User's Manual - Turner Designs
Contents
1. ASCII Data Capture Integrator Firmware Campbell Scientific CR1000 Datalogger Integration N O1 WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT WEEE DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well being of our environment Accordingly we are concerned with preserving the surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products WEEE Return Process To arrange the return of an end of life product proceed as follows lf you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at support turnerdesigns com Turner Designs will provide a WEEE RMA Number a Shipping Account Number and a Ship to Address Package and ship the product back to Turner Designs The product will be dealt with per Turner Designs end of life recycling program in an environmentally friendly way 1 1 The C6 Multi Sensor Platform was designed to integrate up to six Cyclops 7 submersible sensors for extended or short term deployments The Ce Multi Sensor Platform provi2. Data gger Toe Cie Fp orba sion Plarm g CADE 1 Direct Connect onmectiom Cob Part TEM LISA bo Serial Boke oC ih Dai ATEGO Bara ci Piste TRY Fri s doien T Se ourity Code Entry Reip rni Tra fs Data File Paika sladu Pimpi lo DORA CR ON ado del Punto OC edb PLA oT a an Cec dza rm bal lari PCS oc 998 2205 Rev N GISA 144 Prd ti 252004 1 24 Prd Pause Clesck Updrie Datso Tires ara Ofar Poop han m alakn gev Propam Lirren Piven I LE ataj ng png v CRI Sered Preji Fenema P reezrarm Page 37 998 2205 alti Sermo Pletform 4 3 Successful compilation of the program by the CR1000 will result in a message box like the one below Click on OK The program was successfully sent to the datalogger CPU C3 Cb Datalogging v CR Compiled in FipelineMode 4 4 Click on the Monitor Data tab Hie Datslogger Hetwork Took Help Kuo REM OO AGA O a Ook Propam Kioto ata oilec Deta l Dialer Cala gaar Plane Tr eT x Griniopger Tae Catal og rar 6 23 2044 1 23 44 Pd Farm cba Se on d ac be b 1 4 Prd Sexi Bridge ali Paice Clock Update Catalog oar Ti Tira Sere fari Fah Bus deiner 770 Set otk hours ra Chow Om S LUT N Cela v Eira Fi pe Tie Tire fe Data File Paths A tiun Ci armpba lx POZDOVZFIDOO latin dal Liaren Prej Po A b c POZVAL ECO Pu tdi dl i CE Degn ve CR Ei Ola m bahi PAR Cd
3. Serj Friji Fame Pregn Rev N Page 38 Molti iensor Plethorm 4 5 The GUI will change to reflect the image below e The RecNum field is representative of record number This value should be increasing at a rate of 1 every 10 seconds e The TimeStamp is the time of the measurement as kept by the CR1000 This timestamp is independent of the timestamp delivered by the C6 which is contained in the RawString field e The RawString field contains the serial string as delivered by the C6 SplitData fields are the parsed records from the Ce serial data string e SplitData 1 through SplitData 6 represent channels 1 through 6 e SplitData 7 and SplitData 8 represent depth and temperature respectively Fie ODwtalegger Hetbwork Took Help Ku 33 HD Bos 0 M s i Clock Progam Perii Dit Coed Date Deele Por Deimina Z ypdte tient mne cnie ead Rechum d d TimeStamp A 143155 Raw trng 10 24 61 SpleDaia i 256 Zplilata i44 Spi ala 3 17782 l Zpli zl2 4 ag l Spliliata 5 24 61 l Spli zlat HAM l Spi ata 7 He l Split zla HA l TimeStr Hi 14 3154 l Spell 180 bbjesReu 6200 l 4 6 For reference the serial data string coming from a C6 into a terminal program is displayed below Pi Edt Foret Vas F n Dent tee I bl Hl One 11145405 KEETE TL 43 i Ce 11 14 57 FETE T1 44 0 Ifa 11
4. over discharge over heating short Protection Features Sot circuiting 998 2205 Rev N Page 26 Appendix B Recommended Practices Description The Turner Designs C6 Multi Sensor Platform can be used with Cyclops 7 sensors for many different applications It can also be integrated into a multi parameter system It is capable of logging and storing data and uses algorithms to compute direct concentrations of fluorophores Identification Each Cyclops 7 Sensor is identified by a letter stamped into the connector C Chlorophyll U CDOM FDOM O Crude Oil F Fluorescein B Optical Brighteners P Phycocyanin E Phycoerythrin R Rhodamine A PTSA T Turbidity and G Refined Oil Fuels Recommended Measurement Practices The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels 1 Use a dark plastic or glass container for your standards and or samples Note Some plastics may fluoresce and might interfere with measurements 2 If using a glass beaker place it on a non reflective surface preferably black 3 Ensure that the sensor is more than 3 inches above the bottom of the container 4 Ensure that the sensor is in the center of the container and has more than 2 inches clearance between the sensor and the inside surface of the container 5 If using a glass beaker check if the
5. Cute g mr Harm CFIOOOI Coto er Tape FP Diii Cohen Connection COR Fort COMMIS atalonne Settings Baud Arin Se Pad ri Address 720 Security Cade Esti Aaen Tire Oh Dota fie Pathe Thua ed Pee CA as cae Peal bes CCa pal POIDOV CFT Full c dat EE I O pd A A FRAN A cist 998 2205 Rev N Chicky Datakegger Pax mi lock Upaste spp raida ena OHA ent Cimet Pro ze I A Detelegging si AK Page 34 Molti iensor Plethorm 2 3 Anew window will open titled Device Configuration Utility 2 06 Under Device Type in the left panel highlight the CR1000 Select the correct com port then click Connect Fle Crilor Hap thc R D bend 08 MH Carmara nie CR1000 h onder to configura ite CATON prar 12 Volts DC must Be suppiad ta tha daialogg4r on a Powe In par A nina pin cabh should also b connected betesen one of your computers AS 2S Pods ard the AS 242 por on the daidiogga When these epimers hove bean met sil ci the appropria serial pan in ite We pened and peas the Commect Button 2 4 A warning message will come up asking you to bypass checking for com port conflicts with the local server click Bypass The warning message window will close Within the remaining Device Configuration Utility 2 06 window click on the ComPorts Settings tab File Options Hep Cavia Typa Damen e Control ais Monee Reco Sends iip G
