Hygiena Luminometer Manual
Contents
1. 5 2 Hardware and Software Requirements COMPONENT REQUIREMENT Des Pentium series processor or higher x86 32 bit and x64 64 bit supported Memory 256 megabyte MB RAM or higher Hard disk 600 megabytes MB of free space Microsoft Windows XP with Service Pack Operating system SP2 Windows Vista Windows 7 Windows 8 Communication USB or Serial RS232 port 5 3 Starting the Install Process NOTE If you have a previous version of the SureTrend software on your PC it is recommended that you make a backup of your data SureTrend software install will try to locate the previous version of SureTrend and perform an upgrade If you have an old version of SureTrend or dataSURE a new installation will be created The software is installed onto your computer by inserting the SureTrend CD that comes with the luminometer into your CD drive and following the installation script screen prompts If the install script does not automatically start the Auto Run feature on your computer has been disabled Open My Computer right click on the CD drive and select AutoPlay If this does not start the install program open the CD folder and run the Setup application NOTE You must have appropriate privileges to install software on your computer If you do not have sufficient permissions the software will not install 5 4 Using the SureTrend Software The user manual for SureTrend Data Analysis is delivered in soft copy only The user manu2. 15 15 17 18 19 19 19 20 20 5 4 USING THE SURETREND SOFTWARE 5 5 INSTALLING THE USB SMART CABLE DRIVER 5 6 CONNECTING THE LUMINOMETER TO THE PC 6 OPERATOR MAINTENANCE 6 1 CLEANING THE LUMINOMETER CASE 6 2 REPLACING THE BATTERIES 6 3 CLEANING AND REPLACING THE PROTECTIVE POCKET 7 TROUBLESHOOTING 7 1 LUMINOMETER BEEPS 7 2 TROUBLESHOOTING TIPS 7 3 LUMINOMETER ERROR CODES 8 UNIT WARRANTY AND RETURNS 8 1 WARRANTY DURATION 8 2 PARTICULAR EXCLUSION 9 GLOSSARY OF TERMS AND ABBREVIATIONS 10 TECHNICAL SPECIFICATION 11 CAUTIONS AND WARNINGS ITI 20 21 22 22 22 22 23 24 24 24 26 27 27 27 27 28 29 1 Introduction The luminometer is part of an ATP Hygiene Sanitation Monitoring System intended to detect ATP found in organic matter and microorganisms The Hygiena luminometer in conjunction with Hygiena s ATP and Micro snap test devices measures levels of contamination on surfaces water and product samples 1 Remove swab from test tube and collect sample Refer to instructions included with testing device 2 Place swab back in test tube 3 Break Snap Valve at top of swab by bending bulb Squeeze bulb twice pushing liquid reagent down swab shaft Shake test for 5 seconds Place test in the luminometer and close lid Hold the luminometer upright and press OK Reading will appear in 15 seconds au 1 1 What is ATP Adenosine Triphosphate ATP
3. ensure that the liquid in the testing device is at the bottom of the tube When the measurement is complete the RLU test result and overall pass caution fail result are displayed Pass Caution Fail gt Jf RLU i l RLU Results AE Prog i 109 30 lt Lower and Upper Thresholds Surface 1 Plan 1 Test 2 lt Test number Monday Prog number and Location gt Plan number and Name gt 13 25 2 4 2013 Time and Date TIP A failed test result x can be selected for retesting by pressing the button followed by the button to select the Program location see section 4 2 18 Now remove the testing device and dispose of it Previous test results can be viewed by pressing the and buttons see section 3 5 or the results statistical data can be obtained by pressing the button see section 3 6 TIP The luminometer can be recalibrated by removing the testing device closing the lid and then pressing and holding down the button for 1 second The luminometer will then perform a 15 second zero measurement 4 2 Performing a Retest When viewing a failed X test result see sections 3 5 and 4 2 the program location can be selected for retesting by pressing the button followed by the button NOTE It is only possible to select a program location for retest if the most recent test result for that location was a failure X Having selected retest mode signified by the icon
4. 5 Viewing Stored Test Results The luminometer can store a maximum of 2000 test results in its internal memory even when the batteries are low or removed Surface 1 To view these stored test results select the ss uz MEMORY menu option by pressing the button Scroll to MEMORY and press Then use the and buttons to scroll through the test results pressing the button to exit TIP After reviewing the stored test results a failed X Program location can be selected for retesting by pressing the button see section 4 2 108 306 Test 1 3 6 Viewing Statistical Data and Test Failures The test results database can be analyzed by selecting the STATISTICS menu option by pressing the button Scroll to STATISTICS and press i This will display a simple comparison of all the stored results The button also provides a direct shortcut to this function The and buttons can then be used to scroll through all of the failed X test results Press the amp button to exit 53 6 11 6 34 8 145 31 95 12 3 7 Erasing the Test Results Memory WARNING Once the test results have been erased from memory they are permanently deleted and can no longer be viewed or uploaded to SureTrend NOTE Once started the erase function cannot be stopped and will take about 30 seconds to erase a full 2000 results The entire test results memory can be cleared using the MEMORY ERASE fun
5. is an HaN organic molecule that is used by living 5N cells as the main source of energy o Oo 0 M Animal plant bacterial yeast and mold 9 0 p 0 N cells produce and break down ATP in _ we 6 a order to drive a number of biological OH OH processes It is the main energy transfer molecule in the cell The presence of ATP on a surface indicates that the surface has not been adequately cleaned and has the potential to harbor and support bacterial growth The residue may also contain hazardous material including potential allergens Therefore ATP is an ideal indicator of surface cleanliness 1 2 How Does the Luminometer Work The luminometer detects ATP using a chemical reaction Luciferin Luciferase ATP Light A sample is collected using a Hygiena ATP device see section 1 4 Surface tests come with a buffer moistened swab bud that aids in the removal of any biological material ATP on either a wet or dry surface Once the sample is collected and exposed to the chemistry buffer in the Hygiena device the ATP contained inside the collected cells will be released ATP released from inside these cells along with any free ATP picked up from the surface by the swab is now available to react with the chemistry in the bulb of the device When the Luciferin Luciferase enzymes are exposed to ATP light is produced The light is detected and measured by the luminometer The amount of light detected is directly proportional to
