one shot cell disrupter
Contents
1. Section C Glossary TONS Section D Partsof the Machine uuu uu u u u uuu etis Section E joo Section F Section G I cle e HE Q Section H Maleae uuu u O Section J Troubleshooting S Section K P Section L Declaration of Confalfniby uiii uuu Section M Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 K TROUBLESHOOTING 1 No power e g digital panel meter does not illuminate and power on light not showing Not plugged in Not switched on Fuse faulty in plug or circuit breaker out Trip on front of panel DPM faulty DPM Digital Panel Meter Faulty power on LED Digital display does not illuminate Check power is applied to machine 24V DC power supply failed Contact service agent Pressure control knob does NOT change digital display Knob loose on spindle Control potentiometer faulty 24V DC power supply faulty Display faulty Contact Service Agent Pressure cycle not completed within 50 seconds of machine being started Solenoid failure contact Service Agent Solenoid valve failure contact Service Agent Blocked jet increase pre2. Section E joo za M Section F Section G IP ge uuu Section H Maihtena E Mm Section J uu u u U Section K Spec MEIO Me H N Section Declaration of Confalfniby u u uu uuu u uuu uuu Section M Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 Cup Lip TARGET e Jer UVES CU cca Section A Safety Section luii Ln e Section C Glossary TONS Section D Main Parts of the Machine uuu uu u uuu u uuu u u u uuu suma Section E Section F Pici Section G uyu Section H Maihtena E Mm Section J uu u u U Section K Spec MEIO Me H N Section Declaration of Confalfniby u u uu uuu u uuu uuu Section M Log Book Constant Systems Limited Low March Da
3. Section D Partsof the Machine uuu uu u u u uuu etis Section E joo Section F Section G DtaWwinGS aa Section H Tm Section J u u u u u u uu u uu Section K SSDS CHI CAIN sce set Section L Declaration of Confalfniby u ees nae Section M Log Book Constant Systems Limited Low March Daventry Northants NN11 450 England Tel 44 0 132 Fax 44 0 1327 314 147 CONSTANT 122 Cons TANT ae 44 Cell Disrupter Installation Operation amp Maintenance Man Ew ual SysTEMS f J MAINTENANCE the following maintenance procedures are routinely carried out at the recommended 6 monthly service of the machine Additionally any of them can be carried out on site for cleaning and decontamination purposes for example when changing samples Removal and Replacement of the HP Seal To remove the HP Seal it is necessary first to remove the cylinder To Remove the Cylinder f 2 Ensure the probe is not in the machine Remove the At the end of the last disruption cycle the piston will have returned to the bottom of its travel Press SLOW UP and the piston will lift the cylinder up to the top of its travel This is illustrated at DRG 3D 1244 In
4. 2 le gt CONSTANT One Shot Cell Disrupters Operator s Manual SYSTEMS CONSTANT SYSTEMS LIMITED OPERATOR S MANUAL For ONE SHOT CELL DISRUPTER This Operating Manual Contains Important Safety Information Read Thoroughly Before First Use Make Available To All Machine Users Constant Systems Ltd Low March Daventry Northants NN11 4SD United Kingdom Tel 44 0 1327 314146 Fax 44 0 1327 314147 E mail constant score group com Website www constantsystems com CONSTANT SYSTEMS LTD HAS TAKEN EVERY CARE TO ENSURE THAT THE INFORMATION CONTAINED WITHIN THIS MANUAL IS FACTUAL ACCURATE AND COMPLIANT WITH STANDARD BS EN 61010 2001 HOWEVER THEY CANNOT BE HELD LIABLE FOR ANY ERRORS OR OMISSIONS INFORMATION IN THIS DOCUMENT IS SUBJECT TO CHANGE WITHOUT NOTICE AND DOES NOT REPRESENT A COMMITMENT ON THE PART OF CONSTANT SYSTEMS LTD E Copyright Constant Systems Limited Title Page 1 of 1 DELIVERY METHOD STATEMENT FOR CONSTANT SYSTEMS LIMITED CSL MACHINES EXTREME CARE SHOULD BE TAKEN WHEN HANDLING OR MOVING CELL DISRUPTER MACHINES TO AVOID PERSONAL INJURY THE CELL DISRUPTER WILL BE PALLETISED AND DELIVERED BY COURIER FOR INSTALLATION THE CLIENT IS RESPONSIBLE FOR THE UNLOADING OF PALLETISED MACHINES AND FOR TRANSPORTING THE PALLETISED MACHINE TO THE POINT OF USE AT THE POINT OF USE PALLETISED MACHINES SHOULD BE UNPACKED AND THE MACHINE TRAN
