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Sampling and Sample Culture

1. Capacity ml Dimensions mm Pk Cat No 120 716x178 500 129 9850 540 114x229 500 129 9851 810 114x305 500 129 9852 720 140x229 500 129 9854 1080 114x382 500 129 9853 1260 140x382 500 129 9855 1650 178x305 250 129 9856 e 1800 254x305 250 129 0012 3000 254x382 250 129 0013 e Flat regular wire with write on panel Capacity ml Dimensions mm Pk Cat No 120 16x178 500 129 9859 X e 720 140x229 500 129 9862 810 114x305 500 129 9861 1650 178x305 250 129 9863 g 1800 254x305 250 129 0010 g 3000 254x382 250 129 0011 g Pressure Seal Bags Sample Bags Nalgene Sample Bags Minigrip with Kangaroo pocket 300 9 Resealable pressure seal bag for storing and protecting food and all kinds of small parts and spare parts e Can be resealed repeatedly flexible and tear proof e Suitable for use with food e Transparent material means contents are clearly visible e Simple sealing with guide tracks on the pressure seal Description Pk Cat No LDPE 80x120 mm 100 129 9142 g LDPE 100x150 mm 100 129 9143 LDPE 120x170 mm 100 129 9144 LDPE 160x220 mm 100 129 9145 e LDPE 180x250 mm 100 129 9146 LDPE 300x400 mm 100 129 9147 PE 200x300 mm 100 129 9136 G PE 250x350 mm 100 129 9137 HDPE 220x310 mm 100 129 9154 LDPE Ideal for small lab items botanical samples and other specimens Not recommended
2. Height Version mm mm Sterile Pk Cat No Contact dish 65 14 5 720 391 1504 5 Feed 90 76 2 700 391 1518 5 High with one vent o9q 76 2 700 391 1521 5 High with triple 30 16 2 700 391 1517 5 vents 90 16 2 700 391 1520 7 rar 30 16 2 700 391 1519 5 High without vents 90 162 7 700 391 1522 55 74 2 z 1620 391 0865 5 55 74 2 1620 391 0895 lt J0 74 2 z 600 391 0875 5 oe 90 74 2 F 600 391 0878 5 With triple vents J0 74 2 z 825 391 0891 5 30 14 2 825 391 0892 5 740 20 6 776 391 1500 5 140 20 6 7 176 391 1502 5 90 74 2 600 391 0877 5 90 74 2 600 391 0880 5 55 T42 1620 391 0866 5 55 142 1620 391 0868 5 Without vents 90 74 2 a 825 391 0893 5 30 74 2 825 391 0804 5 740 20 6 176 391 1501 5 740 206 176 391 1503 5 With vents 90 14 2 E 825 391 1546 Petri Dishes Star Dish Phoenix For simple colony counting e Stackable e Patented ribs guarantee stability during casting e Venting system avoids condensation Height Version mm mm Pk Cat No Cae eee 40 12 5 720 710 3504 Hi 47 12 5 750 710 3502 Without vents 55 15 600 710 3513 With 4 vents 55 15 600 710 3512 With 3 vents 85 15 600 710 3511 Stackable with 4 vents 90 15 600 710 3505 With 4 compartments 90 15 600 710 3519 g Stackable without vents 90 15 600 710 3506 Stackable with vents 90 15 600 710 3517 Stackable with 4 vents 90 15 60
3. a A g Maximum food safety with Merck foodproof real time PCR kits See Microbiology section Chapter 7 391 10 VWR F www vwr com Cell Culture Innoculating Needles and Accessories Sterile disposable plastic e Moderately flexible e Colour coded plastic Inoculating Needles Description Colour Packed Pk Cat No Needle green peel pouch 1000 632 0056 green 20 bag 1000 632 0057 P a Spreaders VWRES Convenient and economical these cell spreaders are designed to simplify bacterial culture work e Durable enough to be autoclaved and used again Description Colour Packed Pk Cat No Sterile blue Individually wrapped 25 612 2688 7 yellow Individually wrapped 25 612 2690 e blue Bulk 25 612 2687 e Mon Steril yellow Bulk 25 612 2689 z oan Spreaders L shaped VWRES The foot of the spreader has a completely smooth rounded surface free of rough edges and imperfections It enables even spreading of liquid samples across the surface of agar plates without gouging or cutting the medium see Surface treated to enhance adhesion of droplets e Non toxic Gamma irradiated Dimensions 135x35 mm Description Colour Packed Pk Cat No blue individually packed 500 612 1560 Spreaders L shaped blue bag of 5 1000 612 1561 5 Tel 0800 22 33 44 freephone Email uksales uk vwr com 391 11 Fax 01455 55 85 86 Web http uk vwr com Cell Cult
4. Formulation Frozen in 15 mM acetic acid Source Bovine articular cartilage Quality control Quality controlled by SDS PAGE and tested and found negative for bacteria fungi and mycoplasma Storage and stability Stable for at least three months at 70 C Description Pk Cat No BD Collagen II bovine 5 mg 734 1272 392 59 VWR 3 j www vwr com Cell Culture Cell Culture Media and Supplements BD Collagen V BD Biosciences Collagen V is found in whole placenta amnion chorion and cornea It can be used as a thin coating on tissue culture surfaces to study Collagen V effects on cell behavior Collagen V has been shown to inhibit endothelial cell proliferation selectively Formulation Frozen in 10 mM acetic acid Source Human placenta Quality control Quality controlled by SDS PAGE and tested and found negative for bacteria fungi and mycoplasma Source material tested for hepatitis B antigen and HIV 1 antibody Storage and stability Stable for at least three months at 70 C Description Pk Cat No BD Collagen V human 250 uG 734 0106 BD Fibronectin BD Biosciences Fibronectin FN is found in interstitial matrix and plasma The principal function of Fibronectin appears to be in cellular migration during wound healing and development It can be used as a thin coating on tissue culture surfaces to promote attachment spreading and proliferation of a v
5. Description volume ml Growth area cm Pk Cat No 1 Chamber configuration 2 0 4 5 8 6 16 734 2055 2 Chamber configuration 1 0 2 0 4 0 16 734 2057 4 Chamber configuration 0 5 1 0 17 16 734 2059 8 Chamber configuration 0 2 0 5 0 7 16 734 2061 Flasks on Slides Nunc Glass Flaskette PS SlideFlask sterile For cell culture directly on a microscope slide Ideal for karyotyping of cells single cell autoradiography and single cell immuno fluorescence e Glass flaskette CE marked e PS SlideFlask Nunclon certified e SlideFlask is ultrasonically welded to the slide and individually leak tested Dimensions Flaskette 20x52 mm SlideFlask 18x50 mm Recommended working Description volume ml Growth area cm Pk Cat No Flaskette glass 2 5 5 0 10 16 732 2609 SlideFlask PS 2 5 5 0 9 50 734 2107 392 27 VWRe F www vwr com Coverslips Thermanox Nunc normal use e Resistant to commonly used solvents e Autofluorescent in the range 380 to 545 nm Safe easy to handle coverslips 0 2 mm thickness which remain flat during Cell Culture Cell Culture Chamber Slides e Surfaced treatment on one side for optimal cell attachment and growth Description Pk Cat No Rectangular coverslips 10 5x22 mm for 8 well multidish 500 734 2115 Rectangular coverslips 22x60 mm for 4 well multidish 500 734 2116 Rectangular coverslips 24x30 mm 500 734 2154 Ro
6. Sampling Samplers Solid Samplers Single Use Disposable Suitable for all industries pharmaceutical food chemical cosmetic biotechnology etc Pre sterilised versions available Length 1000 or 500 mm diameter 21 mm e Never have to be cleaned e Cross contamination risk is eliminated e Assembled in a cleanroom environment e Economical save time and money Packaging Each sampler is individually bagged 20 box Disposable PowderThief for sampling powders and granules Description Sterile Pk Cat No 2 202 HDPE 500 mm tip size 1 ml 9 200 2028 20 300 2029 2 2 HDPE 500 mm tip size 5 ml 0 30072030 20 300 2031 A 2 201 HDPE 500 mm tip size 10 ml 9 300 2018 20 300 2019 2 202 HDPE 500 mm tip size 20 ml 9 300 2020 20 300 2021 20 2022 HDPE 1000 mm tip size 1 ml 300720 20 300 2023 2 2024 HDPE 1000 mm tip size 5 ml 0 300720 20 300 2025 20 201 HDPE 1000 mm tip size 10 ml 20072016 20 300 2017 20 202 HDPE 1000 mm tip size 20 ml 20072026 20 300 2027 Disposable LiquiThief for sampling low viscosity liquids eg water Description for volume ml Sterile Pk Cat No z 2 2002 HDPE 500 mm 100 2 200200 20 300 2003 PP 500 mm 100 20 300 2005 F 2 2 HDPE 1000 mm 190 9 20072000 20 300 2001 PP 1000 mm 190 20 300 2004 Disposable ViscoThief for sampling creams and gel
7. The VWR e newsletter Register now at http eu vwr com e newsletter Tel 0800 22 33 44 freephone Email uksales uk vwr com 390 7 Fax 01455 55 85 86 Web http uk vwr com i Cell Culture Incubators Incubators Roll In Standard and CO3 Wheaton Temperature range ambient 8 to 70 C volume 1132 litres These incubators are an ideal space saving and economical alternative to a warm room for small production runs Designed specifically for use with the Wheaton roller culture apparatus they can also be used in combination with optional shelves to accomdate spinner flasks rocker tables and other equipment e Forced air circulation with digital temperature control gives temperature stability of 0 5 C at 37 C without shelves e Units have a viewing window in the door and four internal electrical outlets for shakers stirrers etc e CO9 incubator has light weight lifting ramps for rolling in roller culture apparatus e CO9 range 0 20 gas shuts off when door is opened or switch is turned off Design Units have a painted steel exterior the standard models have a painted WAN ij steel interior and CO models have stainless steel interiors Their double walled j ee construction provides temperature stablity and is easy to clean i _ Shelves Supplied without shelves which are available separately units can accommodate a maximum of 12 shelves Cleaning Inner chamber is easy to clean
8. Borosilicate glass This plate is used in the pharmaceutical or food processing industry and also in hospital environments for surfaces such as skin operating tables and work surfaces e Allow even distribution of culture media e Distortion free transparency e Resistant against temperature and chemicals Version mm Height mm Pk Cat No 54 5 20 1 391 0810 e 74 20 1 391 0820 e 94 15 1 391 0830 e Duroplan 37 20 T 391 0840 z 114 20 1 391 0850 143 26 5 1 391 0860 Petri dishes Soda lime glass e Bottom and lid flat inside and out e A cost effective alternative Version mm Height mm Pk Cat No 60 15 1 391 2710 e 80 15 1 391 2720 100 15 1 391 2730 100 20 1 391 2740 120 20 1 391 2750 150 29 1 391 2760 180 30 1 391 2770 200 30 1 391 2780 Petri Dish Flint glass e Can be autoclaved at a temperature of 120 C Version mm Height mm Pk Cat No Petri dish flint glass 100 15 18 391 2256 e Petri Dishes Pyrex borosilicate glass Complete with lid e Chemically resistant e Specially formed to ensure even wall thickness and consistent optical performance e Will withstand repeated autoclaving Version mm Height mm Pk Cat No 60 20 10 391 2023 80 20 10 391 2024 Petri dish glass 100 20 10 391 2025 gt 120 20 10 391 2026 150 20 10 391 2027 Tel 0800 22 33 44 freephone Email u
9. Description Pk Cat No UniSampler with tube 1 300 1042 Accessories Description Pk Cat No Sample bottle 1000 ml 1 300 1018 g Spare PVC hose 2 50 m long 1 300 1043 Made of spark suppressing metals brass lead Bottle is 80 mm 350 mm high Without lowering cable e For sampling flammable liquids e Easy handling Acc DIN 51 750 Description Pk Cat No Dipping bottle Ex 1 300 1035 Accessories 7 pK Pk Cat No tai t ti 3 owering cable stainless steel conductive iam 300 1040 mm length 10 m Made of chrome plated brass or stainless steel 1 capacity 75 mm 380 mm high Lowering cable must be ordered separately e For withdrawing liquids from tanks tanker lorries etc e Easy handling Acc DIN 51 750 Description Pk Cat No Dipping vessel chrome plated brass 1 300 1033 Dipping vessel stainless steel 1 300 1034 WWR CF www vwr com Sampling Samplers Fluid Manually Operated Reel Conductive PA Specially designed for use with flammable liquids of hazard class Alll VbF Should only be used in combination with an electrically conductive lowering cable or chain type cable Please order your required lowering cable separately Reel diameter 100 mm total diameter 180 mm e High winding capacity e Width 50 mm Description Pk Cat No Manually operated reel Ex without cable 1 300 0015 Bottles Water Sampling Narrow
10. e Inert and chemically resistant to commonly used laboratory reagents at room temperature Description Pk Cat No Sterile container 110 ml with lid 100 391 0020 Sterile container 220 ml with lid 100 391 0023 g r a Erlenmeyer Flasks vVvwRe J PC clear narrow mouth y Ideal for shaker and suspension cell culture applications l e Sterile and autoclavable e Safe and shatterproof e Chemically inert Flasks are available with baffled or flat bottoms Each flask supplied with two caps one standard seal one vented Replacement caps are also available Packaging Individually packed Version Capacity ml Pk Cat No 125 24 215 2214 g Baffled 250 12 215 2216 500 12 215 2218 125 24 215 2220 Flat 250 12 215 2222 500 12 215 2224 Replacement screwcaps blue PP size 38 430 Description Pk Cat No Standard cap blue 48 iore 96 215 2207 Septum cap blue 96 215 2212 72 215 2225 Vented cap with PTFE membrane sterile 48 215 2226 The VWR e newsletter Register now at http eu vwr com e newsletter 392 35 VWR F www vwr com Erlenmeyer Flasks Corning Optically clear PC applications and storage preventing leakage e Certified non pyrogenic Baffled bottom Erlenmeyer Flasks Cell Culture Cell Culture Bottles and Tubes e Baffled or plain bottom options in all sizes 125 ml to 3 L e Moulded in graduati
11. Email uksales uk vwr com Fax 01455 55 85 86 392 60 Web http uk vwr com Cell Culture Cell Culture Media and Supplements BD Vitronectin BD Biosciences Vitronectin is also known as S protein serum spreading factor epibolin Vitronectin and fibronectin are the two major adhesive proteins in plasma and serum When used as coating on tissue culture surfaces vitronectin is useful to promote cell attachment spreading proliferation and differentiation normal and neoplastic cells and to study cell migration Lyophilised dialysed against 10 mM phosphate buffer pH 7 7 reconstitute in distilled water or buffered solution at neutral pH Source Human plasma Quality controlled by SDS PAGE and tested and found negative for bacteria fungi and mycoplasma Source material tested for hepatitis B antigen and HIV 1 antibody Formulation Quality control Molecular weight 75 kD and 65 kD components Storage and stability Stable for at least three months at 2 8 C lyophilized or one week at 2 8 C solubilised Description Pk Cat No BD Vitronectin 250 uG 734 0098 BD Cell Tak Cell and Tissue Adhesive BD Biosciences BD Cell Tak Cell and Tissue Adhesive is a formulation of polyphenolic proteins extracted from Mytilus edulis marine mussel These proteins are the key components of the glue secreted by the mussel to anchor itself to solid substrates in the marine environment B
12. _ Pk Cat No 1 Chamber 636 8 734 4059 392 51 VWR E53 www vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Accessories A variety of optional filling caps are available to allow direct aseptic transfer of media and cells via pumping or gravity feed Several coupling devices are available on these filling caps with or without integrally sealed USP Class VI certified C Flex tubing Optional filling caps with attached filters with hydrophobic membranes provide for gas exchange and faster aseptic venting during liquid transfers Extra sterile vented or unvented 33 mm replacement caps are also available Reusable stacking devices fit between CellSTACK chambers to keep them level and optimise incubator space while providing clearance for gas exchange Description Pk Cat No Universal cap with vented overcap sterile 4 734 4065 Solid cap sterile 6 734 4167 Vent cap 9 5 mm ID tubing 7 cm length Pall Acro 50 PVDF filter sterile Vent cap 9 5 mm ID tubing 7 cm Tength Pall Bacterial Air Vent sterile Vent cap 0 2 mm membrane sterile 6 734 4166 Two vented overcaps and one solid overcap for the 5 734 1108 4 734 1205 100 734 1208 universal cap sterile Fill cap 3 2 mm ID tubing female luer lock with male luer plug sterile Fill cap 6 4 mm ID tubing 70 cm length male MPC coupling with female end cap sterile Fill cap 9 5 mm ID tubing and 7 94 mm barbed fitting sterile Fi
13. 3 j www vwr com Cell Culture CO Incubators Accessories Description Pk Cat No Connection Kit CO2 390 0181 1 Connection Kit Oz 1 390 0182 Connection Kit N2 1 390 0183 Base on castors for CB 53 1 390 0321 1 1 1 Stacking adapter for CB 53 390 0322 Perforated shelf for CB 150 stainless steel 390 6062 Perforated shelf for CB 53 stainless steel 390 0316 Factory fitted options Description Pk Cat No Lockable keyboard for CB series incubators 1 390 0003 Rear 30 mm silicone access port 1 390 0317 Ethernet interface for CB series incubators 1 390 0318 CO Incubators C 150 Series Binder Temperature range ambient 7 to 50 C volume 150 The C 150 CO2 incubator meets the highest standards for cell cultivation Technically uncompromising it is ideal for routine cell culture applications The C 150 is easily accessible units can be used as stand alone or stacked The microprocessor controlled CO2 incubators have electronically controlled APT line preheating chamber technology a fan assisted air jacket system a gas mixer head and a drift free CO2 infrared absorption measuring system as well as an automatic diagnostic system with optical and acoustic alarm all as standard e Contamination free cultivation due to standard compliant hot air sterilisation at 180 C e 27 less potential contamination surface in the seamless deep drawn interior chamber e Stable pH values due to F
14. Description Total length mm Pk Cat No Cell scraper for use with 12 5 25 cm vessels 18 mm blade 180 100 734 0385 Cell scraper for use with 75 cm vessels 18 mm blade 250 100 734 0386 Cell scraper for use with 75 cm vessels 30 mm blade 250 100 734 1111 Cell scraper for use with 150 175 cm vessels 30 mm blade 400 100 734 0387 e Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 54 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Accessories and Sealing Systems Cell Scrapers Nunc Cell scrapers for cell harvesting are available in two lengths with adjustable blade for optimal application flexibility Non pyrogenic Description Total length mm Pk Cat No For use with 25 80 cm flasks blade offset 7 5 mm blade width 15 5 mm 230 250 734 2132 e For use with 75 175 cm flasks blade offset 16 mm blade width 17 5 mm 320 250 734 2133 Cell Scrapers and Cell Lifters Corning Useful for the manual harvesting of cells scrapers are designed for use in flasks and cell lifters for use in harvesting cells especially stem cells in dishes e Blade design minimises cell damage and ensures even contact with the growth surface e Individually wrapped e Sterilised by gamma radiation Certified non pyrogenic Description Pk Cat No Cell lifter 19 mm blade 180 mm handle 100 734 1526 Small cell scraper 18 mm blade 250 mm handle 100 734 1527 e
15. Sealing mats BugStopper 5 512 1044 BD Endothelial Cell Growth Supplement BD Biosciences Endothelial Cell Growth Supplement ECGS is a broadly used supplement to culture a variety of cells particularly endothelial cells ECGS contains various growth factors for example acidic FGF or ECGF a Formulation Lyophilised from NaCl containing buffer with streptomycin sulphate reconstitute in serum free medium Source Bovine brain Tested for ability to promote proliferation of Foetal Bovine Heart Endothelial Cells FBHEC in medium Quality control containing 10 newborn calf serum Filtered 0 2 um membrane and tested and found negative for bacteria fungi and mycoplasma 5 150 pg ml medium depending on cell type Some primary cells require supplementation of 1 100 ug ml heparin Stable for at least three months at 2 to 8 C lyophilised or one month at 20 C solubilised Avoid multiple freeze thaw cycles Recommended concentration Storage and stability Description Pk Cat No BD endothelial cell growth supplement 15 mg 734 1306 Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 58 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Media and Supplements BD MITO Serum Extender BD Biosciences BD MITO Serum Extender is a concentrated fully defined formulation of hormones growth factors EGF and FGF and other metabolites i
16. 0139 ENT straight wire plain medium 125 720 0140 ENT straight wire charcoal medium 125 720 0119 Pernasal twisted wire plain medium 125 720 0121 Pernasal twisted wire charcoal medium 125 720 0120 Swabs Transport Sterilin Plastic applicator blue cap rayon tip Primarily intended for sample collection and transport of bacteria these swabs contain media for the maintenance of bacteria during transport to the laboratory The pouch seal turns white on opening a visible sign of tamper evidence Each batch of product is tested for performance using a wide range of aerobic and anaerobic pathogens to ensure adequate recoveries together with sterility and other quality assurance tests e Applicator and tube will maintain the survival of a wide range of bacteria for 24 48 hours e Unique nitrogen flushed aluminium inner bag with laminated film pouch prevent oxidation and dehydration of media e Each tube is printed with lot number expiry date and has a tamper evident sleeve ensuring complete traceability and sterility e CE marked as Class lla in accordance with Medical Device Directive 93 42 EEC for transient invasive use Description Pk Cat No Transport swab Amies 500 720 0026 Transport swab Amies with charcoal 500 720 0027 Tel 0800 22 33 44 freephone Email uksales uk vwr com 300 16 Fax 01455 55 85 86 Web http uk vwr com Sampling Bags and Swabs Swabs
17. 0161 Note Growth area and capacity are nominal BD BioCoat Gelatin Cell Culture Flasks BD Biosciences PS coated with porcine gelatin sterile non pyrogenic BD BioCoat Gelatin provides an attachment and growth promoting substrate for the culture of a variety of cell types Gelatin is commonly used in the culture of normal and transfected cell types including vascular endothelial muscle embryonic stem ES and F9 teratocarcinoma cells Gelatin is a heterogeneous mixture of water soluble proteins derived through the hydrolysis of collagen Applications include promotion of cell attachment and spreading of vascular endothelial cells for instance BME BAEC ES cells C2C12 myoblasts and MM14 myoblasts culture of normal and transfected F9 teratocarcinoma cells for gene expression studies and culture of HUVEC for E Selectin expression and VEGF induction e Gelatin substrate enhances the attachment of a variety of normal and transfected cell types e Pre treatment with gelatin eliminates time consuming preparation saving time and money e Lot to lot consistency ensures reproducible results Growth area cm Capacity ml Neck Cap Pk Cat No canted vented 5 734 0162 75 250 canted vented 50 734 0293 canted plug seal 5 734 0253 canted plug seal 50 734 0324 Note culture area and capacity are nominal Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 6 Fax 01455 55 85 86 Web http uk
18. 11 5 16x100 1000 212 7522 Culture tube round bottom 2 16x129 Wo9 21251323 19 16x150 1000 212 3418 24 20x125 500 212 7525 30 20x150 500 212 7526 Culture tube flat bottom v 10x125 290 2121520 29 5 20x145 500 212 7521 Disposable Culture Tubes PS or PP Ideal for use in bacteriology radioimmunoassay RIA coagulation and other routine laboratory procedures e Manufactured without the use of release agents that could cause errors and interference in RIA tests e Precision moulding ensures uniform size and shape e PP tubes are translucent can withstand centrifugation speeds over 3000 g are suitable for most common acids solvents and alkalis at room temperature are almost unbreakable and can be sterilised at 120 C e PS tubes are transparent will withstand centrifugation speeds up to 1400 g can tolerate aqueous solutions mild bases and weak acids but not organic solvents aromatic or chlorinated hydrocarbons and cannot be autoclaved Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 42 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Bottles and Tubes Capacity Description ml xH mm Pk Cat No PS culture tubes blue 5 12x75 1000 212 9603 PS culture tubes yellow 5 12x75 1000 212 9604 PP culture tubes natural 5 12x75 1000 212 9599 PP culture tubes green 5 12x75 1000 212 9600 PP culture tubes yellow 5 12x75 1000 212 9602 PP culture tubes or
19. 125 ml flask 1 734 3152 For 250 ml flask 1 734 3153 For 500 ml flask 1 734 3154 For 1000 ml flask 1 734 3155 For 3000 ml flask 1 734 3156 For 6000 ml flask 1 734 3157 For 8000 ml flask 1 734 3158 Replacement Impeller for Celstir Flasks Wheaton This unit includes a top cap with liners glass rod magnet holder magnet and E stirring paddle for sizes 125 ml or larger f eh Description Pk Cat No Impeller for 25 ml flask 1 734 3159 Impeller for 50 ml flask 1 734 3160 Impeller for 125 ml flask 1 734 3161 Impeller for 250 ml flask 1 734 3162 Impeller for 500 ml flask 1 734 3163 Impeller for 1000 ml flask 1 734 3165 Impeller for 3000 ml flask 1 734 3166 Impeller for 6000 ml flask 1 734 3167 Impeller for 8000 ml flask 1 734 3168 392 47 VW R E F www vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Cell Production Roller Apparatus Modular with All Position Drive Wheaton This Modular Cell Production Roller Culture Apparatus allows flexibility for scale up and production of monolayer cell cultures in standard roller bottles The system consists of a base drive unit and five position roller deck to which additional roller decks can be added A powerful DC motor with soft start speed control drives the bottles through a series of durable nonslip belts The all position drive is a positive traction drive system for lightweight plastic bottles in which each roller is individually dri
20. 125 pk 1000 734 0443 PS 12x75 mm 5ml without cap sterile 125 pk 1000 734 0442 PS 12x75 mm 5ml without cap non sterile 1000 pk 1000 734 0000 PS 12x75 mm 5ml cell strainer cap sterile for flow cytometry 25 pk 500 734 0001 PS 13x100 mm 8 ml screw cap sterile 125 pk 1000 734 0439 PS 17x100 mm 14 ml snap cap sterile 1 pk 500 734 0435 PS 17x100 mm 14 ml snap cap sterile 25 pk 500 734 0444 PS 17x100 mm 14 ml snap cap sterile 125 pk 1000 525 0122 PS 17x100 mm 14 ml without cap sterile 125 pk 1000 734 0989 PS 16x125 mm 16 ml screw cap sterile 1 pk 500 734 0440 PS 16x125 mm 16 ml screw cap sterile 125 pk 1000 734 0986 PS 16x150 mm 19 ml screw cap sterile 1 pk 500 734 0441 PP 12x75 mm 5 ml snap cap sterile 1 pk 500 734 0437 PP 12x75 mm 5 ml snap cap sterile 25 pk 500 734 0447 12x 5 mm 5 ml without cap sterile p 5 PP 12x75 mm 5 ml without cap non sterile 1000 pk 1000 391 0000 PP 17x100 printed mm 14 ml snap cap sterile 1 pk 500 734 0438 1 X printed mm ml snap cap sterile 25 p l PP 17x100 printed mm 14 ml without cap sterile 125 pk 1000 734 0985 Cell Culture Tubes Nunclon A Nunc PS with PE caps sterile Round bottom tubes with screw or push on cap for standard cell culture or flat sided tubes for the culture of adherent cells e Flat side allows easy microscopy a coverslip can be used in the tube e Medium retai
21. 15 500 391 2001 t ith 1 Integrid square with 13 mm 100 15 300 391 2004 moulded in grid Made from crystal clear PS for best possible use in microscopic applications Versions with or without vents e Easily stackable e Heavy construction for good thermal resistance when working with hot agar e With vents for better gas exchange or without vents for long incubation times Packaging Packaged in sets of 20 480 or 20 600 and 15 120 plates Height Version mm _ mm Pk Cat No 35 10 740 391 2072 A 60 15 560 394 2073 With 3 vents sterile 90 16 480 391 2077 90 15 480 391 2075 With venis 94 16 480 391 2076 With vents heavy construction 100 16 420 391 2078 Without vents 94 16 480 391 3661 Without vents heavy 94 16 480 391 3662 construction With vents heavy construction 90 20 360 391 2079 With vents 145 20 120 391 3665 Square 120x120 with vents 17 240 391 2080 VWRED www vwr com Cell Culture Petri Dishes Petri Dishes Transparent PS Ideal for food industry or laboratories These dishes are produced in class 100 manufacturing environment ensuring high level of asepticity ISO 5 Product label with batch number on each carton e Excellent optical quality Stable stacking thanks to the stacking ring e High level of mechanical resistance and up to 55 C Version mm Height mm Pk Cat No With triple vents 90 14 2 825 391 1505 With on
22. 20 C in aliquots The working concentration in LB ampicillin plates LB or TB medium is 50 ug ml dilution 1 1000 Stability The activity of a 10 aqueous solution of ampicillin if stored at 4 C pH 7 is reduced by 15 after 24 hours 33 after 48 hours and 65 after 7 days At room temperature the corresponding loss of activity is 28 45 and 81 respectively Bk Cat No 10g A0839 0010 259 A0839 0025 00g COTO o ASO G418 Disulphate AppliChem G418 blocks protein synthesis in mammalian cells by interfering with ribosomal function It is an aminoglycoside antibiotic similar in structure to neomycin gentamycin and kanamycin G418 disulphate is used for the selection of stably transformed cells which have incorporated the neomycin resistance gene aminoglycoside phosphotransferase derived from the transposons Tn 5 and Tn 601 respectively Stability and Solubility G418 is stable at 4 C Solutions are stored at 20 C and are stable for up to 2 years Stock solutions of G418 should be prepared in a highly buffered solution so that addition of the drug does not alter the pH of the medium Stock solutions can be prepared at a concentration of 50 mg ml Pk Cat No 1g A2167 0001 250 mg A2167 0250 500 mg A2167 0500 Gentamycin Sulphate AppliChem Gentamycin is an aminoglycoside antibiotic and was isolated from Micromonospora species actinomycetales The sulphate salt is soluble in wate
23. 390 0375 Drosophila modification Series 3 model 300 1 390 0376 Drosophila modification Series 3 model 400 1 390 0383 Drosophila modification Series 4 model 600 1 390 0377 Drosophila modification Series 4 model 1200 1 390 0378 Inner perspex doors Description for Pk Cat No Inner perspex door Series 3 model 100 1 390 0121 Inner perspex door Series 3 model 200 1 390 0361 Inner perspex door Series 3 model 300 1 390 0362 Inner perspex door Series 4 model 600 1 390 0123 Inner perspex door Series 4 model 1200 1 390 0124 Interior illumination Description for Pk Cat No Interior illumination with 7 day timer Series 3 model 100 1 390 0364 Interior illumination with 24 hour timer Series 3 model 100 1 390 0363 Interior illumination with 7 day timer Series 3 model 200 1 390 0369 Interior illumination with 24 hour timer Series 3 model 200 1 390 0116 Interior illumination with 7 day timer Series 3 model 300 and 400 1 390 0368 Interior illumination with 24 hour timer Series 3 model 300 and 400 1 390 0366 Interior illumination with 7 day timer Series 4 model 600 1 390 0365 Interior illumination with 24 hour timer Series 4 model 600 1 390 0117 Interior illumination with 7 day timer Series 4 model 1200 1 390 0367 Interior illumination with 24 hour timer Series 4 model 1200 1 390 0118 Standard shelves Description for Pk Cat No Standard shelf Series 3 model 100 1 390 0370 Standard shelf Series 3 model 200 1 390 0371 Standard shelf Series 3
24. 4 5 9 4 16 734 2123 Chamber Slide Permanox 2 chamber 1 2 2 0 4 2 16 734 2124 e Chamber Slide Permanox 4 chamber 0 5 0 9 1 8 16 734 2125 e Chamber Slide Permanox 8 chamber 0 2 0 4 0 8 16 734 2126 e Chamber Slides Lab Tek Il Nunc Recommended working PS medium chamber on glass slide PS lid sterile For culturing cells on a standard microscope slide Useful for viral and mycoplasma testing chromosome studies toxic tests and immunocytology e Removable medium chamber of 1 2 4 and 8 well configuration attached to non fluorescent glass microscope slide 25x75x1 2 mm with rounded corners using biocompatible adhesive e Inert hydrophobic well border printed on slide e Superfrost printed writing area e Treated to ensure excellent cell attachment and growth Description volume ml Growth area cm Pk Cat No 1 Chamber configuration 0 4 5 8 6 16 734 2047 2 Chamber configuration 1 0 2 0 4 0 16 734 2048 e 4 Chamber configuration 0 5 1 0 1 7 16 734 2049 8 Chamber configuration 0 2 0 5 0 7 16 734 2050 Chamber Slides Lab Tek II CC2 Nunc each pack Recommended working e Growth surface remains stable without refrigeration e Light blue frosted writing area for clear identification e Slide separator used to lift the medium chamber from the slide included in 1 0 Borosilicate coverglass with coated growth surface PS medium chamber sterile The
25. 5 300 0502 e Small 2 26 mm ID blue handle 2 5 300 0503 Tel 0800 22 33 44 freephone Email uksales uk vwr com 391 9 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Innoculating Needles and Accessories Inoculating Loops Disposable Microloops Sterile disposable inoculating loops e Colour coded plastic e Moderately flexible Description Capacity ul Pk Cat No i 10 1000 300 0510 oop 1 1000 300 0512 10 1000 300 0500 s Lose peslipauch 1 1000 300 0511 Inoculating Loops These NiCr 8020 loops are available in a range of sizes and lengths with a internal diameters of 1 5 2 5 or 4 mm They are heat resistant and can be B flamed to sterilise the loop Diameter of wire 0 6 mm 631 7131 Description Pk Cat No Loops 50 mm 1 5 mm 10 631 7131 Loops 50 mm 2 5 mm 10 631 7132 e Loops 50 mm 4 0 mm 10 631 7133 e Loops 75 mm 1 5 mm 10 631 0084 G Loops 75 mm 2 5 mm 10 631 0085 e Loops 75 mm 4 0 mm 10 631 0086 g Holders for Needles and Loops With black plastic handle 631 0620 Description Pk Cat No Holder length 170 mm 5 631 0620 g Holder length 240 mm 5 631 0621 e ae T Inoculating Needles VWRED Plastic single use sterile Packaging Peelable sachets Description mm Length mm Colour Pk Cat No Mini inoculation needles 1 15 150 blue 500 612 2654 Inoculation needles 1 45 200 violet 1000 612 2655