6. Page 30 G Plethorim Most applications will integrate streaming data Below are instructions on how to save streaming data on a PC To save data 1 Onthe HyperTerminal window toolbar choose Transfer and then Capture Text File Edit Yew Call Transfer Help Send File Dias lgj a Serie Send Text File Capture to Printer 2 When you click on Capture Text a window will appear asking you to name and save the txt file to the location of interest aj x Falder C Documents and File ETE TE Browse r 3 Once you are finished you will need to return to the HyperTerminal window toolbar choose Transfer and then Capture Text and select Stop This will stop data capture to the txt file Ele Edit z m er Help HAF sed Fie Send Text File Capture bo Printer 998 2205 Rev N Page 31 Appendix F Integrator Firmware Turner Designs offers Integrator Firmware This firmware is designed for use by customers that are integrating their C6 with an external datalogger CTD or similar system The Integrator Firmware is different from the standard Ce firmware and has some features that users need to consider before installing 1 Internal datalogging is NOT possible with this version of firmware The Integrator Firmware Is intended for data output only 2 The data output rate defined by this Integrator Firmware is 1 second and cannot b
7. Ti Chan Chlorophyll a LAKE Retrieve Unit Time Chan 3 V Phycocyanin Chan 4 Current Time 1032127 Set Unit To PC Dake Time Chan 5 ORSR AME Chan 6 Depth fv Manually Set Unit Date amp Time Temp Set Unit Clack 3 2 2 Current Data and Calibration Screen The Current Data Screen allows users to view real time data acquisition in both tabular and waveform formats as well as conduct calibrations for each channel and view calibration information Current Data Sensor labels are displayed but cannot be changed in the Current Data screen Real time values are displayed next to each channel and in the adjacent Data Table A calibration button is available for all Cyclops 7 sensors and the depth sensor Real time data can be saved by selecting the Start Data Capture button Users will be prompted to enter a file name and file location Data files will be saved in csv format Tabular Screen Real time data including date time units and temperature are continuously scrolling on the tabular screen for enabled sensors 998 2205 Rev N Page 12 PAUIEI zz r ir Plethorm Each sensor identification name will appear in the associated column headings If units are not selected during calibration a relative fluorescence units RFU label will appear ata Table Diep Miratana f Tire A Emini F h Tere Bebis Curar Daka Lig Setup Denied hata eae Aui ode nat aid Eha
8. information Solid Secondary Standard SSS for UV Sensors CDOM FDOM Optical Brighteners Refined Fuels and Crude Oil P N 2100 904 See Accessory Instructions on the USB Flash Drive for more information Note Solid Secondary Standard not available for turbidity Booster Kit P N 2200 900 Booster 1 Note Required for cable lengths greater than 10 meters Extender Cables a 10 meters P N 105 2595 25 meters P N 105 2596 50 meters P N 105 2597 Custom lengths available upon request Booster 2 24 Pigtail Interface Cable with Locking Sleeve P N 2200 170 998 2205 Rev N Page 6 High Capacity Submersible Battery with Charger and Cable Battery Bracket Flow Cap Mechanical Wiper Submersible battery Shade Cap 2 3 Installing Cyclops 7 Sensors Up to six Turner Designs Cyclops 7 sensors can be installed onto the C6 Multi Sensor Platform The center port is intended for the optional mechanical wiper only Follow the instructions below to successfully integrate your Cyclops 7 sensor 1 Place the fastener on one of the Cyclops 7 ports of the C6 Multi Sensor Platform and rotate to tighten Make sure not to over tighten 2 Align the 6 pin male end of the Cyclops 7 sensor with the C6 Multi Sensor Platform port and fastener and press in the sensor 3 Hold the Cyclops 7 in place and rotate the remaining ring on the fastener to tighten This will lock the Cyc
9. 14 771 EYE 11 44 18 ia 11 45 11 Bega Wiz Er 11145413 k ra 11 45 14 BEBE 1i 44 15 AGG 11 14 15 HEZE 11 44 17 UT 11 44 11 EYE 11 44 19 0A Des fe 11145471 SR 11 45 22 Se 11 41 24 SEEE gA T E b bD A a a GEE A 0 0 4 1 E D 1 22 SERESGRTTZEL STRAT IESE SESS M A ll ol gl ol n n 4 j j SERERBHESZERREC RRL EEER EE ee ee m Bi 998 2205 Rev N Page 39