5. Section L Section M IA Cell Disrupter Installation Operation amp Maintenance Manual LT T M Systems f D GLOSSARY OF TERMS Aerosol That part of the disrupted product that is in the form a gas The process of cleaning sterilising by application of compressed Autoclaving S steam at a temperature of approximately 150 C The outer casing of the disruption assembly which must be in place Body 4 when disruption cycle is taking place Cup The container which collects the disrupted product Cycle See Disruption Cycle The term used in the industry to describe the remnant which is left Dead Loss unprocessed Disruption Cycle The process which begins when the probe is pressed and ends when the machine fires audibly Usually approximately 20 seconds Disruption Pressure The pressure at which the sample is forced through the jet This pressure persists for only a fraction of a second in each disruption cycle High pressure seal which forms the bottom of the cavity which holds peal the sample It is driven upwards during a disruption cycle by the piston A cylindrical cavity formed by the inside surface of the cylinder and p y the HP Seal at the bottom A sub assembly in which a sapphire with a drilled hole of 0 18mm Jet dia is mounted in a stainless steel holder The jet is fitted into the bottom of the cup Piston The
6. of Intertek Testing Services and is not transferable Only the Applicant may reproduce this document The Certification Mark s may be applied only at the above noted location of the Party Authorised to Apoly Mark c us d s r 7 47 4 Authorised by 7 Date 29 March 2007 Gun for wo ctt Contro Number 3092407 This Cocument supersedes ail previous Authonsations 10 Mark for te noted Report Number intenek Testing Services NA inc 165 Main Street Cortland 13045 Telephone 800 345 385 or 607 753 6711 Fax 607 756 6699 nm CONSTANT Cell Disrupter Installation Operation 4 Manual CONSTANT SYSTEMS CELL DISRUPTER LOG BOOK DATE VOLUME Copyright Constant Systems Limited Logbook 1 of 1
7. the piston shaft from turning using the open ended spanner and turn the arms of the T bar clockwise to tighten the HP Seal Remove the T bar and socket Remove the 14mm open ended spanner from the piston shaft Replace the two part retainer clip Reconnect or switch on the power supply Press SLOW DOWN until the PISTON DOWN lamp illuminates To Refit the Cylinder Press the cylinder down over the HP Seal carefully ensuring that no damage takes place to the seal Removal and Replacement of the Jet The jet consists of a 0 18mm dia hole drilled in sapphire which is mounted in stainless steel It does not wear out and becomes blocked only when solid matter for example glass shards or particles of plastic obstruct or partially obstruct the orifice by becoming lodged in it A blocked jet must be replaced with a new one The jet is an interference fit in the base of the stainless steel cup The procedure for Removal and Replacement of the Jet is illustrated on DRG 3D 1473 Copyright Constant Systems Limited _ SectionJ 2 of 3 CONSTANT 22 CONSTANT 5 2522 Cell Disrupter Installation Operation 4 NS SysTEms Lr f To Remove the Jet from the Cup 1 Place the Jet Removal Holder on a firm workbench 2 Place the cup on the holder so that the jet fits into the hole 3 Insert the Jet Removal Tool into the pipe in the cup and tap it with a nylon or bra
8. 0 1327 314 147 Section A Section B Section C Section D Section E Section F Section G Section H Section J Section K Section L Section M A INTRODUCTION What is Cell Disruption Cell Disrupter Systems are designed to selectively break the cell wall allowing intracellular components to be released and harvested This is particularly for use in Pharmaceutical and Biotechnology companies Universities research institutes and agencies Cell Disruption is achieved through the use of high pressure which accelerates a sample through a small fixed orifice at high velocity under controlled contained and repeatable conditions There are three stages to the effective cell disruption 1 The user manually loads the cell disrupter with the sample 2 The piston then compresses the sample at a constant pressure accelerating it through the small fixed orifice at high velocity The acceleration of the product through this fixed orifice causes cell disruption 3 When the jet containing disrupted cells hits the target it is spread radially then vertically down the cooled heat exchange surface of the cup Product is then contained within the cup for collection by the user Using the machine The One Shot cell disrupter will enable you to work with quantities of material between 1 and 20 5 mls depending on the maximum pressure ordered There is sometimes a tiny amount of material not processed on the first str
