“WAKO BIO WINDOW” No.13(1998. 8)
Contents
1. 0 0 0 0000 0 0000 0 0000 30700000000 L 0000 0000000000000000 300000 0000 0000 0000 EDITADO DDD DD DO DO 0000 0 0 DNAD L 000000000 0 000 0 0 00000000000 0000 0 gt 000 DNAJ O 00 00000 M 1 2 0000100000000 nnnnpNAnnnnn 1 0 0 0000000000 00000 kb 00000000000000 DNAD 0 0 10 0000000 23 1 000000 94 ee 0000000 23 1ko0 O00 5u m 104 0 0 DNAH O D 00000000030000 0000 000000000DNAg DD Globin D 0 262bp0 0 0 O00 Ss 1000 000 2 0 Hd EL E L U M o X174 Haell I J I I O00 DNA np 5 000000 DNA np 100 000000 DNAD nga Kit DNA A WB Rapid 1 WB Rapid 4 92 Un api ug 1 27u g OOOD WeRapd 2300 oe OOOO 10 EDTAJ DD 4 100000000 AL 0 264 g 000020000000049 10000000 DD No o o 00000000 297 54801 m 200 9500 DNA Extractor WB Rapid Kit 293 54803 2000 0 20 000 000000 Lu W Hirayasu K Ishizawa M and Kobayashi Y Nucleic Acids Res 22 17741 19041 Wako 62. LU 2 000000000 l SAITIHHEHUHEBEHEHHHRRLUHL 3841000 153870 0 0 0 0 0 0000 2 00000000 000000000 2 0000 0 00000000 00000000 00 0000000000000000 0000000 0 2 angan A 0030 8400000000000 No BERE HERB iu 539 67021 F 039 200 0000000000 1 533 51431 F 039 038 00000000 1 530 51441 F 039 046 0000000000 DR 537 51451 F 039 034 0000000000000 1 534 51461 F 039 044 000000000 1 532 61271 SH015 683 00000020 Wing 10 533 51811 5 015 686 0000000000000 Mag 10 530 51821 SH015 695 0000000 X Readil Wing 10 534 52061 F 129 003 00000000 1 536 67031 F 129 005 00000000000 1 WAKO BIO WINDOW NO 13 AUG 1998 Frases sf ovens oon pos porco ocon 0 0 00 00 0 0000 Hl UU DI uuu TECAN 0000060 360 700nm lt 3pg fluorescein well oEULULLU 2301 750nm 00 00000 00 00000 0 0 0000000 00000 0341000000060 00000000000
3. HIRON 000 TECAN 20000009 Genzyme Q amp A 0000 PDIFCO I DU DU Precworoses O O O ni ISOPLANTO DD m H DNA 1 EL U ISOPLANT phenol chloroform yield u 9 9 A 260 280 yield u 9 911 A 260 280 Anabaena variabilis 125 500 1 8 60 500 1 7 Bacillus amyloliquefaciens 250 500 1 8 1 000 1 8 Bacillus subtillis 100 1 8 Escherichia coli 125 500 1 8 250 1 8 Haemophilus influenzae 250 1 000 1 8 250 1 7 Pseudomonas aeruginosa 80 125 1 8 m Staphylococcus aureus 125 1 9 125 1 7 Thermus aquaticus 500 1 8 500 1 7 Xanthomonas holcicola 100 1 8 500 1 7 H DNA 10 D L1 U yield u g mg A 260 280 Saccharomyces cerevisiae 0 504 1 83 Shizosaccharomyces pombe 0 348 1 79 Phichia pastoris 2 523 1 87 lane 1 Saccharomyces cerevisiae 2 Shizosaccharomyces pombe 3 Phichia pastoris M Marker 6 A Siyl digest H DNA D I yield u g g A260280 yield u g g A zeoyza0 Mycobacterium phlei 10 8 1 79 Rhizopus nigricans 23 0 2 04 Nocardia asteroides 21 2 1 92 Penicillium chrysogenum Q176 32 8 1 80 Nocardia corallina 125 0 1 80 Aspergillus awamori 36 1 1 62 Streptomyces albus 3 3 2 22 Aspergillus japonicus 20 5 1 90 Streptomyces phaeochromogenes 500 0 1 70 Aspergillus niger 36 0 1 73 Streptomyces sp 2 1 0 80 2 00 Aspergillus oryzae 6 8 2 04 Streptomyces sp MY 31 8 00 2 09 Neurospora sitophila 34 8 1 73 123 45 67 8 9 10111213 M lane 1 Mycobacterium phlei 2 S
4. 0 0 0 0000000000 0000 0000000000000000000000 0 0 0 0000000 0 0 0 000000 000 gt O000 0000000 0000000000000 0 0000000 0000 LAL 90 0 0000000 Limulus polyphemus TO 000000000 00000000008 4300000000000 2010000 0 3m 000000000 00000000000 2010000 0 77m1 DOBO DO DD OD DO ODD lt LALO II O 000 0 0 0 mu 0000000000000 2000 0 00 0 0 0 Umi 2 0 0000 0 0000 0000 2 0000000 D 0 0 0 DU 10m 0000000 000000000000000000 201 000000 100000000 0 TUUUIDDUUUUUIUIUUUUUUUIDDUUUUUI TUUUDUUUUUUUIUUUUUUUUUUIDUUUUU pUUugaddumimpBulututuliuiutibililitutliulutitLitLLl O TUUUDUUUUUUUIUUUUUUUUUIDIDUUUUU 00000000001 000000000000000 T drett 0000 0 0 0 0 00000 0 0 pH3 8 H s 01 00000 031mgQ E coli 4 9 000000000 UU l H EHH Kd 73x 105M E coli UKT B LPS O 409 S om TIL ees LAL 00000006 une A O 00000000000 of S 3 lt 00000 No 00 UO 00 0 00 299 54501 Limulus PS Single T est wako 2011 98 000 000000 111 MinobeS NawataM VVatanabe T and T osa T Anal Biochem 198 29211 199111 2100 00000 dodo 0 00000 00000 0000 00000000 43 1365 1993
