QuickGene SP kit RNA tissue
Contents
1. MSDS MSDS http Iifescience fujifilm Com 5 gt 15 28C e LRT2. 4 lt g gt CAS WHT 750 3 p 4 8 000Xg 10 000rpm 1 i WTS NIS
3. 4 p 18 D 10 16 CAS 15 88C LRT
4. CAS WTS 15 30mg p 10 5 15mg D 16 15 30mg e LRT LRT 1 ml 10 2 ME e WRT 175ml gt 9996 e DNase p 4 DNase p 14 lt 1 gt gt 15 30mg
5. 30mg e B0C 80C QuickGene SP kit RNA tissue SP RT total RNA 2 total RNA BalD c 7 MicroSmash MS 100 DNase 260 280 30mg 100 12048g 2 2 30mg 40 50 g 2 2 30mg 15 20ug 2 3 e 8 8 1 LRT 85ml 37C
6. TU ul DNase I 0 os i T6l 1 2 US MM UMS 5 LRT 500 16Al mi 5mm 1 2 7Kunitz units ul DNase I 2 1 25u 35 3 75l QIAGEN DNase DNase 2 RNA RNA N
7. CAS 4 1 1 5mIl LRT 2 ME 10 5 1 6 7 8 2 lt 5 gt p 19 5 15mg total RNA total RNA CAS 3 25 10 1 QuickGene SP kit RNA tissue SP RT
8. 8 000Xg 10 000rpm 1 WTS CAS WHRT 750 8 000Xg 10 000rpm 1 WTS WRT 13 14 lt 9 gt DNase
9. 15 5 15mg LRT LRT 1ml 10 2 ME WRT 175ml gt 9996 e DNase p 4 DNase p 19 lt 1 gt gt 5 15mg 1 5 2mm lt 3 gt LRT 2 ME 500 lt a gt MicroSmash MS 100 3 800rpm 120 300 b gt TissueLyser 30Hz 5 Xx2 4 p 18 4 gt 17 000xg 15 000rpm 3 1 5ml 350 lt 5 gt SRT 175l 15 6 gt gt 9996 175l
10. CAS a CAS CAS 8 2 lt gt p 13 p 19 CAS 1 b CAS CAS 1 CAS 1 1 5ml 350 LRT 2 ME 2 RNase RNase 3 3
11. CAS RNase RNase RNase 1 p 7 30mg A DNase RNase RNase Free DNase DNase RNA RNA RNA 23 24 6 RT PCR RNA 260nm DNA DNase RNA 5 RNA WRT 750 I 3 7 DNA
12. p 4 lt 7 gt CAS Smm 8 000Xg RNA 10 000rpm 1 WTS LRT 2 ME lt 3 gt LRT ME 500
13. WTS 1 5ml WHT CAS CRT 100I 8 000xg 10 000rpm 1 1 5ml total HNA CRT 50 I 4 1 p 27 total RHNA 1 5ml 50C 80C
14. DNase DNase 10 gt DNase p 4 lt 9 1 gt DNase Cat No RQ1 RNase Free DNase Promega M6101 1 20U 40 gl Deoxyribonuclease RT Grade 313 03161 DNase I RNase Free Ambion 40U 40 ul RNase Free DNase Set QIAGEN 79254 3 UU units 40 ul 1 500Kunitz units RNase 550gl DNase DNase 1 TU ul DNase I 20ul 10xReaction Buffer 4ul 16 l 2 2U ul DNase I 0ul 10xReaction Buffer 4ul 16 l 3 7Kunitz units ul DNase I 2 LPbu Buffer RDD 3541 S37BLL QIAGEN DNase DNase
15. p 25 CAS 5 RNA B0C 4 CAS 15 30mg p 10 5 15mg p 16 15 30mg 10mg p 10 LRT 2 ME LRT LRHT1ml 10 I 2 2 ME RNase
16. 37C e 1 p 7 e 1 p 7 CAS e 30mg 10mg e 15 30mg p 10 5 15mg p 16 1 1 BalD c 7
17. No LV1 2 3mg 1 5mm 1 5mm o5mm LV 5 0mg 2 0mm 2 0mm 1 0mm LVS 11 6mg 4 0mm 4 0mm 1 0mm LV4 16 2mg 5 0mm 4 0mm 8 0mm gt LV5 1 7mg 5 0mm 3 5mm 2 5mm LVG 5 6mg 6 0mm 5 0mm 2 5mm LVZ 30 7mg 7 0mm 5 0mm 5mm J Lve 56 7mg 80mm 7 0mm g5mm LV9 850 8mg 0 0mm 14 0mm 8 0mm a LV2 LV7 30mg e CAS e CAS WTS e e LRT LRT RNase gt e RNase