26. Cat No 1 level 200 632 8 732 2603 2 Levels 400 1264 5 732 2606 10 Levels 2000 6320 2 734 2082 10 Levels 2000 6320 6 734 2105 40 Levels 8000 25280 2 734 2028 Accessories Description Pk Cat No Cover caps 400 734 2143 Start up kit sterile 1 Kit 734 2106 Connector Teflon 10 734 2110 Filter 10 391 8339 Cell Factories for Active Gassing Nunclon A Nunc PS sterile For industrial scale production of for example vaccines monoclonal antibodies or pharmaceuticals The patented gas flow system secures a controlled atmosphere in the culture trays by equal distribution of user specified gas mix actively pumped through the pre mounted filter Particularly oxygen demanding and pH sensitive cells may benefit 4 from the controlled atmosphere e Gas is equally distributed between trays as well as in each individual tray e Surface treatment ensures excellent conditions for cell attachment and growth e Available in 4 10 and 40 tray versions for easy scale up e Low contamination risk e Compatible with existing manual and automated handling equipment from Nunc Tray LxW 335x205 mm 392 53 VWR 3 j www vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Recommended working Type volume ml Growth area cm Pk Cat No 4 Levels 800 2528 10 734 1366 10 Levels 2000 6320 6 734 1339 40 Levels 8000 25280 2 734 1367 Accessories Description Pk Cat N
27. Chamber 3180 8 734 4061 10 Chamber 6360 2 734 1040 10 Chamber 6360 6 734 1041 40 Chamber 25440 2 734 4052 Corning CellBIND surface The Corning CellBIND surface enhances cell attachment under difficult conditions such as reduced serum or serum free medium resulting in higher cell yields e More consistent and better even cell attachment leads to increased cell growth and yields e Adapts cells more quickly to reduced serum or serum free conditions e Reduces premature cell detachment from confluent cultures e May eliminate the need for tedious time consuming low stability biological coatings e Requires no refrigeration or special handling and is stable at room temperature Type Growth area cm _ Pk Cat No 1 Chamber 636 8 734 1017 2 Chamber 1272 5 734 1014 5 Chamber 3180 2 734 4060 10 Chamber 6360 2 734 1207 10 Chamber 6360 6 734 1015 40 Chamber 25440 2 734 1016 Ultra Low Attachment surface The Ultra Low Attachment surface is a covalently bonded hydrogel surface that is hydrophilic and neutrally charged It minimises cell attachment protein absorptiom and enzyme activation The surface is non cytotoxic biologically inert and non degradable e Maintains cells in a suspended unattached state e Prevents stem cells from attachment mediated differentiation e Prevents anchorage dependent cells from dividing e Reduces binding of attachment and serum proteins to the substrate Type Growth area cm
28. Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Plates and Dishes BD BioCoat Poly Lysine Cell Culture Dishes BD Biosciences PS coated with poly lysine Poly D Lysine PDL and Poly L Lysine PLL are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate Poly Lysine surface treatments support applications including attachment and spreading of a variety of cell lines cell differentiation and neurite outgrowth attachment of transfected cell lines and survival of primary neurons in culture BD BioCoat Poly D Lysine Description Pk Cat No Culture dishes 35 mm 100 734 0417 Culture dishes 60 mm 100 734 0284 Culture dishes 100 mm 40 734 0285 BD BioCoat Poly L Lysine Description Pk Cat No Culture dishes 35 mm 100 734 0302 Culture dishes 60 mm 100 734 0301 BD Falcon In Vitro Fertilisation Plasticware BD Biosciences Crystal grade PS sterile with lid Pre tested and certified plasticware for in vitro fertilisation IVF designed for consistency and ease of use These plates have perfectly flat optically clear surfaces for optimum manipulation and observation of ova and embryos Lids are designed for aseptic manipulation and consistent venting to maintain humidification e Non embryotoxic non pyrogenic and non cytotoxic e Tissue Culture treated for a consistent hydrophilic surface
29. LABOCAP red 17 18 10 391 5932 Erlenmeyer Caps 5 Aluminium e Anodised and blue coloured Springs made of chromium nickel steel e Springs clamp against the outer wall of the vessel e Autoclavable Description for mm Pk Cat No Caps aluminium 37 39 10 391 0951 g Tel 0800 22 33 44 freephone Email uksales uk vwr com 391 13 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Flasks BD Falcon Cell Culture Flasks BD Biosciences PS tissue culture treated sterile non pyrogenic seme Vacuum gas plasma tissue culture treatment ensures consistent cell attachment spreading and growth e Easy to read printed volumetric graduations and writing patch e Vented caps incorporate a 0 2 um hydrophobic membrane e Phenolic caps contain non toxic liners e Convenient peel open medical style packaging ensures flask sterility is maintained Growth area cm Capacity ml Neck Cap Pk Cat No 125 25 canted plug seal 100 734 0010 25 canted vented 100 734 0043 0 canted plugseal 200 734 0009 gt 50 canted vented 100 734 0044 e 25 70 canted plug seal 200 734 0031 70 canted vented 100 734 0045 70 canted phenolic 200 734 0030 250 straight plug seal 100 734 0012 G 250 straight vented 100 734 0046 75 250 straight phenolic 100 734 0011 250 canted plug seal 60 734 0049 250 canted vented 60 7
30. Mouth with Screw Cap HDPE translucent with red screw cap Space saving square bottles e Capped ionised 10 KGray e Excellent shock resistant e Excellent tightness Capacity Neck I WxDxH ml mm mm Colour with Pk Cat No Assembled tamper evident screw cap 1000 28 82x82x182 natural f 84 215 2288 with shaped seal Twist Seal Bags VWRES LDPE bags manufactured and packaged in sterile conditions yy Universal use for samples such as foods liquids grains petroleum products animal material waste and soil samples e Wire tab keeps bag open for filling e Unique fold over system providing a temporary leak proof seal Double regular wire without write on panel Capacity ml Dimensions mm Pk Cat No 60 16x127 500 129 9831 120 16x178 500 129 9832 450 114x190 500 129 9833 540 114x229 500 129 9834 e 120 140x229 500 129 9837 810 114x305 500 129 9835 g 1080 114x382 500 129 9836 e 1260 140x382 500 129 9838 g 1650 178x305 250 129 9839 e Double regular wire with write on panel Capacity ml Dimensions mm Pk Cat No 60 16x127 500 129 9842 2 7 0 T2 ss 540 114x229 500 129 9844 e 120 140x229 500 129 9846 a i i i isSCsS C 1650 178x305 250 129 9847 Tel 0800 22 33 44 freephone Email uksales uk vwr com 300 8 Fax 01455 55 85 86 Web http uk vwr com Sampling Bags and Swabs Flat regular wire without write on panel
31. Plates 96 Well Nunclon A Nunc PS plate with either polymer or coverglass base sterile with lid sem These plates with white or black upper structure and clear base are designed to provide optimum clarity for viewing well contents e Flat bottom well geometry for plate reader access e Footprint compatible with standard equipment and automated systems e Surface treatment ensures optimal cell attachment and growth e No 1 5 coverglass base for minimum light scatter and low autofluorescence ensures accurate results due to higher signal to noise ratios Working range 50 200 ul well Description Colour Pk Cat No 96 Well optical bottom plate with polymer base black 30 AAIE g black 30 734 2088 96 Well optical bott late with lass b 6 Well optical bottom plate with coverglass base white 30 734 2089 392 15 VW R E F www vwr com Cell Culture Cell Culture Plates and Dishes Cell Culture Plates 384 Well Nunclon A Nunc PS tissue culture treated sterile Ideal for cell culture cell fusion cloning and viral titrations e Nunclon A surface treatment for optimal cell growth and attachment e Non toxic non pyrogenic e Rounded square wells eliminate wicking e Optimised for scintillation counting Working range 10 100 ul Description Colour Pk Cat No 384 Well plate flat bottom with lid transparent 30 734 2091 384 Well plate flat bottom without lid transparent 10
32. Primaria products is homogeneous and stable and is used to improve attachment and differentiation of a variety of cell types The surface chemistry of BD Primaria products is confirmed by Electron Scanning for Chemical Analysis ESCA e Unique nitrogen containing tissue culture surface chemistry improves attachment spreading and growth for many primary cells or cell lines e Optically clear and no special storage required e Convenient peel open medical style packaging Description Volume ml Growth area cm Pk Cat No BD Primaria 6 well cell culture plate 15 5 9 6 50 734 0077 BD Primaria 24 well cell culture plate 3 5 2 0 50 734 0078 BD Primaria 96 well cell culture plate 0 37 0 32 50 734 0079 Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 16 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Plates and Dishes BD BioCoat Collagen I Cell Culture Plates BD Biosciences PS coated with rat tail collagen type I sterile non pyrogenic seme Collagen is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture In vitro use of collagen can exert effects on the adhesion morphology growth migration and differentiation of a variety of cell types Applications include promotion of cell attachment and spreading rapid expansion of cell populations serum free or reduced ser
33. T0 light blue 40 7000 612 9362 Loops 1 45 mm long 20 cm violet 40 1000 612 9366 e Accessories Description Capacity ul Colour Packed Pk Cat No Holder for resealable packs transparent 1 612 9365 F Inoculating Loops and Needles in Dispenser Tubes VWRES Calibrated disposable inoculating loops made from flexible or rigid plastic to cater to different applications and preferences of microbiologists e Colour coded for easy product identification e Conveniently packaged in anti roll tubes with push on resealable cap e Fast easy and secure access to the loops without the need for a separate holder e Loops have an ultra smooth surface for easy streaking and length of 210 mm Delivery Information Calibration certificate supplied with every pack Description Capacity ul Colour Packed Pk Cat No Inoculating loops rigid 1 dark green 50 1000 612 2494 Inoculating loops flexible 1 light green 50 1000 612 2497 Inoculating loops rigid 10 dark blue 50 1000 612 2496 Inoculating loops flexible 10 light blue 50 1000 612 2498 Inoculating needles 1 45 mm violet 50 1000 612 2495 g Dehydrated or ready to use media All you need See Microbiology section Chapter 7 391 8 VW R E F www vwr com Inoculating Loops Cell Culture Innoculating Needles and Accessories VWRES These loops ensure consistent wetting and complete liquid transfer Available r
34. Top Roller Culture System Wheaton This conventional type roller culture equipment is designed to roll vessels 108 to 121 mm in diameter and up to 290 mm long e Compact unit well suited for research work e Two outside rollers can be moved inward to accommodate bottles as small as 75 mm in diameter e Each deck can accommodate two vessels vessels not supplied Power 230 V 50 60 Hz 35 W Bottle speed 0 1 to 3 8 rom based on 110 mm bottle WxDxH 320x325x180 mm Weight 8 kg Warning Use in an environment rich in carbon dioxide might necessitate more frequent replacement of motor brushes Not to be used in temperatures exceeding 40 C Description Pk Cat No Single deck roller apparatus UK plug 1 734 1168 Deck kit for small bottle roller culture system Attaches quickly and easily to the Small Bottle Bench Top Roller Culture System Holds an additional 2 rollers per kit These units will accept two additional decks Kit consists of assembled roller deck four support posts drive belt necessary hardware and assembly instructions Weight 2 3 kg Description Pk Cat No Deck kit for small bottle roller culture system 1 734 1170 Mini Bottle Bench Top Roller Culture System Wheaton The Mini Bottle Bench Top Roller Culture System is designed for small scale mixing and agitation using bottles that are too small to be accommodated by standard roller apparatus It is ideal for 30 ml or larger serum bottles 100 125
35. WxL 25x75 mm with 1 2 mm bevelled edge Recommended working volume Growth area Description ml cm Pk Cat No BD BioCoat Collagen CultureSlides 1 well 5 0 6 5 8 6 12 734 0205 BD BioCoat Collagen CultureSlides 2 well 2 0 2 5 4 0 12 734 0238 BD BioCoat Collagen CultureSlides 4 well 1 0 1 5 1 7 12 734 0206 BD BioCoat Collagen CultureSlides 8 well 0 8 0 8 0 7 12 734 0241 per well Chamber Slides Lab Tek Nunc PS medium chamber on glass or Permanox plastic slide PS cover sterile Useful for viral and mycoplasma testing chromosome studies toxicity tests and immunocytology Permanox is a TC treated plastic surface with minimal fluorescence Cells grow on a standard microscope slide e No cell transfer needed prior to visualisation staining e Upper chamber can be removed when culturing is complete e Suitable for use with fluorescent labels 392 25 VWR F www vwr com Cell Culture Cell Culture Chamber Slides Recommended Description working volume ml Growth area cm Pk Cat No Chamber Slide glass 1 chamber 9 4 16 734 2119 Chamber Slide glass 2 chamber 1 2 2 0 4 2 16 734 2120 Chamber Slide glass 4 chamber 0 5 0 9 1 8 16 734 2121 e Chamber Slide glass 8 chamber 0 2 0 4 0 8 16 734 2122 Chamber Slide glass 16 chamber 0 1 0 2 0 4 16 734 2127 e Chamber Slide Permanox 1 chamber 2 5
36. and PLL are synthetic molecules they do not stimulate biological activity in the cells cultured on them and they do not introduce impurities carried by natural polymers BD BioCoat Poly D Lysine Description Colour Pk Cat No 6 Well plate transparent 5 734 0120 12 Well plate transparent 5 734 0151 24 Well plate transparent 5 734 0121 48 Well plate transparent 5 734 0174 392 17 vwRe F www vwr com Cell Culture Cell Culture Plates and Dishes Description Colour Pk Cat No transparent 5 734 0146 black clear 5 734 0245 black clear 50 734 0317 e 96 Well plate white clear 5 734 0250 white clear 50 734 0321 white 5 734 0237 white 50 734 0316 black clear 50 734 0328 white clear 50 734 0325 384 Well plate transparent 50 734 0327 white 50 734 0326 BD BioCoat Poly L Lysine Description Colour Pk Cat No 6 Well plate transparent 50 734 0299 96 Well plate transparent 50 734 0300 BD BioCoat Fibronectin Cell Culture Plates BD Biosciences PS coated with fibronectin sterile non pyrogenic Fibronectin coated cell culture plates have a number of applications including the promotion of cell attachment proliferation and differentiation of a variety of cell types particularly fibroblasts and other mesenchymally derived cells Description Colour Pk Cat No 6 Well plate transparent 5 734 0110 24 Well plate transparent 5 734 1316 96 Well plat
37. angiogenesis studies and augmentation of tumour growth in nude mice e Typical protein concentration 18 22 mg ml e Lot specific specification sheet supplied with each delivery e Tested for the ability to promote neurite outgrowth of chick dorsal root ganglia cells and for the ability to gel quickly and maintain its form with culture medium for a period of 14 days at 37 C e Bacteria fungi and mycoplasma free e Endotoxin tested by LAL assay Description Pk Cat No BD Matrigel Matrix high concentration phenol red free 10 ml 734 1402 BD Matrigel Matrix high concentration 10 ml 734 0273 392 61 VWR 3 j www vwr com Cell Culture Cell Culture Media and Supplements BD Matrigel Basement Membrane Matrix BD Biosciences BD Matrigel Basement Membrane Matrix is a solubilised basement membrane preparation extracted from the Engelbreth Holm Swarm EHS mouse sarcoma a tumour rich in extracellular matrix proteins Its major components are laminin followed by collagen IV entactin and heparan sulphate proteoglycan It also contains growth factors that occur naturally in the EHS tumour At room temperature BD Matrigel Matrix polymerizes to produce biologically active matrix material resembling the mammalian cellular basement matrix BD Matrigel Basement Membrane Matrix is effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelial and other cell types The Growth F
38. cap 70 mm for 3L flask 24 734 1216 Flat cap 48 mm for 2L flask 24 734 4194 Flat cap 70 mm for 3L flask 24 734 4195 Media Bottles with Cap VWRED PC autoclavable narrow mouth bottles with standard PP caps y VWR Collection Media Bottles are an ideal replacement for all borosilicate glass bottles They are specifically designed for every laboratory application requiring terminal sterilisation autoclaving storage transportation and production of laboratory biological fluids The bottles are available in square and round shapes to accommodate various packaging needs The closure system is guaranteed leak proof and the seal ring allows for convenient shrink wrapping of the caps Each bottle is carefully produced and quality controlled for uniform wall thickness clarity graduation and leak proof closure Optional septum cap or vented cap with PTFE membrane is also available e Ideal for terminal sterilisation e Safe and shatterproof e Chemically inert Description Thread Pk Cat No Clear square 1000 ml 38 430 12 215 2206 Clear square 500 ml 38 430 12 215 2205 Clear square 125 m 38 430 24 215 2203 Clear round 1000 ml 38 430 12 215 2211 g Clear round 500 ml 38 430 12 215 2210 g Clear round 125 m 38 430 24 215 2204 g Amber square 1000 ml 38 430 12 215 2202 Amber square 500 ml 38 430 12 215 2201 Amber square 125 m 38 430 24 215 2200 g Amber roun
39. clean as all surfaces are free of pores and crevices preventing accumulation of dirt Colour coded push buttons in red blue green yellow white and black 250 ml containers e Disassembly and cleaning is extremely simple e Easy to use one hand push button operation e Transparent Suitable for point sampling cross sectional sampling bottom sampling According to DIN 53 242 Description Pk Cat No LiquiSampler PTFE FEP 1 m 1 331 2150 LiquiSampler PP 1 m 1 331 2152 x Accessories Description Pk Cat No Tube cleaning brush 25 mm 1 m 1 331 2170 The single use flexible suction hose can be quickly replaced thus ensuring that the samples are never contaminated If necessary a new hose can be used for every single sample Due to its small diameter 8 mm and flexibility the hose can reach sampling areas which are otherwise inaccessible e Can be used with oils e Various accessory bottle sizes available Delivery Information Supplied complete with 10 bottles PE 100 ml in a stable case Description Pk Cat No MiniSampler PE complete 1 300 1021 Accessories Description Pk Cat No Suction hose PE 6 mm wall thickness 1 mm 100 m 300 1045 e Made of transparent PP or ultra pure and chemically inert PTFE FEP for contamination free sampling The media is drawn up and discharged using the suction flask Specially developed for viscous substances for example slurries silt wet cla
40. headspace for additives Delivery Information Supplied with caps pre attached sterile and shrink wrapped Capacity ml Dimensions mm Pk Cat No 125 53x53x99 48 215 8320 500 15x75x175 24 215 8321 e Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 38 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Bottles and Tubes Storage Bottles Corning Easy Grip Style Corning PS Disposable polystyrene bottles for storage of media buffers and other seme aqueous solutions e Low profile easy grip style has sides that facilitate handling e Plug seal caps 45 mm provide an airtight seal and help minimise the risk of contamination Bottles can be used with Corning Vacuum Filter Systems e Sterile certified non pyrogenic Description Capacity ml Cap size mm Pk Cat No Corning Easy Grip storage bottles 150 45 24 734 1897 Corning Easy Grip storage bottles 250 45 24 734 1824 Corning Easy Grip storage bottles 500 45 24 734 1825 Corning Easy Grip storage bottles 1000 45 24 734 1847 Caps for Test Tubes Safe T Flex VWRED Safe T Flex caps feature a unique flexible over locking design locking securely on the outside of the tube lip and removable by one hand A tight yy seal ensures sample integrity and prevents spillage or evaporation of serum _ samples This special seal protects against the aerosols of highly infectious organisms such as TB and th
41. interior fixtures easy to clean due to shape and material Control Multifuctional microprocessor PERFECT P D controller with 8 digit alphanumeric digital display digital 7 day programme timer with real time clock precise minute setting for one set value or start of ramp operation Safety Independent digital adjustable electronic microprocessor overtemperature controller TWW protection class 3 3 Connections USB interface Technical data Temperature deviation lt 0 1 C in real time Calibration Calibration and validation to assist with GLP compliance are possible please enquire Accessories Wide range of options including day night simulation 24 hour cycle programmable in conjunction with interior lighting or programming and documentation using interfaces Celsius software integrated log memory and chip cards Please ask for details Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No ICP 400 53 400x330x400 558x486x967 1 466 5209 ICP 500 108 718x556x1047 560x400x480 1 466 5056 ICP 600 256 958x656x1335 800x500x640 1 466 5210 ICP 700 416 1198x656x1495 1040x500x800 1 466 5057 ICP 800 749 1198x756x1895 1040x600x1200 1 466 5058 Tel 0800 22 33 44 freephone Email uksales uk vwr com 390 9 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Incubators Cooled Incubators with Peltier Forced Convection IPP Series Memmert Temperature range 5 to 70 C volumes 32 to 108 These
42. ml media bottles or 38x200 mm culture tubes for the growth and observation of single chicken or rat embryos The compact size makes it suitable for laboratories with limited space and for use with standard incubators and cold rooms Each single deck unit will accommodate a minimum of four bottles e Continuous rotation of cylindrical bottles at predetermined optimum speeds provides better distribution of media and uniform gassing e System allows precise speed control of the bottles and is designed to compensate for sudden or prolonged line voltage changes e Roller shafts are mounted in self lubricating nylon bearings which eliminates many maintenance problems e Able to accommodate bottles 38 60 mm in diameter up to 240 mm long with bottle speeds of 3 45 rpm 38 mm bottle and 2 30 rpm 60 mm bottle e Supplied without vessels available separately Power 230 V 50 60 Hz 14 W WxDxH 320x325x180 mm Weight 8 kg Warning Use in an environment rich in carbon dioxide might necessitate more frequent replacement of motor brushes Not to be used in temperatures exceeding 40 C Description Pk Cat No Single deck roller apparatus unit UK plug 1 734 1165 392 49 VWR E53 www vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Deck kit for mini bottle roller culture system Mini Bottle Deck Kit can be added to Mini Bottle Roller Culture Apparatus to accommodate an additional four bottles per deck It is possible
43. mm WxDxH int mm max Pk Cat No BASIC INB 200 32 550x400x600 400x250x320 1 3 1 390 0269 BASIC INB 300 39 630x400x600 480x250x320 1 3 1 390 0297 BASIC INB 400 53 550x480x680 400x330x400 2 4 1 390 0298 BASIC INB 500 108 710x550x760 560x400x480 2 5 1 390 0299 EXCELLENT INE 200 32 550x400x600 400x250x320 1 3 1 390 0300 EXCELLENT INE 300 39 630x400x600 480x250x320 1 3 1 466 5060 EXCELLENT INE 400 53 550x480x680 400x330x400 2 4 1 390 0301 EXCELLENT INE 500 108 710x550x760 560x400x480 2 5 1 390 0302 EXCELLENT INE 550 153 630x650x920 480x500x640 2 7 1 390 0324 EXCELLENT INE 600 256 950x650x920 800x500x640 2 7 1 390 0303 EXCELLENT INE 700 416 1190x650x1080 1040x500x800 2 9 1 466 5061 EXCELLENT INE 800 749 1190x750x1620 1040x600x1200 2 14 1 466 5062 390 4 VWR F www vwr com Cell Culture Incubators Shelves Inner supplied Type volume I WxDxH ext mm WxDxH int mm max Pk Cat No PERFECT INP 200 32 550x400x600 400x250x320 1 3 1 390 0304 PERFECT INP 300 39 630x400x600 480x250x320 1 3 1 390 0305 PERFECT INP 400 53 550x480x680 400x330x400 2 4 1 390 0306 PERFECT INP 500 108 710x550x760 560x400x480 2 5 1 390 0307 PERFECT INP 550 153 630x650x920 480x500x640 2 7 1 390 0325 PERFECT INP 600 256 950x650x920 800x500x640 2 7 1 390 0308 PERFECT INP 700 416 1190x650x1080 1040x500x800 2 9 1 390 0309 PERFECT INP 800 749 1190x750x1620 1040x600x1200 2 14 1 390 0310 Microbiological Incubators Heraeus Function Line Thermo S
44. model 300 1 390 0372 Standard shelf Series 3 model 400 1 390 0373 Standard shelf Series 4 all models 1 390 0128 Climatic Chambers KBF KBF ICH and KBF LQC series 4 a _ 390 0230 Temperature range 0 to 70 C without humidity volumes 115 to 700 390 0218 390 0326 The KBF series is ideal for reliable stability tests and precise maintenance of constant climatic conditions Constant temperature and humidity values are the outstanding features of this range These constant climate chambers have large reserve capacity and a variety of optional features They also comply with all applicable guidelines with respect to programming and documentation requirements such as ICH FDA GMP and GLP KBF series for precise simulation of constant climatic conditions Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com Web http uk vwr com 390 15 Cell Culture Incubators KBF ICH series a KBF with ICH compliant illumination the one source solution for photostability tests KBF LQC series a KBF with ICH compliant illumination and patented light measurement Light Quantum Control LQC e Can be operated with tap water with a maximum hardness of 8 0 dH 1 4285 mmol l Binder Pure Aqua Service flexible water purification system or deionised water e Electronically controlled humidification and dehumidification system with capacitive humidity sensor e Environmental friendly re
45. of mycoplasma in cell culture The PCR Mycoplasma Test Kit is designed to detect the presence of mycoplasma contaminating biological materials such as cultured cells Mycoplasma detection by the direct culture procedure is time consuming and some mycoplasma species are difficult to cultivate With PCR testing results are obtained within a few hours since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis There is no need to prepare probes labelled with radioisotopes or to calculate enzyme dNTP s or buffer concentrations Instead a ready to use optimised PCR mix is supplied The primer set allows detection of various mycoplasma species M fermentans M hyorhinis M arginini M orale M salivarium M hominis M pulmonis M arthridtidis M bovis M pneumoniae M pirum M capricolum as well as Acholeplasma and Spiroplasma species with high sensitivity and specificity Description Pk Cat No PCR Mycoplasma Test Kit 10 tests 1 Kit _A3744 0010 e PCR Mycoplasma Test Kit 20 tests 1 Kit A3744 0020 Myco 3 AppliChem For the treatment of Mycoplasma infected cells Myco 3 is a 100x concentrated solution based on the ciprofloxacin antibiotic which is a member of the fluoroquinolone group Many mycoplasma species have been found to be sensitive to Myco 3 including A laidlawii M orale M hyorhinis M fermentans and M arginini These spec
46. red 1 Roll 312 1151 e Printed green 1 Roll 312 1152 e Printed yellow 1 Roll 312 1153 Printed black 1 Roll 312 1154 g Unprinted white 1 Roll 312 1155 g Accessories Description Pk Cat No Dispenser 95x95 mm 1 312 1156 Sterile Sample Bottles for Water Testing VWR CF HDPE The flasks are dosed with sodium thiosulphate which inhibits the effects of wW chlorine bromine and ozone present in the water when the sample is taken Square bottles for more efficient storage apart from 250 ml bottles rectangular Shelf life 2 years kes Ii LEER e Dosage in compliance with standards 20 mg l French standard NFT90 Fre Ti es 400 100 mg l ISO 5667 3 n oer e Sodium thiosulphate in powder form v m Tae e Tamper evident closure which guarantees the sterility of the bottles prior to use Capacity ml with Cap Packed Pk Cat No 250 5 mg NazS2O3 Sealed screw cap Bulk 312 331 3407 e 5 mg Na2S20 Sealed screw cap Individually 200 331 3408 500 mg Na29203 ealed screw cap u s a 10 mg NazS2Os Sealed screw cap Individually 100 331 3412 1000 20 mg NazS2Os Sealed screw cap Bulk 84 331 3417 20 mg NazS2O3 Sealed screw cap Individually 84 331 3402 500 10 mg NazS2Os Hinged cap Bulk 100 331 3403 10 mg NazS2O0s Hinged cap Individually 100 331 3405 a a Sample Dippers VWRED PTFE inert w Extendible screw in shafts have a steel core for rigidity and are fully encapsulated in PTFE
47. system condensation free double pan humidification system e Contamination free cultivation due to overnight hot air sterilisation at 180 C Complies with DIN 58947 e Stable pH values due to short recovery times with drift free FPI infrared CO2 measurement system Design Tightly sealing inner glass door lockable outer door prevents unauthorised access or interference Weldless deep drawn inner chamber made from stainless steel with integral shelf support system Interior is easy to clean as it has no fan or HEPA filters Units can be stacked on top of eachother with optional stacking adapter to save space Shelves 3 perforated stainless steel shelves Cleaning and disinfection Hot air sterilisation at 180 C Safety Gas mixing head A special mixing head mixes the gas which is blown at 1 bar overpressure from the CO2 supply via a solenoid valve into the inner chamber with filtered air thus ensuring homogenous distribution Electronic self diagnostic system for errors with optical and acoustic alarm as well as relay contact for central monitoring Control Colour screen controller for temperature and COz concentration user friendly LCD screen and easy to read menu guide integrated electronic chart recorder real time clock and a variety of options for graphic display of process parameters APT line air jacket system Gas mixing head infrared irradiation CO2 measurement system digital drift free infrared CO2 measurement system w
48. to add up to a maximum of two kits per unit Weight 4 1 kg Description Pk Cat No Deck kit for mini bottle roller culture system 1 734 1163 BD Falcon Roller Bottles BD Biosciences PS tissue culture treated with PE caps sterile non pyrogenic Roller bottles are used in both research and manufacturing applications involving the scale up of mammalian cells for purposes of virus propagation and bioproduct production e One piece design compatible with manual or automated filling systems e Bottle manufacturing process provides greater impact resistance reducing the risk of accidental loss e Available with smooth or pleated surface and standard cap or vented cap with hydrophobic 0 2 um microporous membrane Manufactured in a Class 100 000 cleanroom Growth area Description Volume ml cm Packed Pk Cat No 2 bag 20 734 0455 th surf tandard ff Smooth surface standard easy on off cap aoe 850 201bag 0 734 0008 Smooth surface vented easy on off cap 1 bag 20 734 1274 Pleated surface standard easy on off cap 1450 20 bag 20 734 0029 Roller Bottles for Cell Culture DURAN Group DURAN glass bottle with DIN thread GL45 Supplied with blue PP screw cap and pouring ring ext xH mm Pk Cat No 110x285 1 391 0670 110x450 1 391 0672 Tel 0800 22 33 44 freephone Fax 01455 55 85 86 e business more than just a webshop For all of your laboratory needs Please v
49. vent 3 0 8 8 500 734 2041 35x10 mm without grid with air vent 3 0 8 8 500 734 2045 g 35x10 mm with 2x2 mm grid and air vent 3 0 8 8 500 734 2114 35x10 mm untreated without grid with air vent 3 0 8 8 500 734 2109 60x15 mm without grid with air vent 5 0 20 8 500 734 2113 60x15 mm without grid or air vent 5 0 21 5 400 734 2042 60x15 mm without grid with air vent 5 0 21 5 400 734 2040 60x15 mm with 2x2 mm grid and air vent 5 0 21 5 400 734 2103 100x15 mm without grid with air vent 125 567 480 734 2112 150x20 mm without grid with air vent 35 145 80 734 1403 x249x25 mm without grid or air vent e BD Primaria Cell Culture Dishes BD Biosciences PS sterile with lid The gases used to manufacture BD Primaria contain both oxygen and ammonia resulting in the incorporation in the surface of a variety of nitrogen containing functional groups in addition to the negatively charged oxygen containing groups found on traditional tissue culture treated surfaces The incorporation of nitrogen containing cations has been correlated to attachment and spreading of primary endothelial cells in a clonal cell growth assay The complex surface on BD Primaria products is homogeneous and stable and is used to improve attachment and differentiation of a variety of cell types The surface chemistry of BD Primaria products is confirmed by Electron Scanning for Chemical Analysis ESCA e Unique nitrogen containing tissue
50. vwr com Cell Culture Cell Culture Flasks BD BioCoat Poly D Lysine Cell Culture Flasks BD Biosciences PS coated with Poly D Lysine sterile non pyrogenic Poly D Lysine PDL is a synthetic compound that enhances cell adhesion and protein absorption by altering surface charges on the culture substrate Poly Lysine surface treatments support applications including attachment and spreading of a variety of cell lines cell differentiation and neurite outgrowth attachment of transfected cell lines and survival of primary neurons in culture As PDL is a synthetic molecule it does not stimulate biological activity in the cells cultured on it and it does not introduce impurities carried by natural polymers Growth area cm Capacity ml Neck Cap Pk Cat No 25 70 canted vented 50 734 0311 canted plug seal 50 734 0288 75 250 canted vented 50 734 0418 canted plug seal 50 734 0306 150 600 straight vented 40 734 0312 straight plug seal 40 734 0294 175 750 straight vented 40 734 0419 straight plug seal 40 734 0308 Note culture area and capacity are nominal Cell Culture System OptiCell Nunc UU wil TTT LEE LL OptiCell kits and chambers OptiCell is a cell culture system for growing monitoring and transporting cells It consists of two parallel gas permeable cell culture treated PS membranes attached to a standard microtitre plate sized frame Each side has a grow