5. 0 UU DOBO DO O DD OD DD OD 0000000000 HUO UTU OD O DD OO 000000000000 DOBO DO DO DO DD OOO Doo 00000000 DO DO DO 0D OD OO DDD ODO DAD Doo 00 H A oooO ODO DD OOO Doo E DOB O oO ODO DD ODO DD 0000000000 lab LLL dob Lulli xw 4 HUA HOHA DO DD DO DD 00D 00000000000 DODO DO DDD OO DD OD 00000000000 DODO DO DD 00D DD OD 00000000000 DODO DO DDD OD DO NA DD 00 0 000000 0 0 000000 0000000000000000 00 00 0 0 0 000000 0 0 0 000000 0 000000 HOH QUU DDD OO DD OD 00000000000 000000000 000000000007 0000000000 UUguutlt Wako 0000000000 00 0000000 0000000 O O O No 036 17151 Chromato Sheet 20x 20cm 251 10 000 22 WAKO BIO WINDOW NO 13 AUG 1998 Donn n 1 00000000 000000 00000000 00000000 0000 FAX E mai 00000000 00 0 00 0 ab cd O O Oron ip 400 000 00 000 FAX 5000 0 0 0000000 0 0 30001 LI O a 7 No20 000 000000 diga UOUUUUUUUUUUUUUUUUUUUUOO dIRHEHHHH 000 18100000000000000 0 00 0 10 0000008 260 00000000000
6. BIO WINDOW NO 13 AUG 1998 nun Tecan Jo0000009 Genzyme Q amp A nnnn Porco 0000 HIRON ECHNOLOGIES 0000 m DOOOODNADODO f Drop PCR Human Blood Drop PCR Kit for Human Blood O O PCR 0 600 0 0 000000000000 00 0 0 0 0 0 0 0 0 600p 0 0 Drop B 600p Gene Tag 5units u 150units HEHHHHHHH PCRHHHHHHHUHHH dNTP Mixturell 25mm escht 240u 1 HOUOUUUPNAUUUUUUUUUUUUO Control Primer Du 7 DNAH HHHHHHHHHHHHHHHHHLH forward and reverse 20pmol u 7 each 0000000000000000000000 ceding Buffer b SEN 0000000000 0 0 0 0 0 0 0 0 0 0200 0 000000000000000000000 YUU 2 000000000000 00 OO PCR I 0 000 0 0 0 Gene Tag dNTP Mixture 000000 40 000 20 C 80 01 0 000 JUDDDDUDDDUDDDUDDDUDUDDUDDUD 200000000000000000 0000 0000000000000000000000 0 0000000 0 01 60000000000000 Gene Tag O00 p53 Primer Exon 6 No 319035211 0000000000 00 0 00 0 00 2530 00000000000000 0 0 0000 nnnnnnnnnnnnnrn 0000 00000 EDTA 0000 00000 00 0 0 0 0 0 O PCERE M 1 2 3 4 1 2 3 4 1 2 3 A M UUUOUUUOU 0000000000000 000 0 0 0000 0000 1kbp 0 0 DNA 00000000 0 D 0000000000 0000000000 00 0000000000000 00 000 0000 000000000 00000
7. 000 DO FOOD DO DD ODO DO DO Doo Do d440d00 00000000 18D 000 0 1 0 0 1 0 000 000 0 000 UHHHHHHHEHEHLHHLHLUTBEDVU L TT T ETELHLEHEHLHELULULUL DADO DO DO DD ODO DO DODODOSD 0000000000000 098 0 0 000 DODODODODO TUNELOODODODO DODO DO DO DD ODO DO DO DODOL 00000000 1040000 0 BD000 00 DADO DO DO DD ODO DO DODODOLD DODO DO DO DD ODO DO B Bd 000000000000 0 00 0 000 00 0 0 0 000 UU 0000000 295 53501 Apoptosis in situ Detection Kit wako 400 60 000 0 00 0 0 0 0 WAKO BIO WINDOW 13 AUG 1998 00000004 Geneya 0000 porrco 0000 ratos 0000 jk 300 0 0 0 0 DNAUU UU UU 4 DU 00 UU DNA 10180000 0 w 6 90 00 DUL 0000 0000
8. 18 WAKO BIO WINDOW 13 AUG 1998 De Tecan mm 0 E o DOJINDO AR l BLDVPIPGRFDRRVSVAAC Ad 5000 OOF DR l D LDVPLPAKADRRVSVAAC DACH Og DU Single Letter Code for Amino Acids Alanine Phenylalanine Lysine Proline Threonine Cysteine Glycine Leucine 2Glutamine 2Valine Aspartic acid Histidine Methionine Arginine Tryptophane 2Glutamic acid 2iso Leucine Asparagine Serine Tyrosine 0 0 DE 0 0 0 0 D L 000000000000000000 00 Fluorescent Resonance ee d Energy Transfer 0 000000000000000000 000 00 ei UUDUDUUUDUUUDUUUDUUUUDUDUUUDDUUOUDUUU CH Acrylodan IJUUUUDUUDDDDUDUDUUUUUDDDUUUUUUDUDDUDUUU 5 UB AR 0000 0000 0 0 0 0 0 0 000 000 0000 0200 TA DOdOoD ODO doo PKRAgOo o 0 000 0 0 00 O regulatory domain CA II Acrylodang 0 0 0 pacmppago00000000 HsC N CH DACM 0000 DHUUUUUUUUUUUUDUDUDUDUIUDUDUUUUDUDDDDUDDDUDUUUUUUUUUUUUUUDUDUUI DODODO O 000000050 0 0000 00000 Ii A ex 366 nm A em 524 nmi DR I A ex 386 nm A em 475 nmi 0 ARII J OOO NG108 15 AR II 000000 Forsking D D 0 D D B 00000 DRINOODO 00000000000 DDR1 00000 L gl tamatell OOOO PKAD0000000000000000 00000 000000 00000 00 0000 00000 0 H ul ulu
9. OD DO DD OD D0D0D00 copopBoc 30m LU UODEULLU 00 0 0 00000 0 0 0000 ee ey OTA TH LU DL OL EE UE D 00 020 0 000000 00000000 4 nooog n SE 000100 0 OO 50 x2 100 x2 10000073 0000 o 00 5 2 000000370000 e doe 0 7M0 0000000 50M 00 uuuguli 379 200 0 0 05mM 0 1M 0000000 20m 2 0 DDD HH SE e 000000 ABCO D 00000 0 370 2000 DD 50 x3 0000 100 00000 00 00 300 0 100 0000 0 12 24 36 e lt lt lt 0000000 0146 O00 O 10 5 2 5 1 25 0 625 0 313 0 156 0 078pg 0000000 0 0196 0 O D 0000 160 0 HepG2 00000000 Caspase 3 Q 0 D 0 0 00 0 20 0000 0 20 O O No A OP OPO 0 Ja A BE LO 291 54603 ImmunoStar Kit for Mouse 25 000 297 54703 ImmunoStar Kit for Rabbit 1 000cm 25 000 E VAKO BIO WINDOW NO 13 AUG 1998 13 orco o on o fritos 0000 ik gi 10000000000 0000 Dr Western Gj AULT MLR Rit O DD OD DO DO 000000060 001 002 0000000000159600000000000000000 0 0 Sit 55k 42k 001 OO Drwestem 2u 0 000000000 0 05u gr 0 00 0 HHHHTHTT hRALT 0 00 0 14 9 0 00 0000 0 0 000 0 0 0 00 10g o ml d k 4 000000000000000000000146 1 3 00