6. the quantity of ATP collected and accordingly is proportional to the amount of residue in the tested sample The higher the reading the higher the amount of contamination in the sample 1 3 What is an RLU The luminometer displays results in Relative Light Unit RLU values The light produced from the Luciferin Luciferase and ATP reaction in the swab is emitted in the form of photons A photon is an elementary particle and the basic unit of light The luminometer detects these photons and displays them directly as RLU values The more light detected by the luminometer the greater the RLU value The quantitative RLU reading is then compared against user programmable thresholds to provide an overall qualitative pass y caution or fail X result The luminometer detects total ATP not just ATP from bacteria yeast and mold but also the ATP from anything organic in the sample Therefore an RLU value is not the same as a microbial colony forming unit CFU Since the luminometer is detecting total ATP it is unknown whether the RLU result displayed by the luminometer is due to the detection of microbial ATP residual ATP or a combination of both Therefore a comparison cannot be drawn between RLU values from ATP and standard plate counts SPC or rather RLU does not equal CFU True microbial contamination can only be determined by a standard plate count method that can take up to 48 hours for results The luminometer is capable of providin
7. CAUTION Do not force testing device into the unit Do not attempt to insert any object other than an approved testing device into the unit A CAUTION Ensure that the testing device is clean and dry before inserting it into the unit Keypad Buttons A CAUTION Do not use excessive force when pressing any of the buttons on the unit s keypad RS232 or USB Connector WARNING Computer equipment connected to the RS232 connector N at the top of the unit must conform to BS EN 60950 IEC950 standards Unit Casework and Serviceability A WARNING There are no Operator serviceable parts inside the unit Removal or opening of the unit s casework will void the warranty 29 WEEE Directive Compliance The luminometer should be disposed of in accordance with the European Union WEEE Directive 2002 96 EC on Waste m Electrical and Electronic Equipment WARNING Do not dispose of this product into unsorted A municipal waste or a public landfill Please contact your local distributor for details of how to correctly dispose of this product Regulatory Limitations of Use The luminometer has been designed to meet the following general safety and EMC requirements e Low Voltage Directive 73 23 EEC e EMC Directive 89 336 EEC Safety e BS EN 61010 1 2001 IEC 61010 1 2001 e UL61010B 1 e CAN CSA C22 2 1010 1 92 EMC EN 55022 1998 Class B FCC CFR47 Pti5 109 Class B EN 61000 4 3 1996 ESD EN 61000 4 2 1995 Al The luminometer is manufactured
8. LCD Contrast The contrast of the LCD screen is factory set at its optimum level for normal operating conditions However in extremes of temperature the display may appear too dark or light This setting can be manually adjusted by selecting the LCD CONTRAST menu option by pressing the button Scroll to CONTRAST and press Then use the or button to increase or decrease the contrast level followed by the button to store the new setting A darker 1 to 5 at Vad Yes Normal 0 oo 7 lighter 1 to 5 3 10 Quick Boot The luminometer will turn on when you press the button The luminometer will perform a standard calibration in 15 seconds and will then be ready to perform readings Quick Boot can be turned off and the luminometer will perform a more in depth system check the next time it is turned on The more in depth system check takes 60 seconds NOTE The more in depth system check is typically used for diagnostic purposes and not necessary for normal use To change the Quick Boot setting select the QUICK BOOT menu option by pressing the k button Scroll to QUICK BOOT and press button to switch between ON and OFF OFF ON Exit 3 11 Standby Mode The Standby Mode is used to control when the luminometer should automatically turn off when not in use This is designed to conserve battery power The default is 20 minutes If no buttons are pressed within the time period the luminomete
9. This Manual Copyright 2012 by Hygiena LLC All rights reserved No part of this manual may be reproduced stored in a retrieval system or transmitted by any means or in any form without the prior permission of Hygiena LLC The information contained in this manual is subject to change without notice Hygiena LLC assumes no responsibility for any errors that may appear in this document This manual will describe the specifications and general operations of the SystemSURE Plus v2 and EnSURE v2 luminometers For older luminometers refer to the operators manual that shipped with the luminometer for specific operations Document SystemSURE Operators Manual V3 0 2013 Copyrights and Trademarks Worldwide Patents Pending Hygiena EnSURE SystemSURE Plus SystemSURE I Ultrasnap SureTrend and dataSURE II are trademarks of Hygiena LLC Operating Precautions and Limitations of Use IMPORTANT Hygiena s products are designed and constructed to be safe and without risk to health when properly used in accordance with the supplied documentation and when the operating precautions outlined in this manual are fully observed It is essential that the user of this manual is aware of the potential hazards associated with the unit and its accessories All operators should be familiar with the safety precautions and warnings given in this manual before attempting to operate the unit If the unit is used in a manner which is not specified b
