6644 5 Max 44227 453 Gamma Restore Default Scale Calculate Histogram Logarithmic Histogram Control Points 531556 0 Cancel Fig 5 3 Image Contrast left and right control points As you move the control points the displayed image changes interactively Data values higher than the maximum value are assigned the lightest shade Values lower than the minimum value are assigned the darkest shade 4 Tosl
56. rce unspecified User comment unspecified Wiser name GE Healthcare Prep Date pras File CData ErikalScreen shotsi2 380_EDI din27380_ED ds Fig 5 9 Region of Interest ROI 3 Inthe Edit menu choose Crop The image is cropped to include only the data that is within the ROI 5 5 3 Restore the Image You can restore the image to its original state To restore the Image e In the Edit menu choose Restore The image is restored to its original position and size 5 5 4 Save the Modified Data You can save the modified data to a new data set Ettan DIGE Imager User Manual 11 0036 59 Edition AC 69 5 Using the Report Viewer 5 5 Rotate or Crop the Image To save the data 1 Onthe File menu choose Save As to save the dataset File Name Gataset x Select Folder B Desktop GE Healthcare H Sci H i My Documents BH i My Network Places Dataset Name DIGE gel 2563 n Fig 5 10 File Name Dataset dialog box 2 Inthe File Name Dataset dialog box browse to the directory in which to save the dataset Then enter the Dataset Name and click Save 5 5 5 Improve Report Viewer performance You can improve the performance of Report Viewer tools by allocating a second memory buffer in the Preferences dialog box To improve performance of Report Viewer tools 1 From the Ettan DIGE Imager window Edit menu choose Preferences 2 On the Preferences Dialog box select Allocate second buffer for quick
57. rom one place to another Static charges are easily generated and can be stored on people and various materials including plain plastics People often carry charges of 1000 to 5000 V but do not feel the sensation of a discharge under 3000 V Plastics can hold charges of several hundred to 25 000 V A few hundred volts can damage most semiconductor devices in a fraction of a microsecond CAUTION When you turn off the scanner keep the power cord plugged in so that the system is electrically grounded The system contains microelectronic devices that can be damaged by electrostatic discharge 7 2 Clean the System Wipe the surfaces of the scanner as needed with a lint free cloth slightly moistened with water or 10 ethanol in water 7 3 Replace the Protective Window The Protective Window is a sealed glass window that protects the optics from spills and exposure to cleaning fluids It fits over the camera lens If the window is scratched or damaged in any other way order a new window and use the following instructions to replace it Ettan DIGE Imager User Manual 11 0036 59 Edition AC 7s 7 Maintaining the System 7 3 Replace the Protective Window Fig 7 1 The Protective Window To replace the Protective Window 1 Turn off the scanner 2 Ifa cassette is on the stage remove it WN Slide the stage to the right to expose the Protective window Remove the two screws that hold the window to the stage platform 5 Inse
58. rt a screwdriver under the indented slot and lift the edge of the window up to remove it 74 Ettan DIGE Imager User Manual 11 0036 59 Edition AC 6 Place the new window on the o ring in the window holder 7 Tighten the two screws that hold the window in place until they are snug 8 Turn onthe scanner The stage homes itself to its original position ay hh D OM PAPA P O J AMA A Cc gt Ga FR OMIMNMFO rre TIDO AS Tri l bn INS NM JULLE UU J CL d J ug 8 mOrA EE LJ LJ The lamp assembly provides light to the optical system The assembly consists of a metal halide bulb and lamp housing After 1000 hours of use the bulb in the lamp assembly can no longer supply the intensity and spectral range of light required by the scanner s optics In order to ensure that the bulb is properly aligned in the optical system replace the entire assembly as a unit Ettan DIGE Imager User Manual 11 0036 59 Edition AC 15 7 Maintaining the System 7 4 Replace the Lamp Assembly 76 7 4 1 Safety There are three risks associated with handling the lamp assembly exposure to heat exposure to ultraviolet light and exposure to mercury vapor Oil from fingerprints on the bulb can heat up and cause the bulb to explode when it is turned on releasing dangerous vapor Observe the following safety precautions when handling the bulb assembly e Do not open the lamp access door while the lamp is turned on e Wait for the lam
