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DS Camera Control Unit DS-L2 DS Camera

1. 120 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network e Check the Enable option for Run ActiveX controls and plug ins e Check the Enable option for Script ActiveX controls marked safe for scripting Welcome Microsoft Internet Explorer File Edit view Favorites Tools Help Q sack amp x a A x Search 5 Favorites EA 2 I E 3 Address http 128 31 30 36 r Internet Options General Security Privacy Content Connections Programs Advanced Security Settings Select a Web content zone to specify its security settings d Settings D o J Run Activex controls and plug ins Internet Local intranet Trusted sites Restricted Administrator approved sites Disable Enable Internet Prompt j un xdi contains al weh stes uou J Script ActiveX controls marked safe For scripting haven t placed in other zones Disable Enable Prompt E Downloads 23 Automatic prompting for File downloads Custom settings Disable To change the settings click Custom Level To use the recommended settings click Default Level O Enable v a ot ee eee ee lt gt Security level for this zone Custom Reset custom settings Custom Level Default Level Reset to Medium v 3 Disable personal firewall blocking Some operating systems or antivirus software such as Virus B
2. Back gt j search GyFolders 3 4x GS X A EE Address LEJ My Computer z ei 314 Floppy A JLocal Disk C JLocal Disk D My Computer amp 9Local Disk E amp 3Local Disk F Removable Disk I amp Compact Disc G Removable Disk Removable Disk H Removable Disk I Capacity 60 8 MB amp JUser2 on Psgserv N SxJPsgdata on Psgserv T C Used 22 6 MB 39 Control Panel C Free 38 2 MB 1 object s selected My Computer The drive names of the removable disks differ with each PC environment NOTE In the following cases there might be a conflict between another connected 1 2 3 4 device and the drive letter When media is inserted correctly into position the message Please insert media is displayed when you click the icon for the media drive A similar message is displayed when you click the icon for the CCD image drive Connecting the DS L2 will immediately disable all other USB devices connected to the PC In such a case change the drive letter assigned to the media drive or the CCD image drive The following shows how to make this change Select Start Control Panel Administrative Tools Computer Management Disk Management Right click the volume whose drive letter you want to change and select Change the drive letter and path Click Edit and choose one of unassigned drive letters from t
3. cccccceeccccsseeeeseeeeeeceeeeeeeeeeeesaeeeeesaeeeesseneeeeaees 102 4 2 2 Setting Essential Items Network Settings ccccccecseeeeeeeeeeeeeeeeeeeeeeeens 103 4 3 Operating the DS L2 from a WEB Browset ccccccsseeeeeeeeeeeeseeeeeeeeeeeenaaeees 106 4 5 1 Setting ihe WEB BIOWSSF uu nile eA ocean cenit nel iai 106 4 3 1 1 When Using Internet Explorer ccccccccccecceeeeeeeeeeeeeeeeeeaeeeeesaeeeesaeaeeesaees 106 4 3 1 2 When Using Netscape sssessseeeenneeeennmmem nnne 108 4 3 2 Using WEB Screens cccccccccsssececseseecceeseecceseeccaeeeceuseesseaeeessageeessaneesseass 110 43 9 PICUVEX Settings 55 ioo tusceadiot etra fonte oett cima nte epa s deut edet see a 120 4 3 4 Java Applet Setlingsu su og ote oe ee Sp EL bees Ae we 122 4 4 Using FTP Commands to Operate the DS L2 eeeeeeeeeeeeees 137 4 4 1 Using FTP Commands to Get DS L2 Image Files from a PC 137 4 5 Using Telnet Commands to Operate on the DS L2 sssesssss 138 CONTENTS 4 6 Saving Images to an FTP Server PC from the DS L2 ssessss 139 4 6 1 Setting the FTP Server Functions of the PC esssseeeeeeeeeere 139 4 6 1 1 When Using Windows 2000 Professional XP Professional and Vista 139 2 6 1 2 When Using Mac S J iso I estt med ede EU PR Ce URP ee eS 142 4 6 2 Setting External Serv
4. ccccccccseecceeeeaeeeeeeeseeeeaeeeaes 82 3 4 4 Measuring Perpendicular DistancCe ccccccccccseeeeseeeeceeeeseeeeeeeeeseeseeeeeesaaees 84 9455 WMeSSurtigJXndalessiiisdsend a a a a 85 346 Measuring Crelle Sonen r a a 86 3 4 7 Measuring the Area TR 87 3 4 8 Measuring the Distance between the Center Points of Two Circles 88 349 Measuring the teli teueveei on pudet ebat nce co set ua aE oen cu PEE ufui ou po vo EE sac deadbeat M A ME TRE 89 3 5 Adjusting the Subject Position and Comparing Sizes 91 3 5 1 Using Crosshairs to Adjust the Subject Position eeeessssuss 91 3 5 2 Using Grid Lines to Adjust the Subject Position suuesssuuse 93 3 5 3 Using a Scale to Measure the Subject Size sseeseseeseeeeee 95 3 5 4 Using XY Scales to Measure the Dimensions of the Subject 97 3 5 5 Measuring Vertical and Horizontal Distances eeeeeeseeeeeeeeeee 98 3 5 6 Comparing a Translucent Image to a Live Image eeesesssssess 99 Using the DS E2 via a Network 2er ir oe gears evectus eo vec et Uu ute re dog us Mu De 100 4 1 Typical Method of USO soni meti rte te Ia testi nate ume aes imet hacac seaauace saat 100 4 2 Network Settings nennen nnne nnne nnne nnn nnns 102 4 2 1 Items Needing Confirmation
5. 62 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images 2 1 5 Special Photography on Advn Menu Stroboscope Photographing When you photograph an image with synchronized lighting using a stroboscope plugged into the EXTERNAL IO connector observe the following precautions as you set up the DS L2 e Select ON for External Out in Additional Settings on the SETUP menu and press SAVE This setting enables output of a synchronizing signal e The stroboscope is synchronized when the shutter speed of the DS L2 is in the range of 1 15s to 1s The stroboscope operation is available in DS 5M and DS Fi1 only when the mode is 1 3Mp mode or C Scan mode Select MANU for exposure mode and set the shutter speed within the above range e No noise reduction functions are supported Select NR OFF and leave it selected while in use e The DS L2 does not have an automatic exposure function for stroboscope Use the automatical brightness adjustment function of a stroboscope or select manual exposure in the DS L2 settings e Adjust the white balance from a frozen image following the procedure described below 1 While photographing a uniformly white subject press the freeze button to freeze the display At this time the stroboscope will flash Be careful to avoid overexposure 2 Pressthe button then deselect freeze e The stroboscope emits light when you press the CAPTURE button to save an image or the freeze
6. Windows Messenger Diagnose Connection Problems Sun Java Console Internet Options 2 Select Security Local intranet Custom Level Perform the settings of ActiveX controls and plug ins e Check the Enable option for Automatic prompting for ActiveX controls e Check the Enable option for Download signed ActiveX controls Welcome Microsoft Internet Explorer File Edit wiew Favorites Tools Help Q peck 2 x a A Search ST Favorites 2 B eS 3 Address 48 http 128 31 30 36 Internet Options General Security Privacy Content Connections Programs Advanced Security Settings Select a Web content zone to specify its security settings p Settings 4 o iJ ActiveX controls and plug ins Internet Localintranet Trusted sites Restricted E Automatic prompting For Activex controls sites Disable Enable Internet 9 Binary and script behaviors 4 This zone contains all Web sites you Cha Administrat v d D haven t placed in other zones z ied cee eee Enable Security level for this zone 9 Download signed ActiveX controls Disable Custom Enable Custom settings Prompt To change the settings click Custom Level To use the recommended settings click Default Level i Download unsigned ActiveX controls FM Minella s Reset custom settings Custom Level Default Level Reset to Medium
7. Confirm the effective level of the photographed image from image distribution with a histogram The DS L2 calculates brightness distribution of the image and displays it as a histogram HISTOGRAM This function is performed on the Adv2 menu lis Liss UIE This histogram indicates the distribution of brightness of each R G and B color and can be used for fine adjustment of the exposure level e The horizontal axis indicates the color brightness increasingly darker toward the left and brighter toward the right e The vertical axis indicates the frequency of occurrence which is normalized by the maximum value e The average histogram values of each color are shown in numeric form at the right edge and shown by levels at the bottom 59 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images How to read and set exposure levels If the brightness distribution is concentrated on the right edge of the histogram it means that the highlighted parts of the screen are saturated Conversely if the brightness distribution concentrates on the left edge of the histogram it means that the low lighted parts of the screen are blacked out Although it is ideal if no parts on the screen are saturated or blacked out exposure may have to be adjusted depending on the subject If an area of interest on the screen is highlighted adjust the exposure so that the distribution will be shifted toward the left If an area of inte
8. Press exposure start button and the DS L2 starts automatic exposure When finished a screen is displayed with a niet frozen image F T py ul e is displayed when exposure time is longer than 6 seconds D me n off Settings Right click stops photographing Focus mi If necessary adjust the shutter speed and camera gain manually and press KE exposure start button again The standard tone setting is TONE 3 but you can adjust it to your liking after the exposure Also the brightness can be fine adjusted by icon If the black level is found to stand out after photographing adjust the black level to the negative side with the SFT icon on the Adv2 menu to prevent the black level from standing out When the cooled camera is connected Diecisvetown dr endoi senos is displayed when exposure stops Click on only when the cooled camera is SFT which automatically calculates the amount of connected black level shift for negative side adjustment The amount adjusted is displayed in SFT 61 CAMERA Chapter 6 Basic Operation Method 2 Photographing Images 10 Check the screen for any abnormality If no problems are found save the image Use Capt button REC menu or shortcut menu to save the image For high picture quality set the source to Full with the button of the REC menu 11 To cancel the DF FL scene mode select other scene mode or custom mode NOTE NOTE NOTE When connecting DS L2 with
9. 21 22 23 24 25 31 32 33 34 35 36 37 40 Chapter 6 Basic Operation Method 1 Preparations Before Photographing Other scene 2 Asbestos blue 18 Scene 4 for industrial use Flat panel Scene 1 for biological use Dark field view fluorescence Scene 2 for biological use Bright field Scene 3 for biological use Differential interference phase difference Scene 4 for biological use HE staining Scene 5 for biological use Enzyme labeled antibody method User defined scene 1 User defined scene 2 User defined scene 3 User defined scene 4 User defined scene 5 User defined scene 6 User defined scene 7 Exposure compensation brighten darken E UP SETUP S EIU P Chapter 6 Basic Operation Method 1 Preparations Before Photographing 1 3 4 Status Bar CAH VIEW REC RS TOOL SETUP Eolo v e Advi Adv REG gt gt PROG EEEUEREE 1 ow ERN we Ae cAn v100 1006100 NITE 05 Mr SFT TON SD orr W orr sHP 2 COLOR LSet et e2 e3 cd 5 cb c7 E er SATIS GS U Turn Off settings Focus M 0084 Cursor position Buttons to show or hide menus Niko Emmm P 1 30 5124325 MITIS EAT Exposure information level meter mode exposure time On the status bar are displayed exposure information date and cursor position You can also set display ON OFF of each menu on the status bar The status bar is in a hidden state immediately after the DS L2 is started up When yo
10. New User and enter the User ID and Password of the external FTP server set in the network settings in the Setup menu of DS L2 3 Close the window 4 For the IP address on the server side you can select the built in Ethernet through Apple Logo System Environment Settings Network Settings On home directory settings and the separation of save directories for each of multiple users DS L2s When a user is added a home directory is automatically created under the specified user name and directory name at a location below Macintosh HD Users When image files are transferred the FTP Dir specified on the DS L2 side is created at a position below this home directory and the image files are saved in that directory Example If the user name is DS1 and the FTP folder specified on the DS L2 side is FTPIBOX then the FTP transfer directory is C Users DS1 FTPIBOX When accounts are added separate directories are created for each user so that even if there are multiple users DS L2s connected to the network image files are saved in each separate directory Access rights for the created folder are set so that only the user who created it can access it with both read and write privileges other users may access the folder only to read files Do not change the access rights for the created folder Doing so may make it impossible to transfer images to the folder or result in other users inadvertently d
11. See Chapter 5 Microscope Adjustment Use an appropriate filter When using a phase contrast microscope or an interference microscope two luminous fluxes or multiple luminous fluxes insert the specified filter With a black and white image contrast is improved by inserting a green interference filter GIF Contrast in color images is enhanced by inserting a filter having a complementary color to that of the specimen See Chapter 5 Microscope Adjustment Adjust the ambient light around the microscope Make the room darker or place caps on the binocular eyepiece for example Check the contrast of the specimen itself Adjust the clock phase of the LCD monitor or projector See Section 1 4 6 Adjustment for Using an External Monitor 206 Chapter 8 Troubleshooting 3 Photographing and Saving 3 Photographing and Saving Symptom Check points Captured images have low e Set the image quality in the REC menu Reduce the JPEG Quality resolution and the overlay compression rate or select No compression BMP TIFF information cannot be See Section 7 Operation Differences among DS read Camera Head Models in Chapter 6 Photographing is mistimed Be sure to select a combination of camera mode and source that will not even when freezing cause photographs to become mistimed i e with high snapshot performance See Section 2 Photographing Images in Chapter 6 and Section 7 Opera
12. See Section 4 2 Network Settings in Chapter 6 Check points Follow the sequence shown below to check network settings Check network cable connections cables and hub Y Check the set values DS L2 IP address and PC network settings Y Check the presence on a LAN ping from the PC Y Check browser settings Check FTP settings Checking network cable connections Checking types of cables Check that the twisted pair cables category 5 or 5e for 10 100BASE TX are used If the DS L2 is connected to a LAN via a hub check that straight cables are used If the DS L2 is connected directly to a PC without going through a hub check that crossed cables are used Checking hub connections See the instruction manual supplied with the hub for details and follow the description written in it Do not connect the DS L2 to the Uplink Be aware that Uplink on some hubs is indicated as MDI Uplink 1 x 1 1 x or 8 8 x Also if a hub has cross and straight ports caution must be exercised There are a limited number of useful ports on a hub Be careful when connecting to an empty port because doing so could stop the network after the hub Checking the set value Settings on the DS L2 gt Make network settings in Network Settings of the DS L2 SETUP menu Enable Network IP Address Always be sure to make gateway settings if data communication between different ne