10. 0 0 0000 0 0 020 0 0 0002 0 0 00 0 0 020 0 0 t 000 0 0000 0 020 Odo BDO DO DO ODO 00 0 00 Ll 0 000 0 0 0 0 0 000 000 0 020 000 endonuclease VO LJ U J J 20000000 ladder formation 0 0 E H E H E E E C LI DAOBB BO DO DO OO DO DD 000000 TUNEL Gavrieli 19920 0000000 00 DNA 3 OHTE 00 0000000 dUTP deoxyuridine triphosphate T dT deoxynu cleotidyl transferase JOU E ED E E E E E E D LH 00 00000000 00 0 0 0 0 00 00000000000 00 0 0 0 bursa of Fabricius BB U 000000 uuuumuuuuusuuuuuubuduldtutltL t TU AHH 100000000 000000000000 00 0 0 0 0 0 DO DODD 000 000 1 0 0 0
11. 00000 00 00 0 00000 UU 0000 5408605 000000000312 00 00000000 UU 00 00000 000000000000 00 00 000 UU 00 0 0 00000000 02 00 00 0 00 UU 00 00000 06 201 5965 L 0 00000 HL UU HHUHLUL E mail biowin wako chem co jp 00000000 ENE M R H C 0 0000000000000000000 0000000000000 UUHHHTLOHHLRELU U 20000000000000000000 00 9000 4 000000000000000000000 0000 U 5 00000000000 OOOO0O0000000000000 0000 0 00 0 9100 00 00 nge ee ngu BET NANDA DEN 000 01 00000000000000000000000 0000000 0000 nooo 000000000000 090000000000000000000 00 OOUI UU ppp 0000 0000000000000 DO 00d 20000000 BO O0000000000000000000 000000 0000000000000 040 900 D DLL MOO0000000000000000000 UU ONUHHEHEHEREERRLU 090 00 0000 0 0000 00 OO 00 0000 0 0 00 oog 0 OO000000000000000000 0000000000000000 0000 00000000 0 200 agnmggagggaggaggaggagaggadgug 00000000 0020 220 00000000000000000000 00000 0 200 0000000000000000000 290000000000000000000000 000001000 00 0 00 290000000000000000000000 ULUHHHHHLEILEEHHHHHL LLH O 001 00000000 80 2411 8 28 00000000000000 000000000000000 9 2111 90 23 00000000 0000000 9 2811 91 30 00000000 00000 9 300 100 2 0000000 00000000 101
12. 0000000000000000000 o 1438001 28 2019 41 603 55 004 68 4060 81 807 Q 000000000000000000 d 00000 d 0000000 O 000000000000000000000000 Q 0000 00000000000000000000 A 0000000000010000 e A 000000000000000000000000 CBBOOO00 2000 4 DD 5 22 00000 1001 2009 0 F e O O O fe 145 v0VX XkX WAKO BIO WINDOW NO 13 AUG 1998 pj EE 000000000000 Ml uuu onmo mar gt 0000000000000 DO IgG nn d d IgG2 00 o d 00000000 1M00000000010200 gG A 000000000000000010000000 lgGa 00 2 ED 000000000000000000000000 m 1086200 0 gt 00000000000000020000000 2 Q Q Be 00 IgGzb 1 x Q Fa q 2 _ d Q 0000000000 G ot 3 9 000 Fe 0 2 A 0000000 Prtenagooordooodog IgGza 2 1 1 IgGzb S 1 Q Q IgGzc Q O uonuoannonuonnonnonn 00 IgG d 5 UU 1962 e 5 Q Q 0000 or Q b 000000000000000000 Tn me Bs j 0000000000000000000000000 00 d SOOOOOOOOOOOO m p1869 0 gG v pr 19970 00000 IgG 00 s Lou we dH ooo mu un 0000000000 H E d 25 10 H j 1 A 000000000000000000000000 00000000000 000000 o 0000000 ee OD J Ez A 00000 90 0 DL BIOS A H S Bk XS 005000000000000 0 081055500 0 UD UD U D UU 7 00 000 000
13. 111 Adams S R Harootuinian A T Buechler Taylor S S URL http Awww dojindo co jp Tsien R Y Nature 349 6941 199111 E mail info dojindo co jp 211 Higashi H Sato K Ohtake A Omori A Y oshida S Kudo Y gb E 0120 021557 FEBS Lett 414 531 19971 HEHHHLU 0120 489548 VAKO BIO WINDOW NO 13 AUG 1998 19 00000 TECAN 00000004 Genzyme osa anon orco nano fos 0000 ah mi 00000000000000 00000000 SynPhase D 0 00 HES HUHUHHHLHLHLLE 00000000000 0000 000 B H m m Mies 50 TFA DCM 95 TFA H20 COOH OH Strong base 176TFA DCM Amines L TFA DCM nr 4 COOH Base HO SAF Secondary amide forming linker TRH Tritylalcohol linker Hyperlabile linker 000 No II UU U L ooooooooooo 1 HE 000000000 539 68981 SP PS O SAF SynPhase PS Secondary amide forming linker Standard 1001 90 000 532 68971 SP PS I SAF SynPhase PS Secondary amide forming linker Macroll 1001 160 000 533 69001 SP PS O TRH SynPhase PS T ritylalcohol linker Standard 10011 59 500 530 69011 SP PS I T RH SynPhase PS T ritylalcohol linker Macro 100 99 000 537 69021 SP PS O HYP SynPhase PS Hyperlabile linker Standard 100 59 500 534 69031 SP PS HY P SynPhase PS Hyperlabile linker Macro 10011 99 000 53160041 SP MD O HYP SynPhase MD Hyperlabile linker Standard 100 59500 538
