User's GUide
Contents
1. 0 955 0 964 0 972 0 964 0 958 0 964 0 967 0 964 n number of weighings W weighing results SD 0 011 mg W 0 968 0 960 0 984 0 942 0 969 0 966 0 955 0 972 0 958 0 967 10 W 0 964 mg Random error SDv SD SD xZ SD 0 011 x 1 0032 0 011 pL DIDELMOaN m Nf Apprenvix 2 Z FACTOR The reference calculation equation is P density of air at t C P density of the test liquid at t C P density of the balance weights Use 8 g cc for P Z 1 P P 1 P P Where FS Weights conforming to International Recommendation N 33 of OIML have been adjusted to give results when weighing in air as if the density of the weights were 8 0 g mL Values of the conversion factor Temperature Air pressure hPa Z uL mg as a function of C 800 853 907 960 1013 1067 temperature and pressure for 15 1 0018 10018 1 0019 1 0019 1 0020 1 0020 distilled water 15 5 1 0018 10019 1 0019 1 0020 1 0020 1 0021 16 1 0019 1 0020 1 0020 10021 10021 1 0022 16 5 1 0020 1 0020 10021 1 0022 10022 1 0023 17 1 0021 1 0021 1 0022 10022 10023 1 0023 17 5 1 0022 10022 1 0023 1 0023 10024 1 0024 18 1 0022 1 0023 1 0024 10024 1 0025 1 0025 18 5 1 0023 1 0024 1 0025 10025 10026 1 0026 19 1 0024 1 0025 1 0025 10026 1 0027 1 0027 19 5 1 0025 1 0026 1 0026 10027 10028 1 0028 20 1 0026 1 0027 1 0027 10028 10029 1 0029 20 5 1 0027 1 0028 1 0028 10029 1 0030 1 0030 21 1 0028 1 0029 1 0030 10030 1 002. USER S GUIDE ____ 4G ILSON U pbloetmar rn Nf Contents Page 1 INTRODUCTION 3 2 PARTS CHECK LIST AND ACCESSORIES 4 3 GETTING STARTED 4 4 DESCRIPTION 5 5 PIPETTING JUST LIKE WITH PIPETMAN 6 6 PIPETTING MODES 7 7 PIPETTING SPEED CONTROL 11 8 PIPETMAN DIAMOND TIPS 11 9 GUIDELINES FOR GOOD PIPETTING 12 10 PERSONALIZE YOUR PIPETMAN M 13 11 GOOD LABORATORY PRACTICE FEATURES 14 12 PIPETMAN M POWER MANAGEMENT 15 13 CONFIGURATION 16 14 MAINTENANCE OF YOUR PIPETMAN 17 15 CLEANING AND DECONTAMINATION 22 16 LEAKTEST 24 Warranty 17 TROUBLESHOOTING 24 Gilson warrants this product against defects in material 18 SPECIFICATIONS 25 ei Hil ie foraperiod of 12 months 19 SAFETY PRECAUTIONS AND LIMITATIONS OF USE 16 Gilson is not responsible for incidental damage resulting 20 REPLACEMENT PARTS 26 from physical shock continuous exposure to corrosive APPENDICES 77 reagents or technical handling not in accordance with E TT the guidelines described in the User s Guide EC DECLARATION OF CONFORMITY 31 J USER S GUIDE INTRODUCTION Congratulations on the purchase of PIPETMAN M the natural evolution of PIPETMAN the world standard for pipetting PIPETMAN M is a new motorized member of the PIPETMAN P family from Gilson It is a fusion of PIPETMAN s renowned accuracy precision and robustness as well as user friendly functions to simplify your professional life PIPETMAN M is so easy to use that you will have the
3. Push button and volume adjustment knob 2 Mode button direct access to all menu functions Tip ejector button Display Identity tag clip Identity tag window Connecting nut attaches handle to lower part Tip ejector removable to access piston assembly and tip holder O OHAOOFO Optimized tip holder to reduce tip fitting and ejection forces removable for cleaning and servicing Elastomer plug to cover the battery charging port Reset To reinitialize your pipette simultaneously press on the push 1 and mode button for at least 5 seconds Switch Off To switch off your PIPETMAN M press the push button Q for at least 5 sec Display The PIPETMAN M display is an OLED screen Usually it shows the current mode and operation step pipetting volume battery indica tor and piston status aspirated and dispensed volume in real time IDET Warning messages appear in the place of volume 7 j 200 0 1 Pipetting Mode 2 Indicatorfor aspiration and dispense 3 Battery status Purge 5 Volume Ol peL MaA ay oan BIRSUTITT wa 5 PIPETTING JUST LIKE WITH PIPETMAN Using PIPETMAN Misas easy as using a mechanical PIPETMAN Your PIPETMAN Mode M is set by default on PIPET Mode that means forward pipetting You can PIPET select a volume and start to pipette Switch on Press push button Adjust the volume setting Hold your PIPETMAN M in pipette position e Turn the p
4. you can choose between different pipette modes Select MIX Mode by turning the push button to confirm your selection click on the push button e Setthe first volume named VOLUME 1 click the push button set the volume by turning the push button and click to confirm your selection This is the first volume you will aspirate and dispense Set the second volume named VOLUME 2 click the push button you can now set the second volume by turning the push button Click again to confirm your settings WS You can set the VOLUME 1 or VOLUME 2 0 uL the step will not appear Ifyou set the VOLUME 1 and 2 0 uL the cycle will start directly with the MIX step e Set the MIX volume click the push button set the volume by turning the push button Confirm your settings by a simple click It is the volume which will be aspirated and dispensed repeatedly MIX as long as you press the push button e Set the additional pipetting volume named VOLUME 3 You can choose a volume to be aspirated 8 I USER S GUIDE and dispensed after the mixing step so you can continue pipetting Click the push button set the volume by turning the push button Confirm your settings by a simple click WS you set the VOLUME 3 0 uL the cycle will stop after the MIX step You are ready to mix 1 Fit a tip suitable for the model of PIPETMAN M that you are Caution You must using preferably use PIPETMAN DIAMOND Tips for assurance always fit a tip before
5. you to remove the tip from the liquid without aspirating anything e Eject tip by pressing the ejection button O START button the volume and the last settings LI Optional STOP Your PIPETMAN M is ready again for next pipetting cycle while maintaining You have been pipetting like with your mechanical PIPETMAN and only needed 2 pushes on the push button J USER S GUIDE 6 PIPETTING MODES PIPETMAN M offers more pipette techniques for a large number of applications You will find all pipetting modes in the menu for system settings refer to the Chapters 7 and 13 Access the menu by pushing the Mode button To choose one of the pipetting modes turn the push button and confirm your selection by a simple click Mode button REPETITIVE Mode The REPETITIVE Mode allows you to distribute a Mode same volume repeatedly in predefined number N REPETITIVE of equal aliquots You supply the aliquot volume AV and the pipette automatically calculates the number of aliquots possible from the nominal volume NV of the pipette as follows N NV AV e g 100 uL x 10 for P1000M You also can decrease the number of repetitions e g 100 uL x 10 n gt 21 for P1000M NG Application notes The REPETITIVE Mode is ideal for dispensing aliquots e g dispensing a PCR master mix into PCR tubes or 96 plates pipetting elution buffer for DNA extraction preparing a target for spectrometric analyses ff distributing loading bu
