ActiPix™ Cartridge Manual v1.1
Contents
1. ActiP x D100 Cartridge Manual Version 1 1 ActiP x D100 Cartridge Manual v 1 1 parallel array technology Paraytec Ltd 1a St George s Place Tadcaster Road York YO24 1GN Tel 44 0 1904 526270 Fax 44 0 1904 652101 Email support paraytec com Web www paraytec com www actipix com Part Number 110 2100 Revised 06 08 2009 Published by Paraytec Ltd York UK Copyright 2009 Paraytec Ltd All rights reserved Printed and produced in the UK a Table of Contents UNO S i 8 1 1 Symbol COHFVODILIOI ceu nlt et pateat ek talia 8 12 Saf ly PrecautloriS sconti ode soc estu count ax salan merlin a iadesac snm cKM ilimiz 8 1 3 Impaired Safety Protection nennen nennen 9 1 4 CGE suc 10 1 5 SolventsandEleciricity nnns 11 15 Recycling INIONMANON ssidtasernnaseiesrandutewsuesienasnavelweaeatndeniaadsterseciidusncatsiusncatiliderteaeadie 11 2008 1 OCU COIN PER RR O TT 12 Pila SEERE 12 3 0 ChoosingaCartridge eee 13 3 1 Basic hp A 13 3 2 Cartridge Types and Descriptions ii 14 FP 21 il PTs 21 4 2 MakingaWindowinaCapllay AAMMMAMAMAMAe e e e gt memAmmm m m gt m m m m m nnn 22 4 3 Installation of Capillary in CE or LC Cartridge 24 4 4 n2. Creating a detection WINdOW nnne nnns 24 Figure 14 Holder and receiver nna nnn nnn 27 List of Tables Table 1 Overview of types of cartridges and part numbers 14 Table 2 Fused silica capillaries 10M lengths gt 18 The ActiPix D1 Read this first 00 is a high performance UV area imaging system with high precision electronic and sensitive optical components Please read Chapter 1 of this User Manual and Quick Start guide in the Software Manual before attempting any assembly or operation Please also familiarise yourself with all hazards which are highlighted in this manual before commencing operation The following symbols represent key areas where you will need to pay extra attention CAUTION Is used to indicate correct operating or maintenance procedures that prevent damage to or destruction of the equipment or other property A WARNING Is used to indicate a potential danger that requires E correct procedures or practices to prevent personal injury ActiPix D100 is a registered trademark of Paraytec Ltd Installation Date Warranty End Date Service Engineer Control Box Serial Number Sensor Serial Number SECT ON 1 0 SAFETY This section must be carefully read before unpacking installing using maintaining or repairing the instrument and or its accessories 1 1 Symbol Conventi
3. Creating a detection window After the capillary has been charred apply a small enough amount of alcohol to the lab wipe such that it is wet but not dripping Now place the capillary on a flat surface and with the wet wipe wipe the charred area with a smooth firm swipe away from the holding position ONLY The capillary is quite strong still but a motion of wiping back and forth will certainly cause the window to break As long as the surface is flat there should be little danger of breaking the capillary from pressing down Rotate the capillary if required and wipe to remove char from the back After the entire charred polymer has been removed inspect the new window with the eye glass from the tool kit If any small pieces remain they must be removed with further wiping action This can also be done on the white paper background NEVER apply the flame a second time to the capillary as this will cause permanent damage to the new window Once the polymer coating has been removed the window is extremely fragile and must be kept flat at all times including while installing in the cartridge ANY TORQUE APPLIED TO THE CAPILLARY WILL CAUSE IT TO BREAK AT THIS POINT 4 3 Installation of Capillary in CE or LC Cartridge The well established separation techniques of capillary electrophoresis CE and nano liquid chromatography nanoLC normally use fused silica capillaries Use of the ActiPix D100 detector with CE or nanoLC typi
