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(RAS) - 100 - McLane Research Laboratories, Inc.

1. Figure 7 23 Pre amp Post Sample Flush Exceeds Available Acid Volume cLANE 7 13 RESEARCH LABORATORIES INC Checking for Event Overlap After a schedule is accepted the RAS 100 firmware checks for potential problems such as expired or overlapping events If an overlap occurs during deployment the start of the next event is delayed until the current event is completed an overlap condition does not terminate the deployment Event overlap is calculated after the sampling parameters have been entered and is based on the acid exposure delay and the duration of the combined flushing and sample volumes at their minimum flow rates The firmware compares the time limit of each event to the scheduled start time of each subsequent event The warning message indicates that an overlap could occur not that it will Entering a schedule with intentionally short intervals can be a technique to secure rapid sequential samples The overlap message is only a reminder and can be ignored if tight scheduling is intended The following event s overlap previous event s 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 The listed events may begin later than their scheduled start Any events that are actually delayed will start as soon as earlier events are completed Press any key to continue Press any key to continue Figure 7 24 Overlap Reminder 7 14 cLANE RESEARCH
2. Tue Aug 30 ET 2005 Port lt l gt Set Time lt 5 gt Create Schedule lt 2 gt Diagnostics lt 6 gt Deploy System lt 3 gt Manual Operation lt 7 gt Offload Data lt 4 gt Sleep lt 8 gt Contacting McLane Selection c Password configuration lt l gt Internal temperature probe Enabled lt 2 gt External temperature probe Disabled lt X gt Exit Selection 2 Enable external temperature probe Yes No N Y Disabling internal temperature probe Configuration lt l gt Internal temperature probe Disabled lt 2 gt External temperature probe Enabled lt X gt Exit Selection x Figure B 3 System Configuration Menu Temperature Configuration Appendix B 2 cLANE RESEARCH LABORATORIES INC 2 Select lt 2 gt to enable the External 12 4K Temperature Probe 3 Type Y at the Enable Temperature Probe prompt and type X to exit and save the change The Temperature Probe configuration is stored in EEPROM and used the next time the firmware is started alc LANE Appendix B 3 Appendix B 4 KalMcLANE
3. 1 Place an Acid Bag in the sample chamber Use a syringe to inject acid into the bag 2 Attach the sample bag to the sample chamber cap and close the sample chamber 3 Remove the pressure compensation tube from this cap 4 From the Manual Operation menu select lt 2 gt Find port 49 5 Select lt 6 gt Run pump reverse until the sample chamber for Port 49 fills with water and water drips out of the bleed hole 6 Install the pressure compensation tube into the bleed hole 5 10 cLANE RESEARCH LABORATORIES INC Step 5 Installing the Water Flush Filter The water flush filter is placed inline to Home Port Port 0 to hold a 25mm disc filter with a pore size from 3 to 20 microns The filter protects the valve from damage by large particles To install the water flush filter complete the following steps 8 9 Figure 5 11 Water Flush Filter Holder Place the pump exhaust tube in a bucket of water if not already in water Remove the intake plug from the valve intake and place a syringe filled with water in the valve intake Disassemble the water flush filter by unscrewing the filter cap From the Manual Operation menu select lt 1 gt Find port home From the Manual Operation menu select lt 6 gt Run pump reverse Run the pump until water flows out of the filter holder Push the syringe injecting water into the tubing and through the filter holder frit Place the filter in the filter holder on top
4. 5 8 cLANE RESEARCH LABORATORIES INC 2 Using a hose connected to tap water fill the sample chamber to the top with water 3 Place the top back on the sample chamber and re secure If using Luer locking valve bags open the bag valve before re securing the sample chamber cap 4 Repeat steps 3 for each sample chamber 5 Connect one end of the exhaust tube provided in Toolkit to the pump exhaust 6 Place the other end of the exhaust tube in a sink or large bucket filled with water and keep the tube submerged throughout the procedure to prevent air bubbles from entering the tubing 7 Remove the plug from the side of the sample chamber 8 From the Manual Operation menu select lt 2 gt Find port 1 9 From the Manual Operation menu select lt 6 gt Run pump reverse 10 Run the pump until water drips from the bleed hole and re insert the bleed hole plug 11 From the Manual Operation menu select lt 3 gt Next port advance 12 Repeat steps 7 12 for Ports 2 through 48 EaVicLANE 5 9 Step 4 Adding Acid Flush to Sample Chamber Number 49 Adding acid to Port 49 provides a bio fouling flush that further cleans the intake tube When this step is completed the intake plug should be on the valve intake and the exhaust tubing should still be in a sink or bucket of water as was setup in Step 3 Filling Sample Chambers with Water and Reverse Pumping To add acid complete the following steps
5. Modify an event Yes No N 01 16 2007 17 48 00 01 16 2007 17 51 00 Figure 7 21 Event Verification To change an event type Y at the Modify an event prompt Select the event and enter a new date and time Type N or press ENTER to proceed with the deployment Programming Sampling Parameters When deployment initialization and scheduling is completed a menu is displayed for entering the sampling parameters After entering the parameters described below select D to continue with the deployment The parameters are divided by general function into five groups Header Acid Water Sample and Timing Header A B C Acid D Pre sample acid flush Enabled E Flushing volume 5 ml F Flushing time limit 1 min G Exposure time delay 1 min water H Flushing volume 10 ml I Flushing time limit 1 min Sample J Sample volume z 25 ml K Sample time limit 2 min Acid L Post sample acid flush Enabled M Flushing volume 5 ml N Flushing time limit 1 min Timing P Pump data period 1 min v Verify and proceed Selection Figure 7 22 Sampling Parameters A Quick Reference overview of each sampling parameter is provided next More detailed descriptions of the deployment parameters follow the Quick Reference 7 10 RAS 100 Sampling Parameters Quick Reference Header Three lines of file header text up to 65 characters per line
6. Defaults to maximum value and allows a smaller value Post sample Acid Flush Status of post sample flush Enabled or Disabled Post sample Flushing Volume Amount of acid flushed through the intake determined by the number of samples pre acid flush volume and acid bag volume 480 ml Example 48 samples no pre acid flush equals maximum allowed pre sample flush volume of 10 ml Post Sample Flushing Time Limit Maximum time for the acid flush prior to sampling Allowed value depends on flushing volume and the maximum and minimum flow rates 75 ml min and 20 ml min Defaults to maximum value and allows a smaller value Pump Data Period Sampling period stored during each event pump flow rates volumes EaVicLANE 7 11 Detailed Descriptions of Sampling Parameters Option lt A gt lt B gt and lt C gt Header Enter a header or a personalized title for each deployment Each header line may contain up to 65 characters Option lt D gt Pre sample Acid Flush This option enables or disables the pre sample acid flush Option lt E gt Acid Flushing Volume This option specifies the volume of acid or other bio fouling fluid to flush through the valve intake before taking a sample Acid flushing loosens any bio fouling growth along the intake path so that it can be removed by the water flush The maximum volume is the capacity of the nom
7. stop the Crosscut Capture to file The Offload option can be executed again if necessary the data file remains in memory until a new deployment schedule is created or the batteries are disconnected cLANE RESEARCH LABORATORIES INC 8 3 EaVicLANE Appendix A Operating Crosscut and Crosscut for Windows File logging cab be used during all interactions with the RAS 100 to create a log of operations deployment settings and recovery procedures There are two standard file capture programs that McLane recommends Both programs are freely distributed by Onset Computer www onsetcomp com for TT8 communication Crosscut is a DOS based program that runs on a PC and Crosscut for Win is Windows based McLane recommends using file capture for all deployments To download compressed archives of Crosscut and Crosscut for Win software go to www mclanelabs com downloads crosscut zip www mclanelabs com downloads crosscut win zip Using Crosscut Crosscut is a DOS based program that runs on a PC Crosscut will run without modification or difficulty under DOS Windows up to Windows 98 1st edition and Windows NT up to Version 4 0 Service Pack 5 Later releases of these operating systems commonly require a change to the PC registry to disable power management of the serial port Modifications may also be required to enable the mouse Detailed instructions for the registry change can be found below and at www mclanelabs com lapt
8. Enter each event time ai vac craic tipccss oddest eevee cectonrspeeaclcsamaess 7 6 Option lt 2 gt Enter start date amp interval sc s45 2csscisspadancdecsdavscdastaevisaanaciaceplandacevaetiast 7 6 Option lt 3 gt Enter start date amp end date c ho saiie cstv civeietccusnaew acess 7 6 SO Deploy Syste M ere e aoa ce cca A Sac stots alc eas Bas Bases e A AG 7 7 Deployment Initialization o2 245 03 cak oases nee aca twaaes 7 7 Entering or Changing a Pumping Schedule cece ecccecsseceteceeeeeeeeeeeeeneeeteenees 7 9 Programming Sampling Parameters 00 ccccceecceesceenceeeseceeeceeeeeeseeeaeeeeeeneeneaes 7 10 RAS 100 Sampling Parameters x iiss aacnoeiunenusien ee aaa aa eats 7 11 Detailed Descriptions of Sampling Parameters c ccccccesceeeceeseeeseeeeeeeeeeeeeeeenaees 7 12 Option lt A gt lt B gt and lt C gt Header a icccr set taceSveartdeasuhcuniadas thet suntaeddavatdansniaandanetes 7 12 Option lt D gt Pre sample Acid Flush ccc cccccessecsteceseceeeeeesceceseecaeceeeseeeeeaeees 7 12 Option lt E gt Acid Flushing Volume sisiss chcseisacstauedesacaeiecepdanGnchsadsaitseeiecep ian Gativande 7 12 Option lt F gt Acid Flushing Time Limitasniesicinciducuateuseniininctveisact 7 12 Option lt G gt Acid Exposure Time Delay ssssesssssessseesreseesseeseesresseeserssressessrssees 7 12 Option lt H gt Water Flushing Volume ssssssssenseesesseeseeseesseesresessseesseserssressessrssees 7 12 Option lt I gt W
9. INC 4 4 EaVicLANE Chapter 5 Operations Prior to deployment the RAS 100 fluid lines and sample chambers must be cleaned and the sampler must be primed to remove air Information to determine instrument current consumption to confirm sufficient battery life for the deployment is also provided in this chapter Cleaning the Fluid Lines and Sample Chambers Cleaning the RAS 100 fluid lines removes contaminants and air pockets To flush the fluid lines with cleaning solution complete the following steps 1 Remove all bags from the acrylic sample blocks and replace the caps 2 Place the intake and exhaust hoses into a container of prepared cleaning solution 3 From the Main Menu select lt 3 gt Manual Operation Manual Operation Tue Jan 16 15 20 37 2007 Port 00 lt l gt Find port home lt 2 gt Find port J lt 3 gt Next port advance lt 4 gt Next port retreat lt 5 gt Run pump forward 100 ml lt 6 gt Run pump reverse 100 ml lt 7 gt Run pump programmable lt M gt Main Menu Selection 3 Valve moving to port 01 port 00 01 Port 01 located performing alignment done Alignment confirmed Figure 5 1 Manual Operation 4 From the Manual Operation menu select lt 1 gt Find Port home to move the valve to the Home Port 0 A message displays once the port is located 5 From the Manual Operation menu select lt 5 gt Run Pump forward to flush cleaning solution through
