TLN-100 Rotor - American Instrument Exchange, Inc.
Contents
1. 8 31 2 mL Approximate acceleration time to maximum speed fully loaded 3 1 2 min Approximate deceleration time from maximum speed fully loaded 2 1 2 min Weight of fully loaded rotor 0 9 kg 2 0 Ib Rotor material bes 4d eee cede titanium Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed ro to the standard acceleration of gravity g according to the following formula 8 where is the radius in millimeters is the angular velocity in radians per second 2 z RPM 60 and g is the standard acceleration of gravity 9807 mm s2 After substitution RPM 1000 RCF 1 12r TLN 100 Rotor DESCRIPTION This Beckman Coulter rotor has been manufactured in a registered ISO 9001 or 9002 facility for use with the appropriately classified Beckman Coulter ultracentrifuge The TLN 100 rated for 100 000 rpm is a near vertical tube rotor with a tube angle of 9 degrees from the axis of rotation The rotor can centrifuge up to eight tubes and is used in Beckman Coulter Optima MAX MAX XP MAX E TL TLX and the TL 100 tabletop ultracentrifuges The slight angle of this rotor significantly reduces run times from a more conventional fixed angle rotor with a tube angle of 20 to 45 degrees whil2. 1 9 Equation 8 1 8 1 7 ay 2 c 2 3 0 Q 1 6 o 2 c I 1 5 TLN 100 ROTOR 20 4 C 1 4 1 3 0 10 20 30 40 50 60 70 80 90 100 Rotor Speed rpm Figure 2 Precipitation Curves for the TLN 100 Rotor Using combinations of rotor speeds and homogeneous CsCl solution densities that intersect on or below these curves ensures that CsCl will not precipitate during centrifugation The dashed line is a representation of equation 6 and is shown here to illustrate the inability of that equation to guard against CsCl precipitation Density g mL 1 9 1 8 1 7 1 6 1 5 1 4 1 3 from Figure 2 results in gradients presented here The homogeneous CsCl solution density DIT 1 9 9 000 qm e 1 1897 x Q9 4098 WV m S rom 9f 000 60 eS Ne e 9 SS KS qo 2 eS 4099 SAAS Lot AQ e RM Q 1 om eS be TLN 100 ROTOR CE 206 4 C O X KY oS 23 1 25 2 27 4 29 5 31 7 33 8 35 9 38 1 40 2 Distance from Axis of Rotation mm Figure 3 CsCl Gradients at Eq
3. 50 000 0 0 20 000 40 000 60 000 80 000 100 000 Speed rpm 11 TLN 100 Rotor Speeds must be reduced under the following circumstances 1 If nonprecipitating solutions more dense than 1 7 g mL are centrifuged the maximum allowable run speed must be reduced according to the following equation reduced maximum speed 100 000 rpm 11 7 g mL 6 where p is the density of the tube contents This speed reduction will protect the rotor from excessive stresses due to the added tube load 2 Further speed limits must be imposed when CsCl or other self forming gradient salts are centrifuged as equation 6 does not predict concentration limits speeds that are required to avoid precipitation of salt crystals Precipitation during centrifugation would alter the density distribution of CsCl and this would change the position of the sample bands Figures 2 and 3 together with the description and examples below show how to reduce run speeds when using CsCl gradients SELECTING CsCl GRADIENTS Precipitation during centrifugation would alter density distribution and this would change the position of the sample bands Curves in Figures 2 and 3 are provided up to the maximum rated speed of the rotor gt NOTE The curves in Figures 2 and 3 are for solutions of CsCl salt dissolved in distilled water only If other salts are present in significant concentra tions the overall CsCl concen
4. 2 450 000 x g 14 Includes disposable plastic plugs Coo gt Temperature Limits Plastic tubes have been centrifuge tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated run conditions e If plastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation OptiSeal Tubes OptiSeal tubes come with plastic plugs and be quickly and easily prepared for use With the tube spacer in place the g force during centrifugation ensures a tight reliable seal that protects your samples TLN 100 Rotor Place the tubes in the rack and fill each tube to the base of the a Plug stem leaving no fluid in the stem Overfilling the tube can cause PR Assembbly spillage when the plug is inserted or can compromise seal integrity Spacer However too much air can cause excessive tube deformation disrupting gradients and sample bands OptiSeal Tube Refer to Using OptiSeal Tubes publication IN 189 included in each box of tubes for detailed information on the use and care of OptiSeal tubes Quick Seal Tubes Quick Seal tubes must be sealed prior to centrifugation These tubes Black ad are heat sealed and do not need caps however spacers are required ner a on top of the tubes when they are loaded into the rotor i Fill Quick Seal tubes leaving a small bubble of a