9. 8 Only if necessary Adjust the pressure using the knob on the front of the cabinet 9 Pipette an appropriate volume of the sample into the hole in the top of the cylinder Ensure that the chamber is not overfilled because this would prevent the cup and lid from fitting properly 10 Fitthe cup with lid on into the top of the cylinder to collect the output 11 Place the Body over the cylinder and lower it down to the surface on which it will rest Rotate it until it drops on to the surface and rotate it again to align the marks with each other The marks are illustrated on DRG 3D 1243 Sheet 1 in the callout This last step is necessary to be able to insert the probe 12 Insert the probe CAUTION At the next step ensure that the probe is held pressed until the PISTON DOWN lamp extinguishes AND ILLUMINATES AGAIN Failure to observe this caution will abort the disruption cycle prematurely 13 Press the probe downwards and hold it until you hear the machine fire not a loud noise the PISTON DOWN lamp extinguishes and re illuminates 14 Release and then remove the probe The piston will have automatically returned to its lowest point and the disruption cycle is complete 15 Remove the Body by turning it and lifting it clear of the cylinder 16 Remove the cup from the top of the cylinder using the Cup Lifting Tool 17 Remove the lid from the cup using the Cup Lid Lever The disrupted sample can now be removed form
10. Ip ge uuu Section H Maihtena E Mm Section J uu u u U Section K Spec MEIO Me H N Section Declaration of Confalfniby u u uu uuu u uuu uuu Section M Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 0 Systems f lt Cell Disrupter Installation Operation amp Maintenance Manual B SAFETY CONSIDERATIONS Various safety warning signs are attached to the machine You must consult this documentation to read about the hazard in question particularly where the exclamation mark in triangle appears CAUTION Be aware that power should be switched off when dismantling parts of the disruption head for maintenance cleaning Instruction to switch on the machine and later off again where this is necessary are included in the text CAUTION If any work which involves dismantling any of the disruption head is to be done on the machine the electrical supply must be isolated from the mains by the clients isolator WARNING If the machine is used in any way that is not described in this document the protection provided by the equipment may be impaired CAUTION The loading and unloading of product into and from the machine is the responsibility of the user and t
11. SFERRED TO THE WORKSTATION OR IF ORDERED THE OPTIONAL BOLT UP TROLLEY BY MEANS OF A MANUAL LIFT MACHINE WEIGHTS ARE AS FOLLOWS approx for info only and add 40kg for palletised Bench Top machines ONE SHOT MACHINE 85kg CRATE SIZES ARE AS FOLLOWS width x depth x height in mm approx for info only ONE SHOT MACHINE 850 x 760 x 1100 AFTER INSTALLATION THE CLIENT IS RESPONSIBLE FOR THE SAFE REMOVAL OF ALL PACKAGING MATERIALS AND DISPOSAL IN AN ENVIRONMENTALLY ACCEPTABLE MANNER Copyright Constant Systems Limited a CONSTANT AES SYSTEMS E f Dear Customer Thank you for selecting a Constant Systems Cell Disrupter We would appreciate feedback on the performance of this machine Please complete the following short survey document after at least two full months of operation or fill in an on line version at www constantsystems com 1 Please score the performance of the machine out of a maximum top score of 10 for the following categories a Ease of operation b Reliability Overall Performance 2 Please detail clearly any problems you have experienced are experiencing with the machine 3 We would be delighted to hear any suggestions you may have for improving the operation or performance of the machine 4 Are there any additional functions you would wish to see added Thank you for taking the time to complete this form Please return it to The IMS Manager Constant System