10. Inthe prompt box type regedit and click OK to start the registry editor window 3 The editor will display a Windows directory tree in the left half of the window and a folder contents display in the right half of the window Click through the directory tree following this path HKEY LOCAL MACHINE SYSTEM CurrentControlSet Services VxD VCOMM 4 Click on the VCOMM folder and its contents will be displayed in the right half of the window 5 Double click ENABLE POWER MANAGEMENT in the right half of the window and change its value from 0 00 00 00 to 00 00 00 00 using the editing tool that pops up 6 After the value has been changed go back through the directory tree and exit the registry editor 7 Exit all programs restart the PC and try Crosscut again Capturing Data Files Using Crosscut Once the steps for first time Crosscut use are complete use the steps provided next to capture data files 1 To start Crosscut type crosscut at a DOS prompt or click on the Crosscut icon the Crosscut directory must be in the directory path for this step a ae KAMSLANE 2 To capture a data file after a deployment select CommPort from the menu bar and Capture to File from the submenu 3 Select a directory and a name for the file 4 The Open file window will appear in the Crosscut window ALT Z brings up the Open file window from the keyboard 5 Type the path and name of the f
11. all connecting fluid lines at that port 6 Run the pump until cleaning solution has been flushed through the line EaVicLANE 5 1 7 Press CTRL C to stop the pump or wait for the program to finish pumping the specified volume 8 Select lt 3 gt Next Port advance to move the valve to the next port 9 Repeat steps 5 8 for each port 10 After all lines have been flushed with solution place the intake hose into a large reservoir of distilled water and place the exhaust hose into an empty waste water container drain 11 Select lt 5 gt Run Pump forward and rinse each line Select lt 5 gt to run the pump forward reverse pumping will draw waste water through the lines 12 After each line is rinsed stop the pump by pressing CTRL C Select lt 3 gt Next port advance to advance the valve 13 Repeat steps 11 12 for each sample chamber An optional flush with distilled water using steps 2 12 above can also be performed to further rinse each line 14 After the lines have been cleaned and rinsed remove each sample cap and clean the threads and o rings Inspect o rings visually and feel them for wear Priming the RAS 100 The RAS 100 lines and sample chambers must be clear of trapped air when deployed Sample caps can be installed with or without filter holders The priming steps detailed in this section provide an example of priming with filter holders installed Information about priming a RAS 100 w
12. and display to the screen the data is sent to the specified capture file An example data offload file is shown next Software version RAS100_5 Compiled Jan 5 5007 09 56 29 Internal Temp Electronics S N ML11111 01 Probe _____ty temperature probe Internal Data recording start time 01 16 2007 15 22 28 Data recording stop time 01 16 2007 18 17 26 HEADER SAMPLE PARAMETERS Pre sample gt pre sample acid acid flush Acid flush volume m1 5 Acid flush time limit minutes 1 Acid exposure delay minutes 1 water Flush water flush volume m1 10 water flush time limit minutes 1 Sample Sample volume m1 25 Post sample Sample time limit minutes 2 acid flush Post sample acid Acid flush volume m1 5 Acid flush time limit minutes 1 SCHEDULE Event 1 of 48 01 16 2007 15 30 00 Event 2 of 48 01 16 2007 15 33 00 Event 47 of 48 01 16 2007 17 48 00 Event 48 of 48 01 16 2007 17 51 00 DEPLOYMENT DATA 1 01 16 2007 15 30 00 31 5 vbat 20 6 C PORT 00 Pre sample acid flush 5 ml 5 sec LB 31 5 V Volume reached Flush port 49 Intake flush 10 ml 9 sec LB 31 5 V Volume reached Flush port 00 Sample 25 ml 21 sec LB 31 5 V Volume reached Sample port 01 01 16 2007 15 32 01 31 5 Vbat 20 9 C PORT 01 Post sample acid flush 5 ml 4 sec LB 31 5 v Volume reached Flush port 49 Figure 8 3 Data Offload File screen I of 2 IVIC LAN E 47 48 2 01 16
13. auxiliary battery is in place removing the main battery erases all the deployment data stored in memory Backup Battery The backup battery is a 9 volt alkaline battery The backup battery will sustain the controller memory for approximately 3 months in the event of a main battery failure Making contact with the battery terminals reversed can seriously damage the controller Install the backup battery such that the terminals of the battery correctly correspond to the terminals on the holder Storage The RAS 100 shipping crate is a reusable international freight container that is ISPM 15 compliant The crate is intended for both shipping and storing the RAS 100 and meets the requirements for international transport by ground ocean or air freight carriers To avoid instrument damage do not leave the RAS 100 sampler in direct sunlight for prolonged periods of time and avoid excessive vibration There are several procedures to prepare the RAS 100 for storage longer than a month e Offload all data from memory e Rinse all instrument components with fresh water e Remove both batteries If the main battery is still usable first cover the connector with insulation tape and then store the battery in a refrigerator e Reassemble the main battery holder and insert the electronics package back into the housing e Replace all bolts e Apply a small amount of anti seize to the threads cLANE 4 3 RESEARCH LABORATORIES
14. including 95 98 Me NT 2000 and XP Contact McLane or Onset Computer www onsetcomp com for additional information alc LANE Appendix A 3 First Time Crosscut for Win Use To use Crosscut for Win for the first time complete the following steps 1 Create a Crosscut for Win directory and copy into it the two Crosscut for Win files from the disk provided in the Toolkit 2 Click on the Crosscut for Win icon to run the program or establish a short cut from the desktop Do not connect the COM cable to the RAS 100 yet 3 Select CommPort from the menu bar at the top of the Crosscut for Win window and then Setup from the submenu 4 Select the COM port and set the port parameters to 9600 baud 8 data bits 1 stop bit and no parity 9600 8 N 1 5 Click OK These values are stored for use whenever Crosscut for Win is started in the future 6 Connect the COM cable from the PC to the RAS 100 serial port Capturing Data Files with Crosscut for Win 1 Start Crosscut for Win 2 To capture a data file after a deployment select Terminal from the menu bar and Capture Setup from the submenu 3 Enter a Capture file name in the box and select Overwrite or Append Overwrite replaces any information in an existing log file Append adds new information to an existing file select carefully 4 Click OK 5 Start File Capture Everything that appears in the Crosscut for Win
15. lt l gt Enter each event time lt 2 gt Enter start date amp interval lt 3 gt Enter start date amp end date lt M gt Main Menu Selection 2 Enter START date and time 01 30 2007 12 01 21 2 1 07 08 00 00 Enter interval Days CO to 365 0 Hours 0 to 23 0 Minutes 0 to 59 2 Schedule Verification Event 1 of 48 01 16 2007 15 18 11 Event 2 of 48 01 16 2007 15 20 11 Event 3 of 48 01 16 2007 15 22 11 Event 4 of 48 01 16 2007 15 24 11 Event 47 of 48 01 16 2007 16 50 11 Event 48 of 48 01 16 2007 16 52 11 Press any key to continue Figure 7 11 Schedule Menu There are several options for creating a sampling schedule of events Figure 7 11 shows option lt 2 gt The other options are also described below Option lt 1 gt Enter each event time This option allows individual sample event entry month day year hour minute and second The events do not have to be entered chronologically they are automatically sorted Option lt 2 gt Enter start date amp interval This option allows entry of a start date and a desired interval between the event start times The interval is entered in units of days hours and minutes Option lt 3 gt Enter start date amp end date This option schedules events at regular intervals between the entered start and end dates lt 6 gt Deploy System This option prepares the RAS 100 for a deployment Select Deploy System to redisplay M
16. offloaded with the data Pre sample acid flush Status of pre sample flush Enabled or Disabled Pre sample flushing volume Amount of acid flushed through the intake determined by the number of samples post acid flush volume and acid bag volume 480 ml Example 48 samples no post acid flush equals maximum allowed pre sample flush volume of 10 ml Pre sample flushing time limit Time limit for flush before the pump is turned off Exposure time delay Time delay during which acid remains in the valve intake removing growth Automatically set to zero when the acid flushing volume is set to zero Water Flushing Volume Amount of water flushed through the water port before sampling to remove bio growth loosened by the acid flush Water Flushing Time Limit Maximum time for the system flush prior to sampling Allowed value depends on the flushing volume and maximum and minimum flow rates 75 ml min and 20 ml min Defaults to maximum value and allows a smaller value Sample Volume Amount of water to be sampled before the pump is turned off Adjust flow rate with the control system to reduce differential pressure across the sampling apparatus Sample Time Limit The maximum sample time limit before pumping stops Allowed value depends on the sample volume and the maximum and minimum flow rates 75 ml min and 20 ml min
17. on the chassis plate between the controller housing end cap and the main battery cage aei RND o U Ge Ba stort Figure 3 1 RAS 100 Controller Stack Take standard electrostatic discharge ESD precautions when handling the electronics The top circuit board in the controller stack is a 3 Phase circuit which accepts a serial control command from the pump software and converts it to an analog control voltage to drive the pump motor Power to the motor is routed through the chip set from the main battery The chip set also monitors Hall effect sensors in the motor housing and sends a pulse train to the TT8 to monitor the actual pump speed The pump software completes a tight Proportional Integral PI control loop around the pump and tracks the programmed flow rate independent of battery voltage and other environmental forcing The pumped volume is monitored and limited to the capacity of a sample bag to protect the integrity of each sample cLANE 3 1 RESEARCH LABORATORIES INC The middle circuit board contains an AUX circuit and a Stepper circuit The AUX circuit generates regulated voltages from the main and auxiliary battery inputs and distributes voltages to the rest of the electronics under the control of the RAS 100 software The AUX circuit includes access to the RS 232 serial communications port of the TT8 and an on board thermistor used to monitor temperatures inside the controller pressure housing Optionally an ex