10. aged with the luminometer 3 1 User ID The luminometer can store a list of 201 User IDs User 0 to 200 Each test result will be tagged with the currently selected User ID Select the User ID menu option then use the and buttons to scroll through the list of User IDs then press the button to select a User ID or the button to cancel the selection TIP User 0 is the default selection if no User ID list has been defined 3 2 Setting up Program Test Points and Pass Fail Limits The luminometer has the ability to store a list of 5001 unique Program test points Prog 0 to 5000 each with its own upper tr and lower 4 pass fail result threshold values Program test point location names can only be programmed into the luminometer through the SureTrend software Program pass fail thresholds can either be set up on the luminometer or set using the SureTrend software If a location name is entered and threshold set in SureTrend and then downloaded to the luminometer the threshold values can t be changed via the luminometer This is a security feature and prevents inadvertent changes to the thresholds after being set in SureTrend TIP Prog 0O has a special function whereby the threshold values defined for Prog 0 are used as the initial values when setting up the other Prog thresholds Setting Prog 0 with your typical threshold values will save time when setting up the other Programs The luminometer comes with pass fa
11. al can be found on the SureTrend software CD The soft copies are in PDF and XPS formats In order to view or print these documents you will need to install Adobe Acrobat Reader or Microsoft XPS viewer If you are running Windows 7 or later the Microsoft XPS viewer is already installed 20 5 5 Installing the USB Smart Cable Driver If your luminometer came with a USB to RS232 cable you need to install the SystemSURE USB Smart Cable device driver The device driver should be installed when you install the SureTrend software see section 5 3 However if needed you can follow the instructions below to install the SystemSURE USB Smart Cable device driver hs NOTE Insert the Smart USB cable into an available USB port on your computer If the cable is already connected to your computer remove the cable and plug it in again This will cause Windows to detect the new hardware and start the Found New Hardware Wizard If the Found New Hardware Wizard does not start check the following a You have sufficient permissions to run the wizard b Verify there are no existing hardware problems in device manager c Verify the Universal Plug and Play service is running Follow the Found New Hardware Wizard steps to install the device driver If your computer is connected to the Internet and you are running Windows 7 or a later version you can search the Microsoft Windows Update site for the device drivers If your
12. arranged with the following buttons X OOG EME Power on off button Menu select and Exit button Up increase button Start accept button Down decrease button Program select button Statistics shortcut button The functions of the keypad are explained in detail in the following sections of this manual TIP Pressing and holding down the or button will make it auto repeat The longer the buttons are held down the faster it goes Opening the lid reveals the following internal features RS232 connector cover Protective pocket and sample insertion port For details on the RS232 USB connector and protective pocket refer to sections 5 6 and 6 1 respectively 2 2 Installing Batteries The unit is designed to operate from both non rechargeable alkaline batteries and rechargeable Nickel Metal Hydride NiMH or Nickel Cadmium NiCD batteries Battery Nominal Relative Technology Cell Voltage Capacity a ES 8 De Alkaline 1 5V 1 0 NiMH 1 2V 0 6 NiCD 1 2V 0 5 The unit requires two batteries of size AA LR6 or E91 TIP For best results always use a quality brand of Alkaline battery and replace as soon as the batteries become low see section 2 5 A WARNING Do not mix batteries of different technologies or use recharged alkaline batteries as these are prone to overcharging and leaking which may cause permanent unit damage and malfunction The batteries are fitted by unclipping the bat
13. computer is NOT connected to the Internet or you are running Windows XP you need to place the SureTrend software CD into an available drive The Smart USB Cable device drivers are located on the CD Instruct the Found New Hardware Wizard to search the CD when prompted the USB drivers are Windows certified for Windows XP Windows Vista Windows 7 and Windows 8 21 5 6 Connecting the Luminometer to the PC Now connect the serial interface cable as supplied with the luminometer between the luminometer and one of the RS232 or USB ports on your PC Fold back rubber Insert small RJ10 plug Connect other end to cover to access into luminometer s the PC s COM port or RS232 socket RS232 socket USB port depending on the Cable included with your luminometer NOTE Select the appropriate RS232 or USB cable most suitable for your PC A USB to RS232 adapter can also be used Please contact your local distributor for further details 6 Operator Maintenance The luminometer does not require any specific routine operator or service engineer maintenance 6 1 Cleaning the Luminometer Case Clean the luminometer case when required using a dry or slightly damp cloth only A WARNING Never clean the luminometer using a wet cloth or washing it with water A CAUTION Do not use solvents or other strong cleaning solutions as these may attack and deform the luminometer s plastic components and seriously degrade its performance 6 2 R
