Mo2-RD1
Contents
1.2. 10 000 L PBS 10 000 1 5 L 1 200L PBS BSA0 19 NBS 296 0 196 BSA NBS
3. Mo2 CD14 MY4 CD14 T B CD2 CD3 CD5 CD19 CD21 CD14 GPI GPI PNH CD14 Ficoll paque AB Fc BSA NBS PBS CD14
4. 2 FAB TCR lgH
5. 2 PBS 8 09 L 2 12 2 9gL 1 1 0 2gL 0 2gL pH7 2 7 4 BSA 0 19 0 196 3 MSlgM RD1 6602940 100 Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL a Acridine orange Baker A366 3 0 005mg mL Ficoll paque Pharmacia 17 0840 03 1 EDTA 3 10 x 10 L 10 x 10 L 3x10 L
6. T non T Mo2 RD1 CD14 CD14 55kDa LPS LPB Mo2 RD1 CD14 FAB AML M4 M5 45 1009 6 Mo2 AML M1 M2 3496 Mo2
7. FS 90 SS 1 4 1 Ro EDTA 6 90 85 Ficoll paque Ficoll paque
8. PBS 2 4 15 25 6 a gt 10 x 10 L 10 20 x10 2 20 30 x10 3 30 40 x103 5 40 60 x10 6 60 100 x103 10 100 200 x103 20 37 C PBS b 3 x 10 L 1 25C 500 x g 1 700rpm 5 2 3 4 LH 700 5 10x10 mm 1 100L
9. 5 Ficoll paque
10. 58 84 CD14 MY4 116 Mo2 BALB c P3 NS1 1 AG4 1 lg lgM H z L Mo2 RD1 Phycoerythrin PE PE Protein 0 5 1 5 468 575nm 568 590nm 1 0 296 0 01M 0 15M 0 196 CD14 488nm RD1
11. 2 20 25C 3 Mo2 RD1 6603266 100 Bernard A Boumsell L Dausset J Milstein C Schlossman SF Leukocyte_ Typing 1984 Berlin Springer Verlag 108 407 2 McMichael AJ Leukocyte Typing _ White Cl Differentiation Antigens 1987 Oxford Oxford University Press 586 589 3 Todd RF van Agthoven A Schlossman SF Terhorst C 1982 Structural analysis of differentiation antigens Mol and Mo2 on human monocytes Hybridoma 1 329 337 4 Todd RF Nadler LM Schlossman SF 1981 Antigens on human monocytes identified by monoclonal antibodies J Immunol 126 1435 1442 5 Todd RF Schlossman SF 1982 Analysis of antigenic determinants on human monocytes and macrophages Blood 59 775 786 6 Todd RF Griffin JD Ritz BJ Nadler LM Abrams T Schlossman SF 1981 Expression of normal mon
12. 2 Ficoll paque 1 4mL PBS 2 Ficoll paque 4mL 1 3 400 xg 30 4 Ficoll paque 5 PBS 4 400xg 8 6 PBS 4 C 400 x g 4 7 PBS 4C 400xg 3 8 PBS 1x10 xL 1 x 10 mL 9 Fc 10 15 AB 196 vV 10 a b
13. 3 100 85 12 13 14 15 1 1 2 3 4 5 6 7 8 9 10 12mm x 75mm 100L 200 L IgM MslgM RD1 45 a b a
14. 90 a 11 1 x10 1x10 L 1mL 12 PBS 4 C 400 xg 4 13 0 05mg mL Propidium lodide 3 1 14 PBS 4 C 400 xg 4 15 14 16 859 b 11 25 000 1x 10 L 25L Propidium lodide 0 01mg mL 10 x L 30 Acridine orange 0 005mg mL 10L
15. 1 1 2 1 6603152 300 PBS 24mL 1mL 2 1L 8 26g 1 0g EDTA4 2 37mg pH7 2 gt 7 4 1 1 2 30 2 10 15 20 30
16. 6603266 2009 11 2007 2 20900AMY00141000 877429 Mo2 RD1 IN ww 1 CD14 55KD CD14
17. AML M3 M6 ALL Mo2 Mo2 CD14 CD14 Mo2 RD1 959 Mo2 RD1 59 Mo2 RD1 CD14 Mo2 RD1 y 0 99X 0 40 r 0
