ImmunoComb® - Nordic Lab Supply
Contents
1. Wait 60 minutes for extraction of antibodies Dispense a sample into each well When using the capillary tubes raise and lower the piston several times to achieve mixing When using a pipette mix by depressing the plunger a number of times Proceed to step 5 For control serum open the next 2 consecutive wells Take 5 ul positive control serum C and insert into well A next to the last sample Mix the serum in the well Do the same with the negative control serum in the next well 63BCP103 14 12 04 annm a AAEL EPL i Comb Scale Comb Scale A e Bis O 4 8 b L iC DO o Remove one ImmunoComb from its protective wrapping and insert print side facing you in compartments of Row A Gently move Comb up and down several times then let incubate in Row A s compartments for 10 minutes When using 1 3 or 2 3 of a Comb break the Comb by folding back on notch 4 or 9 respectively Keep the rest in its original sleeve for further use Pierce the cover of the appropriate section of compartment B with the tweezers Follow same procedure for remaining rows at end of each incubation period Gently shake off excess liquid onto a tissue Insert Comb in row B s compartment and let incubate for 2 minutes shake off and transfer Comb to Row C and incubate for 10 minutes Similarly the Comb is placed in Row D for 2 minutes Row E for 2 minutes and Row F for 10 minutes2. ImmunoComb BOVINE CHLAMYDOPHILA SP CHLAMYDIA SP ANTIBODY TEST KIT INSTRUCTION MANUAL SUFFICINT FOR 30 ASSAYS I INTENDED USE This kit is designed to determine cow serum IgG antibody titers for Chlamydophila sp previously known as Chlamydia sp II WHAT IS THE mmunoComb assay The ImmunoComb is a self contained portable kit A sensitive test which detects antibody levels in the blood or serum the ImmunoComb provides results within 35 minutes II HOW DOES THE ImmunoComb work E Based on a solid phase immunoassay principle the ImmunoComb is a plastic card shaped like a comb on which purified Chlamydophila antigen is attached E Either immerse paper disks in cow blood or take a serum specimen Deposit into sample wells of the multi compartment developing plate E Insert Comb into the sample wells so that antibodies from samples bind themselves to the antigens on the Comb E Non bound antibodies are washed out in the second compartment E The next compartment contains an anti cow IgG antibody labeled with an enzyme E Immerse the Comb in this conjugate The bound antibodies will be labeled H Insert the Comb into a compartment where the enzyme reaction takes place This generates a color change which indicates the amount of antibodies present E Using the CombScale convert the spot s color intensity to the anti chlamydophila immunoglobulin level E An internal control the top spot indicates that the
3. allowing the color reaction process to develop After the Comb has completed the cycle for Row F transfer it back to Row E Incubate in Row E for 2 minutes to fix color AIR DRY AND READ RESULTS READING RESULTS WITH THE CombScale A Adjust scale with positive control When the Comb is completely dry align it with the calibrated color CombScale Compare the color resulting from the positive control C sample to the color scale slide the yellow ruler until the C mark appears in the window corresponding to the color FINALLY HOLD THE SLIDE IN THIS POSITION DURING READING B Read each of the spots separately Choose the most suitable color and read the titer in the yellow windows REMEMBER A DIFFERENCE OF ONE COLOR LEVEL WILL NOT AFFECT DIAGNOSIS
4. development is completed E The ImmunoComb may be divided into three separate sections Each segment processes between 1 4 samples IV HANDLING amp STORAGE 1 Store the kit under normal refrigeration 2 8 C 36 46 F Do not freeze the kit 2 Before conducting the test maintain all kit elements and specimens at room temperature preferably for 60 120 minutes or 22 minutes in 37 C Perform assay at room temperature of 20 25 C 68 77 F 3 Avoid spillage and cross contamination of solutions 4 Mix reagents by inverting developing plate several times prior to use 5 Do not mix reagents from different kits or from different compartments of one kit 6 Do not touch teeth of ImmunoComb Card 7 When using developing plate pierce cover of each compartment while strictly following test procedure instructions DO NOT RIP OFF OR REMOVE COVER OF ENTIRE DEVELOPING PLATE ALL AT ONCE 8 The ImmunoComb kit contains inactivated biological material Kit must be handled and disposed of in accordance with accepted sanitary requirements It is recommended to incinerate kit after use Use large amounts of water to flush kit solutions down sewage drainage system 63BCP103 14 12 04 V READING AND INTERPRETING THE RESULTS E To determine the IgG titer of Chlamydophila specimens compare the color intensity of the Comb s appropriate teeth with the color spot series on the enclosed CombScale table see illustratio
5. ns 9 amp 10 for details E The bottom spot on the ImmunoComb tests for Chlamydophila Evaluate the results of each spot separately E Compare the specimen s color intensity with that of the positive control C included in the kit in order to determine its titer E The positive control C is calibrated to a 1 32 titer LF E Specimens with an identical or higher color intensity than the positive control are considered positive E The negative control consists of non immuned sera and should be read as zero S 0 E Specimens with a color intensity lower than the positive control are considered negative or non immuned E To evaluate the titer use the CombScale provided in the kit and determine the titer using Fig A as reference The procedure is detailed in section VI Fig A Relationship between the CombScale s S value and the LF Q a lar COMBSCALE VALUE Important The margin of error is similar to that of other enzyme immunoassay kit procedures Therefore an error in one color scale window will not result in a wrong diagnosis Example of a developed Comb ImmunoComb INTERNAL CONTROL OJ Cat 11BCP100 S LOT a s CHLAMYDOPHILA S N Pee See ee a ee ee 8 9 _ 10 T wo et Sia Se ae se See Ne Ta el TOOTH No RESULT amp REMARKS 15 High positive reaction for Chlamydophila 2 6 Positive reaction for Chlamydophila 3 Verey Low reaction for Chlam
6. ydophila Considered negative 4 10 Negative reaction for Chlamydophila 7 Medium positive reaction for Chlamydophila 8 No internal control development failed 9 High back ground color no valid test ll Positive control 12 Negative control STEP BY STEP wits ImmunoComb The ImmunoComb kit includes A 3 ImmunoComb cards each separately wrapped in an aluminum envelope B 3 developing plates C 3 specimen papers with pre punched disks D One disposable tweezers E One calibrated CombScale color card F One tube of positive control serum and one tube of negative control serum a CombScore sheet and a user manual Perform assay at room temperature of 20 25 C 68 77 F When using a paper disk When using a serum Pierce one of the cow s veins Takea specimen paper and saturate a pre punched disk with the blood Use a pipette or a capillary tube For testing a serum sample use 5 yl Slit open the protective aluminum covering of compartment A with the tweezers one well for each sample Slit open the protective aluminum cover of compartment A with the tweezers one well for each sample A vI When using a paper disk punch out a disk saturated with blood EV Lt Insert the disk into well 1 of compartment A Dip it well into the liquid Proceed with the other samples
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