5. 49 21 51 33 33 e mail bioresearch de beckman com C COULTER Beckman Coulter Inc Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 Fax 852 2873 4511 INDIA Beckman Coulter India Pvt Ltd Solitaire Corporate Park 3rd Floor Bldg 11 Andheri Ghatkopar Link Road Chakala Andheri West Mumbai India 400 098 Telephone 91 22 3080 5101 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 39 02 953921 Fax 39 02 95392264 JAPAN Beckman Coulter K K TOC Ariake West Tower 2 5 7 Ariake Koto ku Tokyo 135 0063 Japan Telephone 81 3 5530 8500 Fax 81 3 5404 8436 MEXICO Beckman Coulter de Mexico S A de C V Avenida Popocat petl 396 Colonia Gral Pedro Maria Anaya Codigo Postal 03340 Mexico D F Mexico Telephone 001 52 55 9183 2800 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 31 297 230630 Fax 31 297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Telephone 65 6339 3633 Fax 65 6336 6303 SOUTH AFRICA SUB SAHARAN AFRICA Beckman Coulter Stand 1A Primegro Park Tonetti Street 1685 Halfway House Johannesbu
6. CTIVE NON PATHOGENIC NON TOXIC AND OTHERWISE SAFE TO SHIP AND HANDLE IS REQUIRED Beckman Coulter Worldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 1 905 819 1234 Fax 1 905 819 1485 CZECH REPUBLIC Beckman Coulter Prague Radiova 1 102 27 Prague 10 Czech Republice Telephone 420 267 00 85 13 Fax 420 267 00 83 23 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 365 3707 Fax 41 22 365 0700 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 33 1 49 90 90 00 Fax 33 1 49 90 90 10 e mail bioresearch france beckman com GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 51 33 35 Fax
7. Printed on recycled paper
8. REMOVAL AND SAMPLE RECOVERY Plunger Released N CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories 1 To release the plunger at the end of the run gently press it down until you feel it click When you remove your finger the plunger will pop up to its released position 2 Remove the rotor from the ultracentrifuge and place it in the rotor vise 3 Remove the rotor plugs taking care to apply downward pressure on the plug adapter to avoid stripping the plugs 4 Use a tube removal tool to remove the spacers and tubes TLN 100 Rotor TUBES AND ACCESSORIES The TLN 100 rotor uses tubes and accessories listed in Table 1 Be sure to use only those items listed and to observe the maximum speed limits shown Refer to Appendix A in Rotors and Tubes for information on the chemical resistances of tube and accessory materials Table 1 Available Tubes for the TLN 100 Rotor Use only the items listed here and observe maximum fill volumes and speed shown Tube Required Accessory Max Speed Dimensions Part Part RCF and Volume Description Number Description Number k Factor 13 x 38 mm Quick Seal 358980 black aluminum 342883 100 000 rpm 3 9 mL polyallomer pkg 50 spacer pkg 8 450 000 x g 14 13 x 33 mm OptiSeal 361627 gold aluminum 362198 100 000 rpm 3 3 mL pkg 56 spacer pkg
9. TL TB 013E 5 September 2007 BECKMAN COULTER TLN 100 Rotor Used In Beckman Coulter Optima MAX XP MAX MAX E TL TLX and TL 100 Tabletop Ultracentrifuges 2007 Beckman Coulter Inc Produced in the U S A TLN 100 Rotor SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted When you see the symbol on other pages throughout this publication pay special attention to the specific safety informa tion presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter ultracentrifuge rotor system Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter ultracentrifuge that has been modified without Beckman Coulter s approval A E ps pepe gt Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emph