18. 2mL 5 15 b PBS 400 x g 5 1mL 30 2 250 L PBS 4 C 400 xg 5 PBS EPICS Cytomics 2 Ficoll paque 1 2 3 4 5 6 7 8 12mm x 75mm Ficoll paque
19. 4 C 400xg 5 200L IgM MslgM RD1 4 C 30 4 C 400 xg 5 3 4 PBS 200L 4 C 400 xg 5 3 4 PBS 200L EPICS Cytomics 3 4 1
20. 1 x 10 1x10 L 1mL 4 C 400 x g 4 200L IgM MslgM RD1 4 C 30 PBS 1mL 4C 400 x g 4 6 2 PBS EPICS Cytomics 3 Ficoll paque 1 2 3 4 5 6 7 8 9 10 1 x 10 200L Ficoll paque 200 L U 96
21. 1 2 2 2 1 Mo2 RD1 Ficoll paque 200 uxL PBS 1096 20L 2 1 3 CD14 x100 Fc
22. 88 The CD14 monocyte TCY062 07 10 differentiation antigen maps to a region encoding growth factors and receptors Science 239 497 500 15 Medeiros LJ Herrington RD Gonzalez CL Jaffe ES Cossman J 1991 My4 antibody staining of non Hodgkin s Lymphomas Am J Clin Pathol 95 363 368 16 Tasies D Montserrat E Reverter JC Villamor N Rovira M Rozman C 1995 Myelomonocytic antigens in B cell chronic lymphocytic leukemia Leuk Res 19 841 848 17 Lohmeyer J Hadam M Ho A Hesse A Pralle H 1987 T cell prolymphocytic leukemia T PLL with unique surface phenotype Blut 54 223 229 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC BECKMAN COULTER 6603266_0911
23. 95 n 54 1 2 90 3 4 5 6 7 WM 2 8
24. ocyte macropfage differentiation antigens on HL60 promyelocytes undergoing difterentiation induced by leukocyte conditioned medium of phorbol diester Leukemia Res 5 491 495 7 Nadler LM Ritz J Griffin JD Todd RF Reinherz EL Schlossman SF 1981 Diagnosis and treatment of human leukemias and Ilymphomas utilizing monoclonal antibodies In Progress in Hematology XIll E B Brown ed New York Grune and Stratton 187 225 8 Foon KA Todd RF 1986 Immunologic classification of leukemia and Ilymphoma Blood 68 1 31 9 Foon KA Gale RF Todd RF 1986 Recent advance in the immunologic classification of leukemia Sem Hematol 23 257 283 10 Griffin JD Davis R Nelson DA Davey FR Mayer RJ Schiffer C Mclntyre OR Bloomfield CD 1986 Use of surface marker analysis to predict outcome of adult acute myeloblastic leukemia Blood 68 1232 1241 474 11 Drexler HG Minowada J 1986 The use of monoclonal antibodies for the identification and classification of acute myeloid leukemias Leukemia Res 10 279 290 12 Drexler HG Menon M Gaedicke G Minowada J 1986 Reactivity patterns of monoclonal antibodies positive on myeloblastic leukemia cells sa defined by esterase isoenzyme analysis Cancer Immunol Immunother 21 188 192 13 Buckley PJ 1991 Phenotypic subpopulations of macrophages and dendrftic cells in human spleen Scannind Microsc 5 147 158 14 Goyet SM Ferrero E Rettig WJ Yenamandra AK Obata F Le Beau MM 19
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