Mouse anti-Estrogen Receptor, Clone 6F11
Contents
1. biotina LAB SA o un m todo equivalente En general las t cnicas de tinci n inmunohistoqu micas IHQ permiten la visualizaci n de ant genos en cortes de tejido congelados y infiltrados en parafina a trav s de la aplicaci n secuencial de un anticuerpo espec fico al ant geno anticuerpo primario un anticuerpo biotinilado al anticuerpo primario anticuerpo secundario un conjugado enzim tico componente terciario y un sustrato cromog nico con pasos intermedios de lavado La activaci n enzim tica del crom geno resulta en un producto de reacci n visible en el sitio del ant geno La muestra puede ser contra coloreada y cubierta con un cubreobjeto Los resultados se interpretan con un microscopio de luz REACTIVO SUMINISTRADO El anticuerpo Receptor de la Progesterona de rat n se deriva del sobrenadante del cultivo tisular y se diluye en tamp n fosfato PBS Ph 7 4 y seroalb mina bovina al 1 BSA con azida de sodio al 0 1 NaN3 como conservante Inmunog n Prote na recombinante completa que corresponde a la forma alfa del receptor de ER Isotipo IgG kappa Clon 6F11 ALMACENAMIENTO 2 8 C REQUISITOS PARA LA TINCI N INMUNOHISTOQU MICA El prediluido V Line de Invitrogen fue creado para usarse con los sistemas de tinci n inmunohistoqu mica Ventana ES NexES y BenchMark VALIDACION DEL ANALISIS El laboratorio es responsable por la determinaci n de las caracter sticas anal ticas y de rendi2. Inc y no implican ni la aprobaci n ni el aval de Ventana Medical i Systems Inc Las empresas Zymed y Ventana no est n afiliadas asociadas ni relacionadas de manera alguna Para obtener m s informaci n vea la versi n inglesa de la ficha t cnica o vaya a www invitrogen com TIENE ALGUNA PREGUNTA SOBRE ESTE PRODUCTO DIRIJASE A SU DISTRIBUIDOR LOCAL Representante autorizado de IVDD 98 79 EC Invitrogen Ltd PI 08 4214 Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses 6 invitrogen CE Souris anti Recepteur D Estrogenes Clone 6F11 R actif sp cifique l analyte X 08 4214 10 mL Anticorps de pr dilution V Line pr t l emploi Optimis pour les syst mes de coloration immunohistochimique Ventana ES NexES et BenchMark Y UTILISATION VOULUE R actif sp cifique l analyte Les caract ristiques analytiques et de performance ne sont pas tablies PRINCIPE DE LA PROC DURE D IMMUNOHISTOCHIMIE Cet anticorps peut tre u
3. MARCAS Elias JM Am J Clin Pathol 92 62 67 1984 Shi ZR et al J of Histochemistry and Cytochemistry 36 3 317 322 1988 Kumar V et al Cell 51 941 951 1987 Bevitt DJ et al J Pathol 183 2 228 232 1997 College of American Pathologists CAP Certification Program for Immunohistochemistry CAP Northfield IL Http www cap org O Leary T J et al Quality assurance for immunocytochemistry Proposed guideline MM4 P National Committee for Clinical Laboratory Standards NCCLS Wayne PA 1 46 1997 Clinical Laboratory Improvement Amendments of 1988 Final Rule 57 FR 7163 February 28 1992 Elias J M et al Special Report Quality Control in immunohistochemistry Am J Clin Path 92 836 1989 Kiernan J A Histological and Histochemical Methods Theory and Practice New York Pergamon Press 1981 Sheehan D C and Hrapchak B B Theory and Practice of Histotechnology St Louis C V Mosby Co 1980 Taylor and Cote Immunomicroscopy A Diagnostic Tool for the Surgical Pathologist 2 Ed W B Saunders Co Philadelphia 1994 COMERCIALES Cap Plus Clearmount Digest AIITM HistoGrip Histomount y Histostain NBA PicTure y Zymed son marcas comerciales de Zymed Laboratories Inc Ventana ES NexES y BenchMark son marcas comerciales de Ventana Medical Systems Inc o x ER eee Los anticuerpos V Line son elaborados exclusivamente por Zymed Laboratories