18. seinen iacs anaes 7 4 Fieure 7 9 Low Power Sleepeeccnen iinn iieii tas aps tease Ainge ena 7 5 Faeure 7 10 Cr ate Sched l smesso nranti as aar a KAERT EET KAE AEAT aA 7 6 KEIVic LANE LOF 1 Figure 7 11 Schedule Men as 2c atrsic totes caccencccem ante caeatas Gay tcuatuaee Ata de cancel gerctiavets 7 6 Figure 7 12 Confirming Home Port Alignment 00 ce ccceescesceeeeceeeceeeecceteeeseeseees 7 7 Figure 7 13 Unable to Locate Home Port scsi sic sicessesscensessdensesssvaccseasductetouncdesevuncenses 7 7 Figure 7 14 Data Set Exists in Memory scscsscsssessssnsenseesconscenssensssnsonsseaeessess 7 8 Figure 7 15 Real Time Clock x cc 6 teccags jasucgisntecvesssettcaxsedntn eadontania suteeepssbiaten dete vscadeces 7 8 Figure 7 16 Previous Deployment Data in Memory ccccecsceesseeeteceteeeeeeeseeeeeseees 7 8 Figure 7 17 Enter New Schedule i ccisc sccescassevsendectdeccsapunoustecsieteasivestatssovsevtaynenentedancas 7 8 Figure 7 18 Enter Number of Events to Program cecssceeseeeeceeeceseeneeeseeeeeeeeeeaees 7 9 Figure 7 19 Schedule Men c 3 c0 5 c00is sssfecS ansdnetisedectushseeveagetin deus uartiareeeniaeeicn 7 9 Figure 7 20 Pumping Start Date amp Time cee ccccecssceesceeeeceneceeeeeeseecaecneeeeeeenseees 7 9 IOUS 722 SE VCMY V ChIMC ARON 63 ean ee eA E 7 10 Figure 7 22 Sampling Paramicte rss sca cesses sy oceces sax vaacasosncehcvaas va pasansh gtecaacaveoeatavouaass 7 10 Figure 7 23 Pr
19. the valve and the sample chamber with water and then attach the sample bag to the sample chamber cap 5 Place the bag inside the sample chamber and attach the cap 6 Ifoptional filter holders are installed complete the following Remove the filter holder frit Fill the bottom reservoir of the filter holder with water Replace the filter holder frit Wet the filter and place on top of the frit Press down on the syringe in the valve intake to fill the tubing and filter holder top with water Push down on filter top to displace air bubbles and re secure the filter holder cap Pushing down on filter holder top Figure 5 9 Reassembling Filter Holder 7 From the Manual Operation menu select lt 3 gt Next port advance to move to the next port cLANE one RESEARCH LABORATORIES INC 8 Repeat steps 2 7 for each sample chamber 9 Remove the syringe from the valve intake and replace with the intake plug provided in the Toolkit Figure 5 10 Intake Plug Do not forget to remove the intake plug just prior to deploying the RAS 100 If the plug remains in place during the deployment the RAS 100 cannot pump properly Step 3 Filling Sample Chambers with Water and Reverse Pumping Filling the sample chambers with neutral water prevents damage and sample contamination from differential pressure while reverse pumping at each Port removes any trapped air 1 Open a sample chamber
20. 2007 15 33 00 31 5 vat 21 1 C PORT Pre sample acid flush m 5 sec LB Flush port 49 Intake flush 10 ml 9 sec LB 31 5 V Flush port 00 Sample 25 ml 21 sec LB 31 5 V Sample port 02 01 16 2007 15 35 03 31 5 Vbat 21 5 C PORT Post sample acid flush 5 ml 5 sec LB Flush port 49 01 16 2007 18 09 58 31 5 Vbat 23 3 C PORT Pre sample acid flush 5 ml 4 sec LB Flush port 49 Intake flush 10 ml 9 sec LB 31 4 V Flush port 00 Sample 25 ml 20 sec LB 31 5 V Sample port 47 01 16 2007 18 12 03 31 5 ee 22 9 C PORT Post sample acid flush m 5 sec LB Flush port 49 01 16 2007 18 12 45 31 5 vbat i 0 C PORT Pre sample acid flush 5 4 sec LB Flush port 49 Intake flush 10 ml 9 sec LB 31 5 V Flush port 00 Sample 25 ml 20 sec LB 31 5 Vv Sample port 48 01 16 2007 18 14 48 31 5 Vbat 22 9 C PORT Post sample acid flush 5 ml 4 sec LB Flush port 49 PUMPING DATA Sample interval 1 minutes event ml min m1 vbat End of instrument data file Terminate file logging operation now and press any key to continue 00 31 5 V Volume Volume 02 31 5 V 00 31 5 V Volume Volume 47 31 5 V 00 31 5 V Volume Volume 48 31 5 V Volume reached reached reached Volume reached Volume reached reached reached Volume reached Volume reached reached reached Volume reached Figure 8 4 Data Offload File screen 2 of 2 8 After the data is offloaded
21. C End Cap Bolts Plastic Inserts and Screw Holes Three bolt washer assemblies hold the controller housing end cap in place see Figure 2 4 The bolts should be tightened until the lock washers become flattened 20 in Ib A white plastic insert first goes into the screw hole of the controller housing then a flat washer spring lock washer and lastly the bolt All hardware is stainless steel type 316 do not replace with any other type of material A 7 16 nut driver is included in the Toolkit Do not over tighten the end cap bolts cLANE 2 5 RESEARCH LABORATORIES INC Sample Chambers The RAS 100 frame has 8 acrylic blocks of 6 samples for a total of 48 separate sample chambers Each chamber holds a 100ml bag attached to the third turn polycarbonate cap A bleed hole in the sample chamber side allows air to escape during priming Figure 2 6 Block of Sample Chambers Figure 2 7 Sample Bag with Luer Lock Each sample holder is connected in series between the intake head top head of valve and the exhaust head lower half of valve The pump draws water out of the sample chamber that contains the collapsed sample bag The pumping creates a pressure gradient that drives the flow of ambient seawater through the intake into the sample bag After each sample the multi port valve returns to the Home Port 0 sealing the sample in the bag Pumping and Flushing System The valve heads are a HYDEX plastic stator and a Kyn
22. LABORATORIES INC A message will display as a reminder to offload data written to the EEPROM backup during a previous deployment Disregard the message if the data has already been recovered The data offload reminder will be followed by one line of status information and a prompt to proceed with or terminate the deployment a final chance to check the settings prior to deployment Select Y to proceed or N to return to the Main Menu and change settings Caution Deployment will overwrite the EEPROM data backup cache system status 01 16 2007 15 22 25 31 5 Vb 21 8 C PORT 00 Proceed with the deployment Yes No N y Figure 7 25 Data Offload Reminder EaVicLANE 7 15 Low battery output voltage triggers operating warning messages The voltage levels generating the two warning messages are 28 V and 18 V If the main battery is below 28 V a warning message displays the status information and suggests battery pack replacement before deploying the RAS 100 see Figure 7 4 Battery output below 18 V triggers a caution message to replace the battery before running diagnostics If there is deployment data in memory that has not been offloaded a reminder to offload the data before replacing the battery is displayed see Figures 7 5 and 7 6 Proceeding with the Deployment Select Y at the Proceed with the Deployment prompt and the RAS 100 enters a low power sleep LPS mode until the ti
23. McLane Remote Access Sampler RAS 100 gt he SHIPPED FROM McLane Research Laboratories Inc Falmouth Technology Park 121 Bernard E St Jean Drive eee East Falmouth MA 02536 USA How to contact us e E mail mclane mclanelabs com e Fax 508 495 3333 e Phone 508 495 4000 e Internet http www mclanelabs com RAS 100 User Manual Revision History March 2007 Rev B November 2000 Rev A RAS 100 User Manual Table of Contents Chapter 1 lintrodu cei otis s seccsicesecsdevueceseinssecccivenccia sees cays onsnvaodvesccossvanececayeseeosuecrecsaineneene 1 1 McLane RAS 3 48 100 Sampler RAS 100 cceesceseecceteeeeeeceeceneeeceaeeneeaecaeeeeeneees 1 1 Usine IS Manuals Faas decease hhc oa ncn Naxos oa Secrest tcaa ates tials cheat cee au aveo eases AREA 1 1 RASS IOO Overy ei nenea nt ien aieat ts areca eine nae a a ee eas 1 1 RAS 100 Em Dra wines iisiaciscs secangeshec aeia R a a R 1 3 RAS 100 Spe ificationS in ouien a a a ated a E E A 1 4 BA SAV 00 Components ansaa aut terete he ene Bd tah E A E N te Ge at at 1 5 RAS L00 TO UG tea tisatet cotelt at oa eatre set i tematic cenhaces Saas a N 1 6 Contacting McLane Research Laboratories ccccesseceseceseeeesceeseeeaecneeeeeeeeseeeaeenes 1 7 Chapter 2 Mechanical Description sscocssssssssssssssscscsessssssscessssssesssssesssasesssescees 2 1 PE TAIN fe ao tee a saith ant Saheb ila dada ada ba 2 1 Controller Housing ss in i e a Aenea A
24. Offload and Processing explain the process of programming the RAS 100 for deployment and offloading the deployment data RAS 100 Overview The RAS 100 is a time series water sampler that collects up to 48 individual 100m1 water samples in 0 to 50 C water at depths up to 5 500m Deployment duration can be short such as hours or continuous up to 18 months Pre and post sample acid cleaning cycles used throughout the deployment remove bio fouling and other contaminants for more pure samples EaVicLANE 1 1 The RAS 100 user interface controls the multi port valve and displacement pump directs the acid wash cleaning cycles and fluid volume and programs the sampling schedule During the deployment the firmware logs data such as electrical parameters sample timing volume and flow rate This data is offloaded after the RAS 100 is recovered All of the RAS 100 components are mounted inside a 316 stainless steel frame Major components are e Controller housing e Pump assembly e Multi port valve e Sample containers The RAS 100 frame can be deployed as an in line package on a high tension ocean mooring and provides extra mounting space for other instruments Deployment modes include e A bottom tethered array e Ocean floor placement in a stand alone bottom lander e Tethered profiling from a ship as done with a CTD sensor and Rosette sampler RAS 100 Line Drawing The RAS 100 line drawing in Figure 1 1 and the Specificat
25. Optionally Adding Preservative RAS 100 sample bags are available with Luer locking valve fittings or JACO fittings Figure 5 6 Luer Locking Valve Figure 5 7 JACO Fitting Each standard sample bag is shipped with a JACO fitting ferrule short length of tubing and threaded male connector If the ferrule is missing place a new ferrule onto the tubing before use Sample bags with Luer Locking valves do not have a ferrule cLANE 5 5 RESEARCH LABORATORIES INC To install the sample bags complete the following steps 1 From the Manual Operation menu select lt 2 gt Find port 1 2 If adding preservative to the sample bags use a syringe to inject the desired quantity into the bag otherwise skip this step The preservative is drawn into the sample bag prior to the water sample the volume of the sample chamber is approximately 180ml Before adding preservative check the RAS 100 Specifications section in Chapter 1 of this User Manual to ensure chemical compatibility 3 Insert a syringe provided with the Toolkit into the top of the sample bag and pull the syringe out to extract as much air as possible For sample bags with Luer lock fittings close the valve Figure 5 8 Attaching Sample Bag to Sample Cap 4 Attach the sample bag to the sample cap 5 6 cLANE RESEARCH LABORATORIES INC If not using filter holders press down on the syringe in the valve intake to fill the tubing between
26. RAS 100 This manual describes the operation and maintenance of the McLane Remote Access Sampler with 100ml sample bags RAS 100 Designed to autonomously collect time series in situ water samples on an operator programmed schedule the RAS 100 is suitable for deep ocean or coastal water Before first time use of the RAS 100 complete the following e Become familiar with the RAS 100 sampler by reading the Overview section of this chapter and chapters 2 and 3 in this User Manual e Connect the battery or DC power supply e Clean the fluid lines and sample chambers e Install the sample bags and optionally the filters e Release any air trapped in the tubing or filters Priming e Fill the acid flush bag e Install the water flush filter e Establish communications between the RAS 100 and a PC and see Chapter 7 RAS 100 User Interface in this User Manual to program the deployment Using this Manual This manual is organized into several chapters Chapter 2 Mechanical Description and Chapter 3 Electronic Description detail the mechanical and electrical components of the RAS 100 Chapter 4 Maintenance and Storage describes how to clean and store the RAS 100 Chapter 5 Operations describes the steps to prepare the RAS 100 for deployment Chapter 6 Launch and Recovery describes launch and recovery steps including mooring configurations Chapter 7 RAS 100 User Interface and Chapter 8 Data