14. 20 1 8 rice 10 20 1 8 10 20 1 7 x cetyltrimethylammonium bromide D DNA II II UO D U DNAH 020 2000000 coR DD Hindli 00000000000000 DNA PCR E tobacco ribulose 1 5 diphosphate carboxylase large subunit gene 000000000 00 0 0 0 0 0 DODO DD DDD OO 000000000 M 1 2 3 45 lane 1 spinach 4 tulip o OO a a a a a a a a a a a a a ORO a a a a a a a a a a a a a a a a a a a a a a a a a lane 1 spinach a intact 2 Arabidopsis thaliana EcoRI digest 2 Arabidopsis thaliana 5 rice 3 tobacco c Hindlll digest 3 tobacco 4 rice Marker 6 A Sty digest Marker B A Styl digest 2 O D DNA 7777777777 7777777777 D DNA II II II ILU DNAH 020 2000000 EcoRI DD Hindli 00000000000000 0 DNA PCR 0 0 0 00 00000000000000000 M 1 2 3 4 lane 1 Escherichia coli M109 all intact lane 1 Escherichia coli M109 3 Bacillus subtilis 2 Staphylococcus aureus 3A b EcoRI digest 2 Bacillus amyloliquefaciens 4 Staphylococcus aureus 3A 3 Pseudomonas aeruginosa c Hindll digest Marker B A Siyl digest X22242 OOO pi 2 q a LLL LLL LOL OLLI CR COSE LLL LL LOO LIENS OO OOO kT OOO OOO OOO OO OO OOO OOO OOO a a a a a s MH Marker 6 A Sty digest 10 WAKO BIO WINDOW NO 13 AUG 1998
15. 80520301 CD40 Biotin Y 000000 1001111 1m 44 000 533 70091 80 5197 01 CD119 Bop IFN y R a chain OO O0000 10011 Im 44000 531 70151 80521101 Isotype Control Mouse IgG FITC 100 7 1m 23 000 538 70161 80521201 Isotype Control Mouse IgG Biotin 100 1m 23 000 535 70171 80 5214 01 Isotype Control Mouse IgG2a FITC 100111 1m 23 000 532 70181 80521501 Isotype Control Mouse IgGz2a Biotin 100 1m 23 000 538 70041 80 5182 01 Streptavidin FITC 1001111 1m 22 000 535 70051 80 5183 01 Streptavidin PE 1001111 1m 29 000 IFN y RB chainll TH HL DD OLD D 00 0 D D D HHEHHHHHHHHH Q H HBEM Ss 000000 80 5192 01 IFN y R B chain FITC 80 5190 01 IFN y R B chain FITC 80 5200 01 CD116 FITCH GM CSF R a chain 80 5191 01 IFN y R B chain Biotin 80 5201 01 116 Biotin GM CSF R a chain 80 5282 01 TNF RI p55 Biotin 80 5198 01 CD120b FITC TNF RIl p80 80 5284 01 TNF RII p75 Biotin 80 5199 01 CD120b Biotin TNF RII p80 LL EL EL E EL E EF 200 80 5204 01 CD54 ICAM 1 80 5219 01 Isotype Control Hamster Biotin 80 5205 01 CD54 Biotin ICAM 1 80 5217 01 Isotype Control Mouse IgG FITC 80 5206 01 CD62E FITC ELAM 1 80 5218 01 Isotype Control Mouse IgG Biotin 80 5207 01 CD62E Biotin ELAM 1 80 5221 01 Isotype Control Rat 1962 FITC 80 5208 01 CD140b FITC PDGF R B Subu
16. Rat Monoclonal Antibody RP1050 OOO BH HL HL D D 0880000000 10kba00000000000008B0000 0 0 0 000000 RP1050 0 0 00 CH120 g 0 FACSQ J 0 0 0 8 200 0 0 0 00 0 0000000 00 0 Nor RP 14 000 0 L Rat 19624 0 0 000000 0 0 00000008000000000 RP1050 0000000 000000000000 111 5u g test 00000 101 30u g sample 000000 111 MiyakeK Y amashita Y Hitoshi Y T akatsu K and KimotoM J Exp Med 180 1211 1994 21 MiyakeK Y amashita Y OgataM Sudo T and KimotoM J Immunol 154 3333 19991 3 Yamashita Y MiyakeK MiuraY Kaneko Y Y agitaH Suda NagataS Nomura Sakaguchi N and Kimoto M J Exp Med 184 118 1998 6 WAKO BIO WINDOW NO 13 AUG 1998 pj 000000 TECAN nir seref 000 0 porco loss cke 000 UUUUDUUDUUUU Apoptosis in situ Detection Kit wako SF ANSCIFANNCIFANNCAFANNCAVANNCAVANNCAVANNCZFANNCAFANNCAFANNCAFZANNCAFANNCAFANNC FANNCAFANNCA INSTI ANNCAFANNCAFANNCAFANNCAFANNCAFANNCAFANNCAZANNCFANNCAFANNCFANNCZFANNCAFANNCAFANNCAFANNCAFANNCAZANNCZFANNCZZANNCZZANNCZFANNCAFANNCAFANNCAFANNCAFANNCAFANNCAFANNCAFANNC ANN 5 A al cul i 26 t es M a ioe zz tm ws 0017 IBV 000000000000 F0000000 00000000000000000
17. UU 00000000000 255 0000000000 LAS inear Alkylbenzene Sulfonate m HOUNo 0000000 0 0 0 L Fi ma 300 06051 906609 LAS ELISA O 0000000001000 M00 50 000 307 06061 910156 LAS ELISA 000 0 D 0 U O WOO 200 0 U 35 000 000000 100000004900000000000000 pp494 495 1998 31 Fujita er al Environ Sci Technol 32 11431 1998 214000000 48100000000 00 0 00 0 pp484485 19971 2 00 0000 1214 Er CL 000 010 000 EIE HL RTI EIL EI ipu 4 una Jah 8 TELLE EA EI 0101010100100 E LETTER ETE TET IET ALYA T SEARING ap H 5540 86050 0000000000010 20 060 203 3740 0006 O 6103 00230 00 0000000000050 130 28 03270 8578 0 0 0 e 0000 092622 1005 6 ef G00 0 082 285 6381 9 e 00000 0520772 07880 6 eg 0 0 uu 045 476 2061 9 ej 0000 0487641 1271 0 e H 0 O WH 0299 58 22761 e 0000 0220222 30727 9 e 0 0 01171 02850 poo000000120 052 09 0000000 0 0 0120 052 8066