4. et Histostain NBA PicTure et Zymed sont des marques des Zymed Laboratories Inc Ventana ES NexES et BenchMark sont des marques d pos es de Ventana Medical Systems Inc Les anticorps V Line sont d velopp s exclusivement par Zymed Laboratories Inc et n insinuent pas une approbation ou un soutien de la part de Ventana Medical Systems Inc Zymed et Ventana ne sont en aucune fa on affili es ou associ es Pour des informations plus d taill es veuillez vous r f rer aux feuilles de donn es en version anglaise ou aller sur www invitrogen com SI VOUS AVEZ DES QUESTIONS SUR CE PRODUIT CONTACTEZ VOTRE DISTRIBUTEUR LOCAL Repr sentant Autoris pour IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4214 Rev 08 08 DCC 08 1089 O 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE Topo anti Recettore Degli Estrogeni Clone 6F11 Reagente analita specifico X 08 4214 10 mL Anticorpo prediluito V Line Pronto per l uso Ottimizzato per i sistemi di colorazione immunoi
5. Isotipo IgG kappa Clone 6F11 CONSERVAZIONE 2 8 C REQUISITI PER LA COLORAZIONE IMMUNOISTOCHIMICA L Anticorpo diluito V Line di Invitrogen stato sviluppato per l uso con i sistemi di colorazione immunoistochimica Ventana ES NexES e BenchMark CONVALIDA DELLE PROVE Ciascun singolo laboratorio dovr determinare le caratteristiche analitiche e della performance delle prove diagnostiche eseguite utilizzando il presente reagente analita specifico e quindi convalidare l uso di dette prove Sono disponibili le seguenti fonti di informazioni sulla convalida delle prove ed il relativo utilizzo Programma di certificazione per l immunoistochimica CAP Linea guida sull IIC del NCCLS e Emendamenti di perfezionamento delle procedure cliniche di laboratorio del 1988 Nel materiale di consultazione sono rinvenibili ulteriori informazioni sulla colorazione immunoistochimica www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4214 Rev 08 08 DCC 08 1089 O 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses CARICAMENTO DELL EROGATORE DEFINITO DALL UTENTE Invitrogen raccomanda di osservare la seg
6. 6 invitrogen CE Mouse anti Estrogen Receptor Clone 6F11 Lot No X 08 4214 10 mL V Line Predilute Ready to Use Antibody INTENDED USE Analyte Specific Reagent Analytical and performance characteristics are not established PRINCIPLE OF IMMUNOHISTOCHEMISTRY PROCEDURE This antibody may be used as the primary antibody in immunohistochemistry applications In general immunohistochemical IHC staining techniques allow for the visualization of antigens in frozen and paraffin embedded tissues via the sequential application of a specific antibody to the antigen primary antibody a biotinylated antibody to the primary antibody secondary antibody an enzyme conjugate tertiary component and a chromogenic substrate with interposed washing steps The enzymatic activation of the chromogen results in a visible reaction product at the antigen site The specimen may then be counterstained and coverslipped Results are interpreted using a light microscope REAGENT PROVIDED This monoclonal antibody is derived from tissue culture supernatant purified The vial is filled to 10 mL with reagent containing PBS 1 BSA and 0 1 sodium azide Immunogen Recombinant full length alpha form of ER protein Total protein concentration g L i 4 a Clone 6F11 Isotype IgG1 kappa Antibody concentration mg L STORAGE 2 8 C REQUIREMENTS FOR IMMUNOHISTOCHEMISTRY STAINING Invitrogen s V Line Predilute was developed for use with the Ven