10. R1000 However the C6 requires RS 232 logic levels and therefore it will need to occupy the RS 232 port As a result the PC will communicate with the CR1000 using COM1 on the lowest terminal block In order for the PC and the CR1000 to communicate your PC will need a serial port The only pins used on the DB9 serial connector are 2 3 and 5 DB9 Connector from PC CR1000 COM1 TX C1 COM1 RX C2 998 2205 Rev N Page 33 Molti Sensor Plechorm 2 Configuring the RS 232 port on the CR1000 2 1 After the CR1000 is powered and wired to your PC as described in section 1 connect to the CR1000 by clicking on Connect in the upper left corner of the PC200W Datalogger Support Software File Dobre Meigi Tom Help X re ARA HO Bom ClocbsProgram drnitor a a Collect Cate Datalogge Infeemation Duta oy mr Harm CFO Pept e pe Typs A Direct Connect Connection CORA Pert CORALS atalonne Settings Brud Arin Sen Pain dirim 770 Teip Code Eria Bago Tire Oh ata de Pathe O Ded Pe eA ae a Publis Clare pba ech IDA ENE Pub lic chat EE U pd a A F N A d Chocks Datakegger Pe C Pause Chock Upd ie ea enu SHH eat Curent Pager I A Detelegging r AK 2 2 Double click on the Device Configuration Utility icon in the toolbar of PC200W ja Candogge Network Tools Help We comect x fe kal agai Ana S O ClecksProgrm Monitor Data Collect Cute Datalogge lnfeemation
11. TURNER oS DESIGNS Reliable Instruments for an Unreliable World Multi Sensor Platform User s Manual January 21 2015 P N 998 2205 Revision N TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 Table of Contents 1 Introduction 1 1 Introduction 1 2 Important Note Regarding the Use of the Fluorescein Sensor 2 Inspection and Setup 2 1 Instrument Checklist 2 2 Optional Accessories 2 3 Installing the Cyclops 7 Sensors 3 C Soft Windows User Interface 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 3 1 2 Software Installation 3 1 3 Integration Interface Adapter Cable Installation 3 1 4 PC Software Interface 3 2 Software Operation 3 2 1 Settings Screen 3 2 2 Current Data and Calibration Screen 3 3 Calibration Procedures 3 3 1 Direct Concentration Calibration 3 3 2 Raw Fluorescence Calibration Blank Subtracted 3 3 3 Raw Fluorescence Calibration 3 3 4 Depth Calibration 3 4 Log Setup Screen 3 5 Enabling Data Output Ready State 3 6 Downloading and File Management 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing 4 1 2 Care for the Bulkhead Connector 4 1 3 Care for the Sensor Ports 4 2 Warranty Terms 4 3 Warranty Service 4 4 Out of Warranty Service Appendices Specifications Recommended Practices Wiring Guide QmMOMdW gt Linear Range Quenching and Temperature Considerations How to Set up HyperTerminal for Digital
12. Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for incidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state Damage incurred in shipping is not covered Rev N Page 23 4 3 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com Carry out any adjustments or tests as suggested by the Technical Support Department If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write the RMA number on the outside of the shipping carton and ship the instrument orepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in th
13. ables connected and the null modem device added 998 2205 Page 36 G Plethorim 4 Loading the CRBasic program onto the CR1000 using PC200W software 4 1 After the CR1000 is powered and wired to your PC as described in section 1 connect to the CR1000 by clicking on Connect in the upper left corner of the PC200W Datalogger Support Software Duty Mebenk Taos Help sri SS HA Bam Cioch Progam fiontar a a Colect Dake Datal gae Infeematian Data g mr Harm CRT Denial e ET Typs FA Dene Conneet Confection COR Fort CORAIS atalonner Settings Baie Arit So Pad ri Adre 720 Security Code 0 Esti Fagg ore Tire Oh Data Pile Path 5 Taa CO ee b CO POZVAL TEE Sab dat Public Clara pois bi POZDOVOLFIOOO Pub bie da TE sr piina AR C c Chic ks atala ger a Paws Clock Update Datalegger Tene Zona HH ateogner Pegram Curent Pray ca CE Detaloggng 3 CR 4 2 After connecting the GUI will display a Connection Time in the lower right corner Click on the Send Program button The delivery of the CR1 file will cause all data on the CR1000 to be deleted The GUI will prompt you if this is OK Click Yes Navigate to the directory where you have the C3 Ce Datalogging v2 CRI file highlight it and then click Open Fie Dutalogger Network Tzk Hap Kum 3 3 HD BAB O Clock Progam Mentor Dota Collect Dots D Will belo ret Beet Detak par Mena CAO
14. d reconnected please allow 100 seconds for the data stream to begin Note Check the optical face when power is supplied to make sure the sensors light The sensor will turn on then off and then turn back on in 100 seconds If this sequence does not happen cycle power The C6 Multi Sensor Platform s output is in digital ASCII format 3 6 Downloading and File Management Users can download new and existing files saved in the C6 Multi Sensor Platform Select Download Data Note This step may take several minutes depending on file size 998 2205 Rev N Page 21 amp lti ferngor Plettorm When download has completed choose the files to be saved by clicking the Save box Users will be prompted to choose a folder location where data will be saved Files will be saved in a CSV format Pile aiig O Port j Halip Settings Current ata Logtmtup Downin Dota Select files to save Tota Fe ip Led Data Log ove nae rary icant a a ln m 41001 A FH A in Dornia a u O Canalo Select Clear Datalog to erase existing data from memory Users are able to upload data and view in the Current Data window if desired Note If you have problems downloading data in C Soft you may have limited space in the cache memory on your computer We recommend you do a Disk Cleanup to see if that solves the problem and allows you to download files Disk Cleanup can be found in the followi