13. with Windows P Tell me why this testing i important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has Click the Continue button passed Windows Logo testing i Continue Anyway STOP Installation 24 SRT RTE Specify C Program Files Nikon Shared Drivers DS L2 for the Copy From box And then click the OK button The file DsCamllsb sys on Unknown is needed Type the path where the file is located and then click OK 2 Click the OK button l 1 Specify the folder Copy files from C Program Files Nikon Shared DriverssDS L2 Found New Hardware Wizard Click the Finish button Completing the Found New Hardware Wizard The wizard has finished installing the software for 339 Nikon USB Eclipse i Microscope Click here Click Finish to close the wizard 179 USB Chapter 6 Basic Operation Method 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function 4 Check that the driver is installed Check that the driver is installed without any troubles Operation procedure My Computer menu Explore Search Manage Map Network Drive Disconnect Wetwork Drive Create Shortcut Delete Explorer
14. 2 Click Next What do you want the wizard to do Found New Hardware Wizard Please choose your search and installation options eS Click the Don t detect will select it from a list And SS then click Next Search for the best driver in these locations Use the check boxes below to limit or expand the default search which includes local paths and removable media The best driver found will be installed Search removable media floppy CD ROM 1 Click here Include this location in the search zNProgrem Files Nikon Shared Drivers DSU Browse 2 Click Next Don t search will choose the driver to install Choose this option to select the device driver from a list Windows does not guarantee that the driver you choose will be the best match for your hardware Cancel 177 US B Chapter 6 Basic Operation Method 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function Found New Hardware Wizard Click the Have Disk button Select the device driver you want to install for this hardware C Select the manufacturer and model of your hardware device and then click Next If you have a disk that contains the driver you want to install click Have Disk Show compatible hardware Model Click the Have Disc button A This driver is not digitally signed Tell me why driver signing is important Screen for specifying file location Specify C Program Files Nik
15. General Appearance Bookmarks Tabs RSS AutoFill Security Advanced Web Content M Enable plug ins vi Enable Java M Enable JavaScript Block pop up windows Accept Cookies O Always Never Only from sites you navigate to For example not from advertisers on those sites Show Cookies IV Ask before sending a non secure form to a secure website Enable parental controls 2 Disable personal firewall blocking For details refer to Section 4 3 3 ActiveX Settings For MacOS and Firefox 1 Change the browser settings e Select Firefox gt Preferences r Joia File Edit View Go Bookmarks Tools Window Help About Mozilla Firefox Preferences Services Hide Firefox Hide Others Show All Quit Firefox e Click Content If the Content icon is not displayed click the button in the upper right corner e Select Enable Java e Select Enable JavaScript 135 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network BAC Content ri al Lat C General Privacy Content Tabs Downloads Advanced Block Popup Windows Allowed Sites vi Warn me when web sites try to install extensions or themes Exceptions PF i o _ for the originating web site only Enable Java Enable JavaScript Advanced Fonts amp Colors Default Font Times Pa Size 16 Advanced J Colors 7 2 Disable personal firewall blocking e For details re
16. S Qu http plueins netscape com meeeinfo dp PID t 0047 a browser plug in Sound complicated It s not The Netscape Plug in Finder has already done most of the work for you Below is a list of plug ins that will enable you to view the information on the page Click the Download button for the plug in you would like to use To learn more about Netscape plug ins click here VA iiil Java by Sun Microsystems Available for Wndows95 Wndows98 WndowsNT WndowsME WndowsXP Search for Plugins supporting All Platforms All File extensions All MIME types All Languages 132 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network e After the Java download page is displayed click Download next to the Windows file The page appearance may vary In this example you can choose either Online or Offline Download Java software from Sun Microsystems Netscape a File Edit view Go Bookmarks Tools Window Help Q Q Q Q http www java com en download manual jsp v ex NC Welcome to java com Brought to you by Sun Mic seach Duke s Zone Games Mobile Desktop Java Everywhere JAVA SOFTWARE Java Runtime Environment Version 5 0 Update 9 To complete the download of the Java Runtime Environment please select from the list below Once you have Java software you ll have access to a whole new vvorld of interactivity Please note that downloads
17. 281 2535 X4 7F EX d BEM T460 0002 Aw PR AOA 3 20 17 BP ERRORS E E JU Biz 052 954 0165 X4 7F EX NIKON CORPORATION 2 3 Marunouchi 3 chome Chiyoda ku Tokyo 100 8331 Japan Sales Dept Bio Science Division Instruments Company Parale Mitsui Bldg 8 Higashida cho Kawasaki ku Kawasaki Kanagawa 210 0005 Japan Tel 81 44 223 2167 Fax 81 44 223 2182 Sales Dept Industrial Instruments Division Instruments Company Parale Mitsui Bldg 8 Higashida cho Kawasaki ku Kawasaki Kanagawa 210 0005 Japan Tel 81 44 223 2175 Fax 81 44 223 2182 NIKON INSTRUMENTS INC 1300 Walt Whitman Road Melville NY 11747 3064 U S A Tel 1 631 547 8500 Fax 1 631 547 0306 Semiconductor Inspection Technologies Division 1430 W Auto Drive Suite 101 Tempe AZ 85284 U S A Tel 1 480 403 4100 Fax 1 480 403 4199 NIKON INSTRUMENTS EUROPE B V Schipholweg 321 P O Box 222 1170 AE Badhoevedorp The Netherlands Tel 31 20 44 96 222 Fax 31 20 44 96 298
18. 3 Adjust the focus Adjust the image composition and the focus 4 Compensate the exposure ue Adjust the brightness of images with the exposure compensation button md 5 Save the image Save the image with gt G Medium save button 45 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images 1 Selecting the scene mode or the custom mode Before photographing select either of the mode according to the subject and observation method The custom mode enables to read out the photographing conditions saved previously Select the scene mode or the custom mode on the Adv1 Advanced1 menu of the CAM menu To disply the Adv1 menu diplay the main menu and select the CAM tag The CAM menu appears And then select the Adv1 tab At the lower of the Adv1 menu the scene mode and the custom mode are shown Scene mode The scene mode enables you to adjust the gradation and color of images according to the subject To change the scene mode for industrial or biological specimens select either of the following modes on the Cam OSD of the Power On Settings as selected from the SETUP menu e Industrial specimen scene mode Ind e Biological specimen scene mode Bio e Other scene mode etc e Industrial specimen scene modes Wafer IC chip Suitable for wafer or IC chip Metal Ceramic Bright parts of a metal specimen are expressed as white and dark parts are expressed as transparent This mode can
19. 543 Using the Media Drive iei tota to etaua aves aou cS e poa repa e tola ci a soror o gab eie Eo Ero oka sEd 161 5 4 4 Using the CCD Image Drive ccccccssecccceeeeeceeeeeceeeeecsegeeesseseeesaseesseneeeses 162 5 4 5 Removing the DS L2 from the PC seeeesssessseeeeeeeeeenn nennen 164 5 5 Using the DS L2 in Windows Vista eesssssessseeeeeeeenn enn 165 5 5 1 Installing the Device Driver seeeseeeeeeeeerneeennnn nnn 165 5 5 2 Outline of the Disk Drive ccccccecccccsececceeeeecseeeeeceeesecseeeesseseeesseesessneeeses 166 5 5 9 Using INE Media DIVE un cii terea denne eue cota coeno cea drole cde vapeur ara a ia cda 167 554 Using the CCD Image Drive wee sisse cheek etes tque i usos 168 5 5 5 Removing the DS LE2 from the PG ie e DR Heer noe et ec el tese ee Ob ades 170 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function 171 6 1 Installing Application Software seeeseseesseeeneeeenennnn 171 02 Instaliiag Device DIVET cationic e rhet acia tt aetate adorti ie 173 Oo 1 6 geet rem I Uu e 182 7 Operation Differences among DS Camera Head Models cccccceeceeeeeeeeeeeeeeeeaneeees 184 MES Ium E 184 Tok DS QIME pm 187 CBS css ots ea ese sce 193 7 4 DS 2Mv DS 2MBW DS 2MBWG eesssesseeeennennn nennen nnnm hannes nnn ne sante aane s sen 195 7
20. 800x600 Fine v Quality Capture 1600x1200 Fine Exposure Mode Manual Exposure v AE Compensation l l 1 90 s X Fps 1 71 11 5 ms 1 7 ee 55 Uncalibrated N Mono 8bit 800 x 600 pixels 3 Documents treatment Opens an image file stored on hard disk This command can save a captured or modified picture on hard disk Opens a printing dialog window that enables you to print the current image if a printer is present This button creates a basic image report It makes a pdf file that contains the current image its name path dimensions and the file date 1 Operation modes k Point Q oom A Annotate This mode enables you to click on the image and move it within the image window Zooming can be performed by a mouse wheel This mode changes the cursor to an interactive zoom tool that magnifies a circular part of the current image when clicked This mode enables you to insert annotation objects into pictures according to the Tools settings 2 Areas of interest Focus This tool defines the area of the live image that is used to determine auto exposure parameters applied to the camera This tool defines the area of the live image that is used to determine the auto focus parameters Place the rectangle to where you would like to focus 4 Zooming EZ Fil 1 Original oom Out en c ed 182 Adjusts the zoom factor to view
21. Chapter Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu 6 If you want to attach information to the calibration value you have created click the Info input box and enter text for the information you want to attach This information will prove helpful because you can confirm Info when you call up the calibration value at a later time 7 Check the Set number to see if the calibration number is correct and press to save Unless you enter a numeric value and a unit you cannot press the button If you want to recalibrate use to erase the current calibration value before saving it 8 Press to return to the previous menu Any calibration values will be erased unless they have been saved NOTE Do not save calibration values while you select E on the calibration screen If so wrong calibration values are saved lt When using the magnification of the objective and relay lenses gt 1 Select a number in which you want to save the calibration value from through m7 The selection can be made in the calibration menu as well Drag the mouse pointer over one of numbers through m7 and the calibration information for that number will be displayed in the Info area for your confirmation NOTE If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope with motorized or intelligent nosepiece note that each calibration value
22. Exceptions column write the IP address of the DS L2 Refer to Section 4 2 2 Setting Essential Items Network Settings in Chapter 6 for details on the IP address of the DS L2 e Enter a period after every three digits of the IP address e If the first digits of the IP address are OO or 0 do not enter the zeros Proxy Settings m Servers ml Type Proxy address to use Port isl LEM gy Secure 1 Gopher O Ha Socks e Lo o Use the same proxy server for all protocols Exceptions Mis Do not use proxy serverfor addresses beginning with P Enter the IP address here Example When entering the IP address 192 168 1 2 Use semicolons to separate entries omen _ 8 Click OK Move the pointer T to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 107 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 4 3 1 2 When Using Netscape 1 Start the PC Refer to your computer s user manual for details 2 Launch Netscape Refer to your computer s user manual or the Netscape help function for details 3 Select Edit Preferences Left click here to open the pull down menu and select Preferences from the menu Gut Greed a vl E Zs A Home Search Netscape Print Security Shop Ga what s Related Girj j CEE
23. File Edit WEWE Favorites Tools Help Toolbars Status Bar Explorer Bar Filmstrip Thumbnails Tiles Imaiza0 Icons List Details Arrange Icons by Choose Details i30 To Refresh c2 Make a new Folder EE Publish this Folder ka Ehe Web E Share this folder Other Places See MIEONIMG 3 7 ELI m 2 Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed In DS L 21MG File Edit View Favorites Tools Help Q9 pack d Jj search Folders Address O JADS L21MG Preview Edit Print Picture Tasks m View as a slide show S Order prints online E Print this picture he Set as desktop background g amp Copy to CD Open With PC cillin Send To Cut Copy File and Folder Tasks m Create Shortcut mi Rename this file Delete iy Move this File Rename Copy this File g Publish this file to the Web E mail this File Delete this file Properties 163 USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 4 5 Removing the DS L2 from the PC Because the DS L2 is connected via a USB interface it can be removed from the PC even while the PC remains powered on However to prevent data from being corrupted while removing the DS L2 during a write operation follow the procedure described below
24. Installing device driver software Click here for status JM Your devices are ready to use Device driver software installed successfully 165 US B Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 5 2 Outline of the Disk Drive When the DS L2 is connected to a PC the disk drive for the CF card etc and the internal image drive of the DS L2 are each recognized as one removable disk by the PC If the DS L2 is already connected to the PC two removable disks are listed under Computers on the PC s desktop Organize vy Views system properties wi Uninstall or change a program Map network drive gt g m prop m ge a prog p Name Total Size Free Space Hard Disk Drives 2 Local Disk C Local Disk D gt E Documents E Pictures lE Music Sy 46 9 GB free of 58 5 GE CU 00 3 GB free of 904 GB 4 Recently Changed Devices with Removable Storage 5 BY Searches 4 il Floppy Disk Drive A DVD RW Drive E d Public Sr Ba 3 Removable Disk F NIKONIMG 6G z Removable Disk H BS4 PC VISTAE Workgroup S INF PS A Processor Intel R Pentium R 4 CPU 3 40GHz p hts 2 00 GB NOTE In the following cases there might be a conflict between another connected device and the drive letter e When media is inserted correctly into position the message Please insert media is display
25. Preferences Category Appearance X Mavigator Features that help interpret web pages History Languages Enable Java Helper Applications Enable native object scripting Smart Browsing _ Send this email address as anonymous FTP password Piden p sg Tabbed Browsing Downloads b Composer Quick Launch gt Mail amp Newsgroups b Instant Messenger O Keep Netscape in memory to improve startup performance b ITQ Privacy amp Security IF vau check this item part of Netscape will stay in memory when nat in use allowing it to start up Faster k Advanced Offline amp Disk Space ok j cae Heb 130 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network e After selecting Category gt Advanced gt Scripts amp Plugins select the option to enable JavaScript for Navigator Preferences Category EE b Appearance b Navigator Enable JavaScript Far Composer gt Mail amp Newsgroups Navigator gt Instant Messenger Mail amp Newsgroups b ICQ b Privacy amp Security Allow scripts to s Advanced Move or resize existing windows Scripts amp Plugins Raise or lower windows Keyboard Navigat Hide the status bar buzarhe Change status bar text pines Change images HTTP Networking Create or change cookies Software Installat Readcenkies Mouse Wheel System Offline amp Disk Space Enable Plugins For Mail amp Newsgroups 2 Instal