6. 31 1 0031 21 5 1 0030 1 0030 1 0031 1 0031 1 0032 1 0032 22 1 0031 1 0031 1 0032 10032 1 0033 1 0033 22 5 1 0032 1 0032 1 0033 1 0033 1 0034 1 0035 23 1 0033 1 0033 1 0034 10035 10035 1 0036 23 5 1 0034 1 0035 1 0035 1 0036 1 0036 1 0037 24 1 0035 1 0036 1 0036 10037 10038 1 0038 24 5 1 0037 1 0037 1 0038 10038 1 0039 1 0039 25 1 0038 1 0038 1 0039 1 0039 1 0040 1 0041 25 5 1 0039 1 0040 1 0040 1 0041 10041 1 0042 26 1 0040 1 0041 1 0042 10042 10043 1 0043 26 5 1 0042 1 0042 1 0043 1 0043 10044 1 0045 27 1 0043 1 0044 1 0044 10045 10045 1 0046 27 5 1 0044 1 0045 1 0046 1 0046 10047 1 0047 28 1 0046 1 0046 1 0047 10048 1 0048 1 0049 28 5 1 0047 1 0048 1 0048 1 0049 1 0050 1 0050 29 1 0049 1 0049 1 0050 10050 1 0051 1 0052 29 5 1 0050 1 0051 1 0051 1 0052 1 0052 1 0053 30 1 0052 1 0052 1 0053 1 0053 1 0054 1 0055 28 J USER S GUIDE Aprenvix 3 EVAPORATION LOSS Procedure for the determination of evaporation loss Use the same distilled water weighing vessel and balance as you will be using for the gravimetric check Half fill the weighing vessel with distilled water Cover the weighing vessel with its lid and place it on the balance using a pair of tweezers Aspirate a sample Tare the balance and take the weighing vessel out of the balance Take off the lid with tweezers Dispense the sample into a dummy vessel Replace the lid on the weighing vessel and using tweezers replace the vessel on the balance Read the neg
7. 807011 Piston assembly F807017 F807018 F807019 F807020 96 J USER S GUIDE prennix 1 EXAMPLE OF A PERFORMANCE CHECK Below is an example of how to evaluate the performance of PIPETMAN P10M at 1 uL 1 Determine the mean value e of the evapora 4 Calculate the mean volume tion loss e that occurs during your pipetting cycles Proceed as described in Appendix 3 V W e xZ to determine e V 0 964 0 018 x 1 0032 V 0 985 uL For a temperature of 21 5 C and an air pressure of 1013 hPa the Z factor is equal to 1 0032 uL mg see table in Appendix 2 m number of weighings 0 016 mg 3 0 021 mg 2 0 018 mg 4 0 017 mg 5 Evaluate accuracy e e1 e2 e3 e4 4 e 0 016 0 018 0 021 0 017 4 e 0 018 mg per cycle E V Vo Systematic error E Vo true value set on the instrument E 0 985 1 0 015 uL 2 Change the pipette tip and perform Relative a a ea tie ee y p g E E 0 015 x 100 1 1 50 measurements Wr 0 957 mg W1 0 968 mg Ws 0 966 mg W2 0 960 mg W7 0 955 mg W3 0 984 mg Ws 0 972 mg W4 0 942 mg Wo 0 958 mg Ws 0 969 mg Wi0 0 967 mg 6 Evaluate precision repeatability Standard Deviation SD Wr rinsing measurement which is disregarded for the calculation 3 Calculate the mean weight 0 968 0 964 0 960 0 964 0 984 0 964 Z 1 n W gt We e 2 1 01942 0964 0969 0964 0966 0964 9
8. If the pipette is very dirty a brush with soft plastic bristles may be used 4 Torinse wipe the entire pipette with a soft cloth or lint free tissue impregnated with distilled water 5 Leave to air dry Internal The following components only can be immersed in a cleaning solution tip ejector tip holder connecting nut and piston 1 Disassemble the pipette as described in Chapter 14 2 Set aside the handle in a dry and secure location 3 Clean the individual components using an ultrasonic bath 20 minutes at 50 C or witha soft cloth and brushes 4 Rinse the individual components with distilled water 5 Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue 6 Lubricate the piston and reassemble the pipette according to the instructions given in Chapter 14 ye I USER S GUIDE Decontamination Autoclaving However after separation from the body any of the Caution The body handle of following components of the volumetric module may be autoclaved individually tip ejector connecting nut tip holders pistons and seals except O rings the pipette is not autoclavable 1 Clean the parts to be autoclaved especially the tip holders 2 Put the parts in an autoclaving bag 3 Autoclave for 20 minutes at 121 C and 0 1 MPa 4 Check that the parts are dry before reassembling the pipette Caution The specifications of the pipette must be checked after changing
9. SASSEMBLY before you click to exit The software select DISASSEMBLY the Click to exit then resets the piston and the display returns to the last mode used following screen appears Now you can remove the lower part of your pipette with complete security b Service Info provides you with maintenance information you cannot modify any of the items e Number of pipetting cycles since last maintenance e Number of weeks since last maintenance c Service Settings gives you the option to organize your maintenance you can set following service points e Number of cycles until next maintenance e Number of weeks until next service e Service date e Activate or deactivate the Service alert MS Changes of service settings will reset all the service info to zero d Pipette Info provides information about your pipette you cannot modify any of the items e Version of firmware e Serial number e Total number of cycles one cycle up and down of the piston Number of readjustments carried out by this pipette Adjustment Press the mode button to enter the menu Select configuration by rotating the push button and click to confirm your selection Enter the sub menu in the same way and select the adjustment menu The adjustment menu allows you to access the following items e Standard Readjustment e Reset Settings Standard Readjustment This menu allows you to adjust the pipette using 3 calibration p
10. ZE YOUR PIPETMAN M Tip ejector Extension for use with P10M In orderto eject D10tips tip ejector extensions are supplied with P10M pipettes The tip ejector extension which is made of PVDF polyvinylidene Fluoride is autoclavable polioeLman A To fit a tip ejector extension e Hold the pipette with the push button upright e Hold the extension with the slot upright Slide the extension over the tip holder e Push the extension firmly onto the end of the tip ejector until it clicks into place see opposite To remove a tip ejector extension see opposite e Hold the pipette in one hand and grip the extension with the other e Gently twist the extension either direction and pull it away from the pipette Personal Label Name and or Application Tag Your PIPETMAN M offers you two options to mark your pipette You can personalize your pipette with a name tag and or an application tag Window Tag Clip tag e Remove the window by inserting a small screw driver in the access slot e Position the name tag on the pipette e Clip the window back in place Clip Tag Pull off the clip Window tag e Position the name tag into the clip e Clamp the clip back in place 1 1 GOOD LABORATORY PRACTICE FEATURES Your PIPETMAN Mis fully compliant to IS08655 standard and is CE marked for IVD and EMC directives PIPETMAN M includes the following GLP features General Lockable volume Useful volume