10. Using the plug adapter 976959 and torque wrench 858121 torque each rotor plug to 13 6 Nem 120 in Ib 7o avoid stripping the plugs apply downward pressure to the plug adapter while tightening the plugs Do not overtighten plugs Use an absorbent towel to wipe off condensation from the rotor then carefully place the rotor on the drive hub Lock the rotor in place by gently pressing the plunger down until you feel it click When you remove your finger the plunger will remain flush with the rotor body if it is properly engaged If the plunger pops up repeat the procedure The Optima MAX MAX XP and MAX E ultracentrifuges automatically secure the rotor to the drive shaft without the need for engaging the plunger TLN 100 Rotor A CAUTION In all ultracentrifuge models except the Optima MAX MAX XP and MAX E it is very important to lock the rotor in place before beginning the run to ensure that the rotor remains seated during centrifugation Failure to lock the rotor in place before beginning the run may result in damage to both rotor and instrument 3 Refer to the instrument instruction manual for ultracentrifuge operation 4 For additional operating information see the following RUN TIMES page 9 for using factors to adjust run durations RUN SPEEDS page 10 for information about speed limitations SELECTING CsCl GRADIENTS page 12 for methods to avoid CsCl precipitation during centrifugation
11. and Replacement Policies 1 Ifa Beckman Coulter rotor is determined by Beckman Coulter to be defective Beckman Coulter will repair or replace it subject to the Warranty Conditions A replacement rotor will be warranted for the time remaining on the original rotor s warranty 2 Ifa Beckman Coulter centrifuge is damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge 1 all centrifuge parts required for repair except the drive unit which will be replaced at the then current price less a credit determined by the total number of revolutions or years completed provided that such a unit was manufactured or rebuilt by Beckman Coulter and ii if the centrifuge is cur rently covered by a Beckman Coulter warranty or Full Service Agreement all labor necessary for repair of the centrifuge 3 If a Beckman Coulter rotor covered by this warranty is dam aged due to a malfunction of a Beckman Coulter ultracentrifuge covered by an Ultracentrifuge System Service Agreement Beckman Coulter will repair or replace the rotor free of charge 4 If a Beckman Coulter rotor covered by this warranty is damaged due to a failure of a Beckman Coulter tube bottle tube cap spacer or adapter covered under the Conditions of this Warranty Beckman Coulter will repair or replace the rotor and repair the instrument as per the conditions in policy point 2 above and the replacement policy 5 Damage to a Beck
12. asize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol contain ment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the ultracentrifuge Although rotor components and accessories made by other manufacturers may fit in the TLN 100 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the TLN 100 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density Make sure that cavities in use have the proper spacers and or floating spacers inserted bef
13. at a given radius in a rotor is a function of speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rota tional speed is adjusted so that identical samples are subjected to the same RCF in two different rotors the samples are subjected to the same force The RCF at a number of rotor speeds is provided in Table 2 TLN 100 Rotor Table 2 Relative Centrifugal Fields for the TLN 100 Rotor Entries in this table are calculated from the formula RCF 1 12r RPM 1000 and then rounded to three significant digits Relative Centrifugal Field x g Rotor Speed At Imax At ray At fmin k rpm 40 2 mm 31 6 mm 23 1 mm Factor 100 000 450 000 354 000 259 000 14 95 000 406 000 319 000 233 000 15 90 000 365 000 287 000 210 000 17 85 000 325 000 256 000 187 000 19 80 000 288 000 227 000 166 000 22 75 000 253 000 199 000 146 000 25 70 000 221 000 173 000 127 000 28 65 000 190 000 150 000 109 000 33 60 000 162 000 127 000 93 100 38 55 000 136 000 107 000 78 300 46 50 000 113 000 88 500 64 700 55 Calculated for all Beckman Coulter preparative rotors as a measure of the rotor s relative efficiency in pelleting sample in water at 20 C Relative Centrifugal Fields for the TLN 100 Rotor 500 000 450 000 400 000 350 000 300 000 RCF x g 250 000 200 000 150 000 100 000