51. with manually adjustable stop valve Design Inner glass doors chamber made from stainless steel Shelving 2 chrome shelves Safety Protection against overheating with a visual alarm safety thermostat K 3 1 Technical data Temperature accuracy 0 2 C Weight Incu line 53 45 kg Incu line 115 68 kg Connection Voltage 50 60 Hz 230 V Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No Incu Line 53 UK plug 53 620x622x680 401x330x401 1 390 0351 Incu Line 115 UK plug 115 820x732x760 600x480x400 1 390 0354 Accessories Description for Pk pat Ne DL 53 VL 53 IL 53 bs Careme pinted shelt DL T15 VL 11571 115 1 466 3523 Small Incubators B28 Binder Temperature range 30 to 70 C volume 28 I A compact device with hydraulic mechanical control and adjustable ventilation for precise reliable incubation conditions Optimum temperature range at 37 C e Robust and space saving e Inner glass door e Available with or without independent temperature safety regulator TC class 1 Design The housing is made from galvanised sheet steel and powder coated throughout to afford maximum protection against corrosion Control Hydraulic mechanical thermostat with analogue dial thermometer Connection 230 V 50 60 Hz 253 W Technical data Weight 22 kg Shelves Inner volume WxDxH ext WxDxH int supplied eas without temperature 0 mm mm mex k gnia 28 580x425x402 400x250x280 2 4 1 390
52. 0 710 3508 i 90 15 600 710 3507 g Stackable without vents 90 13 600 710 3514 With 4 vents 90 15 600 710 3515 Whenua 90 20 384 710 3510 sated 90 15 600 710 3516 With 2 compartments 90 15 600 710 3509 g With 3 compartments 90 15 600 710 3518 Large diameter 100 15 264 710 3503 Tel 0800 22 33 44 freephone Email uksales uk vwr com 391 1 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Petri Dishes Height Version mm mm Pk Cat No Large Diameter 140 15 120 710 3521 e Without vents 140 15 120 710 0599 With 6 vents 140 20 90 710 0600 Without vents 140 20 90 710 0601 With 6 vents 140 25 72 710 0602 Without vents 140 25 72 710 0603 Contact plate 60 25 600 710 0598 BD Falcon Petri Dishes Sterile BD Biosciences Petri Dishes Greiner bio one 391 2 PS Manufactured in accordance with the current FDA Quality System e Products are gamma irradiated e Flat distortion free optics e Stacking rings allows for easier stacking and handling e Durable construction for stable dish manipulation Height Version mm mm Pk Cat No BD Optilux 100 20 200 391 1996 g 2 compartments 100 15 500 391 1995 4 compartments 100 15 500 391 1999 Tight fit lid 50 9 500 391 1997 Integri d with 20 ntegrid roun i wi ai 150 25 100 391 2000 moulded in grid 100 15 500 391 2002 g Standard 150 15 100 394 2003 Easy Grip 35 10 500 391 1998 Easy Grip 60
53. 0 734 2083 384 Well plate flat bottom with lid black 30 734 2084 384 Well plate flat bottom without lid black 100 734 2085 384 Well plate flat bottom with lid white 30 734 2090 384 Well plate flat bottom without lid white 100 734 2095 Lids for Microtitre Plates Nunc Description Pk Cat No Lid for 96 well MicroWell plate with cut off corners and condensation rings 100 734 2184 Lid for 96 well MicroWell plate sterile with cut off corners and condensation rings 50 734 2185 Lid for 384 well MicroWell plate with cut off corners and evaporation barrier individually wrapped 60 732 2732 Lid for 384 well MicroWell plate sterile with cut off corners and evaporation barrier 180 732 2733 Low profile lid for standard height 384 well plates and OmniTrays 120 732 2752 Universal lid non sterile for standard height 384 and 1536 well plates 180 732 2704 BD Primaria Multiwell Cell Culture Plates BD Biosciences PS sterile with lid The gases used to manufacture BD Primaria contain both oxygen and ammonia resulting in the incorporation in the surface of a variety of nitrogen containing functional groups in addition to the negatively charged oxygen containing groups found on traditional tissue culture treated surfaces The incorporation of nitrogen containing cations has been correlated to attachment and spreading of primary endothelial cells in a clonal cell growth assay The complex surface on BD
54. 065 angled filter 100 734 2066 175 55 angled vent close 30 734 2168 angled filter 30 734 2167 Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com Web http uk vwr com 392 2 Cell Culture Cell Culture Flasks Caps for EasyFlasks HDPE sterile Description Pk Cat No Vent close caps for 25 cm flasks 100 734 2166 Filter caps for 25 cm flasks 100 734 2165 Vent close caps for 75 cm flasks 100 734 2164 Filter caps for 75 cm flasks 100 734 2163 Vent close caps for 175 cm flasks 100 734 2144 Filter caps caps for 175 cm flasks 100 734 2145 Cell Culture Flasks Non Treated Nunc For culturing cells that do not require a treated surface Ideal for hybridoma PS flask HDPE filter cap sterile non pyrogenic sem and suspension cultures e Canted neck flask for better accessibility e Distinctive white cap for easy identification e Extra caps provided in each carton Capacity ml Recommended working volume ml Pk Cat No 70 T 200 734 2104 e 260 30 100 734 2069 645 55 30 734 1190 VWR Collection Please contact your local VWR sales office or go to wwwawrcam for the latest news and special offers 392 3 VWR 5 j www vwr com Cell Culture Flasks Corning 734 1532 Optically clear PS 734 1713 Cell Culture Cell Culture Flasks 734 0093 Available with a choice of treated surface Co
55. 0x800 1 390 6034 BD 720 E2 720 1234x865x1528 1000x600x1200 1 390 6035 Models with forced convection Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No BF 53 53 634x575x617 400x330x400 1 390 0006 BF 115 115 834x645x702 600x400x480 1 390 0007 BF 240 240 1034x745x822 800x500x600 1 390 0008 BF 400 400 1234x765x1022 1000x500x800 1 390 0009 BF 720 720 1234x865x1528 1000x600x1200 1 390 0010 390 2 VWR 3 j www vwr com Accessories for Incubators Cell Culture Incubators Binder Shelves for Material Pk Cat No ED FD BD KB 23 Chrome plated 1 390 6018 E B 28 Chrome plated 1 390 6000 ED FD FED BD KB M MK 53 Chrome plated 1 390 6001 ED FD FED FDL MDL BD KB KBF M 115 Chrome plated 1 390 6002 ED FD FED BD KB KBW KBWF KBF M MK 240 Chrome plated 1 390 6003 ED FED BD M 400 Chrome plated 1 390 6004 ED FED BD KB KBW KBWF KBF M 720 Chrome plated 1 390 6005 ED FD BD KB 23 Stainless Steel 1 390 6019 ED FD FED BD KB M MK 53 Stainless Steel 1 390 6006 ED FD FED FDL MDL BD KB KBF M 115 Stainless Steel 1 390 6007 ED FD FED BD KB KBW KBWF KBF M MK 240 Stainless Steel 1 390 6008 ED FED BD M 400 Stainless Steel 1 390 6010 KB KBW 400 Stainless Steel 1 390 6017 ED FED BD KB KBW KBWF KBF M 720 Stainless Steel 1 390 6009 Perforated shelves stainless steel for Pk Cat No ED FD BD KB 23 1 390 0001 E B 28 1 390 6011 ED FD FED BD KB M MK 53 1 390 6012 ED FD FED FDL MDL BD KB KBF M 115 1
56. 2 Standard Version with restricted data safety features 3 Basic Edition for simple data logging Characteristics of the GLP GMP Version of APT COM 3 DataControlSystem Matches FDA Directive 21 CFR part 11 with respect to Tel 0800 22 33 44 freephone Email uksales uk vwr com Fax 01455 55 85 86 390 3 Web http uk vwr com Cell Culture Incubators e Individual user authorisation and identification through password protected access control or electronic signature Complete and detailed Audit Trail for all system interventions e Data storage with protection against manipulation e Automatic data backup e Validability of overall system Description Pk Cat No APT COM 3 Full GMP GLP Version software 1 390 0235 APT COM 3 Standard Version software 1 390 0233 APT COM 3 Basic Edition software 1 390 0234 Incubators Natural Convection INB INE and INP Series Memmert Temperature range ambient 5 to 70 volumes 32 to 749 Stainless steel incubators with natural convection which are available in different performance classes BASIC EXCELLENT or PERFECT BASIC INB the most inexpensive models EXCELLENT INE for more exacting requirements PERFECT INP perfection in temperature control and sterilisation with Steri Card For batches that are particularly heat sensitive or valuable the EXCELLENT or PERFECT range models are ideal The incubators are equipped with large area 4 sided all ro
57. 2 sensors For an automatic CO2 control unit that is accurate and reliable there is the choice between the standard thermal conductivity sensor TC or the dual beam infrared sensor IR recommended if the temperature and humidity change frequently Shelves Inner volume WxDxH ext WxDxH int supplied Description I mm mm max Pk Cat No HERAcell 150i dual chamber stainless steel inner chamber 150 637x782x867 470x530x607 3 10 1 390 4304 complete with base frame HERAcell 150i dual chamber solid copper inner chamber 150 637x782x867 470x530x607 3 10 1 390 4305 complete with base frame HERAcell 150i st t apne Meee 150 637x782x867 470x530x607 3 10 1 390 4306 inner chamber HERA 150i solid EA T ae CORES 150 637x782x867 470x530x607 3 10 1 390 4307 inner chamber HERAcell 240i stainl teel EA iene 240 780x834x934 607x583x670 3 12 1 390 4308 inner chamber HERA 240i solid pe panes 240 780x834x934 607x583x670 3 12 1 390 4309 inner chamber 390 20 VWR F www vwr com Cell Culture Petri Dishes Petri Dishes VWRED Transparent PS w Aseptic manufactured under strict aseptic conditions using on line production and packaging Sterile irradiated with 10 kGy Proof of sterility and batch number on the box e Excellent optical quality e Stringent quality control procedures Compliance with automatic plate pourers Certificates on request
58. 21 4 3 93 114 149 0339 13 5 25 4 5 130 152 149 0264 15 25 6 6 106 131 Description Pk Cat No Fine point reticulated foam head with a blue glass filled polypropylene shaft 500 149 0343 Micro foam head open 500 149 0272 Medium pointed compressed foam head open 500 149 0271 Small foam head with flexible tip open 500 149 0268 ini pointed compressed foam head open l Small foam head open 500 149 0269 Medium flexible foam head with a nylon handle closed 500 149 0338 Cone shaped reticulated foam head with a blue glass filled polypropylene shaft 500 149 0337 Foam head with a wooden shaft 500 149 0333 Extended length reticulated foam head handle end pointed for dual use with a blue glass filled 100 149 0265 polypropylene shaft Paddle shaped foam head 500 149 0334 Medium foam head open 500 149 0267 Large flexible foam head closed 100 149 0266 Large rectangular foam head 500 149 0339 g Large rectangular foam head closed 100 149 0264 g Transport Swabs VWRE A range of collection and transport devices for bacterial specimens Available with different applicators and transport media Shipment in aluminium packaging under nitrogen ensures longer shelf life e A wide range of bacteria can survive for 24 48 hours on the applicator and in the tube e Proven in many studies with aerobic and anaerobic organisms e Each swab system is individually wrapped in a pouch with a tamper proof seal which turns
59. 331 2112 Ideal for sampling from pools tanks manholes surface water Suitable for deep and narrow shafts The telescopic rod is aluminium and can be equipped with 4 different snap on scoops for various applications The angle between the telescopic rod and beaker can be adjusted through 90 Pendulum beaker 1000 ml Irrespective of the rod angle the beaker always assumes a vertical position Bottle holder for all bottles up to 95 mm dia e Telescopic rods are fully adjustable e Pendulum beaker allows for none of the contents to be lost Description Pk Cat No Telescopic rod 0 6 1 2 m 1 300 0037 g Telescopic rod 1 3 m 1 300 0039 Telescopic rod 1 5 4 5 m 1 331 2143 Angular beaker 500 ml 1 331 2130 e Angular beaker 1000 ml 1 331 2131 e Angular beaker 2000 ml 1 331 2132 Pendulum beaker 500 ml 1 331 2120 Pendulum beaker 1000 ml 1 331 2121 Pendulum beaker 2000 ml 1 331 2122 e Bottle holder 1 331 2145 WWR ro www vwr com Sampling Samplers Fluid Sampler for Liquids LiquiSampler Sampler ViscoSampler Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Made of ultra pure and chemically inert PTFE FEP for contamination free sampling Sampling from open and closed barrels vats tanks silos open waters ponds streams lakes rivers Fits all commercially available vats and barrels with openings of at least 25 mm diameter Easy to
60. 34 0050 250 canted phenolic 60 734 0388 g 150 600 canted plug seal 40 734 0266 600 canted vented 40 734 0267 g 750 straight plug seal 40 734 0014 e 175 750 straight vented 40 734 0047 e 750 straight phenolic 40 734 0018 175 750 straight vented 40 734 0964 225 800 canted plug seal 30 734 1031 800 canted vented 30 734 0957 300 1900 straight plug seal 12 734 0039 1900 straight vented 12 734 0048 Bar coded robotics automation compatible Note culture area and capacity are nominal Cell Culture Flasks Nunclon A Nunc PS sterile Cell culture flasks with surface areas from 25 to 500 cm with filter or seme 392 1 vent close caps and straight or angled neck e Standard flasks have short wide necks for easy access e SoLo Flasks for standing cultures have a low profile design which saves incubator space four stacked SoLo Flasks occupy the same space as three conventional flasks and have a large bore angled neck which facilitates pipette and cell scraper access e TripleFlasks have the same external dimensions as a standard 175 cm flask but have three parallel growth surfaces providing a total culture area of 500 cm making them ideal for scale up e Excellent optical quality and individually leak tested e Nunclon A certified surface treatment for optimal cell growth and attachment www vwr com VWRES Cell Culture Cell Culture Flasks Standard flasks
61. 34 1904 Erlenmeyer flask Fernbach design 3L 70mm vented 4 734 1902 Aseptic Transfer Caps Transfer caps with two ports One port ends in a 0 2 um Acro 50 mm disc and the other port is C Flex Tubing ending in either a male luer lock or a male polycarbonate quick connect e Diptube reaches all the way to the bottom of the flask for easy aseptic transfer of liquid e Suitable for 1L 2L and 3L plastic Erlenmeyer flasks Description Pk Cat No 43 mm aseptic transfer cap for 1 L flask 1 8 diptube 0 2 um vent male luer lock 5 734 4209 43 mm aseptic transfer cap for 1 L flask 1 4 diptube 0 2 um vent male PC connector 5 734 4210 48 mm aseptic transfer cap for 2 L flask 1 8 diptube 0 2 um vent male luer lock 6 734 4211 48 mm aseptic transfer cap for 2 L flask 1 4 diptube 0 2 um vent male PC connector 6 734 4212 70 mm aseptic transfer cap for 3 L flask 1 8 diptube 0 2 um vent male luer lock 4 734 4213 70 mm aseptic transfer cap for 3 L flask 1 4 diptube 0 2 um vent male PC connector 4 734 4214 Replacement Caps PP Tel 0800 22 33 44 freephone Email uksales uk vwr com Fax 01455 55 85 86 Web http uk vwr com 392 36 Cell Culture Cell Culture Bottles and Tubes Replacement caps for Corning 2L and 3L Erlenmeyer flasks are available separately They are supplied sterile and individually packaged Description Pk Cat No Vent cap 48 mm for 2L flask 24 734 1215 Vent
62. 390 6013 ED FD FED BD KB KBW KBWF KBF M MK 240 1 390 6014 ED FED BD M 400 1 390 6015 KB KBW 400 1 390 0000 ED FED BD KB KBW KBWF KBF M 720 1 390 6016 Software data logging APT COM 3 DataControlSystem Binder sar ja r 4 With the APT COM 3 DataControlSystem Binder offers data communications software which fulfils all FDA regulations with respect to data safety The structure of the programme interface is user friendly and allows clear and easy recording of measurement values and data administration APT COM 3 DataControlSystem is fully network compatible All data is available at any time via intranet or internet The current process parameters can be retrieved online as HTML files Measurement values can also be looked at from home at anytime Alert messages only have to be transferred to preset telephone numbers including in the mobile network According to the computer hardware configuration with APT COM 3 up to 30 Binder laboratory instruments can be integrated and controlled using a standardised operator interface The integration of APT COM 3 under LIMS for instance with Labview is also possible The wealth of registering and monitoring functions with variable graphs allows for safe working and complete logging of measurement data APT COM 3 is available at three different performance levels 1 Full Version for applications according to FDA Directive 21 CFR part 11 in the GLP GMP range
63. 548 0717 lilac 1000 548 0715 Safe T Flex caps for 16 mm blood collecting and culture tubes green 1000 548 0729 blue 1000 548 0721 grey 1000 548 0736 yellow 1000 548 0726 Culture Tubes Corning Optically clear PS Culture tubes with threaded plug seal caps e TC treated tubes supplied racked e Untreated tubes provided bulk packed e Sterilised by gamma radiation e Certified non pyrogenic Chem_Prod_Description Pk Cat No Culture tubes 16x125 mm untreated 500 734 1697 Culture tubes 16x125 mm TC treated 500 734 1701 BD Falcon Conical Tubes with Flip Top Cap BD Biosciences PP sterile non pyrogenic The BD Falcon Conical Tube with Flip Top Cap is ideal for any research application requiring one handed operations to open and close the cap The flip top design saves time and effort in applications requiring multiple aliquoting storage and pouring from the same tube whilst maintaining the same high quality and performance of standard screw cap closures e One hand cap manipulation is virtually effortless due to the hinge action of the flip top cap and easy to read blue graduations on the tube allow rapid volumetric assessment e Splash guard design of the cap allows opening and closing without risk of splatter Click bead ensures a tight and secure seal e Ambidextrous with thumb grips on each side of the cap to ensure a sterile opening technique e Can be centrifuged up to 9400 g D
64. 58 650x470x785 2 7 1 390 0314 KBF ICH 720 700 1234x867x1816 1000x600x1168 2 14 1 390 0218 KBF LQC series Shelves Inner volume supplied Type I WxDxH ext mm WxDxH int mm max Pk Cat No KBF LQC 240 240 905x765x1458 605x470x785 2 7 1 390 0315 KBF LQC 720 700 1234x867x1816 1000x600x1168 2 14 1 390 0326 Plant Growth Chambers KBW Series Binder KBW 240 and KBW 720 temperature range 0 to 70 C without lighting KBW 400 temperature range 10 to 60 C without lighting The KBW series plant growth chambers are precise units for plant cultivation under advanced working conditions They provide perfect simulation of a wide range of growing environments The KBW plant growth chambers satisfy all requirements for optimum lighting and temperature conditions so that culture processes can be defined and reproduced exactly e Precision and outstanding dynamics e Even distribution of light on all levels e Environmental friendly e Individual programming Design Variable position daylight cassettes with 5 daylight luminescence tubes each Inner glass door access port with silicone plug 30 mm on the left hand side Shelves Supplied with 2 stainless steel shelves Safety Temperature safety device Class 3 1 with visual and acoustic temperature alarm 390 16 VWR 3 j www vwr com Cell Culture Incubators Control Microprocessor program controller with 2 programs each with 10 sections or alternatively switch over
65. 5x25 16 734 2179 Absorbent Pad Kits One handed dispensing of cellulose absorbent pads 45 5 mm Absorbent pads are ideal for absorbing broth media to culture colonies in accordance with the Membrane Filter MF Technique Enables user to dispense a clean cellulose pad into a Petri dish whenever needed without touching the pad Handy dispenser kit holds one tube of one hundred absorbent pads ten tubes included Description Pk Cat No Sterile 1000 516 9012 g Non sterile 1000 516 7850 391 6 VWRE www vwr com Cell Culture Accessories for Petri Dishes Petri Dish Racks Nalgene PC frame white PC posts For up to 72 plastic round dishes 60x15 mm Simple assembly required Smooth rounded corners e Finger grip handle and rubber feet make carrying safe and secure e Petri Dish Rack can be decontaminated by heat sterilization WxDxH 203x298x152 mm Pk Cat No 710 4106 Petri Dish Racks Nalgene Acrylic frame white PC posts For up to 54 plastic round dishes 100x15 mm or 42 glass round dishes 100x15 mm Simple assembly required e Smooth rouded corners e Finger grip handle and rubber feet make carrying safe and secure e Petri Dish Rack can be decontaminated by heat sterilization WxDxH 203x343x235 mm Pk Cat No 1 391 2161 Petri Dish Dispensers Heathrow Scientific Acrylic This sturdy dispenser will hold up to 20 90 mm Petri dishes in tw
66. 6032 regulator TC class 1 Bee temperature reguiator 28 580x425x402 400x250x280 2 4 1 390 6033 TC class 1 Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com 1 Web http uk vwr com 390 Cell Culture Incubators we ee Incubators with or without Forced Convection BD and BF eries Binder Temperature range ambient 5 to 100 C volume 20 to 720 APT line incubators distinguish themselves from all others due to their superior temperature accuracy Two incubator versions are available depending on the application The BD series with natural convection for all standard applications the BF series with forced convection for applications with high load density and particularly fast temperature recovery times The advantage of the BF series with forced convection is based on extremely precise temperature distribution within the incubator even with high load density and fast temperature recovery time after the door has been opened APT line incubators meet all quality requirements for uniform reproducible incubation conditions Their broad temperature range and high performance equipment mean that the BD and BF series can be used for all incubation tasks in research production and quality assurance e Temperature variation at 37 C 0 5 C BD series 0 4 C BF series e Additional safety is provided with disinfection at 100 C and easy operation e High degree of temperature accu
67. 75 6000 ml Celstir Magna Flex flask only 1 734 3176 8000 ml Celstir Magna Flex flask only 1 734 3177 25 ml jacketed Celstir flask only 1 734 3184 50 ml jacketed Celstir flask only 1 734 3185 125 ml jacketed Celstir flask only 1 734 3186 250 ml jacketed Celstir flask only 1 734 3187 500 ml jacketed Celstir flask only 1 734 3188 1000 ml jacketed Celstir flask only 1 734 3189 Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 46 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Tissue Culture Flasks Siliconised borosilicate glass For use with the MCS platform biological stirrer ideal for suspension cell culturing with reduced cell attachment e Incorporate a unique base design which together with the bulb ended stirrer ensures that cultures are lifted into suspension at the lowest possible speeds preventing cell damage e Can be sealed for use with pathogenic materials e Culture vessels are siliconised to reduce the possibility of cells attaching to and growing on the surfaces Capacity ml Neck Pk Cat No 125 Straight 1 442 0706 500 Straight 1 734 0880 Shaft Assembly Kit for Celstir Flasks Wheaton A non breakable stainless steel shaft to replace the glass shaft which is included with Celstir flasks May be retrofitted to existing Celstirs Description Pk Cat No For 25 ml flask 1 734 3150 For 50 ml flask 1 734 3151 For
68. 90 0358 Cooled Incubators ICP Series Memmert Temperature range 0 to 60 C volumes 53 to 749 Compressor cooled incubators with finely tuned control technology Memmert s PERFECT controller which ensures deviations from the setpoint are minimised guaranteeing the safety of temperature sensitive living cultures at all times Ideal for use where rapid and precise changes between heating up and cooling down phases in ramp operation are called for e Air jacketted heating system rapid and precise temperature control no possibility of the load drying out as the thermal jacket is separated from the interior so the chamber does not dehumidify e Integrated timer for temperature profiles of up to 40 ramps each segment adjustable from 1 min up to 999 h e Fan speed can be controlled and set in increments of 10 from 10 to 100 via controller e Safety features include the Automatic Safety Function for over and under temperature automatically follows the setpoint in a selectable tolerance range protection Class 3 3 e Autodiagnostic system with fault indication and highly efficient automatic defrosting system Design Units have easy to clean stainless steel interior with deep drawn ribbing inner glass door and stainless steel outer shell Shelves 2 perforated stainless steel shelves choice of 4 to 14 shelf positions depending on size of incubator Cleaning and disinfection Corrosion resistant stainless steel chamber and
69. 91 2019 Deep single vent 50 20 3 500 391 2022 4 Shallow single vent 52 14 5 700 391 2020 No vent 55 5 12 1620 391 2041 Triple vent 55 5 12 1620 391 2042 Single vent 60 15 1 540 391 2021 Contact plate 67 10 5 300 391 2031 Single vent 89 2 16 500 391 2015 Triple vent 89 2 16 2 500 391 2016 3 Triple vent 2 compartments 89 2 16 2 391 2029 Triple vent 3 compartments 89 2 16 2 500 391 2030 quare x mm 5 Repli Square 100x100 mm Repl tong 208 120 M dish with 25 compartments Triple vent 138 9 21 1 80 391 2028 The quick and easy way to request a quote Just e mail quote me uk vwr com Tel 0800 22 33 44 freephone Email uksales uk vwr com 391 3 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Petri Dishes Petri and Contact Dishes Nunc Petri Dishes Steriplan DURAN Group Petri Dishes Contact Plate 391 4 Optically clear polystyrene Contact dish with printed grid useful for sampling in hospital environments as well as in food science and the pharmaceutical industry e Ideal for automated systems e Radiation sterilised Height Version ae mm _ mm Sterile Pk Cat No tact H act dish for 145 21 180 710 2510 E tact dish ith con act dishes wi 67 15 396 710 2507 grid for 7 7 ml Petri dish vented for 100 15 320 391 8020 z 12 5 ml Soda lime glass e Bottom dish and lid inside and outside microscopic flat free of bubbles and
70. C 200 litre ambient 8 to q 70 C volume 50 to 200 A range of efficient reliable cost effective incubators ideal for biological analysis research and general laboratory applications e Fluctuation 0 25 C at 37 C J e 200 litre model has fan assisted circulation other models shown are supplied with natural convection but are available with optional fan e Temperaure range is ambient 8 for 200 model and ambient 5 for all other models e Units have double door solid outer clear glass inner and microprocessor digital controller which displays both set and actual temperature e Microprocessor digital controller is configured for each individual unit to optimise the heat up minimise the overshoot and control of the temperature Design There are two designs INC and MINI to facilitate operation depending on the location of the incubator The INC has a horizontal style with the controls fitted to one side of the door The MINI has a vertical style where the controls are fitted below the door near the base and would be less suitable for underbench locations All the models listed below feature an exterior of sheet steel finished in an easy clean powder coated paint a stainless steel inner chamber and double doors comprising steel outer with an inner glass door for easy sample viewing Units are heated by means of Incoloy sheathed elements positioned below the chamber floor for natural convection units and fitted around the fan on
71. CI 200 200 530x660x1570 420x380x1300 1 390 0333 C1 280 280 640x640x1900 440x410x1620 1 390 0334 Cooled Incubators Series 3 Temperature range 10 to 50 C volumes 90 to 450 Series 3 cooled incubators are temperature controlled cabinets with fan assisted air circulation via a pre mixing chamber They are ideal for studying growth patterns of plants and insects BOD tests seed germination tissue culture enzyme testing and fruit fly culture e Units feature automatic defrost e Easy to use controls with digital display of actual temperature e Hermetically sealed refrigeration system and suppressed RF and TV electronics e Full PID heating control to ensure stable internal temperature Design The units have a white enamelled sheet steel exterior stainless steel lined interior with polyurethane foam insulation and feature a door lock and magnetic door gasket Shelves Supplied with white plastic coated wire shelves and self evaporating condensate drip tray Cleaning Corrosion resistant inner chamber is easy to clean disinfection procedures are detailed in the user manual Safety Features include variable over temperature alarm safety cutout preset at 70 C and refrigeration isolation switch Controller Controls are recessed in the control panel to avoid accidental alteration Full PID heating control indicator for temperature overheat alarm Connections 230 V 50 60 Hz RS 232 interface Technical data Average temper
72. Can be sterilised e Very sharp e Complete with case Description Pk Cat No Ice borer complete 1 312 0072 Ice Sampler screws into the sample material and simultaneously extracts and conveys the sample into the sampling cylinder The Sampling cylinder is detachable The Ice Sampler is electropolished and does not have any flutes for perfect and easy cleaning Developed for sampling from frozen materials and similar substances The e Sterilisable e Electropolished e Simple cleaning Description Pk Cat No Ice Sampler complete 300 mm 1 300 1047 Ice Sampler complete 550 mm 1 300 1048 Ice Sampler complete 1050 mm 1 300 1049 Accessories Description Pk Cat No Transport case 300 mm 1 300 1050 Transport case 500 1050 mm 1 312 0071 VWR E53 www vwr com Sampling Samplers Solid Control Seal close it In contrast to normal labels close it adheres well to sacks that are coated with powder plaster flour etc close it is a self adhesive seal with aluminium backing which adheres very firmly Sacks cartons etc which have been pierced and sampled by Powder Proof or other samplers can be hermetically re sealed The sampling date the name of the quality supervisor and other information can be written on the seal with pen pencil or felt tip pen 1 roll contains 500 stickers e Adheres very firmly e Can be written on e Size 95x95 mm Description Pk Cat No Printed
73. D Cell Tak is used to attach cells or tissue sections to many types of surfaces including plastic glass metal Teflon and biological materials It can simplify the manipulation of biological samples for a number of in vitro techniques including in situ hybridization immunoassays microinjection immunohistochemistry and establishing primary cells in culture BD Cell Tak is biocompatible and demonstrates no species specificity Formulation As a liquid in 5 acetic acid Source Polyphenolic proteins secreted by Mytilus edulis Dopa protein ratio gt 0 05 Quality control Tested for ability to promote cell attachment and spreading of BHK 21 and U937 cells Tested and found negative for bacteria fungi and mycoplasma Molecular weight 110 140 kD Storage and stability Stable for at least three months at 2 8 C Do not freeze Description Pk Cat No 1 mg 734 1081 BD Cell Tak cell and tissue adhesive 5 mg 734 1083 10mg 734 0102 BD Matrigel Matrix High Concentration BD Biosciences BD Matrigel Matrix High Concentration HC is suited for in vivo applications where a high protein concentration augments growth of tumours The high protein concentration also allows the BD Matrigel Matrix plug to maintain its integrity after subcutaneous injection into mice This keeps the injected tumour cells and or angiogenic compounds localized for in situ analysis and or future excision Applications include in vivo
74. F and TV electronics e Full PID heating control to ensure stable internal temperature Shelves supplied Type Inner volume I __WxDxH ext mm WxDxH int mm max Pk Cat No 1200 NP 1200 1370x800x2000 1240x670x1460 10 48 1 390 0084 600 NP 600 690x800x2000 540x670x1460 5 24 1 390 0100 Please note that with the maximum number of shelves fitted in a cabinet there is only a 20 mm gap between each shelf Optional Extras for LMS Cooled Incubators These items must be ordered with the appropriate incubator model and cannot be retrofitted Description for Pk Cat No Castors Series 4 all models 1 390 0104 Internal socket All models 1 390 0125 Access port 38 mm All models 1 390 0103 Chart recorder All models 1 390 0105 Double glazed doors Description for Pk Cat No Double glazed door Series 3 model 400 1 390 0360 Double glazed door Series 3 model 200 1 390 0109 Double glazed door Series 3 model 100 1 390 0110 Drosophila modification For application involving Drosophila a modification for aggressive environments will be required This factory fitted option cannot be retrofitted Please contact us before ordering your incubator 390 14 VWR 3 j www vwr com Cell Culture Incubators Description for Pk Cat No Drosophila modification Series 3 model 100 1 390 0374 Drosophila modification Series 3 model 200 1