59. t GE Healthcare 50 ug protein labeled with 400 pmol dye run on 24 cm pH4 7 NL Immobiline DryStrips strips that are then used with the second dimension run on Ettan DALT gels the following exposure levels can be used as guidelines Ettan DIGE Imager User Manual 11 0036 59 Edition AC 45 3 Getting Started 3 4 Scan a sample Fig 3 16 Choosing a small test scan area Fluorophore Exposure levels Cy2 0 8 Cy3 0 3 Cys OS Fig 3 17 Scan finished 46 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Getting Started 3 A target signal of 30 000 to 55 000 is usually suitable Similarly if only one or two spots show saturation then only minor downward adjustments to the exposure setting are normally required It is not necessary to optimize scan settings for every gel To avoid problems with saturated images it is recommended that users confirm that suitable image signals have been obtained before discarding gels Once the exposure has been selected for one gel in an experiment all similar gels within the same experiment should be scanned using the same exposure After you have entered the settings for the scan use the following instructions to scan the sample 3 4 2 Start a scan 1 Make sure that the Instrument status in the Information area displays Ready and that the READY light on the scanner is on 2 Ifthe Instrument status is Lamp Off click 9 on the toolbar or go to the menu under Instrument Toggle Lamp
60. ther Control Panel ee Options aa Add or Remove PAE Date Time Language and Regional gE Options z J Sounds Speech and Audio Devices Accessibility Options i r Performance and Maintenance y Security Center Note Ifthe Control Panel view is different from the view shown above click Switch to Category View amp Control Panel File Edt View Favorites Tools Help 2 dt senene Vi Control Panel a E nte intl Braio Moas maaa aema G Administrative Tools Configure administr Automatic Updates Set up Windows to See Also pa Date and Time Set the date time wa Windows Update Display Genes Rie yrs Ps Help and Support in a amen ak change vee my Game Controllers Add remove and c es Control the graphic Configure your Inte Customize your key Microsoft Outlook P Customize your mo a Connects to other c Configure your tele View the po able mM Ettan DIGE Imager User Manual 11 0036 59 Edition AC 19 2 Installing the Scanner 2 5 Setup the Workstation 4 In the Network and Internet window select Network Connections E Network and Internet Connections mB File Edit View Favorites Tools Help Ons QB Asah roes Address GQ Network and Internet Connections wat Network and Internet Connections See Also My Network Places fp Printers and other Pick a task Hardware a aaah gt Set h Internet ti Set up or change your Internet connection b P
61. ting image quality e Use the Ettan DALT Cassette for an assembled Ettan DALT gel e Use the SE600 Cassette for an assembled SE600 gel To load a cassette 1 Clean the gel glass plates with distilled water using a lint free cloth It is important that the glass plates are clean dry and free from lint If necessary clean the inside lid cover of the cassette with distilled water 2 Insert the dried glass plates into the cassette with the shorter plate facing down See figure 3 3 Inserting plates in a DALT Cassette The shorter plate should rest in the seal at the bottom of the cassette The large plate should hang over the area at the rear of the cassette Note The positioning of the strip on the gel is of importance in order to achieve the correct orientation of your image After inserting the gel into the cassette and looking from above if the acidic side of the strip is pointing to the right the image needs to be flipped to achieve the correct orientation This can easily be done using the Report viewer see 5 5 Rotate or Crop the Image Note For experimental guidelines refer to the Ettan DIGE System User Manual 18 1173 17 Ettan DIGE Imager User Manual 11 0036 59 Edition AC 33 3 Getting Started 3 2 Prepare the Cassette for a scan Fig 3 3 Inserting plates in a DALT Cassette 3 Put the lid on the cassette and close the cassette by turning the locking cams up as illustrated in Fig 3 4 Fig 3 4 Closing the cassette
62. ttan DIGE Imager User Manual 11 0036 59 Edition AC 5 4 59 Sd 5 22 iso 5 5 4 To Measure DISTANCES oouceeccccecssscscssssesesssesesesesecesesesesesesesesesesesescstseecansceeee 66 Rotate or Crop the WO 0 e xacecc cane nnneeentnee 68 Rotate TOTTI OS cscsctccicehstsecsnierstsresistncspshssedsienasdtadecsniarntvnsisrieneauiisienats 68 Crop Ne MOOC seis pennant rst NN avn R 68 Restore the MOOG nuuanu NRA 69 SOV OIG DOO 6 raghishctencrcieaasssntsdtitstarntehdceiesdicdeaeander 69 Improve Report Viewer performance 70 6 File formats 6 1 6 2 6 3 DIGE File Naming FOR MIOL ss csisssscsstecssstsstossrosconisnsseledeswsstasnstiocenseussiesacs 71 Standard File NAMING FOrMOt eecccccsessssesssesssesssessesssesssecsseeeseeeses l GE TIS TOMT ICT senres ssh 71 7 Maintaining the System fak 7 2 ee 74 7 4 1 7 4 2 Electrostatic Discharge ESD um 3 Clean the SUSTEM ses scares teas cecaczoustceeclac tecesees tendons oauaresanceneeee 73 Replace the Protective WINCIOW us Ts Replace the LAMP Assembly ssssssssssessesssssrasrrrrrrrrrnnnnnrn nere nnn nn rna ann fas DOO L PSIE EA ree arene cara eae 76 Overall steps for replacing the lamp assembly ssssss 76 8 Troubleshooting 8 1 Customer verification test target wn 81 Appendix A Al CST DOUS gismu ereen ERE 83 Ac opare PONS gee ee ee ee ee RCE EE 84 Ettan DIGE Imager User Manual 11 0036 59 Edition AC Preface Preface Ettan DIGE Imager Ettan

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