λ polyA RNA-A
Contents
1. DNA Control Template amp Primer Set A TX803 DNA A1000 ADNA A DNA RT PCR Real Time Primer for Apoly A 3786 EASY Dilution 3786 RNA RNA 12. ADNA vitro transcription poly A ft EDK 1 0 kb RNA TaKaRa IntelliGene ADNA A1000 ADNA A DNA RT PCR Real Time Primer for ApolyA 3786 RNA DNA RNA DEPC treated water IntelliGene 100 500 pg RNA
3. v201103Da Woo o S a WCapux TaKaRa R DR
4. A into each RNA sample To utilize this product for self production of DNA microarray immo bilize DNA fragments A 1000 or A DNA A which are produced by using A Control Template amp Primer Set A Cat TX803 on the DNA chips Real time RT PCR Regarding protocol please refer to the product manual supplied with Real Time Primer for A poly A Cat 3786 Dilute this product by Easy Dilution supplied in Cat 3786 as protocol Notice Avoid freeze thaw of this product which are liable to disintegrate Utilize this product according to the Notice for using RNA samples Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Produced by TAKARA BIOTECHNOLOGY DALIAN CO LTD v201103Da A polyA RNA A Code No TX802 Shipping at 80 C Size 10 ul Store at 80 C DEPC treated water 1ml Lot No
5. A polyA RNA A Code No TX802 Size 10 pl Shipping at 80 C Store at 80 C Supplied Reagents DEPC treated water 1ml Lot No Conc 10 ng ul Expiration Date Description A polyAt RNA A is RNA fragments about 1 0 kb attached with poly A which are synthesized by in vitro transcription using A DNAas template These RNA fragments have the sequence complementary with A DNA fragments 1000 or lambda A which are spotted on the products of TaKaRa IntelliGene Series Store this product separately in each small amount after diluting them to the appropriate concentration by DEPC treated water For normalization of DNA microarray for eukaryote this product is uti lized for internal control For Real time RT PCR for eukaryote this product is utilized for external RNA as reference with the use of Real Time Primer for A poly A Cat 3786 Usage Hybridization of DNA microarray Before the fluorescent labeling reaction add the appropriate amonut of A polyAt RNA A into RNA sample Then label the sample and use it as the probe for hybridization Adding the same amount of A poly A RNA A into both reference and test sample will enable to normalize the signals after the labeling reaction Store this product in aliquots after diluting them to the appropriate concentration by DEPC treated water In case of using the TaKaRa IntelliGene for Eukaryote it is recom mended to add 100 500 pg of A poly A RNA
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