75. High quality medical grade polypropylene ensures all swab tubes are non toxic and have a low moisture permeability Every swab has a tamper evident seal and is printed with lot number expiry date and full description e Supplied sterile Description Pk Cat No Albumin coated 100 720 0059 Plain swab wood shaft bulk 2500 720 0025 Plain swab twisted wire shaft 100 720 0063 e business more than just a webshop Requisitioning approval workflows order transmission advance shipping notice order tracking goods receipt and invoicing are just a few areas where VWR International can benefit our customers through B2B This will enable streamlined efficient process flows for both our customers using vwr com as well as those utilising E Procurement tools or third party software solutions Please visit us at http eu vwr com e business i 300 17 VWR E53 www vwr com Incubators Incu Line INCU Line Cell Culture Incubators VWRED yy Temperature range ambient 5 to 70 C volumes 53 and 115 Incubator with natural air circulation for all standard incubation applications e Micro processor control with large digital temperature display e Temperature setting in increments of 0 1 C e Built in timer from 0 999 minutes or 00 0 99 9 hours or continuous mode e Protection against overheating with a visual alarm safety thermostat K 3 1 e Ventilation duct on the back of the unit
76. Large cell scraper 30 mm blade 390 mm handle 100 734 1528 Plate Roller for Securing Films and Foils to Microplates VWR CF Soft rubber roller recommended for assuring tight and consistent application F of adhesive films to microplates Description Pk Cat No Plate roller 1 391 1278 392 55 VW R E F www vwr com Cell Culture Cell Culture Accessories and Sealing Systems Plate Paddles for Securing Films and Foils to Microplates VW R E 3 7 An alternative to the plate roller for pressing films to assure a secure uniform seal around all wells The paddles are especially recommended for sealing films on raised rim plates because they fit within the rim of the plate Description Pk Cat No Film sealing paddles 50 391 1283 Well Identification Labels for 96 Well Flat Bottom Plates VWR Cz Handy labels for application on the underside of flat bottom 96 well plates Each well is identified by a black on clear alphanumeric index that can be easily captured in microscopic images Label positions easily by alignment with inside of plate edges e Identify wells clearly when pipetting e Identify wells under microscopic examination e Position easily on underside of standard 96 well plates Description Pk Cat No Plate bottom labels non sterile 100 391 1284 Plate bottom labels sterile 50 391 1285 Breatheable Rayon Films for Biological Cultures VWRE A 114 um thick hydrophobic porous film with medica
77. PE screw cap closure The caps for the 30 1000 ml bottles are a natural off white colour The cap for the 2000 ml bottle is white Bottles and closures are not autoclavable Packaged in shrink wrapped trays e Leak proof break resistant and durable e Transparency and reduced permeability to carbon dioxide and oxygen allow media storage up to 6 months Bottles and closures are radiation sterilised and non pyrogenic Description Capacity ml WxDxH mm Neck I mm Pk Cat No 30 38x38x64 14 280 216 0306 60 41x41x83 18 200 216 0307 125 54x54x110 28 96 216 0308 Bottles with screw caps 250 61x61x146 28 60 216 0309 500 74x74x177 28 40 216 0310 1000 94x94x220 28 24 216 0311 2000 116x116x171 39 12 216 0312 30 38x38x64 14 280 216 0379 60 41x41x83 18 200 216 0380 125 54x54x110 28 96 216 0381 Bottles without screw caps 250 61x61x146 28 60 216 0382 500 74x74x177 28 40 216 0383 650 82x82x180 28 24 216 0384 1000 94x94x220 28 48 216 0385 Media Bottles Square Sterile Wheaton PET bottle with white PE cap seme These bottles offer the clarity of glass the strength of plastic and a space saving design e Tested for pH stability temperature durability cloning efficiency and cytotoxicity e No drip pour lip allows cleaner faster and easier pouring e Permanent in mould graduations provide volume determination at a glance e Lightweight for more economical shipping and handling e 20
78. PI infrared measuring system without drift error Entirely condensation free even at high humidity Design Lockable door with choice of left or right hand side hinges Weldless deep drawn inner chamber with integrated shelf support system Shelves Supplied with 3 perforated stainless steel shelves Cleaning and disinfection Hot air sterilisation at 180 C complies with DIN 12880 units have an easy to clean interior without fan or HEPA filter Safety Automatic diagnostic system with optical and acoustic alarm as well as zero voltage relay contact for central monitoring locking controller keyboard via 3 digit password temperature safety device class 3 1 Control Air jacket system microprocessor control for temperature and CO2 with various alarm and status displays built in water condensation control Technical data Temperature fluctuation 0 1 C Connections 230 V 50 60 Hz 1 2 kW Calibrations and validations possible Supplied with Binder test certificate Shelves Inner supplied Type volume I WxDxH ext mm WxDxH int mm max Pk Cat No C 150 right hinged door 150 680x832x819 500x500x600 3 6 1 390 6068 C 150 right hinged door stacking set of 2 j 2x150 680x815x1783 500x500x600 3 6 1 set 390 6070 incubators with stacking adapter C 150 left hinged door 150 680x832x819 500x500x600 3 6 1 390 6069 C 150 left hinged door tacki tof2 B 2x150 680x815x1783 500x500x600 3 6 1set 390 6071 incu
79. Pk Cat No Inserts for 6 well plates 48 734 0032 g Inserts for 12 well plates 48 734 0051 Inserts for 24 well plates 48 734 0036 High density translucent PET membrane 0 4 um pore size 1 0x10 pores cm Description Pk Cat No Inserts for 6 well plates 48 734 0061 Inserts for 12 well plates 48 734 0062 Inserts for 24 well plates 48 734 0063 Transparent PET membrane 1 0 um pore size 1 6x10 pores cm Description Pk Cat No Inserts for 6 well plates 48 734 0040 Inserts for 12 well plates 48 734 0041 Inserts for 24 well plates 48 734 0042 Transparent PET membrane 3 0 um pore size 8 0x10 pores cm Description Pk Cat No Inserts for 6 well plates 48 734 0033 Inserts for 12 well plates 48 734 0052 Inserts for 24 well plates 48 734 0037 High density translucent PET membrane 3 0 um pore size 2 0x10 pores cm Description Pk Cat No Inserts for 6 well plates 48 734 0034 Inserts for 12 well plates 48 734 0060 Inserts for 24 well plates 48 734 0397 Transparent PET membrane 8 0 um pore size 1x10 pores cm Description Pk Cat No Inserts for 6 well plates 48 734 0035 Inserts for 12 well plates 48 734 0053 Inserts for 24 well plates 48 734 0038 392 29 VWRE www vwr com Cell Culture Cell Culture Inserts BD BioCoat Collagen and Collagen IV Cell Culture Inserts BD Biosciences BD Falcon Cell Culture Inserts coated wit
80. Polycarbonate membrane 0 4 um pore size 1 5x10 pores cm Recommended Description working volume ml Culture area cm Pk Cat No Inserts for 6 well multidishes 25 mm 1 75 4 2 48 734 2016 rt pack well multidish Inserts pre packed in 6 well multidishes 20x25 15 Si m 734 2240 mm rt packed in 12 well multidishes 12x1 da s pre packed in 12 well multidishes 12x18 14 1 13 48 734 2232 rt pack 24 well multidish 1 ka s pre packed in 24 well multidishes 8x13 05 0 47 48 734 2239 rt pack well multidish 4 pe s pre packed in 6 well multidishes 23x3 4 78 A 24 734 2235 Inserts for 24 well multidishes 10 mm 0 5 0 5 48 734 2015 Polycarbonate membrane 3 0 um pore size 3x10 pores cm Recommended Description working volume ml Culture area cm Pk Cat No Inserts for 6 well multidishes 25 mm 1 75 4 2 48 734 2020 rt pack well multidish 4 a s pre packed in 6 well multidishes 23x3 478 iA gt A 734 2236 Inserts for 24 well multidishes 10 mm 0 5 0 5 48 734 2019 rt pack well multidish js s pre packed in 6 well multidishes 20x25 15 3 14 gt A 734 2230 rt packed in 12 well multidishes 12x1 fia s pre packed in 12 well multidishes 12x18 14 1 13 48 734 2233 rt pack 24 well multidish 1 Inserts pre packed in 24 well multidishes 8x13 05 0 47 48 794 2228 mm Polycarbonate membrane 8 0 um pore size 10 pores cm Recommended Description workin
81. Recommended Growth area cm working volume ml Neck Cap Pk Cat No 25 7 angled filter 160 734 2004 angled vent close 160 734 2081 80 30 straight filter 50 734 2131 straight vent close 50 734 2046 175 68 straight filter 32 734 2129 straight vent close 32 734 2067 SoLo flasks Recommended Growth area cm working volume ml Neck Cap Pk Cat No 185 75 angled vent close 50 734 2034 angled filter 50 734 2035 Triple flasks Recommended Growth area cm working volume ml Neck Cap Pk Cat No 500 200 straight vent close 32 734 2000 e straight filter 32 734 2001 e Caps for Nunclon A flasks HDPE sterile Description Pk Cat No Filter caps for 25 cm flasks 100 734 2159 Filter caps for 175 185 500 cm flasks 100 734 2153 Vent close caps for 175 185 500 cm flasks 100 734 2152 Cell Culture Flasks EasYFlasks Nunclon A Nunc PS sterile Designed to allow full access to the growth surface e Flask is opened or closed with turn of the cap e Y Mark caps allow visual verification of vent position even when stacked in incubators e Volume graduations on both sides of the flask e Angled neck facilitates easy access e Nunclon A certified surface treatment for optimal cell growth and attachment Recommended Growth area cm working volume ml Neck Cap Pk Cat No 25 7 angled vent close 200 734 2063 angled filter 200 734 2064 75 30 angled vent close 100 734 2
82. Safety Features include independent over temperature safety protection Controller Digital indicator and keypad settings are maintained if power is interrupted Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No Roll In CO incubator UK plug 1132 1042x864x2210 890x660x1930 1 390 0165 Roll In standard incubator UK plug 1132 1042x864x2210 890x660x1930 1 390 0163 Shelves Description for Pk Cat No Shelf Roll In incubators 1 390 0164 Refrigerated Incubators KB Series Binder Temperature range 5 to 100 C volumes 20 to 700 Refrigerated incubators are mainly used for culturing tasks that need to be carried out at temperatures below room temperature or if high ambient temperatures cannot be avoided The DCT cooling system in conjunction with the APT line temperature technology provides ideal conditions assuring temperature accuracy and reproducible results in both heating and JA cooling situations with minimal dehumidification of specimens and impressive performance The DCT cooling system and the controllable fan guarantee high degrees of humidity even during cooling mode or prolonged testing to protect samples from drying out e APT line preheating chamber technology e Adjustable fan speed aA e Foam insulation contains no FCHCs environmentally friendly refrigerant R 134a e Temperature deviation 0 2 C at 25 C Design Internal glass door Units up to 115 litres can be stacked o
83. Slide 5 0 6 5 8 6 24 734 0086 1 Well CultureSlide 5 0 6 5 8 6 96 734 0399 2 Well CultureSlide 2 0 2 5 4 07 24 734 0087 2 Well CultureSlide 2 0 2 5 4 0 96 734 0400 Well Cultureslide O 1 l 4 Well CultureSlide 1 0 1 5 1 7 96 734 0401 8 Well CultureSlide 0 8 0 8 0 7 24 734 0089 8 Well CultureSlide 0 8 0 8 0 7 96 734 0402 per well Chamber Slides BD BioCoat Collagen I CultureSlides BD Biosciences Soda lime glass slide PS vessel lid and tool Collagen is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture In vitro use of collagen can exert effects on the adhesion morphology growth migration and differentiation of a variety of cell types BD BioCoat Collagen CultureSlides allow cells to be cultured and then analysed on a glass microscope slide Cells are grown in a plastic chamber attached to a specially prepared microscope slide Cells can be fixed and stained in situ without disruption of the cell monolayer Chamber is easily and safely removed using the disposable safety removal tool supplied e Pressure sensitive biocompatible acrylic adhesive gasket remains with the vessel after removal not on the slide facilitating further processing or placement of coverslips e Blue hydrophobic border defines cell culture areas e Wells numbered for easy identification e Trays designed for incubator use Slide
84. Storage and stability Stable for at least three months at 70 C Description Pk Cat No Extracellular Matrix human 1mg 734 0097 BD 3 Dimensional Scaffolds BD Biosciences A variety of 3D biodegradable scaffolds are used as artificial substitutes for the extracellular matrix These materials may consist of natural molecules and or synthetic polymers In contrast to conventional 2D cell culture systems 3D scaffolds provide an adhesive substrate that also serves as a 3D physical support matrix for in vitro cell culture as well as in vivo tissue regeneration BD 3D Calcium Phosphate BD 3D Collagen Composite Type BD 3D OPLA Scaffold Scaffold Scaffold Scaffold xh mm 5x3 fit into well of a 96 well plate 5x3 fit into well of a 96 well plate 5x3 fit into well of a 96 well plate Volume cm 0 058 0 039 0 039 Hydration capacity ul 30 25 30 Average pore size um 200 400 100 200 100 200 Propert Sponge like structure non Sponge like Sponge like structure non pony compressible structure compressible compressible ag Stable for at least 12 months Stable for at least 12 months Stable for at least 12 months Storage and stability when stored at 4 30 C when stored at 4 30 C when stored at 4 30 C Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 62 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Media and Supplements BD 3D Calcium Phosphate Scaffo
85. Temperature Incubator Heraeus BK 700 Thermo Scientific Temperature range 3 to 40 C volume 190 For incubating cultures at low temperatures e Electronic 3 point thermostat e Condensation free inner chamber e Integral timer Design The inner housing is made from impact resistant plastic A magnetic strip ensures easy opening and closing of the cabinet door Soft Touch control panel with digital temperature display and status display for cooling heating and alarm This incubator is designed for using magnetic stirrers or other equipment inside the unit Control An integral timer 1 9999 min to 7 days switches on 2 sockets located in the inner chamber and enables cyclical and reproducible tests with accessories such as magnetic stirrers 3 Point temperature controller Connections 230 V 50 60 Hz 230 W 2 sockets in the usable space Technical data Temperature deviation temporal 1 C spatial 1 C cooling time from 25 to 5 C 48 minutes weight 46 kg Shelves supplied Type Inner volume I WxDxH ext mm __ WxDxH int mm max Pk Cat No BK 700 190 540x560x1295 440x435x995 3 14 1 392 0400 Cooled Incubators Genlab 390 0332 390 0335 Temperature range 2 to 50 C volumes 50 to 280 Range of accurate efficient and economical cooled incubators that are ideal for a wide range of research testing and general laboratory applications e All units have fan assisted air circulation for even tempera
86. The basic unit consists of a container with a single 600 mm shaft A second shaft 331 0005 can be screwed onto the handle for more length e Very good chemical resistance e Temperature resistant suitable for use at high and low temperatures 200 to 280 C Description Capacity ml Pk Cat No Dipper 54 mm 100 1 331 0006 e ipper mm z z Dipper 80 mm 500 1 331 0008 e Dipper 100 mm 1000 1 331 0009 e Replacement extension shaft 7 331 0005 Tel 0800 22 33 44 freephone Email uksales uk vwr com 300 4 Fax 01455 55 85 86 Web http uk vwr com Sampling Samplers Fluid Sample Dippers 331 2130 331 2120 331 2145 VWRED All sample dippers are made of blue polypropylene and consist of a bottle with snap handle Available with either hinged or screw cap e Containers designed for sample collection and dispatch for testing in the same container eliminating cross contamination risks seme e Gamma sterilised and individually packed in sealed zip bag to guarantee integrity of sample Description Capacity ml Pk Cat No 40 250 331 3414 Screw cap 125 100 331 3416 180 100 331 3415 g Hinged cap 90 150 331 3413 For aggressive liquids with fixed rod parts coming into contact with sample media made of PP Three sizes e Rod length 100 cm e Without metal Description Capacity ml Pk Cat No 250 1 331 2110 g Chemistry Scoop 500 1 331 2111 g 1000 1
87. a needle for sample collection or infusions even while it is in use e Cost efficient can be used repeatedly e Available in 2 sizes to fit a variety of culture flasks Description for mm Pk Cat No BugStopper 28 10 516 1720 28 100 516 1721 37 10 516 1722 BugStopper 10 37 50 391 0148 391 12 VWR 3 j www vwr com Cell Culture Stoppers LABOCAP Caps Aluminium Without handle Cap for sterile but not hermetic closing of test tubes e Made of aluminum anodized with inner stainless steel spring e Springs clamp against the outer wall of the vessel e For sterile non hermetic sealing of vessels e Anodised and coloured e Autoclavable Description for mm Pk Cat No 12 13 10 391 5901 g LABOCAP silver 15 16 10 SEIREUN 19 20 10 391 5913 g 12 13 10 391 5902 LABOCAP slu 17 18 10 391 5911 12 13 10 391 5903 b LABOCAP red 14 15 10 391 5906 17 18 10 391 5912 e LABOCAP Caps Aluminium With handle Cap for sterile but not hermetic closing of test tubes e Made of aluminum anodized with inner stainless steel spring e Springs clamp against the outer wall of the vessel e For sterile non hermetic sealing of vessels e Anodised and coloured e Autoclavable Description for mm Pk Cat No 12 13 10 391 5921 14 15 10 391 5924 LABOCAP silver 15 16 10 391 0094 g 17 18 10 391 5930 19 20 10 391 5933 LABOCAP blue 17118 10 391 5931 g
88. accurate cooled incubators are designed for use in the temperature range from 5 to 60 C The forced air circulation in the interior is created by the Peltier circulation fan All process functions are visible additional language selection via set up is possible All relevant data is logged for the long term by a 1024 kB ring buffer serving as a data logger Multiple over temperature protection is provided by an audible and visual alarm USB serial interface with Celsius programming and logging software and a 32 kB MEMory card are also included The ramp timer is designed for 40 variable ramps each from 1 min to 999 h The glass operating panel has a multifunction wideband display and input module e Maximum safety for sensitive samples unsurpassed environmental features e Adaptive fuzzy supported multifunctional PERFECT PID process controller e Energy consumption reduced by up to 90 no cooling medium no refrigerant waste and minimal noise e Safety features include the Automatic Safety Function for over and under temperature automatically follows the setpoint in a selectable tolerance range protection Class 3 3 Condensation which is physically unavoidable forms outside in the cooling element not in the interior no dripping water Design Units have easy to clean stainless steel interior with deep drawn ribbing inner glass door and stainless steel outer shell Shelves IPP 200 and IPP 300 have 1 other units suppli
89. ace has a protein like structure Cultivation of cells in serum reduced media is possible cellGrade plus surface is suitable for the cultivation of more fastidious cell lines Description Colour Culture area cm Well volume Pk Cat No Standard plate with F bottom transparent 0 32 350 ul 50 735 2116 Standard plate with F bottom white 0 32 350 ul 50 735 2117 Standard plate with F bottom black 0 32 350 ul 50 735 2118 Transparent base with F bottom white 0 31 330 ul 50 735 2119 Transparent base with F bottom black 0 31 330 ul 50 735 2120 cellGrade premium This surface is a Poly D Lysine equivalent With the optimised surface characteristics the most fastidious cell lines can be cultivated Cells show growth comparable to Poly D Lysine surfaces 392 11 VWR 3 j www vwr com Cell Culture Cell Culture Plates and Dishes Description Colour Culture area cm Well volume Pk Cat No Standard plate with F bottom transparent 0 32 350 ul 50 735 2124 Standard plate with F bottom white 0 32 350 ul 50 735 2125 Standard plate with F bottom black 0 32 350 ul 50 735 2126 Transparent base with F bottom white 0 31 330 ul 50 735 2127 Transparent base with F bottom black 0 31 330 ul 50 735 2128 inertGrade Surface inhibits cell binding Especially suited for the cultivation of cells when adhesion is not desired for example when working with cell suspensions or stem cells Description Co
90. actor Reduced GFR product is useful where a more highly defined basement preparation is desired Source Engelbreth Holm Swarm mouse tumour Tested for ability to gel quickly and maintain this form with culture medium for a period of 14 days at 37 C Tested for the ability to promote neurite outgrowth of chick dorsal root ganglia cells Tested and found negative for bacteria fungi and mycoplasma Tested for endotoxin LAL assay Quality control Storage and stability Stable for at least three months at 20 C or 12 days at 37 C Description Pk Cat No BD Matrigel Basement Membrane Matrix 10 ml 734 1100 Growth factor reduced BD Matrigel Matrix 10 ml 734 0269 BD Human Extracellular Matrix BD Biosciences Human Extracellular Matrix ECM is a chromatographically partially purified matrix extract derived from human placenta It is comprised of laminin collagen IV and heparan sulphate proteogycan Human ECM promotes attachment spreading mitosis and differentiation of anchorage dependent epithelial cells particularly of human origin Formulation Frozen in 20 mM sodium phosphate buffer pH 7 4 Source Human placenta Partially purified Tested for ability to initiate differentiation of NG 108 human neuroblastoma cells Filtered and tested and found negative for bacteria fungi and mycoplasma Source material tested for hepatitis B antigen and HIV 1 antibody Quality control
91. ange 5 12x75 1000 212 9601 PS culture tubes natural 8 13x100 1000 212 9605 PP culture tubes natural 8 17x100 1000 212 9597 e PS culture tubes natural 14 17x100 1000 212 9596 Bioreactors Disposable SuperSpinner D1000 Sartorius Stedim Biotech g use bioreactor for efficient lab scale cultivation of animal cells The main feature of the SuperSpinner D 1000 is a membrane stirrer which allows controlled and gentle mixing bubble free aeration and avoids foam generation Applications include lab scale production of recombinant proteins monoclonal antibodies and biomass as seed culture the cultivation broth can be transferred directly from the SuperSpinner D 1000 into a larger bioreactor Recommended working volume 200 800 ml per flask The SuperSpinner D 1000 is a fully disposable pre assembled and ready to seme The SuperSpinner D 1000 consists of a cultivation flask and a membrane aeration system that also functions as a stirrer A hollow fibre membrane is wound around the stirrer bar which contains a magnetic core driven by a magnetic drive unit A membrane gas pump feeds ambient air through a sterile filter into the flask e Fully pre assembled disposable sterile and ready to use e High cell densities can be achieved due to the efficient membrane gassing and agitation system e A clave adapter which fits to common laboratory syringes ensures safe and easy sampling in place during cultivation e Feeding with su
92. ariety of cell types Lyophilised from CAPS buffer containing NaCl and CaCl2 pH 11 0 reconstitute in distilled water do not agitate or swirl Source Human plasma Quality controlled by SDS PAGE and tested and found negative for bacteria fungi and mycoplasma Source material tested for hepatitis B antigen and HIV 1 antibody 440 kD unreduced form Stable for three months at 2 8 C lyophilised or two weeks at 20 C solubilised Do not store in frost free freezer Avoid multiple freeze thaws Formulation Quality control Molecular weight Storage and stability Description Pk Cat No 1 mg 734 0085 Fibronectin human 5 mg 734 0101 25mg 734 0103 BD Poly D Lysine BD Biosciences Poly D Lysine PDL is a synthetic molecule used as a coating to enhance cell attachment to plastic and glass surfaces It has been used to culture a wide variety of cell types particularly neurons glial cells and transfected cells Formulation Lyophilised from aqueous solution reconstitute in distilled water Source Synthetic Quality control Quality controlled by SDS PAGE and tested and found negative for bacteria fungi and mycoplasma Molecular weight 500 550 kD Storage and stability Stable for at least three months at 2 8 C lyophilised or one week at 2 8 C solubilised Description Pk Cat No Poly D Lysine 20 mg 734 1102 Tel 0800 22 33 44 freephone
93. asy to use and ready to use straight from the box no accessories needed e High yield and process efficiency e Nunclon A surface treatment to promote consistent performance for cell attachment and proliferation in serum free and serum containing media WXxL 250x335 mm Delivery Information Shipped sterile and ready to use for single use applications Email uksales uk vwr com 2 52 Web http uk vwr com 392 5 Cell Culture Cell Culture Larger Scale Bioprocessing Recommend Growth area ed working Type cm volume ml Pk Cat No 1 Level 6302 200 6 734 2242 2 Levels 1260 400 6 734 1443 4 Levels 2520 800 4 734 1445 10 Levels 6300 2000 2 734 1444 g Accessories Description Pk Cat No PC connector 10 390 0343 Gelman filter with connector sterile 2 390 0344 0 2 um air filter with connector sterile 2 390 0345 Cell Factories Nunclon A Nunc PS sterile For the industrial scale production of for example vaccines monoclonal antibodies or pharmaceuticals e Ideal for adherent cells but can also be used for suspension cultures e Growth kinetics are unaltered from laboratory scale culture e Available in 1 2 10 and 40 tray versions for easy scale up e Low contamination risk e Certified Nunclon A surface treatment ensures excellent conditions for cell attachment and growth Tray LxW 335x205 mm Recommended working Type volume ml Growth area cm Pk
94. ated 100 734 1619 Black plate with clear bottom poly D lysine coated 100 734 1620 Plates Collagen I Coated Nunc PS ready to use plates pre coated with Collagen I with lid e Consistent growth surface from each lot ensures guaranteed performance e High quality surface promotes cell attachment growth and differentiation in serum free and serum containing media e Stable at room temperature WXxL 128x86 mm Description Colour Pk Cat No transparent 20 734 1185 MicroWell plate F96 high flange design black 20 734 1183 white 20 734 1187 6 Well multidish flat bottom transparent 20 735 0226 384 Well optical bottom plate pinchbar design black 20 734 1188 Plates Poly D Lysine Coated Nunc PS ready to use plates pre coated with Poly D Lysine with lid e Consistent growth surface from each lot ensures guaranteed performance e High quality surface promotes cell attachment growth and differentiation in serum free and serum containing media e Stable at room temperature WXxL 128x86 mm Description Colour Pk Cat No transparent 20 734 1186 MicroWell plate F96 high flange design black 20 734 1184 white 20 734 1177 392 19 VWRA 25 doy www vwr com Cell Culture Cell Culture Plates and Dishes BD Falcon Cell Culture Dishes BD Biosciences PS sterile with lid Designed for cell culture these dishes have a uniform surface chemistry created by vacuum gas plas
95. ature variation of 0 5 C and fluctuation of 0 1 C with an average load Heating time 20 to 45 C in 30 min cooling time 20 to 0 C in 50 min subject to load and ambient temperature Calibrations Optional conformance certificate and on site UKAS Accreditation of laboratory equipment are available Please enquire for details Accessories Extensive range of accessories and factory fitted options such as interior programmable fluorescent UV lighting inner perspex door manual high low temperature alarms audible and visible with safety cut out built in chart recorder RS485 interface and access ports are available on request please enquire for further details Please note if application involves Drosophila a modification for aggressive environments is required please contact us before placing your order Fully programmable models are available Inner Shelves supplied Type volume I WxDxH ext mm WxDxH int mm max Pk Cat No 100W 90 610x600x810 540x440x490 2 5 1 390 0081 200W 227 680x570x1470 550x450x920 3 20 1 390 0085 300W 290 680x570x1780 550x450x1 170 3 25 1 390 0089 400W 450 680x860x1780 550x700x1170 3 25 1 390 0096 Dual temperature cycling models are avaialble please enquire Internal dimensions shown above are maximum dimensions internal usable space will be less due to space occupied by refrigeration system Please note that with the maximum number of shelves fitted in a cabinet there i
96. bators with stacking adapter Tel 0800 22 33 44 freephone Email uksales uk vwr com 390 19 Fax 01455 55 85 86 Web http uk vwr com Cell Culture CO Incubators CO Incubators HERAcell i Series Thermo Scientific Ps Temperature range Ambient 3 to 55 C volume 150 or 240 COz incubators which provide an ideal in vitro environment clean reliable and user J friendly for the protection of valuable samples and for optimal cell growth The 4 HERAcell i series CO2 incubators provide stable accurately monitored growth conditions offering unsurpassed protection against contamination for valuable cell and tissue cultures Extremely short recovery times enhance cell growth They are available in two practical sizes and two inner chamber designs non corrosive stainless steel or 100 antimicrobial solid copper ee a e Optimum growth cultures thanks to secure and stable incubation conditions e Outstanding permanent protection from contamination saves time and prevents the loss of cultures e Reliable contamination thanks to fully automatic 90 C ContraCon decontamination routine with proven effectiveness e Cultures protected from drying out by unique fast humidity recovery times All HERAcell i incubators are available with optional O2 control Design The iCAN Interactive Control Access Navigator touchscreen improves monitoring and control gives quick access to all the important incubation para
97. benefits of foam and cotton head swabs Foam exterior resists abrasion and most solvents e Cotton core is highly absorbent Model 149 0336 149 0335 Head width mm 9 5 6 4 Head length mm 23 8 19 1 Handle width mm 2 5 2 9 Handle length mm 152 152 Total length mm 176 171 Description Pk Cat No Foam over cotton head 500 149 0336 mall foam over cotton hea Tel 0800 22 33 44 freephone Email uksales uk vwr com 300 10 Fax 01455 55 85 86 Web http uk vwr com Sampling Bags and Swabs VWR Critical Swab Cotton Head Swabs VWRED a ee _ al 149 0331 149 0332 149 0340 149 0341 149 0342 e Highly absorbent e Low linting formulations reduce contamination e Economical Model 149 0342 149 0340 149 0341 149 0331 149 0332 Head width mm 3 2 4 7 4 4 4 8 5 9 Head length mm 14 11 15 15 9 17 4 Handle width mm 1 5 21 5 2 5 2 5 2 5 Handle length mm 75 5 82 78 152 152 Total length mm 89 5 93 93 168 170 Description Pk Cat No Double slim highly absorbent cotton heads packaged in an anti static leaf pack white 2500 149 0342 Double cone shaped highly absorbent cotton heads packaged in an anti static leaf pack white 1250 149 0340 Double highly absorbent cotton heads packaged in an anti static leaf pack white 2500 149 0341 Absorbent cotton tip and wooden shaft autoclavable 1000 149 0331 Lar
98. ble tip e Colour coding Multi and Uno samplers e Ultrapure materials stainless steel PTFE 3 lengths from 55 mm to 150 cm e Excellent finishing robust construction Email uksales uk vwr com Web http uk vwr com 300 2 Sampling Samplers Solid Length Imm depth Description mm mm Chambers Volume ml _ mm Pk Cat No 430 3 14 25 1 312 1111 Multi sampler aluminium 85 710 3 17 25 1 312 1112 150 1355 5 17 25 1 312 1113 Multi sampler stainless 55 430 3 14 25 1 312 1101 steel PTFE 55 430 1 17 25 1 312 1131 Uno sampler stainless steel PTFE 85 710 1 17 25 1 312 1132 Alkiayer sampler 55 430 3 70 25 1 312 1183 alurmini m 85 710 3 130 25 1 312 1185 150 1355 5 260 25 1 312 1184 Jumbo sampler aluminium 350 830 ca 1 atatia 1500 1355 5 1700 50 1 312 1122 Accessories Description Pk Cat No Case for transport 1 312 1141 Tube cleaning brush 25 mm 1 m 1 331 2170 g Ice Borer 300 3 semi solid substances The sharp sawing crown of the very robust hollow borer rapidly cuts into the sample A cylindrical sample cross sectional sample with a diameter of 18 mm and a length of up to 20 cm can be cut out Consists of three parts borer borer head for use in conventional drills and rod for pressing the cylindrical sample out of the borer head Diameter ext 21 mm diameter int 18 mm Stainless steel Ideal both for deep frozen products in the food industry and e
99. chart recorder for on screen viewing of trends virtual instrument display on screen alarm displays and remote set point adjustment For full details of incubator specifications see Series 3 z Temperature range 10 to 50 C volumes 90 to 450 I e Units feature automatic defrost e Easy to use controls with digital display of actual temperature on the standard controller or display of both set point and actual temperature on the programmable controllers e Hermetically sealed refrigeration system and suppressed RF and TV electronics e Full PID heating control to ensure stable internal temperature Inner Shelves supplied Type volume I WxDxH ext mm WxDxH int mm max Pk Cat No 100WNP 90 610x600x810 540x440x490 2 5 1 390 0082 200WNP 227 680x570x1470 550x450x920 3 20 1 390 0086 300WNP 290 680x570x1780 550x450x1 170 3 25 1 390 0090 400WNP 450 680x860x1780 550x700x1 170 3 25 1 390 0097 Internal dimensions shown above are maximum dimensions internal usable space will be less due to space occupied by refrigeration system Please note that with the maximum number of shelves fitted in a cabinet there is only a 20 mm gap between each shelf Cooled Incubators Series 4 Temperature range 10 to 50 C volumes 600 to 1200 Series 4 cooled incubators are temperature controlled cabinets with fan assisted air circulation via a pre mixing chamber They are ideal for studying growth patterns of plants an