18. 10mg 1 p 7 1 p 6 15 30mg p 10 5 15mg pD 16 1 p 7 CAS BOC B80C LRT
19. DNase lt 9 2 gt DNase HNA DNase CAS 1 40 Mi iM ee 0 5 DNas8e P 100 40 I a De 0 9 10 gt CAS WRT 750 15 30mg p 10 5 15mg 8 000xg 10 000rpm 1 p 16 WTS
20. 1 5 gt SRT 175 15 3 gt lt 4 SRT lt 6 gt SRT 1 1 5 4 gt 99 140 1 CAS
21. RNA e RNase RHNase e 8200C 16 6 QuickGene SP kit RNA tissue SP RT RNase 7 QuickGene SP kit RNA tissue SP RT 5 3G0mg total RNA 1 BalD c 30mg 30mg 30mg 3 p 12 e
22. DNase DNase LRT 8 ME 8 3 gt p 12 18 5mm DNase RNA 5 RNA RNA CRT RNA CRT CAS 8 000Xg
23. gt 999 8 1 p 8 15 28C RNA CRT RNA CRT CAS 8 000Xg 10 000rpm DNase 3 p 4 DNase CAS CAS WTS
24. SRT 1 1 1 gt 1 p 7 lt 6 gt gt 99 175 1 1 p 7 10mg lt gt 1 5 2mm MP
25. DNase lt 10 gt lt 11 gt lt 12 gt lt 9 gt DNase DNase CAS 1 40 5 DNase P 100 40 CAS WRT 750 8 000xg 10 000rpm 1 WTS WHT CAS WRT 750 8 000xg 10 000rpm 1
26. Cat CRT QuickGene SP kit DNA tissue SP DT CRT CRT 2 30mg DNase 30mg DNase DNA aoo 4 QuickGene SP kit DNA whole blood SPDB ES CGE TC 150 a 3 DNA bf QuickGene SP kit RNA tissue SP RT 1 100F RNA EE EP CRT CRT ICON CU a PO iool 1o0 RNA 50 l 50l uckoens SBI RANA oed Il 0 eo 120 1go ao 0 eo ao neo ao RNA HC QuickGene SP kit Plasmid SP PL ls e M 1 3 1 Kb Plus DNA Ladder Invitrogen DNase DNase
27. 1 16 lt 7 gt lt 8 gt lt 9 gt lt 10 gt 11 gt 18 gt CAS 8 000xg 10 000rpm 1 fi WTS WRT 750Zl 8 000xg 10 000rpm 1 WTS DNase 40l 5 WHRT 7504l i f 8B 000xg 10 000rpm 1 WHRT 750 i a 8 000xg 10 000rpm 1 U 1 5ml a CRT 100 2 8B 000xg 10 000rpm 1 total RNA 5 1 5mg 5 gt SRHT 175 15 lt 3 gt lt 4 SRT 6 gt
28. RNase 550 DNase 350 NR 1 5ml 1 5 gt 18 19 1 9
29. LRT 1 ml 10 I 5 2 ME SRT 40mD WRT 75ml 175ml gt 99 ethanol added CRT 100ml CRT DNase DNase D 14 9 gt p 19 9 gt 8 2 QuickGene SP kit RNA tissue SP RT 5 30mg total RNA
30. DNase Cm 196 1 XTAE e HT PCH M 1 3 100bp DNA Ladder Invitrogen DNase DNase DNase Cm 10pg l total RNA cDNA G3PDH 196 1 xXTAE 27 Dy FUJIFILM e 106 8620 26 30 TEL 03 3406 2201 FAX 03 3406 2158 e mail Sginfo fujifilm Co jp URL http lifescience fujifilm Com FPT 608106 FP 1
31. e MicroSmash MS 100 TissueLyser e MicroSmash MS 100 ml Cat No 72693 TissueLyser TreffLab 2 Oml Cat No 96 9329 9 01 e 5mm 17 000x g 15 000rpm DNase RQ1 RNase Free DNase Promega Cat No M6101 Deoxyribonuclease RT Grade Cat No 313 03161 DNase 1 RNase Free Ambion Cat No RNase Free DNase Set QIAGEN Cat No 79254 4 LRT Lysis Buffer e