14. ction by first selecting the amp then using the up or down arrows to select the MEMORY menu option then press to select and then pressing and holding down the button for 2 seconds The display will show the total number of stored results to be erased To accept and start the erase function press and hold down the button for 1 second or press any other button to exit the option 3 8 Setting the Clock Time and Date To set or change the clock time date and format select the CLOCK menu option by pressing the button Scroll to CLOCK and press Then use the and buttons to change the time and date value followed by the button to accept each new value First the time is set style gt hours gt minutes which can be configured as either a 12 hour clock select AM or PM or a 24 hour clock select 24H CLOCK A v AM PM or 24H er A 7 minutes Next set the date format which can be configured for either European format select D M Y for day month year or American format select M D Y for month day year A 7 D M Y or M D Y A v day D M Y or month M D Y A 7 month D M Y or day M D Y A year TIP Pressing the button at any point will exit the clock set up mode leaving the time and date unchanged NOTE The clock does not have automatic daylight saving adjustment If this is required the time must be manually changed when necessary 13 3 9 Adjusting the
15. d Measurement result y Incorrect use of testing device is always zero RLU v Luminometer not in upright position when measurement taken or is much lower v Testing device is out of date expected v Luminometer used in an unstable thermal environment turn the luminometer off and back on v Protective pocket or testing device not fully inserted into luminometer v Protective pocket dirty or severely scratched x Protective pocket damaged x Luminometer damaged or faulty Measurement result y Incorrect or inconsistent use of the testing device is higher than v Lid not fully closed expected v Luminometer used in an unstable thermal environment turn the luminometer off and back on x Luminometer casework or lid damaged or faulty RS232 interface v Wrong interface cable being used does not appear to v Cable connector incorrectly inserted into luminometer be working v Cable connected to wrong port on PC v Wrong PC software being used v PC software incorrectly installed or wrong options selected x Cable or connectors damaged or broken x PC serial port or system software faulty x Luminometer damaged or faulty 25 7 3 Luminometer Error Codes During normal operation the luminometer performs LL various self checks on its internal components If a problem is detected the display will show an error 7 az n u m be r aii Error Code Possible Causes E1 Not applicable on the luminometer E2 v The luminometer is being used outside of the Temp
16. ed or faulty 26 8 Unit Warranty and Returns The Supplier warrants the luminometer when purchased new to be free from defects in materials and workmanship and will repair or replace at their discretion any luminometer which used under proper conditions exhibits such defects Under the terms of this warranty the product must be returned in the original packaging transportation prepaid with a copy of the Proof of Purchase to your local distributor Contact your local distributor to receive authorization to return the instrument and enclose a detailed description of the problem 8 1 Warranty Duration This warranty is provided to the original purchaser for one year from the date of purchase In no event will Hygiena be liable for indirect incidental or consequential damages the original user s remedies being limited to repair or replacement of the unit at the manufacturer s option 8 2 Particular Exclusion Unauthorized modification of any part of the luminometer or the use or attachment of any peripheral not supplied or specified by Hygiena will void this Warranty A WARNING Use only the accessories and consumables supplied by Hygiena The use of any non Hygiena supplied accessories and consumables will invalidate the warranty 9 Glossary of Terms and Abbreviations ATP fmol Femtomole 10 15 moles NiMH NickelMetalHydriderechargeable batteries IBM compatible personal computer of Pentium 1 specification or h
17. eplacing the Batteries For best results the batteries should be replaced when the low battery _ warning icon is flashing For details on how to fit new batteries see section 2 2 Take care not to mix the old batteries with the new ones IMPORTANT Always dispose of old batteries in accordance with your local authority regulations 22 6 3 Cleaning and Replacing the Protective Pocket A WARNING Always turn off the luminometer before removing the protective pocket The luminometer is designed with a special protective pocket which can be removed for cleaning or replacement if required To remove the protective pocket open the luminometer s lid tightly grasp the finger grip of the pocket gently pull the pocket upwards and 1 Grasp finger 2 Pull pocket upwards 3 Remove pocket grip A CAUTION Great care should be taken when removing the pocket not to damage it or the surrounding casework Do not use excessive force Never use a tool to pry out the pocket Carefully clean the interior of the pocket using water or a very mild detergent solution ensuring that the pocket is completely dry and clean before putting it back into the luminometer A WARNING Do not use solvents or other strong chemicals as these will degrade the clear optical section of the pocket and affect the performance of the luminometer To replace the pocket carefully insert it into the luminometer ensuring that it is correctly orientated and t
18. erature out specified operating temperature range see of range section 10 v The luminometer has been stored in an environment which is outside of its specified operating temperature range x Luminometer damaged or fault E3 v Luminometer environment unstable or used in Erratic an area of high electromagnetic noise measurement v Luminometer tilted while measurement being performed v Luminometer lid damaged and allowing light in v Protective pocket dirty or severely scratched x Luminometer damaged or faulty E4 v The user configurable settings are undefined User settings check and re set the CLOCK format User undefined Plan and Prog data E5 v Program thresholds not defined and have been Program Test reset to the default values 100 and 30 Plan or User ID v Test Plan data not defined undefined v User ID not defined E6 v Power the luminometer off and then back on Calibration self v Luminometer operating environment unstable checks failed v Protective pocket dirty or severely scratched v Lid not fully closed x Lid seal damaged x Protective pocket damaged x Luminometer damaged or faulty E7 v Batteries are low or loose Internal memory Luminometer memory damaged or faulty failure E8 v Batteries are flat or loose Internal reader x Luminometer sample reader is damaged or fault faulty E9 v Batteries are flat or loose Internal error v Luminometer dropped or subjected to shock or vibration x Luminometer damag