14. ds lubri cated can result in damaged threads Replace rotor plugs as a set if they show signs of wear Inspect plug gaskets for wear or damage Replace as required To remove the old gasket squeeze it with your thumb and first finger until it comes away from the groove Slip it off and snap a new one in its place Be careful not to scratch the anodized surface of the plugs Refer to Appendix in Rotors and Tubes for the chemical resistances of rotor and accessory materials Your Beckman Coulter representa tive provides contact with the Field Rotor Inspection Program and the rotor repair center TLN 100 Rotor CLEANING Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Rotor Cleaning Kit 339558 Under normal use wash the rotor frequently at least weekly to prevent buildup of residues Wash the rotor plugs and spacers in a mild detergent such as Beckman Solution 555 339555 that won t damage the rotor The Rotor Cleaning Kit contains two plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water gt NOTE Do not wash rotor components in a dishwasher Do not soak in detergent solution for long periods such as overnight 2 Rinse the cleaned rotor and components with distilled water 3 Air dry the r
15. e allowing components that do not band under separation conditions to either pellet to the bottom or float to the top of the tube The rotor is made of titanium and is finished with black polyurethane paint A tube spacer and rotor plug hold each tube in the rotor and a plug gasket forms a closure around each plug Rotor plugs and spacers are anodized aluminum The ultracentrifuge identifies rotor speed during the run by means of a magnetic speed sensor in the instrument chamber and magnets on the bottom of the rotor This overspeed protection system ensures that the rotor does not exceed its maximum permitted speed See the Warranty at the back of this manual for warranty information PREPARATION AND USE Specific information about the TLN 100 rotor is given here Informa tion common to this and other rotors is contained in Rotors and Tubes for Tabletop Preparative Ultracentrifuges publication TLR IM which should be used together with this manual for complete rotor and accessory operation Publication TLR IM is included in the literature package shipped with the rotor PRERUN SAFETY CHECKS N ROTOR PREPARATION TLN 100 Rotor NOTE Although rotor components and accessories made by other manufacturers may fit in the TLN 100 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the rotor may void the rotor warranty and should be prohibited b
16. h pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office 19 TLN 100 Rotor 20 SUPPLY LIST I gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office Contact Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed on the back cover of this manual or see the Beckman Coulter Ultracentrifuge Rotors Tubes amp Acces sories catalog BR 8101 for detailed information on ordering parts and supplies For your convenience a partial list is given below REPLACEMENT ROTOR PARTS OTHER TLN 100 rotor 5 8 8 357614 Rotor plug does not include gasket 368545 Plug gasket OD 14 5 mm 0 57 1 342882 Cap and plunger 349339 ROUOP VISE siaiu eer er eee ERU LECHE md eder 347373 Tubes and see Table 1 Quick Sea
17. ir at the base of e the neck Do not leave a large air space too much air can cause excessive tube deformation e Refer to Rotors and Tubes for detailed information on the use and care of Quick Seal tubes RUN TIMES TIME ARMIN The k factor of the rotor is a measure of the rotor s pelleting efficiency Beckman Coulter has calculated the k factors for all of its p preparative rotors at maximum rated speed and using full tubes The k factor is calculated from the formula In fmax min 19 x 1 22 3600 where 015 the angular velocity of the rotor in radians per second 0 105 x rpm Fmax is the maximum radius and rq is the minimum radius TLN 100 Rotor 10 RUN SPEEDS SPEED RPM RCF After substitution 0 rpm Use the k factor in the following equation to estimate the run time t in hours required to pellet particles of known sedimentation coeffi cient s in Svedberg units 5 Run times can be estimated for centrifugation at less than maximum speed by adjusting the factor as follows 2 _ 100900 8 run speed 4 Run times can also be estimated from data established in prior experi ments if the k factor of the previous rotor is known For any two rotors a and b t k a a do E 5 th ky For more information on k factors see Use of k Factor for Estimating Run Times from Previously Established Run Conditions publication DS 719 The centrifugal force