18. 0 11112 O O DrWestem 1u H 000000000 0 025u g O Rabbit gG whole molecule 10u 9 0 O O Rabbit IgG whole molecule 1u 9 0 HU 000000000 1460 HI LU DTODDDOND 15k PVDFT Tu TL 00000000 0000 00000000000 m Immuno Star Kit for Rabbit JO 00010000 No ES TR PEEL E A RAI 308 51661 Dr Westerfi EL E UE E E E E E LH 40u 1x 5 20 000 Dr Western 0 RepligenQOOOOO0OOO00000000000000 SHSS SS SS SS SHS SS SS SHSH SS SHS SH SH 0000000000 0000000000 099 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 99 099 099 99 99009 e O e 0 00000000000000 1000000000000000000 5 Aa 0000000000000000000000 5 0000000 21 II Total 0 3u gili CBB O ODO 104 00000002 0000000000 2 7 Q Q 0 0 Q Q 0 0 0 e e Q 0000 0000000000 0000000 9 0 0000 00 0000 5 00000 y 0 FA 00000 304 g 200H 1 150ng u IT 2u 10000000 Total 2 00000 0 0 34 00 000 00 00000 Dr Western A A ooooo0 SDS PAGE 0000000000 Q g 000000 2 000000 505 000000005090000000 Buffet 0 000000 A A 900 2 SDS 5 glycerol 0 005 BPB 62 5mM 7 1 Dr Westem 0 0000000000000 gt Tris HCI pH 6 8 E R Proen af 0 DD DD Dat m e D DDD 0 0000000000000 d s 00006 00000000000000000 2 g d 0 2 ika 4 002000000 2020010000000 2 00000000 000 0000 NAN 600000000000000000
19. 69051 SP MD I HYP SynPhase MD Hyperlabile linker Macroll 1001 99 000 535 69061 SP MD O T RH SynPhase M D T ritylalcohol linker Standard 100 59 500 532 69071 SP MD I TRH SynPhase M D T ritylalcohol linker Macrol1 10011 99 000 000000 SAF Constantine G et al J Org Chem 62 1240 1997 TRH Takahashi T et al Tetrahedron Lett 39 1369 1998 DO DO DD O DO OD O DO 00 000 00 00 0 0 0000 0 0 0 00 0120052099 0000000 L 0120052808 20 WAKO BIO WINDOW NO 13 AUG 1998 Ll 0000 lJIUUDDUDUDUUDUDDUDUDDDUDUDUDUDUUDUDUDUDUDUDUDUDUDUDUDUUDUDUDUDUDUDUUDUDUU UHED UHHEHHHHUHEHHHHHEHHEEHHEHHEHEHEHHELHLHLLULLL J okt 0 uuuuudgdgaauduuuuuluuluuuluuliiuililililLiliiLibiiliiiilii B iiutitittttLl DO ADD OD BO DO O DO DD O DO DD OD ODO DD ODO 0DO0D0L OD O A O O O O O O BB NDULU DUDU NU DDD iutiuttLutlutlulu lIUDDIDUDDUDUDUDUDUDUDUDUDUDUDUDUDUDDUDUDUDUDUDUDUDDUUDUDUDUDUDUDUUUDUU 000000000 0 1006 0 0 0 0 0 0 TT 500mmol KC H 2mmol DTT 1mmol 7 EDTAT Immol 7 OO OOO L 0 O 0 O 50mmol Tris HCI pH 751 80 O SDS PAGE 00000000000 O No L L L 0 Fih ula ee DAH 541 00621 Estrogen Receptor recombinant Solution 750pmol 44 000 000000 111 Obourn J D er al Biochemistry 32 6229 1993 3 Tzukerman et a
20. ODO DO OO O DO ODO DO OOO DO OD OO Dd Doo doo o00o 00000000 0 Difeod 0d Oo do gogo do go ga a Do do dogo ooo 00000 AAA AA AA AA AAA AAA AAA LLL Difco UUDUUDUUDUDDUDUDUDUDDUDUUUUUDUUDUUDUDUDUDDUDDUDUUDUUUU uuddulult Difco Product Categories Culture Media and Ingredients Dehydrated Rapid Testing Culture Media P repared Reagents Buffers and Enzymes Custom Media Development Serology and Immunology Foodborne Pathogen Testing Specimen Collection and Transport Laboratory Products Tissue Culture and Cytogenetics Manual Blood and Accessories Difco Main Products L L L L L L Agar Bacto Casamino Acids Technical Proteose Peptone No 3 Agar Granulated Casein Digest Soytone Agar Noble Casitone TC Lactalbumin Hydrolysate Agar Technical Neopeptone TC Yeastolate Beef Extract Bacto Peptone Tryptone Beef Extract Dessicated Proteose Peptone Tryptose Casamino Acids Proteose Peptone No 2 Yeast Extract DODODODO 000 0000 BB DD DDD OO DD DDD O DD DD 000000000000 SPSS SS SHS SH SS SS 0000000000 SH SH SH SH SH SHSHS SH SH SS SH 0000000000 99 99 99 99 99 99 99 99 69 949 99 69 949 99 99 99 99 99 99 SHS 99 99 99 99 99 99 SH 99 99 099 99 99 elil 311 00 0 100 90 11 00 0 PM13 200 180 00 000000000 0000000000 2 4 20 0000000 ELA D Aa Apan DEE Ed HG n n 0 5 xXDO0pg008080
21. 0 0 00 00 0 00000000 UU UU UU 000000 340000000 S3 00000000 0000 0000000 000000000 00000000000 e e e e e 00000000 e e e e e e e HOUUUUUUUUUUUO MUUUUUUUUO 00000000000000 0 0000000 0000 100 030 00000 200200000000000 0 0 00 3401 750mm 0000 000 0000000000000 50 200000000000000000 200000000000 2000000 DD 405450 92 620nni d 00000000 0000000000 0000000000000 000000000 DZ dd eee q nnnnnngnn6 WAKO BIO WINDOW 13 AUG 1998 Pa recan if creme osa 1 05 porco on fetal 005 ME 0000000000000 1 Transome 00000 T cell tine 11 0000000000000 Jurkat cell line LL D D B 000 D D U D 0 D stable expression O0 00000000000 Wild type Jurkat Hi 200 30000000000000000 0 0 0 0 Stable transfectant 111 0 00000 00 00000 00000000 pZeosv2 Invitrogen 0000000000 29 g H U D 1x 10 150 H UU T ransome O 00 0 200 0000 0 0 0 0 OPTI MEMQ GibcoBRLO 0 0000 0 0 L 002000000000000000000 smi Complete medium 116401 101 FCS 000 3000 0 000 1004 gh m Zeocinil DU Complete medium smi O O 000 0 0 30 0 0 0 0 0 0 30 0000 2004 0 0 m Ze 0 O0 Complete medium O D U D U L 000000000000000