4. ferrules that is not well understood is that they are made oversized and have to be shrunk down through a wedging action to activate them This activation process might actually take up to three lots of tightening to wedge the ferrule enough that it constricts around the tubing Once a ferrule has been wedged it can not return to any other position Ferrules that have been over tightened will appear rounded or squished and are probably unusable SECTION 6 0 Large Area Imager Using the quartz plate The large area imager allows visualization of almost any substance and can be used complementarily with an inverted microscope To remove the quartz plate simply remove the bottom plate of the cartridge and gently push the quartz plate out of the cartridge To install the quartz plate reverse the steps above The quartz plates have an internal lip that is approximately 200 um high but can be ordered in larger heights if more volume is required Because quartz is very inert the quartz slide can be sterilized or cleaned with any reagents The inner surface can be coated with a variety of materials for cell adhesion or particle interaction The cartridge comes with two 45 degree entrance holes for syringe needles for injecting reagents or solutions E 1 31 SECTION 7 0 Cleaning Cartridges All cartridges are made from a black polymer composite A lint free slightly damp wipe will remove most subst
5. 01NANO200M for10 32 male fitting Figure 3 NanoLC cartridge female NanoLC cartridge Description C101NANO200M ActiPix nanoLC cartridge for for10 32 male fitting Reusable Comes pre loaded with 200 um OD 75 um ID capillary for UV with nanoLC systems C101NANO200M for10 32 male fitting Figure 4 NanoLC cartridge male TT z A m CE cartridge for use with 200 um OD Description capillaries ActiPix cartridge for use with 200 pm OD or C102CE200 360 OD capillaries C102CE360 Reusable For use with 200 um OD 75 um ID capillary or 360 um OD 100 um ID capillary or UV detection with CE or capLC systems Contains spacing combs for even mounting Figure 5 CE cartridge Part Number C106MP200 or CE106MP360 Description ActiPix 6 capillary multiplex cartridge for use with 200 um OD or 360 OD capillaries Reusable For use with 200 um OD 75 um ID capillary or 360 um OD 100 um ID capillary or UV detection with CE or capLC systems Contains spacing combs for even mounting Figure 6 6 capillary multiplexed cartridge ET v r Fused silica capillaries 10m lengths FSA360075C ransparent fused silica capillary 363um OD 75um ID Table 2 Fused silica capillaries 10m lengths nI Cartridges for area imaging applications Part Number CAMEMST 1 Description ActiPix cartridge for monitoring diffusion processes static flow ActiPix cartridge for imaging diffusion p
6. 9 336 EEC Electromagnetic Compatibility Directive EMC 2006 95 EC Electrical Equipment designed for use within certain voltage limits Low Voltage Directive Such marking indicates that the system meets or exceeds the technical standards listed in the Declaration of Conformance Declaration of Conformity according to ISO IEC Guide 22 and EN 45014 Manufacturer Paraytec Ltd Address 1a St George s Place Tadcaster Road York YO24 1GN The above manufacturer declares that the ActiPix D100 UV detector conforms to the following standards Safety EC61010 1 2001 UL 61010 1 2004 EMC EN61326 1 1997 A1 1998 A2 2001 A3 2003 Electromagnetic compatibility EMC equipment for measurement control and laboratory use FCC ANSI C63 4 2003 and CFR47 Part 15 2003 Code of Federal Regulations Notices 1 Use only Paraytec Ltd approved parts 2 Use shielded cables supplied by Paraytec Ltd when connecting the ActiPix D100 UV detector to other equipment Compliance with the above two notices is necessary to ensure that the appropriate radio frequency emissions will be maintained within the limits of the specifications referred to in this declaration If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be rendered ineffective 1 5 Solvents and Electricity No solvents are required inside the control box Solvents should NEVER be placed applied or spilled inside th
7. ances Since most organic solvents will damage at least one of the plastics in the composite only water should be used for cleaning
8. cally requires insertion of a detection capillary in the flow line that must have a window burned in al 0 0 0u 0o0opp0 J Helpful HINT read how to properly tension cap llary in section 4 1 The location of the window from either end of the capillary should first be measured and the polymer coating removed as directed in section 2 4 above The window size should be between 10 and 25 mm see section 4 2 It is essential that the window be positioned directly in the centre of the viewing area For further information on installing the nanoLC capillary refer to Tech Note 3 nstalling a window in nanoLC 4 4 Inserting a Dissolution Cassette Dissolution cartridges do not use capillaries but are connected with PEEK tubing of various ID dimensions The quartz cells are rectangular tubes that are inserted by pressing in from either side of the cartridge The PEEK tubing is connected to both the tablet or membrane holder and the receiver cassette These in turn insert into the quartz cell located inside the cartridge Once the holder and receiver are inserted they should be secured by tightening the two M3 screws refer to figure below gently until a seal is made Label the screws and put textboxes in Fig below which also needs a label At the base of the holder and receiver are O rings that form the leak proof seal on the quartz cell If the holder or receiver are not tightened enough a leak will occur S