18. l Cordless Tube Topper kit 60 gt 358312 Quick Seal Cordless Tube Topper kit 50 Hz Europe 358313 Quick Seal Cordless Tube Topper kit 50 Hz Great Britain 358314 Quick Seal Cordless Tube Topper kit 50 Hz Australia 358315 Quick Seal Cordless Tube Topper kit 50 Hz Canada 367803 Tube Topper rack sete eese ere me 355872 OptiSeal tube rack 361650 Torque wrench ere depu 858121 Plug adapter eR Rep OR le 976959 Tuberemoval tool ssc ee Oe OS rS eee Dens 361668 Curved hemostat 6 927208 Straight hemostat 961519 Fraction Recovery System 342025 Fraction Recovery System Adapter Kit for TL series tubes 347828 Beckman Coulter CentriTube 81 347960 CentriTube Slicer replacement blades pkg of 10 348299 CentriTube Slicer adapter for 13 mm 354526 Spinkote lubricant 2 2 306812 TLN 100 Rotor Silicone vacuum grease 1 OZ 335148 Rotor Cleaning Kit s else 339558 Rotor cleaning brush 347404 Beckman Solution 555 1 qt 339555 21 ULTRACENTRIFUGE WARRANTY Beckman Coulter u
19. ltracentrifuge Fixed Angle Vertical Tube Near Vertical Tube Swinging Bucket and Airfuge rotors are warranted against defects in materials or workmanship for the time periods indicated below subject to the Warranty Conditions stated below Preparative Ultracentrifuge Rotors 5 years No Proration Analytical Ultracentrifuge Rotors 5 years No Proration ML and TL Series Ultracentrifuge ROOTS oed tes bets is 5 years No Proration Airfuge Ultracentrifuge Rotors 1 year No Proration For Zonal Continuous Flow Component Test and Rock Core ultracentrifuge rotors see separate warranty Warranty Conditions as applicable 1 This warranty is valid for the time periods indicated above from the date of shipment to the original Buyer by Beckman Coulter or an authorized Beckman Coulter representative 2 This warranty extends only to the original Buyer and may not be assigned or extended to a third person without written consent of Beckman Coulter 3 This warranty covers the Beckman Coulter Centrifuge Systems only including but not limited to the centrifuge rotor and accessories and Beckman Coulter shall not be liable for damage to or loss of the user s sample non Beckman Coulter tubes adapters or other rotor contents 4 This warranty is void if the Beckman Coulter Centrifuge Sys tem is determined by Beckman Coulter to have been operated or maintained in a manner contrary to the instruction
20. man Coulter rotor or instrument due to the failure or malfunction of a non Beckman Coulter tube bottle tube cap spacer or adapter is not covered under this warranty although Beckman Coulter will assist in seeking compensation under the manufacturer s warranty Disclaimer IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFAC TURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT Factory Rotor Inspection Service Beckman Coulter Inc will provide free mechanical and metallurgical inspection in Palo Alto California USA of any Beckman Coulter rotor at the request of the user Shipping charges to Beckman Coulter are the responsibility of the user Rotors will be inspected in the user s laboratory if the centrifuge in which they are used is covered by an appropriate Beckman Coulter Service Agreement Contact your local Beckman Coulter office for details of service coverage or cost Before shipping contact the nearest Beckman Coulter Sales and Service office and request a Returned Goods Authorization RGA form and packaging instructions Please include the complete rotor assembly with buckets lid handle tube cavity caps etc A SIGNED STATEMENT THAT THE ROTOR AND ACCESSO RIES ARE NON RADIOA
21. ore installing the rotor lid If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS and derate the run speed as appropriate Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use TLN 100 Rotor TLN 100 ROTOR U S Pat Nos 4 102 490 4 290 550 Axis of Rotation Japanese Pat No 1 469 154 SPECIFICATIONS Maximum speed e eeete e RIA VERE UBER ERES EEG 100 000 rpm Density rating at maximum 1 7 g mL Relative Centrifugal Field at maximum speed IER YS 450 000 x g Atr 310gmm ese RR ERE ERES 354 000 x g At Eu 23 LIM eee oe ero Sue S e thats otis 259 000 x g k factor at maximum 14 Conditions requiring speed reductions see RUN SPEEDS Number of tube cavities 8 Available tubes a ee see Table 1 Nominal tube dimensions largest 13 x 38 mm Nominal tube 3 9 mL Nominal rotor