27. RCH LABORATORIES INC Water Flush Assembly The water flush assembly consists of a 25mm filter holder for a filter with pore size between 3 and 20 microns connected to Home port Port 0 of the multi port valve The filter keeps large suspended particles from entering the pump while the valve is being flushed Water Flush 25mm Filter Holder Port 0 Figure 2 10 Water Flush Filter Holder To prevent sample contamination and reduce accumulated bio fouling the multi port valve flushes resident water from the intake tube and valve heads before each sample is collected The intake tube is further cleaned by a bio fouling acid flush from Port 49 As illustrated by Figure 2 10 the samples and the pump are isolated from each other INTAKE EXHAUST MULTI PORT VALVE MULTI PORT VALVE ONE VALVE IS OPEN AT A TIME ONE VALVE IS OPEN AT A TIME Figure 2 11 Pumping Operation Schematic f KAMSLANE The Port Designation table shown next illustrates how the water flush and acid flush both pump in reverse keep the intake path clear of contaminants Designation 1 Sample 1 Sample 2 Sample 48 Acid Flush Reverse Pump EaVicLANE Serial Number The RAS 100 frame contains a serial number label The firmware menu also displays this information Figure 2 12 RAS 100 Serial Number Label Chapter 3 Electronic Description Controller Electronics Stack The RAS 100 controller is a three board stack mounted
28. Tel 508 495 4000 Fax 508 495 3333 Email mclane mclanelabs com www http www mclanelabs com Software version RAS100_5 c Compi led Jan 5 2007 09 56 29 Electronics S N ML11111 01 EaVicLANE 1 7 EaVic LANE Chapter 2 Mechanical Description Frame RAS 100 components are secured in an electro polished stainless steel welded frame The frame was designed to be an inline component on a high tension up to 2 200 kg ocean mooring and has a built in top bridle and four mooring eyes at the bottom for a chain or cable bridle Each mooring eye accommodates a 5 8 shackle and has an insulator to protect the frame from corrosion The frame provides extra mounting space near the controller housing for other instruments Figure 2 1 RAS 100 Full View cLANE RESEARCH LABORATORIES INC 2 1 Attach zinc or steel anodes to the frame when deploying in a highly corrosive environment Controller Housing The controller housing pressure resistant to 5 500 meters is a cylindrical pressure housing fastened to the frame by two latex rubber insulated 316 stainless steel U bolts The housing holds the battery pack micro controller 3 phase pump motor driver and stepper motor driver A sacrificial zinc anode attached to each end provides galvanic protection from corrosion Figure 2 2 Controller Housing The micro controller which starts automatically when the main or backup battery is connected directs the p
29. ain Menu options and verify deployment settings The valve is aligned to Home Port 0 if this has not already been done Prompts allow the operator to e Offload any existing data that has not already been displayed the warning to offload existing data occurs only if a data file exists and has not been offloaded e Check and reset the time e Create a new schedule or check change an existing schedule The next screens show the prompts displayed after selecting Deploy System McLane recommends using the file capture utility available in Crosscut to log the communication with the RAS 100 including pre deployment bench testing Refer to Appendix A in this User Manual for more information Deployment Initialization First valve alignment with Home Port 0 is confirmed Searching for home port home port located Performing alignment done Alignment confirmed Figure 7 12 Confirming Home Port Alignment If the valve cannot be aligned with Home Port the warning message shown next is displayed the deployment is terminated and the firmware returns to the Main Menu WARNING Unable to find home port This problem must be resolved before deploying the system Begin by checking external connections and exercising the multiport filter valve using the tools found in the Manual Operation menu Figure 7 13 Unable to Locate Home Port Once the valve is aligned the firmware c
30. and then from the PC 4 Connect to and disconnect from the RAS 100 as smoothly as possible Try to prevent repeated intermittent contacts 5 Leave the PC and Crosscut running until the COM cable is disconnected from the RAS 100 Additional Documentation A more detailed and complete description of Crosscut operations can be found in the file crosscut doc which is included with the Crosscut program Crosscut for Win also has a help utility which can be viewed by selecting Help from the menu bar alc LANE Appendix A 5 Appendix A 6 EaVicLANE Appendix B Optional External Temperature Sensor The RAS 100 internal 10K Thermistor temperature sensor can optionally be extended from its standard location on the electronics card to an External 12 4K Temperature Probe via a specially manufactured aluminum block screwed into the top end cap The external temperature sensor is designed to operate linearly between 5 C and 35 C External temperature readings are recorded in RAM and in EEPROM Figure B 1 Thermistor External Temperature Sensor KaMcLA NE Appendix B 1 SEARCH LABORATORIES INC External Temperature Sensor Location in Controller Figure B 2 External Temperature Sensor Installed in Controller Housing Configuring the External Temperature Probe To configure the External temperature probe complete the following steps 1 From the Main Menu type C and type the password configure
31. ar plastic rotor The stator is held stationary while a stepper motor with a 100 1 planetary gear head turns the rotor an optical sensor and slotted disk determine rotor position The motor gear head and optical sensor are in a pressure compensated PVC plastic housing filled with Dow Corning 200 fluid 20cSt Gear Pump A positive displacement gear pump creates the pressure gradient for sampling The pump head is type 316 stainless steel and the motor housing is white PVC plastic The pump head has a user replaceable carbon gears and a replaceable magnet A brushless DC 3 phase servo motor is magnetically coupled to the pump head Hall effect encoders provide feedback to the controller for shaft speed control 2 6 cLAN E RESEARCH LABORATORIES INC Pump Head Figure 2 8 Pump Head Gear and Replaceable Magnet After pumping 100 L of seawater examine the pump head carbon gears and replace if worn A dual head 50 port rotary valve directs seawater to each sample bag The top head has a single intake port and 50 exhaust ports the bottom head has 50 intake ports and a single exhaust port The valve top shows the port numbers and the tubing is also tagged with numbers to further identify the port and valve connections The sample containers and water flush option are connected between the two heads of the valve Only one port is open on each head at one time Home Port Figure 2 9 Multi Port Valve cLANE 2 7 RESEA
32. ater Flushing Time Limit 20 0 0 cece ccceceseceeeceeeeeeeeceeeceeeeneeeeeneees 7 12 Option lt J gt Sample V elOmies see s o ei dedearetial ya seccesare tates aiieincs kee 7 12 Option lt K gt Sample Time Lanit cisco ta lao stiere ay ceates tbe eather aeons 7 13 Option lt L gt Post Sample Acid FIUSH LA cee ecccccssecsteceseceeneceeceeeseecaeceeeseeeeeneees 7 13 Option lt M gt Acid Flushing Volumes sic savscscssevvasavieivsccdaavdacassnevsandviadeaastevdadoncdie 7 13 Option lt N gt Post Sample Acid Flushing Time Limit cee eeeeeeeseeneeneeeeeees 7 13 Option lt P gt Timing Pump Data Period ceeeceecseceseceseeeeeeeesceceaeceeeeeeeenseeeaeens 7 13 Option lt V gt Verify and Proceeds scisnechaccsosectaatlaxssaiabeesdtasbaxdantiananevanentasandiaoele 7 13 TOC 2 EAMSLANE Checking Available Acid Flush Volume cccccssccsseceseceeseceeeeeeaeecseeeeseeseeeeeaeees 7 13 Checking for Event Overlap siciscuiies steed cciiues cakes liad edulis eodauel adios Sal sedas edatae 7 14 Proceeding with the Deployment 4 22ssscegiede desde tees dtadene acca daa eee ads dene anaes 7 16 lt 77 TLIO AND Abels cect liaic tend cea teed tance Mais isle Psy a a dae asia apa th waste 7 17 lt 8 gt Contacting MCL ane sizes sig ienen Gast Ais te tasidene Aas degen aa evade Maes 7 20 Chapter 8 Data Offload and Processing cssccsssscssssscsssccssscccssssccsssscsssssssssssseees 8 1 Appendix A Operating Crosscut and Crosscut for Windo
33. ckle 1 2 Shackle 1 meter length 3 16 Wire Rope or 3 8 Chain 1 2 Shackle 5 8 Pear Ring Figure 6 1 Four to One Bridle Connection If necessary the RAS 100 can be removed from its frame and mounted on a special purpose instrument rig or a bottom lander using longer cables to connect between parts Contact McLane to use this configuration Launch Preparation Preparing and launching the RAS 100 deployment requires the following in order of completion e Connect the battery e Close the end cap e Connect the RAS 100 COM cable e Program the deployment as documented in Chapter 7 of this User Manual e Disconnect the COM cable and attach the dummy plug e Deploy the RAS 100 oie cLANE RESEARCH LABORATORIES INC Remember to remove the intake plug that was used during Priming before deploying the RAS 100 If the plug remains in place during the deployment the RAS 100 will not sample properly Recovery Procedure After the deployment is completed the required steps are e Remove the sample bags for storage and later analysis e Offload the deployment data Steps for offloading the data are included in Chapter 8 Data Offload and Processing in this User Manual Removing the Sample Bags Steps for removing the sample bags are provided below 1 Remove the bag from Port 1 by twisting the sample chamber cap counter clockwise through one third of a revolution do not remove th
34. connect the main battery to the 2 pin connector on the middle board of the electronics stack 3 Install the 9 volt backup battery into the holder 4 Remove the dummy plug from the communications connector Battery connector Figure 3 3 Connecting the Battery 5 Attach the supplied communication cable between the main battery communications connector on the controller housing and the PC serial port cLANE 3 3 RESEARCH LABORATORIES INC Always make and break the COM connection at the RAS 100 bulkhead rather than the PC serial port to avoid crashing the TT8 controller Disconnect the battery before shipping the RAS 100 to prevent electrical system damage during transit Before disconnecting power press CTRL C to return to the Main Menu or Sleep cLANE RESEARCH LABORATORIES INC Chapter 4 Maintenance and Storage Several maintenance procedures should be followed before and after each deployment for smooth operation and long instrument life for the RAS 100 Rinsing the entire instrument assembly with clean fresh water after every deployment is important to prevent corrosion Also clean and flush the valve tubes and pump as described in Chapter 5 Operations Before and after each deployment inspect the following e O rings e Pump gears e Bolts e Fluid lines and sample containers Cleaning and Inspecting the Controller Housing Controller Housing Inspect and if necessary replace the zinc anode