11. any part 5 Set the pipette aside to stabilize at room tempera ture for at least 6 hours Chemical Decontamination You may choose to decontaminate your pipette chemically in accordance with your own procedures Whatever decontaminant you use check that it is compatible with the plastics used in the construc tion of the pipette see page 22 Parts that may not be immersed 1 Wipe the handle of the pipette with a soft cloth or lint free tissue impregnated with the chosen decontaminant 2 Wipe the handle of the pipette with a soft cloth or lint free tissue impregnated with distilled water Parts that may be immersed The following components only can be immersed in a decontaminant solution tip ejector tip holder both parts connecting nut piston including holder and guide 1 Disassemble the pipette as described in Chapter 14 2 Immerse the components in the decontaminant solution or wipe them according the instructions given by the manufacturer or supplier of the decontaminant 3 Rinse the individual components with distilled or sterile water 4 Leave the parts to dry by evaporation or wipe them with a clean lint free tissue or soft cloth 5 Lubricate the piston and reassemble the pipette according to the instructions given in this chapter 93 DIDeELMOaN m Nf 1 6 LEAK TEST This test may be performed at any time to check that the pipette does not leak especially after performing a maintenance or decon
12. ative result e record the absolute value Repeat steps 3 to 8 three times to obtain e2 e3 and e4 a Pa ee SS YP NY SS 0 Calculate the evaporation loss e using the formula 1 FS n normal conditions this value is usually between 0 01 mg e e e e3 e4 and 0 03 mg Al 00 DIDeELMAaAN fi Plbetmani prennix 4 HOW TO CALCULATE VOLUMETRIC ACCURACY AND PRECISION Accuracy and precision are qualitative terms The corresponding quantitative terms are systematic error and random error Evaluation of accuracy The specified accuracy is the limit to the system atic error which is the difference between the mean volume of actual measurements and the true value of the volume set on the instrument The systematic error E can be estimated as follows E V Vo E systematic error Vo nominal volume V mean volume 1 n Sv Vi individually measured volume NM number of measurements The accuracy of a pipette can be expressed as a percentage of the nominal volume Important The mean value and number of replicates must be stated andthe experimental procedure used must be described in such a way that other workers can repeat it Evaluation of precision The specified precisionis the limitto the random error which is the distribution of the measured values around a mean value For pipettes preci sion refers to a within series group of data and theref
13. card the extra volume andcontinue dispensing the aliquots PIPETMANM distributes the aliquot volume each time you click the push button The number of aliquots left to dispense is displayed on the screen V vol HS A warning beep indicates the last aliquot 4 To purge Press the push button PURGE appears on the screen click again to purge and reset the piston Keep your thumb pressing the push button until the end of the purge START button LI Optional STOP A Discard the piston reset takes place after you _ amp Click to Purge Start Purge release the push button again This feature enables you to remove the tip from the liquid without aspirating anything 5 Eject tip by pressing the ejection button MIX Mode This is the classic pipette mode forward pipetting followed by a mixing phase composed of repeatedly aspirating and dispensing as well as an optional forward pipetting step tion oligonucleotide dilution and to mix samples with gel loading buffer and so on you can mix two different solutions or homogenize one solution If you work with higher viscosity than water e g restriction enzyme you may change the aspiration speed refer to chapter XX Ifyou work with genomic DNA you should always pipette very carefully to avoid shearing and nicking Application notes The Mode MIX can be used to prepare a PCR master mix enzyme restriction mix protein solu e Press Mode button to access menu where
14. e Battery Tags set of 4 4 Battery Tags F807013 4 Ejector Tags Ejector Tags set of 4 e Tip ejector extension P10M model only F807014 e User s Guide Please contact your Gilson Distributor for Quick Guide these accessories e Safety bag e Certificate of conformity including bar code sticker e Lubricant except for P10M 3 GETTING STARTED PIPETMAN Mis provided witha minimal battery charge Before using your new PIPETMANM westrongly recommend you to fully charge the battery PIPETMAN M charges 80 of its full battery capacity in less than an hour it takes three hours to fully charge the battery Please refer to PIPETMAN M Power Management Chapter 12 for charging procedure Volume range Switch on your PIPETMAN M Press the push button to activate the pipette The start up screen appears The pipette will do a self calibration test the firmware resets the piston Version of During initialization displayed on the screen you will see the volume firmare Ne range version of firmware and the number of readjustments R1 Your PIPETMAN Miis preset on PIPET mode and is now ready to pipette Number of readjustments see Chapter 5 PIPETMAN M goes into sleep mode after 3 minutes of inactivity The display will shut off Just press the push button to reactivate the pipette your last setting and pipetting mode will appear on the screen J USER S GUIDE 4 DESCRIPTION PIPETMAN M CQ
15. feeling that you are using a PIPETMAN P That s all you need to perform your pipetting tasks easily Mode button Push button set the volume by turning the button left or right and pipette with a simple push Mode button to access additional features like repetitive mode mix function or easy readjustment Push button PIPETMAN M is nothing less than a PIPETMAN and much more PIPETMAN M is available in 4 models covering a range from 0 5 uL to 1000 uL Easy to use only one push button for all pipetting functions More pipette techniques for a large number of applications standard pipetting repetition mixing and reverse Maximal comfort motorized aspiration and dispensing easy ejection of tips to avoid repetitive Strain injuries RSI Minimal effort PIPETMAN M is much lighter than other motorized pipettes RSI No user effect high reproducibility and accuracy due to motorized pipetting More fun for better performance Model Reference Range Color code P10M F81001 0 5 uL to 10 uL Red P20M F81002 2 uL to 20 uL Pale yellow P200M 81003 20 ul to 200 iL Yellow P1000M F81004 100 ul to 1000 pL Blue DIDELUMAN fi 2 PARTS CHECK LIST AND ACCESSORIES Contents Accessories Just take a moment to verify that the following The following items are available separately LeS are PrESEN Stand Adapter for CARROUSEL and SINGLE e PIPETMAN M pipette F807023 e Power Supply 5V with AC adapter and cabl