9. e ID is also extremely important in order to minimize turbulent zones at discontinuities in the flow profiles 5 2 Basic Types of Connectors Connectors come in two general sizes based on the thread size All fittings use American thread gauges These are 6 32 or 6 UNC and 10 32 or 10 UNC The 6 32 fitting connectors typically but not always require a sleeve that has an outer dimension of 1 32 OD 0 75 mm whereas the 10 32 always use a sleeve that measures 1 16 OD Sleeves come in the two outer sizes listed above but have a variety of inner diameters based on capillary or tubing used The optimum sleeve material is Teflon or PTFE All connectors use a ferrule locking system It is important that this system be fully understood before assembling 5 3 Sources for Connectors www upchurch com www vici com 5 4 Troubleshooting Connections Most tubing connections work using the principle of ferrules A ferrule is either a replaceable wedge or a one piece wedge that when connected into a female receiver causes the wedge tip to drive inward in this case into the sleeve or tubing and create a seal Almost every ferrule will reseal However an over tightened ferrule will become too small and leak it may also pinch too far in on the tubing and pinch it closed Some ferrules are reusable from tube to tube but only a handful of times Whenever possible a ferrule that has been set should be left sealed A fact about
10. e control box The only user serviceable part is the filter holder which can be removed for cleaning For optimum performance and care of your instrument ensure that the D100 and corresponding PC have a stable clean and uninterrupted source of mains power It is highly recommended to use a surge protection device however a primary level of protection is provided by specialised components inside the control box 1 6 Recycling Information Paraytec recommends that customers dispose of their used cartridges capillaries connectors cables sensor head assemblies and control boxes in an environmentally sound manner Potential methods include reuse of parts or whole products and recycling of products components and or materials 1 6 1 Waste Electrical and Electronic Equipment WEEE Directive In the European Union this label indicates that this product should not be disposed of with household waste It should be deposited at an appropriate facility to enable recovery and recycling For information on how to recycle this product responsibly in your country please contact Paraytec at info paraytec com SECTION 2 0 Introduction 2 1 Overview The ActiPix D100 opens up many new exciting possibilities for researchers This revolutionary instrument is a smart sensitive imaging system that has been designed for use in a variety of applications in research development and quality control In order for the D100 to perform al
11. ends on the application Typical applications are in measurement of time dependent absorbance of analyte peaks after or during a liquid phase separation e g liquid chromatography LC or capillary electrophoresis CE All cartridges for imaging use a quartz cell or plate Such cartridges are not designed for use with a capillary and typically have a sample imaging area 5 or 10 times larger than that used with capillaries Imaging applications include monitoring bulk solution or surface processes and reactions Descriptions of all cartridges and the applications for which they have been designed are given in the following section The applications mentioned in section 3 2 are far from exhaustive In addition to the cartridges shown here Paraytec has the ability to custom design cartridges tailored to your needs For more advice on possible uses of the ActiPix system contact the sales team at sales paraytec com zz r vac gt amp WY D amp CX O Imaging 3 2 Cartridge Types and Descriptions 3 2 1 Table of Cartridge Types and Part Numbers a Type of Cartridge Part Number Description size capillaries Multiple 200 um 1or2 Multi application applications using cartridge C102MA200 single cartridge applications using C102MA360 single cartridge C102SIZE200 for nanoLC nanoLC cartridge C101NANO200F separations for nanoLC C101NANO200M separations CE cartridge C102CE200 separations Lo