12. TO MARK This authorizes the application of the Certification Mark s shown below to the models described with model s identified on the correlation page of the Listing Applicant Constant Systems Limited Low March Northants NN11 480 United Kingeom Contact Name MrBobSincar Phone 44 0 1327 314 146 Fax 44 0 1327 314 147 Manufacturer Same Appicant Party Authorised to Apply Mark as Manufacturer Report Issuing Office intertex ETL SEMKO Leatherhead U K Report 05016948 Product Covered One Shot seres models O0 15 BA 0 20 O0 30 BA and O0 40 BA 152 series models 752 15 TS2 20 BA AA TS2 30 BA AA and TS2 40 BA AA TSS series models 785 15 TS5 15 EB GA and T 8 18 EC GA 755 series models 5 20 TS5 20 EB GA and 755 series models TS5 30 EE GA TSS 30 EB GA and TS5 30 EC GA TSS series models TS5 40 EE GA TS5 40 EB GA and 55 40 TS6 series models 56 15 756 15 TS8 15 EB GA and TS8 15 EC GA 156 series models TS6 20 BA AA TSG 20 EE GA TSG 20 EB GA and TSG 20 EC GA 187 series models TS7 40 EE GA TS7 40 EBIGA and TST 40 EC GA Description Disrupter Systems Standard s Electrical Equipment for RT A 1 Edition 2002 including revision Use Genera Requirements CAN CSA C22 2 No 1010 1 92 including amendment 2 February 1997 R1999 This document is the property
13. c MEIO Me H N Section Declaration of Confalfniby u u uu uuu u uuu uuu Section M Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 IA CONSTANT 2 Dc CONSTANT 42 Cell Disrupter Installation Operation amp Maintenance Overview Sequence of Operation As the name implies the Shot machine requires all the samples to be input and removed from the machine individually Assuming that a sample has just been disrupted and removed from the machine an overview of the operational sequence would be 2 5 6 Ensure that the PISTON DOWN lamp is ON Pipette the sample into the hole in the top of the cylinder Fit the cup with lid on into the top of the cylinder to collect the output Fit the Body and insert the Probe Press and hold the probe until the machine fires audibly Remove the Body and remove the sample which has collected in the cup Detailed Sequence of Operation This is a complete list of all operations although the ones that are indicated as Only for start up or similar will not need to be repeated for every sample if the machine is in constant use 1 2 Ensure that the Probe is in the machine Only for start up Switch on the power at the isolator switch as applicable Remov
14. e the body Rotate it until it is possible to lift the body away from the machine This is illustrated on DRG 3D 1243 Sheet 1 Remove the cup using the Cup Lifting Tool illustrated on DRG 3D 1243 Sheet 2 The cup may be warm if the machine has been in constant use for some time but it is never dangerously hot Check that the cup is empty To do this remove the lid using the Lid Lever and inspect the inside of the cup CAUTION It is the responsibility of the user to ensure the cleanliness of the input cavity the cup and the lid Observe any local or in house regulations concerning cleanliness and autoclave or 6 sterilise the HP Seal cylinder cup and lid as necessary Only if necessary The Dead Loss remains in the input cavity after the disruption cycle If it is important that the input cavity is cleaned before the next disruption follow the procedure entitled Removal and Replacement of the HP Seal in the Maintenance section Clean the HP Seal cylinder cup and lid Copyright Constant Systems Limited Section G 1 of 2 A p oom CONSTANT r s n i CONSTANT 222 Cell Disrupter Installation Operation 4 Manual CAUTION Do not fit the cup without the lid 7 Ensure that PISTON DOWN lamp is illuminated and that the PRESSURE SETTING indicator shows the desired pressure If the PISTON DOWN lamp is not illuminated press and hold the SLOW DOWN button until it illuminates