22. 00 000000000000 0000000 000000000000 02 n TAA EN n a ee LU TET TEST KA Vu Uu mHn ununu nn nn III Rh RR RR RR D S TAA JA RR RR RR RR RR PA LA id naa ELE 4 2 1 iH il 2 1 19 12 iz 3 lili 4 2 il mamana 000000 1000 0 00 LI 0000 n ih AA 0 HO 1001 HHDHHHHHBHBHHEHHHHEBHHSEB 00000000000000000000000 0 00 0 0 0 00 000 HE Be ll lu E 2 hu LEE EE Bt 0004008 TEL 0298 58 2278 0298 52 6051 E mail nakazato hideo wako chem co jp 6 WAKO BIO WINDOW NO 13 AUG 1998 n ATPU D D LI L if SOY ATPIHIHUHSEHEHHEHHUHHEHUHHHUHHEHUHEHUHEHULHLHUL 0 0000 000000000000 10 mol 10 mog J 0 0 00 0 0 0 00 0 00 0 000000 00004 0100 470000000000 00 0 0 00000 D ATP ep 000000 O 0 0 0 0 0 0 O 0 0 J 000 021 T 560nm Mo 0000 00 UHHHHHUHHHEHHHHUHHUL 0 0 0 0 0 0000 DU 000000 LIDODO0 DO DO DDD 00000 000
23. 000 00000000 0 0 0000 0000000000 00 00000000 000 0000 000 0000 00000 000000000 00 00 00 0 000 M Marker5 p X174 Hinc digest 000 0 20C PCROOO 2 500 104 0 0 0 0 DL No 318 03851 Drop PCR Kit for Human Blood 600 55 000 Drop PCR Kit for Human Blood HN Hu 0 DO 0 B B D B B 0 000 0 0 00 0 00 T his product is sold under licensing arrangements with F Hoffmann La Roche Ltd Roche Molecular Systems Inc and T he Perkin Elmer Corporation cap Site cDNA L 0 B E E E J C p0 4000000000000 99000 547 315 03741 Cap Site cCDNA Mouse Embryd 16days 313 03801 Cap Site cCDNA Rat Ovary 312 03751 Cap Site Rat Embryd 18days 310 03811 Cap Site cDNA Rat Pancreas 319 03761 Cap Site cCDNA Rat Kidney 317 03821 Cap Site cCDNA Rat Skeletal Muscle 316 03771 Cap Site Rat Liver 314 03831 Cap Site cDNA Rat Small Intestine 310 03791 Cap Site cCDNA Rat Lung 311 03841 Cap Site cCDNA Rat Stomach 313 03781 Cap Site cDNA Rat Testis m VVAKO BIO VVINDOVV NO 13 AUG 1998 H RUZ Q amp A nnnn P orco uu MO a dg if DO UU UUUUU I D D El ISOPLANTI I 1 1111111111 DNA AMM D DNAT 1000 ISOPLANT yield u old A 260 280 yield u 9 911 A 260 280 Arabidopsis thaliana 100 120 1 8 15 30 1 8 Spinach 80 120 1 8 30 50 1 8 tobacco 411 20 1 8 60 80 1 7 tulip 10 20 1 8 10
24. 0000 000000000000000000 000000000000 00000000000000 00 00000000000000 0000000000000 000000000000000 0000000000000000000000000000000 000000000000000000000000000000000 00000000000000000000000000000000 00000000000000000000000000000000 00000000000000000000000000000 0000000000000000030000 0 0 810520 105201 8105301 00 000000000000000000000 00003000000000000000000000000000 0000000004000003000000000000 0 0 BIOS 0 000 00 0 0 81092000 O wakon 00000 nu 0 0 O URL http www wako chem coj p 0000 0 0 0 0 0 0 00000000000000000000000000000000 50 000001500000000000000000000000000000 06 0000000000 00000000000000 000 E maili 000000000000 000000000000000000000 DO E maig 000 000 00000 540 8650 000000000 312 0000000 00000 00000000000 06 201 5965 E mail biowin wako chem co j p BIOS 10L 2 000000000 000000 000000000 WAKO BIO WINDOW NO 13 AUG 1998 n 1 Tecan 00000004 Genzyme osa onon f orco nono pese unan ah mi 00000000000000 5 0000000000 fug 50 300 0 0 0 U O O 0 Becton Dickinson Company Difco Laboratories 0 E OO DH Difco Laboratories Becton Dickinson Company 0000 00 DD E EO 000000 0000000000000 ODO DD DDD O A ODO DO ODO DDD DO DDD DO OOO ODO DDD A DDDDoDO HOS WHOD
25. 0000000 205 00000 220010000000000 SE T E E Tna m Bee Transome 1 0x 10 2 5x 10 2 0x 10 pg 1 0x 10 1 0x 10 1 0x 10 Total cell counts 0 0 0 0 00 00 0 000 No 202 14091 Transome lm 30 00011 0 000000000000000 0 0 0000 O small unilamellar vesicle SUVI OOOO ODO DDDOOD DO DNA DD OO BO DO O BBD OO DDD OO DD 000000000000 DOABODO DO ODO OD DO DDD DDD Lt 0 0 251 Au 111 E Selective antibodies to beta amyloid D n 1 40 1 42 and 1 43 JUUDUDUDUBUDUDUDUDUDAB MUDUDUUDUUDUDUUDUDUUUDUUUUUUUUUUUUD 000 0000000 0 0 00 534 68551 44 348 Anti Amyloid B Protein 1 40 C terminal Selective Antibody affinity purified rabbit polyclonal 251 g 531 68561 44 344 Anti Amyloid B Protein 1 42 C terminal Selective Antibody affinity purified rabbit polyclonal 25 g 538 68571 44 340 Anti Amyloid B Protein 1 43 C terminal Selective Antibody affinity purified rabbit polyclonal 25u g 535 68581 44 338 Anti Amyloid B Protein N terminal Selective Antibody affinity purified rabbit polyclonal 50u g 531 68583 44 338 Anti Amyloid B Protein N terminal Selective Antibody affinity purified rabbit polyclonal 100u g 535 68601 44 136 Anti Amyloid B Protein 1 40 Antibody rabbit polyclonal IgG preparation 100u g 532 6861