16. ffer into samples etc F e Press Mode button to access system menu where you can choose between different pipette modes Select REPETITIVE Mode by turning the push button To confirm your selection click on the push button e Turn the push button half way the screen will display click to change vol Click to e Now press the push button the volume on the display will start to blink you change volume can now adjust the volume e Set the aliquot volume click the push button set the volume by turning the push button and click again to confirm your settings e Set the aliquot number The maximal number of aliquots has been calculated by your PIPETMAN M The number of aliquots flashes and you can decrease this number N n 1 by turning the push button Click again to confirm your settings You are ready to pipette 1 Fita tip suitable for the model of PIPETMAN M that you are using preferably use PIPETMAN DIAMOND Tips for assur ance of accurate and precise results refer to chapter 8 Caution You must always fit a tip before using any pipette 2 Click push button to aspirate total volume The volume as pirated will bea little bit more than required extra volume The extra volume is necessary to ensure equal operating conditions for each dispensed aliquot DIDECLMOAl fi Miva op 3 To dispense click the push button DISCARD appears on the screen DISCARD ALIQUOTES V extra vol Dis
17. holder and tip ejector may change color however this has no impact on the performance Changing the seal and or O ring see Chapter 20 The O ring is positioned on the piston it should not be autoclaved if worn or damaged in any way it must be replaced To access the O ring remove the tip ejector and unscrew the lower part of the tip holder You should now be able to remove the O ring from the piston Sometimes the O ring may be found in the recess at the top end of the lower part of the tip holder If required lubricate the piston see How to Lubricate the Piston then fit a new O ring by sliding it onto the piston Reassemble the pipette Depending on the pipette model the dimensions of the O ring will vary refer to Chapter 20 Servicing the Piston Caution The specifications of the You may remove the piston assembly to clean lubri pipette must be checked after cate or change the piston changing any part I a Removing the Piston 1 Remove the tip ejector and optionally the lower part of the tip holder If you remove the lower part take care to remove the O ring as described in Changing the O ring Unscrew the connecting nut turn by hand coun terclockwise Gently remove the connecting nut and upper tip holder Pull off the piston assembly from the body of the pipette separate the parts see below Clean and autoclave if required the piston and holder together with any other part
18. humidity 50 e Barometric pressure 101 kPa e Use of distilled water grade 3 ISO 3696 e Tenmeasurements for each test volume which are Nominal Volume 50 of Nominal Volume and 10 of Nominal Volume Maximum Permissible Errors Model Volume Gilson ISO 8655 Reference Systematic Random Systematic Random error uL error uL error uL error uL P10M Min 0 5 0 04 lt 0 013 0 12 lt 0 08 F81001 1 0 025 lt 0 012 012 lt 0 08 5 0 060 lt 0 020 0 12 lt 0 08 Max 10 0 080 lt 0 025 0 12 lt 0 08 2 0 075 lt 0 025 10 0 100 lt 0 035 20 0 150 lt 0 05 P200M Min F81003 Max P1000M Min 100 2 50 F81004 500 3 50 Max 1000 5 00 1 9 SAFETY PRECAUTIONS AND LIMITATIONS OF USE For safety reasons it is important to observe the following instructions 1 Battery and electrical specifications We strongly recommend you fully charge the battery before using the pipette PIPETMAN M is supplied with an AC adaptor that is suitable for your country You must only use an original Gilson AC adaptor specific to this product Charge the battery in the pipette using the AC adapter or the stand adapter Use AC adaptor and stand adapter indoors PIPETMAN Muses Lithium lon battery Operation on the battery is done at user s risk only Opening the pipette voids the warranty Dispose of used batteries in accordance with legal regulations Batteries may not be disposed of with household waste and may e
19. ipette is out of adjustment Connecting nut is loose Pipette is not precise Unscrewed lower part of tip holder Incorrect operator technique Worn O ring Connecting nut is loose Damaged or corroded piston Damaged tip holder free of chemical biological or radioactive contamination Please use the included safety bag Tips fall or don t fit Low quality tips Damaged tip holder Damaged tip ejector No OLED display Pipette is in sleep mode Battery needs recharging Software needs to be reset No operation possible Battery needs recharging Software needs to be reset Calibration impossible Software needs to be reset 24 1 8 SPECIFICATIONS I USER S GUIDE PIPETMAN Mis a high quality pipette that offers excellent accuracy and precision it is fully compliant with ISO 8655 and is CE marked for IVD and EMC directives The figures given in the Gilson Maximum Permissible Errors table were obtained using PIPETMAN DIAMOND Tips These figures are only guaranteed when using Genuine PIPETMAN DIAMOND Tips Each pipette is inspected and validated by qualified techni cians according to the Gilson Quality System Gilson declares thatits manufactured pipettes comply withthe requirements of the ISO 8655 Standard by type testing The adjustment is carried out under strictly defined and monitored condi tions ISO 8655 6 e Basis of adjustment Ex e Reference temperature 20 C e Relative
20. iquid to be pipetted AS TEONA aleatinr mm seconds c When aspirating keep the tip at a constant depth below the surface of the liquid refer to Table P10M P20M P200M P1000M d Change the tip before aspirating a different liquid sample or reagent e Change the tip if a droplet remains at the end of the tip from the previous pipetting operation f Liquid should never enter the tip holder To prevent this e Never turn the pipette upside down e Never lay the pipette on its side when there is liquid in the tip s g The Gilson Stand Adapter see Chapter 2 is recommended for use with the CARROUSEL and SINGLE Pipette Holder to store your PIPETMAN M pipette in the vertical position h When pipetting liquids with temperatures different to the ambient temperature pre rinse the tip several times before use in order to reach equilibrium between the temperatures of the liquid and the pipette s dead volume i For volatile liquids you should saturate the dead volume by aspirating and dispensing the liquid repeatedly before aspirating the sample j After pipetting acids or other corrosive liquids that emit vapors clean the pipette as described in Chapter 15 k The pipette can be used between 4 Cand 40 C but the specifications may vary see Chapter 18 o Caution Extreme temperatures Do not pipette liquids having temperatures above canaffectaccuracyandprecision 50 C or below 4 C 1 0 PERSONALI