7. Immunomicroscopy A Diagnostic Tool for the Surgical Pathologist 2 Ed W B Saunders Co Philadelphia 1994 nas e ES ci an MARCHI REGISTRATI Cap Plus Clearmount Digest AIITM HistoGrip Histomount e Histostain NBA PicTure e Zymed sono marchi registrati di Zymed Laboratories Inc Ventana ES NexES e BenchMark sono marchi commerciali di proprieta di Ventana Medical Systems Inc Gli anticorpi V Line sono sviluppati esclusivamente da Zymed Laboratories Inc e non implicano alcuna autorizzazione o approvazione da parte di Ventana Medical Systems Inc Zymed e Ventana non sono imprese consociate affiliate o collegate in alcun altro modo Per informazioni pi dettagliate vi preghiamo di consultare la versione inglese del catalogo oppure visitate il sito www invitrogen com SE AVETE DOMANDE SUL PRODOTTO CONTATTATE IL VOSTRO DISTRIBUTORE PIU VICINO Rappresentante Autorizzato per IVDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4214 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and condi
8. New York Pergamon Press 1981 Sheehan D C and Hrapchak B B Theory and Practice of Histotechnology St Louis C V Mosby Co 1980 Taylor and Cote Inmunomicroscopy A Diagnostic Tool for the Surgical Pathologist 2 Ed W B Saunders Co Philadelphia 1994 ABS me oon Authorized Representative for VDD 98 79 EC Invitrogen Ltd Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4214 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses e invitrogen CE Anticuerpo Receptor de Estr geno de rat n Clon 6F11 Reactivo espec fico para electrolito X 08 4214 10 mL Anticuerpo prediluido V Line listo para usar Optimizado para los sistemas de tinci n inmunohistoqu mica Ventana ES NexES y BenchMark PROP SITO DE USO Reactivo espec fico para electrolito Las caracter sticas anal ticas y de rendimiento no han sido determinadas PRINCIPIO DEL PROCEDIMIENTO DE INMUNOHISTOQU MICA Este anticuerpo puede ser utilizado como el anticuerpo primario con el m todo de detecci n marcado con estreptavidina
9. d tails suppl mentaires 1 Retirer le bouchon l extr mit de la seringue de transfert Ventana retirer ensuite compl tement le piston du corps de pompe et remettre le bouchon dans le corps de pompe de la seringue 2 Marquer de fa on appropri le r actif Ventana et la seringue de transfert avec l anticorps de Invitrogen utilis 3 Verser le flacon de pr dilution V Line inclus 10 mL anticorps pr t l emploi dans le corps de pompe de la seringue de transfert de Ventana ensuite remettre le piston dans le corps de pompe 4 Retourner la seringue de transfert de Ventana le bouchon tourn vers le haut et le piston vers le bas Retirer ensuite le bouchon Chasser l air de la seringue de transfert Ventana en poussant doucement le piston vers le bouchon Retirer ensuite le bouchon de l extr mit de la seringue de r actif Ventana Attacher l embout de la seringue de r actif Ventana sur l embout de la seringue de transfert Ventana Faire passer l anticorps de la seringue de transfert Ventana dans la seringue de r actif Ventana La quantit maximale contenue dans la seringue de r actif Ventana est de 12 5 mL ce qui permet d effectuer environ 120 tests 9 Poser l amp tiquette de Invitrogen ci jointe sur la seringue de r actif de Ventana e AA L anticorps de pr dilution V Line de Invitrogen est pr t tre utilis avec les syst mes de coloration immunohistochimique de Ven