15. des automatic gain control calibration data logging and digital data output for each Cyclops 7 sensor The C6 Multi Sensor Platform comes with factory installed temperature and pressure sensors and is rated to a depth of 600 meters The C Soft Windows based user interface allows for intuitive calibration data logging set up and file downloading Existing Cyclops 7 users can easily integrate their sensors into the C6 Multi Sensor Platform 1 Introduction 1 2 Important Note Regarding Use of the Fluorescein Sensor For C7 s manufactured prior to 2 15 2011 the Fluorescein sensor should not be enabled for simultaneous data collection with Chlorophyll and Rhodamine sensors or it will produce unreliable results For C7 s manufactured after 2 15 2011 this interference was designed out If you would like to send your C7 back to Turner Designs to resolve this please contact sales turnerdesigns com 998 2205 Rev N Page 4 G Plathorm 2 Inspection and Setup 2 1 Instrument Checklist The C6 Multi Sensor Platform package P N 2200 000 includes Ce Multi Sensor Platform Factory installed pressure sensor Factory installed temperature sensor Computer Interface cable with 12V power supply Integration Interface Adapter Cable see Appendix F and G for more information about integration Sensor fasteners 8 pin female plug USB Flash Drive including C Soft software user s manual cable guide and quick sta
16. dn Taran R Daop Confort Settings TORI CELO PPP retwor Servings advances CRNE R ENPE CRNETO SARUN Seamty Code Hi CRIK Pak um arity Security Ceda 1 zmaxrty odm 7 ORINA Pate Gopi Ree ORITETO Corim Pafin Preryptirn Kay Station Kadet Specifics a name assigned to his siat on This valia is raad orty Piha daia is carant minning a program wih CazdDut insiructions 998 2205 Rev N Page 35 G 2 5 Next to Select the ComPort click the dropdown menu and select RS 232 Next select a Baud Rate of 9600 Fixed Then click Apply At this point you are done with the Device Configuration Utility Click on Disconnect Station Name Species a name aesigned to the slation Thes value te read onhy Tha dalaopger IE curently running a prodam wih axd ur ii metruchons 3 Connecting the C6 to the CR1000 3 1 The C6 uses RS 232 logic levels therefore communication between the CR1000 and the C6 must be on the RS 232 port of the CR1000 The configuration of this port is described in section 2 Connect the 8 pin female underwater connector end of P N 2200 150 to the Ce Connect 9 pin female DB9 end of P N 2200 150 to the male 9 pin DBY on P N 2300 115 3 2 Between the C6 Communication and Integration cable P N 2300 115 and the CR1000 RS 232 port you must add a null modem device The image below shows the C6 instrument with the proper c
17. e changed to reflect longer or shorter data output rates 3 Once power is applied there is a 15 second delay before data output starts at a 1 second interval 4 Ifthe wiper is enabled it will rotate the set number of rotations upon powering the instrument and every 5 minutes as long as power is continuously supplied 5 Sleep mode is disabled therefore as long as power is continuously supplied the instrument will continue to stream data at a 1 second interval Download Firmware on the Turner Designs website http www turnerdesigns com customer care software a firmware 998 2205 Rev N Page 32 G Plethorim Appendix G Campbell Scientific CR1000 Datalogger Integration This document assumes you have experience connecting to your CR1000 The procedure of interfacing the C6 Multi Sensor Platform is described in four sections Wiring the CR1000 to aPC Configuring the RS 232 port on the CR1000 Connecting the C6 to the CR1000 Loading the CRBasic program C3 _C6 Datalogging v2 CR1 onto the CR1000 This file can be found on our website at the following address htip www turnerdesigns com t2 sw C3 Ce Datalogging CR1 zip Note Sections 2 and 4 reguire Campbell Scientific PC200W software Campbell Scientific PC200W software can be found at the following web address htip www campbellsci com downloads sb PC200W amp c 9999 1 Wiring the CR1000 to a PC Typical communication between a PC and a CR1000 uses the RS 232 port on the C
18. e contiguous continental United States For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 4 4 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2205 Rev N Page 24 998 2205 Rev N Page 25 Appendix A Specifications Ce Multi Sensor Platform Weight in Air 2 74 kg 6 0 Ibs without sensors submersible battery or wiper Weight In Water 1 6 kg 3 4 Ibs Length 29 8 cm 10 2 in 10 2 in Length with Sensors Attached 33 8 cm 13 3 in Signal Output Digital ASCII 79 In Diameter 7 cm 2 75 in Temperature 2 to 50 Depth 0 600 meters Capacity 2 200 mAH Voltage Output 14 8V nominal Protects against overcharge
19. entrations after calibrating to Direct Concentration Mode iS Standard Value units x Sample RFU Concentration units Standard RFU Raw Fluorescence Mode Blank Subtracted Values in the Raw Fluorescence Blank Subtracted Mode are noted as RFUB It is important to note that these values are blank subtracted but are not scaled to a standard they are relative values Raw Fluorescence Mode Values in the Raw Fluorescence Mode are referred to as Relative Fluorescence Units RFU It is important to note that these values are not blank subtracted or scaled to a standard they are relative values 3 3 Calibration Procedures 3 3 1 998 2205 Direct Concentration Calibration Following steps 1 5 will ensure values are reported as Blank Subtracted Concentrations in units of choice See Appendix B for recommended practices Step 1 Blanking Place the C6 Multi Sensor Platform and Cyclops 7 sensors in a blank solution Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial seawater or filtered seawater Note Ensure the Cyclops 7 sensors are free of air bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Standard Value and Units Rev N Page 15 Place the C6 Multi Sensor Platform and Cyclops 7 sensors ina sta