26. User ID anonymous max 10 char Pass Word none max 10 char Enables disables network Enable FIP Client n IP Authority operation External Server Paddress 192 168 020 001 ping test Server No 1 User ID anonymous max 10 char the DS L2 Pass Word none max 10 char Bate way 000 000 000 000 login test E Save Default MAC Address 00 90 B5 43 5E 39 skip 0 1 Za S alte oe BS eg 9 asse ESSET Eg BS S S 1 om n o p q n S t u V W X yez Enable FTP Client For image files to be sent to the FTP server always select this check box to enable the FTP client function External Server Set the IP address and the gateway address of the server PC Before images can be sent to any FTP server that belongs to a different network group you must enter the gateway settings Server No Five servers can be stored in memory Assign each server a number IP Address Input example User ID Enter a user ID that is needed to log in to the server Pass Word Enter a password that is needed to log in to the server as necessary Gate Way Input example If any entry in the IP address consists of two digits or one digit add 0 or OO at the beginning of it to make each entry consist of three digits as you enter Click the input box and enter an address value If you missed entering any address value hold down on the screen keyboard while you skip to the last entry of the address then e
27. and select the REG2 menu to change the contents of CCD Mode 2 and CCD Mode 4 CROP Arpa CCD Mode 4 3 12864488 CROP Arpa CCD Mode 4 3 12864968 Einning t CCD Mode 2 5 ax x x4 Einning CCD Mode 2 3 Bx 3x4 C Scan 1280 480 is selected ROI 1280 960 is selected for CCD Mode 4 for CCD Mode 4 1 3Mi X2 is selected 640 X4 is selected for CCD Mode 2 for CCD Mode 2 185 CAM E RA VARI ETY Chapter 6 Basic Operation Method 7 Operation Differences among DS Camera Head Models Size of saved images Select the size of saved images by doing CCD source setting and setting of size of saved images on the REC menu CCD source setting represents the CCD mode during image acquisition when you freeze save or print images When Source Full is selected the CCD mode is automatically switched to 5Mi CCD Mode 3 during image acquisition and images with high resolution can be obtained Note that switching CCD modes will result in a brief delay When Source Now is selected images are acquired in the current CCD display mode This setting in which the CCD mode is not switched is recommended when you expect to freeze images repeatedly Counpeat Now CCD source settin 25EDx1992n Setting of size of saved images When the CCD source setting is Source Now and the CCD mode is 1 3Mp or 1 3Mi select 1280 960 or 640 480 for the setting of s
28. button to freeze the display Freeze the display before saving an image this will allow you to check the photographic conditions NOTE Compared to ordinary photography there is a shutter delay in strobe photography because the stroboscope emits light only after you press the CAPTURE button 63 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images 2 1 6 Registering Items Relating to Camera Photometry REG1 Menu You can register the photometry area shading tone and camera settings on this menu 1 Registering the photometry area Use the photometry area button on Adv1 to set the photometry area After the photometry area is set the a1 to a3 buttons become available Select values you wish to register and press to complete the registration Refer to Section 2 1 3 Fine Adjustment before Photographing Advn Menu 4 Choosing photometry mode Selecting a photometry area in Chapter 6 for details on how to set the photometry area AE Area al a a3 2 Calculating and registering the shading Shading on DAL Perform shading correction for illumination and optical units Do so 1 s2 sa 54 sf without any shielding objects 1 s2 s3 s4 sb st s7 TONE When you press SD CAL shading calculation is started After the calculation data is obtained the s1 to s7 buttons become available Select values you wish to register and press to complete Camera Settings the registration sf ctf ce cf
29. so that files will be named after the photographed date and time 1 2 4 5 Press The Cnt and Int icons will be displayed In Cnt set the number of frames to be photographed Up to 500 frames can be set If you select the Endless option images are photographed continuously until you press the Stop or until the destination medium CF USB memory or FTP is full In Int set an interval time ranging from 10 seconds to 6 hours Choose the desired interval time from the following 10s 15s 20s 30s 45s 60s 1m30s 2m 3m 4m 5m 6m 10m 15m 20m 30m 45m 60m 1h30m 2h 3h 4h 5h 6h Select the drive and press and interval photography will start with images saved to a USB memory Press to stop photographing in the middle Depending on the length of time required for writing to a medium the DS L2 may not be able to save images at the specified time intervals Prior confirmation is necessary before you begin interval photography If images cannot be saved at specified intervals During normal operation V Photographing V Photographing V Photographing Int set value Int set value Int set value Save Save Save When set intervals cannot be maintained V Photographing V Photographing Int set value Int set value Int set value Save Save 67 CAM E RA Ch
30. the pupil of the objective lens Avoid over enlargement The scaling factor should be set in a range of 500 to 1 000 times the numerical aperture of the objective lens Set the exposure or tone to an appropriate level See Section 2 Photographing Images in Chapter 6 Set the microscope illumination at an appropriate level 205 Symptom The color is poor The contrast is low Noise appears at the edges of the menu when shown on an LCD monitor or projector Chapter 8 Troubleshooting 2 Image Output Check points Set the white balance See Section 2 Photographing Images in Chapter 6 Apply a moderate amount of color compensation See Section 2 Photographing Images in Chapter 6 Turn off hue adjustment See Section 2 Photographing Images in Chapter 6 Set the color of the internal monitor and external monitor to an appropriate level See Section 1 4 6 Adjustment for Using an External Monitor Set the color temperature of the illumination to an appropriate level Set the tone appropriately See Section 2 Photographing Images in Chapter 6 Avoid adjusting the black level to the positive side of SFT See Section 2 Photographing Images in Chapter 6 Adjust the size of the field diaphragm so that it circumscribes the viewfield Adjust the size of the aperture diaphragm to approximately 70 to 80 of the numerical aperture of the objective lens
31. 1 2 3 4 5 Eject the media from the media drive before removing the DS L2 Click the Safe to Remove Hardware icon on the task bar Safely remove USE Mass Storage Device Drive Safely remove USE Mass Storage Device DrivetH Drives should be selected one at a time First select the media drive If the media drive can be removed the message shown below is displayed d LJ Safe To Remove Hardware The LI5B Mass Storage Device device can now be safely removed From Ehe system To remove the CCD image drive follow step 3 Unplug the USB cable or turn off the DS L2 Plug in the USB cable and turn on the DS L2 It will be recognized by the PC again It is recommended to set the date and time information within the file settings such that file names are automatically generated 164 Chapter 6 Basic Operation Method USB 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function EEFT Using the DS L2 in Windows vista 5 5 1 Installing the Device Driver When the DS L2 is connected to a PC for the first time the PC automatically recognizes the DS L2 and installs the device driver that comes standard with the OS During the installation process messages will be displayed sequentially in the task tray system tray at the lower right corner of the monitor screen indicating that new hardware has been detected You do not need to perform any specific operations
32. 1 How the menus are displayed partly varies depending on the model purchased DS Fi1 CAH view REC poama ET CHP BAIN 1 245 5467 ETA T ET 7 Eu CHP BAIN BAIN CHRM 00 HUE SFT Sr I 00 MID 00 ilis SFT Adv1 menu Adv1 menu Adv2 menu 1 3Mi amp C Scan are selected 640 amp ROI are selected Features of display modes 1 3Mp An image area of 1280 960 pixels is formatted for the monitor resolution CCD Mode 1 The image is displayed at actual size 1280 960 SXGA or reduced 1024 768 XGA The frame rate is 12 fps This display mode is useful when good performance with moving subjects is required Progressive display modes eliminate color shifts and provide natural images even with moving subjects We recommend using this display mode as the general display mode 4 3Mi An image area of 1280 960 pixels is formatted for the monitor resolution CCD Mode 2 The frame rate is 10 fps As two pixels are added respectively in the vertical and horizontal directions to make one pixel this mode is four times more sensitive to light than other modes making it particularly useful for dark subjects Interlaced display modes may cause color shifts with moving subjects 640 An image area of 640 480 pixels is formatted for the monitor resolution CCD Mode 2 The frame rate is 16 6 fps As four pixels are added respectively in the vertical and horizontal directions to make one pixel this
33. 1 Press Grid and grid lines will be displayed 2 The color of the grid lines can be selected from six colors red green yellow blue white or black 3 Press the tab and then press on the TOOL menu to bring up the tool shown below This tool allows you to set the space between the grid lines that suits the subject whose position you want to adjust view REC D ule Ea Direct Glick moda input Value mode VIEW REC DE N Direct Glick moda Ed Input value mode K 4 Bj m E 8 EJ Ent ETIE standard Position Ej Left amp Up Side FH Center Saye 4 Select one from Direct Click mode and Input Value mode to set the space between the grid lines Please Click the Foints Min Sp Op ix avp 5 In Direct Click mode click two arbitrary points on the image to set the space Clicking the button allows you to perform the setting beginning with the first point again 6 In Input Value mode input values for the spaces in the X horizontal and Y vertical directions Clicking each value display cell enables the numeric key icons to let you input values Click after you complete the input When Left amp Up Side of Standard Position is selected the upper left side of the image will be a standard position When Center is selected the center of the image will be a standard position 7 Press to save the grid lines settings The color and space of the grid lines you have set in 2 to 6
34. 2MBW or DS 2MBWc the magnification of the objective is 40 times that of the relay lens is 0 55 times and the monitor inch size is 8 4 diagonal size 213 mm e g 2 N e g 3 N Magnification on the monitor 40 x 0 7 x 213 10 57 approx 560 times where the camera head is DS Qi1Mc the magnification of the objective is 40 times that of the relay lens is 0 7 times and the monitor inch sizes is 8 4 diagonal size 213 mm 199 CAM ERA VARI ETY Chapter 6 Basic Operation Method 7 Operation Differences among DS Camera Head Models Calculating the magnification at the time of PictBridge printing Use the following formula to calculate the magnification of a subject on a printed sheet Magnification on the printed sheet Magnification of the objective x Magnification of the relay lens x Diagonal size on the printed sheet Diagonal size on the CCD effective area Calculation examples e g 1 Magnification on the printed sheet 40 x 0 7 x 163 10 88 approx 420 times where the camera head is DS Fi1 DS 5M or DS 5Mc the magnification of the objective is 40 times that of the relay lens is 0 7 times and the sheet size is L size diagonal size 163 mm e g 2 Magnification on the printed sheet 40 x 0 55 x 163 8 8 approx 410 times where the camera head is DS 2Mv DS 2MBW or DS 2MBWc the magnification of the objective is 40 times that of the relay lens is 0 55 times and the sheet size is L
35. 5 Magnification for Observation and Printing ccecceeeseeeeeeeeeeeeeaeeeeeeaeeeeesaaes 199 8 Connecting an LV Series Microscope sssssssssssseeeeeenneee mene nennen nnns 202 Chapter 7 Care and Maintenance cccccccceccccescceeeeeeeececeeeeeeeeeeeeceeeeceseeeesseeeseeceseeeesansessaeetsneseetsneeeas 203 1 Cleaning the SVSEe ITI ones outta EE Roh Cete R3 Ua eue Usb bee estime adea e tinatula c dunt E wage d 203 2 ro clo RNC ENT m PMen 203 Chapters Troubleshooting o e pr xo eot stadia AA sot ia eq ae 204 T Power OUDDIV semen eects ab esac oaakc eects satu Du DENIM ELEC US CA RIDE IIIS 204 2 mage CUL DUL o enira aui occ ee act utn dir cU MNA D D PIA LM IE 205 e MI s Chapter 9 CONTENTS 3 Photographing and SAVING iod expen soto n e otee o bae Bota ho drain sone su ene Udo ce ene tudo ao been ae does icd eus 207 4 Abnormal BehaViOE usui ite ravi a a a Edu E Dan a aea a a 207 9r INGO S assiduis brodo ton iL bi d tubes ue 208 MMMU T T cr eee 211 7 Microscope Control from the DS L2 ssessssssssssssesseeseen enne nnne nennen ns 213 IBEIOre Contacting US Tt 214 Technical Specifications E ET D DLE 215 T DS Camera Conuol Unit DS3 2 3 aH Een pera esa in edes unen in eade ua chien ide eue bdo ona odis ws 215 2 DS Camera Sd cte 6 caine testas ina edsu
36. 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function Confirming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed w DS LZIMG File Edit view Favorites Tools Help Back Toolbars P fides c o OS x o9 Ed wv Status Bar Address E Explorer Bar d P Large Icons Small Icons lal1280 List DS L2 Details Thumbnails IMG1280 JPEG Image Arrange Icons Modified e Lime Up Icons Size 263 Kl Customize This Folder Attributes Go To d Refresh 2 Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed D5 LZIMG File Edit View Favorites Tools Help da Back search L5Foldees GA Dx OS x ux Ez Address D5 L2IMG P Open DS L2IMG Open With Cal Winzip IMG1280 Send T b JFEG Image Sue Modified 8 15 2005 7 07 PM os Copy Size 263 KB Create Shortcut Attributes normal Delete Rename Properties 157 USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 3 5 Removing the DS L2 from the PC Because the DS L2 is connected via a USB interface it can be removed from the PC even if the PC remain
37. EE EN E ve Ec ED 100 100 e100 g SFT TONS Tu bM MINN DS 2Mv Adv2 menu DS 2Mv DS 2MBW DS 2MBWc 2l Bo SJ Boo 34 CSc PROB pu oP E GAIN ME JARE 00 SD OFF NR OFF SHP O B a W ose ev er ea et eol co e7 re DS 2MBW Adv1 menu 73 GAIN MIU DS 2MBW Adv2 menu en Boo SS Boo SS CS ci PROB ajA oP 00 AL GAIN MU em 00 SD OFFINR OFF SHP O B aW ose DS 2MBWc Adv1 menu 73 GAIN MIU DS 2MBWc Adv2 menu When DS 2Mv or DS 2MBW is connected the display that represents the cooled camera is not displayed When DS 2MBW or DS 2MBWcC is connected setting items related to colors are not displayed 195 CAMERA VARIETY 7 Operation Differences among DS Camera Head Models Chapter 6 Basic Operation Method Features of display modes 2MFull CCD Mode 1 800 6 CCD Mode 2 800 5 CCD Mode 3 C Scan CCD Mode 4 An image area of 1600 1200 pixels is formatted for the monitor resolution The image is displayed at actual size 1280 960 SXGA or reduced 1024 768 XGA The frame rate is 15 fps This display mode is useful when viewing images in great detail We also recommend this mode when making long exposures Progressive display modes eliminate color shifts even with moving subjects We recommend using this display mode as the general display mode An image area of 800 600 pixels is enlarged for display at the monitor resolution The fra