21. iration speed is blinking set the speed by turning the push button and click to confirm your selection Set DISPENSING SPEED dispensing speed is blinking set the speed by turning the push button and click to confirm your selection After selecting the speed you go automatically back to pipetting mode 8 PIPETMAN DIAMOND TIPS PIPETMAN DIAMOND Tips are made to the highest specifications strict quality control is maintained throughout the manufacturing process These tips are used to calibrate PIPETMAN M therefore for optimum performance we recommend using PIPETMAN DIAMOND Tips with your PIPETMAN M However PIPETMAN M also offers you a high compatibility with a large Gilson logo ID Number number of other tips For more information please feel free to contact your Gilson Distributor PIPETMAN DIAMOND Tips have the Gilson logo engraved on their collar ensuring that you have a genuine Gilson product To ensure accuracy and precision Gilson s Quality Assurance System focuses on the following criti cal parameters PIPETMAN DIAMOND Tips are made from pure polypropylene virgin metal free to avoid the pos sibility of contamination They are available sterilized and with filters Sterilized PIPETMAN DIAMOND Tips are certified free of detectable RNases DNases DNA RNA and proteases Optimized shape revised collar for optimum sealing thin walls and fine point making them easier to mount more flexible with no vortexing a
22. nce Specifications subject to change without notifications errors omitted COs GILSON Gilson Inc World Headquarters 3000 Parmenter Street PO Box 620027 Middleton WI 53562 0027 USA Tel 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 Gilson S A S 19 avenue des Entrepreneurs BP 145 F 95400 Villiers le Bel France Tel 33 1 34 29 50 00 Fax 33 1 34 29 50 20
23. nd barometric pressure see Appendix II For volumes greater than 20 uL the evaporation factor can be disregarded For more information please refer to Verification Procedure for Accuracy and Precision which you can download from the Gilson website www gilson com Maintenance operations disassembly of your PIPETMAN M to change and clean parts It is best to inspect your pipette regularly and to routinely clean and change parts as required To help you to keep up a regular schedule and in the interests of GLP you can configure your pipette to display an alarm before servicing is due refer to Service Set tings page 18 Caution The following mainte nance operation should only be done when the pipette is in Mode DISASSEMBLY see page 18 Maintenance Warning gm Service pipet PIPETMAN M notifies you when maintenance is due service ee ad limit passed alert is set by default to ON The following message will appear after validation by a click the following message appears a ee _ l l l Click If you click to confirm within 10 sec the pipette will automati to service cally switch to disassembly mode so you can carry out your maintenance safely Service one Wait longer than 10 seconds and the option to have i l a reminder in one week will appear ______ gt Clickto confirm Your pipette will be operational again Tip Holder and Tip ejector These parts mus
24. nd improved precision PIPETMAN DIAMOND Tipsare free from even microscopic defects especially at the orifice All surfaces are smooth and hydrophobic thereby avoiding the excessive retention of liquids that causes poor accuracy and a lack of precision Mold and cavity references are marked on the collar ensuring the traceability For quality assurance purposes batch numbers appear on all packages bags and boxes etfs DIDELMOaN m Nf e They form an air tight seal with the tip holder preventing Caution Donot autoclave the leaks that cause poor accuracy and a lack of precision PIPETMAN DIAMOND PIPETMAN DIAMOND Tips except filter tips may be auto filter tips they have been claved at 121 C for 20 minutes at 0 1 MPa sterilized PIPETMAN DIAMOND Tips to use for best results GroP To ensure the best performance Pipette model Tips model Range Color code from your Gilson pipette you shoud ALWAYS use PIPETMAN DIAMOND b E Tips in accordance with IS08655 i i E l a to complete your pipetting system j because DIAMOND Tips were used to 0 1 10 uL establish the specifications 2 200uL Pale yellow 2 30 uL 2 200 ul Yellow 10 100 uL P1000M D1000 100 1000 uL D1200 100 1200 uL DF1000 100 1000 pL DF1200 100 1200 pL 9 GUIDELINES FOR GOOD PIPETTING Aspirate and Dispense 1 Fit new PIPETMAN DIAMOND Tip for the best results see Chapter 18 Push the tip holder into the tip using a sligh
25. ny excess remember only a fine film of lubricant is required over the entire piston 29 DICeLMOAN m ae 1 5 CLEANING AND DECONTAMINATION PIPETMAN M is designed so that the parts normally in contact with contaminants can easily be cleaned and decontaminated As part of your quality system you may have procedures Caution Liquidmustnotenterthe for pipette decontamination We recommend that you refer to Decontamination Procedures for Gilson Pi pettes which you can download from Gilson s website handle of the pipette If you use chemical solutions for decontamination or detergents for cleaning other than those speci fied below you should check with your supplier that the solution or detergent is safe for use with one of the following materials PP Polypropylene PBT Polybutylene Terephthalate PC Polycarbonate POM Polyoxymethylene PVDF Polyvinylidene Fluoride PEI Polyetherimide and stainless steel Cleaning The pipette must be cleaned as described below before it is decontaminated For cleaning your PIPETMAN M you may usea simple soap solution or any of the solutions mentioned in Decontamination Procedures for Gilson Pipettes External 1 Remove the tip ejector for cleaning see Chapter 14 2 Replace the elastomer plug to protect battery charging port 3 Wipe the entire pipette with a soft cloth or lint free tissue impregnated with soap solution to remove all dirty marks
26. of accurate and precise results refer to chapter 8 using any pipette To aspirate VOLUME 1 press the push button To dispense Click press and release the push button Three things happen sample dispense automatic purge and piston reset to zero or gt Keep your thumb pressing the push button until the end of the dispense cycle The piston reset takes place after you release the push button again This feature enables you to remove the tip from the liquid without aspirating anything To aspirate and dispense the second volume VOLUME 2 repeat steps 2 and 3 To mix Press the push button as long as you keep your thumb pressing the button the pipette continues mixing Release the push button to complete the current mixing cycle To purge press push button PURGE appears on the screen click again to purge and reset the piston To pipette If you have chosen a VOLUME 3 gt 0 you can now aspirate and dispense this volume by repeating steps 2 and 3 WS you choose VOLUME 0 step 2 won t occur if you choose VOLUME 2 0 uL step 4 won t occur as well if you set VOLUME 1 and 2 O uL step 1 4 will disappear If you set VOLUME 3 0 uL step 7 will not occur Push button pressed START button C Optional STOP 6 Start Purge boioetman m MESME REVERSE Mode The REVERSE Mode is like reverse mode pipetting with a manual pipette Dur Mode ing aspiration an amount of liquid is added After deli