35. e amp Post Sample Flush Exceeds Available Acid Volume 7 13 Figure 7 24 Overlap Reminder cass ieccecyecttteresannsueteanas dane Goteeg eaneeeheciahe dawnGadaeone Gases 7 14 Figure 7 25 Data Offload Reminder ay ra cas csi cavnedasdasactis eencsdey eben coisa aotendnenes 7 15 Figure 7 26 Deploy System ssssssessessssseessessessresseossesoesseossessesseossessrsseesessesseesseses 7 16 Figure 7 27 Offload Display Data File ccssstossiasavsaiasteadectianted eas ister siataediegbaxdextaactales 7 18 Figure 7 28 EEPROM Data Backup Cache ceceeccescsseeseeeseeeseeeeceseceaecneeeneeeneees 7 19 Figure 7 29 McLane Contact Inform att once s sseijatisescciecssntdsacds sci msoasavavaadencde oideaias 7 20 Figure 8 1 Offload Display Data File Menu ccceecceseceseeereeeeeeeeceaeeneeeseeeeeeeeaees 8 1 PSS Dass ei oe eee oes Seatac locas Statute E ied vee Gaudet EN vanes 8 2 Figure 8 3 Data Offload File screen 1 of 2 sist ans ietieeeetagelainee teiioss 8 2 Figure 8 4 Data Offload File Screen 2 Of 2 cecceececcceessecsteceeeceeeeeeseeceteceeeeeeeeenneees 8 3 Figure B 1 Thermistor External Temperature Sensor ecceeceeeeceteeneeeteeeeeeeeeeaees B 1 Figure B 2 External Temperature Sensor Installed in Controller Housing B 2 Figure B 3 System Configuration Menu Temperature Configuration B 2 LOF 2 EaVicLANE Chapter 1 Introduction McLane RAS 3 48 100 Sampler
36. e bag by pulling on the cap or the bag s intake tube Disconnect the tubing at the bottom of the acrylic sample chamber to reduce the force required to remove a full sample pressure on the bag can cause some of the sample to flow back through the intake tube valve potentially contaminating the sample 2 Lift the cap gently and using the thumb and index finger firmly grasp the sample bag just below the intake tube 3 Gently pull the bag out of the sample chamber by gripping the bag just below the intake tube 4 Disconnect the sample bag from the cap and install a 1 4 28 threaded female cap on the tubing Store the sample safely and replace the cap on the sample chamber for Luer Lock bags close the valve and install the cap 5 Repeat steps 1 4 for all sample bags 6 Reassemble the RAS 100 and perform any necessary maintenance see Chapter 4 Maintenance and Storage in this User Manual 6 4 EaVicLANE Chapter 7 RAS 100 User Interface This chapter describes the RAS 100 firmware menus commands and screens Power Up Sequence Connecting the RAS 100 battery automatically loads the TT8 RAM A screen prompt displays to set the time and date press ENTER to accept the default time and date If the time and date screens do not display and the battery has been connected for some time press CTRL C three times to wake the RAS 100 from Low Power Sleep LPS If the screens still do not display conf
37. ed Syringe on Valve Intake eee eeceeseeeeeeeeceeeceneeneeeneeeeees 5 4 Figure 5 5 Removing Filter Holder 1 0ps 4cetvcnwe cuts neh aces eee 5 4 Figure 5 6 Luer Locking Valve ecalaarasotieccugusteratvectaauneeutadnetaute eared aane 5 5 Figure 5 7 JACO FINE nikoan o igea a e a a a aeai 5 5 Figure 5 8 Attaching Sample Bag to Sample Cap sssessesssssssseseesesseseesesseesessesresesse 5 6 Figure 5 9 Reassembling Filter Holder saisearacsaudascsoussasaveavienevtenahansevansagu sesbanaedevrevantas 5 7 Figure 5 10 Intake Plt ccs iiini ini atai a aatia kiaia 5 8 Figure 5 11 Water Flush Filter Holder scce nssecsis cca seat onniasansnadtsdaiadloniatnsnests 5 11 Figure 6 1 Four to One Bridle Connection ssssssesessessssseesessestsesstseesrsseeresseseesesse 6 2 Fig re 7l M n Win a e eee ace teases s ana eee EE AEE E E ee a 7 1 Fig re 7 2 Set Meerin sy a E a a e E E E 7 2 Figure 743 la PHOSUICS ere ereen a a a Sean Eea A Ria 7 2 Figure 7 4 Low Battery Voltage ss sseseseseessessesseessessresresseestesresstessesersseessessrssressesse 7 2 Figure 7 5 Critically Low Battery Voltage s cscscscesscascuscesscsscesnseatecsssidsendessrsecsesddeneees 7 3 Figure 7 6 Critically Low Battery Voltage Offload Data eee ceeeeseeseeereeeeeeees 7 3 Figure 7 7 Manual Operation Men s lt eesccdsccssctashsveveass cpecttouscvasecarsestashceceeusndgecss 7 3 Figure 7 8 Run Pump Forward ig scacd ci cea ais sonk etic aa ispacd acta
38. eplacement ccis cartes eis estat a E EE Se ees 4 2 BACKUP Batter yinin a i E EE dee lasy teats 4 3 OCA A EEE TEE A E 4 3 Chapter S Operations asics cedees o5ceecacssves cess du casvwacveaneckswc essi bo esso devoa scdeos sdevaccesavasevencsees 5 1 Cleaning the Fluid Lines and Sample Chambers 0 cccceccecssecseceteceeeeeeeeeeeeeeseenes 5 1 Priming the RAS OO iiinn a a abate eate ede denen 5 3 Step l Preparing to Pime rnein nicnn an serosa sans ote a a E a 5 3 Step 2 Installing the Sample Bags and Optionally Adding Preservative 5 5 Step 3 Filling Sample Chambers with Water and Reverse Pumping 5 8 Step 4 Adding Acid Flush to Sample Chamber Number 49 000 eeeeeeeeeeereenee 5 10 Step 5 Installing the Water Flush Filter cscdivatyeienstelscacveeniicensisysaede 5 11 Instrument Current Consumption s sesssssssesseeseesseeseseesseestesesstestesrrsstesresrrssessesees 5 12 Example of Determining Battery Life RAS 100 One Year Deployment 5 13 KAMcLANE TOC 1 Chapter 6 Launch and Recovery sscsccsssssssssssssssssscsssssssssssssssesssssesssssssssssssssasesens 6 1 Attaching to A NIGOTIN Gy lt s3xcs4acdneieaiecsbsesiesaiasvbucssntdutesdaule A REE AEE EE a Ea A 6 1 Simple Morin tcann a a a a haat 6 1 Panel PLS Para tt O ti mescita oietan aE AEEA AA EE AN ERA TENA 6 2 Recovery Procedure maiie a a a a E A E 6 3 Removing the Sample Bags sssssssseesssseesseesresee
39. fies the time limit for the samples The maximum time limit is determined from the selected flushing volume and the fixed minimum flow rate of 20 ml min Option lt L gt Post Sample Acid Flush This option enables or disables the post sample acid flush Option lt M gt Acid Flushing Volume This option specifies the amount of water flushed through the water port before sampling Option lt N gt Post Sample Acid Flushing Time Limit This option specifies the post sample acid flush time limit The maximum time limit is determined from the selected flushing volume and the fixed minimum flow rate of 20 ml min Option lt P gt Timing Pump Data Period This option establishes the instantaneous flow rates and volumes for each sample that are logged in the data file at a user selectable interval Option lt V gt Verify and Proceed Select this option when finished changing the pumping parameters Prompts will display to change parameters that are in conflict with other parameters Checking Available Acid Flush Volume If more acid is requested than available a warning message displays after V is selected The total acid volume available is 480ml the amount of acid in the sample bag Press N to change the parameters and Y to continue with the deployment WARNING The total acid requested for pre amp post sample flushes exceeds the total acid volume available Proceed with the deployment Yes No N n
40. gnteanceageseraardntaats 2 2 Figure 2 3 Controller Housing Top End Cap with O Ring Seals eee 2 3 Figure 2 4 Controller Housing End Cap Bulkhead Connectors ccceeeeereeetees 2 4 Figure 2 5 Aligning Cable Connectors with Thumb Bump ceseeeeeseeereereeeeees 2 5 Figure 2 6 Block of Sample C hanibers osc 6a saseis esisustcasiy avesiae aeoanase severe as 2 6 Figure 2 7 Sample Bag with Luer Lock iycccc siyechectscciende ecoscaaceahonde eo ceematosencisneov 2 6 Figure 2 8 Pump Head Gear and Replaceable Magnet eceeseeeceseeseereeeeeeeeeeeees 2 7 Figure 2292 Multi Port Valve crioed niiina Atadiad arieiean te 2 7 Figure 2 10 Water Flush Filter Holder sisisisscsaeiststovsacsasiadusttavvaasaveevesd pdecsdansaaeraeiseeas 2 8 Figure 2 12 RAS 100 Serial Number Label eee eceeccesecetecneeeeeeeeeeeeeeaecnaeeneeees 2 10 Figure 3 1 RAS 100 Controller Stack oss 2 andar vaikavetesdaed dustiades wiapedunoanmarconsedeasecns 3 1 Figure 3 2 Three Boards of Electronics Stack ce ceeccesceseeereeeeceeecesecneeeseeeeeeeeeeaees 3 2 Figure 3 3 Connecting the Battery isi51 tscrsuestecs taedsnstaadsetacneetees iste tiavaenicasiaaetiaeteteans 3 3 Pioure 41 Manal Operation sennen eeens ess atk So tiie nae ee 5 1 Figure 5 2 Sample Chamber 1 OW cr acutestseacl seycachende ces capaeeeude aes waaeneaatadeeascanastl ens 5 3 Figure 5 3 Sample Chamber Top Without Frit 6 0 ose csageustasiciceumecndenwntesens 5 3 Figure 5 4 Water Fill
41. hecks to see if a data set that has not been offloaded exists in memory A data set exists in system memory that has not been offloaded Existing data will be lost if a deployment schedule is defined Continue Yes No N Figure 7 14 Data Set Exists in Memory Select Yes or No as appropriate The firmware cannot detect whether Capture to File in Crosscut is active during the offload operation Confirm that Capture to File is ON during offload Next the real time clock date and time display To change the time select Y If the time is correct select N Press any key to continue Clock reads 01 16 2007 15 21 35 Change time amp date Yes No N Figure 7 15 Real Time Clock If there is deployment data still in memory the message below is displayed Select Y to delete the deployment data N to return to the Main Menu Existing deployment data file will be erased Continue Yes No N y Figure 7 16 Previous Deployment Data in Memory If a schedule is already in memory the message below is displayed Select Y to delete the current schedule or N to keep the schedule Enter new deployment schedule Yes No N y Figure 7 17 Enter New Schedule Enter the number of events to program Enter the number of events to program 0 to 48 48 Figure 7 18 Enter Number of Events to Pr
42. ile in the Name field or use the TAB and ENTER keys to select the directory and enter the name in the Name field 6 Once the capture is started everything that appears in the Crosscut window whether from the instrument or the keyboard is written to the file Run the offload utility and the data unpacked from the flash card will be captured in the named log file Note that Crosscut always overwrites the information in an existing log file 7 Terminate file logging by selecting CommPort and Capture to File again or by typing ALT Z 8 To exit Crosscut select File from the menu bar and Quit from the submenu or type ALT Q The format of the captured data file is ASCII text use the extension TXT for the file name ASCII text files can be loaded and edited by all of the common word and text processors Crosscut assigns a default file name of CAPTURE TXT to log files The operator can as described above change the name before logging begins That name becomes the default during that Crosscut session Alternatively the file can be renamed once captured to the hard disk of the PC In either case Crosscut does not append each log file must eventually have a unique name and path or it will be overwritten Using Crosscut for Win Crosscut for Win is a Windows based program that runs ona PC Crosscut for Win will run without modification or difficulty under all currently available versions of Windows
43. inally 100 ml acid reservoir the actual volume is assumed to be 480 ml divided by the number of samples in the schedule Option lt F gt Acid Flushing Time Limit This option specifies the acid flush time limit The maximum time limit is determined from the selected flushing volume and the fixed minimum flow rate of 20 ml min Option lt G gt Acid Exposure Time Delay This option specifies a time delay during which the acid will be left standing in the valve intake This allows the acid sufficient time to act upon any growth that may be present The acid will be removed by the water flush If the acid flushing volume is zero the delay cannot be set to a value other than zero and will not be executed The exposure delay is automatically set to zero when the acid flushing volume is set to zero Option lt H gt Water Flushing Volume This option specifies the volume of water to flush through the valve before taking a sample This is done to clear out any debris in the valve and intake hose and to ensure that the sample is taken entirely from current ambient water Option lt I gt Water Flushing Time Limit This option specifies the water flush time limit The maximum time limit is determined from the selected flushing volume and the fixed minimum flow rate of 20 ml min Option lt J gt Sample Volume This option specifies the volume of water to be pumped for each sample Option lt K gt Sample Time Limit This option speci