38. ETWORK 4 Using the DS L2 via a Network e Click Finish i Java 2 Runtime Environment SE v1 4 2_11 Complete InstallShield Wizard Completed The InstallShield Wizard has successfully installed Java 2 Runtime Environment SE v1 4 2 11 Click Finish to exit the wizard Finish Cancel e fa message requests that you restart your computer close any open programs and click Yes to restart 3 Disable personal firewall blocking For details refer to Section 4 3 3 ActiveX Settings 125 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network For Windows and Firefox 1 Change the browser settings e Select Tools gt Options Mozilla Firefox File Edit view Go Bookmarks BESSI Help Qa T CP v Ej A Web Search Ctrl K Read Mail 0 new _ Customize Links Free Hotmail New Message Ctrl M Downloads Ctrl J Extensions Themes JavaScript Console Page Info Clear Private Data Ctrl Shift Del e On the Content tab select Enable Java e Select Enable JavaScript Options i Aona cC General Privacy Content Tabs Downloads Advanced Block Popup Windows Warn me when web sites try bo install extensions or themes Load Images For the originating web site only Enable Jaya Enable JavaScript Fonts amp Colors Defau Eont xe 16 w Cancel 126 Allowed Sites Exceptions Exceptions Advanced Advanced Colors C
39. Nikon Shared Drivers DS L2 for the Copy manufacturer s files from entry Install From Disk Insert the manufacturer s installation disk and then LA make sure that the correct drive is selected below And then click the OK button 2 Clisk the OK button 1 Specify the folder Copy manufacturer s files from C Program FilessNikon S hared Drivers DSL2 Hardware Update Wizard Select the Nikon USB DS Camera And then click arc the Next button The driver will be installed Select the device driver you want to install for this hardware Na ty 1 Select the Nikon USB DS Camera 2 Click the Next button Select the manufacturer and model of your hardware device and then click Next If vou have a disk that contains the driver you want to install click Have Disk Show compatible hardware Model Nikon USB DS Camera This driver is not digitally signed Tell me why driver signing is important 175 US B Chapter 6 Basic Operation Method 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function Click the Continue button Hardware Installation AN The software you are installing Far this hardware Nikon USB DS Camera has not passed Windows Logo testing to werty its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system eithe
40. Paste Ginty Select AIl Otrl A Eindin Rage HH Bind Agatti Btri teE Search Internet Preferences 4 Select Advanced Proxies Left click Advanced When a menu appears under Advanced left click on Proxies Preferences Category Appearance Fonts Colors 3 Navigator Iv Automatically load images Languages Iv Enable Java Applications wi Smart Browsing Iv Enable JavaScript H Mail amp Newsgroups Iv Enable JavaScript for Mail and News amp Roaming Access H Composer Offline OB Change preferences that affect the entire product Iv Enable style sheets Send email address as anonymous FTP password Cache Proxies Oookies SmartUpdate Accept all cookies C Accept only cookies that get sent back to the originating server C Disable cookies Warn me before accepting a cookie oen tee Left click here 108 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 5 Select the check box labeled Manual Proxy Configuration and then left click on View Preferences Category E Appearance Pr Kies Configure proxies to access the Internet Fonts Colors pee A network proxy is used to provide additional security between your Languages computer and the Internet usually along with a firewall and or to Applications increase performance between
41. Specify the size of saved images When the CCD source setting is Source Now and the CCD display mode is 1 3Mp select 1280 1024 640 512 or 320 250 for the size of saved images When the CCD source setting is Source Now and the CCD display mode is 640 select 640 480 or 320 240 for the size of saved images When the CCD source setting is Source Now and the CCD display mode is 320 select 320 240 for the size of saved images When the CCD source setting is Source Now and the CCD display mode is Hi24 select 1280 720 for the size of saved images When the CCD source setting is Source Now and the CCD display mode is ROI32 select 640 480 or 320 240 for the size of saved images When the CCD source setting is Source Full the CCD mode is automatically switched during image acquisition regardless of the current CCD display mode Thus you can select 1280 1024 640 512 or 320 240 for the size of saved images The following table gives the resulting file sizes for various image file settings Because actual file sizes of JPEG images can vary widely depending on the subject these JPEG values are merely guidelines eee Fine Normal Basic Draft 640 512 960KB 960KB 640 480 900KB 260KB 120KB 80KB 60KB 900KB 320 256 240KB 240KB 320 240 230KB id id us ree 230KB The size of saved images can be set th
42. The frame rate is 46 8 fps Because four pixels are added vertically and horizontally for each pixel created this mode is 16 times more sensitive to light than 1 3Mp mode making it useful for dark subjects Hi24 An image area of 1280 720 pixels corresponding to the center of the image CCD Mode 4 is formatted for the monitor resolution The top and bottom 1 7 are not displayed The frame rate is 24 2 fps This mode is useful if a 16 9 aspect ratio is needed e g HD applications RO 32 An image area of 640 480 pixels corresponding to the center of the image is CCD Mode 4 formatted for the monitor resolution The top bottom left and right 1 4 are not displayed The frame rate is 31 6 fps This mode is useful if high resolution images and good video performance are required 187 CAMERA VARIETY Chapter 6 Basic Operation Method 7 Operation Differences among DS Camera Head Models To switch between functions in CCD Mode 4 select the REG gt gt tab in the CAM menu then select the REG2 menu CROP rea CCD Mode 4 1250720 Ex Trigger Mode Hon aw COD Cooling Temp n s EIE hd EH L m 10 C B ERHmax e When Hi24 1280 720 is selected in CCD Mode 4 Black level shift adjustment CROP rea CCD Mode 4 3 1250720 Ex Trigger Mode Hon aw COD Cooling Temp us M Em hd E E m 10 C 602 RHmax e When ROI32 640 480 is selected in CCD Mode
43. USB cable into the USB port of the PC main unit or the hub 5 Plug the B connector of the USB cable into the USB device connector of the DS L2 If this is the first time you are using the DS L2 in Windows the PC automatically recognizes the DS L2 and installs the device driver Refer to the subsequent pages for details 151 U S B Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 9 3 When the DS L2 is connected to a PC for the first time the PC automatically recognizes the DS L2 and installs the device driver that comes standard with the OS During the installation process the window shown below will be displayed sequentially You do not need to perform any specific operations Found New Hardware Found New Hardware Found New Hardware Found New Hardware gt Found New Hardware Found Mew Hardware E Found Mew Hardware Found New Hardware 152 Chapter Basic Operation Method USB 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 3 2 Outline of the Disk Drive When the DS L2 is connected to a PC the disk drive for the CF card etc and the internal image drive of the DS L2 are each recognized as one removable disk by the PC When the DS L2 is connected to a PC two removable disks are displayed in My Computer on your PC s desktop ojx File Edit view Favorites Tools Help
44. Windows desktop to display the shortcut menu E iuda Select Properties on the shortcut menu to open the nn SA Display Properties dialog box Manage Map Network Drive Disconnect Network Drive 1 Right click on the desktop Create Shortcut Delete Explorer 2 Select Properties Properties Display Properties dialog box Click the Screen Saver tab in the Display Properties Display Properties dialog box Px Themes Desktop Screen Saver Appearance Settings Click the Screen Saver S text box and select None from the list then click the OK button Screen Saver tab Screen saver 3D FlowerB os ame eisplau Welcome screen 3D Flying Objects Click None 3D Pipes power settings and save energy Power 173 US B Chapter 6 Basic Operation Method 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function 2 Install the camera driver Connect the camera and then install the camera driver Operation procedure Connecting the USB connector USB A connector J Found New Hardware X D5 L2 Welcome to the Hardware Update Wizard This wizard helps you install software for Nikon USB DS Camera If your hardware came with an installation CD lt 6 or floppy disk insert it now What do you want the wizard to do oftware automatically Recommended Install from a list or specific location Advanced Click Ne
45. a location where it will not be subject to vibrations or excessive amounts of dust Avoid storing the equipment in hot humid locations CONTENTS Table of Contents Salo MENSIS E 1 4 Tyoesor DS Camera Hedd eti ttr In ln ta o enun imet aee hese QinDbes due t ier une maU er S AdUdS 1 EG enieei ciM T TTD 2 Chapter 2 Peripheral Equipment su abu Lodo lol dened erbe buius iad allah dec Uo cu des dicor todas usc dul eo ote udi 3 Chapter 3 Names of Parts and Their Functions esseessssssssssssssesneeeenenen nene nennen nnn nnns nenas 7 1 DS Camera Control Unit DS L2 sssssssessssssesseeeeee nennen nennen nnne nnne nnns 7 2 DS Camera TA CA ERE mw 10 3 DS Remote Controller DS RC Optional cc ccccccccecseeeeeeeeeeeeeeeeeeseeeeeeseeeeesaeeeeeseeeeeeas 10 Chapter 4 Connecting and Installing the EQuipMent ccccccccceeccceeeeeceececeeeeseeeeseeeeeseeessueeseeeeseeeeseaes 11 UNES re ain Teile d BI o IRR EDI DILE 11 2 stalling Ne DS L2 4 tette eu ER ER Spon anto iea t o oec tubo isenuis encode eleva e Tan eta Gace ram rto 13 3 Gonnecuonm MeIDOGS uio ocu tiani ceptum NU Meu LIUM eM atl dk ole C UE 14 Chapters Microscope AdjUuSIME M22 eoi te ceo e e odere e Pete o evo se e mete oe sic te est eed 19 Chapter 6 Basic Operation Method ccccccccccssecccesceceececeneeeeeeeeceececeucessaeeeseeeeseusessasessaeeeseeeesenseeesaeeesees 22 1 Preparations Before Photographing ioi eter eie
46. access the internal DS L21MG folder of the DS L2 The latest image files that have been shot by the DS L2 are stored in the DS L21MG folder and these files can be downloaded to the PC NOTE Because the CCD image drive is read only no image files can be sent from the PC to the DS L2 The image files stored in the DS L21MG folder have size set in the file settings selected from File Settings of the DS L2 SETUP menu Alternatively if the settings are updated on the REC menu the updated size will be valid Example IMG1280 bmp IMG1600 bmp NOTE To capture files at maximum resolution you must set the correct parameters for size and the correct display mode The display mode also needs to be set to maximum resolution Setting the display mode to a lower resolution will result in image captures at the lower resolution The image files stored in the DS L2IMG folder are updated once every 5 seconds Be aware that if files are read out at intervals shorter than 5 seconds you will not always get the latest image To confirm the time of day at which image files have been updated follow the procedure described below 162 Chapter 6 Basic Operation Method USB 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function Confirming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed DS L2IMG
47. ae dead Sense dass actas sce ch dead uere E eei euh emet dae 217 3 DS Remote Controller DS RG anii oett oic tt ere E wick eie Pin E Speo que s tao e paa ends ve esp Gabe 218 AY DSC FAC apos uU uL MIU alc ME S 219 5 Overall SpeCiiCallOn TR 219 Vil CONTENTS Vill Chapter 1 Before Use 1 Types of DS Camera Head Before Use Types of DS Camera Head The following seven DS camera head models are available The features of the DS camera head vary depending on the model purchased Before use identify your DS camera head model Nikon oo 750 SIGHT ps rFi1 CE HIKON CORPORATIOM WADE IM JAPAN 110001 X i Nikon e 510001 BISITAL SIGHT DS 5Ml NIKON CORPORAT IC C y p MADE IN JAPA The model name of the DS camera head is found on the tripod mount or near the camera cap fitting position e DS camera head models and features 2 3 inch color 5 24 megapixel oed 2 3 inch color 5 24 megapixel gt 1 1 8 inch color 2 11 megapixel a For detailed information on the features of each DS camera head model refer to Chapter 9 Technical Specifications e Illustrations in this manual In this manual illustrations of LCD monitor screens such as menu displays are of the DS Fi1 Differences among models are described in Section 7 Operation Differences among DS Camera Head Models in Chapter 6 Chapter 1 Before Use 2 Components Components Fol
48. are subject to our license agreement Windows Users To see if your browser supports automatic installation please click here STEP 1 DOWNLOAD STEP 2 INSTALL STEP 3 VERIFY Windows Online Installation see below Instructions Verify Installation Note filesize 7 1MB Once you have installed Instructions Java software on your computer you must restart your browser You can verify that it Windows Offline Installation filesize 15 74 MB Solaris 32 bit filesize 20 32 MB Solaris 64 bit see below Note filesize 9 72 MB 20 32 MB 32 bit Solaris Instructions has been installed Instructions correctly by clicking on the Verify Installation Solaris x86 Instructions button above filesize 14 23 MB Solaris x64 see below Note bean filesize 4 92 MB Linux RPM self extracting file Instructions filesize 15 74 MB Linux self extracting file filesize 16 27 MB Instructions Linux x64 see below Note Instructions e A dialog box prompts you to open the file or save it to disk Select Save it to disk then click OK Opening jre 1_5 OQ 09 windows i5B6 p iftw exe The File jre 1_5 0 Q8 windaws ib86 p iftw exe is of type application x sdlc E and Netscape does nat know how to handle this File type This File is located ab http sdlc esd sun com ESDz28 J5CDL jdk 1 5 0 O9 bO3 What should Wetscape do with this File Open it with the default application ex
49. are labeled with the units nm um mm or cm If a unit other than these is being displayed set calibration values again 7 If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for Control License Card sold separately Fy Press the icon to hide all of the measurement results being displayed Fj Press the icon under this state to display them again i You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu i You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu Press the CLR icon to erase a measurement point existing on the image When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to erase it i Press the RCL icon to use a measurement point existing on the image for the next measurement When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to select it i Press the cu icon to save a measurement result in CSV format The destin