10. gende Invitrogen Etikett auf der Ventana Reagenzspritze anbringen Der Invitrogen V Line Vorverd nnungs Antik rper kann jetzt mit den immunhistochemischen F rbungssystemen Ventana ES NexES und BenchMark eingesetzt werden Spezifische Details zum Betrieb und zur Ereignissequenz der immunhistochemischen F rbungssysteme Ventana ES NexES und BenchMark sind den entsprechenden Ventana Benutzerhandb chern zu entnehmen NA PIN 10 O0 1 Elias JM Am J Clin Pathol 92 62 67 1984 Shi ZR et al J of Histochemistry and Cytochemistry 36 3 317 322 1988 Kumar V et al Cell 51 941 951 1987 Bevitt DJ et al J Pathol 183 2 228 232 1997 College of American Pathologists CAP Certification Program for Immunohistochemistry CAP Northfield IL Http www cap org O Leary T J et al Quality assurance for immunocytochemistry Proposed guideline MM4 P National Committee for Clinical Laboratory Standards NCCLS Wayne PA 1 46 1997 Clinical Laboratory Improvement Amendments of 1988 Final Rule 57 FR 7163 February 28 1992 Elias J M et al Special Report Quality Control in immunohistochemistry Am J Clin Path 92 836 1989 Kiernan J A Histological and Histochemical Methods Theory and Practice New York Pergamon Press 1981 Sheehan D C and Hrapchak B B Theory and Practice of Histotechnology St Louis C V Mosby Co 1980 Taylor and Cote Immunomicroscopy A Diagnostic Tool for the Surgical Path
11. irca 9 Attaccare l etichetta acclusa Invitrogen sulla siringa per reagenti Ventana posta Mi L anticorpo prediluito V Line di Invitrogen fornito pronto per l uso ed da utilizzarsi con i sistemi di colorazione immunoistochimica Ventana ES NexES e BenchMark Per informazioni particolareggiate sul funzionamento e la sequenza procedurale dei sistemi di colorazione immunoistochimica Ventana ES NexES e BenchMark si prega di consultare i rispettivi Manuali per l utente Ventana Elias JM Am J Clin Pathol 92 62 67 1984 Shi ZR et al J of Histochemistry and Cytochemistry 36 3 317 322 1988 Kumar V et al Cell 51 941 951 1987 Bevitt DJ et al J Pathol 183 2 228 232 1997 College of American Pathologists CAP Certification Program for Immunohistochemistry CAP Northfield IL Http www cap org O Leary T J et al Quality assurance for immunocytochemistry Proposed guideline MM4 P National Committee for Clinical Laboratory Standards NCCLS Wayne PA 1 46 1997 Clinical Laboratory Improvement Amendments of 1988 Final Rule 57 FR 7163 February 28 1992 Elias J M et al Special Report Quality Control in immunohistochemistry Am J Clin Path 92 836 1989 Kiernan J A Histological and Histochemical Methods Theory and Practice New York Pergamon Press 1981 Sheehan D C and Hrapchak B B Theory and Practice of Histotechnology St Louis C V Mosby Co 1980 Taylor and Cote
12. les syst mes de coloration immunohistochimique Ventana ES NexES et BenchMark VALIDATION DE L ESSAI Il incombe au laboratoire d tablir les caract ristiques analytiques et de performance de tout essai de diagnostic utilisant ce r actif sp cifique a l analyte et de valider Putilisation de cet essai Les sources d information suivantes concernant la validation et l utilisation de l essai sont disponibles programme de certification CAP pour l immunohistochimie la directive concernant l IHC de NCCLS et les modifications d am lioration du laboratoire clinique de 1988 Vous trouverez des donn es suppl mentaires concernant l immunohistocoloration dans les r f rences www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4214 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses CHARGEMENT DU DISTRIBUTEUR TEL QUE D FINI PAR L UTILISATEUR Invitrogen recommande l utilisation de la proc dure ci apr s pour remplir les distributeurs de r actif Ventana avec la pr dilution V Line de Invitrogen Se reporter au manuel de l utilisateur de Ventana pour des