100. cientific Temperature range ambient 5 to 70 C volumes 64 to 233 All Heraeus Function Line incubators are designed for unsupervised long term operation These compact units provide maximum possible volumes with the smallest possible footprint and are equipped with all the essentials needed to perform cost effectively in a wide range of laboratory environments With corrosion resistant stainless steel interiors and removable perforated shelves Function Line models are easy to clean and decontaminate e Small footprint but large usable space inside e Configured for unsupervised long term operation e Simple to adjust and easy to clean e Superior temperature homogeneity and fast recovery times e Safe and reliable Cleaning and disinfection These incubators are exceptionally easy to clean and disinfect with rounded edges and corners on all chambers Easily replaceable silicone door seals and quickly dismounted glass doors mean that the usable space can be cleaned thoroughly safely and easily Control Convenient temperature control with preset programme steps up to 70 C The temperature is controlled by precise microprocessor controllers with a large easily readable display A timer with a range from 1 minute to 99 hours for switching the device on and off is integrated in the controller Pre configured temperature time programmes provide further advantages such as fixed heating and cooling ramps delayed heater activation or heat
101. culture surface chemistry improves attachment spreading and growth for many primary cells or cell lines e Optically clear and no special storage required e Convenient peel open medical style packaging Recommended Description working volume ml Culture area cm Pk Cat No BD Primaria Easy Grip dishes 35x10 mm 2 5 3 0 TIT 200 734 0070 BD Primaria standard dishes 60x15 mm 6 0 7 0 21 29 200 734 0071 e BD Primaria standard dishes 100x20 mm 16 0 17 5 58 95 200 734 0072 e BD BioCoat Collagen I Cell Culture Dishes BD Biosciences PS coated with rat tail collagen type I sterile non pyrogenic Collagen found in most tissues and organs is most plentiful in dermis tendon and bone It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture In vitro use of collagen can exert effects on the adherence morphology growth migration and differentiation of a variety of cell types e Manufactured in a highly controlled environment and rigorously tested to assure product consistency and performance Stable at room temperature when stored in dry conditions Description Pk Cat No i 20 734 0141 Culture dish 35 mm 100 734 0281 20 734 0109 100 734 0275 10 734 0136 E O e S A Culture dish 60 mm Culture dish 100 mm Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 22
102. d 1000 ml 38 430 12 215 2209 g Amber round 500 ml 38 430 12 215 2208 g Caps PP Description Thread Pk Cat No Standard cap blue 38 430 48 215 2227 Standard cap blue 38 430 96 215 2207 e Septum cap blue 38 430 96 215 2212 Vented cap with PTFE membrane sterile 38 430 72 215 2225 e Vented cap with PTFE membrane sterile 38 430 48 215 2226 Media Bottles Square Sterile Nalgene An inexpensive alternative to glass bottles for storage and transport of media these heavy walled square media bottles are transparent and graduated PETG bottle with HDPE screw closure sem e Leak proof break resistant and durable e Reduced permeability to carbon dioxide and oxygen e Bottles and closures are radiation sterilised and non pyrogenic to eliminate costly washing depyrogenation and autoclaving steps e Heat shrink band around closure and neck provides tamper evident seal e 2L size has moulded in hand grips and a 53 mm white closure 392 37 VWR 3 j www vwr com Cell Culture Cell Culture Bottles and Tubes Capacity ml Cap size Pk Cat No 30 20 mm 96 215 6700 g 60 24 mm 96 391 7122 125 38 430 48 391 7123 250 38 430 48 391 7124 500 38 430 24 391 7125 1000 38 430 24 391 7126 g 2000 53B 12 391 7127 e Culture Media Bottles Square Sterile Nalgene PETG An inexpensive alternative to glass bottles for storage and transport of srete media Available with or without a natural HD
103. d stackable and compatible with most standard microplate readers and washers e Standard 96 well plates have coloured alphanumeric code identifying surface type cell culture treated orange immuno treated blue non treated grey e White plates provide maximum reflection and minimum crosstalk during luminescence measurements black plates provide minimum back light scatter during fluorescence analyses Sterile plates are supplied individually wrapped with lid BRANDplates are non cytotoxic according to ISO 10993 5 free of endotoxins lt 0 01 EU ml and free of DNA DNase and RNase Sterile products comply with ISO 11137 and AAMI guidelines cellGrade The standard surface for cultivation of adherent cell lines Chemical groups on the surface of the plate bind to serum compounds stimulating the growth of immobilised cells Description Colour Culture area cm Well volume Pk Cat No Standard plate with U bottom transparent 0 32 330 ul 50 735 2097 Standard plate with V bottom transparent 0 33 360 ul 50 735 2098 Standard plate with F bottom transparent 0 32 350 ul 50 735 2099 Standard plate with F bottom white 0 32 350 ul 50 735 2100 Standard plate with F bottom black 0 32 350 ul 50 735 2101 Standard plate with C bottom transparent 0 25 350 ul 50 735 2102 Transparent base with F bottom white 0 31 330 ul 50 735 2103 Transparent base with F bottom black 0 31 330 ul 50 735 2104 cellGrade plus This surf
104. d insects BOD tests seed germination tissue culture enzyme testing and fruit fly culture e Units feature automatic defrost e Easy to use controls with digital display of actual temperature e Hermetically sealed refrigeration system and suppressed RF and TV electronics e Full PID heating control to ensure stable internal temperature Design The units have a stainless steel exterior stainless steel lined interior with polyurethane foam insulation and feature a door lock and magnetic door gasket Shelves Supplied with white plastic coated wire shelves and self evaporating condensate drip tray Cleaning Corrosion resistant inner chamber is easy to clean disinfection procedures are detailed in the user manual Safety Features include variable over temperature alarm safety cutout preset at 70 C and refrigeration isolation switch Controller Controls are recessed in the control panel to avoid accidental alteration Full PID heating control indicators for mains and high temperature safety cut out Connections 230 V 50 60 Hz RS 232 interface Technical data Average temperature variation 0 5 C and fluctuation of 0 1 C with an average load Heating time 20 to 45 C in 30 min cooling time 20 to 0 C in 50 min subject to load and ambient temperature Calibrations Optional conformance certificate and on site UKAS Accreditation of laboratory equipment are available Please enquire for details Tel 0800 22 33 44 f
105. d plate with F bottom low volume black 30 0 07 50 735 2110 Transparent base with F bottom white 120 0 13 50 735 2111 Transparent base with F bottom black 120 0 13 50 735 2112 cellGrade plus This surface has a protein like structure Cultivation of cells in serum reduced media is possible cellGrade plus surface is suitable for the cultivation of more fastidious cell lines Well volume Culture area Description Colour ul cm Pk Cat No Standard plate with F bottom transparent 100 0 12 50 735 2121 Transparent base with F bottom white 120 0 13 50 735 2122 Transparent base with F bottom black 120 0 13 50 735 2123 Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com Web http uk vwr com 392 12 Cell Culture Cell Culture Plates and Dishes cellGrade premium This surface is a Poly D Lysine equivalent With the optimised surface characteristics the most fastidious cell lines can be cultivated Cells show growth comparable to Poly D Lysine surfaces Well volume Culture area Description Colour ul cm Pk Cat No Standard plate with F bottom transparent 100 0 12 50 735 2129 Transparent base with F bottom white 120 0 13 50 735 2130 Transparent base with F bottom black 120 0 13 50 735 2131 inertGrade Surface inhibits cell binding Especially suited for the cultivation of cells when adhesion is not desired for example when working with cell suspe
106. e HTLV III virus They are colour coded for easy specimen identification and to help guard against cross contamination The top of each cap can also be numbered or labelled with indelible ink Self locking cap holds firmly in any position and keeps contents secure while in transit Liquid tight seal withstands centrifugation agitation refrigeration and freezing e Colour coded self locking test tube caps prevent spillage or loss of samples e Fit all varieties of blood collecting and disposable culture tubes Ordering Information caps are packaged in bags of 1000 Description Colour Pk Cat No transparent 1000 548 0720 red 1000 548 0733 lilac 1000 548 0731 Safe T Flex caps for 10 mm blood collecting and culture tubes green 1000 548 0735 blue 1000 548 0728 grey 1000 548 0737 yellow 1000 548 0734 transparent 1000 548 0711 red 1000 548 0722 lilac 1000 548 0716 Safe T Flex caps for 12 mm culture tubes and 13 mm blood collecting green 1000 548 0713 lubes blue 1000 548 0718 grey 1000 548 0725 yellow 1000 548 0727 transparent 1000 548 0710 red 1000 548 0723 lilac 1000 548 0719 Safe T Flex caps for 13 mm culture tubes green 1000 548 0724 blue 1000 548 0714 grey 1000 548 0732 yellow 1000 548 0730 392 39 VWR 3 j www vwr com Cell Culture Cell Culture Bottles and Tubes Description Colour Pk Cat No transparent 1000 548 0712 red 1000
107. e Sterilised by gamma irradiation e Packaged in peel open medical style packaging e Multi unit bags have reseal tabs Description Pk Cat No Round dish 60 mm volume 23 0 ml 500 734 0068 1 Well round dish 60 mm well volume 2 5 ml 500 734 0398 4 Well plate well volume 1 8 ml individually wrapped 100 734 0069 Instant value with Wwww vwr com Please contact your local sales office for more information 392 23 VWR oy www vwr com Cell Culture Cell Culture Plates and Dishes Culture Dishes for In Vitro Fertilisation Nunc PS sterile flat bottom with lid 3 s These fully certified dishes are specifically intended for in vitro fertilisation e j IVF use i 3 e Choice of Nunclon A treated or non treated surfaces m p e Full batch control of all components in the final product giving full a traceability e Unique certification based on a 1 cell stage mouse embryo toxicity test confirms sterility SAL 106 non pyrogenicity and that the material has passed USP class VI requirements Description Pk Cat No Nunclon A treated 4 well dish 66x66 mm culture area 1 9 cm well 120 734 1175 Untreated dish 60x15 mm culture area 21 5 cm 400 391 0108 Untreated dish 40x12 mm culture area 8 8 cm 500 391 0109 IVF Culture Dishes Costar Corning Optically clear virgin PS IVF Culture Dishes with 20 mm centre well Inner well holds 3 ml of medium while the outer we
108. e peel pouch SRK Foam Spatulas Small spatula attached to cap Large foam spatula 22 cm long 612 2678 Description Packed Pk Cat No SRK Rinse Solution 10 ml In plastic peel pouch 50 612 2679 SRK without medium In plastic peel pouch 50 612 2680 SRK large without medium In plastic peel pouch 120 612 2678 SRK 471 1 Tube 8 plain swabs in tube blue applicator in minigrip pack Description Packed Pk Cat No SRK Rinse Solution rayon 10 ml 6x25 minigrips per case 150 710 0479 Swabs Steriswabs 300 15 Blue plastic shafted swabs with 45 mm breakpoint dry or pre moistened with sterile diluent designed for effective monitoring of sterility and contamination in clinically clean or sterile work areas e Available in labelled tube or peel pouches e Triple wrapped and radiation sterilised Description Pk Cat No Steriswab blue plastic shaft labelled tube 250 720 0142 e Available with various applicators and packaging wood plastic straight or twisted wire applicators e Suitable for all requirements Description Pk Cat No Wood stick peel pouch charcoal 1250 720 2200 Wood stick peel pouch 800 720 2203 Fine tip plastic stick labelled tube 100 720 0122 100 720 0124 800 720 0123 100 720 0126 800 720 0125 Plastic stick labelled tube Wood stick labelled tube ENT straight wire labelled tube 100 720 0133 Pernasal tw
109. e transparent 5 734 0116 BD BioCoat Laminin Cell Culture Plates BD Biosciences PS coated with mouse laminin sterile non pyrogenic Laminin a major structural component of basement membranes has many varied functions that are mediated by binding to various components of the basement membrane for example collagen IV and to cell surface receptors Laminin coated cell culture plates have a number of applications including the promotion of cell adhesion proliferation and differentiation of a variety of cell types particularly neurons epithelial cells myocytes and myoblasts Description Colour Pk Cat No 6 Well plate transparent 5 734 0112 24 Well plate transparent 5 734 0118 96 Well plate transparent 5 734 0117 Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com Web http uk vwr com 392 18 Cell Culture Cell Culture Plates and Dishes 96 Well Cell Culture Plates Poly D lysine Coated Corning PS flat bottom without lid _ Enhanced cell attachment and binding especially in assays for difficult to attach cells e Helps cells stay attached during washing steps e Aseptically manufactured e Black plates are designed for lower background in fluorescent assays and reduce crosstalk white plates are designed for luminescent assays Description Pk Cat No Standard clear plate poly D lysine coated 100 734 1618 White plate with clear bottom poly D lysine co
110. e vent 90 14 2 825 391 1506 Without vents 90 16 2 825 391 1507 g With one vent 90 16 2 700 391 1509 Petri Dishes Sterilin grade of crystal polystyrene and are suitable for all bacteriological applications 90 mm dishes are manufactured and packed in aseptic conditions The range includes 50 mm and 60 mm dishes ideal for use in vacuum tests using membrane filters for microbiological analysis of liquids large dishes for antibiotic assays and sensitivity testing compartmented dishes for culturing on two or three different media using the same plate The Repli dish has 25 compartments each measuring 1 8 cm square capacity 5 ml and has many applications The 55 mm Contact plate is used for surface sampling in the food industry hospitals and other areas where contamination levels are of importance It has a surface area of 24 sq cm and there is a numbered grid on the base to facilitate colony counting These plates should be poured on a cold surface to enhance the meniscus High quality single use petri dishes are moulded in a specially developed srete e Suitable for use with automatic plate pouring machines e Aseptically manufactured under clean room conditions class 7 15014644 to exclude microbiological contamination e Ideal for use with different media or when savings in media incubator space are required with compartments Height Version mm mm Pk Cat No Triple vent 35 11 800 3
111. e with 250 um well 5 734 2207 Tel 0800 22 33 44 freephone Email uksales uk vwr com Fax 01455 55 85 86 Web http uk vwr com 392 28 Cell Culture Cell Culture Chamber Slides LiveCell Array Cell Surface Marker Kits 5 slides plus reagents Description Pk Cat No Slide with 15 um well 5 734 2208 Slide with 20 um well 5 734 2209 Slide with 100 um well 5 734 2210 Slide with 250 um well 5 734 2211 BD Falcon Cell Culture Inserts BD Biosciences PET membranes in PET housing sterile BD Falcon Cell Culture Inserts are track etched low protein binding PET membranes with a smooth surface and defined cylindrical pores that transverse the membrane Available in a wide range of configurations 6 12 and 24 well and a broad selection of pore sizes 0 4 1 0 3 0 and 8 0 um Larger pore size membranes are most suitable for investigating chemotaxis invasion and migration transparent membranes for visualisation of cells by light microscopy and high pore density membranes for maximum diffusion when studying transport secretion or drug uptake e Non tissue culture treated insert housing prevents growth of cells on the inert walls e Hanging design facilitates pipetting and allows for co culture e Suitable for use with BD Falcon Cell Culture Insert Companion Plates e Supplied in individual blister packs Transparent PET membrane 0 4 um pore size 1 6x10 pores cm Description
112. ecovery Solution BD Biosciences BD Cell Recovery Solution allows for the recovery of cells cultured on BD Matrigel Basement Membrane Matrix for subsequent biochemical analysis BD Cell Recovery Solution depolymerises BD Matrigel Matrix gels without enzymatic digests and lengthy incubation periods at high temperatures Cells are released without damage thereby avoiding biochemical changes during incubation and digestion of extracellular portions of cell surface receptors and adhesion molecules Formulation Non enzymatic proprietary solution Tested for ability to depolymerise a 1 mm thick layer of gelled BD Matrigel Matrix after one hour at 2 8 Quality control C Filtered and tested and found negative for bacteria fungi and mycoplasma Storage and stability Stable for at least three months at 2 8 C Description Pk Cat No BD cell recovery solution 100ml 734 0107 392 63 VWR 3 j www vwr com Cell Culture Cell Culture Media and Supplements Ampicillin Sodium Salt AppliChem Ampicillin is a half synthetic penicillin amp lactam antibiotic known to interfere with the wall peptide crosslinking in growing bacteria It inhibits the enzyme activity of transpeptidase carboxypeptidase and endopeptidase Its action is bactericidal and the pH optimum for its action ranges from pH5 5 6 Ampicillin sodium salt is readily water soluble and stock solutions are prepared at concentrations of 50 mg ml stored at
113. ed with 2 perforated stainless steel shelves choice of 3 to 5 shelf positions depending on size of incubator Cleaning and disinfection Corrosion resistant stainless steel chamber and interior fixtures easy to clean due to shape and material Control Multifuctional microprocessor PERFECT P D controller with 8 digit alphanumeric digital display digital 7 day programme timer with real time clock precise minute setting for one set value or start of ramp operation Safety independent digital adjustable electronic microprocessor overtemperature controller TWW protection Class 3 3 Connections USB interface Technical data Temperature deviation lt 0 1 C in real time Calibration Calibration and validation to assist with GLP compliance are possible please enquire Accessories Wide range of options including interfaces Celsius software and chip cards Please ask for details Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No IPP 200 32 550x490x600 400x250x320 1 390 0270 IPP 300 39 630x490x600 480x250x320 1 390 0238 IPP 400 53 550x550x680 400x330x400 1 390 0271 IPP 500 108 710x620x760 560x400x480 1 390 0272 Stocked articles in our UK warehouse are marked with a green dot Articles stocked in our European Warehouses are marked with an amber dot Please contact your local sales office for more information 390 10 VWR 25 in www vwr com Cell Culture Incubators Low
114. enic Tel 0800 22 33 44 freephone Email uksales uk vwr com Fax 01455 55 85 86 Web http uk vwr com e White plates are designed for luminescent assays e Treated for optimal cell attachment e Black plates are designed to lower background in fluorescent assays and reduce 392 14 Cell Culture Cell Culture Plates and Dishes Description Pk Cat No Solid white plate TC treated without lid 100 734 1549 Black plate with clear bottom TC treated individually wrapped 48 734 1609 White plate with clear bottom TC treated individually wrapped 48 734 1610 White plate with clear bottom TC treated 100 734 1660 Black plate with clear bottom TC treated 100 734 1661 Solid black plate TC treated 100 734 1664 Solid white plate TC treated 100 734 1665 384 Well Clear Bottom Cell Culture Plates Corning PS flat bottom with lid Black plates are designed to lower background in fluorescent assays and reduce cross talk White plates are designed for luminescent assays e Treated for optimal cell attachment e Sterilised by gamma radiation e Certified non pyrogenic Description Sterile Pk Cat No Standard clear plate poly D lysine coated 100 734 1615 White plate with clear bottom poly D lysine coated 100 734 1616 Black plate with clear bottom poly D lysine coated 100 734 1617 Black optical imaging plate with clear bottom TC treated 100 734 1200 Optical Bottom
115. er time e g ramp changes where a gradual rate of change can be set or step changes which are instantaneous These can be separated by soak periods during which the process is held at a constant value Each individual time interval of the program or segment together with its associated moving set point value can be stored as a unique program Via use of event outputs control of interior illumination and or alarm function is also available At the end of a sequence a program can be arranged to repeat or loop either a specified number of cycles or continuously For safety reasons three modes of recovery from power failure are available These either automatically re start the program from the beginning continue it from where it stopped or hold it waiting for a user re start Finally chart recorder data logging and configuration software for windows is available as optional extras This software is designed to operate to program all functions of the controller and to data log the recorded process signals including chart recorder for on screen viewing of trends virtual instrument display on screen alarm displays and remote set point adjustment For full details of incubator specifications see Series 4 ra Temperature range 10 to 50 C volumes 600 to 1200 e Units feature automatic defrost e Easy to use controls with digital display of both set point and actual temperature e Hermetically sealed refrigeration system and suppressed R
116. es attachment spreading and growth for many primary cells or cell lines e Vented caps incorporate a 0 2 um hydrophobic membrane e Optically clear and no special storage required e Convenient peel open medical style packaging Growth area cm Capacity ml Neck Cap Pk Cat No 25 50 canted vented 100 734 0073 canted plug seal 200 734 0075 straight vente l e 75 250 straight plug seal 100 734 0076 Note culture area and capacity are nominal 392 5 VWR 3 j www vwr com Cell Culture Cell Culture Flasks BD BioCoat Collagen I Cell Culture Flasks BD Biosciences PS coated with rat tail collagen type I sterile non pyrogenic The uniform application of collagen type to the surface of tissue culture vessels has been found to improve cell attachment and increase proliferation rates for a variety of normal or transformed mammalian cell types Growth area cm Capacity ml Neck Cap Pk Cat No canted vented cap 10 734 0158 25 70 canted vented cap 50 734 0289 canted plug seal 10 734 0187 canted plug seal 50 734 0309 canted vented cap 5 734 0159 canted vented cap 50 734 0290 75 250 canted plug seal 5 734 0147 canted plug seal 50 734 0283 150 600 straight vented cap 40 734 0291 straight plug seal 40 734 0318 straight vented cap 40 734 0292 traight lug seal 5 734 01 175 750 S raig plug sea 34 0155 straight plug seal 40 734 0287 straight vented cap 5 734
117. escription Pk Cat No onical tube 50 mi with flip top cap BD Falcon Cell Culture Tubes BD Biosciences Cell Culture Tubes are tissue culture treated for cell attachment and spreading e Compatible with most tube rolling equipment e Convenient cross hatched index mark assists in positioning tubes e White screw caps distinguish the tissue culture treated tubes from general purpose tubes and provide capability for both open and closed incubation Description Pk Cat No Cell culture tubes 16x125 mm 500 734 0015 Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 40 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Bottles and Tubes BD Falcon Round Bottomed Test Tubes BD Biosciences For reliable containment of laboratory fluid samples PP tubes For applications requiring greater thermal and chemical stability PS tubes For procedures requiring high optical clarity e Widely used and referenced in laboratory protocols e Specialised tube for flow cytometry applications e Cell strainer cap tube has a 35um cell strainer mesh incorporated into cap e Dual position snap caps with heavier gauge walls provide a secure positive seal Description Pk Cat No PS 12x75 mm 5ml snap cap sterile 1 pk 500 734 0436 PS 12x75 mm 5ml snap cap sterile 25 pk 500 734 0445 PS 12x75 mm 5ml snap cap sterile
118. for storing liquids e Strong transparent and waterproof e Heavy duty zipper seals contents Thickness 0 1 mm Description Pk Cat No 102x152 mm 50 216 8121 127x203 mm 50 216 8122 e 152x330 mm 50 216 8123 229x330 mm 50 216 8124 g 229x457 mm 50 216 8125 e e Additional pocket 170 mm for documents Thickness 50 um Description Pk Cat No Bags 160x220 mm 1000 129 2014 e VWRED www vwr com Sampling Bags and Swabs PE Self seal with white panels 200 gauge Description Pk Cat No Bag 57x57 mm 1000 129 0916 Bag 89x114 mm 1000 129 0917 Bag 102x140 mm 1000 129 0918 Bag 152x229 mm 1000 129 0919 Specimen Bags Mini grip Biohazard PE Specimen bags with front compartment for specimen 150x140 mm and back pocket for report e Thumb and fingers press seal across upper section e Plain for own labelling or printed with biohazard symbol WxH mm Pk Cat No 150x140 500 129 0412 150x140 500 129 0342 Storage Bags Clear PE plain Heavy duty storage bags 500 gauge Suitable for heat sealing WxH mm Pk Cat No 150x200 100 300 0045 255x355 50 300 0046 355x510 50 300 0047 e 455x760 50 300 0048 e VWR Critical Swab Foam over cotton Head Swabs VWRES _ _ _ _ M 149 0335 149 0336 Disposable foam over cotton tip swab features 100 polyurethane foam with 100 ppi and a wooden shaft Combine the
119. frigerant R 134a Suitable for stability tests according to ICH guideline Q1A e Electronically controlled APT line preheating chamber and refrigeration system Design Inner glass door with smooth inner face and seals access port with 30 mm silicone plug on the left includes 2 stainless steel racks and Binder test certificate Control MCS controller with colour LCD screen with 25 storable programs each with 100 sections for max 500 program segments Integrated electronic chart recorder variety of options for the graphic display of process parameters and a real time clock Safety Adjustable temperature safety device Class 3 1 DIN 12880 with visual and audible alarm Complete safety connection kit for water supply and drainage Humidity Microprocessor controlled humidification and dehumidification system with a humidity range of 10 80 RH humidity accuracy levels of 1 5 RH Connections 230 V Ethernet or RS422 interface for communication software Calibration Calibration and validation possible please enquire KBF series Shelves Inner volume supplied Type 1 WxDxH ext mm WxDxH int mm max Pk Cat No KBF 115 115 834x645x1250 600x400x480 2 5 1 390 0230 KBF 240 240 905x765x1458 650x470x785 2 9 1 390 0313 KBF 720 720 1234x865x1983 1000x600x1200 2 14 1 390 0232 KBF ICH series Inner volume Shelves supplied Type I WxDxH ext mm WxDxH int mm max Pk Cat No KBF ICH 240 240 905x765x14
120. fungi Storage and stability Store at 20 C in original packaging Stable for at least 3 months at 20 C BD BioCoat Matrigel Invasion Chambers Description Pk Cat No 8 0 um Inserts in four 6 well plates 24 734 1048 8 0 um Inserts in two 24 well plates 24 734 1047 BD BioCoat Growth Factor Reduced GFR Matrigel Invasion Chambers Description Pk Cat No 8 0 um Inserts in two 24 well plates 24 734 1049 BD BioCoat 24 Multiwell Tumour Invasion System Description Pk Cat No 8 0 um Multiwell insert plate in one 24 well plate 1 734 1024 8 0 um Multiwell insert plate in five 24 well plates 5 734 1025 BD BioCoat 96 Multiwell Tumour Invasion System Description Pk Cat No 8 0 um Multiwell insert plate in one 96 well plate 1 392 2500 8 0 um Multiwell insert plate in five 96 well plates 5 392 2501 BD BioCoat Angiogenesis System BD Biosciences Angiogenesis is the development of new blood vessels from pre existing ones This process is essential for normal growth and homeostasis However angiogenesis becomes altered during certain disease states which results in excessive or insufficient blood vessel formation Diseases such as cancer diabetic retinopathy and rheumatoid arthritis are characterised by excessive angiogenesis The BD BioCoat Angiogenesis Systems facilitate investigation of compound effects on endothelial cell invasion migration and tubulogenesis The a
121. g volume ml Culture area cm Pk Cat No Inserts for 6 well multidishes 25 mm 1 75 4 2 48 734 2022 rt pack well multidish 4 Inserts pre packed in 6 well multidishes 23x3 i75 A j4 734 2237 mm Inserts for 24 well multidishes 10 mm 0 5 0 5 48 734 2021 rt pack well multidish Inserts pre packed in 6 well multidishes 20x25 15 3 14 j 734 2231 mm rt packed in 12 well multidishes 12x1 ae s pre packed in 12 well multidishes 12x18 1 1 1 13 48 734 2234 rt pack 24 well multidish 1 Inserts pre packed in 24 well multidishes 8x13 05 0 47 48 734 2229 mm Anopore membrane 0 02 um pore size 10 pores cm Recommended Description working volume ml Culture area cm Pk Cat No Inserts for 6 well multidishes 25 mm 1 75 4 2 48 734 2169 Inserts for 24 well multidishes 10 mm 0 5 0 5 48 734 2077 Anopore membrane 0 2 um pore size 10 pores cm Recommended Description working volume ml Culture area cm Pk Cat No Inserts for 6 well multidishes 25 mm 1 75 4 2 48 734 2014 e Inserts for 24 well multidishes 10 mm 0 5 0 5 48 734 2013 Inserts for 8 well strip of MicroWell plate 0 06 0 08 15 732 2600 g All Nunc Cell Culture Inserts Recommended working volume ml in addition to normal well working volume VWR Collection Please contact your local VWR sales office or go to wwwawrcam for the latest news and special offers 392 33 VWRe F www vwr com Cell Culture Cell Culture Bottles and Tubes Drosop
122. ge absorbent cotton tip and wooden shaft autoclavable 500 149 0332 VWR Critical Swab Foam Head Swabs VWRED 149 0264 149 0265 149 0266 ooo EE a 149 0267 149 0268 149 0269 149 0270 149 0271 149 0272 149 0333 149 0334 149 0337 D a _ E 149 0338 149 0339 149 0343 The VWR Critical Swab line includes foam head swabs cotton head swabs and foam over cotton head swabs suitable for a wide variety of applications Swabs feature 100 ppi open or closed cell polyurethane foam with 100 virgin polypropylene handle Swab heads are thermally bonded to the handle without using adhesive Some have a blue glass filled polypropylene shaft for extra rigidity specially for use in critical environments e Ideal for use in controlled environments e Withstand most widely used solvents e Nonabrasive to protect delicate components 300 11 VWR CF Wwww vwr com Sampling Bags and Swabs Model Head width mm Head length mm Handle width mm Handle length mm Total length mm 149 0343 1 8 14 6 2 4 161 173 149 0272 3 2 20 2 2 59 7 79 7 149 0271 3 4 20 2 5 50 70 149 0268 3 4 10 5 2 4 57 5 68 149 0270 3 5 10 3 0 79 89 149 0269 3 6 12 3 0 59 71 149 0338 3 6 25 4 2 5 82 8 108 149 0337 3 9 17 5 2 5 83 100 149 0333 4 8 17 4 2 5 152 170 149 0265 4 8 12 4 3 93 105 149 0334 4 8 15 1 3 1 152 167 149 0267 6 2 17 3 2 146 163 149 0266 7
123. gene PP white with silicone seal For use and insertion of 7 14 mm diameter probes into Nalgene 1 I and 12 fermenters Seal separates environment from inside of vessel and prevents contamination when using a probe e Thermal resistance from 135 to 135 C e For Nalgene 1 and 12 fermenters Description Pk Cat No Probe adapter closures 2 734 5053 Culture Vessels with Ports with or without BioTech Mixer Nalgene PC culture vessel with white PP closures Lightweight and break resistant Features 4 shoulder access ports Graduated in 0 5 increments from 3 to 12 Made from food grade resins that meet the requirements of USP Class VI Non cytotoxic e Thermal resistance 135 to 135 C autoclavable e Shatter proof with high mechanical strength Brim capacity 15 Closure diameter top 100 mm Closure size side 38 430 Overall height 429 mm Outer diameter 289 mm Culture vessels with BioTech mixer The Culture Vessel System with BioTech Mixer consists of a 12 culture vessel with ports as described above 1 8 HP overhead drive BioTech mixer and a lower assembly comprising 340 mm shaft with 100 mm long axial flow glass filled PP impeller and 63 5 mm wide PP baffle The BioTech mixer provides variable speed programmable speed duration control clockwise and counter clockwise rotation and is specifically designed for maximum efficiency with system components Vessel and lower assembly are a