19. g results in 15 seconds offering a rapid real time approach to hygiene sanitation monitoring The luminometer is not meant to replace microbial testing It can be an effective tool when incorporated into a Hazard Analysis Critical Control Point HACCP Good Manufacturing Practices GMP or sanitation plan 1 4 Test Devices and Accessories US2020 Surface total ATP test device SuperSnap SUS3000 High sensitivity total ATP test suited for allergen prevention applications AquaSnap Total AQ100X Water total ATP test device AquaSnap Free AQ100FX Water Free ATP test device ontamination CCK 4 Cuvette based water testing kit Control Kit MicroSnap MS EB Enterobacteriaceae test Enterobacteriaceae MicroSnap MS COLIFORM Coliform test Coliform Test MicroSnap E coli MS ECOLI E coli test Test MicroSnap Total MS TOTAL _ Total Viable Count test ZymoSnap ALP ZS Alkaline Phosphatase test CrossCheck CX3000 Acid Phosphatase test Calibration Kit PCD4000 Positive and negative rod for validating luminometer calibration ATP Positive CK25 25 vials of freeze dried ATP to verify Control Kit efficacy of ATP test devices See the Hygiena website for a complete list of test devices accessories and other products www hygiena com 2 Luminometer Description Luminometer components Front 2 1 Keypad Symbols Liquid crystal display Neck strap anchor Serial number and Model revision Battery compartment The keypad is
20. he lid closed and no testing device present the luminometer will automatically perform a recalibration cycle under any of the following circumstances e The user presses and holds down the button for 1 second e The luminometer is used in an environment where the temperature changes significantly typically more than 5 C 2 8 Low Memory Warning The luminometer can store up to 2000 test results in its internal memory When the memory is more than 95 full i e less than 100 tests remaining 95 the following warning symbol is displayed When the memory becomes completely full no additional tests can be performed until the memory is either erased or uploaded to the PC see sections 3 6 and 5 respectively Test can be performed if Save Test is turned off see section 3 13 12 51 1 1 2000 3 Set up Menu Options With the luminometer turned on the Set up Menu can be accessed by pressing the button Use the and buttons to scroll through the menu list and then press the button to select the required menu option or the button the exit the menu The following 11 menu options are available User ID Test Plan Program Statistics Memory Clock LCD Contrast Quick Boot Standby Mode Bold Text Save Tests NOTE SureTrend software is required for sections 3 1 to 3 3 For further details refer to the separate SureTrend User s Manual located on the SureTrend software CD that is pack
21. hen push it fully down until it clicks into position 1 Insert pocket in 2 Push fully down 3 Push RS232 the orientation until it clicks into cover back under lip shown place A WARNING Do not use excessive force when replacing the pocket If the pocket jams during insertion remove it fully check for obstructions or damage and then try again Finally ensure that the black rubber RS232 cover is tucked in under the lip and that the lid can be fully closed see section 5 6 23 7 Troubleshooting This section lists typical problems that might be encountered when using the luminometer and the possible causes and remedies Some problems can be rectified by the operator and others may require technical assistance Action Required This indicates a cause which can be rectified by the operator gt een Contact your local distributor for further assistance TIP Most problems are likely to be transitory and can be cleared by pressing the button or by turning the luminometer off and then back on In some cases you may need to remove the batteries for 30 seconds and then reinsert them If any problem persists please contact your local distributor for technical assistance giving full details of the error code 7 1 Luminometer Beeps During normal use the luminometer emits a variety of different beeping sounds Beep Type Possible Causes Short high v Luminometer turned on or off pitched tone v Sample measure
22. hich will damage the luminometer TIP Always store the luminometer in a cool dry place when not in use because elevated temperatures will shorten the battery life 2 6 Power Saving Standby Mode If the luminometer is on but has not been used for 19 minutes it will beep every 10 seconds for 1 minute and then turn off If any key is pressed during that time the luminometer will reset the Standby Mode countdown The Standby Mode settings can be changed see section 3 11 To turn the luminometer back on simply press the 6 button 2 7 Internal Calibration When the luminometer is turned on see section I 2 3 it performs an internal calibration as the I display counts down from 15 to 0 seconds NOTE During the calibration sequence there must x not be a testing device in the luminometer and the lid must remain closed If the f icon is shown with the arrow flashing open the lid and remove the testing device from the luminometer If the I icon is flashing close the lid If it is still flashing make sure the rubber flap covering the RS232 port is tucked under the lip and is flat TIP The button can be pressed at any time to access the set up menu options see section 3 When the calibration is complete the luminometer is ready to perform a sample measurement T For details of how to perform a sample measurement refer to section 4 NOTE When the luminometer is ready to performa Proge 188 38 measurement with t