13. m By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses F LLEN DES VOM BENUTZER DEFINIERTEN DISPENSERS Invitrogen empfiehlt die folgende Methode zum F llen der Ventana Reagenz Dispenser mit Invitrogen V Line Vorverd nnung Weitere Anweisungen sind dem Ventana Benutzerhandbuch zu entnehmen 1 PLAN 9 Den Verschluss am Ende der Ventana Transferspritze entfernen dann den Kolben ganz aus dem Zylinder herausziehen und den Verschluss wieder auf dem Spritzenzylinder anbringen Die Ventana Reagenz und Transferspritze entsprechend mit dem verwendeten Invitrogen Antik rper kennzeichnen Das beiliegende Fl schchen mit V Line Vorverdiinnung 10 mL gebrauchsfertiger Antik rper in den Zylinder der Ventana Transferspritze geben und dann den Kolben wieder in den Zylinder schieben Die Ventana Transferspritze invertieren Dazu den Verschluss nach oben und den Kolben nach unten richten Dann den Verschluss entfernen Zum Entliiften der Ventana Transferspritze den Kolben vorsichtig in Richtung Verschluss schieben Dann den Verschluss von der Spitze der Ventana Reagenzspritze abnehmen Die Ventana Reagenzspritzend se an der Ventana Transferspritzend se befestigen Antik rper aus der Ventana Transferspritze in die Ventana Reagenzspritze spritzen Das maximale Fassungsverm gen der Ventana Reagenzspritze betr gt 12 5 mL und reicht f r etwa 120 Tests Das beilie
14. miento de cualquier an lisis diagn stico mediante este reactivo espec fico para electrolitos y por la validaci n del uso de dicho an lisis Se dispone de las siguientes fuentes de informaci n sobre la validaci n y uso del an lisis Programa de certificaci n CAP para inmunohistoquimica las recomendaciones sobre IHQ del NCCLS y las modificaciones para mejor a del laboratorio cl nico 1988 En las referencias puede encontrarse informaci n adicional sobre inmunotinci n histol gica www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4214 Rev 08 08 DCC 08 1089 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses CARGA DEL DISPENSADOR DEFINIDO POR EL USUARIO Invitrogen recomienda el siguiente procedimiento para llenar los dispensadores de reactivo Ventana con el prediluido V Line de Invitrogen Consulte el Manual del Usuario de Ventana para obtener m s informaci n 1 2 W BOSON e ES 9 Retire el tap n del extremo de la Jeringa de transferencia Ventana luego retire el mbolo por completo del cilindro y vuelva a colocar el tap n en el cilindro de la jeringa Coloque una etiq
15. ologist 2 Ed W B Saunders Co Philadelphia 1994 WARENZEICHEN Cap Plus Clearmount Digest All HistoGrip Histomount und Histostain NBA PicTure und Zymed sind Warenzeichen der Zymed Laboratories Inc Ventana ES NexES und BenchMark sind Marken der Ventana Medical Systems Inc V Line Antik rper werden ausschlieBlich von Zymed Laboratories Inc entwickelt und wurden nicht durch Ventana Medical Systems Inc genehmigt oder gef rdert Zymed und Ventana sind in keiner Weise angegliedert aneinander angeschlossen bzw miteinander verbunden Fiir genauere Information beziehen Sie sich bitte entweder auf die englische Version auf der Datenplatte oder auf www invitrogen com SOLLTEN SIE IRGENDWELCHE FRAGEN BER DIESES PRODUKT HABEN WENDEN SIE SICH BITTE AN IHREN ORTLICHEN VERTEILER Bevollm chtigter Repr sentant f r IVDD 98 79 EC Invitrogen Ltd PI 08 4214 Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com Rev 08 08 DCC 08 1089 O 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses
16. rd nnt in Phosphatgepufferter Salzl sung PBS pH 7 4 und 1 igem Rinder Serum Albumin BSA mit 0 1 Sodium Azid NaN als ein Konservierungsmittel Immunogen Rekombinantes Protein mit voller L nge entspricht der Alpha Form des humanen Ostrogenrezeptors Isotyp IgG kappa Klon 6F11 LAGERUNG 2 8 C VORAUSSETZUNGEN FUR DIE IMMUNHISTOCHEMISCHE FARBUNG Invitrogens V Line Vorverdiinnung wurde speziell fiir die Verwendung mit den immunhistochemischen Farbungssystemen Ventana ES NexES und BenchMark ASSAY VALIDIERUNG Das Labor ist dafiir verantwortlich die analytischen und Leistungsmerkmale jedes Diagnose Assays unter Verwendung dieses analytspezifischen Reagenz zu bestimmen und die Verwendung des Assay zu validieren Folgende Informationsquellen tiber die Validierung und Verwendung des Assay stehen zur Verfiigung CAP Zertifizierungsprogramm f r Immunhistochemie die NCCLS IHC Richtlinie und die Clinical Laboratory Improvement Amendments von 1988 Weitere Informationen iiber Immunhistochemische F rbung sind dem entsprechenden Referenzmaterial zu entnehmen www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PI 08 4214 Rev 08 08 DCC 08 1089 O 2007 Invitrogen Corporation All right reserved These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or www invitrogen co
17. stochimica Ventana ES NexES e BenchMark SCOPI D UTILIZZO Reagente analita specifico Le caratteristiche analitiche e della performance non sono state stabilite PRINCIPIO DI FUNZIONAMENTO DELLA PROCEDURA IMMUNOISTOCHIMICA Questo anticorpo pu essere usato quale anticorpo primario nel metodo di rilevazione marcato con streptavidina biotina LAB SA o metodi analoghi In termini generali le tecniche di colorazione immunoistochimica IIC consentono la visualizzazione di antigeni in tessuti congelati ed inclusi in paraffina tramite l applicazione sequenziale di un anticorpo specifico sull antigene anticorpo primario di un anticorpo biotilinato sull anticorpo primario anticorpo secondario un coniugato enzimatico componente terziario ed un substrato cromogenico con fasi di lavaggio frapposte L attivazione enzimatica del cromogeno induce un prodotto di reazione visibile presso il sito dell antigene Il provino pu quindi essere controcolorato ed inserito in un coprivetrino I risultati possono essere interpretati usando un microscopio ottico REAGENTI FORNITI Topo anti Recettore Progesterone derivato da supernatante di coltura di tessuto e diluito in soluzione salina tamponata al fosfato PBS pH 7 4 e 1 di albumina di siero bovino BSA con 0 1 sodio azide NaN3 come conservante Immunogeno Proteina ricombinante di lunghezza completa corrispondente alla forma alfa del ricettore dell estrogeno umano
18. tana ES NexES et BenchMark Pour des d tails sp cifiques concernant le fonctionnement et la s quence des v nements des syst mes de coloration immunohistochimique de Ventana EST NexES et BenchMark se reporter au manuel de l utilisateur Ventana en question Elias JM Am J Clin Pathol 92 62 67 1984 Shi ZR et al J of Histochemistry and Cytochemistry 36 3 317 322 1988 Kumar V et al Cell 51 941 951 1987 Bevitt DJ et al J Pathol 183 2 228 232 1997 College of American Pathologists CAP Certification Program for Immunohistochemistry CAP Northfield IL Http www cap org O Leary T J et al Quality assurance for immunocytochemistry Proposed guideline MM4 P National Committee for Clinical Laboratory Standards NCCLS Wayne PA 1 46 1997 Clinical Laboratory Improvement Amendments of 1988 Final Rule 57 FR 7163 February 28 1992 Elias J M et al Special Report Quality Control in immunohistochemistry Am J Clin Path 92 836 1989 Kiernan J A Histological and Histochemical Methods Theory and Practice New York Pergamon Press 1981 Sheehan D C and Hrapchak B B Theory and Practice of Histotechnology St Louis C V Mosby Co 1980 Taylor and Cote Immunomicroscopy A Diagnostic Tool for the Surgical Pathologist 2 Ed W B Saunders Co Philadelphia 1994 NA PL D ES me 00 MARQUE DE FABRIQUE Cap Plus Clearmount Digest AIITM HistoGrip Histomount