12. gile components such as the fused silica capillary or the protective cover slip Operating manual Figure 8 only handle using protective gloves and eyewear If the protective cover slip over the sensor is damaged do not operate the equipment Unplug the sensor cable to avoid any possible damage to the sensitive electronics inside and notify your supplier immediately Solvents used in applications can be corrosive poisonous or flammable and suitable care should be taken in handling them In the UK it is a requirement to comply with the Control of Substances Hazardous to Health Act 1989 COSHH During operation warm air is vented from the rear side of the ActiPix control box Operating manual Fig 5 All cables and pipework must be clear of this airstream and items adversely affected by heat should not be placed in close proximity When hazardous chemicals are being analysed care must be taken to provide the necessary level of ventilation Do not stack any items on any of the components of the ActiPix unit Impaired Safety Protection Whenever it is likely that safety protection has been impaired the instrument and or accessory must be made inoperative and secured against operation The matter should then be referred to the appropriate servicing authority for remedial action 1 4 CE Notice Marking with the symbol CE indicates compliance of the ActiPix D100 UV detector to the following European Directives 8
13. hould this happen simply tighten further With time the O rings will fail naturally due to material degradation and will have to be replaced The holder and receiver are interchangeable to accommodate ease of assembly in the lab This has no effect on data or performance Flow Originating from the left side of the cartridge is seen on the left side of the image 3 r Quartz Cell Dissolutlon cassette Figure 14 Holder and receiver The dissolution holders have been engineered to shape the bulk flow to make the flow parallel to the exposed surface of the tablet section The flow profile will work for any tablet section provided it is mounted with its exposed surface 0 1 5 mm above the top surface of the holder Above 1 5 mm the flow profile will likely cause turbulence and disruption of the flow profile A gap exists between the holder and receiver so that there is no pressure against them when the seal is made against the guariz I SECTION 5 0 Tubing and Connections 5 1 Types of Tubing There are two types or tubing available to make connections to a cartridge polymer or fused silica Polymers come in many varieties The application will determine which is the best material For use with connectors a critical variable is the outer diameter OD Since the detection zone is typically fused silica for most cartridges all tubing will have to be reduced down to either 200 or 360 um OD Matching of th
14. l of its unique capabilities a cartridge is required for proper use The cartridge serves many functions for example i excluding ambient light from the imaging area and ii accurate positioning of the capillaries or cells with good repeatability No proper imaging can be accomplished without the use of the specially designed cartridges Cartridges have been designed to make them easy to assemble and practical to use Most of the cartridges can be used fully assembled in other instruments such as inverted microscopes Careful choice of the cartridge for the desired application is required for optimal performance of the system Whilst all have been designed to enhance performance in one particular application many cartridges offer flexibility in use To discuss the viability of using cartridges for novel applications do not hesitate to contact Paraytec staff sales paraytec com zz r SECTION 3 0 Choosing a Cartridge 3 1 Basic Information The ActiPix D100 has many versatile applications Because of this it is important to use the correct cartridge to get the best results for that application Choosing the correct type of cartridge depends on the application and the final data format absorbance plot or imaging movies For capillary absorbance cartridges there are several styles and each is available for capillaries with outer diameter OD either 200 or 360 um The style and OD of the capillary selected generally dep