15. ect Alerts In accord with common practice three types of alert are used in this document e Warning failure to observe could cause death or injury e Caution failure to observe could cause damage to the equipment or process e Note hint about how to make the work easier etc WARNING Indicates a potentially hazardous situation risk of electric shock which if not avoided could result in death or serious injury WARNING Indicates a potentially hazardous situation in this case biohazard which if not avoided could result in death or serious injury WARNING Surface temperature could rise to a potentially hazardous situation WARNING Potential hazard of fire or combustion CAUTION Indicates a potentially hazardous situation which if not avoided may result in minor moderate injury Each alert declares which type it is Copyright Constant Systems Limited Section A 2 of 2 CONSTANT 1 CONSTANT 125 _ 44 SYSTEMS CONSTANT 222 ails ate Uy pee Section Safety Considerations Section B lui Fnit Section C Glossary of TOME Section D Partsof the Machine uuu uu u u u uuu etis Section E joo Section F Section G
16. g the cycle until the body is removed If the leak were heavy the sample could be seen coming from the vent shown as B and G on DRG 3D CD 1463 In the event of seal A leaking the product would pass to vent A and F WARNING The sample must pass as aerosol to a kill tank if the substance is harmful In the event of sample passing via vent H1 and H2 call a service technician Copyright Constant Systems Limited Section C 1 of 1 Safety Glossary OF Terms Parts of tlie cits I m THOUDIGSIOOUING uuu uu uuu u uuu luu P I Declaration of Confalfniby u u uu uuu u uuu Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 Section Section B Section C Section D Section E Section F Section G Section H Section J Section K
17. he user is responsible for any controls which may be required to ensure that this loading and unloading is done safely and without undue risk to the health and safety of the user and or other persons and or the environment Copyright Constant Systems Limited Section B 1 of 1 Section A Safety mae Section B 5 CANN AEN NM MD Section C GIOSSALY TOME M Section D Pants of the Section E joo ua M Section F Me Section G IP ge uuu Section H Maihtena E Mm Section J uu u u U Section K Spec MEIO Me H N Section Declaration of Confalfniby u u uu uuu u uuu uuu Section M Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 m CONSTANT r s n CONSTANT Cell Disrupter Installation Operation 4 m Manual C INSTALLATION Environment The machine is suitable only for indoor use in a clean dry dust and smoke free environment between 0 C and 40 C at an altitude up to 2000M with maximum relative humidity of 80 up to 31 C decreasing linear
18. le inactivated if head assembled incorrectly Secondary pressure containment Jet seal and target easily changed by non technical personnel with tools supplied Store under cover away from heat and a source of ignition Wear gloves and eye protection when handling or filling reservoir Ensure no oil spills on to electrical equipment Clean up any other spills immediately Disposal should be in accordance with local regulations and any environmental protection act components should be supplied by CSL or authorised agents Stainless steel trolley stand 510mm x 440mm x 385mm Stainless steel Operating manual One set of consumable spares Tools Section L 1 0f 1 _ d 2 CONSTANT 05 _ 44 SYSTEMS CONSTANT 222 INTOGUCTION eter ele ie ee Section A Section Eure E Section C Glossary TONS Section D Parts Of the Section E joo Section F Section G D aWiI gs uuu Section H E Mm Section J uu u u Section K hae Section Declaration of Conformity Sectio
19. ly to 50 at 40 C Location WARNING Do not locate the machine in any way that makes it difficult to operate the disconnecting device wall box The machine must be set on a flat and level surface positioned to allow access to the cable entry and a minimum space of 60mm each side for ventilation Electrical USA Canada only The fitted power cable must be connected to the local mains supply by a qualified person by connecting the Brown Wire to Live the Blue Wire to Neutral and the Green Yellow Wire to PE Ground using a 15 Amp fuse ref LP CC 15 Bussmann or equivalent The machine shall be isolated by a lockable 1 or 2 pole isolator capable