124. h collagen Cell culture on permeable membranes permits diffusion of media components to both apical and basolateral cell surfaces similar to the in vivo process Membranes with extracellular matrix ECM further improve in vitro cell culture systems by providing cells with a vital component of their microenvironment in vivo Typical applications for BD BioCoat Cell Culture Inserts include promotion of epithelial cell polarity differentiation of a variety of cell types transport and permeability studies transendothelial migration tumour cell invasion assays in vitro toxicology and co culture studies Delivery Information Supplied packaged ready to use in BD Falcon Companion Cell Culture Plates Pack quantity refers to the total number of cell culture inserts supplied For further details about the full range of BD BioCoat Cell Culture Inserts please contact your local VWR representative BD BioCoat collagen cell culture inserts Description Pk Cat No Four 6 well plates 0 4 um 24 734 0131 Four 6 well plates 1 0 um 24 734 0222 Four 6 well plates 3 0 um 24 734 0195 BD BioCoat collagen IV cell culture inserts Description Pk Cat No Two 24 well plates 1 0 um 24 734 0227 BD Falcon Cell Culture Insert Companion Plates BD Biosciences PS tissue culture treated sterile non pyrogenic with lid seme Specifically designed for use with BD Falcon or BD BioCoat Cell Culture Inserts so that evapora
125. helves Accessories and factory fitted options such as USB and printer interfaces access ports and documentation are available on request please enquire for details Description Pk Cat No HCP 108 1 466 5053 HCP 153 1 466 5054 HCP 246 1 466 5055 Tel 0800 22 33 44 freephone Email uksales uk vwr com 390 17 Fax 01455 55 85 86 Web http uk vwr com Cell Culture CO Incubators CO Incubators with Hot Air Sterilisation CB Series Binder Temperature range ambient 7 to 60 C volume 53 150 or 210 I COz incubators with advanced technology so all growth parameters are maintained in constant equilibrium with the shortest reaction times and growth processes are reproducible at any time The CB series enable cell cultivation in the most up to date working conditions and reliable tissue production in compliance with all international directives and regulations The precision and outstanding dynamics of the new screen controllers are excellent All values are displayed in real time The exceptionally safe decontamination concept guarantees complete sterility The CB series is ideal for all sensitive incubation applications Electronic self diagnostic system for errors with optical and acoustic alarm as well as potential free relay contact for central monitoring e Electronically controlled APT line preheating chamber technology MCS controller for temperature and CO2 concentration O e Permadry
126. hila Stock Bottles VWR Ee F s PP round or square bottom or PE square bottom bottles w e Thin wall design for excellent visibility e Pliable top for easy removal of paper lid e Moulded in food level graduation marks Bottle 734 1259 features a wider edge for a more secure paper cap fit Bottle 734 1250 has a moulded indent near the bottom which helps to secure media PP bottles are autoclavable PE bottles are not Capacity Description ml Pk Cat No PP square bottom 170 500 734 1260 PE square bottom 170 500 734 1261 PP square bottom with wider edge 170 500 734 1259 PP round bottom 170 500 734 1249 e PP round bottom with moulded indent 225 250 734 1250 VWRE Available in three materials and two packaging formats Ideal for growing yr drosophila or other insects Straight wall shell design allows for easy racking filling and plugging e PP vials are virtually unbreakable economically priced and are autoclavable e PS vials are clear and provide a safe and economical alternative to glass vials e K Resin co polymer vials offer glasslike clarity are scratch resistant and are ideal for microscopic examination they are more flexible than PS vials which makes them excellent for freezing and shipping specimens PS and K Resin vials are not autoclavable Sizes Narrow 25 x 95 mm wide 28 5 x 95 mm short 24 x 75 mm Bulk packed vials Bulk packed vials are layered and are open end orientated for conven
127. hing component for wear free noiseless heater switching Actual and set point temperature are displayed digitally Kelvitron microprocessor controller RS232 computer interface 24 hour timer and temperature monitor class 2 DIN 12880 part 1 Safety Electrical equipment in line with VDE 0700 All sizes are GS and SEV tested Protection class IP 20 Constructed according to DIN 12880 Adjustable overheating monitor protection class 3 1 as standard Connections 230 V 50 60 Hz 0 26 1 20 kW according to model in addition to the RS232 digital interface there is the option of temperature documentation via analogue signals Models with natural convection Shelves supplied Type Inner volume I _ WxDxH ext mm WxDxH int mm max Pk Cat No B 6200 196 895x715x840 554x550x644 2 18 1 390 1140 B 6030 30 552x535x576 352x370x231 1 4 1 390 1110 B 6060 60 744x535x576 403x370x380 2 9 1 390 1120 B 6120 107 895x535x720 554x370x524 2 14 1 390 1130 B 6420 409 744x715x1813 544x550x1366 2 39 1 390 1150 B 6760 751 1200x715x1813 1000x550x1366 2 39 1 390 1160 Models with forced convection Shelves supplied Type Inner volume I _ WxDxH ext mm WxDxH int mm max Pk Cat No UB 6420 409 744x715x1813 544x522x1319 2 39 1 390 0040 UB 6760 751 1200x715x1813 1000x522x1319 2 39 1 390 0041 390 6 VWR EZ F www vwr com Cell Culture Incubators General Purpose Incubators Genlab Temperature range ambient 5 to 70
128. id attachment e Sterilised by gamma irradiation e Certified non pyrogenic e CelIBIND Surface increases wetability for more even and consistent cell seme Cell Culture Cell Culture Plates and Dishes e Ultra Low Attachment plates feature a covalently bound hydrogel layer that minimises cell attachment protein absorption and cellular activation Description Pk Cat No Standard plate flat bottom CellBIND surface 50 734 4058 Standard plate flat bottom Ultra Low Attachment individually wrapped 24 734 1585 Standard plate flat bottom TC treated with low evaporation lid 50 734 1789 Standard plate flat bottom TC treated with low evaporation lid individually wrapped 50 734 1793 Standard plate flat bottom TC treated individually wrapped 50 734 1794 Standard plate flat bottom TC treated individually wrapped 100 734 1796 Round bottom plate TC treated individually wrapped 50 734 1797 Standard plate flat bottom TC treated 50 734 1799 Round bottom plate Ultra Low Attachment individually wrapped 24 444 1020 96 Well Plates Untreated Corning PP plate with PS lid Sterilised by gamma radiation e Certified non pyrogenic Description Pk Cat No 96 Well plate with round bottom individually wrapped 50 732 5541 96 Well Cell Culture Plates for Fluorescent and Luminescent Applications Corning PS cross talk e Sterilised by gamma irradiation e Certified non pyrog
129. ient handling Description Pk Cat No PP vials narrow 500 734 1244 vials wide g PS vials narrow 500 734 1254 e PS vials wide 500 734 1255 e PS vials narrow shot BOO 734 1257 K Resin vials narrow 500 734 1246 K Resin vials wide 500 734 1248 Tray packed vials Shrink wrapped in a 10x10 row configuration with dividers between vials Description Pk Cat No PP vials narrow 500 734 1253 PP vials wide 500 734 1251 PS vials narrow 500 734 1243 PS vials wide 500 734 1256 PS vials narrow short 500 734 1258 K Resin vials narrow 500 734 1245 K Resin vials wide 500 734 1247 Vial and dividers Cardboard trays with cell dividers 254x254 mm row configuration for narrow or wide diameter shell vials Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 34 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Bottles and Tubes Description Pk Cat No Trays and dividers for narrow vials 50 734 1237 Trays and dividers for wide vials 50 734 1240 Dividers only for narrow vials 50 734 1238 Trays only for narrow vials 50 734 1239 Dividers only for wide vials 50 734 1241 Trays only for wide vials 50 734 1242 BD Falcon Sterile Containers BD Biosciences PP sterile with PE lid Convenient single use containers for collection transport and storage of a wide variety of specimens e Moulded in graduations for easy measurement
130. ies are responsible for most of the contamination in cell culture At the concentrations recommended for use 1 ug ml no cytotoxic effects have been found and the treatment is easy to perform The pH value of Myco 3 is acidic pH 4 5 When added to medium the pH change is negligible Pk Cat No 10 ml A5240 0010 20 mi A5240 0020 T00 ml A5240 0100 The VWR e newsletter Register now at http eu vwr com e newsletter 392 65 VWR 3 j www vwr com
131. igid or flexible the loops have round edges for gentle treatment of the agar e Gamma sterilised seme e Colour coded e Hexagonal shaft for easy handling Delivery Information Delivered with a certification of calibration in each box Description Capacity ul Colour Packed Pk Cat No 1 dark green bags of 10 500 612 9351 ae ark green ags o Loops rigid 70 dark blue bags of 10 500 612 9353 z 10 dark blue bags of 20 1000 612 9354 ight green ags o E 1 light green bags of 20 1000 612 9356 Loops flexible 10 light blue bags of 10 500 612 9357 10 light blue bags of 20 1000 612 9358 e inoculating Needles and Loops Nunc PS sterile Disposable problem free inoculation with smooth loop surface e Meet semi quantitative standard for handling samples e Surface treated for enhanced droplet adhesion e Non toxic Description Capacity ul Colour Packed Pk Cat No 1 transparent 12 2400 710 2508 Loops 1 transparent 50 4000 734 2138 e 10 blue 12 2400 710 2509 10 blue 50 4000 734 2137 yellow 12 2400 734 2140 Needles yellow 50 4000 734 2139 Inoculating Loops Microstreaker Inoculation loop of nickel chromium wire of not more than 60 mm length conforming to Howie recommendations e Colour coded handle Description Capacity ul Pk Cat No Large 5 05 mm ID red handle 10 5 300 0501 e Medium 2 91 mm ID green handle 3
132. ing to a pre selected temperature and selection of a temperature maintenance time These functions can be selected individually or in any combination A standby function ensures that the temperature display continues to operate when the heater is switched off Connections Mains connection 230 V single phase AC 50 60 Hz 320 570 W depending on the model RS232 interface Technical data Temperature deviation spatial 1 C temporal lt 0 5 C Weight 40 75 kg depending on model Accessories Recirculating incubators upon request Standards Protection class 3 1 DIN 12 880 parts 1 2 Models with natural convection Shelves supplied Type Inner volume I _ WxDxH ext mm WxDxH int mm max Pk Cat No B6 73 552x540x700 408x344x459 2 9 1 390 1310 B 12 131 696x540x850 552x344x610 2 14 1 390 1320 B 20 233 754x720x910 610x514x672 2 16 1 390 1330 Tel 0800 22 33 44 freephone Email uksales uk vwr com 390 5 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Incubators Models with forced convection Shelves supplied Type Inner volume I WxDxH ext mm WxDxH int mm max Pk Cat No UB 6 64 552x540x700 408x344x459 2 9 1 390 0037 UB 12 112 696x540x850 552x344x610 2 14 1 390 0038 UB 20 210 754x720x910 610x514x672 2 16 1 390 0039 Shelves for Function Line Description Pk Cat No Shelf stainless steel for B 20 and UB 20 1 390 1311 Shelf stainless steel for B 12 and UB 12 1 390 1321 Shelf s
133. iry date and the full designation are printed on each tube Description Pk Cat No Wood cotton 100 710 0181 Aluminium alginate 100 710 0184 Plastic alginate 100 710 0429 Aluminium viscose 100 710 0430 e Wood cotton charcoal 100 710 0456 Plastic viscose 100 710 0457 Wood carded cotton 100 710 0458 Twisted aluminium viscose 100 710 0461 e pn Plastic polyester ATP free 100 710 0462 Fha Simple swabs in peel packs e Individually sterile packed e Available in various combinations i Description Pk Cat No g Plastic polyester 1000 710 0459 ri Plastic viscose 2 per peel pouch 1000 710 0460 4 Plastic minitip viscose 100 710 0463 bat Plastic minitip polyester 100 710 0464 Ta Wood cotton 1000 710 0185 HA Plastic viscose 1000 710 0186 g Aluminium viscose 1000 710 0187 SRK is an acronym for Swab Rinse Kits Swabbing is one of oldest and most widely used methods for microbiological examination of surfaces When swabs are used together as a kit with a tube of solution to pre moisten the swab before sampling and then to transport and rinse the swab after sample collection it is an effective method for the detection and quantification of microorganisms SRK Rinse Solution is an isotonic salt solution with additional substances to neutralise and inactivate disinfectants and sanitising agents e Ideal for quantification of microorganisms with transport at room tempe
134. isit us at http eu vwr com e business Email uksales uk vwr com 2 50 Web http uk vwr com 392 Cell Culture Cell Culture Larger Scale Bioprocessing Cell Culture Chambers Corning CellSTACK Corning The Corning CellSTACK Culture Chambers are available in five sizes and with three different surface treatments e Two 26 mm diameter filling ports allow direct access to chamber bottom providing greateer flexibility for sterile filling and emptying by pouring pipetting or via tubing in a fully closed system e Standard 33 mm threaded caps have 0 2 um pore non wettable membranes sealed directly to the caps to allow gas exchange while minimising the risk of contamination e Optional 33 mm threaded caps are available with integrally sealed USP Class VI certified C Flex tubing to allow direct sterile transfer of media and cells via pumping or gravity feed e Polystyrene construction provides excellent optical clarity and mechanical strength TC treated surface Uncharged polystyrene has an uncharged hydrophobic surface to which cell attachment proteins bind poorly This results in poor and uneven cell attachment and growth Tissue culture TC treated polystyrene has a negatively charged hydrophilic surface to which cell attachment proteins bind evenly This provides a good surface for cell attachment and growth Type Growth area cm _ Pk Cat No 1 Chamber 636 8 734 1038 2 Chamber 1272 5 734 1039 5
135. isted wire 100 720 0137 Wood stick peel pouch 1250 720 0127 Wood stick 5 pouch 2500 720 0128 Plastic stick peel pouch 1250 720 0129 Plastic stick fine tip peel pouch 125 720 0130 Wood stick bulk nonsterile for autoclaving 5000 720 0131 Wood stick bulk sterile 5000 720 0132 VWR E53 www vwr com Sampling Bags and Swabs Sponge Swabs Polywipe environmental and hygiene monitoring Available dry or pre moistened with sterile diluent Polywipes are supplied in peel pouches for immediate use or in a 110 ml blue capped container for transfer to the laboratory Non inhibitory sponge material allows effective sampling of larger areas for seme e Blue colour for visibility e Triple wrapped and radiation sterilised Delivery Information Supplied with individual sterile glove for handling Description Pk Cat No olywipe ml container l Polywipe peel pouch 50 720 0145 Swabs for Transport Transwab Each transport tube contains 5 ml of Amie s clear or Amie s charcoal medium which enables deep sample immersion e Excellent recovery of both aerobic and anaerobic bacteria Delivery Information Each Transwab unit consists of a sterile easy peel pack containing a plastic duo or wire applicator with colour coded cap and prelabelled transport tube Description Pk Cat No Amies charcoal 125 720 2201 Amies clear 125 720 2202 Plain medium 125 720 0138 Clear wet medium 125 720 0141 Charcoal medium 125 720
136. ith temperature safety device Class 3 1 Connections RS422 interface for communication software mains connection 230 V 50 60 Hz Technical data Temperature deviation 0 1 C rated output 1000 W CB 53 1300 W CB 150 1500 W CB 210 Calibration Calibrations and validations possible Supplied with Binder test certificate Accessories A wide range of options and accessories is available including partitioned inner glass door and divided shelves CB 150 and CB 210 only O2 control Ethernet interface connection kit for CO2 O2 N2 Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No CB 53 COz incubator 53 580x545x720 400x330x400 1 390 0319 CB 53 COz incubator with O2 control 53 580x545x720 400x330x400 1 390 0320 CB 150 COz incubator 150 680x722x919 500x500x600 1 390 6054 CB 150 COz incubator with O2 control 150 680x722x919 500x500x600 1 390 6056 CB 150 COz incubator with O2 control with gas tight divided inner glass door 150 680x722x919 500x500x600 1 390 6060 and divided shelves CB 150 COz incubator with gas tight divided inner glass door and divided 150 680x722x919 500x500x600 1 390 6058 shelves CB 210 CO2 incubator 210 740x722x1069 560x500x750 1 390 6055 CB 210 CO2 incubator with O2 control 210 740x722x1069 560x500x750 1 390 6057 CB 210 COz incubator with gas tight divided inner glass door and divided 210 740x722x1069 560x500x750 1 390 6059 shelves 390 18 VWR
137. itro system for the study of cell invasion through the basement membrane It consists of BD Falcon Cell Culture Inserts containing an 8 0 um pore size PET membrane coated with a uniform layer of BD Matrigel Matrix The BD BioCoat Tumour Invasion System is an in vitro system for the study of tumour cell invasion through the basement membrane It consists of BD Falcon FluoroBlok Multiwell Insert Plates containing an 8 0 um pore size FluoroBlok membrane coated with a uniform layer of BD Matrigel Basement Membrane Matrix The BD Matrigel Matrix occludes the pores of the FluoroBlok membrane blocking non invasive cells from migrating through the membrane Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 30 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Inserts e Proven biological performance using BD FluoroBlok PET membrane coated with BD Matrigel Matrix e Allows for rapid and reproducible quantitation of tumour cell invasion in vitro e Increase throughput for tumour cell invasion assays e Allows automation of assays with simplified and non destructive fluorescence detection Saves time and labour screening for prospective anti metastatic compounds Tested for the ability to allow invasion of HT 1080 cells an invasive human fibrosarcoma cell line and to Quality control exclude invasion of 3T3 cells a non invasive mouse fibroblast cell line Tested and found negative for bacteria and
138. ksales uk vwr com 391 5 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Petri Dishes Petri Dishes with Absorbent Pads Life Sciences PS absorbent pads cellulose sem Ideal for microbiological analysis when performing the Membrane Filter MF Technique Filter size accepts 47 mm membrane filter Gamma irradiated for sterilisation no dangerous EtO residuals e Easy to use opens easily with one hand yet closes to a tight seal e Uses less space on the lab bench or in the incubator with easy stacking base Height Version mm mm _ Sterile Pk Cat No Polystyrol 47 9 100 516 8020 g Petridish w o absorbent 50 9 500 516 8021 pads Petridish w o ab t etridish w o absorben 50 9 F 100 516 8029 pads Petri dishes with 50 9 100 516 8032 absorbent pads Bioassay Dishes Nunc PS sterile with lid Useful for screening large numbers of colonies in cloning experiments Low profile dishes designed for genomic screening and colony picking e Can be used for culturing bacteria and fungi also suitable in agar diffusion assays e Can be used as a moisture chamber for incubation of MicroWell plates e Lined with wet filter paper can also be used as an incubation chamber for in situ hybridisation e Large growth area e Low profile dish fits robotic instruments Recommended working volume Description ml WxDxH ext mm Pk Cat No Bioassay dishes 500 cm surface et 225 245x24
139. l cell tubulogenesis which saves time and improves reproducibility Description Pk Cat No One 96 well black clear bottom Optilux microplate 1 734 1022 Five 96 well black clear bottom Optilux microplates 5 734 1023 BD Human Umbilical Vein Endothelial Cells Pre qualified primary endothelial cells ensure assay performance and data reproducibility Description Pk Cat No HUVEC 2 gt 5x105 cells 1 734 1120 Cell Culture Inserts Nunc thermally welded to the polystyrene upper structure Inserts with 0 02 um Anopore membrane have maximum clarity for microscopy are non autofluorescent and highly porous Polycarbonate membranes in larger pore sizes are adaptable to many uses in cell culture including transport studies toxicity tests chemotaxis studies and electron microscopy Both membrane types are transparent when wet and are suitable for phase contrast and Normanski Optic Systems For cultivation of most cell types without matrix coating Membranes are seme e Treated and quality controlled for cell culture e Excellent cell attachment and growth e Non toxic and resistant to most solvents e Low non specific binding e Polycarbonate membrane inserts also available pre packed in multidishes Anapore is a trademark of Whatman Scientific Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 32 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Inserts
140. l fi I li 4 ID barbed T cap emale MPC coup ing 6 4 mm ID barbe 4 734 4062 fitting with male end cap sterile 9 3 M cap fema e MPC coup ing 9 5 mm ID barbed 4 734 4063 fitting with male end cap sterile Fill cap male MPC coupling 6 4 mm ID barbed fitting with female end cap sterile Fill le MP li D ID barbed cap male MPC coupling a mm arbe 4 734 4068 fitting with female end cap sterile Stacking device ABS non sterile 5 734 4064 5 734 1109 4 734 1209 5 734 1110 4 734 4066 All caps are 33 mm threaded caps Cell Factories EasyFill Nunclon A Nunc Tel 0800 22 33 44 freephone Fax 01455 55 85 86 PS sterile The EasyFill Cell Factory has one large and one small opening in each unit which makes it versatile and easy to use EasyFill Cell Factory bridges the gap between small scale research and large scale GMP production It can be used as it is without any accessories or used with plug and play connections for rapid attachment of tubing and filters significantly reducing contamination risk EasyFill systems are available with one two four or ten growth chamber levels providing surface areas ranging from 630 cm to 6300 cm e Versatile large opening facilitates the desire to pour media directly and small opening supports those that need a closed aseptic system for filling and harvesting e Bridges small scale process development with large scale production e E
141. l grade adhesive for tissue culture plates bio blocks and 96 well plates where gas exchange is w necessary for cell or bacterial growth These rayon films minimise cross contamination spillage and evaporation They allow uniform air and CO2 exchange for all wells unlike plate lids which favour exchange for wells near plate edges Sterile product is packaged in tamper evident bags of 25 e Non cytotoxic highly gas permeable e Pierceable with tips or pipettes for sample recovery e Recommended for temperatures from 20 C to 80 C Each film LxD 142 9x82 6 mm for standard size tissue culture plates Description Pk Cat No Rayon films non sterile 100 391 1261 Rayon films sterile 50 391 1262 Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 56 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Accessories and Sealing Systems Pierceable Films for Robotics Clear Zone VWRED o w Description Addition of a die cut white polypropylene sub layer provides these polyethylene films with an adhesive free zone above each well for w applications where adhesive fouling of pipette tips or robotic probes is a concern Suitable for 96 well plates only e Clear zone above each well is free of adhesive e Minimise sample exposure to adhesive Pk Cat No Clear Zone films non sterile 50 391 1264 g Clear Zone films sterile 50 391 1265 Precut Pierceable Vin
142. lation throughout the flask while keeping the beads in suspension e Two large side arms with screw cap closures allow easy sampling e Units 500 ml and larger have 45 mm side arms to be used as an air vent media inlet or outlet inoculation port pH probe etc All flasks have been proportioned to provide a headspace of 1 1 or greater e Removable stainless steel pin immobilises the impeller during handling or decanting to prevent damage to cells or microcarriers e Size 125 to 1000 ml are graduated in 50 ml increments sizes 3000 to 6000 ml are graduated in 500 ml increments Capacity ml xH mm Cap size Pk Cat No topo 51 400 125 65x155 bra o lateral 33 1 734 3000 430 topo 51 400 250 85x175 bra o lateral 33 1 734 3001 430 t 100 500 110x190 opo 100400 4 734 3002 bra o lateral 45 t 100 1000 130x250 opo 100 400 4 734 3003 bra o lateral 45 topo 100 400 3000 178x341 1 734 3004 bra o lateral 45 6000 258x404 OPO 100 400 4 734 3005 bra o lateral 45 Replacement Flasks for Celstir and Magna Flex Wheaton Description Pk Cat No 25 ml Celstir flask only 1 734 3169 50 ml Celstir flask only 1 734 3170 125 ml Celstir Magna Flex flask only 1 734 3171 250 ml Celstir Magna Flex flask only 1 734 3172 500 ml Celstir Magna Flex flask only 1 734 3173 1000 ml Celstir Magna Flex flask only 1 734 3174 3000 ml Celstir Magna Flex flask only 1 734 31
143. ld This is a proprietary mineralised calcium phosphate bioceramic that is ideal for in vitro and in vivo analysis of bone metabolism and cartilage regeneration Applications include promotion of cell growth and differentiation e g MC3T3 E1 osteoblasts stationary cell cultivation in Multiwell plates dynamic cell seeding in larger vessels e g BD Falcon 50 ml conical tubes bone remodeling studies jn vitro and in vivo and non invasive evaluation of cell growth using the BD Oxygen Biosensor System Description Pk Cat No BD 3D Calcium Phosphate Scaffold 24 734 1063 BD 3D Collagen Composite Scaffold This is a natural scaffold manufactured from a proprietary mixture of collagens that are derived from bovine hide Overall this material exhibits collagen fibrillar architecture which is representative of the structure of collagen within the interstitial matrix Applications include promotion of cell growth and differentiation e g MC3T3 E1 osteoblasts WI 38 human lung fibroblasts primary rat hepatocytes ECM pre incubation for specific stem progenitor cell differentiation assays dynamic cell seeding in larger vessels e g BD Falcon 50 ml conical tubes and non invasive evaluation of cell growth using the BD Oxygen Biosensor System Description Pk Cat No BD 3D Collagen Composite Scaffold 24 734 1061 BD 3D OPLA Open Cell Polylactic Acid Scaffold This is a synthetic polymer scaffold that is synthesized f
144. led vented 50 734 1723 Rectangular TC treated angled phenolic 50 734 1726 Rectangular TC treated with bar code angled vented 84 734 1733 g Rectangular CellBIND surface with bar code angled vented 84 734 1214 Rectangular CellBIND surface angled vented 50 734 0093 Rectangular CellBIND surface angled phenolic 50 734 1206 225 cm growth area flasks Description Neck Cap Pk Cat No Traditional TC treated angled plug seal 25 734 1724 Traditional TC treated angled vented 25 734 1725 g Rectangular TC treated canted phenolic 24 734 1519 Rectangular TC treated canted vented 24 734 1520 Traditional CellBIND surface angled vented 25 734 0094 235 cm growth area flasks Description Neck Cap Pk Cat No Rectangular CellBIND surface with bar code angled vented 42 734 4192 1720 cm growth area HYPERFlask vessels Description Pk Cat No HYPERFlask with CellBIND surface bar coded 4 734 4009 HYPERFlask with CellBIND surface bar coded 24 734 4010 BD Primaria Cell Culture Flasks BD Biosciences PS tissue culture treated sterile non pyrogenic The complex surface on BD Primaria products is homogeneous and stable and is used to improve attachment and differentiation of a variety of cell types The surface chemistry of BD Primaria products is confirmed by Electron Scanning for Chemical Analysis ESCA e Unique nitrogen containing tissue culture surface chemistry improv
145. ll holds 10 ml For research use only e 45 slope funnels oocyte embryo into centre of well e Stand off rim on base protects optical surface from scratching e Treated for optimal cell attachment e Sterilised by gamma radiation e Certified non pyrogenic Description Pk Cat No IVF culture dishes 60x15 mm 500 734 1542 Chamber Slides BD Falcon CultureSlides for in situ Analysis BD Biosciences Soda lime glass slide PS vessel lid and tool BD Falcon CultureSlides allow cells to be cultured and then analysed ona glass microscope slide Cells are grown in a plastic chamber attached to a specially prepared microscope slide Cells can be fixed and stained in situ without disruption of the cell monolayer e Chamber is easily and safely removed using the disposable safety removal tool supplied e Pressure sensitive biocompatible acrylic adhesive gasket remains with the vessel after removal not on the slide facilitating further processing or placement of coverslips e Blue hydrophobic border defines cell culture areas e Wells numbered for easy identification e Trays designed for incubator use WxL Slide 25x75 mm with 1 2 mm bevelled edge Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 24 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Chamber Slides Recommended working Description volume ml Growth area cm Pk Cat No 1 Well Culture
146. llular activation e With stacking beads to aid handling e Supplied with vents to provide consistent gas exchange e Sterilised by gamma radiation e Certified non pyrogenic Recommende d working Culture area Description volume ml cm Pk Cat No Dishes 35x10 mm CellBIND surface 1 8 2 7 8 210 734 4055 Dishes 35x10 mm TC treated surface 1 8 2 7 8 500 734 1698 Dishes 60x15 mm CellBIND surface 4 2 6 3 21 126 734 4056 Dishes 60x15 mm TC treated surface 4 2 6 3 21 500 734 1699 e Dishes 60x15 mm Ultra Low Attachment surface 4 2 6 3 21 20 734 0884 Dishes 60x15 mm with 2 mm grid TC treated surface 4 2 6 3 21 500 734 1703 Dishes 100x20 mm Ultra Low Attachment surface 11 16 5 55 20 734 0885 Dishes 100x20 mm CellBIND surface 11 16 5 55 40 734 4057 Dishes 100x20 mm TC treated 11 16 5 55 500 734 1815 Dishes 100x20 mm TC treated in 6 pack carriers 11 16 5 55 480 734 1705 Dishes 150x25 mm TC treated 30 4 45 6 148 60 734 1711 Dishes square 245x25 mm TC treated 100 150 500 16 734 1727 Interior dimensions 224x224 mm Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 20 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Plates and Dishes Cell Culture Dishes Untreated Corning Optically clear PS For applications where cell attachment is not desired e With stacking beads to aid handling e Supplied with vents to pr
147. lour Culture area cm Well volume Pk Cat No Standard plate with U bottom transparent 0 32 330 ul 50 735 2082 Standard plate with F bottom transparent 0 32 350 ul 50 735 2083 Standard plate with U bottom white 0 32 330 ul 50 735 2084 Standard plate with F bottom white 0 32 350 ul 50 735 2085 Standard plate with C bottom white 0 25 350 ul 50 735 2086 Standard plate with U bottom black 0 32 330 ul 50 735 2087 Standard plate with F bottom black 0 32 350 ul 50 735 2088 Standard plate with C bottom black 0 25 350 ul 50 735 2089 Transparent base with F bottom white 0 31 330 ul 50 735 2090 Transparent base with F bottom black 0 31 330 ul 50 735 2091 384 Well HTS Microplates for Cell Culture BRANDplates Brand cellGrade PS solid or with transparent base These flat bottom plates are available in different colours and with standard or transparent base formats The standard surface for cultivation of adherent cell lines Chemical groups on the surface of the plate bind to serum compounds stimulating the growth of immobilised cells Well volume Culture area Description Colour l cm Pk Cat No Standard plate with F bottom transparent 100 0 12 50 735 2105 Standard plate with F bottom white 100 0 12 50 735 2106 Standard plate with F bottom black 100 0 12 50 735 2107 Standard plate with F bottom low volume transparent 30 0 07 50 735 2108 Standard plate with F bottom low volume white 30 0 07 50 735 2109 Standar
148. m chamber e Labyrinth lid condensation rings and deep well design control contamination reduce evaporation and minimise edge effects e Reliable vacuum gas plasma tissue culture treatment provides well to well and plate to plate consistency e Convenient peel open medical style packaging e Individual and Ready Stack RS trays are PET and recyclable Colour Clear Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 8 Fax 01455 55 85 86 Web http uk vwr com i Cell Culture Cell Culture Plates and Dishes Growth area Description Volume ml cm Packed Pk Cat No 6 Well TC treated flat bottom with lid 15 5 9 6 T tray 50 734 0019 e 6 Well TC treated flat bottom with lid 15 5 9 6 6 bag 36 734 0054 6 Well TC treated flat bottom with lid 15 5 9 6 10 RS tray 60 736 2025 12 Well TC treated flat bottom with lid 6 3 8 T tray 50 391 0006 12 Well TC treated flat bottom with lid 6 3 8 6 bag 36 734 0055 24 Well TC treated flat bottom with lid 3 5 2 T tray 50 734 0020 24 Well TC treated flat bottom with lid 3 5 2 6 bag 36 734 0056 48 Well TC treated flat bottom with lid 1 4 0 75 T tray 50 734 0028 g 48 Well TC treated flat bottom with lid 1 4 0 75 6 bag 36 734 0058 24 Well TC treated flat bottom with lid 3 5 2 10 RS tray 60 736 2026 96 Well TC treated flat bottom with lid 0 37 0 32 14 RS tray 84 736 2027 Well treated flat b