26. 1 20 100 4 0000 WAKO BIO WINDOW 13 AUG 1998 J gt 0 00000 000 000000000 APED D OOC 000000000000000000000 0000000 0000000000000000000000 AER DD D H NP10EO 1mg 0000000000000000000000 2000000 4m 1 gt 00000000003020000000000 ennnnnn n n mi 20 0000000000000000 ennnnnnnnnn Sm 2 0000000000000000000000 ennnnnn 250u1 10 00100000000000000000000 mnnnnnn 5m lj z 000 0 000000000 enaeennnnnnn 0000 0 00 00000000 nnnnnnnnnnnnn o well 11 0000000000000000000000 epnnnnnnnnnn o well 10 000000000000000000000 e innnni 50m 10 02 00000000000000000120000 epnnnn 15m 10 gt 0000000000000000000000 ennonn 22 Eege 77 000000000000000000000 o Q q e 10000000000020000000000 aun 0000000000000000 101010000000000000000000 120000000000000000000 00000000000 0050 05mg HU 00000000 1100000000000000000000 000000000002500060000000 1000000000000000000000 nn Me 10000000 a D n n 1000000000 304 06071 910163 APE EUSAn nmn npppnnnnnl n n 7 0 0 70 000 301 06081 910170 APE EUSA Dm 00000 mmm 20 0 0 50 000 SOOOCOOOL OOO L A G SPOOR OO DIO OKIOKI OKIOKI OOO 0000 0 0 D D 0021 o5mg it 000000000000 0000 0 00 0
27. 1 44 352 Anti Amyloid B Protein 1 16 Antibody Mouse monoclonal 100u g 539 68621 44 356 Anti Amyloid B Protein 17 26 Antibody Mouse monoclonal 100u g 000000000000000000 WAKO BIO WINDOW NO 13 AUG 1998 Fa recan if creme osa 1 05 porco on jon 20 0000000 000000000 yo Wako 000000 DADO BDO DD O DD UU L 000000000000000000000000000000000000000000000 0000000000000000000000000000000000000000000000 000000000000000000000000000 0000 0 sue HHHR 6 DNAD 0 0 0 0 DNAD DUEHRULR 6 24hrs H 000 0 0000000 U 20000000 0 000 0 HE UDUD 3 mmi DA Dd 000000000 0000000 0 00000 0 UHEHHUHHHEHHHHEHERUHHHLRLUHM 0000000000000 0 00 0 0 00 000 000000000 00000000 00 0000 00 000 0 DU 0000000000000 0000 0 00000000000000000 20000000 020000 0 0 0 37 C 020 0 000000000 0200 3 01000000 00000000 000000 00 0000000 H 0 000 0 000 0 DUDUH JUU1IDDUDJDDUUDUDUMLV Multilamellar LL EL EL EE EE E 5 001000 vesicle TI O U T DNA 0 O O No 070 04441 Genetransfer Iyo 0 2m x 5 29 0001 LE EE E
28. E DH 111 Koshizaka T Hayashi Y and Y agi K J Clin Biochem 30000 000 0000 00000000000 Nutr 7 185 1989 4 108391 2100 0 00 Mebio 41 0 O 26 1992 440000 0000 Vol 12 59 1994 000000000000 Wako NMOUUUUU lUUUUO 00000 DB ag nO O O BD DD DDD OOOO OB DD DD 0000000000 400 20 000 00 00 UHEHPBHHHLHLELHABD 142 430000000000000000000000000000 00 0 0000 000000 400 000 4337000000000 LI D PBSO O 0 0 0000 000000 40 0 0 0014000000000 0000000 ELISA 11 5 000 000000 0000000 111 100 0000 1 1 000 No L L L L 2110 Il 13 0100 013 17321 Anti Presenilin N terminus Goat Img 50 000 ads __ wAKO BIO WINDOW NO 13 AUG 1998 joan Anan 00000004 Genzyme nn n forco 0000 gaa 0000 ME 00000000000 nf 0000000000000000 Wako 000000 0 Fat Fa sillilili uHHRUHUHuUH RUL uL Anti Mouse Fas Rabbit Anti Rat Fas Ligand Rabbit 0 HOO 50 J 0 0 0 292 3061 00000 ad Fest p 0000 0 UU 4256111 0000 MAP 000000 0 MAP L 0 0 0 0 0 uU DODODO DO DO dB OD OO ODO O O O DO O DD O DD D00D0D0D00D 00000 0 0 000 Fat 0 00 0 00000 0 0 0000 000 000 1000000000450000000000000 0000000000 Fas ligand 000000000000 LEIT CO EI No 019 16181 Anti Human Fas Rabbit 100p 35 000 B000000000000 Wako 000 LH 014 16251 Anti Mouse RP 105
29. L L 000 DD I ATP Standard Curve 2 0 0000 0000000001 3 0 00 0 0 LLO 0 D Ou 11 prm 107 1035 105 0 102 1022 101 1010 400000000000 ATP Concentratiofl mole 1x 10 mole JATP 00 x 10 M 10u 0 ATPY 00 IO 000000000 LLO O O 100g 0 000200000030000000000 0000000 M 11111 1000 000 0 00000000000434000000000000 pH7Z75 LI 000 UD D BSAU DU U U U UUUUUUL 11 0000 0 20000000 12m 0 0 0 000 O00000 BEL Hu BS ANA 308 05991 OOOATPOOOOD 20 000 0 0000000 4 40020 0 0 0 0 00 ATPU UU UU LU WAKO BIO WINDOW NO 13 AUG 1998 LE 0000000 1 0 000 90000000 Wako 0000000000 il UL A ula hin rn urnu l l n HHHHHHHUBDULHLAL 0 0 0 0 0 0 0 0 00 L Pyrosepill L TT E LEHRERHLELEHEHEHEHHELHHHEHRHLELHELELEHHEHLHEHLELU L 000 0 0 0 0 00 0000