44. ion for example from Port 3 to Port 4 Option lt 4 gt Next port retreat This option moves the valve backwards one port position for example from Port 3 to Port 2 Option lt 5 gt Run pump forward This option pumps 100 ml of water at 75 ml min in the forward direction The pump can be stopped at any time before the operation is complete by pressing CTRL C Each row of the display shows the pump speed control value the instantaneous pump speed in Hertz the average pump speed in Hertz the cumulative volume pumped the instantaneous flow rate in ml min and the elapsed time in seconds 1676 h 401 I_Hz 100 A_Hz 1 4 ml 83 8 ml min 1 secs 1582 h 286 I_Hz 171 A_Hz 2 7 ml 78 8 ml min 2 secs 1695 h 183 I_Hz 217 A_Hz 4 0 ml 76 3 ml min 3 secs Figure 7 8 Run Pump Forward Option lt 6 gt Run pump reverse This option pumps 100 ml of water at 75 ml min in the reverse direction The pump can be stopped at any time before the operation is complete by pressing CTRL C Option lt 7 gt Run pump programmable This option allows the operator to enter the volume time limit and direction for pumping The pump can be stopped at any time before the operation is complete by pressing CTRL C The various software features controlling and safeguarding pump operation are in effect whenever the pump is running lt 4 gt Sleep This option places the RAS 100 in Low Power Sleep LPS mode to conserve batte
45. ions List that follows illustrate the major components and mechanical design of the RAS 100 sampler Containers Pel Te Y T Tl Tr qj Pa TE UL AE TE Uk I Pea Tr UL TE UE ah kL o L Hob H L i L ob JL a Multi port Valve T Controller Acid Reservoir Housing Figure 1 1 RAS 100 Line Drawing Overall View RaVicLANE RAS 100 Specifications Dimensions Weight Approx Multi port Valve Sample Bags 48 Pump Controller Frame Operations Height Width Length body In air sample containers empty In air sample containers full In water Number of Ports Material Drive Positioning Size Material Flow rate Type Drive Housing Material Power Supply Power consumption Communications Material Structure bridle configuration Frame amp bridle eyes Max in line tension Maximum depth Min Max deployment time Operating temperature 165 cm 64 9 in 43 cm 16 9 in 43 cm 16 9 in 75 kg 165 lbs 86 kg 190 Ibs 42 kg 93 lbs 50 ports 48 samples HYDEX plastic valve stators Kynar plastic rotor High torque stepper motor 100 1 planetary gear head Optical sensor with slotted disk Approximately 100 ml Metalized polyethylene lined or Tedlar 75 ml min fixed rate 3 error Gear pump Brushless 3 phase DC motor Aluminum 6061 T6 hardcoat anodized 31 5 VDC Alkaline battery pack 3 100 mAh 1 year deployment Serial RS 232 316 electro polished stainles
46. irm the COM port connection and communication protocol settings 9600 baud 8 data bits 1 stop bit and no parity The Main Menu Operating the RAS 100 The Main Menu provides the user interface to control all RAS 100 operations To select an option type the appropriate alphanumeric character and press ENTER McLane Research Laboratories USA _ 100 ml Remote Access Sampler Version RAS100_5 c S N ML11111 01 Main Menu Tue Jan 30 12 00 05 2007 Port 99 lt l gt Set Time lt 5 gt Create Schedule lt 2 gt Diagnostics lt 6 gt Deploy System lt 3 gt Manual Operation lt 7 gt Offload Data lt 4 gt Sleep lt 8 gt Contacting McLane Selection Figure 7 1 Main Menu cLANE 7 1 RESEARCH LABORATORIES INC lt 1 gt Set Time This option sets the real time clock RTC McLane recommends that the RTC be set during the power up sequence When the RAS 100 is powered on the clock defaults to January 1 1970 00 00 00 The operator can set the clock to any date and time in the allowed range and the count will continue from the new value Clock reads 01 01 70 00 48 21 Change time amp date Yes No N y Note Year 2000 100 2001 101 etc Enter correct time 01 01 70 00 48 24 11 15 98 20 30 55 Clock reads 11 15 98 20 30 55 Change time amp date Yes No N n Figure 7 2 Set Time lt 2 gt Diagnostics Diagnostics is a scrolling status display including date ti
47. ithout filter holders is also provided Complete these steps in order e Step 1 Preparing to Prime e Step 2 Installing the sample bags and optionally adding preservative also installing the 25mm filters if filter holders are installed e Step 3 Filling the sample chambers with water reverse pumping on each valve e Step 4 Filling the acid flush bag for Port 49 e Step 5 Installing the water flush filter and priming Home Port Figure 5 2 Sample Chamber Top Figure 5 3 Sample Chamber Top Without Frit Step 1 Preparing to Prime To prepare for priming complete the following steps 1 Power up the PC start Crosscut and then plug the RAS 100 COM cable into the PC serial port 2 Connect the RAS 100 battery if this is the first use of the RAS 100 since shipment the battery will be inside the controller housing but not connected 3 Close and seal the controller housing Priming is a wet process Ensure the electronics housing is sealed to protect the electronics cLANE a RESEARCH LABORATORIES INC 5 4 4 From the RAS 100 Main Menu select lt 3 gt Manual Operation 5 From the Manual Operation menu select lt 1 gt Find port home 6 Place a large syringe provided in the Toolkit full of water on the valve intake Figure 5 5 Removing Filter Holder Tops 7 Ifusing filter holders remove all filter holder tops cLANE RESEARCH LABORATORIES INC Step 2 Installing the Sample Bags and
48. me battery voltage in Vb temperature in Celsius and valve status A sample diagnostics display is shown below Type X or CTRL C to exit from Diagnostics and return to the Main Menu Toggle the scrolling on and off without exiting by pressing any other alphanumeric key 01 30 2007 20 50 03 32 1 Vb 25 2 C PORT 00 01 30 2007 20 50 04 32 1 Vb 25 4 C PORT 00 01 30 2007 20 50 05 32 1 Vb 25 4 C PORT 00 01 30 2007 20 50 06 32 1 Vb 25 2 C PORT 00 01 30 2007 20 50 07 32 1 Vb 25 3 C PORT 00 Figure 7 3 Diagnostics Low battery voltage triggers operator warning messages If the voltage of the main battery pack is below 28 V a message displays during the exit from the diagnostic routine suggesting battery replacement before deployment Battery voltage is abnormally low cCheck replace main battery pack before deploying system Press any key to continue Figure 7 4 Low Battery Voltage If the voltage of the main battery pack is below 18 V only one status line will be printed the diagnostic routine will terminate and the program will return to the Main Menu Main battery is extremely low and should be replaced before running diagnostics Figure 7 5 Critically Low Battery Voltage If a critically low battery is detected and a data file exists in memory that has not been offloaded an additional warning will be displayed before returning to the Main Menu A data
49. me of the first scheduled event If conducting a bench test leave the communications cable attached to the RAS 100 electronics to observe the operation of the instrument in the Crosscut window do not touch the keyboard During an actual deployment disconnect the communications cable from the controller housing Reconnect the dummy plug to the communications connector on the controller housing and deploy the RAS 100 Remove communication cable and attach dummy plug System is ready to deploy lt 01 16 2007 15 22 28 gt waiting for event 01 of 48 01 16 2007 15 30 00 01 16 2007 15 22 30 Sleeping Figure 7 26 Deploy System The RAS 100 program enters a monitor mode where no further user entries are allowed except CTRL C 7 16 cLANE RESEARCH LABORATORIES INC lt 7 gt Offload Data This option works with the Capture to file feature of Crosscut After recovering the RAS 100 and re establishing the communications link with the PC select lt 7 gt Offload Data to process the binary data and offload to a disk drive Offload data before disconnecting the battery otherwise the data will be lost EaVicLANE 7 17 To use the Offload Data option complete the following steps 1 Start Crosscut on the PC The PC should be on and Crosscut running before connecting to the RAS 100 electronics Failure to observe this caution can crash the program Only data stored in the EEPROM can be rec
50. of the frit Reassemble the filter holder 10 Remove the syringe and place the intake plug on the valve intake 11 Program the deployment refer to Chapter 7 and put the RAS 100 to sleep 12 Remove the intake plug from the valve intake just prior to launching cLANE 5 11 RESEARCH LABORATORIES INC Remove the intake plug just prior to launching the RAS 100 so that the pump can operate Instrument Current Consumption The values for pumping or moving the valve include the current drain of the controller which is awake during valve rotation and pumping Using the instrument current consumption values listed next the operator can estimate battery life from the proposed deployment schedule An example of this procedure for a one year deployment is shown next Controller unit in low power mode 0 30 mA il Moving the valve from one port to the next 0 23 mAh 2 7 seconds Example of Determining Battery Life RAS 100 One Year Deployment To determine Battery life for a RAS 100 deployment the Pumping 2 minutes per port E iil ERED Running the pump 1 0 hour ee rr a a Oa 50 ports x 0 23 mAh 11 5 mAh Subtotal 17 5 mAh Total Current Consumption 192 3183 17 5 3392 5 mAh In this example the total energy consumed is also less than the 10 000 mAh capacity of the battery and the proposed RAS 100 deployment plan will not exceed battery life EaVicLANE 5 14 EaVicLANE Chapter 6 Launch and Rec
51. ogram Entering or Changing a Pumping Schedule The Schedule Menu is displayed next and allows creation or modification of an event schedule To enter the start data and interval from the Main Menu select lt 2 gt Schedule Menu and then select lt 2 gt Enter start date amp interval Schedule Menu lt l gt Enter each event time lt 2 gt Enter start date amp interval lt 3 gt Enter start date amp end date lt M gt Main Menu Selection 2 Figure 7 19 Schedule Menu Enter the start date and time for the RAS 100 to begin pumping the first sample Then enter the interval between the start time of one sample and the start time of the next sample Enter START date and time 01 16 2007 15 21 45 1 16 07 15 300 Enter interval Days 0 to 365 O Hours CO to 23 0 Minutes 0 to 59 3 Figure 7 20 Pumping Start Date amp Time Date and time information can be separated by SPACE or entered using the display format MM DD YY HH MM SS cLANE TR RESEARCH LABORATORIES INC A schedule of the event times will appear on the screen in blocks of 16 events Verify the dates and times and respond to the Modify an event prompt To review the next remaining block press any key Event Event Event Event Press 4 4 an Schedule Verification 01 16 2007 15 30 00 01 16 2007 15 33 00 lof 48 2 of 48 7 of 48 8 of 48 y key to continue