50. as some other device such as a camera Unable to print with a PictBridge compliant printer The printing icon for PictBridge zit does not show Unable to click the printing icon A PRINTER ERROR message is displayed during printing Chapter 8 Troubleshooting 6 USB Check points There may be a conflict between the drive letter assigned to the DS L2 and that of another USB device already connected to the PC Change the drive letter for the DS L2 referring to Section 5 3 2 Outline of the Disc Drive in Chapter 6 There may be a possibility that PictBridge is selected in USB Device in Power On Settings in the SETUP menu Select Mass S on how to use the USB device by referring to Section 1 4 5 Startup Condition Settings Power On Settings in Chapter 6 Confirm that PictBridge is selected in USB Device in Power On Settings in the SETUP menu Confirm that a PictBridge compliant printer is connected to the USB D connector on the DS L2 Confirm that the power for the PictBridge compliant printer is turned on In the power is ON but the printing icon is disabled close the menu and turn off the printer And then power on the printer and display the menu again Check the paper ink supplies and other conditions Restore the printer to the normal status by referring to the printer manuals and then display the menu to perform printing again 212 Chapter 8 Troubleshooting 7 Microscope Control f
51. be displayed in the changed mode after a while Using ROOM2 ROOM displays a list of image files saved to a CF card or USB memory The image files can be downloaded from here to the PC 4 6 3 2 1 Digital Sight DS L2 Bie ROOMI Relad EIS UTE Date Time ale Comment 2005 08 03 23 43 36 2005 08 03 23 43 16 2005 08 03 23 42 56 2005 08 03 23 42 36 2005 08 03 23 42 16 2005 08 03 23 41 54 2005 08 03 23 41 34 2005 08 03 23 41 14 2005 08 03 23 40 54 2005 08 03 23 40 34 2005 08 03 23 40 20 2005 08 03 23 39 56 2005 08 03 23 39 34 2005 08 03 23 39 16 1 Tabs Entrance Returns you to the Entrance screen ROOM1 Displays the ROOM 1 screen Reload Redisplays the ROOM screen ROOMS Displays the ROOMS screen This tab is not displayed if an unsupported combination of operating system and browser is used ROOMSA Displays the ROOM4 screen This tab is not displayed if an unsupported combination of operating system and browser is used 2 Location The DS L2 installation information etc that has been set by Telnet commands is displayed here Refer to Using ROOM1 described above for details on making these settings 3 Current folder name Shows the current folder name with the absolute path 4 Folders in the CF card or USB memory Shows folders in the CF card from the top level folder down to the second level folder Click any folder icon o
52. by pressing SAVE If the server cannot be logged in check settings on the PC side and those of the DS L2 145 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 4 6 3 Saving Image Files in the FTP Server Image files can be saved in the FTP server by using either the CAPTURE button or the REC menu 4 6 3 1 Using the CAPTURE Button to Save Image Files Saving image files in the FTP server The CAPTURE button on the DS L2 main unit or the DS Remote Controller DS RC may be used to save image files in the FTP server Before you can do this you must first select Save to Server for Capture Function in Additional Settings of the SETUP menu About the directory in which images are saved and the file name When an image file is saved in the FTP server using the CAPTURE button the file is stored in the folder specified on the DS L2 side at a location below the home directory of the server PC Also the file is assigned a file name according to settings on the DS L2 side The folder names and file names are set in File Settings of the SETUP menu For file names we recommend selecting Auto for File Name so that files will be named after the dates and times at which they are saved to the FTP server When images are sent via FTP the DS L2 does not check for duplicate file names Although file names are also suffixed with a serial number when Auto setting is not used if the power is turned off once the
53. call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 The value is selected automatically from through based on the hole number of the nosepiece you are currently using Refer to the instruction manual for i Control License Card sold separately 2 Press Calibration to bring up the calibration screen shown below 3 Press Optical to bring up the optical mode screen shown below Cee Calib W Calibe E E E Save 4 Click the numeric value input box to display the numeric keypad Use the keypad to enter the magnification of the objective 5 Click the Relay Lens numeric value input box to display the numeric keypad Use the keypad to enter the magnification of the relay lens 6 If you want to attach information to the calibration value you ve created click the Info input box and enter text for the information you want to attach This information will prove helpful because you can confirm Info when you call up the calibration value at a later time 7 Check the Set number to confirm that the calibration number is correct then click to save it is disabled if no value is entered 81 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu 8 Press to return to the previous menu Any calibration values not saved are deleted NOTE If the microscope connected to yo
54. can do this you must first select Save to Media for Capture Function in Additional Settings of the SETUP menu NOTE Refer to Section 2 1 1 Simple Photography Shortcut Menu for details on how to use the CAPTURE button to save images The following describes how to save images from the REC menu Configuration of the REC menu VIEW REC NEA ic Press to save an image to a CF card Press OF to save an image to a USB memory E D E F Press to save an image to an FTP server e save Press to save an image to a specified destination Dia Specify a folder name File Specify a file name OJ Cont inue Ey Tshot 1shot Only one image is saved Continue Interval photography photographed at given intervals Print e source Now 1200x1024 NOTE Refer to Section 4 6 Saving Images to an FTP Server PC from the DS L2 for details on how to save images to an FTP server from the REC menu 65 CAMERA Chapter 6 Basic Operation Method 2 Photographing Images In the REC menu you can specify the photographing source file format image size and compression rate when saving an image Choose the source either s5M for 5M cameras or s2M for 2M cameras to obtain high picture quality NOTE Refer to Section 7 Operation Differences among DS Camera Head Models in Chapter 6 for details about the source For the file format choose JPEG BMP or TIFF When a m
55. check box checked and the date is Chapter 6 Basic Operation Method 1 Preparations Before Photographing 2007 03 28 15 30 54 the image will be saved with a file name 28153054 When the save destination is a FTP server an image is saved with a file name comprising up to four characters of the File Name and eight characters representing the date and time twelve characters maximum Example If the File Name is DSL2 with the Auto check box checked and the date is 2007 03 28 15 30 54 the image will be saved with a file name DSL228153054 To prevent overlapping of file names using the Auto is recommended Format SEIUP Format an external medium CF card USB memory For details refer to Section 1 2 Notes on Using an External Recording Medium in Chapter 6 Setting an Human Interface Device HID HID Mapper The DS L2 can be controlled with a human interface device HID or a game pad through a USB interface Additionally quick operation is available by registering shortcuts such as Capture to each of the buttons provided to the HID Use a recommended product because some products may not function correctly The mouse pointer cannot be moved with the HID but the cursor can be moved Refer to 1 1 4 Using a Keyboard for moving the cursor To show the SETUP menu select the SETUP tag in the menu After selecting the HID Mapper on the SETUP menu the HID Game Pad Mapper screen appears as shown below Eac
56. files displayed at this time is O hours O minutes The actually acquired files are given the time of day when you issued the Get command 4 Execute the Get command for one of the files and the latest image that is in the DS L2 at that point will be downloaded to the PC A detailed command list is available from Nikon If you wish to have one please contact your nearest Nikon representative NOTE If frequent access is made to the DS L2 connected to a Windows PC via a USB cable image file acquisition may fail If this happens stop accessing the DS L2 via a USB cable for a while 137 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 45 Using Telnet Commands to Operate on the DS L2 You can use Telnet commands to operate the DS L2 from your PC NOTE Always be sure to consult the network administrator in your organization before executing Telnet commands Your organization s network may have been set up in a unique manner so invalid operations could cause a problem on your network Enter the IP address of the DS L2 from the MS DOS prompt command prompt of Windows to connect to the DS L2 Example Telnet 192 168 10 2 Commands Specify the commands listed in this manual A detailed command list is available from Nikon If you wish to have one please contact your nearest Nikon representative 138 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 4
57. head first attach the C mount cap Then use a soft cloth to wipe away dirt e Use a blower to blow away dirt inside the DS camera head mount When doing so be sure not to touch the glass filter on the front of the CCD If you do touch the glass filter or if dirt cannot be removed from it contact your nearest Nikon representative and have them clean it e Do not use organic solvents e g alcohol ether or paint thinner to clean the coated plastic or printed parts Doing so could result in discoloration or in separation of the printing 2 Storage e Turn off the power when the system is not in use e If the system will not be used for an extended period of time unplug the power cord e Do not store the system in an environment that is hot humid dirty or dusty e Attach the C mount cap when storing the system 203 Chapter 8 Troubleshooting 1 Power Supply Troubleshooting If you experience any problems check the troubleshooting list below If you are still not able to resolve the problem consult with your nearest Nikon representative 4 Power Supply Symptom The power does not come on The DS L2 is hot when touched Check points Be sure that the power cable is connected Check the power plug the AC outlet the AC adapter and the 12 VDC IN connector of the DS L2 Use the power cord supplied with the system Be sure to use a dedicated AC adapter Turn on the POWER switch The main unit
58. incorrect status information is displayed Change the registration if the objective lens or the filter has been changed 213 Chapter 8 Troubleshooting 8 Before Contacting Us 8 Before Contacting Us Before contacting Nikon or its sales partners please have the following information ready as this will soeed up the response to your inquiry Date Year Month Day Date of purchase Year Month Day Shop name Your name Your contact address Telephone DS camera control unit product name DS L2 Serial No Version information Number on lower right of Setup gt PowerOn screen DS camera head product name Serial No Peripheral devices connected USB mouse CF card microscope etc Manufacturer s name Model Manufacturer s name Model Manufacturer s name Model Your PC For PC related problems Manufacturer s name Model Operating system OS version and Service Pack SP number Type of CPU and its clock frequency Memory capacity Types of applications and their versions WEB browser NIS Elements etc Symptoms of the problem displayed messages and frequency with which the symptoms appear Please supply us with as much information as possible If there are problems in the images please supply us with the images if available Please copy this page and use it as required 214 Chapter 9 Technical Specifications 1 DS Camera Control Unit DS L2 Technical Specifi
59. information on these cameras refer to Chapter 9 Technical Specifications The following terms are used in this manual to refer to different cameras 5M cameras gt DS Fi1 DS 5M and DS 5Mc 2M cameras gt DS 2Mv DS 2MBW and DS 2MBWc Cooled cameras gt DS Qi1Mc DS 5M and DS 2MBWc Non cooled cameras gt DS Fi1 DS 5M DS 2Mv and DS 2MBW Monochrome cameras gt DS Qi1Mc DS 2MBW and DS 2MBWc Chapter 6 Basic Operation Method is explained taking a case of using DS Fi1 as an example Operation differences among models are described in Section 7 Operation Differences among DS Camera Head Models in Chapter 6 aD vx Chapter 6 Basic Operation Method SETU P 1 Preparations Before Photographing Preparations Before Photographing Using Various Input Devices 1 1 1 Using the CAPTURE Button CAPTURE button The CAPTURE button is used to save images When you press this button an image is saved in a destination selected in Capture Function of Additional Settings in the SETUP menu The destination can be selected from three options a recording medium an FTP server or a print output The DS L2 supports plural kinds of recording media When a recording medium is selected as a destination an image is saved in a recording medium selected on the REC menu 1 1 2 Using the DS Remote Controller DS RC FREEZE CAPTURE FREEZE button CAPTURE button FREEZE button This button allo
60. intervals Print o L C c L m source Now 1200 1024 NOTE Refer to Section 2 2 1 REC Menu Basic Function in Chapter 6 for details on how to set in the REC menu 147 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network Saving an image To save an image to the FTP server from the REC menu follow the procedure described below Press to set the FTP server as the destination Choose 1shot if you want to save only one sheet image Choose Continue to save multiple sheets of images at given intervals Change the destination or file name to or in which to save as necessary If you want to save a log enter a comment in LOG Press FTP and the image will be saved to the FTP server If you did not change the destination in step 2 the image will be saved using the folder name and file name that have been set in File Settings Refer to Section 1 4 3 Image File Settings File Settings in Chapter 6 for details on File Settings Also refer to the preceding sections for details about the recommended examples for file names etc Interval photography Interval photographing refers to photographing an image successively at specified time intervals To perform interval photographing follow the procedure described below Refer to Section 1 4 3 Image File Settings File Settings in Chapter 6 for details on file settings We recommended selecting Auto for the file name in File Setting
61. is a Nikon 80i 90i microscope with motorized or intelligent nosepiece the content drawn with the TOOL menu displayed on the screen items measured on T Bar is deleted when you switch the objectives Refer to the instruction manual for i Control License Card sold separately If the microscope connected to your system is a Nikon 80i 90i microscope with digital imaging head the content drawn with the TOOL menu displayed on the screen items measured on T Bar is deleted when you change the magnification of the optical zoom Refer to the instruction manual for i Control License Card sold separately If the microscope connected to your system is a Nikon LV Series microscope with a motorized nosepiece the content drawn with the TOOL menu displayed on the screen items measured on T Bar is deleted when you switch the objectives If the microscope connected to your system is a Nikon AZ100M microscope with an intelligent nosepiece the content drawn in the TOOL menu displayed on the screen items measured on T Bar is deleted when you switch the objectives Refer to the instruction manual for i Control License Card sold separately If the microscope connected to your system is a Nikon AZ100M microscope the content drawn in the TOOL menu displayed on the screen items measured on T Bar is deleted when you change the magnification of the optical zoom Refer to the instruction manual for i Control License Card sold separately ux