15. on CAUTION Is used to indicate correct operating or maintenance procedures that prevent damage to or destruction of the equipment or other property WARNING Is used to indicate a potential danger that requires correct procedures or practices to prevent personal injury HINT Notice that user tips are shown in the dialog box These HINTS often contain important basic information and apply to all users 1 2 Safety Precautions 1 2 1 The safety statements in this manual comply with the requirements of the UK Health and Safety at Work Act of 1974 You must read this section to ensure the safe operation of your instrument 1 2 2 UV detection involves the use of high voltage power supplies and xenon flash lamps The instrument and accessories described in this manual must be used maintained and repaired by properly trained personnel only 1 2 3 The standard safety procedures in your laboratory should always be adhered to in addition to the advice given in this manual 1 2 4 1 2 5 1 2 6 1 2 7 1 2 8 1 2 9 1 2 10 1 3 Removal of any fixed covers may expose live electrical mains If this becomes necessary the instrument must be disconnected from the electrical mains and at least three minutes allowed for capacitors to discharge Replace fuses only with approved high breaking current type of the specified rating Operating manual Section 8 0 In the event of breakage of any fra
16. oped bioreactor aM um LM or 2 for pre and post EE Ei 1 RISUS zie dissolution Dissolution cartridge CDISSA 1 integrated holder DISSFT 1 MEMFT 1 VISQ 1 Spare holders and MEMST 1 1 visualization Dissolution extras VISSTQ 1 chambers 500 ul flat area e elr area imager EE 1 lizza plate le gt ED n a applications two Custom cartridge C100CUST iterations Table 1 Overview of types of cartridges and part numbers a 3 2 2 Quick Guide to Cartridge Types and Applications Multi application cartridge C102MA200 C102MA360 Description ActiPix multi application cartridge Reusable Can be used for applications requiring up to 2 capillaries e g sizing of pure species 2 capillary CE bioreactor studies Contains spacing combs for even mounting C102MA200 for use with 200 um OD capillaries C102MA360 for use with 360 um OD capillaries Figure 1 Multi application cartridge Cartridge for sizing applications C102SIZE200 Description ActiPix cartridge for sizing application Reusable Allows easy assembly of cartridge for sizing application Contains spacing combs for even mounting For use with 200 um OD capillaries Figure 2 Cartridge for sizing applications Tyv NanoLC cartridge Description C101NANO200F ActiPix nanoLC cartridge for 6 32 female fitting Reusable Comes pre loaded with 200 um OD 75 um ID capillary for UV with nanoLC systems C101NANO200F for 6 32 female fitting C1
17. ow the following tools are required e Lighter or open flame such as a candle e Disposable soft wiping cloth such as a lab wipe e An alcohol solvent e g ethanol methanol 2 propanol isopropanol or an alcohol gel e Ruler e Black marker re N SN AN e Flat smooth surface e White paper for visualization e Eyeglass After the overall length of capillary has been cut determine the location of the window This can be measured from either end but preferably from the flow inlet end If the length of capillary is important as is the case in CE and for sizing measure from the grub screw in the centre of the sensor head shown below This point represents the centre of the sensor with the actual sensor imaging area being 9 mm in width Figure 12 Close up view of Sensor Head The minimum window width is 10 mm and maximum 25 mm with the preferred width being 15 mm Using the ruler and the marker completely mark black a 15 mm section where the window is to go Next hold the capillary in one hand in front of you parallel to the body With the flame on the lowest setting move the flame towards and away from you not left to right along the capillary until the polymer coating is completely black and charred Do not allow the flame to remain in contact with the capillary after the polymer coating has completely charred Caution if the flame is applied too long the capillary will melt NN ME Figure 13