52. on and print the data file to the screen to also create a permanent log file use the Capture to file feature within Crosscut After running the Offload Data option confirm that data is successfully offloaded by checking the Crosscut files before disconnecting the battery data is erased when the battery is disconnected To offload the deployment data complete the following steps 1 Place the RAS 100 in a dry area power on the PC and start Crosscut Powering on the PC and starting Crosscut before connecting the RAS 100 serial cable to the PC prevents a possible crash of the RAS 100 firmware 2 Remove the dummy plug from the RAS 100 COM connector 3 Connect the RAS 100 serial cable to the PC 4 From the Main Menu select lt 7 gt Offload Data Offload Display Data File Tue Jan 30 12 00 50 2007 lt l gt Display ALL data lt 2 gt Display event summary data lt 3 gt Display pump data lt 4 gt EEPROM data backup cache lt M gt Main Menu Selection 1 Figure 8 1 Offload Display Data File Menu 5 From the Offload Display Data File menu select lt 1 gt Display ALL Data The screen shown next displays Start the capture file now Then press any key to start the transfer The data file will remain in memory and is not erased by this offload procedure Figure 8 2 All Data 6 Start Capture to file within Crosscut 7 Press any key to offload the data
53. ops_and_crosscut html Contact McLane or Onset Computer www onsetcomp com for additional information First Time Crosscut Use To use Crosscut for the first time complete the following steps 1 Place the six Crosscut files from the disk provided in the Toolkit into a directory on the PC or create a new directory Alternatively create a Crosscut directory and place the six Crosscut files there If you operate Crosscut from the directory that contains the files changes to the path are unnecessary 2 Type crosscut at the DOS prompt or click on the Crosscut icon to run the program a short cut can also be created from the desktop Do not connect the PC to the TT8 yet alc LANE Appendix A 1 3 Select CommPort from the menu bar at the top of the Crosscut window and then select Port setup from the submenu use a mouse or the keyboard combination ALT P 4 Use the mouse or TAB and arrow keys to set the COM port being used to 9600 baud 8 data bits 1 stop bit no handshaking and no parity 9600 8 N 1 The copy of Crosscut on the floppy disk should have these values preset 5 Click OK to store these values in the configuration files and they will be used whenever Crosscut is started again 6 Connect the RAS 100 serial cable to the PC Editing the Registry to Enable Crosscut 1 On the desktop of a PC running a recent version of Windows click START and select RUN from the pop up menu 2
54. otential damage by large particles Sample Bags Each sample bag is connected in series between the intake head top half of valve and the exhaust head lower half of valve The pump draws water out of the sample container in which the collapsed sample bag is mounted This pumping creates a pressure gradient that drives the flow of ambient seawater through the intake and into the sample bag After each sample is taken the multi port valve returns to the Home Port 0 sealing the sample in the bag RaVicLANE 1 5 RAS 100 Toolkit Each RAS 100 comes with a Toolkit that contains tools software and spare parts including 1 6 9V Backup battery Wrenches screwdrivers and hex drivers sized for the RAS hardware Spare o rings nylon and stainless steel screws and bolts Spare polyurethane tubing Communications cable for serial port connection to a PC CD with the Crosscut software and documentation Figure 1 2 RAS 100 Toolkit KAMSLANE Contacting McLane Research Laboratories McLane Research Laboratories can be accessed via the Web at http www mclanelabs com or reached by email at mclane mclanelabs com McLane contact information also displays in the firmware Engineers are available by phone at 1 508 495 4000 from 1300 to 2200 GMT 0800 to 1700 Eastern Standard Time McLane Research Laboratories Inc Falmouth Technology Park 121 Bernard E Saint Jean Drive East Falmouth MA 02536 USA
55. overed after such a crash 2 Within Crosscut specify a Capture file for the offloaded data 3 From the Offload Display Data File menu select option lt 1 gt lt 2 gt or lt 3 gt to display recorded data as a scrolling ASCII text file on the screen Option 1 shows all the data in a single scrolling display Options 2 and 3 display the two main portions of the data file separately The Offload Display Data File Menu is shown next Offload Display Data File Tue Jan 30 12 00 50 2007 lt l gt Display ALL data lt 2 gt Display event summary data lt 3 gt Display pump data lt 4 gt EEPROM data backup cache lt M gt Main Menu Selection 1 Figure 7 27 Offload Display Data File Option 4 accesses a back up copy of the most critical data which is written to non volatile memory during deployment In the unlikely event of complete battery failure or a serious firmware malfunction the EEPROM backup provides limited data recovery An example of Option 4 output is shown below Note that only the most critical data for sample processing is included During deployments a backup copy of the most important information in the datafile is also written to EEPROM The EEPROM data cache contains the start and stop time for each sampling event that occurred the volume of water pumped through the falter during the event and the port number of the valve To copy the EEPROM cache file to a disk file S
56. overy Attaching to a Mooring The RAS 100 can be deployed on different mooring types for deployment durations of up to 18 months As a stand alone mooring the RAS 100 requires 140 kg of positive flotation As part of a complex mooring array the RAS 100 can be used as a bottom tethered or floating in line instrument For profiling applications in depths less than 5 500m the RAS 100 can be lowered from a ship by winch Simple Mooring An example of a simple RAS 100 mooring setup is below Mooring components are listed from top to bottom sea floor Top e 250 kg of positive flotation 140 kg for the RAS 100 sampler and 110 kg for the other mooring hardware e A3mpiece of 5 8 stainless steel chain attached to the top eye of the RAS 100 frame with a 3 4 shackle through the white Acetal insert insulator e A four to one bridle connection see Figure 6 1 attached to the bottom mooring eyes of the RAS 100 frame through the white Acetal inserts insulators e 3mof 5 8 stainless steel chain e An acoustic release e A 200 kg weight Bottom If possible do not deploy or recover the RAS 100 through an oil slick oil can damage the pump and valve If this condition is unavoidable pour clean fresh water over the spot where the RAS 100 will enter or break the water surface through the slick and rinse the instrument thoroughly after recovery cLANE 6 1 RESEARCH LABORATORIES INC lt ___ 5 8 Sha
57. rogrammed sampling settings and controls the pump and valve actions The RAS 100 firmware on the microcontroller allows testing entry of deployment schedules and offloading recovered data Top and Bottom End Caps The electronics and battery pack are fastened to the inside of the top end cap the end cap with bulkhead connectors Both top and bottom end caps seal the controller housing against water intrusion with a face o ring and a radial o ring with a radial back up ring Face O Ring Figure 2 3 Controller Housing Top End Cap with O Ring Seals End Cap Bulkhead Connectors The bulkhead connectors in the RAS 100 end cap have a different number of pins for each connection Multi Port Valve Pump and Com Port Additionally the connectors are labeled P Pump V Multi Port Valve and C Com Port cLANE a RESEARCH LABORATORIES INC lt Bolt Lock Washer Flat Washer i Plastic Insulator Zinc Anode External Temperature Sensor Option Valve Connector labeled V Pump P Connector ommunication labeled P Connector labeled C Figure 2 4 Controller Housing End Cap Bulkhead Connectors The thumb bump on the cable connector should always be aligned with the thick pin on the bulkhead connector Figure 2 5 Aligning Cable Connectors with Thumb Bump Start the PC and Crosscut before connecting the RAS 100 COM cable to the P
58. ry power When the RAS is not running a schedule LPS automatically triggers after 20 minutes of inactivity Prior to LPS the current time will display During LPS the firmware wakes every 20 minutes to check status display the time and then return to LPS This is also the operation mode after the last event of a schedule is completed and during the intervals between events To wake the firmware and return to the Main Menu press CTRL C three times 01 30 2007 12 29 14 Sleeping Enter lt CTRL C gt now to wake up Figure 7 9 Low Power Sleep lt 5 gt Create Schedule This option allows creation of a sampling schedule in advance of the actual deployment The schedule consists of preprogrammed dates and times that will trigger pumping events and specifies the number of samples to be taken from 1 through 48 Scheduling is Year 2000 compliant and properly accounts for leap years Creating a new schedule makes data in memory inaccessible Confirm that the real time clock and the scheduled events refer to the same time zone e g GMT or local time cLANE 7 5 RESEARCH LABORATORIES INC Select lt 5 gt Create Schedule and enter the number of desired sampling events This prompt will be preceded by warnings if previous deployment records a data file or a deployment schedule exist Enter the number of events to program 0 to 48 48 Figure 7 10 Create Schedule Schedule Menu
59. s prior to each deployment Ensure that the insulated bushings are installed properly and that all hardware is 316 stainless steel Keep hard objects such as tools and shackles from coming in contact with the controller housing as scratches that penetrate the anodized aluminum hard coating will localize galvanic action and can lead to deep crevices or pits O Rings The RAS 100 ships with a set of o rings in the end caps at both ends of the controller housing Each set includes 3 o rings 2 round section o rings BN70 2 244 BN70 2 240 and one back up ring BN90 8 240 O rings must be installed properly and kept in place at all times Positioning the larger o ring 2 244 in the axial groove provides a seal against the face end of the pressure housing Fit the smaller o ring 2 240 and the backup ring 8 240 in the radial groove Position the backup ring on the low pressure side of the radial groove toward the interior of the controller housing The backup ring concave side faces toward the round o ring The accompanying o ring is seated on the concave side high pressure side of the backup ring Inspect o rings for signs of wear and the presence of any foreign material which can cause leaks Look for small cracks and feel for grit sand or hair O rings should be cleaned with alcohol and lubricated with a thin coating of o ring grease as necessary KAMcLANE 4 1 When inserting the end cap into the controller housing ensure that
60. s steel In line mooring weldment 4 in line 19 mm diameter insulated 2 300 kg 5 000 Ibs 5 500 meters 5 minutes per sample 18 months 0 to 50 C Electronics tested to 10 C EaVic LANE RAS 100 Components The RAS 100 components are further described below Component Description Controller Housing The controller housing is a sealed pressure housing tested to a depth of 5 500 meters The housing contains a battery pack a micro controller a 3 phase pump motor driver and a stepper motor driver for the multi port valve Communications The communication link between the RAS 100 and a PC is a standard 3 wire full duplex RS 232 connection Pump Assembly A positive displacement gear pump draws seawater in the sample containers at a fixed rate of 75 ml min small disc filters are optional A brushless DC 3 phase servo motor is magnetically coupled to the pump head The motor is in a pressure compensated housing filled with Dow Corning 200 fluid 5 cSt Hall effect encoders provide feedback to control shaft speed Multi port Valve A multi port valve directs the seawater to the sample bags The multi port valve can be programmed to flush old water from the tubes and valve before each sample is collected to help prevent sample contamination and reduce accumulated bio fouling A small 25 mm disc filter with a pore size from 3 to 20 microns is placed in line with the water port to protect the valve from p