62. is a standard in which digital camera manufacturers and printer manufacturers guarantee interconnection between a digital camera and a printer so that an image on a digital camera can be output directly from a printer without using a PC as an interface For details refer to the official website of PictBridge http www cipa jp pictbridge zy Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu 3 Performing Measurements or Entering Information TOOL Menu EFT Outline of the TOOL Menu Display a menu and select the TOOL tag on it and the TOOL menu will be displayed In the TOOL menu the following functions are available to utilize the photographed image When you press T Bar the TOOL Bar to use meansurement functions is displayed In overlay you can perform measurements enter a comment or compare images laid on top of another You can place overlaid information on top of a photographed image before saving or printing the image Note however that the TOOL menu is subject to the following limitations A mouse is needed for almost all operations performed from the TOOL menu Although text can be entered from a keyboard you need a mouse to use this function The TOOL menu cannot be used in C Scan mode If you operate T Bar S Line Pen Mark or Text during electronic zoom the magnification is returned to an original one If the microscope connected to your system
63. l System Properties System Properties System Restore ll Automatic Updates Remote General Computer Mame Hardware Advanced Device Manager The Device Manager lists all the hardware devices installed on your computer Use the Device Manager to change the properties of any device Device Manager Drivers Driver Signing lets you make sure that installed drivers are compatible with Windows Windows Update lets you set up how Windows connects to Windows Update for drivers Hardware Profiles Hardware profiles provide a way for pou to set up and store different hardware configurations Hardware Profiles Right click the My Computer icon and select Properties from the shortcut menu 1 Click the My Computer icon 2 Click Properties Click the Hardware tab then click Device Manager D in the Hardware panel 1 Click here 2 Click here 180 Chapter Basic Operation Method USB 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function Device Manager window Click the Imaging Device icon to open it Device Manager File Action View Help o 2 E A8216 TESTER Click here Computer Disk drives 2 Display adapters A DVD CD ROM drives 4 Floppy disk controllers 4 Floppy disk drives c3 IDE ATA ATAPI controllers 338 Imaging devices gt Keyboards 3 Mice and other pointing devices Monitors BB Network ad
64. may not be able to select a format e Insert the connecting side with the connecting slot of a CF card into the card slot with the label side facing toward you Carefully note the direction and inclination of a CF card when inserting e Check to see that the CF card indicator LED is off before inserting or removing a CF card e Do not remove a CF card or turn off power to the DS L2 while the card is being accessed the LED indicator is on Attempting to do so may result in loss of recorded data or damage to the CF card e Be sure to use a USB memory whose operation is verified Not all USB memories are compatible with the DS L2 e fa USB memory is not recognized disconnect and reconnect it NOTE When a medium is connected to the DS L2 while the DS L2 is being operated the medium is heated by heat generated from the DS L2 A CF card or microdrive is heated more excessively since it is inserted in and connected to the DS L2 When the DS L2 is operated at 40 C maximum operating temperature the operating temperature of a CF card or microdrive becomes approximately 60 C Some media do not guarantee operation under the above operating temperature Do not use such media Medium formatting function The DS L2 can format a medium into a FAT type A FAT type is automatically set in accordance with the size of the medium FAT type approx under 2 MB FAT 12 approx 2 MB to 512 MB FAT 16 approx above 512 MB FAT 32 The DS L2
65. menu the items are saved in the image data if the corresponding functions are displayed When you deselect them they are not saved in the image data even if they are displayed on the screen Saving an image To save an image to a CF card from the REC menu follow the procedure described below 1 2 Choose 1shot if you want to save only one image sheet Choose Continue to save multiple image sheets at given intervals Select the drive and change the folder name or file name to or in which to save as necessary If you want to save a log enter a comment in LOG Press Save and the image will be saved to a CF card If you did not change the destination in step 2 the image will be saved using the folder name and file name that have been set in File Settings Refer to Section 1 4 3 Image File Settings File Settings in Chapter 6 for details on File Settings 66 2 2 2 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images NOTE When saving images on a CF card or USB memory device you can save the next image when the pinwheel cursor changes to an arrow cursor with a pinwheel REC Menu Convenient Function Interval photography Interval photography is a function to photograph an image successively at specified time intervals To perform interval photography and save the images in a USB memory follow the procedure described below We recommend selecting Auto for the file name in File Settings
66. mode is sixteen times more sensitive to light than other modes making it particularly useful for dark subjects Interlaced display modes may cause color shifts with moving subjects 184 Chapter Basic Operation Method CAM E RA VARI ETY 7 Operation Differences among DS Camera Head Models 5Mi An image area of 2560 1920 pixels is formatted for the monitor resolution CCD Mode 3 The frame rate is 5 9 fps This display mode is useful when viewing images in great detail We also recommend this mode when making long exposures Interlaced display modes may cause color shifts with moving subjects C Scan An image area of 1280 480 pixels corresponding to the center of the image CCD Mode 4 is formatted for the monitor resolution The top and bottom 1 4 are not displayed The frame rate is 23 3 fps This display mode is useful when good performance with moving subjects is required Progressive display modes eliminate color shifts and provide natural images even with moving subjects ROI An image area of 1280 960 pixels corresponding to the center of the image CCD Mode 4 is formatted for the monitor resolution The top bottom left and right 1 4 are not displayed The frame rate is 11 7 fps This display mode is useful when viewing images in great detail and when good performance with moving subjects is required Interlaced display modes may cause color shifts with moving subjects Select the REG gt gt tab on the CAM menu
67. move to at the gt position and press n playback button 2 Putthe cursor on a target folder name and the periphery of the folder name will be highlighted in yellow Click on it with the mouse Return to the upper folder in one of the following procedures 1 Locate lt DIR gt at the gt position and press n playback button Put the cursor on lt DIR gt and its periphery will be highlighted in yellow Click on it with the mouse Reproducing an image Images can only be reproduced in BMP JPEG and TIFF file formats Reproduce an image in one of the following procedures 1 Locate a file name you want to reproduce at the position and press n playback button 2 Putthe cursor on a target file name and the periphery of the file name will be highlighted in yellow Click on it with the mouse If there are too many files listed not all of the file names can be displayed on the menu If any files are not displayed on the menu use v Or Al to scroll the display up or down To use the keyboard to scroll move the cursor to V or A and press the Enter button Erasing images or folders Locate a file name or folder name you want to erase at the position and press erase button The file or folder will be erased Displaying a thumbnail Locate a folder name whose thumbnail you want to display at the position and press thumbnail display button A thumbnail of the folder will be displayed Place the
68. of the exposed image is complete Even if a signal is received during this time it has no effect Triggers can be received again after the falling edge of the read out transfer timing signal output L Triggered capture 1 ms or more e Trigger input l Exposure Dd Trigger input Read out transfer timing M Y possible Signal output lt 0 7 ms or less Approx 0 5 sec 0 6 sec or less V A Bulb shooting Exposure time setting Trigger input l Exposure Trigger input possible Read out transfer timing Signal output 0 7 ms 0 7ms Approx 0 5 sec 0 6 sec or less or less or less 190 Chapter Basic Operation Method CAM E RA VARI ETY 7 Operation Differences among DS Camera Head Models Size of saved images Select the size of saved images by specifying the CCD source and the size of saved images in the REC menu The CCD source setting represents the CCD mode during image acquisition when you freeze save or print images Select Source Full to switch the CCD mode automatically to 1 3Mp CCD Mode 1 during image acquisition for high resolution images Note that switching CCD modes causes a brief delay Select Source Now for image acquisition in a CCD mode matching the current CCD display mode This setting in which the CCD mode is not switched is recommended if you expect to freeze images often Source Now HHFF Specify the CCD source 12001024
69. on the image for the next measurement When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to select it i Press the cu icon to save a measurement result in CSV format The destination depends on the settings on the REC menu 5 Press the aX icon to select and delete a measurement result Press the a icon to update information being displayed 87 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu 3 4 8 Measuring the Distance between the Center Points of Two Circles You can measure the distance between the center points of two circles and save the measurement line as part of the overlay for the image 1 Press T Bar The tool bar shown below will be displayed Select the S icon NOTE Check if the S icon is selected 2 Press a color icon red blue green black white or yellow to set the color of the measurement line 3 Left click at three points for one of two circles and then at three points for the other to measure the distance between the center points of the two circles 4 A line is drawn between the center points of the two circles The measurement result will be displayed in the vicinity of the line To redraw a line use amp l o to cancel or redo your most recent action 5 Press the X icon or on the Tool menu to close the TOOL Bar NOTE Be sure to use these functions in the state where values
70. or black 5 Press the tab or the tab on the TOOL menu to bring up the tool shown below This tool allows you to select a display mode for the crosshairs that suits the subject whose position you want to adjust VIEW REC DE Radius Ini PM EUM Li Meo Geico Bp Miso Miso BL PUE pM pr M260 EUM Bil SUM ELIE E Uotted brass Line Ea Center Cross J talf Size Return to Center 6 Radius is an option that displays a concentric circle along with the crosshairs with respect to its center The numbers 20 to 360 in the Radius menu indicate the radius of the displayed concentric circle in pixel units Select the check box for any number to display a concentric circle whose radius corresponds to your selected number By selecting multiple numbers you can display concentric circles with different radiuses If you select the check box on the upper right the numbers are displayed in a unit used for calibration T Dotted Cross Line is an option that allows you to select the line type of crosshairs Select this check box and the crosshairs will be drawn with broken lines 8 Center Cross is an option that allows you to fill the center part of the crosshairs Select this check box to fill the center part of the crosshairs 91 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu 9 Press Return to Center to move the center position of the crosshairs ba
71. or features of camera operation vary with a combination of display mode and the source you use during photographing Refer to Section 7 Operation Differences among DS Camera Head Models in Chapter 6 Sample settings that suit different uses are described below Try these settings before determining your own settings e Fine adjusting the focus for a subject consisting of minute patterns 1 3Mp 5Mi Ex Semiconductor pattern low magnification photography of cells and tissue fluorescent photography landscape photography with a lens e Moving the subject while observing gt 1 3Mp Ex Semiconductor pattern stereoscopic microscope observation cell diagnosis general photography with a lens e Focusing on a dark subject gt 1 3Mi Ex Position or focus adjustment during fluorescent photography nighttime photography with a lens e Photographing a dark subject in the shortest time possible 1 3Mi Ex Fluorescent photography nighttime photography with a lens e Photographing a bright subject with priority placed on focusing C Scan 53 CAMERA Chapter 6 Basic Operation Method 2 Photographing Images 3 Choosing exposure mode Choose your desired exposure mode from PROG S AE MANU or F_AE Normally PROG should be selected Sample settings for different uses are described below Try these settings before determining your own settings Photographing a moving subject S AE combinatio
72. party 10 100BASE TX category 5 cable crossed To Ethernet connector oo ry 7 Connecting External I O Devices Be sure to turn off the power to the DS L2 and any equipment to be connected before making connections Set the external I O mode to Input by setting External Out to off in Additional Settings of the SETUP menu When an external device is connected to the EXT I O connector in this state the external device can be used to freeze or capture images Set the external I O mode to Output by setting External Out to on in Additional Settings of the SETUP menu When an external device is connected to the EXT I O connector a synchronized output signal can be obtained when an image is captured When the DS Qi1Mc is connected triggered capture from external devices is possible by supplying trigger signals to the EXT I O connector If you are preparing your own external device use a device that satisfies the specifications shown below Connector 63 5 mm diameter stereo pin jack Pin 3 GND Pin 2 Pin 1 16 Chapter 4 Connecting and Installing the Equipment 3 Connection Methods When using external devices to freeze or capture images Functions Pin 1 FREEZE Pin 2 CAPTURE You will not be able to capture desired images at an arbitrary timing Use of this input is not suitable for photographing fast moving objects using a sensor Pin 1 or 2 Activated wh