27. oints 10 50 and 100 of nominal volume in according with ISO8655 recommendations You may want to calibrate your pipette for solutions with a density viscosity surface tension or vapour pressure that are different to that of water To return to the factory settings choose Reset Settings your PIPETMAN M will be reset to the three factory calibration values which are permanently stored in the pipette s firmware 18 J USER S GUIDE In accordance with ISO 8655 Gilson recommends a gravimetric procedure for pipette calibration This gravimetric method is used to establish the mean mass of a given volume of water taking into account evaporation losses where necessary After converting the mean mass to a volume using the Z factor see appendix Il enter the measured volumes s into the pipette s memory and the software readjusts the pipette accordingly This method requires the strict monitoring of environmental conditions and the use of routinely controlled equipment that is adapted to the volume being measured Conversion to volume must take into account the density of the liquid as well as evaporation during the cycle time For each measurement the corresponding volume Vi can be calculated as follows Vi Wi e Z Wi is the weight as read on the balance e is the mean evaporation loss during the cycle time Z expressed in L mg is a conversion factor incorporating density of water buoyed in air at test temperature a
28. ological chemical and or radiological contamination so as to protect from health hazards the persons involved SS in the disposal and recycling of equipment For more information about where you can drop off your waste equipment for recycling please contact your local dealer from whom you originally purchased the product or your local council By doing so you will help conserve natural Description PIOM P20M P200M P1000M resources and you will ensure that your Connecting nut F807012 F807012 F807012 F807012 waste equipment is recycled in a man Battery tag 1x4 F807013 F807013 F807013 F807013 ner that protects human health and the Ejector tag 1x4 F807014 F807014 F807014 F807014 environment Thank you Powersupply USB F807015 F807015 F807015 F807015 9 PIPETMAN M can be used indoor and USB cable F807016 F807016 F807016 F807016 outdoor Power tub cover F807022 807022 F807022 F807022 if precautions are respected as described Battery window F807005 F807005 F807005 F807005 above in this handbook and in the GLP Ejector window F807006 F807006 F807006 F807006 Lubricant tube 3 5g F3070902 F3070902 F3070902 Seal 1x5 F161902 Seal amp O ring 5 sets F144863 2 0 REPLACEMENT PARTS O ring 1x5 F2070501 F2070601 Tip holder upper part F2070117 F2070117 F2070517 F2070617 Tip holder lower part 2070218 2070318 F2070518 F2070618 Button assembly F807001 F807002 F807003 F807004 Tip ejector assembly F807008 F807009 F807010 F
29. ore to repeatability The random error is then quantified by the stan dard deviation of measurements performed ata given volume setting underthe same measuring conditions The standard deviation SD or s can be esti mated as follows V mean volume 1 n V re i Vi individually measured volume NM number of measurements minimum 10 The precision of a pipette can also be ex pressed as a percentage of the mean volume This is known as relative standard deviation RSD or coefficient of variation CV and is es timated as follows SD RSD x 100 V J USER S GUIDE E C DECLARATION OF CONFORMITY GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 VILLIERS LE BEL FRANCE Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 The company PIPETMAN M Hereby certifies on its sole responsibility that the P10M P20M P200M P1000M products listed below 98 79 EC comply with the requirements of the following In Vitro Diagnostic Medical Devices European Directives 2004 108 EC Electromagnetic Compatibility EMC 2006 95 EC Low Voltage Directive LVD Annex lll self declared Villiers le Bel September 1st 2009 Atika El Sayed Herve Ledorze General Manager Quality Manager y r CE 4 GILSON 31 www gilson com sales gilson com service gilson com training gilson com LT801527 D 2010 Gilson SAS All rights reserved August 2010 English Printed in Fra
30. range is printed on the push button Volume range is displayed on the screen Serial number is engraved on the body handle of the pipette and encoded in the firmware Bar code on the box and with the certificate can be transferred Personalization by nametags for marking application and or user name see Chapter 10 14 J USER S GUIDE Cycle counters From last volume setting to count the number of cycles in the current run From manufacture and since the last service see Chapter 14 e By indicators R1 R2 R3 Rx each time pipette is readjusted e Maintenance intervals can be specified by weeks or number of cycles elapsed Alarms e Low battery warning e Service is overdue warning see Chapter 14 battery low e Service Alert can be set On or Off 1 2 PIPETMAN M POWER MANAGMENT The PIPETMAN M battery has been designed to ensure 800 pipetting cycles at maximum speed upto seven 96 well plates between two charge cycles For service continuity when needing more capacity your PIPETMAN M can also be used while charging with the same performance as a fully charged pipette Battery Charging PIPETMAN M charges 80 of its full battery capacity in less than an hour it takes three hours to fully charge the battery 1 Charge your pipette by using the power supply Connect the AC adapter to a suitable AC power supply Plug the adapter into the USB port of the pipette The pipet
31. s later and will continue charging Changing the battery If the following symptoms occur please contact your Gilson Service Center for battery change MN Caution PIPETMAN M uses Lithium lon battery Operation on the battery is done at user s risk only Opening the pipette voids the warranty PIPETMAN M battery won t charge or will not operate even if connected to the charger at least for 10 minutes PIPETMAN M battery provides you very short cycle time or permanently needs to be used in connected mode Mode button 1 3 CONFIGURATION Enter the menu using the Mode button You will find not only the pi petting programs PIPET REPETITIVE MIX and REVERSE and the speed control but also the menu CONFIGURATION including SERVICE and ADJUSTMENT refer to Chapter 14 The navigation of the menus is always done in the same way gt Make your selection by rotating the push button enter and confirm with a simple click Push button J USER S GUIDE The configuration menu allows you to set up following items 1 Volume limit Here you can fix the maximal volume of the pipette by default nominal volume For example you have a P200M pipette but you would like to use it with your PIPETMAN DIAMOND Filter tips DF 100 you can set the maximal volume at 100 uL and you won t risk contamination of your pipette 2 Beeper You can switch the tone of your pipette on or off 3 Contrast You can choo