61. set exists in memory that has not been offloaded Offload the data now before replacing battery or the data may be lost Figure 7 6 Critically Low Battery Voltage Offload Data The firmware can detect only that the Offload Data option was successfully executed from the Main Menu and cannot check the successful data file logging by the terminal emulator Confirm that offloaded data is accurate before disconnecting the battery lt 3 gt Manual Operation The Manual Operation option allows direct control of the valve and pump Port 99 indicates an unknown valve position The valve must first find the Home Port Manual Operation Tue Jan 30 12 04 13 2007 Port 99 gt Port 99 lt l gt Find port home lt 2 gt Find port J lt 3 gt Next port advance lt 4 gt Next port retreat lt 5 gt Run pump forward 100 m1 lt 6 gt Run pump reverse 100 ml lt 7 gt Run pump programmable lt M gt Main Menu Selection Advancing belt to next sample position done Figure 7 7 Manual Operation Menu cLANE 7 3 RESEARCH LABORATORIES INC Option lt 1 gt Find Port home This option moves the valve to a reference position located at the water flush port also known as the Home Port or Port 0 Option lt 2 gt Find Port This option moves the valve to a specified port Option lt 3 gt Next port advance This option moves the valve forward one port posit
62. sseessesresstesesrrsseessessesseessessesseeesee 6 3 Chapter 7 RAS 100 User Interface eessoessocsssecssocesocesoosssocsssecssocesocesoosssoesssecssocesooseo 7 1 PowerUp Seguente aeneis a EEE AE a aE A EE E SN 7 1 The Main Menu Operating the RAS 100 c ccc cescecsseceseceeeeeeseeeseeceeeseneeeaeeeaeeees 7 1 ON Set Time aio ads sev ees aucune Sapna thai ca dec aaa Va dea as avec tea ke an ones Ne 7 2 2 TIIASTIOSEICS saaprasardasazetnentaddczasaaaesaesudatanoiaiswsias E sansa EENE 7 2 lt 3 gt M n al per ath Ot sedinane aa a a a yids 7 3 Option lt 1 gt Pind Port NOME sireiticsicnnuricisne otani a e a 7 4 Option a2 gt Find Portsea ene a anea a a a a a a or aS 7 4 Option lt 3 gt Next port advan Cer ccace tise es adie ee seuss ould sbatuceass oad skinner ee seed 7 4 Option lt A gt NEXt POF TE Cal izstesdtes adncerandsns sprcsasdsnaccemaavesteatictounlasacestaphadienaagsevianst 7 4 Option lt 5 gt Run pump forward s3 c sseiedssscciiasasdeeiiahectebtemuateneiniecuhten Gadscts 7 4 Option lt 6 gt Run pump reverse 2 2615 csaracss cava tdnctsascalasdelscoscatsgceaneeencs oeccncasameree eres 7 4 Option lt 7 gt Run pump programmable jas soe eves occiele soca veda daddaanevtsecesuiedducesy cece 7 4 A Sleeping ac duca es iansuugs cuaecsti aes aude agua gvanee a eadentecia ugenentuatiedinade adeadunas 7 5 lt 5 Create Schedule seas imide in ah Wil Naleratcsaletite aes Saticnte lea seeawi tuned tas eans echoes 7 5 Option lt 1 gt
63. tart the capture file now Then press any key to start the transfer The cache file remains in EEPROM until overwritten during the next deployment Start time 01 16 2007 15 31 34 Volume pumped 25 ml Elapsed time 21 sec Volume reached Lowest battery 31 5 Vv Start temp 20 6 C Port used 01 Stop time 01 16 2007 15 32 00 Start time 01 16 2007 18 14 18 Volume pumped 25 ml Elapsed time 20 sec volume reached Lowest battery 31 5 Vv Start temp 23 0 C Port used 48 Stop time 01 16 2007 18 14 47 End of EEPROM data backup cache Terminate file logging operation now and press any key to continue Figure 7 28 EEPROM Data Backup Cache Make backup copies of the capture file EgVicLA 7 19 lt 8 gt Contacting McLane serial number This option displays McLane contact information and includes the firmware version and McLane Research Laboratories Inc Falmouth Technology Park 121 Bernard E Saint Jean Drive East Falmouth MA 02536 USA Tel 508 495 4000 Fax 508 495 3333 Email mclane mclanelabs com www http www mclanelabs com Software version RAS100_5 c Compi led Jan 5 2007 09 56 29 Electronics S N ML11111 01 7 20 Figure 7 29 McLane Contact Information EaVicLANE Chapter 8 Data Offload and Processing After the RAS 100 is recovered and rinsed and the sample bags are removed for storage and analysis complete these steps to run the Offload Data opti
64. ternal thermistor can be installed to record temperature see Appendix B Optional External Temperature Sensor The Stepper circuit drives the stepper motor of the multi port valve under the direction of the firmware The Stepper circuit also monitors an optical switch in the motor housing to determine rotor position and precisely align the valve to each port The bottom circuit board is a TattleTale 8 TT8 version 2 micro controller manufactured by Onset Computer Corporation www onsetcomp com The TT8 is a single board computer with large and varied I O capacity that controls hardware operation including serial communication ports digital analog and timing interfaces a PIC 16C64 microcontroller serving as a programmable clock non volatile flash memory and RAM storage of the deployment data file with non volatile EEPROM backup Back up Battery Pump Communications Valve Main Battery Figure 3 2 Three Boards of Electronics Stack 3 2 cLA NE RESEARCH LABORATORIES INC Battery Connection Connecting and disconnecting the main battery is the only way to switch the RAS 100 on and off The main and back up battery connectors are keyed to prevent misconnection Disconnect both the main battery and the backup battery before performing a firmware reset To connect the main battery complete the following steps 1 Boot the operator PC and start the communications software 2 Open the controller housing and
65. the face o ring remains seated in its groove and that the radial o rings enter the housing uniformly without pinching Pump Gears Periodically examine the graphite pump gears and replace them if there are any visible signs of wear or cracks To inspect the gears complete the following steps 1 Remove the pump from the RAS 100 2 Remove the four screws that hold the pump head to the pump housing 3 Lift the pump head up off the pump housing the gears are mounted on the bottom of the pump head Never remove the metal magnet cavity from the pump motor housing the area under the metal cavity is filled with oil Battery Maintenance The capacity of a standard RAS 100 new alkaline battery pack A21 1000 is 10 000 mAhr The 31 5 volt battery will last up to 18 months This is a mildly conservative figure assuming a low temperature environment and standard current drain It is strongly recommended to use a fresh battery pack for each deployment Battery Replacement Running Option lt 2 gt Diagnostics from the RAS 100 Main Menu displays battery voltage A warning message will display after Diagnostics is run and immediately before a deployment commences if the battery voltage is below 28 volts The battery should be replaced when the voltage falls below 28 volts if a full deployment is planned ne cLANE RESEARCH LABORATORIES INC Before replacing the battery offload all data from the RAS 100 Unless a working 9V
66. u eee An ete 2 2 Top and Bottom End Capsycs srccsalestesanasaayasaisbannetadbsiacesdan a RTS EATE ERA ARES 2 3 End Cap Bulkhead Connectors vse Gsseistecsescassbesecevek shanties ianssasstersacieek aad ats 2 3 End Cap Bolts Plastic Inserts and Screw Holes cccccccsseceseceeeeeeeeeeseeeeteeeeeenees 2 5 Sample Chambers iones renei avin py E fe aa asian O E E E aed ohana 2 6 Pumping and Flushing ystems i 7 ca as cesuos cavccerncde cess adecanetstadacaaecvee tae tesereutcs mae 2 6 Gear PUMP irrena r E E a EE uae O N O wiclatul sels 2 6 Water Flush Assembly mss iirenicnnanrea ia i a a eee oa 2 8 Seral N MDET ia e ria RT naa R r a a a a a A 2 10 Chapter 3 Electronic Description ccscssscccsssccssssssssssccsssscesssscsssscsssscssssscsssosssssees 3 1 Controller Electronics Stack a raaecchsccuaecdte tihessuased tase tentoa vues Gometetatesausdeeade waesaeheechiees 3 1 Battery Connections snina Gare Stein ais sues Bachan hd ahing te held delet toes a et os 3 3 Chapter 4 Maintenance and Storage cccssccssssccssssccssssscssssscssssssssssssssssssessssssesseees 4 1 Cleaning and Inspecting the Controller Housing cccccccecsseeeteceeeeeeeeeeeeesseeneenees 4 Controller HOUSIt 8 istics ienai tiie Aiave Dosa teats E E R A des 4 C RAMS st ac0snisscdssanscaahaas faataasaseasadbansasetaus bans taaaabiachess A ESE 4 1 S100 0 Gite e EE E E E A E E E 4 2 Battery Maintenant eane ena Ta os Oceans e a E a a a 4 2 Battery R
67. window is written to the file 6 Toggle file logging by selecting File and Capture again 7 To exit Crosscut for Win select File from the menu bar and Exit from the submenu The format of the captured data file is ASCII text therefore use the extension TXT for the file name ASCII text files can be read by word processors and text editors ace KAMSLANE Crosscut for Win requires a name for the log file the first time the capture utility is called during a Crosscut for Win session That name then becomes the default for the remainder of the session The file can be renamed after capture on the hard disk of the PC Connecting the RAS 100 to a PC Connecting a DB 9 or DB 25 connector to a PC serial port by rocking the connector back and forth can cause a Com Port Crash in the TT8 Ifa crash occurs remove the connector and then restore power to recover control of the RAS 100 The crash is caused by signals or apparent signals on the receive pin of the communications port if they arrive when the TT8 is in low power sleep and if the ground connection between the TT8 and the PC is intermittent To connect and disconnect the communications cable complete the following steps 1 Always boot the PC and start Crosscut before connecting the communications cable to the RAS 100 2 Connect the communications cable first to the PC and then to the RAS 100 3 Disconnect the communications cable first from the RAS 100
68. ws cscccssscsssscesseces A 1 MGI CT OSSC Uc E E A EA A AE A cue tec Senet A 1 Fir t Time Cros cut Useaneneionu endagi a e a E aca ERRE A 1 Editing the Registry to Enable Crosscut ss ssesessssessessrsseeesseseessessessessressessessees A 2 Capturing Data Files Using CrossCuitiss cau cats ceiesiscnsacduastodaacustacsetsoncdinea tas naeaty A 2 Using Crosscut fot Win rnei ae Gece earn ee ee Moe een A 3 First Time Crosscut for Win USC ise ccss cusses Sods chat eis cbenav contcapgncaeoneeeaseumeduanavecicas A 4 Capturing Data Files with Crosscut for W10 cccccescceeceeesceeseeeeeceeeeeeeeenseeeaeenes A 4 Connecting the RAS 100 to a PC sisiscsicectsdestoctanieestadee ued eatoassedeenesndiatiebtem dations A 5 Additional OCUMEeEN AON 29 essees Sees ceisbek dite i a AE A 5 Appendix B Optional External Temperature Sensor cssccssssccssssscssssccssccseeses B 1 Configuring the External Temperature Probe ecceeceeeceeesceesseeeeceteeeeeeeeseeeseeees B 2 EaVicLANE TOC 3 TOCA RMcLANE RAS 100 User Manual List of Figures Figure 1 1 RAS 100 Line Drawing Overall View cecceeseeseeseceecneeeeeeeeeeeeaees 1 3 Figure 1 2 RAS 100 Tool kat oe sci sacidascca ceayeestosecentcdas Saas ts sedave Avast uataseasedadnaveienieiees 1 6 Figure 2 1 RAS VOOR UN VIEW zrnin naa bes satan aaiaadiacasbata SOA ET R at 2 1 Figure 2 2 Controller Housings x7 sasaccs lt c3ecntad sadecdusctusstladedetiasutesaaragntec

(RAS) - 100 - McLane Research Laboratories, Inc.

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