19. tana ESTM NexES and BenchMark Immunohistochemistry Staining Systems Refer to the applicable Operator s Manual for further information ASSAY VALIDATION It is the laboratory s responsibility to establish the analytical and performance characteristics of any diagnostic assay using this analyte specific reagent and to validate the use of such assay The following sources of information about assay validation and use are available CAP Certification Program for Immunohistochemistry the NCCLS IHC guideline and the Clinical Laboratory Improvement Amendments of 1988 Additional information about immunohistostaining can be found in references 4 REFERENCES Elias JM Am J Clin Pathol 92 62 67 1984 Shi ZR et al J of Histochemistry and Cytochemistry 36 3 317 322 1988 Kumar V et al Cell 51 941 951 1987 Bevitt DJ et al J Pathol 183 2 228 232 1997 College of American Pathologists CAP Certification Program for Immunohistochemistry CAP Northfield IL Http www cap org O Leary T J et al Quality assurance for immunocytochemistry Proposed guideline MM4 P National Committee for Clinical Laboratory Standards NCCLS Wayne PA 1 46 1997 Clinical Laboratory Improvement Amendments of 1988 Final Rule 57 FR 7163 February 28 1992 Elias J M et al Special Report Quality Control in immunohistochemistry Am J Clin Path 92 836 1989 Kiernan J A Histological and Histochemical Methods Theory and Practice
20. tilis comme anticorps primaire avec la m thode de d tection tiquet e streptavidine biotine LAB SA ou une m thode quivalente En g n ral les techniques de coloration immunohistochimiques IHC permettent de visualiser des antig nes dans des tissus congel s et incorpor s dans de la paraffine par l interm diaire de V application s quentielle d un anticorps sp cifique l antig ne anticorps primaire un anticorps biotinyl l anticorps primaire anticorps secondaire un conjugu enzymatique composant tertiaire et un substrat chromog nique avec des tapes de lavage interpos es L activation enzymatique du chromog ne produit une r action visible du produit au site de l antig ne Le sp cimen peut alors tre contre color et recouvert d une lamelle couvre objet Les r sultats sont interpr t s l aide d un microscope optique REACTIF FOURNI Souris anti Recepteur de Progesterone est d riv d un surnageant d une culture de tissu et dilu dans du Phosphate Tamponn Salin PBS pH 7 4 et 1 d albumine de s rum de bovin BSA avec 0 1 azide de sodium NaN3 comme pr servatif Immunog ne Prot ine recombinante pleine longueur correspondant la forme alpha de r cepteur d estrogene humain Isotype IgG kappa Clone 6F11 STOCKAGE 2 8 C MAT RIEL N CESSAIRE POUR LA COLORATION IMMUNOHISTOCHIMIQUE La pr dilution V Line de Invitrogen a t concue pour tre utilis e avec
21. tions of all applicable Limited Use Label Licenses 6 invitrogen CE Maus Anti Estrogen Rezeptor Klon 6F11 Analytspezifisches Reagenz X 08 4214 10 mL V Line Vorverdiinnung gebrauchsfertiger Antikorper Optimiert f r die immunhistochemischen F rbungssysteme Ventana ES NexES und BenchMark BEABSICHTIGTER NUTZEN Analytspezifisches Reagenz Analytische und Leistungsmerkmale wurden nicht festgelegt IMMUNHISTOCHEMISCHES VERFAHRENSPRINZIP Dieser Antik rper kann als prim rer Antik rper mit der gekennzeichneten Streptavidin Biotin LAB SA Detektion oder einer gleichwertigen Methode verwendet werden Generell erm glichen immunhistochemische IHC F rbungsmethoden die Visualisierung der