20. g message asking if you want to unblock messages select Unblock 3 1 3 Integration Interface Adapter Cable Installation 1 998 2205 Insert the USB Flash Drive and install the Adapter Cable driver Integration Interface_Adapter_Drivers exe onto your PC Make sure nothing is plugged into the Adapter Cable s connectors Plug the USB connector into a USB port on your computer and wait for hardware to automatically install Once USB is installed and ready to use right click the My Computer Icon Note For Windows Vista or 7 users open the start menu and right click on Computer Left click properties to open the properties window If running Windows XP or older versions of Windows click on the Hardware tab and then click Device Manager to open the list of devices installed on your computer Note For Windows Vista or 7 Rev N Page 9 998 2205 10 users the Device Manager link is located in the upper left part of the properties window you will not see a Hardware tab Scroll down and open the Ports heading from the list 8 The newly installed Adapter will be titled USB Serial Port followed by a COM port designation in parentheses for example COM 5 If the USB Serial Ports COM designation is labeled as any number from 1 6 your installation is complete Close all windows and make sure to set C Soft to that specified COM designation when trying to connect via USB Adapte
21. he Wiper Box to enable wiper if the wiper is not installed C Soft Manager x The sampling interval is too short for the number of wiper revolutions You must choose a longer sampling time lass revolutions or turn the wiper oft Sampling Maximum Interval of Revs 1 10 0 1 Z 31 40 3 4 a 10 Cancel After log set up is complete select Enable Datalog See section 3 5 Enabling Data Output Ready State A prompt will appear asking if user is ready to enable logging select Yes Note If data logging is set to start for a time in the past there will be a minimum delay of 100 seconds before startup after power has been supplied Otherwise wait until the data logging start time has been reached for data logging to begin WARNING As soon as data logging is enabled users will be unable to conduct calibrations or view current data This should be the last step before the C6 Multi Sensor Platform is deployed 998 2205 Rev N Page 20 3 5 Enabling Data Output Ready State The Data Output Ready State must be enabled for the Ce Multi Sensor Platform in order to log data capture real time data output and or integrate the C6 with other instrumentation See Appendix F and G for more information about integration There are five necessary steps to ensure the instrument is properly set up for data output 1 Set the instrument s clock to PC or local time in the Settings Tab 2 Select the sensor
22. ibration is complete select Save Calibration Data are now displayed as concentration estimates 3 3 2 Raw Fluorescence Calibration Blank Subtracted Following steps 1 5 will ensure values are reported as Blank subtracted Relative Fluorescence Units RFUB See Appendix B for recommended practices Step 1 Blanking Place the C6 Multi Sensor Platform and Cyclops 7 sensors in a blank solution Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial seawater or filtered seawater Note Ensure the Cyclops 7 sensors are free of bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Selecting Units Select RFUB from the units dropdown menu Note Do not enter a value in the standard value box Step 3 Skip and go to Step 4 Step 4 Optional Temperature Compensation Follow same steps under the Direct Calibration procedure in section 3 3 1 Step 5 Save Calibration After calibration is complete select Save Calibration Data are now displayed as blank corrected relative fluorescence units 998 2205 Rev N Page 17 3 3 3 Raw Fluorescence Calibration In the Current Data screen select the calibrate button next to a specific channel At the bottom of the Calibration Screen select Click to
23. j TAAN tu pH n wea ted Ou 2147 Sarees Menim ae NL ZDNNLI TEN slisl ame ho EE O00 TEL Chet Rhode my a alana 10200 M Mae lom HA mmn 15 aa 4 0 00 TE CE a aa 0M id DAJ LANA EALE 11 28 74 20 2147 Own 3 porn ThE bbe gs Baliabi i bu A Tim PR i 2i t Fly Oates MESNA H PR KI 2i 47 KLIBNA 1 Soe aka 7a 4 Ha 2147 Gach a P da id COTENT 73 28 100 EER e T 10 E mi mal a 21 Fas Rat eM 10 3s 0 Paci rar Had abr ins S S om EEN COEIRE wiz 72 2i t Depth testers a Cie Waa 113 aa ma 9 5 LA COCE yA rari Ou 21 TEM CA ELE CAT ise PLA iF oli il 3616 RA E EL 21 5 alia AO A 0 00 TE CAE My Mis i NBEZNITENE Fake HH zlo CIE MEAN ie feed Ha 2i p Bo Le L Ea FH pes LI 21 te tarot bata TCT set aaa mite u EEJ CERE NH ise Hu s Fa Po 10 35124 Fe Les 7a ss 21 Faz ee AB 000 TE St Deby Caphue Mel pea hes pO 3 2h S a fete Ha zl CEE a5 ad LELI 21 7a ini e AO 0 140 TEE aa wa E 1a T 1 e Wast Pi ceestonws A ber sd ne U r isamaa EF Rr eS A ae A in Waveform Screen Users can graphically view real time data for each sensor by clicking the Display Waveform box Sensor identification name and units will be displayed on the Y axis Time and date will be displayed on the X axis Clicking the Autoscale box will automatically scale graphs Users may also rescale graph