32. s that may need to be treated in the same way see Chapter 15 RS n the case of P1000M the piston holder piston and guide are a unit Don t try to separate them Lubricate the piston see below Reassemble the piston and guide then carefully insert the assembly into the body of the pipette The guide should hold the piston assembly inside the body of the pipette You can hear a click when the piston is back in place HS Jakecarenotto touch the piston and thatitis dust free If needed clean the assembly with a dust free cloth Reassemble the upper part of the tip holder and the connecting nut and then refit to the body of the pipette by rotating the connecting nut clockwise until it is finger tight Fit the O ring for P10M the seal and reassemble the lower part of the tip holder Refit the tip ejector J USER S GUIDE Caution Don t pull on the piston to remove the assembly a holder lower ae nut Tip holder upper Piston holder Piston aa Guide Connecting nut The seals for P10M are fragile and can be used only once So after unscrewing the lower part of the tip holder you must fit a new seal How to Lubricate the Piston except P10M Use only Gilson lubricant ref F2070902 as supplied Squeeze a small quantity from the tube onto a clean lintless cloth Use the cloth to transfer the lubricant to the piston Ensure that the piston is evenly lubricated and that you wipe away a
33. se between values of 1 to 5 to set up the contrast of the screen 4 Service see Chapter 14 5 Adjustment see Chapter 14 1 4 MAINTENANCE OF YOUR PIPETMAN M PIPETMAN M requires very little maintenance However to ensure pipette accuracy precision and robustness for timeless use please proceed periodically with a Two minute Inspection as recom mended by Gilson Your PIPETMAN M allows you to e Get service information e Readjust the pipette to user settings e Return to factory settings e Replace spare parts for detailed information see Chapters 14 and 19 e Prepare the pipette for cleaning or autoclaving by disassembly of the parts specified see Chapter 15 Service Information PIPETMAN M provides you with all required service information It will help you establish an easy diagnosis and plan any service operation with your accredited Gilson Service Center You will find in the service menu following sub menus a Disassembly b Service Info c Service Settings d Pipette Info The navigation of the menus is always done in the same way gt Make your selection by rotating the push button enter and confirm with a simple click DiIDeLMan i Mike ep a Disassembly This option allows you to disassemble the lower part safely protection of the pis ton and the motor If you Caution You must first ensure that the pipette has been correctly reas sembled see User Maintenance DI
34. t be changed if they are damaged You may also remove these parts for cleaning or decontamination purposes Changing the Tip ejector 1 Keep the tip ejector button depressed and grip the top of the tip ejector with the other hand 19 pioetman ii MIR ep 2 Gently rotate the tip ejector counter clockwise and separate its connector from the operating rod 3 Pull the tip ejector away from the body of the pipette 4 Clean or autoclave the tip ejector and refit or replace it by reversing the procedure Changing the Tip holder lower part After removing the tip ejector you may remove the lower part of the tip holder which is more likely to become contaminated or damaged than the upper part Removal of the lower part is shown below for the upper part see Removing the Piston special precautions are necessary 1 Gently rotate the lower part of the tip holder counterclockwise to unscrew it from the upper part 2 Separate the parts and remove the O ring see Changing O ring 3 Clean and if required autoclave the lower part of fm ip holder upper the tip holder autoclaving 20 min at 121 C and 0 1MPa 4 If required lubricate the piston see How to Lubricate the Piston and fit a new O ring 5 Screw the two parts together making sure that P Telder lower oy the two parts are fully tightened by hand 6 Refit the tip ejector Piston FS After autoclaving the tip
35. t twisting motion to ensure a firm and airtight seal 2 Pre rinse the tip Pre rinsing consists of aspirating the first volume of liquid and then dispensing it back into the same vessel or to waste Subsequent volumes that you pipette will have levels of accuracy and precision within specifications Some liquids e g protein containing solutions and organic solvents can leave a film of liquid on the inside the wall of the tip pre rinsing the tip minimizes any errors that may be related to this phenomenon 3 Hold the pipette vertically and immerse the tip in the liquid see page 13 Press the push button to aspirate the set volume of liquid Wait a few seconds time depends on model see page 13 then withdraw the pipette tip from the liquid You may wipe any droplets away from the outside of the tip using a medical wipe however if you do so take care to avoid touching the tip s orifice 4 Place the end of the tip against the inside wall of the recipient vessel at an angle of 10 to 40 Press the push button Wait for at least a few seconds before releasing the push button to expel any residual liquid from the tip While removing the pipette draw the tip along the inside surface of the vessel 5 Eject the tip by pressing firmly on the tip ejector button a 22 I USER S GUIDE General Guidelines for Good Pipetting a Make sure that you fit new tips Immersion Depth and Wait Time b Eachnewtip should be pre rinsed with the l
36. tamination procedure If a pipette fails this test you should replace the faulty part e g O ring tip holder and repeat this test after making sure that the pipette is correctly reassembled e Fit a PIPETMAN DIAMOND Tip e Set the pipette to the nominal volume e Aspirate the nominal volume from a beaker of distilled water e Hold the pipette in the vertical position and wait for 20 seconds e Ifa water droplet appears at the end of the tip there is a leak see Chapter 17 e If you see no droplet re immerse the tip below the surface of water e The water level inside the tip should remain constant if the level goes down there is a leak see Chapter 17 Problem Possible Cause Pipette is leaking sample Worn O ring 1 7 Pipette won t aspirate Worn O ring TROUBLESHOOTING Unscrewed lower part of tip holder Damaged or corroded piston Incase of malfunction first reset the pipette by Damaged tip holder Improper repair or assembly Connecting nut is loose Software needs to be reset pressing on the push button and the Mode button simultaneously at least for 8 seconds If the problem persists you may consult the Noisy operation Piston needs lubricating following table which identifies potential problems and their solutions Caution Before returning any pipette to yourlocal Gilson Service Center ensure thatitis completely Pipette is inaccurate Improper repair or assembly Unscrewed lower part of tip holder P