32. e e SHT Solubilization Buffer e e WRT Wash Buffer e e CHT Elution Buffer e e LRT LRT
33. 36 ly WHT WRT gt 9996 12 gt CAS CRT 100I 8 1 p 8 8 000xg 10 000rpm CAS 1 1 5ml a EA Cd ats A a ad total RNA DNase 5 SM 5 WRT CRT I 50 4 1 p 27 total RNA
34. 1 5 2mm 3 gt LRT 2 ME 500l gt MicroSmash MS 100 3 800rpm 300 X1 300 X3 lt b gt TissueLyser GOHz 5 X 3 p 12 I lt 4 gt 17 000Xg gt 15 000rpm 3 1 5ml 385 lt 5 gt SRT 175l 15 62 gt 99 140l 1 10 lt 7 gt lt 8 gt lt 9 gt lt 10 gt 11 gt 18 gt CAS 8 000xg 10 000rpm 1 f WTS WRT 750 f 8 000xXg 10 000rpm 1 WTS Ne DNase 40 l 5 WR
35. A i Si 5 a LRT 2 ME LRT LRT 1ml 10I 2 WHT 2 ME 1 1 gt CAS WRT 750 SRHT SRT gt 899 8 000xg 10 000rpm 1 gt SRT WTS 1 5ml _ 0 0 gt 899 ANG o 0 WENO2a IM2RNIE NGO
36. lt a gt MicroSmash MS 100 lt b gt TissueLyser 3 800rpm 300 30Hz 5 3 800rpm 300 x3 e e e 5mm lt 4 gt lt 5 gt 6 gt lt lt 8 gt 17 000xg 15 000rpm 3 385 1 5ml
37. p 4 5mm RNA LHRT 2 ME LRT 2 ME 500 3 3 p 4 LRT 3 15 30mg
38. DNase DNase 3 p 4 DNase DNase DNase DNa se CAS H DNase DNase DNase 15 58C 5 DNase DNase DNase 8 15 88C 37C 9 WTS 8B 000xg 8 000xg
39. 4 5 15mg WHT lt a gt lt b gt lt 9 gt DNase DNase MIET6SBSh MS 100 TissueLyser DNase 10 gt DNase p 4 3 800rpm 120 30Hz 5 X lt 9 1 gt DNase 3 800rpm 300 Cat No 2 RQ1 RNase Free DNase Promega M6101 i A Deoxyribonuclease RT Grade 313 03161 DNase I RHNase Free Ambion 40U 40 ul OAI RNase Free DNase Set QIAGEN 7g254 3 i lt 4 gt 17 000Xg 15 000rpm 3 1 500Kunitz units
40. 1 5ml POC 80C CRHT 2 20 21 22 CRT 50 l CRT 50 4 1 p 27 15 28C CAS DNase DNase DNase DNase
41. 10 000rpm CAS 3 RNA WRT 750 I 3 SHT SRT gt 9996 SRT CAS WRT WRT gt 999 8 1 p 8 WRT WRT
42. RT 85ml Solubilization Buffer SRT 40ml 8 2 Wash Buffer WRT 75ml Elution Buffer CRT 100ml 9 PAY SF Cartridges CAS 96 10 Waste Tubes WTS 198 1 QuickGene SP kit RNA tissue SP RT 2 2 15 28C 9 fs 3 e 2 2 ME LRT lt gt 999 WRT e e RNase e 1 5ml RNase e 2 500rpm
43. T 750g i 8 000xg 10 000rpm 1 I WRT 750 i 7 8 000xg 10 000rpm 1 a U 1 5ml Ne CRT 100 l 2 8 000xg 10 000rpm 1 total RNA 12 15 30mg 1 gt gt lt 3 gt 1 p 7 p 7 10mg 5 15mg p 16 1 5 mm
44. a 5 FUJIFILM Life Science N RNA QuickGene SP kit RNA tissue SP RT Ver 1 1 5 30mg total RNA Contents 2 e 30 8 e e total RNA 1 86 total RHNA 5 Lysis Buffer L
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