18. rient the cartridge as shown in Figure 11 below for assembly To do this removed the cartridge from the D100 and rotate 180 degrees toward you This will position the cartridge upside down for ease of access to the bottom plate screws and more importantly to maintain the capillaries respective to the sides they will have when replaced into the cartridge This becomes very important when making delicate fluid connections that need to flow in from the left or right as the case may be If the cartridge is not assembled in the proper manner when it is returned to the normal position in the D100 the fluid directions will be reversed and the inlet side of the capillary possibly too short uuricz x OOF pui OD vw Figure 11 Correct orientation for assembly of cartridges ETHER CCCCULCO VECCIOTCCO CE Once the capillary has been placed in the cartridge it is highly advised to apply tension to the capillary such that it remains in a straight line parallel to the face of the sensor If the capillary is allowed to curve several errors might occur in the absorbance 1 for upward curvature part of the capillary window will be further away from the cover plate surface and hence give a lower signal 2 the capillary might move with vibrations or just with time thereby falling out of the pixel map assigned in the cartridge file with the result being a dramatic rise or fall in signal 3 the capillary might break resulting in a lost experimen
19. rocesses with static flow Comes complete with MEMST1 1 Inline membrane diffusion holder static version shown VISSTQ 1 replacement quartz visualisation chamber static spares can be ordered separately Figure 7 ActiPix cartridge for monitoring diffusion processes static flow Part Number CAVIVO 1 Description ActiPix large area imaging cartridge ActiPix cartridge for imaging processes ona large viewing area Figure 8 Large area imaging cartridge a Part Number C100CUST 1 Description Custom cartridge holders for specialist applications 2 iterations Contact Sales Paraytec offer a custom cartridge design service for specialist applications not listed Please contact sales paraytec com for more details Figure 9 Custom cartridge holders ert ke fe Fibre optic gap Height from capillary to top Pd 56 h Figure 10 Diagram of cartridges with dimensions SECT ON 4 0 Cartridge Preparation 4 1 Basic Information Proper assembly of a cartridge is vital for connecting to other equipment and to avoid breaking capillaries NOTE Capillary cartridges are designed for two sizes of capillary 360 um or 200 um OD Forcing 360 um capillaries into the 200 um cartridge combs can cause breakages and fluid leakages that may damage the ActiPix sensor head Inserting 200 um into 360 um will increase the probability of capillary movement and lead to poor data To begin ALWAYS o
20. sertingaDissolutiinCassette 25 5 0 TubingandConnecilons lee 28 Sd lt lt ep RET 28 5 2 Basic Types of Connectors ii 28 ES Ol COMING CONG TELE 28 5 4 TroubleshootingConnections gt gt nnns 29 6 0 Large Area Imager Using the quartz plate 30 7 0 eng 31 List of Figures Figuree1 Multi applicationcartridge m m m 15 Figure 2 Cartridge for sizing applications enn 15 Figure3 NanolCcartrildge female mmmmmA A m m m nnns 16 Figure 4 NanoLC cartridge male sess nennen nnns 16 ael gow cyan eg e ETE m m mmm 17 Figure 6 6 capillary multiplexed cartridge m 17 Figure 7 ActiPix cartridge for monitoring diffusion processes static floW 19 Figure 8 Large area imaging cartridge nennen 19 Figure 9 Custom cartridge holders nennen nnne nnn nnns 20 Figure 10 Diagram of cartridges with dimensions esses 20 Figure 11 Correct orientation for assembly of CartridgeS nn 21 Figure 12 Close up view of Sensor Head ii 23 Figure 13
21. t or damage to the cartridge for information on leaks leading to presence of liquid inside the sensor please refer to the User Manual To properly tighten the capillary inside the cartridge begin by placing the capillary in the required location then place the bottom plate in place with the four 4 screws ready to be tightened begin to tighten the right side of the cartridge only now apply a straight constant outward pulling tension to the capillary on the left side once tension is applied tighten the screws on the left side In summary the right side of the For confirmation of a capillary is held in place with the clamping action of the right screws correctly tensioned capillary in On line mode open an then tension is applied to straighten the capillary while simultaneously imaging method to view the tightening the left side screws capillaries If they appear curved repeat the process a 4 2 Making a Window in a Capillary All capillaries come with a protective polymer coating on the outside Whilst this coating is in place the capillaries enjoy enormous flexibility and resilience However the polyimide coating brown or TSP type does not transmit light and must be removed prior to installation PTFE coated or TSU type capillaries do not require their coatings to be removed but suffer from loss of signal and increase in stray light through scattering by the polymer To remove the outer polymer coating to make a viewing wind
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