37. te starts charging 2 Charge your pipette by using stand adapter see Chapter 2 Firstplacethestandadapter on your Gilson SINGLE pipette holder or CARROUSEL Remove the plug Now place your pipette on the stand adapter and plug the power Stand adapter Caution Use only the original power transformer supplied by Gilson Use of an incompatible power supply can transformer into the stand adapter damage your PIPETMAN M The pipette starts charging DIDELMOaN m Nf Working with charger connected to the pipette PIPETMAN M can also be used for pipetting while charging Plug the power supply into your pipette Press the push button to start your pipette PIPETMAN M is ready to pipette w You can also recharge PIPETMAN M by USB with yourcomputer howeverit willtake much longerthan by power supply N Caution Never work with PIPETMAN M while charging by computer Low Battery Warning PIPETMAN M has a low battery alert As the battery runs down the LOW BATTERY message appears The warning is replaced bya blinking symbol Hattern low of an empty battery If it is not recharged the pipette will switch off after a while Settings will not be lost If the battery is completely discharged black screen no operation please connect the pipette to the charger for at least five minutes then a batt too low message will appear The pipette will display the last settings used a few minute
38. urate and precise results refer to Chapter 8 using any pipette 2 Clickpush button to aspirate the selected volume Anamountof liquid equaltothe amount of purged V extra vol air is added the volume aspirated will be a little bit more than the set volume 3 Click push button to dispense the volume the additionally amount of liquid remains in the tip 4 To purge press push button PURGE appears on the screen click again to purge and reset the pis ton Keep your thumb pressing the push button until the end of the purge the piston reset takes place after you release the push button again This feature enables you to remove the tip from the liquid without aspirating anything START button X Click to Purge 5 Eject tip by pressing the ejection button Optional sr Start Purge lt 10 I USER S GUIDE 7 PIPETTING SPEED CONTROL PIPETMAN Mis set by default on speed 6 You may need to change the speed of aspiration or dispensing depending on your application You can change aspiration speed and dispensing speed independently of one other from very slow to very fast speed 1 speed 6 Your speed selection is memorized for each pipetting mode until you change it again Press Mode button to access system menu where you can find the different pipette modes the speed menu and configuration menu Select SPEED by turning the push button to confirm your selection click on the push button Set ASPIRATION SPEED asp
39. ush button half way the screen will display click to change vol Click to e Now press the push button the volume on the display will start blinking you can now adjust the volume e Turn the push button either clockwise to decrease volume or counter clockwise to increase volume like indicated on the button e Press the push button one time when finished The volume is locked change volume You are ready to pipette PIPET Mode This is the classic pipette mode forward pipetting your pipette is simply aspirating and dispensing A Application note You can use the PIPET Mode for all standard applications like DNA extraction Plasmid Isolation Cloning Dilution PCR qPCR and many others You can easily pipette aqueous liquids like buffer chemical solutions MgCl2 KCI and biological samples like blood DNA and RNA e Fit a tip suitable for the model of PIPETMAN M that you are Caution You must using preferably use PIPETMAN DIAMOND Tips for assurance always fit a tip before of accurate and precise results refer to Chapter 8 using any pipette e Press the push button to aspirate the selected volume e To dispense Click press and release the push button Three things happen sample dispense automatic purge and piston reset to zero or Keep your thumb pressing the push button until the end of the dispense cycle The piston reset takes place after releasing the push button again This feature enables
40. very the excess volume remains in the tip and is discarded REVERSE a Application notes The REVERSE Mode is ideal for pipetting viscous liquids liquids with high vapour pressure or E those which tend to foam Your PIPETMAN M aspirates a selected volumeand an excess This excess compensates for the liquid that remains as a film inside the tip during si For example protein extraction cell lysis plasmid isolation cell culture buffer preparation and many others Electrophoresis gels loading protocol First change the pipetting speed of your pipette see chapter X Aspiration can be done by using the standard speed by default speed 6 Dispensing should be done very slowly and carefully to prevent swirling and spilling of the samples so select the lowest speed 1 Your pipette is now ready to load your gel Please be aware that if you purge without pulling out the tip air bubbles could get into your gel e Press Mode button to access system menu where you can choose between different pipette modes Select REVERSE Mode by turning the push button to confirm your selection click the push button e Set the volume click the push button set the volume by turning the push button and click again to confirm your settings You are ready to do reverse pipetting 1 Fit a tip suitable for the model of PIPETMAN M that you are Caution You must using preferably use PIPETMAN DIAMOND Tips for assur always fit a tip before ance of acc
41. xplode if disposed in fire e Li ion battery pack 1 Ah 3 6 V Charging time approx 3 hours for a fully discharged battery 80 in one hour e AC adaptor Input voltage country specific 100 240 V 50 60 Hz 0 5A max Output voltage 5 V DC 3 5A 17 5W max e Class IIL this device is double insulated 25 oioebmMan amp MIR ep 2 Do not use PIPETMAN M ina potentially explosive environment or with potentially explosive chemicals 3 When pipetting infectious radioactive toxic and other hazardous solutions please observe all the safety precautions e g wear protective clothing goggles and gloves and regulations appropriate for your country 4 Donotallow the liquid to enter the body of the pipette Storage conditions Temperature 20 C to 50 C Humidity max 80 6 Temperature of use Between 4 C and 40 C specifications may vary 7 Use only original Gilson s DIAMOND tips accessories and spare parts If the equipment is used in a manner not specified by Gilson in the user s guide the protection provided in the equipment may be impaired 8 Equipment disposal This equipment must not be disposed of with unsorted municipal waste Instead it is your responsibility to correctly dispose of your waste equipment by handing it over to an authorised facility for separate collection and recycling It is also your responsibility to decontaminate the equipment incase of bi
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