149. ma treatment which promotes cell attachment The hydrophilic tissue culture surface contains a variety of negatively charged functional groups that support cell attachment and spreading The design and frosted rim of the Easy Grip dish improves handling of small dishes The ability to pick up a small dish conveniently without accidentally removing the lid allows faster working and improves aseptic manipulation e Flat and optically clear for distortion free microscopic visualisation of cells e Lids designed for optimal gas exchange e Stacking rings allow for easier stacking and handling e Cell performance tested to ensure consistent results Recommended working Culture area Description volume ml cm Pk Cat No Easy Grip dishes 35x10 mm 2 5 3 0 11 78 500 734 0005 entre Well organ culture dish 60x15 mm Easy Grip dishes 60x15 mm 6 0 7 0 19 5 500 734 0007 Standard dishes 60x15 mm 6 0 7 0 21 29 500 734 0961 tandard dishes x20 mm 0 17 Dishes with 20 mm grid moulded in base 150x25 mm 45 0 50 0 156 36 100 734 0013 Cell Culture Dishes Treated Corning 734 1703 734 1815 734 1727 Optically clear PS Available with a choice of treated surface Corning CellBIND Surface increases surface wetability for more even and consistent cell attachment Ultra Low Attachment dishes feature a covalently bound hydrogel layer that minimises cell attachment protein absorption and ce
150. meters and provides trend analysis for convenient evaluation of the unit s performance The control is fitted on the door to ensure it is easily accessible and clearly visible Choice of several languages Any changes made to the cultivation conditions are displayed directly via protocols and user recordings on the screen Glass doors have safety interlocks which ensure that the inner doors are not left open accidentally Shelves Supplied with 3 stainless steel or solid copper shelves 10 or 12 shelf positions depending on model Cleaning and disinfection Innovative ContraCon 90 C decontamination routine using moist heat ensures a simple and reliable cleaning process Safety Solid copper interior fitting verifiably prevent the growth of bacteria and fungi by natural means Visual alarm signals on the display Control Short humidity recovery times Units have a large directly heated water reservoir as well as up to five times quicker humidity recovery times than conventional incubators with a water tank Audible and visual alarms when water level is low Optional O2 control unit with Auto Cal for applications that require hypoxic or hyperoxic conditions The HERAcell i series offers two optional O2 control ranges Humidity Constant humidity 95 3 RH Connections Mains connection 230 V 50 60 Hz Technical data Temperature deviation 0 1 C nominal power 580 W 150i 640 W 240i Accessories Available with optional TC COz or IR COz
151. n top of each other to save space Shelves Supplied with 2 chrome plated racks Cleaning and disinfection Hot air sterilisation at 100 C Safety Independent adjustable temperature safety device Class 3 1 DIN 12880 with visual and acoustic alarm Controller Electronically controlled APT line preheating chamber and DCT refrigeration system Microprocessor programme controller with LED display with 2 programmes each with 10 sections alternatively 1 programme with 20 sections and various timer functions temperature monitor Class 3 1 DIN 12880 elapsed time indicator Connections DataControl system or can be switched to printer with RS232 RS422 port converter adjustable printing intervals RS422 port for APT COM communication software RS 422 interface for communication software or switch over to printer output with RS 232 RS 422 interface converter nominal voltage 230 V 390 8 VW R E F www vwr com Cell Culture Incubators Calibrations and validations possible Supplied with Binder test certificate Technical data Temperature fluctuation 0 1 C Nominal power 460 W KB 53 930 W KB240 1350 W KB 720 Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No KB 23 20 433x516x618 222x277x330 1 390 6076 KB 53 53 634x576x837 400x330x400 1 390 6080 KB 115 115 834x646x1022 600x400x480 1 390 6081 KB 400 400 884x716x1850 650x470x1308 1 390 6083 KB 720 700 1234x867x1816 1000x600x1168 1 3
152. ned in the flat sided tube in the horizontal position e Excellent optical quality e Certified surface treatment for optimal cell attachment and growth Description Recommended working volume ml Pk Cat No Round tubes with screw cap 100x14 mm T 600 734 2037 Round tubes with push on cap 100x13 mm T 600 734 2036 Flat sided tubes with screw cap 110x16 mm 3 450 734 2068 Please contact your local VWR sales office or go to wwwawrcam for the latest news and special offers 392 41 VWR E oY Www vwr com Cell Culture Cell Culture Bottles and Tubes Disposable Tubes Nunc PS or PP non sterile PS tubes are suitable for a broad range of applications whilst PP tubes are particularly useful in serology where the low adsorption of proteins is of great importance e Graduation marked e PS tubes are transparent e Stoppers or snap caps sold separately please enquire for more information Capacity Description ml xH mm Pk Cat No PS round bottom 4 11x70 3600 734 0493 e PP round bottom 12 15x100 1200 525 0054 PS conical bottom 14 17x110 1800 525 0060 Culture Tubes with Screw Neck Pyrex borosilicate glass Ideal for use with samples sensitive to leaching from plastic tubes e Without screw caps e High resistance to temperature and chemicals e Autoclavable at 121 C Description Capacity ml xH mm Pk Cat No 7 9 13x100 250 212 7498
153. ng Systems Sealing Film AxySeal Polyester based with uniformly and consistently applied acrylic adhesive to eliminate the edge effect in ELISA assays Suitable for sealing tissue culture plates for short term storage and incubation for transport and the containment of biohazardous solutions e No edge effect in ELISA assays e Functional temperature range of 40 to 104 C Description Pk Cat No Sealing film 100 732 7506 e Sealing Mats BugStopper Whatman cultures being grown in a 24 well microplate This reusable autoclavable paesan sterile closure which is produced using chemically resistant bio safe silicone e N rubber incorporates hydrophobic microfilters which provide an ideal vent for each well and eliminates well to well cross contamination More efficient than plastic lids testing has confirmed that Bugstopper sealing mats improve cell growth and significantly reduce evaporation making them ideal for extended growth of slow growing bacteria and fungi Bugstopper sealing mats provide a simple and reliable method for venting seme Repeated autoclave cycles do not affect gas exchange or retention capabilities we gt e Rated 99 9 efficient for bacteria and viruses by restricting microorganisms while allowing Oz and COz to pass through the membrane Silicone rubber reseals after puncture thus keeping cultures sterile during inoculation or aspiration Description Pk Cat No
154. nsions or stem cells Well volume Culture area Description Colour ul cm Pk Cat No Standard plate with F bottom transparent 100 0 12 50 735 2092 Standard plate with F bottom white 100 0 12 50 735 2093 Standard plate with F bottom black 100 0 12 50 735 2094 Transparent base with F bottom white 120 0 13 50 735 2095 Transparent base with F bottom black 120 0 13 50 735 2096 1536 Well HTS Microplates for Cell Culture BRANDplates Brand PS Cell culture treated flat bottomed plates cellGrade The standard surface for cultivation of adherent cell lines Chemical groups on the surface of the plate bind to serum compounds stimulating the growth of immobilised cells Description Colour Well volume yl __Culture area cm Pk Cat No Standard plate with F bottom transparent 10 0 02 50 735 2113 Standard plate with F bottom white 10 0 02 50 735 2114 Standard plate with F bottom black 10 0 02 50 735 2115 Lids for BRANDplates Brand Description Pk Cat No With condensation rings for standard 96 well plates 100 732 1131 Without condensation rings for 96 well plates 100 732 1132 Without condensation rings for 384 well plates 50 732 1133 Without condensation rings for 1536 well plates 50 732 1134 Please contact your local VWR sales office or go to wwwawrcam for the latest news and special offers 392 13 VW R E F www vwr com 96 Well Cell Culture Plates Corning PS clear with l
155. nsulin and steroid hormones It can be used to culture a variety of cells under serum free or serum reduced conditions Lyophilised from a solution of Dulbecco s Phosphate Buffered Saline DPBS containing ECGS EGF Formulation insulin human transferrin triiodotryronine progesterone oestradiol 17 amp testosterone hydrocortisone selenous acid and o phosphorylethanolamine reconstitute in 5 ml distilled H2O stock solution Tested for ability to promote proliferation of BALB c 3T3 cells in serum reduced medium Filtered 0 2 um membrane and tested and found negative for bacteria fungi and mycoplasma Recommended concentration 1 1000 dilute stock solution in serum reduced medium Stable for at least three months at 2 8 C lyophilised or 3 months at 20 C solubilised Avoid multiple freeze thaw cycles Quality control Storage and stability Description Pk Cat No BD MITO 5 ml 734 1317 Serum Replacement Nu serum BD Biosciences BD Nu serum growth media supplement provides a low protein alternative to newborn calf foetal bovine and other sera routinely used for cell culture The low protein content facilitates protein purification virus production monoclonal antibody production and screening and increases the frequency of successful transfection of cells Frozen solution containing 2 5 foetal bovine serum EGF ECGS insulin human transferring triiodothyronine progesterone oe
156. o Start up kit sterile 1 Kit 734 2106 Connector Teflon 10 734 2110 Cover caps 400 734 2143 Filter 10 391 8339 BD Falcon Cell Strainers BD Biosciences suspension Made of a strong nylon mesh and available with 40 70 or 100 um pores that are evenly spaced for optimal performance in a variety of applications A faster and easier alternative to gauze filtration in procedures involving dissociation of cells from either clumps or primary tissue An easy ready to use way to consistently obtain a more uniform single cell seme e Moulded colour coded PP frame with tab enables easy handling e Fits perfectly into a 50 ml BD Falcon conical tube or other similarly sized tube Sterilised by gamma irradiation and conveniently available in individual peel open packaging e Extended lip on strainer enables aseptic handling with forceps Description Pk Cat No Cell strainers 40 um pore size blue frame 50 734 0002 Cell strainers 70 um pore size white frame 50 734 0003 e ell strainers um pore size yellow frame l BD Falcon Cell Scrapers BD Biosciences PS handle TPE blade Designed to provide maximum accessibility to the growth surfaces of a variety of culture vessels Cross ribbed PS handle provides greater rigidity to ensure better control while scraping cells e Highly compliant TPE blade pivots to provide multiple angles to remove cells from the entire growth surface
157. o easy to access partitions e Place on the counter or mount on a wall e Solid and clear e Mounting screws included WxDxH 203x102x229 mm Pk Cat No 1 391 1165 g Poxygrid Not suitable for glass Petri dishes e Easily loaded and emptied from the top e Epoxy coated steel wire rack e For plastic Petri dishes 90 and 100 mm WxDxH 108x108x356 mm 1 stack for 20 dishes WxDxH 343x105x305 mm 3 stacks for 42 dishes Description Pk Cat No 1 Stack 1 391 2011 g 3 Stacks 1 391 2013 g Tel 0800 22 33 44 freephone Email uksales uk vwr com 391 7 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Innoculating Needles and Accessories Inoculating Loops in Resealable Bags VWRED The resealable bags can be easily opened with one hand The loops and needles are placed upside down in the bag in order to guarantee purity as yy long as possible The loops are colour coded and rigid or flexible versions are available Optional holder for bags is available seme e Gamma sterilised e Packaged in resealable bags e The handle has a hexagonal shape for easy and secure handling Packaging 40 loops in a bag 25 bags per pack Delivery Information Delivered with a certification of calibration in each box Description Capacity ul Colour Packed Pk Cat No beans rigid 1 dark green 40 1000 612 9360 g ps ng T0 dark blue 40 1000 612 9359 5 F ight green Loops flexible
158. ons for accuracy e Vent cap option for continuous gas exchange while ensuring sterility and e Individually packaged and radiation sterilised for ease of use Corning baffled and plain Erlenmeyer flasks are ideal for shaker culture seme Type Capacity ml Neck Cap Pk Cat No Erlenmeyer flask baffled 125 26mm plug seal 50 734 4200 Erlenmeyer flask baffled 125 26 mm vented 50 734 4201 Erlenmeyer flask baffled 250 31mm plug seal 50 734 4202 Erlenmeyer flask baffled 250 31mm vented 50 734 4203 Erlenmeyer flask baffled 500 43 mm plug seal 25 734 4204 Erlenmeyer flask baffled 500 43 mm vented 25 734 4197 Erlenmeyer flask baffled 1L 43 mm plug seal 25 734 4198 Erlenmeyer flask baffled 1L 43 mm vented 25 734 4199 Erlenmeyer flask baffled 2L 48 mm vented 6 734 1905 Erlenmeyer flask Fernbach design baffled 3L 70mm vented 4 734 1903 Plain bottom Erlenmeyer Flasks Type Capacity ml Neck Cap Pk Cat No Erlenmeyer flask 125 26 mm plug seal 50 734 1832 Erlenmeyer flask 125 26 mm vented 50 734 1885 Erlenmeyer flask 250 31mm plug seal 50 734 1820 Erlenmeyer flask 250 31mm vented 50 734 1886 e Erlenmeyer flask 500 43 mm plug seal 25 734 1833 g Erlenmeyer flask 500 43 mm vented 25 734 1887 Erlenmeyer flask AL 43 mm plug seal 25 734 1888 Erlenmeyer flask 1L 43 mm vented 25 734 1889 Erlenmeyer flask 2L 48 mm vented 6 7
159. other imperfections Version mm Height mm Pk Cat No 40 12 1 391 2805 e 60 15 1 391 2810 80 15 1 391 2820 100 10 1 391 2831 100 15 1 391 2830 5 Petri dish 100 20 1 391 2840 e 120 20 1 391 2850 e 10 25 1 3912800 gt 180 30 1 391 2870 200 30 1 391 2880 CrystalPS plates specially adapted to the needs of all users All plates are manufactured under aseptic conditions according to the cleanroom technology e Perfect flatness to ensure the agar s homogeneity e Stable stacking e Retains shape up to 55 C high level of mechanical resistance e Compatible with the most industrial automatic agar pouring machines Height Version mm mm Sterile Pk Cat No 100 14 a 500 391 2056 With 3 vents 70g 14 z 500 391 2057 z With 1 vent 90 14 2 825 216 0509 e This plate is used in the pharmaceutical or food processing industry and also in hospital environments for surfaces such as skin operating tables and work surfaces e The moulded grid at the bottom makes it possible to identify contamination per cm2 e The unique closing system grid lid ensures safety during transport e The domed base guarantees a saving in agar while maintaining the surface sampling e Stable stacking Cat No 391 2064 e Version With 3 vents VWRED mm Height mm Pk 65 14 5 720 www vwr com Cell Culture Petri Dishes Petri Dishes Duroplan DURAN Group
160. ottom with li ag 96 Well TC treated round bottom without lid 0 32 0 36 1 tray 50 734 0026 96 Well plate lid 1 bag 50 734 0022 6 Well non treated flat bottom with lid 15 5 9 6 T tray 50 734 0948 24 Well non treated flat bottom with lid 3 5 2 1 tray 50 734 0949 Multiple Well Cell Culture Plates Costar Corning 734 1599 Clear PS flat bottom with lid Available with a choice of surface treatments 734 1598 e Non reversible lids with condensation rings to reduce contamination e Uniform footprint for ease of stacking e Individual alphanumeric codes for well identification e Sterilised by gamma radiation e Certified non pyrogenic Description Pk Cat No 6 Well plates TC treated individually wrapped 50 734 1599 6 Well plates TC treated 100 734 1596 6 Well plates Corning CellBIND surface 50 734 1210 6 Well plates Ultra Low Attachment surface individually wrapped 24 734 1582 12 Well plates TC treated individually wrapped 50 734 1598 e 12 Well plates TC treated 100 734 1597 12 Well plates Corning CellBIND surface 50 734 1211 24 Well plates TC treated individually wrapped 50 734 1605 24 Well plates TC treated individually wrapped 100 734 1604 24 Well plates TC treated 100 734 1606 24 Well plates Corning CellBIND surface 50 734 1212 24 Well plates Ultra Low Attachment surface individually wrapped 24 734 1584 48 Well plate
161. ovide consistent gas exchange e Sterilised by gamma radiation e Certified non pyrogenic Recommended working Description volume ml Culture area cm Pk Cat No Dishes 35x10 mm 1 8 2 7 9 500 734 1707 Dishes 60x15 mm 4 2 6 3 21 500 734 1708 Dishes 100x20 mm 11 16 5 55 500 734 1709 Dishes 150x25 mm 30 4 45 6 152 60 734 1710 Dishes square 224x224 mm 100 150 ml 500 16 734 1728 BioAssay Dishes 245 mm Square Corning PS with lids Designed with a stacking bead so that they will stack securely without slipping e Compatible with automated colony picking instruments e Certified non pyrogenic Description Pk Cat No BioAssay dish 18 mm deep untreated 16 734 1731 BioAssay dish 12 5 mm low profile untreated 20 734 1732 Cell Culture Dishes Nunclon A Nunc PS except 734 2113 made of Permanox sterile with lid seme A large range of dishes which have been tissue culture treated except 734 2109 suitable for suspension cultures which is not treated e Optically clear and uniform surface suitable for microscopy 60 mm and 40 mm dishes available with grids for cloning or determination of plating efficiency e Nunclon A certified except 734 2109 392 21 VWR E53 www vwr com Cell Culture Cell Culture Plates and Dishes Recommended Description working volume ml Growth area cm Pk Cat No 35x10 mm without grid or air
162. ower the risk of cross contamination e Flat bottom wells allow optimum optical quality e Nunclon A certified Description volume ml Growth area cm Pk Cat No 4 Well multidish 66x66 mm 1 0 q 120 734 2176 T 3 0 9 6 75 391 8036 6 Well multidish 128x86 mm 30 J6 35 734 0991 12 Well multidish 128x86 mm 2 0 3 5 75 734 2156 am 1 0 1 9 75 734 0992 e 24 Well multidish 128x86 mm TO 79 85 734 0993 gt T 0 5 cA 75 734 2157 48 Well multidish 128x86 mm 05 TT 35 734 1147 Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com Web http uk vwr com 392 10 Cell Culture Cell Culture Plates and Dishes OmniTrays Nunclon A Nunc PS sterile with lid Single well OmniTrays are ideal for a variety of uses e Standard microplate format with notched corners make them suitable for robotic handling e Certified surface treatment for optimal cell growth and attachment Culture area 84 cm External dimensions 128x86 mm Description Recommended working volume ml Pk Cat No OmniTray 90 60 732 2602 96 Well Microplates for Cell Culture BRANDplates Brand PS solid or with transparent base Plates are available with different well shapes round U bottom conical V bottom flat F bottom or C flat bottom with curved edges in different colours and with a choice of cell culture treated surfaces All BRANDplates are alphanumerically labelle
163. pplements is possible at any time by using the spare port which is covered with a Luer Lock connector e Centrifugation of the suspension can be carried out in the cultivation flask which has a format that fits a wide range of standard rotors Delivery Information Each pack contains two SuperSpinner D 1000 bioreactors Recommended working Description volume Pk Cat No SuperSpinner D 1000 200 800 2 432 0099 Culture Vessels Magnetic Nalgene 392 43 stirring bar and PP TFE stirring assembly Specially designed for efficient top to bottom mixing at low speed and low shear This lightweight break resistant 1 litre culture vessel is ideal for use on a magnetic stirring plate for small volume scale up applications Two magnetic stirring bars are included one small for bacteria and one large for mammalian cells Clear graduated PC vessel white PP screw caps Teflon TFE e Two shoulder access ports for easy removal of samples e Impeller height is adjustable e Thermal resistance 135 to 135 C autoclavable e Shatter proof with high mechanical strength Brim capacity 2 2 VWR E53 www vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Closure diameter top 63 mm Closure diameter side 38 430 Overall height 266 mm Outer diameter 137 mm Overall width including ports 190 mm Description Pk Cat No Culture Vessels Magnetic 1 734 5057 Probe Adapter Closures Nal
164. product are tested and found negative for bacteria and fungi Quality control HUVEC 2 cells are tested for migratory performance at a minimum of eight population doublings positive immunohistochemical staining for von Willebrand factor and CD31 antigen negative immunohistochemical staining to a actin positive Dil Ac LDL uptake and negative mycoplasma HIV 1 hepatitis B and C bacteria yeast and fungi BD BioCoat Angiogenesis Systems are stable for at least three months from the date of shipping when stored Storage and stability at 20 C HUVEC 2 cells should be stored in liquid nitrogen BD BioCoat Angiogenesis System Endothelial Cell Invasion For evaluation of endothelial cell invasion using real time fluorescence detection in a simplified and reproducible manner Description Pk Cat No One 24 Multiwell insert plate 1 734 1018 Five 24 Multiwell insert plates 5 734 1019 BD BioCoat Angiogenesis System Endothelial Cell Migration A quantitative and reproducible in vitro model system for examining the effects of prospective compounds on endothelial cell migration Description Pk Cat No One 24 Multiwell insert plate 1 734 1020 Five 24 Multiwell insert plates 5 734 1021 One 96 Multiwell insert plate 1 734 1118 Five 96 Multiwell insert plates 5 392 2504 BD BioCoat Angiogenesis System Endothelial Cell Tube Formation An optimised system for screening compounds that modulate endothelia
165. protocol manual OptiMag magnet 1 392 0601 OptiCell mailer kit Description Pk Cat No OptiCell mailer kit containing 5 OptiCell 1100 chambers 50 tips 5 OptiCell mailers shipping 4 392 0602 protocol manual OptiCell mailer 10 392 0603 Accessories Description Pk Cat No OptiCell rack for OptiCell 1100 and 2100 1 392 0604 OptiCell insertion tips 100 392 0605 OptiCell knife 3 392 0606 CELLine 1000 System BD Biosciences CELLine 1000 a membrane based disposable cell cultivation system guarantees high cell densities and is easy to use for recombinant protein expression and high yield monoclonal antibody MAb production e The CELLine system yields antibody concentrations comparable to that of ascites one CELLine flask can be used to produce as much antibody as 12 mice e Harvest volumes result in antibody concentrations that are 50 to 100 times higher than both roller bottles and tissue culture flasks Description Pk Cat No CELLine 1000 3 734 0389 CELLine is a trademark of Integra Biosciences Inc BD Falcon Multiwell Cell Culture Plates BD Biosciences PS sterile non pyrogenic All tissue culture treatments render polystyrene hydrophilic and result in the incorporation of a variety of anionic functional groups that support cell culture To ensure reproducible results and conditions all BD Falcon tissue culture treatment is performed in a vacuu
166. r formamide ethylene glycol 0 1 N NaOH or 0 1 N HCI at a concentration of gt 20 mg ml It is insoluble in methanol ethanol acetone benzene or chloroform In solution Gentamycin is very stable at 20 to 37 C Short autoclaving is possible It may be employed in the pH range from 2 2 and 10 without loss of activity Pk Cat No 1g A1492 0001 5g A1492 0005 10g A1492 0010 25g A1492 0025 Penicillin G Potassium Salt AppliChem Penicillin G was isolated from Penicillium notatum The bactericidal effect of this lactam antibiotic is based on the irreversible inhibition of transpeptidase interfering with the biosynthesis of the cell wall in bacteria Stability Penicillin G potassium salt is readily water soluble The dry substance can be stored at room temperature or 4 C and is stable for up to 5 years The buffered solution pH 4 5 7 loses 5 of its activity after 20 days at 4 C Storage of the solution at room temperature results in a 5 loss of activity after 48 hours 10 loss after 7 days and 20 50 loss after 14 days The stability in unbuffered solution is much lower approximately 3 7 days at 4 C ER Cat No 10g A1837 0010 25g A1837 0025 100g A1837 0100 Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 64 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Media and Supplements PCR Mycoplasma Test Kit AppliChem Ready to use PCR Mix for the detection
167. r and digital LED display for all set parameters and 7 day programme timer with real time clock e Integrated timer for temperature profiles of up to 40 ramps each segment adjustable from 1 min up to 999 h e Active control for humidifying and dehumidifying 20 95 RH with digital display of RH resolution 0 5 setting accuracy 1 e Various safety features including independent digitally adjustable electronic over temperature controller TWW protection Class 3 1 audible and visual alarm in case of over or under temperature over or under humidity door open or empty water tank Inner glass door enables samples to be viewed without affecting chamber temperature and prevents contamination Model HCP 108 HCP 153 HCP 246 Temperature range C Ambient 8 90 with humidity control Ambient 8 160 without humidity control Temperature fluctuation C 0 1 0 1 0 1 Temperature variation at 90 C C lt 0 3 lt 0 3 lt 0 3 Stability RH 1 5 1 5 1 5 Capacity l 108 153 246 Heating system Non turbulent ventilation system Shelves shelf positions 2 3 2 4 2 4 Internal WxDxH mm 560x400x480 480x500x640 640x600x640 External WxDxH mm 710x550x778 630x650x938 790x750x938 Weight kg 70 90 110 Nominal power W 1000 1500 2000 Ordering Information Units supplied with software memory card calibration certificate measurements taken at 60 C and 2 perforated stainless steel s
168. racy and fast temperature recovery time after the door has been opened e Adjustable fan speed BF series e Additional options and accessories Design Units up to 115 litres can be stacked on top of each other to save space Shelves Supplied with 2 chrome plated racks Cleaning Easy to clean design Residue free cleaning of entire inner chamber and inner glass door Safety Temperature safety device class 3 1 provides full protection against chamber over temperature with visual alarm Controller Electronically controlled APT line preheating chamber DS controller with integrated timer 0 to 99 h BD series MS controller with several timer functions such as Delayed OFF and Delayed ON BF series and adjustable ventilation by means of rear exhaust duct 50 mm with ventilation flap and front ventilation slide Connections BD series has RS422 interface for communication software switchable to printer output with RS 232 RS422 interface BF series nominal voltage 230 V Calibrations and validations possible please enquire Supplied with Binder test certificate Models with natural convection Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No BD 23 20 433x516x492 222x277x330 1 390 6146 BD 53 E2 53 634x575x617 400x330x400 1 390 6037 BD 115 E2 115 834x645x702 600x400x480 1 390 6039 BD 240 E2 240 1034x745x822 800x500x600 1 390 6041 BD 400 E2 400 1234x765x1022 1000x50
169. ral 45 topo 100 400 1000 130x250 1 734 3011 bra o lateral 45 topo 100 400 3000 178x341 1 734 3012 bra o lateral 45 t 100 40 6000 258x404 aoe A 734 3013 bra o lateral 45 t E 8000 293x445 opo 100 400 4 734 3014 bra o lateral45 Cell Culture Flasks Double Side Arm Celstir with Heating Jacket Wheaton 392 45 Teflon and silicone lined top cap Double side arm Celstir flasks complete with water jacket allows precise temperature control of flask contents when operated with a recirculating water bath Hose connectors accept 6 mm tubing Borosilicate glass flask Teflon and glass impeller assembly Capacity ml xH mm Cap size Pk Cat No 25 Shag EP IUEISIE 4 734 3178 X arm 15 415 top 38 430 side 4x147 1 734 317 a arm 15 415 Stota 1125 mi eg 734 3180 x arm 33 430 top 51 400 side 250 100x182 1 734 3181 arm 33 430 top 100 400 500 130x195 1 734 3182 side arm 45 top 100 400 1000 150x260 op i 1 734 3183 side arm 45 VWR E53 www vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Microcarrier Spinner Flasks Magna Flex Wheaton Borosilicate glass These flasks feature a flex type bulb shaped impeller which rotates from a fixed position above liquid level around an indentation in the base of the flask Primarily used with microcarrier cultures this stirring system increases stirring efficiency and provides a gentle even circu
170. rature Short SRK Swabs Rayon tip attached to the tube cap Each box contains 50 screw cap tubes Swab applicators are attached to the tube caps Description Packed Pk Cat No inse Solution 10 m ingle wrapped in plastic peel pouci l SRK Rinse Solution 10 ml 50 tubes box 50 710 0478 e Long SRK Swabs 14 6 cm Separate from cap Each box contains 25 pouches each containing a tube and applicator Swab applicators are separate from cap Description Packed Pk Cat No SRK Rinse Solution rayon 10 ml Tube and applicator in plastic peel pouch 25 710 0472 SRK Rinse Solution alginate 10 ml Tube and applicator in plastic peel pouch 25 710 0473 SRK Rinse Solution alginate 2 5 ml Tube and applicator in plastic peel pouch 25 710 0476 SRK Rinse Solution rayon 2 5ml Tube and applicator in plastic peel pouch 25 10 0431 gt Tel 0800 22 33 44 freephone Email uksales uk vwr com 300 14 Fax 01455 55 85 86 Web http uk vwr com Sampling Bags and Swabs Large SRK Swabs 19 50 cm long Separate from cap Each box contains 25 pouches each containing a tube and applicator Swab applicators are separate from cap Description Packed Pk Cat No SRK Rinse Solution Fiber tip rayon 10 ml Tube and applicator in plastic peel pouch 25 710 0474 Tube large applicator amp sterile 10x10 cm SSK Square Sampling Kit Fiber tip rayon 10 ml 1 Kit 710 0475 template mask in steril
171. reephone Email uksales uk vwr com 390 13 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Incubators Accessories Extensive range of accessories and factory fitted options such as interior programmable fluorescent uv lighting inner perspex door manual high low temperature alarms audible and visible with safety cut out built in chart recorder temperature programmers temperature controllers RS485 interface and access ports are available on request please enquire for further details Please note if application involves Drosophila a modification for aggressive environments is required please contact us before placing your order Fully programmable models are available Shelves supplied Type Inner volume I WxDxH ext mm WxDxH int mm max Pk Cat No 600W 600 690x800x2000 540x670x1460 5 24 1 390 0099 600WA 600 690x800x2000 540x670x1460 5 24 1 390 0168 1200W 1200 1370x800x2000 1240x670x1460 10 48 1 390 0083 A denotes dual temperature cycling models Please note that with the maximum number of shelves fitted in a cabinet there is only a 20 mm gap between each shelf Cooled Incubators Series 4 Programmable IMS The programmable option for LMS Series 4 cooled incubators is designed to offer the highest functionality and enables the user to set up and run complex processes relying on accurate temperature control The programmer function is able to control applications needing set point changes ov
172. rning CelIBIND Surface increases surface wetability for more even and consistent cell attachment Ultra Low Attachment flasks feature a covalently bound hydrogel layer that minimises cell attachment protein absorption and cellular activation e Treated for optimal cell attachment e Printed with lot numbers for ease in traceability e Sterilised by gamma radiation e Certified non pyrogenic 25 cm growth area flasks Description Neck Cap Pk Cat No Rectangular TC treated canted plug seal 500 734 1700 Rectangular TC treated canted phenolic 500 734 1706 Rectangular TC treated canted vented 200 734 1712 e Triangular TC treated angled phenolic 500 734 1531 Triangular TC treated angled vented 200 734 1532 Rectangular CellBIND surface canted vented 200 734 0090 Rectangular Ultra Low Attachment canted vented 24 734 4140 75 cm growth area flasks Description Neck Cap Pk Cat No Rectangular TC treated canted plug seal 100 734 1715 Rectangular TC treated canted phenolic 100 734 1716 Rectangular TC treated canted vented 100 734 1713 e Modified triangular straight phenolic 100 734 1543 Modified triangular straight vented 100 734 1544 Rectangular CellBIND surface canted vented 100 734 0091 Rectangular Ultra Low Attachment canted vented 24 734 4139 92 6 cm growth area RoboFlask vessels Description Cap Pk Cat No Cell culture vessel for manual
173. rom D D L L polylactic acid This material has a faceted architecture which is effective for culturing high density cell suspensions Applications include short and long term growth and differentiation of a variety of cell types including epithelial cells e g hepatocytes neurons endothelial cells osteoblasts chondrocytes fibroblasts and smooth muscle cells ECM pre incubation for specific stem progenitor cell differentiation assays dynamic cell seeding in larger vessels e g BD Falcon 50 ml conical tubes and non invasive evaluation of cell growth using the BD Oxygen Biosensor System e Support short and long term growth and differentiation of a variety of cells e Suitable for in vitro and in vivo basic research Description Pk Cat No BD 3D OPLA Scaffold 1 734 1062 BD Dispase BD Biosciences Dispase is a bacillus derived neutral metalloprotease that is recommended for recovering cell cultured on BD Matrigel Basement Membrane Matrix Dispase will yield a single cell suspension far more gently and effectively than trypsin collagenase or other proteolytic enzymes it will not harm cells harvested for sub cultivation or bioassays In addition Dispase may be used for tissue dissociation Dispase cleaves fibronectin collagen IV and to a lesser extent collagen but is does not cleave collagen V or laminin Description Pk Cat No BD Dispase 5000 caseinolytic units 100ml 734 1312 BD Cell R