24. kground On an MS Windows computer open the HyperTerminal program Start Programs Accessories Communications HyperTerminal 1 The Connection Description screen will appear Name the connection description This file will save the communication parameters for the C6 Multi Sensor Platform and can be used in the future to establish communication with the instrument quickly 2 The Connect To screen will appear Choose the appropriate communications port in the Connect Using window Click OK 3 The Port Settings screen will appear Choose the following port settings Bits per second 9600 COMI Properties RE Data bits 8 Port Seting Parity None Stop bits 1 ity nt AH Flow control Hardware D te F Click Apply and OK Pair Hors Z Soobisfi 753 Frey contok H udara z Acton Dalsia sed dv 4 Next the HyperTerminal window will appear You are now ready to connect the C6 Multi Sensor Platform to a power source Data will be displayed at set intervals in the HyperTerminal window after 100 seconds or at the specified log start time ipi z Fa Efi Yew Cal Tear Help Dij S15 OE oF ata lime HhodasinePhacecvani Uepth Temp 4 6 16 08 15 07 24 10 0 129 7 3 71 Aliga Ta Bl 31 43 La 1 O f 23 f2 1606 15 07 11 41 130 143 0 0 23 72 bl 12 07 42 wl ta 1 es 0 0 6 16 08 15 07 24 51 09 13 5 6 6 23 72 blb Tachi a1 Ai LAY B B 29 fe 616 08 15 07 11 23 131 18 0 00 73 72 998 2205 Rev N
25. lops 7 into position 4 Follow the same installation instructions for the remaining Cyclops 7 sensors 5 Install a 6 pin male plug onto any unused port on the C6 Multi Sensor Platform NOTE To protect your bulkhead connectors from damage a light coat of Silicone spray should be used on all of the rubber bulkheads and pins to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray You should avoid using silicone grease DO NOT USE WD 40 it will destroy the connectors 998 2205 Rev N Page 7 G Ple armi Cyclops 7 Fastener 998 2205 Rev N Page 8 3 C Soft Windows User Interface The C Soft Windows based user interface allows for intuitive calibration data logging set up and download functions 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 4 133MHz microprocessor 600 X 800 VGA resolution Microsoft Windows 98 32 MB RAM 3 1 2 Software Installation 1 2 Exit all Windows programs Insert the USB Flash Drive also located on the Turner Designs Software Firmware webpage under Customer Care Open the C Soft software folder and double click on the setup icon The software will automatically be installed in the PC s C Program Files C6 folder unless user selects alternate location After restarting the C Soft icon is located on the desktop Gott 5aft Note You may receive a warnin
26. ndard solution for the specific sensor being calibrated or use a solid secondary standard See Accessory Instructions on the USB Flash Drive for additional information Note Turbidity sensors do not have solid secondary standards Enter the known concentration value of the standard solution or solid standard in the Standard Value box If the concentration is not Known enter an arbitrary value Enter manually or select desired units from the dropdown menu Note Do not choose or enter RFUB if Direct Concentration Mode Is used Step 3 Set Standard When readings in Current RFU box have stabilized select the Set Standard button Step 4 Optional Temperature Compensation Temperature compensation is optional it enables the C6 to compensate for changes in fluorescence due to varying temperatures as detected by the C6 s temperature sensor Temperature compensation is available for in vivo Chlorophyll and Rhodamine sensors only Temperature is displayed in degree Celsius Click on the Temperature Compensation box Select either in vivo Chlorophyll or Rhodamine from the dropdown menu Data will now be corrected for temperature fluctuations The temperature compensation coefficients for each fluorophore are listed below Fluorophore Coefficient 0 026 C Exponential in vivo Chlorophyll 1 4 C Linear 998 2205 Rev N Page 16 Step 5 Save Calibration After cal
27. ng location on most Windows versions Start Menu gt Programs gt Accessories gt System Tools gt Disk Cleanup After the initial scan of the computer completes customers should select all the boxes and select OK to perform the cleanup 998 2205 Rev N Page 22 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing The C6 Multi Sensor Platform should be rinsed or soaked in fresh water following each deployment Care for the bulkhead connector Install the 8 pin female end plug to the bulkhead between uses A light coat of Silicone spray should be used on the rubber of the male pins of the bulkhead to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray Note You should avoid using silicone grease Do NOT use WD 40 it will destroy the connectors Care for the sensor ports Install 6 pin male plugs onto any vacant port A light coat of Silicone spray should be used on the rubber of the male pins of the bulkhead to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray Note You should avoid using silicone grease Do not use WD 40 It will destroy the connectors 4 2 Warranty Terms Turner Designs warrants the C6 and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions 998 2205