22. ot in use store the rotor in a dry environment not in the instrument with the plugs removed to allow air circulation so moisture will not collect in the tube cavities Flammability hazard Do not use in or near operating ultracentrifuges RETURNING A TLN 100 Rotor Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form may be obtained from your local Beckman Coulter sales office and should contain the following information serial number history of use approximate frequency of use reason for the return original purchase order number billing number and shipping number if possible name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated wit
23. otor upside down Do not use acetone to dry the rotor Gasket Missus Se Clean plug threads as necessary Use a brush and concentrated Solution 555 Rinse and dry thoroughly then lubricate lightly NS but evenly with Spinkote to coat all threads DECONTAMINATION If the rotor or other components are contaminated with radioactive toxic or pathogenic materials follow appropriate decontamination procedures as outlined by appropriate laboratory safety guidelines and or other regulations Consult Appendix A in Rotors and Tubes to select an agent that will not damage the rotor 17 TLN 100 Rotor 18 STERILIZATION AND DISINFECTION The rotor and all rotor components can be autoclaved at 121 C for up to an hour Remove the plugs and place the rotor and plugs in the autoclave upside down 121 C EPI Ethanol 70 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anod ized surfaces Use the minimum immersion time for each solution per laboratory standards mmm While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use STORAGE When it is n
24. quired run temperature 20 C The maximum allowable rotor speed is deter mined from the point where this curve intersects the homogeneous CsCl density 79 000 rpm 2 In Figure 3 sketch in a horizontal line corresponding to each particle s buoyant density and find the density gradient curve corresponding to the above speed 79 000 rpm 3 Mark the point in the figure where each particle density intersects the curve corresponding to the run speed 79 000 rpm and temperature 20 C 4 Particles will band at these marked points across the tube diameter lower axis of Figure 3 at equilibrium during centrifugation After centrifugation the bands will reorient upper axis In this example the particles will band along this curve about half of the way from the right edge of the figure Using the lower horizontal axis it can be estimated that these particles will about 1 mm apart at equilibrium during centrifugation Example B Knowing particle buoyant densities 1 59 and 1 61 g mL how do you get the best separation Assume 20 C operation 1 In Figure 3 sketch in a horizontal line corresponding to each particle s buoyant density 13 TLN 100 Rotor 14
25. rg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 e mail beckman intekom co za Internet www beckmancoulter com Telex 678413 2007 Beckman Coulter Inc SPAIN Beckman Coulter Espafia S A C Caleruega 81 28033 Madrid Spain Telephone 34 91 3836080 Fax 34 91 3836096 email bioresearch espana beckman com SWEDEN Beckman Coulter AB Archimedesvaegen 7 Box 111 56 SE 168 11 Bromma Sweden Telephone 46 8 564 85 900 Telefax 46 8 564 85 901 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 0800 850 810 Fax 41 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 44 01494 441181 Fax 44 01494 447558 e mail beckman_uk beckman com Additional addresses are available at www beckmancoulter com 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 Fax 1 800 643 4366 Bo
26. s in the operator s manual s for the Beckman Coulter Centrifuge System components in use This includes but is not limited to operator misuse abuse or negligence regarding indicated maintenance procedures centrifuge and rotor classification requirements proper speed reduction for the high density of certain fluids tubes and tube caps speed reduction for precipi tating gradient materials and speed reduction for high tempera ture operation 5 Rotor bucket sets purchased concurrently with or subsequent to the purchase of a Swinging Bucket Rotor are warranted only for a term co extensive with that of the rotor for which the bucket sets are purchased 6 This warranty does not cover the failure of a Beckman Coulter rotor in a centrifuge not of Beckman Coulter manufacture or if the rotor is used in a Beckman Coulter centrifuge that has been modified without the written permission of Beckman Coulter or is used with carriers buckets belts or other devices not of Beckman Coulter manufacture 7 Rotor parts subject to wear including but not limited to rotor O rings VTi NVT TLV MLN and TLN rotor tube cavity plugs and gaskets tubing tools optical overspeed disks bear ings seals and lubrication are excluded from this warranty and should be frequently inspected and replaced if they become worn or damaged 8 Keeping a rotor log is not mandatory but may be desirable for maintenance of good laboratory practices Repair