30. WANO DIU WINDOW n dononubo ono 0000 0 040 0400000 Rat ik 00000004 0000 bu 0010 DIFCO TECAN EM Q amp A 0000 A kI TL EXE IL A x iena 0609 AL Transome O P4 000 goooocooono bo P5 0 000 VBTEHRDLUUL 8 N G Drop PCR Kit for Human Blood P9 N G ISOPLANTII 0 t P10 o Rn 0 1 0 000 f annnnnnnnnn P6 Apoptosis in situ Detection Kit wako P7 Genzyme t 00000000000 P12 00 11010000 ImmunoS tar Kit P13 Q amp A Dr Westem P14 0000 BIOS P15 Difco 0000000000 P16 OOO DODOPSIOODODODDO P18 00000 0 00 P17 l linn pKaococooe P19 HEHHH 00000000 SyrPhase ggogog0g0 P20 00000000000 000000 21 00000000 21 4900000 24 00000000000 P2 0000000000000000000 P3 8105001000000000000000000 P15 0 30 Wake U DD 0 0 HO P16 00 1000000000000000000 P22 010000000000000000 P23 Genzyme onno forco nnno esta 0 oo Y 1 00000000000000000 ET 0000000000 0 eil illl 230 700nm z 15pg fluorescein well uiubiull 4001 700nm J 04 10 DEA activity units well Alkaline Phosphatesell el UUUUU 2301 750nm Ugiul ULUHUHLULUUHLUL ADODO DO OO DD OD ODO DD ODO 0D 0D 0000000
31. l Mol Endocrin 8 21 19941 21 Brown M and Sharp PA J Biol Chem 265 11238 1990 411 Beekman er al Mol Endocrin 7 1268 1993 000000 Li O No L L 000000100 545 00641 UDP Glucuronyltransferasel recombinant M icrosome Protein 28 000 546 00431 UDP GlucuronyltransferaseT l recombinant M icrosome 5m l Protein 35 000 00000000 IN DUDUH 0 0 i 00000000000 0 000 00 0 0 0020 E SS am A 2 A d ni 000005 98 0000000000 EEUU am i 0000000 0 0000 000 0 00 000 000000000000000 000 0 0000 HUHUUUUUUUUUUUUUUUUUUO uuuBiuiuluiuulilliiu ulltLutlliitL ll z l 00000000 0000 0 lt M inert No L L L L AAA 025 13541 Bisphenol A Standard 500mg 4 500 146 06791 p n Nonylphenol Standard 500mg O 028 13531 p t Butylphenol Standard 500mg 4 500 d WAKO BIO WINDOW NO 13 AUG 1998 000 00000 3 HUUUUUUUUUUUUUO 00000 00000000 DO00006 00 ul DOBO DO OD DO BID BA NANG NANANG OD U UJUDUUUDUUDUUUDUUDUUDUuUDuUpul 0 LUL Brassica napus LO OOOO ODO DO OD OD 000000000
32. nit 80 5222 01 Isotype Control Rat 19G2p Biotin 80 5209 01 CD140b Biotin PDGF R B Subunit 80 5373 01 Isotype Control Hamster FITC 80 5416 01 CXCR4FITC Fusin 80 5417 01 CXCRA Biotin Fusin Genzyme 1 1 000000000000 U U www genzyme conyresearchproducts 12 Pa rec nif onere onn anon porco o coc 11 110000000000000000000000 El ImmunoStar Kit Wako 00000000 OO dB DO DO DDD OD 00 0 0 02 020 0 00 0222 0 02 002 0022 DO 0020 0 0202 0222 IUDUDUDUDUUDUUOUIUDUDUDUUDUUDUUDUUOUIUDUI Li Lilihiiiiu niu u l nu 0 0 0 0 0004 0040 0 0 0 0 0 0 0 00 000 0000 0000000000 0000 0000000 HUUUUUUUUUUUUUUUUUUUUUN OO OO 000000000000 130m UUUUUUUUUUUI 20000000000 0 0446 H L HUUDUUDUUDUUDUUUDUUUDUUUUD 000 100x 1 3m 000000000 00 0 0000 T 100x 1 3m BHIuiulutuiuuutiuuiiutututututt 200000000 10x 30m 000000 0000 HELL 20x 2x 165m DO DO ODO ODO O 0 0 0000 OUUUDA 70m 2 00 000 ULHHB 70m DO BOO O DO
33. treptomyces albus 3 Nocardia asteroides 4 Rhodococcus rhodochrous 5 Streptomyces sp 2 1 6 Streotomyces sp MY 31 7 Neurospora sitophila 8 Rhizopus nigricans 9 Penicillium chrysogenum Q176 10 Aspergillus awamori 11 Aspergillus japonicus 12 Aspergillus niger 13 Aspergillus oryzae Marker OI A Styl digest O No 314 02731 ISOPLANT 10011 29 00011 A WAKO BIO WINDOW NO 13 AUG 1998 n 09 Tecan ono feezyme osa nnno porco nnno Erato nno UUUUUUUUUU al 0000 gt gt ready to use P gt OOOOOUOFIT 000000000 gt gt 000000 50 000 000000 ETE 000000000000000000000000000 0 ITCI PED 0000000 DO gd DO O ODO Bd DB DLL YU LU LU D LI LLL 4uBBiulutltLtttLL UUUUUUUUUUOU 0000056 Dd 0000 O TH TH2 0 00000002 COON nn J UU nma 532 70061 80518401 CD62L FITO L Selectin T 0000000 1001111 1m 47 000 539 70071 80 5185 01 U CD62L Biotin L SelectinfiTTHTHUHU u 1001111 1m 44 000 536 70081 80519301 O IFN y R B chain Biotin THHHHRH 1001111 1m 44 000 532 70201 80523501 0 0 O0 CD34 FITC EH E HL D E CE 1001111 1m 47 000 539 70211 80523601 CD34 Biotin 0000000 1001111 1m 44 000 537 70131 80520201 0 O0 CD40FITCI 000000 1001111 1m 47 000 534 70141
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