of switching 20 Amps which must be located as close to the equipment as possible according to local codes and regulations A plug socket combination should not be used Electrical Europe Rest of the World A power cable with a moulded plug suitable for the local system is supplied for use with the machine Aerosol and Product Containment The liberation of aerosols and product into the atmosphere is prevented by internal sealing arrangements illustrated in DRG No 3D 1463 in section H Nonetheless venting may be required to prevent build up of pressure The two seals subjected to high pressure are marked A and B In order for the sample to pass from chamber C to chamber D successfully seals A and B must not leak In the event of seal B leaking the product is contained durin
20. n M Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 LOW MARCH DAVENTRY NORTHANTS NN11 4SD UK Tel 44 0 1327 314146 Fax 44 0 1327 314147 E mail constant score group com Website www constantsystem com EC DECLARATION OF CONFORMITY We Constant Systems Ltd under our sole responsibility declare that the product as listed below PRODUCT CATEGORY CELL DISRUPTER MODEL ONE SHOT conforms with principal safety objectives of the European Directive 97 23 EC Pressure Equipment Directive in accordance with conformity assessment procedure Module The pressure equipment constituting the Pressure Equipment Assembly consists of the following Disrupter Pump Category 1 Accumulator Article 3 3 SEP amp Hydraulic Operating Circuits Article 3 3 SEP Conforms with principal safety objectives of the European Directive 73 23 EC Low Voltage Directive Conforms with the protection requirements of European Directive 89 336 EC Directive This certificate also confirms that all tests specified in our build instructions have been satisfactorily completed The machine should at all times be operated as instructed in the Operating Manual Signed Robert Sinclair Title IMS Manager On Behalf Of Constant Systems Ltd Date MAY 2007 Copyright Constant Systems Limited SectionM 1 of2 a AUTHORIZATION
21. nt Systems Limited SectionH 1 of 7 a NOu23S OL H3J3H 494 3tivqS 01 S3 TOHI2 NI SON ILON 1139 LOHS JO NOLLVH3dO S9NIMOHS ano o1 an 113 8 9 e 0007 dna 4 NOILO3S OL M333M SLYVd 3HVdS OL 5355 SATOUID NI SON ILON JldWvs 10 TVAOW3H SNIMOHS d 54315 peration amp Maintei Ouo3S OL 51419 5 OL M3J3M 57055 NI SON ILON P T LM cg Distupter tallation O CONSTANT 4 a SYSTEMS Lr f Instal ti on isrupt r v 20642 UNES 5 e LI NOLLO3S OL H3J3 5 5 OL S3 TOHIO NI SON 310 71 35 3HnSS3Hd H9IH JO TVAOW3H SNIMOHS E tion amp Maintenance _ j L nstallation en Cell Disrupter NC _ gt 59 HA Er CONSTANT SYSTEMS Lro 13r JO NO WIS 5 HJIH HO3 LHOddNS sv 0357 2018 5 40 1 WOY 13040 ONIMOHS UVES CU cca Section A Safety Section luii Ln e Section C Glossary TONS
22. oke being approximately 0 4 ml of dead volume The One Shot cell disrupter will process material in fluid as well as non flowing samples such as cell paste plant leaves and tissue The equipment is used for bacteria fungi yeast algae plant and mammalian cells to disrupt cells extract DNA manipulate DNA and break down tissue It is quick and easy to use contained during operation and simple to clean Using this manual The drawings in this manual have all been collected together in Section H When reading the pages which contain references to drawings you may find it helpful to remove the page with the text and open the binder at the required drawing In this way you will be able to look at both the drawing and its associated text at the same time Before proceeding with installation please take time to read the information contained in this manual It contains important safety operational and maintenance information to help ensure that you get the best performance and long life from the machine This documentation satisfies all the requirements of the clauses UL61010A 1 Copyright Constant Systems Limited Section A 1 of 2 2 pp 1 EM 4 Cell Disrupter Installation Operation amp Maintenance Manual o Users are required to formally assess any and all risks associated with the processing of any sample materials prior to commencing work and are responsible for their own safety in this resp