28. offset only Step 2 Step 3 Select Save Calibration button Future depth values will be scaled to the offset selected Atmospheric and Gravity Offset Method 2 Step 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Select Atmospheric and gravity offset Enter the latitude Step 3 Select Save Calibration button Future depth values will be scaled to the offset selected 3 4 Log Setup Screen The Log Setup screen allows the user to configure a logging session set the mechanical wiper to its home position and enable logging The C6 Multi Sensor Platform can log over 60 000 lines of data Enter a log start date MM DD YYYY and time HH MM SS Comet 3 Saiting Ceca ha Le Stbep feaabeodiiete Enter the sampling interval HH MM SS The minimum sampling interval is 1 second 998 2205 Rev N Page 19 G If the mechanical wiper is installed click the Wiper box to enable Select the number of revolutions from the dropdown box 1 10 revolutions All sensors will be wiped at specified revolutions before each measurement Note If users select revolutions that exceed sampling interval a warning message will be displayed Note Do not click t
29. on process to complete Rev N Page 10 T amp lti ferngor Plettorm The connection icon located on the upper right of the screen will turn green if connected C Soft Manager File Settings COM Pork Window Help Connected 2 Note If the connection icon does not turn green disconnect and reconnect power 3 2 Software Operation 3 2 1 998 2205 Settings Screen The Settings Screen allows users to set the time and date identify each Cyclops 7 update serial numbers and select sensors to be enabled Users can manually set the date and time or choose to synchronize the C6 Multi Sensor Platform to the PC date and time Each channel 1 6 in the Settings Screen has a port associated with it on the C6 Multi Sensor Platform that is numerically labeled 1 6 respectively Click on the box adjacent to the channel to enable channel operation lf there is no sensor installed users will be unable to select that channel Channels can be renamed using the dropdown selections or by manually entering a name Channels can also be named using Cyclops 7 serial numbers Settings will automatically be saved after exiting the Settings screen Rev N Page 11 C Soft Manager S File Settings COM Port window Help Connected 2 settings Current Data Log Setup Download Data C Soft Manager a a Channel Settings Enable Name Serial Chani M Rhodamine Unit Current Time rhan 10 32 20 i it
30. ons where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is known as signal quenching Linearity can be checked by diluting a sample 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Fluorometer Response Curve Sample Quenching Region Graph showing Linear and Quenching Regions of the sample s response f Sample __ Linear Region Sample Conce Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature For further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll please refer to the application section of Turner Designs website 998 2205 Rev N Page 29 5 sensor Plat mr mi Appendix E How to set up HyperTerminal for Digital ASCII Data Capture Ensure C Soft program is not running in the bac
31. optical surface of the sensor is free of air bubbles Remove air bubbles if any are observed If using a dark plastic container run the wiper or your finger over the optical sensors to ensure the sensor heads are free of air bubbles Calibrated Se s gt Inches all round Glass Beaker 998 2205 Rev N Page 27 Appendix C Wiring Guide The C6 Multi Sensor Platform outputs digital data in a format that can be read by C Soft software or a terminal emulator program It can be integrated into larger multi parameter systems such as CTDs or buoys refer to Appendix F and G for additional information An 8 pin impulse cable provides two end connections 1 a 9 pin RS282 serial port for connection to a PC or laptop computer and 2 a 12V port for supplying power to the unit Ce Multi Sensor Platform bulkhead and serial port connectors Sub D Connector GOO OO Wire Guide ee aa eee eaaa a pe m ew a TI 998 2205 Rev N Page 28 G Plachorm Appendix D Linear Range Quenching and Temperature Considerations The linear range Is the concentration range in which the Cyclops 7 output is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrati
32. r Cable connection If the USB Serial Ports COM designation is greater than 6 right click on the USB Serial Port and click properties which will open up the properties menu From that menu click on the Port Setting tab and then click Advanced which will open the advanced settings menu for that USB Serial Port Choose any COM port 1 6 from the COM port pull down menu and click OK Note Ideally you want to start with any port that is not in use if all are in use then choose any port 1 6 Click OK to the warning box if It appears and Click OK to close any other open menus Your installation is complete Make sure to set C Soft to that specified COM designation when trying to connect via USB Adapter Cable connection PC Software Interface Note It is important that the steps be followed INORDER Any variance from the procedure may result in the connection NOT being established 1 Plug either the USB or serial connector of the adapter cable into your computer Note Either connection can be used for communicating and configuring the instrument Plug the interface cable onto the 8 pin bulkhead connector of the instrument Plug the interface cable s serial plug into the adapter cable s male serial plug Begin C Soft by double clicking on the C Soft icon and choose the appropriate COM port from the pull down COM menu Plug the 12V power supply into a power source 6 Wait for initializati