23. igher running PC l Windows 98 or later Measurement value in RLUs Result Measurement pass caution l or fail X RLU Relative Light Units unit of measurement Serial communications protocol for connecting the unit to a PC used for uploading test results data USB Universal Serial Bus used to connect computer peripherals to a PC The SystemSURE Plus EnSURE units 27 10 Technical Specification General Unit Details Batteries Unit dimensions W x H x D Unit weight including batteries Operating temperature range Relative humidity range Storage temperature range Relative humidity Range Measurement range Measurement resolution Measurement time Measurement noise User IDs Programmable result thresholds Programmable location names Test plans Results memory size Serial interface Battery Size 2 off Battery Types Non rechargeable Rechargeable externally charged Battery Capacity for 2600mAh Standby mode at 20 C Continuous reading 28 72mm x 191mm x 32mm approx 260g 5 C to 40 C 20 to 85 non condensing 10 C to 40 C 20 to 95 non condensing 0 to 9999 RLUs 1 RLU 15 seconds 5 or 5 RLUs 200 IDs of 16 characters 5001 programs 5001 names of 20 characters 100 plans of 251 programs 2000 tests EIA 232 compatible AA LR6 or E91 nom 1 5V Alkaline nom 1 2V NiMH or NiCD min 6 months min 2000 tests 11 Cautions and Warnings Operating E
24. il thresholds of 10 RLU for pass and 30 RLU for fail These pass fail thresholds were determined based on a comprehensive study of ATP food residue and microorganism samples To receive the report or more information on setting up pass fail thresholds contact Hygiena or visit our Question and Answer section at www hygiena com To set the Program result thresholds on the luminometer first select the PROGRAM menu option by pressing amp Scroll to PROGRAM and press 9 Then use the and buttons to scroll through the list of Programs followed by the button to select the Program to be changed 10 PROGRAM 182 387 Next use the and buttons to first change the value of the upper threshold tt followed by the button to select the lower threshold use g Use the and buttons to change the lower value followed by the button to store the new values A v upper threshold tt El A 7 lower threshold 8 NOTE If the selected Program location does not have any thresholds defined it must be set up using the PROGRAM menu option before the Program can be used 3 3 Test Plans The luminometer can also store up to 100 individual Test Plans Each Test Plan can contain a list of up to 251 test locations specified in the order in which they would normally be tested Having selected the TEST PLAN menu option use the and buttons to scroll through the list of Test Plans and then press the button t
25. ment started v Communications established with PC v Results memory being erased Long high v Luminometer calibration self checks complete pitched tone v Sample measurement complete v Results memory erase complete Long low v Invalid date entered pitched tone v Program thresholds not set up v Invalid program threshold limits entered v Attempting to select a non failed pass caution result for retesting Two short v Calibration self checks required remove testing device and close high pitched the lid tones Three short v Clock set up required high pitched v Batteries are low tones v Memory full warning v Memory erase requested v Luminometer error see section 7 3 7 2 Troubleshooting Tips If the luminometer appears to be malfunctioning for any reason carry out a thorough check for any obvious damage to the case lid LCD screen battery contacts etc caused by dropping or excessive physical mishandling 24 The following table lists typical symptoms and their possible causes Luminometer will not v Batteries are low turn on when the y Batteries are the wrong type button is pressed y Batteries incorrectly inserted x Luminometer or keypad damaged or fault Luminometer will not v Luminometer is busy performing a reading or calibration self check turn off when the operation button is pressed y Luminometer lockup remove the batteries for 30 seconds then insert them again x Luminometer or keypad damaged or fault Lumi
26. nd Location gt Plan number and Name John Smith User Name Various keypad options are now available as detailed in each of the following sections Button Action Section F View previous test results Show statistical result data Turn luminometer off 17 4 1 Taking a Sample Measurement IMPORTANT Please refer to the testing device data sheet and kit insert for full details on how to use the testing device NOTE Always allow the luminometer sufficient time to acclimatise to any change in environment temperature or humidity before taking sample measurements To perform a sample measurement follow the steps below 1 Swab the sample area and activate the testing device see kit insert for details 2 Open the luminometer lid insert the testing device into the luminometer and close the lid 3 Holding the luminometer upright press the button and wait 15 seconds for the result to be displayed A WARNING Always ensure that the exterior of the testing device is clean and dry before inserting it into the luminometer Never insert anything other than a Hygiena testing device into the luminometer Never insert a device into the luminometer when the protective pocket is removed see section 6 3 While the measurement is being performed the display counts down from 15 to 0 NOTE For consistent results always keep the luminometer upright and steady while it is performing a measurement to
27. next to the Prog perform the sample measurement in the usual way using a new testing device see section 4 1 Once the retest measurement is complete the original failed test result is marked with the icon and the new test result is tagged with the icon J RLU E Li Prog 1 109 306 Prog 1 108 30 Surface 1 Surface 1 Plan 1 Test 3 Plan 1 Test 4 Monday Monday 13 40 2 4 2015 13 58 2 4 2013 NOTE Retest mode is automatically deselected once the new measurement is complete or can be manually cancelled by pressing the P button and selecting a new program location 5 Installing SureTrend Data Analysis Software The test results stored in the luminometer s memory can be uploaded to a PC using the SureTrend Data Analysis software The software is supplied on the CD with the luminometer 5 1 Conditions of Use As Hygiena LLC has no control over the specification state or use of any computer equipment on which this software is installed or used the following Conditions of Use apply 19 IMPORTANT The software is provided as is without warranties of any kind either expressed or implied including warranties of merchantability or fitness for a particular purpose Hygiena LLC shall not be liable for any loss of profit loss of use loss of software loss of data interruption to business nor for indirect special incidental or consequential damages of any kind whether under this agreement or not