174. s Description for volume ml Sterile Pk Cat No 20 300 2012 HDPE 500 mm 100 20 300 2013 PP 500 mm 100 20 300 2015 2 201 HDPE 1000 mm 190 9 at 2008 20 300 2011 PP 1000 mm 190 20 300 2014 Contact our customer service team Contact us by phone 0800 22 33 44 300 1 vwReE 5 by fax 01455 55 85 86 www vwr com Sampler StickProof Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Sampling Samplers Solid Made of stainless steel Sampler for powders in the pharmaceutical industry The conical inlet of the tip lets you collect a variable sample size StickProof is constructed in one piece without edges grooves or crevices for perfect and easy cleaning The sample can be filled directly into the sampling bag and fastened with a sturdy clamp StickProof Length 410 mm 25 mm boring 20 mm length of the chamber 120 mm sampling bags 170x120 mm e The especially slender tip can be inserted easily and deeply e Surface electropolished e Variable sample size of up to 50 ml Delivery Information Complete with clamp and 100 sampling bags PE Description Pk Cat No StickProof 1 312 1176 g Made of stainless steel the SiloPicker takes samples of bulk goods The insert depth depends on the density of the bulk goods Standard length is 1 m Inserting the SiloPicker into the bulk goods automatically closes the collection container When the sampling depth has been reached the sampling con
175. s TC treated individually wrapped 100 734 1607 48 Well plates Corning CellBIND surface 50 734 4067 392 9 VWR F www vwr com MicroWell Plates and MiniTrays Nunclon A Nunc PS high flange design sterile Ideal for cell culture cloning viral titrations and cell fusion 1934 as support Colour Clear e Plate edges and lids designed to reduce evaporation e Raised well rims reduce risk of cross contamination e Lids shaped to facilitate stacking and handling e 96 well footprint fits standard equipment e Plates tolerate low speed centrifugation with spacer plate Cat No 391 Cell Culture Cell Culture Plates and Dishes External LxW MicroWell plates 128x86 mm MicroWell MiniTrays 84x59 mm Recommended working Description volume ul Pk Cat No 96 MicroWell plate flat bottomed wells with lid a 1 ooo f 200 50 732 2607 96 MicroWell plate flat bottomed wells without lid 200 180 732 2601 96 MicroWell plate round bottomed wells with lid 200 50 734 2080 e 96 MicroWell plate round bottomed wells without lid 200 50 734 2033 60 MicroWell MiniTray conical wells with lid 8 150 734 2079 72 MicroWell MiniTray conical wells with lid 8 150 734 2005 Multidishes Nunclon A Nunc PS sterile with lid Useful in all areas of cell culture including scale up and cloning Recommended working e Raised well rims l
176. s only a 20 mm gap between each shelf 390 12 VWR 3 j www vwr com Cell Culture Incubators Cooled Incubators Series 3 Programmable EMS The programmable option for LMS Series 3 cooled incubators is designed to offer the highest functionality and enables the user to set up and run complex processes relying on accurate temperature control The programmer function is able to control applications needing set point changes over time e g ramp changes where a gradual rate of change can be set or step changes which are instantaneous These can be separated by soak periods during which the process is held at a constant value Each individual time interval of the program or segment together with its associated moving set point value can be stored as a unique program Via use of event outputs control of interior illumination and or alarm function is also available At the end of a sequence a program can be arranged to repeat or loop either a specified number of cycles or continuously For safety reasons three modes of recovery from power failure are available These either automatically re start the program from the beginning continue it from where it stopped or hold it waiting for a user re start Finally chart recorder data logging and configuration software for windows is available as optional extras This software is designed to operate to program all functions of the controller and to data log the recorded process signals including
177. se chamber slides have a chemically coated growth surface on the glass slide which mimics polylysine providing binding sites optimal for fastidious cells such as neurons E Description volume ml Growth area cm Pk Cat No 1 Chamber configuration 2 0 4 5 8 6 16 734 2051 2 Chamber configuration 1 0 2 0 4 0 16 734 2052 4 Chamber configuration 0 5 1 0 1 7 16 734 2053 8 Chamber configuration 0 2 0 5 0 7 16 734 2054 G Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com Web http uk vwr com 392 26 Cell Culture Cell Culture Chamber Slides Chambered Cover Glasses Lab Tek Nunc 1 0 Borosilicate coverglass PS medium chamber sterile These chamber slides are designed for confocal image analysis e Optimal for high power inverted microscopic viewing e Medium chamber is not removable Recommended working Description volume ml Growth area cm Pk Cat No 1 Chamber 2 5 4 5 9 4 16 734 2056 e 2 Chamber 1 2 2 0 4 2 16 734 2058 amber o Q 7 5 e 8 Chamber 0 2 0 4 0 8 16 734 2062 e Chambered Cover Glasses Lab Tek Il Nunc 1 5 Borosilicate coverglass PS medium chamber sterile For use with 1 2 4 and 8 well configuration Lab Tek II Chamber Slides e Excellent for confocal image analysis e Optimal for high power inverted microscope viewing e Chambered coverglass medium chamber is not removable Recommended working
178. stradiol 17 amp testosterone hydrocortisone selenous acid o phosphorylethanolamine glucose amino acids vitamins and other trace elements and nutrients contained in the Ham s F12 medium base Tested at a concentration of 10 for the ability to stimulate a 100 fold increase in growth of BHK 21 and a 13 fold increase in growth of BALB c 3T3 cells Tested for total protein content pH and osmolarity Quality control Filtered 0 2 um membrane and tested and found negative for bacteria fungi mycoplasma and viruses bovine diarrhea virus bovine parvovirus bovine adenovirus and rabies virus also tested for the absence of cytopathic effects CPE inclusion bodies and haemadsorption Tested for endotoxin LAL assay Formulation Recommended concentration Replaces foetal bovine and other sera on an equivalent volume basis Storage and stability Stable for at least three months at 20 C Description Pk Cat No BD Nu Serum 500 ml 734 1318 BD Collagen Il BD Biosciences Collagen II is the principal collagenous component of cartilage intervertebral disc and vitreous humour Collagen II supports chondrocyte adhesion and may influence the differentiated phenotype of these cells In culture Collagen II is used for attachment and differentiation of chondrocytes It can also be used as an in vivo model in rats and mice for arthritis studies injection of Bovine Collagen II induces arthritis
179. tainer is filled and the SiloPicker can be withdrawn e Easy handling e With the extension rod it is possible to take samples of bulk goods at a depth of up to 3 5 m Description Pk Cat No SiloPicker 1 300 1025 Accessories Description Pk Cat No Extension rod 50 cm 1 300 1026 Extension rod 100 cm 1 300 1037 For sampling bulk goods of all types The big advantage of the zone sampler is that cross sectional samples can be collected from all layers of a container Using the zone sampler very different kinds of bulk goods can be collected from the finest powder up to coarse grains such as corn or nuts The zone samplers are available in 3 lengths 55 cm for sacks 85 cm for barrels drums and mixing containers and 150 cm for bags silos and tanks Special lengths are available on request Three types of zone samplers are available Multi sampler with several closed chambers for multi target samples from several different sample depths Uno sampler with one closed chamber for a targeted sample from a specific sample depth All layer sampler and Jumbo sampler with open inner tube no chambers for cross sectional samples throughout all layers of sampling material Emptying is simple via the open end of the sampler tube The Jumbo sampler with a 50 mm tube diameter is ideal for larger grain sizes and quantities All samplers have the smallest possible gap between inner and outer tube e Easy to clean remova
180. tainless steel for B 6 und UB 6 1 390 1331 Microbiological Incubators Heraeus Series 6000 Thermo Scientific i Temperature range ambient 5 to 70 C volumes 30 to 750 I Extensive range of standard equipment with uniquely modular design that allows them to be configured to meet specific individual needs Optimal homogeneous and stable environment provides temperature uniformity and stability ensuring fully reproducible high quality results Larger models are available with forced air circulation UB for superior temperature homogeneity Door seals are made of silicone and can be easily replaced Left hinged and lockable doors available on request Other accessories available on request Cin Sa e Electronically regulated temperature PID action e Protection from overheating with separate sensor e Short heating up times and high temperature stability e Minimal temperature overshoot Design Robust exterior made of galvanised pre coated sheet steel Stainless steel incubator chamber and cover plates Stainless steel interior heater Modular operating and control unit With damper to control extracted air as standard Right hinged doors as standard Cleaning Corrosion resistant stainless steel chamber with rounded corners and interior fixtures easy to clean Control Temporal temperature stability of 0 5 C according to DIN 58945 electronically controlled with PID action Kelvitron and non contacting switc
181. th area of 50 cm2 total 100 cm2 with 75 um thick membranes 2 mm apart Applications include transportation of live cells hybridoma antibody production OptiCell MAX short term freezing and thawing of cells biomagnetic cell separation OptiMag cell imaging and staining and transfection e Thin profile design maximises incubator space and reduces medium consumption recommended medium cell fill volume 10 ml for OptiCell and 30 ml for OptiCell MAX e Two resealing access ports provide closed growth environment with sterile fluid path thereby reducing risk of contamination e Barcoded for easy tracking and automatic handling OptiCell is a registered trademark of BioCrystal LTD Description Pk Cat No OptiCell 1100 starter kit containing 20 OptiCell 1100 chambers 1 rack 1 knife 50 tips 2 caps 1 p 734 2221 p optical shield manual 1 mini CD 20 734 2222 OptiCell 1100 chambers 100 734 2223 500 734 2224 OptiCell MAX kits and chambers Description Pk Cat No ptice starter kit containing ptice chambers 1 rack 50 tips hybridoma 1 734 2225 antibody production protocol manual OptiCell MAX 2100 chambers 20 eau i p 100 734 2227 392 7 VWR E F www vwr com Cell Culture Cell Culture Flasks OptiCell separation kit and magnet Description Pk Cat No OptiMag cell separation kit containing 20 OptiCell 1100 chambers 1 magnet 1 rack 50 tips 2 clips 4 392 0600 OptiMag cell separation
182. the back or side wall of the chamber for fan assisted units 200 litre only The top vent is fitted with a clip to hold a mercury in glass thermometer Shelves All units have fixed shelf runners and chrome plated wire grid shelves MINI 6 has one shelf MINI 30 INC 50 and INC 75 units are supplied with 2 shelves INC 100 INC 125 and INC 150 have 3 INC 200 has 4 shelves Control The control system comprises a Microprocessor digital PID controller with dual displays of set point and actual temperature The control system comprises a direct reading thermostat and over temperature thermostat both with calibrated scales and tamper proof locks They also include main switch with indicator and heat and overheat indicators Accessories A wide range of options and accessories is available including models with mild steel coated with aluminium CLAD chambers fan circulation and doors with integral viewing window High capacity units up to 1250 litres are also available please enquire for details Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No MINI 6 6 320x330x390 230x190x150 1 390 0337 MINI 30 30 480x490x500 360x350x240 1 390 0336 INC 50 50 730x470x570 410x340x420 1 390 0356 INC 75 75 850 470x570 530x340x420 1 390 0357 INC 100 100 850x590x570 530x460x420 1 390 0346 INC 125 125 850x590x680 530x460x520 1 390 0347 INC 150 150 850x660x680 530x540x520 1 390 0348 INC 200 200 850x660x870 490x540x750 1 390 0349
183. tion and contamination due to improper lid fit is eliminated In the Feeding Position pipette access is improved for fluid handling on the basolateral side In the Incubation Position cell culture inserts remain locked in position in their companion plate wells e Reagents can be added quickly and consistently for timed experiments e Aspiration from the well is easier reducing the risk of contamination e Media cannot wick up between the insert and the well wall e Low evaporation lid reduces evaporation and contamination Note May be used with or without cell culture inserts Description Pk Cat No 6 Well companion plate deep well 4 734 1095 6 Well companion plate 50 734 0065 12 Well companion plate 50 734 0066 24 Well companion plate 50 734 0067 BD BioCoat Matrigel Invasion Chambers BD Biosciences An in vitro system for assessing the invasive potential of both malignant and normal cells BD BioCoat Matrigel Invasion Chambers enable studies of metastatic potential of tumour cells expression of matrix metalloproteinase on the surface of invasive tumour cells inhibition of metastasis by ECM components or antineoplastic drugs i e Taxol altered expression of cell surface proteins in metastatic cells and invasion of normal cells such as embryonic stem cells cytotrophoblasts and fibroblasts as well as of multiple cell lines The BD BioCoat Matrigel Invasion Chamber is an in v
184. to 1 program with 20 sections integrated weekly program timer elapsed time indicator adjustable fan speed and electronical controlled APT line preaheating chamber Connections RS422 interface for APT COM standard communication software nominal voltage 230 V 200 240 V KBW 240 Calibrations and validations possible Supplied with Binder test certificate Temp Inner Type range C Volume I volume I WxDxH ext mm WxDxH int mm Pk Cat No KBW 240 0 70 240 240 925x800x1460 650x485x785 1 390 0330 KBW 400 0 70 400 400 884x716x1850 650x470x1308 1 390 6078 KBW 720 0 70 720 720 1250x887x1925 973x576x1250 1 390 0359 Humidity Chambers HCP Range Memmert Humidity chambers which have corrosion resistant stainless steel inner chamber and housing for long term climatic tests at constant temperature Ei and humidity Ideal for food processing cosmetics pharmaceutical biotechnology and electronics industries Units feature an auto diagnostic system with fault indication for temperature and humidity control The working chamber is heated on all six sides this together with electronic humidity control prevents condensation forming in the chamber Distilled water is supplied from an external 3 litre tank by means of a self priming pump GLP GMP compliance is assisted by the serial USB interface and Celsius software for programming and documentation e Multifunctional PERFECT PID microprocessor controlle
185. to the highest classification standard for medical devices class lla surgical type device for temporary one time use For collecting samples from the natural orifices of the body and surgical scars Available with a range of different applicators and transport media e Applicator and tube ensure viability of a wide range of bacteria for 24 to 48 hours e Performance tested for an enormous range of aerobic and anaerobic organisms e Each swab is packaged individually in a bag with a tamper proof closure which turns white after opening Description Pk Cat No Wood cotton tipped Amies gel 50 710 0424 Wood cotton tipped Amies gel with charcoal 50 710 0425 Wood cotton tipped Stuart gel with charcoal 50 710 0426 Plastic viscose Stuart gel 50 710 0443 e Wood cotton tipped Stuart gel 50 710 0444 Aluminium viscose Stuart gel 50 710 0445 e Plastic viscose Stuart gel with charcoal 50 710 0446 Plastic alginate Amies gel 50 710 0447 Plastic viscose Cary Blair gel 50 710 0449 e e business more than just a webshop For all of your laboratory needs Please visit us at http eu vwr com e business 300 13 VW R E F www vwr com Sampling Bags and Swabs Swabs Simple swabs in tubes High quality polypropylene suitable for medical uses guarantees that all swab tubes are non toxic and have low moisture permeability e Sterile e Each tube has a tamper proof cap e The batch number exp
186. ture distribution throughout the chamber e Units have an intelligent switch built into the digital controller that automatically switches the cooling compressor off at a set point and when temperatures above ambient are required to conserve energy e User can leave the unit unattended and can be confident that the minimum amount of energy is being used for the incubator performance Design Exterior is mild steel finished in white enamel paint Durable easy to clean moulded plastic inner chamber fitted with a stainless steel back plate Shelves All units are fitted with integral shelf runners and removable plastic coated wire grid shelves Cl 50 is supplied with 2 shelves Cl 100 has 3 Cl 200 has 6 and Cl 250 has 7 shelves Control The control system comprises a microprocessor digital PID controller with dual displays of set point and actual temperature Tel 0800 22 33 44 freephone Email uksales uk vwr com 390 11 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Incubators Accessories A wide range of options and accessories is available including timers fully programmable controllers communication and access ports interior lights internal 13 amp sockets viewing window audible and visual alarms and chart recorders Please enquire for details Type Inner volume I WxDxH ext mm WxDxH int mm Pk Cat No CI 50 50 500x550x750 420x300x470 1 390 0335 C1 100 100 500x630x950 420x380x680 1 390 0332
187. um culture studies of the effects of collagen on cell behavior improving survival of primary cell lines in culture and cell adhesion assays e Uniform application of rat tail collagen type for consistent performance e Manufactured in a highly controlled environment and rigorously tested to assure product consistency and performance Description Colour Pk Cat No 6 Well ol transparent 5 734 0108 Well piate transparent 50 734 0274 12 Well plate transparent 50 734 0295 transparent 5 734 0115 24 Well plate transparent 50 734 0277 48 Well plate transparent 50 734 0296 transparent e transparent 50 734 0276 96 Well plate black clear 50 734 0319 white clear 50 734 0320 white 50 734 0303 black clear 50 734 0332 hite cl 50 734 0329 384 Well plate lesa transparent 50 734 0331 white 50 734 0330 BD BioCoat Poly L Lysine and Poly D Lysine Cell Culture Plates BD Biosciences PS coated with Poly L Lysine or Poly D Lysine sterile non pyrogenic Poly D Lysine PDL and Poly L Lysine PLL are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate Poly L Lysine and Poly D Lysine surface treatments support applications including attachment and spreading of a variety of cell lines cell differentiation and neurite outgrowth attachment of transfected cell lines and survival of primary neurons in culture As PDL
188. und coverslips 13 mm for 24 well multidish 500 734 2117 g Round coverslips 15 mm 500 734 2172 e Round coverslips 22 mm 500 734 2173 Round coverslips 25 mm for 6 well multidish 500 734 2118 Microscope Slides LiveCell Array Nunc transparent array of picowells Disposable sterile microscope slides with an embedded LiveCell Array is a slide based tool for real time study of individual living cells adhering and non adhering within heterogeneous populations e Enables multiple functional assays on living cells followed by post fixation studies on the same cells e Staining rinsing and perfusion do not displace cells e Compatible with standard microscopes e Image Analysis Software designates and addresses to each cell LiveCell is a licensed trademark of Molecular Cytomics Inc LiveCell Array Slides 1 per case Description Pk Cat No Slide with 15 um well 1 734 2198 Slide with 20 um well 1 734 2200 Slide with 100 um well 1 392 0010 Slide with 250 um well 1 734 2202 LiveCell Array Slides 5 per case Description Pk Cat No Slide with 15 um well 5 734 2199 Slide with 20 um well 5 392 0008 Slide with 100 um well 5 392 0009 Slide with 250 um well 5 734 2203 LiveCell Array Apoptosis Kits 5 slides plus reagents Description Pk Cat No Slide with 15 um well 5 734 2204 Slide with 20 um well 5 734 2205 Slide with 100 um well 5 734 2206 Slid
189. und heating and double doors glass inner door stainless steel outer door The fresh air supply can be adjusted manually via the pre heating chamber electronically regulated on the PERFECT model with air valve controller The units are equipped with dual over temperature protection multiple protection and class 3 1 on PERFECT models The BASIC model e Integral digital timer from 1 min to 99 hr 59 min e LED display of setpoint and actual temperatures and remaining programme time e PID controller EXCELLENT and PERFECT models e Real time weekly programme timer ramp timer for 40 variable ramps each from 1 min to 999 hr e Long term logging of all relevant data via 1024 kB ring buffer e RS232 serial interface with Celsius programming and logging software e Adaptation of effective heating power depending on setpoint e 2 High grade platinum temperature sensors PT 100 in a 4 wire circuit for stable long term transmission of measurement signals The PERFECT model also features e Printer interface e 32 kB MEMory Card for programming and documenting up to 40 ramps e STERI Card with fixed programme for inner chamber sterilisation at 160 C for 4 hours e Manufacturers calibration certificate at 37 C e Visualisation of all process functions on the display with additional language selection via set up for alphanumeric text display Shelves Inner supplied Type volume I WxDxH ext
190. ure Innoculating Needles and Accessories Spreaders T shaped VWR gt Plastic sterile w Designed for spreading and dispersing liquids across the surface of agar culture plates Used for performing bacterial CFU plate counts on water milk and other liquid samples Smooth rounded surfaces to prevent cutting or gouging of agar e T Shaped to ensure even pressure is applied while spreading Description Colour Packed Pk Cat No blue 1 peel pouch 500 612 2651 T Shaped spreader blue 5 peel pouch 1000 612 2652 ue peel pouc g Cotton Wool e Absorbent Description Pk Cat No Cotton wool hospital quality 1 Roll 118 0300 Cotton woo BPC British Pharmacy Code 500 g 118 0301 Cotton Wool e Available in choice of colours e Non absorbent Description Pk Cat No Cotton wool yellow 500 g 391 2263 Cotton wool white 500 g 391 2264 G BugStopper Whatman that provides reliable sterile ventilation for culture containers The cap is made of biologically inert silicone The ventilation device consists of an ultra fine glass microfibre filter which is reinforced with a coating of polyester monofilament The ventilation device is surrounded by a reinforcement ring made of stainless steel For bacterial viral and cell cultures BugStopper is a unique reusable cap e For preserving sample integrity even during use autoclavable e The BugStopper can be punctured with
191. use TC treated with bar code septum 100 734 4045 Cell culture vessel for manual use TC treated with bar code septum 50 734 4044 Cell culture vessel for manual use TC treated with bar code flat without septum 100 734 4046 Cell culture vessel for manual use TC treated with bar code flat without septum 50 734 4041 Cell culture vessel for automation CellBIND surface with bar code septum 100 734 4042 Cell culture vessel for automation CellIBIND surface with bar code septum 50 734 4043 100 cm growth area low profile flasks Description Cap Pk Cat No Low profile CellBIND surface vented 60 734 4047 Low profile TC treated vented 60 734 4141 Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com Web http uk vwr com 392 4 Cell Culture Cell Culture Flasks 150 cm growth area flasks Description Neck Cap Pk Cat No Rectangular TC treated canted plug seal 50 734 1717 Rectangular TC treated canted phenolic 50 734 1718 Rectangular TC treated canted vented 50 734 1719 Rectangular CellBIND surface canted vented 50 734 0092 162 cm growth area flasks Description Neck Cap Pk Cat No Traditional TC treated straight phenolic 25 734 1540 Traditional TC treated straight phenolic 25 734 1541 5 175 cm growth area flasks Description Neck Cap Pk Cat No Rectangular TC treated angled plug seal 50 734 1722 Rectangular TC treated ang
192. utoclavable Description Pk Cat No Culture vessel 12 1 734 5055 Culture vessel system with BioTech mixer 220V 1 734 5056 Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 44 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Cell Culture Flasks Double Side Arm Celstir Wheaton Teflon and silicone lined top cap Double side arm Celstir flasks are ideal for microcarrier and suspension cultures such as insect cells hybridomas and adapted cell lines Borosilicate glass flask Teflon and glass impeller assembly e Adjustable paddle blade impeller allows better mixing e Impeller does not protrude through the top cap thereby maximising incubator space and reducing the risk of contamination e Addition of the bottom dimple to flasks 125 ml and larger improves circulation and reduces the accumulation of cells in the centre of the flask e Flasks 500 ml or larger have a 45 mm side arm to be used as an air vent media inlet or outlet innoculation port pH probe inlet or other application Volume ml xH mm Cap size Pk Cat No topo 38 430 25 38x122 bra o lateral 15 1 734 3006 415 topo 38 430 50 38x141 bra o lateral 15 1 734 3007 415 topo 51 400 125 65x155 bra o lateral 33 1 734 3008 430 topo 51 400 250 85x175 bra o lateral 33 1 734 3009 430 Sah t E 500 110x190 opo 100 400 4 734 3010 bra o late
193. vailability of these standardised assays has facilitated a better understanding of the molecular mechanism of angiogenesis and simplified the routine use of cell based assays for screening of anti and pro angiogenic compounds BD Human Umbilical Vein Endothelial Cells HUVEC 2 are derived from single donors and cryopreserved at passage number 2 HUVEC 2 cells have been pre qualified to assure a robust migratory response to angiogenic factors such as VEGF and FBS Single donor primary HUVEC 2 cells are suitable for use in combination with BD BioCoat Angiogenesis Assay Systems to provide relevant models for angiogenesis e g cardiovascular vascular and wound healing and cancer research BD Human Umbilical Vein Endothelial Cells have been qualified for use in BD BioCoat Angiogenesis Endothelial Cell Migration assays and may be used in BD BioCoat Endothelial Cell Invasion and Tube Formation assays e Address key steps in the angiogenesis process using standardised cell based assays Increase throughput with screening compatible formats and data acquisition options e Obtain human umbilical vein endothelial cells pre qualified for use in the endothelial cell migration assay 392 31 VWR 3 j www vwr com Cell Culture Cell Culture Inserts All BD BioCoat Angiogenesis Systems are tested for the ability to support HUVEC tubule formation determined by tubule length and measured by automated image analysis All lots of this
194. ven e Accepts bottles from 108 to 121 mm in diameter and up to 550 mm in length e Locking speed control knob prevents accidental change in rotation rates e Supplied without vessels available separately Power 230 V 50 60 Hz 35 W Bottle speed 0 25 to 5 3 rpm WxDxH Base unit with 1 deck 755x622x340 mm each additional deck 180 mm high Weight Base unit with 1 deck 32 kg each additional deck an additional 9 5 kg Warning Not to be used in temperatures exceeding 40 C Description Pk Cat No Base with 1 deck 5 bottle positions UK plug 1 734 3064 Base with 2 decks 10 bottle positions UK plug 1 734 3067 Base with 3 decks 15 bottle positions UK plug 1 734 3070 Base with 4 decks 20 bottle positions UK plug 1 734 3073 Base with 5 decks 25 bottle positions UK plug 1 734 3076 Base with 6 decks 30 bottle positions UK plug 1 734 3079 Base with 7 decks 35 bottle positions UK plug 1 734 3082 Base with 8 decks 40 bottle positions UK plug 1 734 3085 Base with 9 decks 45 bottle positions UK plug 1 734 3088 Accessories Description Pk Cat No 5 position deck with all position drive 1 734 3062 allection Please contact your local VWR sabes office or go to wwwvwrcam for the latest news and special offers Tel 0800 22 33 44 freephone Email uksales uk vwr com 392 48 Fax 01455 55 85 86 Web http uk vwr com Cell Culture Cell Culture Larger Scale Bioprocessing Small Bottle Bench
195. white when opened Meets the highest level of classification for medical products Class lla surgical invasive short term application for taking samples from natural orifices and surgical wounds y Description Pk Cat No Swab plastic viscose Amies gel 50 710 0432 Swab aluminium viscose Amies gel 50 710 0433 Swab plastic viscose Amies gel with charcoal 50 710 0434 e Swab aluminium viscose Amies gel with charcoal 50 710 0435 e Swab twisted aluminium viscose Amies gel 50 710 0436 Swab twisted aluminium viscose Amies gel with charcoal 50 710 0437 e Swab plastic viscose Amies liquid 50 710 0438 Swab aluminium viscose Amies liquid 50 710 0439 Swab twisted aluminium viscose Amies liquid 50 710 0440 Swab plastic viscose Stuart liquid O D0 TOA Tel 0800 22 33 44 freephone Fax 01455 55 85 86 Email uksales uk vwr com Web http uk vwr com 300 12 Sampling Bags and Swabs Swab Systems M40 VWR es The M40 swab system with transport medium improves recovery and ensures the survival of even the most fastidious microorganisms e Compliant with NCCLS standards at room temperature and 4 C e Without charcoal Delivery Information Comprises plastic indicator rayon tip and Amies gel Description Pk Cat No M40 swab 50 710 0442 Swabs with Transport Medium A range of collection and transport devices for bacterial specimens Conform
196. y and soil samples soil sediments oils emulsions creams etc Colour coded handles are available in red blue green yellow white and black avoid confusion Easy cleaning All surfaces are poreless and without crevices Only round threads tried and tested in food hygiene are employed e Powerful suction for viscous media e No dirt accumulation e Dissembling and cleaning is very easy Description Pk Cat No ViscoSampler PTFE FEP 1m 1 331 2160 e ViscoSampler PP 1m 1 331 2162 Accessories Description Pk Cat No Tube cleaning brush 25 mm 1 m 1 331 2170 G Extension rod 1 331 2171 Email uksales uk vwr com Web http uk vwr com 300 6 Sampling Samplers Fluid Sampler with Hose and Hose Weight Stainless Steel UniSampler Dipping Bottle Ex Dipping Vessel 300 7 The heavy stainless steel tube brings the hose to any desired depth The suction hose is made of PVC and 2 50 m long Operation lower the suction hose into the medium attach the sampling bottle to the adapter create a vacuum using the handpump and collect the sample remove the filled sampling bottle close and label e Sampler for deep narrow and hard to reach spots The samples are collected using a handoperated vacuum pump and transferred directly into the sampling bottle to ensure sampling purity e Easy handling Delivery Information Supplied complete in case with sample bottle hose handpump and 20 labels
197. yl Films for Robotics vwRA J Description _ temporary protection of samples in 96 well plates from contamination and These 100 um thick vinyl films with 20 um adhesive layer are designed for evaporation A precut pattern over each well separates the film into four flaps that bend inward easily when pushed by a robotic probe or pipette tip allowing access to the sample without coring or adhesive fouling The resilient flaps regain their original position after sampling for continued sample protection For long term sample protection after sampling a continuous film should be applied as a second layer Suitable for sealing all standard 96 well plates e Protect samples and limit evaporation short term e Precut flaps bend inward without fouling probes or tips e Flaps close for continued protection after sampling Each film LxD 145 5x79 4 mm Pk Cat No Precut vinyl films non sterile 100 391 1286 Precut vinyl films sterile 50 391 1287 Breathable Sealing Film Axygen Description Porous film seals PP and PS culture plates 96 and 384 well plates and other assay plates e Allows effective gas exchange for cellular and bacterial cultivation and prevents contamination e Sterile packs available Pk Cat No Breathable film non sterile 100 732 1027 Breathable film sterile 50 732 1028 392 57 VWR E53 www vwr com Cell Culture Cell Culture Accessories and Seali

Sampling and Sample Culture

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