73. perform the settings again If you change the mode on the HID Mapper screen stop the registration and return to the original mode or press the and perform the settings again with the mode remained To prevent malfunction in the DS L2 refrain from using the HID that has unique functions such as the autmatic fire function macro function and button reassign function For details on the unique functions refer to the instruction manual for the HID 40 SETUP Chapter6 Basic Operation Method 1 Preparations Before Photographing Startup Condition Settings Power On Settings Display a menu and select the SETUP tag on it to enter the SETUP menu Select Power On Settings from the SETUP menu to display the Power On Settings screen shown below The following items can be set in the Power On Settings screen Registers Power On Settings Sets custom mode to be used at startup PowerOn Set NetWork Set File Set Additional HID Mapper Ret io Window Power n Settings PowerOn Settings No m 1 CAM Custom No m 0 turn off Short Cut Menu m small o Cross a Grid Cam OSD Bing FB Bio B etc Show tools OSD Position U Left Size Big USB Device EX Hass s a Vendor m r ICtB Language English me O43 Selects a kind of shortcut menus Sets functions of USB D connector Selects the language for the menu Shows crosshairs or grid lines when the DS L2 is started Selects a kind o
74. s paper size before being printed Real 10 print mode If the Mag Fix check box in Additional Settings is selected images are printed in real 10 mode In this mode images are enlarged or reduced when they are output in accordance with setting of the CMP value described later NOTE Printers supporting PictBridge produce different magnification depending on their model types For successful real 10 print mode be sure to adjust the CMP value after connecting your printer Adjusting the printing condition Use Additional Settings of the SETUP menu to adjust the printing condition CMP 90 110 Use it to adjust the scaling factor during real 10 mode For details on the adjustment refer to Section 7 5 Magnification for Observation and Printing in Chapter 6 frame Select printing with or without frame When this check box is selected an image is printed with frame 71 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images When selecting a PictBridge supporting printer only Selecting a Source described above is enabled Printing information at the bottom of the printed image is not possible In addition the settings for printing such as the paper size paper type and so forth are limited to the ones specified by the standard specifications of the printer you are using For details of the printer specification refer to the manuals coming with the printer About PictBridge standard PictBridge
75. same way as files are normally manipulated on a PC P Under the default condition the image files cannot be sent from a PC to a medium because the media drive is read out only ii When using the media drive with Windows 2000 to view the images captured while the images are written to the CF card with the FTP or REC menu choose a view other than thumbnail images To check the details click the image icon and start up a viewer Removing media When the DS L2 is connected to a PC follow the procedure described below to remove the media from the media drive 1 2 4 Check to see that the CF card indicator on the DS L2 is turned off Close the menus displayed on the DS L2 If any image files have been loaded from the media into the PC close all open image files on the PC Right click the media drive removable disk icon on the My Computer screen and select Eject My Computer File Edit View Favorites Tools d Back search 4 Folders Las LH x e Ez Address i My Computer My Computer Removable Disk T Removable Disk Capacity 60 3 MB Cl Used 22 6 MB Free 38 2 MB Ejects the removable disk From the drive o SA User C wee EP Psodat Seats earch a Control 344 Floppy A Local Disk C pLocal Disk Ds jLocal Disk E EILocal Disk F SS Compact Disc Removable Disk H Explore Sharing Call winzip Format
76. setting of the REC menu To switch the mode between the special mode and the normal mode use the following Telnet commands s BCDMDON The special mode function for the barcode reader is enabled s BCDMDOF The special mode function for the barcode reader is disabled The USB keyboard and the USB barcode reader cannot be used at the same time Be sure to use a USB barcode reader having keyboard interface 1 1 6 USB Game Pad The DS L2 can be controlled with a human interface device HID or a game pad through a USB interface Additionally quick operation is availabe by registering shortcuts such as Capture to each of the buttons provided to the HID Use a recommended product because some products may not function correctly For shortcuts registration method and other HID settings refer to 1 4 4 Setting an Human Interface Device HID HID Mapper in Chapter 6 26 Chapter 6 Basic Operation Method SETU P 1 Preparations Before Photographing 42 Notes on Using an External Recording Medium Images can be saved to or reproduced from a medium CF card USB memory Pay attention to the following when using a medium e Use a Type l or Type ll compliant CF card microdrive or a USB I F memory e Carefully read the users manual provided with your medium before using the card e To format a CF card on your computer use FAT12 FAT16 Sometimes these are expressed simply as FAT or FAT32 Depending on the OS used you
77. size diagonal size 163 mm e g 3 Magnification on the printed sheet 40 x 0 7 x 163 10 57 approx 430 times where the camera head is DS Qi1Mc the magnification of the objective is 40 times that of the relay lens is 0 7 times and the sheet size is L size diagonal size 163 mm Adjusting the magnification in the Real 10 mode Adjust the magnification in the following procedures 1 SETUP menu Output a printed sheet of a subject using the Test Print button in Additional Settings A printed sheet on which the scale in a pixel unit has been overlaid is outputted Nikon DS L2 Test Print In this example the actual dimension of this part is measured Scale display Printed sheet upper portion 2 Actually measure the dimension of the scale on the printed sheet e g The dimension from 0 to 500 pixels is measured 200 Chapter 6 Basic Operation Method CAM E RA VARI ETY 7 Operation Differences among DS Camera Head Models 3 Calculate the dimension of the scale on the subject surface using the following formula substituting the scale reading number of pixels Dimension of the scale on the subject surface Scale reading pixels x CCD pixel size Magnification of the objective x magnification of the relay lens lt Calculation examples gt e g 1 Dimension of the scale on the subject surface 500 pixels x 3 4um 40 x 0 7 approx 61um where the camera head is DS Fi1 DS 5M or DS 5
78. temperature may increase when used in a hot or confined space If the high temperature is accompanied by a burnt smell or other offensive smells turn off the switch unplug the power cord and ask for repair 204 Chapter 8 Troubleshooting 2 Image Output 2 o Image Output Symptom No image appears Only a crossline image appears on a white screen The image is out of focus The image is too dark or too bright Check points Connect the cables securely Connect the cables firmly in their specified locations Connect the DS camera head correctly Remove the C mount cap from the DS camera head Place the subject within the field of view of the microscope or the lens Direct the optical path of the microscope to the camera Set the exposure correctly See Section 2 Photographing Images in Chapter 6 Use a display compatible with the output format See Section 1 4 6 Adjustment for Using an External Monitor Connect the camera cable securely Set Sharpness on the CAM menu appropriately See Section 2 Photographing Images in Chapter 6 Turn off the electronic zoom function Focus the microscope or lens correctly Do not use the system in a vibration prone place Adjust the aperture diaphragm correctly See Chapter 5 Microscope Adjustment The aperture diaphragm should be adjusted to approximately 70 to 80 of the numerical aperture of the objective lens or 70 to 80 of
79. the FTP command operation by the Telnet command and operation with USB connection 186 Chapter 6 Basic Operation Method CAMERA VARIETY 7 Operation Differences among DS Camera Head Models DEZE 0550 unl 640 326 8 24 PROB Bla oP 00 Ae GAIN MU SFT s orr ws orr B amp H olset ei ee es ea es eo er CAES 5 3 Adv1 Menu Hi24 is selected E Ez oP E A GAIN ME SFT s orr ws orr B amp H olset ei ee es ea es c6 c7 OE e Adv1 Menu ROI32 is selected PROG CHP GAIN 100 Adv2 Menu Features of display modes 1 3Mp An image area of 1280 1024 pixels is formatted for the monitor resolution CCD Mode 1 The image is displayed at actual size 1280 1024 SXGA or reduced 1024 768 XGA Although the top and bottom portions of images are cut off when displayed at XGA resolution they are saved at full resolution 1280 1024 The frame rate is 19 fps Progressive display modes provide natural images even with moving subjects We recommend using this as the usual display mode 640 An image area of 640 480 pixels is formatted for the monitor resolution CCD Mode 2 The frame rate is 31 5 fps Because two pixels are added vertically and horizontally for each pixel created this mode is four times more sensitive to light than 1 3Mp mode making it useful for dark subjects 4320 An image area of 320 240 pixels is formatted for the monitor resolution CCD Mode 3
80. the Nikon 80i microscope equipped with DIH E or Nikon 90i microscope via USB the shutter for the episcopic illumination will automatically open or close corresponding to the DF DL scene mode operation This function is enabled only when observation method of the microscope on DS L2 is set for FL fluorescent microscopy For the setting for each observation method refer to the instruction manual for i Control License Card sold separately With this function the shutter opens by entering the DF FL scene mode in case the shutter has been closed Then when the exposure by the E exposure start button is completed the shutter closes When performing the focusing again with the focus button or performing the exposure again by the exposure start button the shutter opens again Further when selecting another scene mode or the custom mode to get out of the scene mode the shutter opens in case the shutter has been closed If the automatic shutter open close function is required in the Nikon 80i microscope equipped with DIH M input the following Telnet command from the PC w DEPION2 With this operation the shutter will always open close automatically while the microscope is used in the DF FL scene mode If you are photographing a dark subject that requires a long exposure time use the camera head in a cool place to allow its temperature to decrease This will help to reduce star shaped noise and increase the camera performance
81. the case of EVG staining a good result may be obtained when R of the color balance adjustment is set to 94 DIC PH Contrast is enhanced for differential interference and phase contrast photography purposes HE This mode is provided for photographing of HE stained specimen It is optimized for color reproduction specific to HE ELA This mode is provided for photographing of ELA Enzyme labeled antibody method It is optimized for color reproduction specific to DAB e Other scene modes Asbestos Red This mode is used to photograph the asbestos sepcimens This mode is optimized for the colors specific to chrysotile immersed in a liquid with refractive index of 1 550 crocidolite immersed in a liquid with refractive index of 1 680 and amosite immersed in a liquid with refractive index of 1 680 Asbestos Blue This mode is used to photograph the asbestos specimens This mode is optimized for the colors specific to crocidolite immersed in a liquid with refractive index of 1 700 Using DS 5Mc may cause poorer reproducibility than using DS Fi1 or DS 2Mv for some asbestos specimen We recommend using DS Fi1 or DS 2Mv for asbestos observation NOTE Compared to DS Fi1 or DS 2Mv reproduction quality for the asbestos specimens could be lowered on DS 5Mc We recommend DS Fi1 or DS 2Mv for asbestos specimens observation Custom mode Once the photographing conditions set on the CAM menu are sav
82. the custom mode number from through c7 4 Click the Info input box and enter a character string A string can be entered using up to 16 characters 5 When done press to save Although you cannot change the contents of the scene modes you can call up a scene once apply correction to it and then you can save it to c1 to c7 60 Chapter Basic Operation Method CAM E RA 2 Photographing Images 2 1 4 Special Photography on Advn Menu Fluorescent Photographing or Dark Subject Photographing The following settings are recommended for fluorescent photography or dark subject photography Settings on the CAM Advn menu Displayed only when the cooled camera is connected 1 Set the white balance under normal lighting conditions before CAH VIEW RED _ photographing Adjust R and B Gain as necessary TOOL SETUP Select small for the photometry area adjust for each rh subject Select b average photometry for the photometry method adjust for each subject When adjusting the focus first select OK DF FL scene mode Then right click on P focus button You will have high sensitive and high speed image display With the 1 3Mi display mode high gain display is obtained R10 TUI By that time the exposure mode has been automatically changed Brom HUE to MANU By adjusting the shutter speed and camera gain Dn TON 1 manually fine adjustment of the displayed image can be T performed
83. the image for the next measurement When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to select it Press the csu icon to save a measurement result in CSV format The destination depends on the settings on the REC menu Press the X icon to select and delete a measurement result Press the EA icon to update information being displayed 83 TOOL 3 4 4 Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu Measuring Perpendicular Distance You can measure the distance between a reference line and a point on an image The measurement line for this measurement can be saved as part of the overlay for the image 1 Press T Bar The tool bar shown below will be displayed Select the b icon NOTE Check if the ba icon is selected Press the red blue green black white or yellow color icon to determine the color of the measurement line Draw a reference line Click the mouse button at the start point of the reference line and click the mouse button at the end point to draw a line Click at the position of a point located at the desired distance from the reference line A perpendicular line measurement line will be drawn from where you click to the reference line The length of the measurement line will be displayed in the vicinity of it To redraw a line use llo Rn to cancel or redo your most recent action NOTE T
84. time NOTE If the DS L2 is left unattended for a long time without being connected to a dedicated AC adapter the timer may be delayed or stop Please remember that the timer will be delayed or stop after about four days have passed If you have left the dedicated AC adapter disconnected for several days check this setting before use Power Save Timer If you leave the DS L2 idle for a certain time it will automatically switch to power save mode You can set how long the DS L2 will wait before switching to power save mode unit minute Setting a value of 00 will disable the power save function Pointer speed Low Mid High Select the speed at which the mouse pointer moves across the screen when you move the mouse E Zoom Step Select the magnification rate of the electronic zoom at which rate the image is zoomed in or out Two or more choices can be selected Capture Function Save to Card Save to FTP Server Print Select one of these functions to save to a medium to save to the FTP server or to output to a printer you want to be assigned to the CAPTURE button on the DS L2 and the DS Remote Controller DS RC or to the CAPT button on the menu Two or more functions can be assigned to the CAPTURE button at the same time External Out This is an external interface output If you select ON a strobe synchronizing signal is output from the EXT I O connector when capturing an image For normal use we recommend selec