28. nometer turns v Batteries are low off unexpectedly v Batteries are loose within the battery compartment v Luminometer dropped or subjected to shock or vibration v Luminometer not used for 10 minutes and automatically enters standby mode x Luminometer damaged or fault Luminometer beeps v Batteries are low when turned on but x Luminometer or display damaged or faulty nothing is displayed Clock reverts to v Batteries have been replaced 12 00 01 01 2000 v Batteries are low v Batteries are loose within the battery compartment v Luminometer dropped or subjected to shock or vibration x Luminometer damaged or fault The display appears v Luminometer is too hot or too cold washed out or very v LCD contrast incorrectly adjusted see section 3 9 dark v Luminometer is being used in inappropriate lighting conditions x Luminometer or display damaged or fault Display segments v Display window is dirty missing or garbage x Display window is scratched or dented displayed x Display or luminometer damaged or fault Keypad button has v Some buttons only work when selecting particular luminometer functions no effect when v Lid not fully closed pressed x Keypad or luminometer damaged or faulty Luminometer lid will v Protective pocket incorrectly or not fully inserted not close properly or v RS232 connector cover is interfering with the lid see section 6 3 springs open during v Testing device incorrectly or not fully inserted use x Lid or luminometer casework damage
29. nvironment and Electrostatic Precautions WARNING Do not use the unit in any area which has been or is AN thought to have been exposed to explosive or flammable gases or vapours A CAUTION Avoid operating the unit in direct sunlight Never leave the unit in direct sunlight even when turned off CAUTION Do not expose or operate the unit in extremes of temperature see section 10 and minimise any exposure to electrostatic charges Unit Handling and Use CAUTION Care should be taken not to drop the unit or subject it to any form of rough physical handling both during normal use and during storage and transportation A CAUTION To avoid the risk of injury or choking never use the unit neck strap in situations where it could get caught in machinery A CAUTION To prevent dust and dirt ingress always keep the unit lid closed when not inserting or removing a testing device Batteries A WARNING Use only non rechargeable alkaline batteries or rechargeable NiMH NiCD batteries of types specified in section 10 A WARNING Do not use batteries with individual cell voltages greater than 1 65V as this will cause permanent damage A CAUTION All batteries should be disposed of in accordance with your local regulations Use and Insertion of Testing Devices CAUTION Refer to the testing device data sheet and kit insert for details before using the testing device and observe all federal state and local environmental regulations A
30. o select the new Plan or the button to cancel the selection TIP Plan O allows any of the 5001 test locations to be tested in any order and is the default selection if no Test Plans have been defined or selected An example set of Test Plans for a typical week might be Monday Tuesday Wednesday Thursday Friday Prog 1 Prog 2 Prog 3 Prog 7 Prog 10 Prog 8 Progi7 Prog 3 Prog 2 Prog 1 PROGRAM Prog 8 Prog 8 Prog 9 Prog 7 Prog 6 Prog 3 Prog 5 Prog 2 Prog 4 Prog 1 Prog 10 11 Test Plans are defined by using the SureTrend Windows software Please refer to the SureTrend User s Manual located on the SureTrend software CD for full details see section 5 To select a Test Plan use the TEST PLAN menu PL option NOTE If Test Plan Plan 0 is selected or no Test Plans have been defined any of the 5001 Program da nad locations can be selected To select a Program location press the button then use the and buttons to change the Program Prog to the desired Program location followed by the button to accept the new location or the button to cancel the selection TIP When selecting a Prog the display also shows the time and date that the Program location was last tested 3 4 Establishing ATP Pass Fail Limits For instructions on establishing Pass Fail limits refer to the implementation guide included with your system or visit the resources available on www hygiena com 3
31. ormally This will allow all text to be displayed even if the text is maximum and all wide characters To change the Bold Text setting select the BOLD TEXT menu option by pressing the button Scroll to BOLD TEXT and press button to switch between ON and OFF OFF 3 13 Save Tests By default any test performed on the luminometer is recorded to the luminometer s non volatile memory Even if you take the batteries out the results of the test are retained in the luminometer s memory 15 In some cases such as training calibration checks etc users may want to discard test results If Save Test is set to OFF all tests taken in this mode will not be recorded IL NOTE The Save Tests mode will be set back to ON when the luminometer is turned off and back on again When Save Tests mode is set to OFF eres 108 308 RESULTS NOT SAVED will appear at the bottom of the LCD To change the Save Tests setting select the SAVE TESTS menu option by pressing the button Scroll to SAVE TESTS and press button to switch between ON and OFF OFF ON Exit 16 4 Sample Measurements and Test Results With the luminometer turned on and having completed its internal calibration self checks it is ready to perform a new sample measurement and the display shows the following details Insert sample icon gt A Prog i 10 300 lt Lower and Upper Thresholds Surface 1 Plan 1 Monday Prog number a