23. on H Section J Section K Section L Section M CONSTANT SYSTEMS Lro f L SPECIFICATION FOR ONE SHOT CELL DISRUPTER The One Shot machine incorporates our unique disruption head and hydraulic operating and control components The disruption head is mounted in a stainless steel tray to avoid spillage The disruption head is easily dismantled for manual cleaning and pre cooling The Shot machine allows loading of solid sample cell paste or suspended sample QUANTITIES PROCESS TIME POWER REQUIRED COOLING CONTROL SYSTEM SAFETY MAINTENANCE HYDRAULIC OIL 15032 SPARES OPTIONS SIZE W D H MATERIAL SUPPLIED WITH Copyright Constant Systems Limited Quantity of sample that can be processed in each shot is dependant on the pressure rating of the machine and disruption head purchased These are shown in the table below ZOKPSI 5 1 10 5mls 20kPSI 1 16mls 10kPSI 1 20mls Manual loading of each shot takes approx 2 minutes It takes approx 30 seconds for the machine to operate and complete the disruption of the sample See Section C and rating plate on machine No provision is made for cooling but the removable parts can be pre cooled So as to use the cooling capacity of the metal parts Pressure control allows the disruption pressure to be accurately pre set before starting cycle Single push button control of disruption cycle Disruption cyc
24. part which drives the HP Seal upwards to compress the sample during the disruption cycle The word pressure can have different meanings in this context For Pressure operational use it is always qualified by the term Disruption Pressure Pressure Adjustment Knob The circular knob on the front of the cabinet which adjusts the Disruption Pressure Probe The rod which passes through the body and which is used to start a disruption cycle Slow Down SLOW DOWN button provides a means of moving the piston under manual control to the bottom of its travel usually for maintenance The piston moves while the button is held pressed When the piston has reached the bottom the yellow PISTON DOWN light illuminates Slow Up Copyright Constant Systems Limited The SLOW UP button provides a means of moving the piston under manual control to the top of its travel usually for maintenance The piston moves while the button is held pressed It is used only when the HP Seal is visible Section D1 of 1 A 1 CONSTANT 125 _ 44 SYSTEMS 22 rodu CION Section A Safety e Section B nsi Eure Section C Glossary OF TONNES Section D Main Parts of the Machine
25. s Limited Low March Daventry Northants NN11 4SD UK Or fax it to 44 0 1327 314147 Copyright Constant Systems Limited gt L d PTO CUCU 1 Safety ConslderallODS TE PH Glossary M Main Parts a the inse iis ria P DRAWINGS I m THOUDIGSIOOUING uuu uu uuu u uuu luu P I Declaration of Confalfniby u u uu uuu u uuu Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 1327 314 146 Fax 44 0 1327 314 147 Section Section B Section C Section D Section E Section F Section G Section H Section J Section K Section L Section M dec Co Ces ewe eer Safety Considerations Installatio Glossary Main Parts a the MACHING u u u Operation Drawings Maintenance Troubleshooting Specification Declaration of Conformity Log Book n of Terms Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44
26. sert the Cylinder Lifting Block in the gap and press and hold the SLOW DOWN button until the amber lamp comes on then release This will pull the piston downwards out of the cylinder which can then be lifted away from the machine To Remove the HP Seal CAUTION Take great care not to have fingers near to the hp cylinder or seal housing When the cylinder has been removed the HP Seal and piston are visible Drive the piston to the top using the SLOW UP button Release the button when the piston stops moving It will fall back slightly this is normal Switch off the power supply at the isolator This is essential to avoid the possibility of damage at the next step Fit the 14mm open ended piston spanner on the flats on the piston shaft You will later need to hold this spanner to prevent the piston shaft from rotating Hold the open ended piston spanner to prevent the piston shaft from rotating and turn the T bar anti clockwise to unscrew the HP Seal from the piston shaft The HP Seal can now be cleaned sterilised or renewed according to local procedures lt Copyright Constant Systems Limited SectionJ 1 of3 CONSTANT AUS SYSTEMS Lr f To Replace the HP Seal 1 6 T Ensure that the HP Seal and HP Piston mating faces are clean and then screw the HP Seal back into the piston by hand until the faces meet Fit the 14mm open ended piston spanner on the flats on the piston shaft Prevent