27. tration may need to be reduced Rotor speed is used to control the slope of a CsCl density gradient and must be limited so that CsCl precipitation is avoided Speed and density combinations that intersect on or below the curves in Figure 3 ensure that CsCl will not precipitate during centrifugation in the TLN 100 rotor Curves are provided at two temperatures 20 C black curves and 4 C gray curves 12 TLN 100 Rotor The reference curves in Figure 3 show gradient distribution at equi librium Each curve in Figure 3 is within the density limits allowed for the TLN 100 rotor each curve was generated for a single run speed using the maximum allowable homogeneous CsCl densities one for each fill level that avoid precipitation at that speed The gradients in Figure 3 can be generated from step or linear gradients or from homogeneous solutions But the total amount of CsCl in solu tion must be equivalent to a homogeneous solution corresponding to the concentrations specified in Figure 2 Figure 3 can also be used to approximate the banding positions of sample particles TYPICAL EXAMPLES FOR DETERMINING CsCl RUN PARAMETERS At Speed Floating Components Bands Pelleted Material At Rest in Rotor Upright Example Knowing homogeneous CsCl solution density 1 70 g mL and approximate particle buoyant densities 1 70 and 1 68 g mL where will particles band 1 In Figure 2 find the curve that corresponds to the re
28. uilibrium for the TLN 100 Rotor Centrifugation of homogeneous CsCl solutions at the maximum allowable speeds used to generate each curve is printed along the curve TLN 100 Rotor 15 TLN 100 Rotor 16 2 Select the curve that gives the best particle separation at the required temperature 20 Particles will band at points across the tube diameter where the sketched lines intersect this curve lower axis at equilibrium during centrifugation After centrifuga tion the bands will reorient upper axis 3 Note the run speed and homogeneous CsCI concentration for the selected curve In this example the 1 60 g mL 100 000 rpm curves in Figure 3 give the best separation The separation is located at the center of the tube the particles banding about 1 mm apart at equilibrium during centrifu gation the lower axis However referring to Figure 2 a 1 60 g mL CsCl solution can only be run at 20 C in the TLN 100 rotor If a 4 C run is required the speed must be reduced to 90 000 rpm CARE AND MAINTENANCE MAINTENANCE Threads em Gasket p 342882 Check for Corrosion Plug 368545 gt NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use Regularly lubricate the metal threads in the rotor plugs with a thin even coat of Spinkote lubricant Failure to keep these threa
29. y your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Read the Safety Notice page at the front of this manual before using the rotor 1 Inspect the rotor plugs gaskets spacers and plunger mechanism for damage the high forces generated in this rotor can cause damaged components to fail Use only tubes and accessories listed in Table 1 Check the chemical compatibilities of all materials used refer to Appendix A in Rotors and Tubes For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration 1 Lightly but evenly lubricate metal threads with Spinkote lubricant 306812 Set the rotor into the rotor vise which should be attached to a rigid surface Load the plugged or sealed tubes symmetrically into the rotor see page 8 for tube information If fewer than eight tubes are being run they must be arranged symmetrically in the rotor see Figure 1 Opposing tubes must be filled to the same level with liquid of the same density TLN 100 Rotor OPERATION Plunger Locked Figure 1 Arranging Tubes in the Rotor Two four six or eight tubes can be centrifuged per run if they are arranged in the rotor as shown Use the required spacers see Table 1 to complete the loading operation Insert a rotor plug white gasket end down over each spacer and Screw it in
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