32. r will turn off If any button is pressed or a reading is performed the Standby Mode countdown timer is reset to zero 14 If the bell icon M is enabled the luminometer will begin beeping every 10 seconds for 1 minute as a warning before turning off For example if the Standby Mode time is set to 20 then if the luminometer is not used for 19 minutes it will start to beep every 10 seconds for 1 minute If no action is taken the luminometer will turn off If any button except the power button is pressed the countdown will restart To change the Standby Mode setting select the STANDBY MODE menu option by pressing the amp button Scroll to STANDBY MODE and press button Then use the or button to increase or decrease the Standby Mode duration followed by the button to store the new setting Standby Mode duration can be from 5 to 60 minutes in increments of 5 minutes Then use the or button to enable or disable the warning beeps This will toggle the bell icon from enabled W to disabled amp Press the button to accept the settings A v increase or decrease duration 5 to 60 set duration A v enable or disable warning beeps set warning state 3 12 Bold Text The luminometer displays key information on the LCD in bold to improve legibility In some cases the number and size of the text will exceed the allotted space on the LCD If bold is set to OFF all text on the luminometer will be displayed n
33. tery cover on the back of the unit and inserting both batteries with their positive terminals pointing towards the top of the unit A CAUTION Take care not to insert the batteries in the wrong direction as this may cause permanent damage to the unit s internal electronics 1 Remove cover 2 Insert battenes 3 Replace cover When the batteries are inserted correctly the unit will automatically turn on and enter the clock set up mode see section 3 8 2 3 Turning the Luminometer On and Off To turn the unit on press the 6 button The unit will beep once and display the power up self check screen NOTE If the batteries are too low the unit may turn on and back off again automatically or may not turn on at all If this happens replace the batteries immediately The unit will then perform an internal 15 second calibration see section 2 7 NOTE If the clock is not set the unit will automatically enter the time and date set up mode see section 3 8 before performing its calibration TIP It is recommended you synchronize the unit after changing the batteries before use This will allow SureTrend software to set the date time and format to the computer s date time and format TIP To activate the backlight features press and hold down the button The backlight uses more power and will drain the battery faster To turn the luminometer off press the button The luminometer will beep once and the displa
34. under ISO 9001 controls Declaration of Conformity The luminometer has been designed in accordance with and satisfies the requirements of article 11 of the Low Voltage Directive 73 23 EEC as realigned by 93 68 EEC on the harmonisation of the laws of the Member States relating to electrical equipment designed for use within certain voltage limits to the essential requirements of BS EN 61010 1 2001 The luminometer has been type tested by EMC Projects Limited a UKAS and CAA approved test facility and UK appointed Notified Body and issued with a Certificate of Compliance No 6349 07 to the following EMC standard EN61326 1997 Covering Radiated Emissions EN 55022 1998 Class B Radiated Emissions FCC CFR47 Pt15 109 Class B Radiated Immunity EN 61000 4 3 1996 Electrostatic Discharge EN 61000 4 2 1995 A1 Satisfying the EMC Directive s 89 336 EEC and 92 31 EEC as realigned by 93 69 EEC 30
35. y the manufacturer the protection provided by the equipment may be impaired Please read this manual in its entirety and the Operating Precautions and Limitations of Use at the end of the manual before using the Luminometer The following symbol is used in this manual AN Description CAUTION WARNING Table of Contents 1 INTRODUCTION 1 1 WHAT IS ATP 1 2 How DOES THE LUMINOMETER WORK 1 3 WHAT IS AN RLU 1 4 TEST DEVICES AND ACCESSORIES LUMINOMETER DESCRIPTION 2 1 KEYPAD SYMBOLS 2 2 INSTALLING BATTERIES 2 3 TURNING THE LUMINOMETER ON AND OFF 2 4 DISPLAY LAYOUT AND ICONS 2 5 Low BATTERY INDICATOR 2 6 POWER SAVING STANDBY MODE 2 7 INTERNAL CALIBRATION 2 8 Low MEMORY WARNING SET UP MENU OPTIONS 3 1 USER ID 3 2 SETTING UP PROGRAM TEST POINTS AND PASS FAIL LIMITS 3 3 TEST PLANS 3 4 ESTABLISHING ATP PASS FAIL LIMITS 3 5 VIEWING STORED TEST RESULTS 3 6 VIEWING STATISTICAL DATA AND TEST FAILURES 3 7 ERASING THE TEST RESULTS MEMORY 3 8 SETTING THE CLOCK TIME AND DATE 3 9 ADJUSTING THE LCD CONTRAST 3 10 QUICK BOOT 3 11 STANDBY MODE 3 12 BOLD TEXT 3 13 SAVE TESTS SAMPLE MEASUREMENTS AND TEST RESULTS 4 1 TAKING A SAMPLE MEASUREMENT 4 2 PERFORMING A RETEST INSTALLING SURETREND DATA ANALYSIS SOFTWARE 5 1 CONDITIONS OF USE 5 2 HARDWARE AND SOFTWARE REQUIREMENTS 5 3 STARTING THE INSTALL PROCESS IT WwW NNN CO o N NOU A R O 10 10 11 12 12 12 13 13 14 14 14
36. y will go blank NOTE To avoid accidental turn off the button is disabled when the luminometer is performing a sample measurement 2 4 Display Layout and Icons DU Y IX ru The liquid crystal display LCD has the following HERE layout The upper half of the display contains the status icons and the large RLU result digits The following is a list of icons and their definitions ww Low battery warning icon Lid icon flashes when the lid needs to be closed or the RS232 connector cover needs to be properly tucked under the lid A Insert icon arrow flashes when the testing device should be inserted T Remove icon arrow flashes when a testing device needs to be removed Jf Pass result icon Caution result icon X Fail result icon HARE Sample measurement reading in Relative Light Units RLU Retested icon identifies the failed test result as having been retested see section 4 2 Q Retest result icon identifies the Program location or test result as a retest result 2 5 Low Battery Indicator The low battery _ icon indicates the state of the batteries Icon Battery State Not visible Good Visible Low replace batteries soon Flashing Flat replace batteries now When the batteries are completely low the luminometer will flash the _ icon beep three times and automatically turn off A CAUTION Never leave low batteries in the luminometer as they are prone to leaking corrosion w
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