27. ss mallet Tap it hard enough to push out the jet To Fit a New Jet 1 Place the cup with base uppermost on a firm workbench 2 Place the new jet part way into the hole in the base of the cup It will fit easily for a short distance 3 Using the Jet Removal Holder on its side that is with the hole horizontal as a protector tap the Jet Removal Holder as hard as necessary to drive the jet into the base of the cup It should go fully into the cup until only the cone part is visible Removal and Replacement of the EDPM O Ring The expected life of the cup O Ring before leakage is approximately 100 cycles To replace the O Ring 1 Using the thumb and forefinger squeeze the O Ring to make it protrude to one side 2 Roll the O Ring off the jetthe cup Fitting of a new O Ring is the reverse of the above procedure Ensure that the O Ring is properly seated and not twisted in the groove NOTE On initial few cycles of this cup there may be small fragments of the EPDM O Ring escaping into the product or remaining in the cylinder However these are not detrimental to the product or process Should you find these fragments problematic for your application please run the cup using water for the first 10 cycles Copyright Constant Systems Limited Section J 3 of 3 UVES CU cca Section A Safety Section luii Ln e
28. ssure setting to clear jet if this does not help see Section D Jet and Cup Assembly Sample present under Cup or showing in telltales Replace cup and seal assembly No throughput of sample and stroke does not complete Try increasing pressure setting if this doesn t help Replace the blocked jet Seals and Leaks Refer to Remove the HP Seal under Maintenance FOR SERVICE CALL YOUR SUPPLYING AGENT Copyright Constant Systems Limited Section K 1 of 1 _ M Safety M Glossary TONS Parts of the Tipps u Declaration of Constant Systems Limited Low March Daventry Northants NN11 450 England Tel 44 0 132 Fax 44 0 1327 314 147 Section A Section B Section C Section D Section E Section F Section G Secti
29. the cup Copyright Constant Systems Limited Section G 2 of 2 Section A Safety Section Euro Section C Glossary TONS Section D Parts Of the Section E joo M Section F Me Section G Di WINGS tendo Section H uuu uu M Section J uu u u u u u uu u u u Section K Section Declaration of Confalfniby sse Section M Log Book Constant Systems Limited Low March Daventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 W 2 UM CONSTANT i t Cell Disrupter Installation Operation amp Maintenance Manual e T SYSTEMS Lro f gt 5 I DOES INDICATE THAT PISTON IS FULLY DOWN PISTON DOWN SLOWLY USE WHEN PISTON DOWN LIGHT CHANGING HP sEAL AND WHEN REMOVING HP CYLINDER FOR CLEANING PRESS TO MOVE OPERATING PISTONUP SLOWLY USE WHEN Copyright Consta
30. ventry Northants NN11 4SD England Tel 44 0 132 Fax 44 0 1327 314 147 F TOOLS Photo Diagrams of Tools amp Spares Kit Copyright Constant Systems Limited _ Section F 1 of 1 DESCRIPTION ITEM NO PART NO Tool box BO 1053 Tools amp Spares Cup Lifting Tool 1 KE1324 O Ring Set Shown as 2 amp 3 2 KE1742 Cup Lid O ring 2 BO 78 HP Oylinder O Ring 2 BO 78 Body O Ring 3 BO 305 Jet Removal Tool 4 KE1388 Cup O Ring x 10 part of No 11 5 BO 73 3 8 Tee Bar 6 BO 229 Socket 11mm A F 7 BO 232 Cup Lid Lever 8 KE1435 Jet Removal Block 9 KE1741 Flat Ground 14 A F Spanner 10 KE1468 Cup With Jet 11 KE2703 Cup Lid with O Ring Fitted to No 11 KE1255 Operating Probe 12 KE1395 3mm Allen Key 13 BO 140 HP Seal 14 KE2701 HP Oylinder Lifting Block 15 KE1474 User Manual Not Shown KE2656 Section A Safety Section luz En T Section C Glossary TONS Section D Parts of the Machine Section E Section F gru uwa Section G 5 Section H Maihtena E Mm Section J uu u u U Section K Spe
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