Antigene in gefrorenem und in Paraffin eingebetteten Geweben durch die sequenzielle Anwendung eines spezifischen Antik rpers gegen das Antigen prim rer Antik rper eines biotinylierten Antik rpers gegen den prim ren Antik rper sekund ren Antik rper eines Enzymkonjugats terti re Komponente und eines chromogenen Substrats mit zwischengeschalteten Waschschritten Die enzymatische Aktivierung des Chromogens f hrt zu einer sichtbaren Reaktion an der Antigenstelle Die Probe kann anschlie end gegengef rbt und mit einem Coverslip versehen werden Die Ergebnisse werden unter Verwendung eines Lichtmikroskops interpretiert REAGENS GELIEFERT Maus Anti Progesterone Rezeptor ist wird aus Gewebekultur Supernatant abgeleitet und ve
22. uente procedura per il riempimento degli erogatori di reagente Ventana con l anticorpo prediluito V Line di Invitrogen Per ulteriori chiarimenti si prega di consultare il Manuale per l utente Ventana 1 Rimuovere il tappo posto sull estremit della siringa di trasferimento Ventana quindi estrarre completamente lo stantuffodal cilindro e rimettere il tappo sul cilindro della siringa 2 Etichettare debitamente la siringa per reagenti e la siringa di trasferimento Ventana specificando il nome dell anticorpo Invitrogen utilizzato 3 Versare il contenuto della boccetta acclusa di anticorpo prediluito V Line di Invitrogen 10 mL anticorpo pronto per l uso nel cilindro della siringa di trasferimento Ventana quindi reinserire lo stantuffo nel cilindro 4 Capovolgere la siringa di trasferimento Ventana in modo che il tappo sia rivolto verso l alto e lo stantuffo verso il basso Quindi rimuovere il tappo Eliminare l aria dalla siringa di trasferimento Ventana spingendo cautamente lo stantuffo verso il tappo A questo punto rimuovere il tappo dalla punta della siringa di trasferimento Ventana Collegare l ugello della siringa per reagenti Ventana all ugello della siringa di trasferimento Ventana Erogare l anticorpo dalla siringa di trasferimento Ventana alla siringa per reagenti Ventana La siringa per reagenti Ventana ha una capacit massima di 12 5 mL ovvero un quantitativo sufficiente per eseguire 120 prove c
23. ueta a la Jeringa de reactivo y transferencia Ventana indicando el anticuerpo Invitrogen utilizado Vierta la ampolla que se adjunta de prediluido V Line 10 mL anticuerpo listo para usar en el cilindro de la Jeringa de transferencia Ventana luego vuelva a colocar el mbolo en el cilindro Invierta la Jeringa de transferencia Ventana colocando el tap n hacia arriba y el mbolo hacia abajo Luego retire el tap n Haga salir el aire de la Jeringa de transferencia Ventana empujando cuidadosamente el mbolo hacia el tap n Ahora retire el tap n de la punta de la Jeringa de reactivo Ventana Conecte la boquilla de la Jeringa de reactivo Ventana a la boquilla de la Jeringa de transferencia Ventana Dispense anticuerpo de la Jeringa de transferencia Ventana hacia la Jeringa de reactivo Ventana La cantidad m xima que puede contener la Jeringa de reactivo Ventana es 12 5 mL lo cual es suficiente para unas 120 pruebas Coloque la etiqueta Invitrogen adjunta en la Jeringa de reactivo Ventana El anticuerpo prediluido V Line de Invitrogen est listo para usar con los sistemas de tinci n inmunohistoqu mica Ventana EST NexES y BenchMark Para obtener detalles espec ficos sobre el funcionamiento y la secuencia de procedimientos de los sistemas de tinci n inmunohistoqu mica Ventana ES NexES y BenchMark consulte los manuales del usuario correspondientes y respectivos nus ONS 7 8 9 10 Il
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