ジーンキューブ MAC - 医薬品医療機器総合機構
Contents
1.2. 2015 9 10 7 Solution L 15
3. 2 3 10 24 NQ MACGC_PS150910
4. MAC PC 15 MAC NC 13000xg 4 C 15 NN 2 0 A 6 OS 8 MAC 3 400 1 5mL 2 1mL 99 59 GENECUBE 4 80C
5. NALC NaOH 2 MAC MAC DNA 3 MAC 1 1
6. 6 6 NALC 0 5g 24 5 i 0 Polymerase Chain Reaction PCR QProbe 2 a a 5 gt 1 Mycobacterium avium complex MAC Mycobacterium avium Mycobacterium 35 0 0 0 sec ntracellulare DNA GENECUBE ISOPLANT x MAC DnaJ
7. TOYOBO ldeas amp Chemistry TEL 06 6348 530 8230 2 8 3335 FAX 06 6348 3833 104 6004 8 10 03 6219 7608 2 3 MTB MAC MAC Mix 3 KOD Mix IC Mix
8. 4 a 2 TE 5 MAC NC 20 5 GENECUBE ww SS INN 4 Ba gt dNTP 5 1 lt 8 6 1 5 _ 6 GENECUBE 2 XX2 3
9. 0 5mL MAC R 9 SN 5 MAC QProbe Me MMS 1 3 3g IC Mix 1gL Na a a KOD Mix 3 L MAC Mix 3rL 1mL pHH6 8 3 IC Mix RN ie 2 4 E7 y i SN 4 MAC PC HH Hd
10. 13000 xg 4C 10 709 10mmol L Tris HCI pH7 5 20 2 GENECUBE 1 3 8 SAP NALC NaOH MAC2 M avium M int
11. 2 10 2 5 SAP Ny EE aN KR HY 1 gt Se gt y 50mL 1mL 15 2 5mL SS 02SZ mL NALC NaOH EOE Vortex 20 5 00 9 NALC NaOH GENECUBE
12. PCR 1 PCR 138 92 8 128 138 1 1 N95 2 3 5 4 5
13. 6 0 5 7 8 121 20 2 0 5 1 1
14. 6 50 0 MAC DNA 300 80 10 1 000xg 3 0 2 2 a 1 GENECUBE 5 ene on KOD DNA RR RC 2 QProbe RN RA B 94 0 30 0 sec GENECUBE 0 390 30 0 se GENECUBE SR eR OO 40 0 75 0C 0 09 sec
15. MAC F MAC R 0 see 302 5 40 SNS 98 0 1 0 sec KOD DNA 60 0 3 0 GENECUBE gt Wg dNTP 30 300 1 5mL Eh 4 MAC MAC QProbe
16. a i B En Fr wp 5 RN 1 20 25 15 0 90 0 GENECUBE EAS OR KOD DNA 50mL pH6 8 8 3 GENECUBE 3 dNTP 1 2 R 0 MAC Mix 3000 Xg 4 20 2 MAC PC Ne NC MAC F
17. gt SOPLANT OES PCR MAC_DNA 1 5mL KOD Mix 1C Mr I RN 0 WE MTB MAC 100 GQProbe SolutionT 300 Vortex 1 2 A5246K 1 GENECUBE imvalid
18. 1 Invalid 2 MAC PC MAC NC KJ 1 a 100 100 c DNA d
19. MAC DnaJ DNA 2 E 1 3 1 3 i F 3 1 4 i yi 1 4 4 15 GENECUBE 5 MAC DnaJ DNA v 2 FT
20. 1 2 8C MAC 140 KOD Mix XX6 140 MAC Mix X6 300 IC Mix 1 300 E MAC PC 1 300 EF MAC NC 1 1 Yamada Noda M et al Mycobacterium species identification A new approach via ja gene sequencing Syst Appl Microbiol 2007 Sep 30 6 453 462 2 Kitabayashi M et g Gene Cloning and Polymerase Chain Reaction with Proliferating Cell Nuclear Antigen from Thermococcus kodakaraensis KOD1 Biosci Biotechnol Biochem 2002 Oct 66 10 2194 2200 3 Kurata S ez al Fluorescent quenching based quantitative detection of specific DNA RNA using a BODIPY R FL labeled probe or primer Nucleic Acids Res 2001 Mar 15 29 6 e34 4 Torimura M et al Fluorescence Quenching Phenomenon by Photoinduced Electron Transfer between a F 155 160 uorescent Dye and a Nucleotide Base Anal Sci 2001 Jan 17 1 5 2007 2007 31 36
21. 138 1 98 6 2 92 8 1 67 1 1 1 2 69 1 2 138 98 6 136 138 2 65 3 7 63 3 2 PCR 1 PCR 4 3 PCR 3
22. 510nm 550nm 573nm 613nm 3 NALC NaOH DNA 49 50mnL lt 7 MAC PC MAC NC O 0 Imol L 2 994 50mL 3 6 O SPY 4 N 121 15 USA 8 ON AN AN Ns ae 4
23. 0 Vortex 5 6 2015 1 21 6 1 1 22200AMX00913000 87749 1 DNA 50 15 KOD Mix 2 MAC Mix Solution 150L Vortex 1 2 IC Mix
24. racellulare DNA 0 5mL NALC NaOH MAC 39 DNA SDS 4 SDS
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