33. rt guide Temperature sensor Sensor fastener i Eyelift holes Bes 1 8 pin female end plug Pressure sensor Integration Interface Adapter Cable Computer Interface Cable 998 2205 Rev N Page 5 G Rechargeable Submersible Battery Pack including charger P N 2200 601 See Accessory Instructions on the USB Flash Drive for more information Battery Bracket P N 2200 603 See Accessory Instructions on the USB Flash Drive for more information 2 2 Optional Accessories Note Required for use with Battery Pack and Analog Output Adaptor High Capacity Submersible Battery with Charger and Cable P N 3200 600 See Accessory Instructions on the USB Flash Drive for more information C6 Mechanical Wiper P N 2200 450 and Replacement Brushes P N 2200 460 See Accessory Instructions on the USB Flash Drive for more information 6 pin male plugs P N 105 2210 6 pin male plug Flow Cap P N 2200 700 See Accessory Instructions on the USB Flash Drive for more information Shade Cap P N 2200 510 See Accessory Instructions on the USB Flash Drive for more information Note We recommend use of the shade cap as it provides a fixed distance for calibration and sample measurement and minimizes effects from ambient light Solid Secondary Standard SSS for in vivo Chlorophyll Phycocyanin Phycoerythrin Rhodamine and Fluorescein P N 2100 900 See Accessory Instructions on the USB Flash Drive for more
34. s by unchecking the Autoscale box and manually entering desired values for either Y or X axis ranges Data can be saved during waveform mode by clicking Start Data Capture Data files will be saved as csv format 998 2205 Rev N Page 13 re inp COM Pa Bg E Multk ensor Platform rihap Deron ua lom rip Nazval Pid Dreri Ciadu E hiami a 115 E alis m s eee 111 alia ud mi Lurre wE 1 ul mie IOM j A Cale he Firs I at aku u Wo dne an reim caliente Tare Li Tern Sails glo Coe Hlad Pele Sapir Calibration Screen Users can calibrate Cyclops 7 sensors in either the direct concentration mode raw fluorescence mode or blank subtracted raw fluorescence mode 998 2205 Char weed ad bration QnitLeted Toi 1 Rhodamine Step Pres Get Blank bo sat the bland Hug to the current PAJ Enter the value of pour shana aod select the und of mee Step 3 Press Get Standard to sat te Standard valia bo Hg cuirart AFL ploret Oi the bangers Compensation Dare to aiias She 2 After cabrio i Conde prs NA zr Dit bo un ur abc Flee Pree Pad Rev N Curat PRI 370 Blank FFL Standard RFI Tang Copacati fa Lano Page 14 Direct Concentration Mode Values reported In the Direct Concentration Mode are scaled to a predetermined standard value and blank subtracted The eguation used to calculate conc
35. s you expect to use during sampling 3 Calibrate selected sensors for either Direct Concentration Mode or Raw Fluorescence Units Blanked RFUB Mode see section 3 2 2 4 Set the start time sampling and wiping intervals in the Log Setup Tab Note Wiping interval is optional and depends on the sampling interval see section 3 4 5 Click the Enable Datalog button followed by the Yes button to enable the Data Output Ready State Note Use the Battery Life Calculator available on the Turner Designs website C6 product page to calculate a conservative estimation of how many days you can expect to log data Factors such as battery charge and water temperature will affect logging days A few seconds after the Yes button has been clicked to enable the Data Output Ready State the C Soft program will automatically shut down and users must disconnect power and serial connections The instrument is now in the Data Output Ready State and will remain in that state so long as C Soft is not used to communicate with the instrument Note As soon as C Soft is used to communicate with the instrument users will need to re set the parameter settings in the Log Setup section 3 4 and again enable datalog to put the instrument into the Data Output Ready State When power is supplied to the instrument while it is in the Data Output Ready State it will begin streaming data after a 100 second delay If power is cycled i e disconnected an
36. use uncalibrated Raw Fluorescence Mode Data for that channel will be uncalibrated and reported as Relative Fluorescence Units RFU 3 3 4 Depth Calibration Note Make sure your instrument is calibrated for depth and there is a value set even if you do not have a depth sensor Depth is displayed in meters only There are two calibration options for depth calibration 1 The first uses only the atmospheric pressure as an offset This is suitable for any shallow application including most fresh water applications 2 The second compensates for gravity variations with latitude as designated from the UNESCO Technical Papers in Marine Science 44 http unesdoc unesco org images 0005 000598 059832EB pdf This method assumes 0 C and 35PSU salinity Chari Calibration init Latest Dogi Steg Pres Set Zens to saat te Tic ear eC Steg Z Select a Gesu Ooo meii Steg 3 en Wi ae dro Gis Sara Calibration of frac Cancel al ae Gree bo abat thes Cabra tio Atmospheric Offset Method 1 step 1 Curtedt Preccura NOO Pod Term Reacting ae TET Abncepharic ofan ante r zivisnberk sed gravity offset Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero 998 2205 Rev N Page 18 G Select Atmospheric
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