85. to your system is the Nikon 80i 90i microscope set the magnification of the digital imaging head optical zoom to x1 0 before calibrating Refer to the instruction manual for i Control License Card sold separately If the microscope connected to your system is a Nikon AZ100M microscope set the magnification of the optical zoom to x1 0 before calibration Refer to the instruction manual for i Control License Card sold separately 1 Select the number in which you want to save the calibration value from m4 through m7 The selection can be made in the calibration menu as well Drag the mouse pointer over one of numbers through m7 and the calibration information for that number will be displayed in the Info area for your confirmation 2 Press Calibration on the OTHER tab to bring up a calibration screen like the one shown below Calibration Mode PAME n A E Ro E CEAS 3 Draw a calibration line about the length of a specific subject on the image that you want to be the reference Click the mouse button at the starting point of the object to be measured To draw a line hold the mouse button while you drag it to the end point then release the mouse button To redraw a line use to erase the line you have just drawn 4 When you have finished drawing a line click the number input box to display a numeric keypad After entering any number press Ent 5 Select the unit of measure nm um mm cm 80
86. tone When you set the tone the contrast will change Sample settings are shown below Confirm tones on the monitor when you set a tone and select the most suitable one e To observe gradation in highlight reflections TONE 1 Recommended example IC chips part mounting circuit board solder component leads gears etc e To weaken or strength the standard contrast TONE2 weak TONE 3 normal TONE4 strong Recommended example General photography e To read the difference in luminous energy from the photographed image data gt LINR e To suppress unevenness in bright parts while photographing an image with clearly distinguishable dark and bright areas TONEG Recommended example Metal tissue or ceramics and other materials photographing documents with a lens etc e To enhance contrast for easy recognition TONE 7 Recommended example Metal tissue or ceramics and other materials photographing documents with a lens etc Features of tones There are seven kinds of tones Choose an easy to observe tone according to the subject TONE1 Wide dynamic range Use this tone when gradation from darks to brights is required as for subjects that have highlight reflections Recommended example IC chips part mounted circuit board gears etc TONE2 Weak contrast Use this tone when you want to weaken the contrast Recommended example Cells and tissue general subjects 56 Chapter 6 Basic Operation Method CAM
87. transmitted light to 1 16th of actual levels 7 Effects of Ambient Light When capturing a dark specimen with a fluorescent microscope for example ambient indoor light may enter the optical path Accordingly interior lighting should be darkened for photomicrography Place caps on the binocular eyepiece to prevent light from entering through the eyepiece 8 Effects of Vibration Because photomicrography involves extremely high image resolution the slightest vibration can affect image quality To insulate the microscope from vibration set it on a solid stable surface on a solid floor Using an anti vibration table compatible with the microscope will further reduce the effects of vibration While actual microphotography is in progress be careful to avoid touching the microscope or the desk or stand on which it is positioned 24 S EIU P Chapter 6 Basic Operation Method Basic Operation Method Preface to Instructions of Operation Method The DS L2 supports the seven camera heads listed below If you change camera heads the DS L2 control unit will automatically switch modes in response DS Fi1 5 megapixel CCD color non cooled DS 5M 5 megapixel CCD color non cooled DS 5Mc 5 megapixel CCD color cooled DS 2Mv 2 megapixel CCD color non cooled DS 2MBW 2 megapixel CCD monochrome non cooled DS 2MBWc 2 megapixel CCD monochrome cooled DS Qi1Mc 1 megapixel CCD monochrome cooled For detailed
88. 168 10 1 192 168 10 10 192 168 10 36 192 168 21 A wildcard can be used In the above example 1 to 254 are effective 4 Add an extension ip to the text file and save it in the NET directory or a directory hierarchically below it Example Autho ip 5 Insert the CF card or USB memory into the DS L2 6 Connect to the DS L2 from the PC by Telnet and run the command w IPA to register the IP addresses Refer to Section 4 5 Using Telnet Commands to Operate on the DS L2 in Chapter 6 When you wish to run the command w_ IPA insert either a CF card or a USB memory in the DS L2 The DS L2 searches NET directory of the CF card or the USB memory inserted and an IP address of a ip text file that the DS L2 has found from the directory is registered Saving logs of connected users NETLOG function A record can be kept of the IP addresses of the machines that accessed the DS L2 and of time information When accessed from a WEB browser access logs are kept only when the user IDs have been set in the Internal Server on the DS L2 side However these logs disappear when the DS L2 is powered off Run the Telnet command w_NLGON and the initial settings can be changed to display NETLOG To get the data contained in the logs access the DS L2 using an FTP command and get and read the file labeled NETLOG txt that is stored in the RAMIBOX directory or a directory hierarchically below it NETLOG txt is up to 64 Kbytes in si
89. 4 If the black level is weak after exposure this can be eliminated by adjusting the black level to the negative side using the SFT icon in the Adv2 menu To obtain the best image quality adjust the black level on the left side while checking the histogram CCD cooling temperature Regarding the allowable ambient humidity the DS Qi1Mc can be used under conditions of up to 80 RH Under these conditions set the CCD cooling temperature to 5 C less than the ambient temperature Set CCD Cooling temp to 5 C before use At an ambient humidity of 60 RH or less set the CCD cooling temperature to 10 C less than the ambient temperature Here set CCD Cooling temp to 10 C before use The DS L2 temperature will always return to 5 C after it is turned off For this reason after restarting it if you determine that the ambient humidity is 60 RH or less set the temperature to 10 C again 188 Chapter Basic Operation Method CAM E RA VARI ETY 7 Operation Differences among DS Camera Head Models Capture using an external trigger signal e Enable Ex Trigger Mode by selecting on to add ExTG as another exposure mode Choose ExTG in the exposure modes PROG S AE MANU F AE and ExTG to enable capture by means of a trigger signal supplied from external devices e Select for exposure timed to the falling edge of the trigger signal After image capture under t
90. 6 Saving Images to an FTP Server PC from the DS L2 In this instruction manual explanations are given assuming that the IP address of the DS L2 is 192 168 10 2 that of the server is 192 168 10 1 and that of the subnet mask is 255 255 0 0 while the gateway address is 192 168 128 1 In practice you should use the IP address assigned to you by the network administrator in your organization It is assumed that network settings on the PC side LAN connection have been completed before this function can be used Please consult the network administrator in your organization for details 4 6 1 Setting the FTP Server Functions of the PC For image files to be transferred from the DS L2 you need a PC which has FTP server functions Only basic settings are described here Refer to your OS s help function and other materials for more detailed information on settings 4 6 1 1 When Using Windows 2000 Professional XP Professional and Vista The following explains how to set FTP functions in Windows 2000 Professional XP Professional and Vista The Control Panel is displayed in a different way on the two operating systems e n Windows 2000 you select Start Settings Control Panel e n Windows XP and Windows Vista you select Start Control Panel Other than that almost all setting operations are common to both Setup procedure 1 Install Internet Information Service IIS Add FTP server functions 2 Add a use
91. C cable This cable enables display of nosepiece status No license required Chapter 3 Names of Parts and Their Functions 1 DS Camera Control Unit DS L2 Names of Parts and Their Functions 4 DS Camera Control Unit DS L2 Front of the DS L2 Built in LCD XGA 1024 x 768 pixels 8 4 inch display e q Power switch CAPTURE button Images are stored in a location selected with Additional Settings of the SETUP menu Chapter 3 Names of Parts and Their Functions 1 DS Camera Control Unit DS L2 Left side of the DS L2 LAN connector Connected to a LAN with a LAN cable to exchange data with a PC or a server USB D connector Connected to a PC with a USB cable to exchange data Connected to PictBridge compatible printer to directly print photographed images Camera connector Connected to the DS camera head with a camera cable Turn off the power when connecting disconnecting to avoid equipment failure DVI I connector Used to connect an external display Supports both analog RGB input displays and digital input displays 12 VDC IN connector Use the dedicated AC adapter Right side of the DS L2 CF card connector Insert a CF card into this connector to save or reproduce data to or from the card Make sure you re inserting the card in the correct direction USB H connector Connected to a USB device including USB mouse USB keyboard USB hub and USB memory Connected to the Nikon 80i 90i upright m
92. Control License Card sold separately E Select highlighted with a red frame the icon on the TOOL menu before moving the XY scales 3 5 5 Measuring Vertical and Horizontal Distances You can measure the horizontal distance on the x axis and vertical distance on the y axis between two crosshairs you display on the screen 1 Select a calibration value you want to use from through m7 2 Press C Msr Two crosshairs corresponding to the calibration value will be displayed 3 To move a crosshair click the center or one of the lines and drag to the desired position Move both crosshairs near the object to measure the portion of the object between the horizontal and vertical axes The x axis distance and y axis distance are displayed near the center of the crosshairs 4 Six colors are available red green yellow blue white and black 5 Toclear the display press C Msr once again Normally the axes for measuring vertical and horizontal distances are displayed on the screen but not printed on or saved with images If you select the C Msr check box on the OTHER tab you can draw the axes on saved or printed images NOTE E Displaying superimposed images while measuring vertical and horizontal distances will stop measurement and hide the crosshairs You can resume vertical and horizontal measurement after displaying the superimposed image Be sure to use these functions in the state where values are labeled
93. Create Shortcut Rename Properties Remove the media manually from the DS L2 155 15 xi USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 3 4 Using the CCD Image Drive Receiving image files Double click the CCD image drive removable disk icon on the My Computer screen to access the internal DS L21MG folder of the DS L2 The latest image files shot by the DS L2 are stored in the DS L21MG folder and these files can be downloaded to the PC NOTE Because the CCD image drive is read only no image files can be sent from the PC to the DS L2 The image files stored in the DS L21MG folder will have size set in the file settings selected from File Settings of the DS L2 SETUP menu Example IMG1280 bmp IMG1600 bmp NOTE To capture files at maximum resolution you must set the correct parameters for size and the correct display mode The display mode also needs to be set to maximum resolution Setting the display mode to a lower resolution will result in image captures at the lower resolution The image files stored in the DS L21MG folder are updated once every 5 seconds Be aware that if files are read out at intervals shorter than 5 seconds you will not always get the latest image To confirm the time of day at which image files have been updated follow the procedure described below 156 Chapter 6 Basic Operation Method USB
94. E 07 10 NF M380 6 1 2 M380ENO06 Nikon DS Camera Control Unit DS L2 DS Camera Head DS Fi1 DS 5M DS 2Mv DS 2MBW DS Cooled Camera Head DS Qi1Mc DS 5Mc DS 2MBWc lt Instructions gt Ver 3 2 Thank you for purchasing the Nikon products This instruction manual has been prepared for users of the DS Camera Control Unit DS L2 DS Camera Head DS Fi1 DS 5M DS 2Mv DS 2MBW and DS Cooled Camera Head DS Qi1Mc DS 5Mc DS 2MBWc To ensure correct use please read this manual carefully before using these products e No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon e The contents of this manual are subject to change without notice e Although every effort has been made to ensure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative e Some of the equipment described in this manual may not be included in the set you have purchased e f you intend to use any other equipment with this product read the manual for that equipment too e If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Warning and Caution Symbols Used in This Manual Although this product is designed to be completely safe during use incorrect usag
95. E RA 2 Photographing Images TONE3 Standard contrast Use this tone for ordinary photographing Recommended example Cells and tissue general subjects TONE4 Strong contrast Use this tone when you want to enhance the contrast The image will have clearly distinguishable darks and brights Recommended example Cells and tissue general subjects LINR Linear There is a linear output proportional to the input from the camera Use this tone when you want to examine the difference in luminous energy from photographed data Recommended example DIC observation PH observation etc TONE6 Provided for metal tissue Use this tone when you want to suppress unevenness in bright parts while photographing a high contrast image Recommended example Metal tissue or ceramics and other materials TONE7 Enhanced contrast Use this tone when you want to enhance the contrast during observation Recommended example Metal tissue or ceramics and other materials photographing documents with a lens etc uTN1 to 7 User tone Set arbitrary tone curves Draw with the mouse a tone curve on Tone Curve Control on the Adv2 menu by the drag operations The tone curve will be ultimately one into which the tone curve you have drawn is multiplied by a tone curve of the TONE number displayed on the tone curve control box If you wish to draw a tone curve from the scratch select LINR to do so For details on how to save a created tone curve refer to Sect
96. ETE What is an Overlay An overlay is something like a transparent sheet that is placed on top of a photographed image Text count markers pen drawings measurement lines or superimposed images can be written on the overlay The contents written to the overlay can be saved or printed along with the image Basic method of overlay operation All pen drawings text input etc for an image are written onto a single overlay sheet Follow the sequence shown below to create an overlay Choose the function l Draw or enter text You can choose to display or hide the overlay by using Overlay ON OFF Displaying or hiding the overlay Overlay ON displays the overlay on top of the screen Overlay OFF hides the overlay from the screen Even while Overlay OFF if you press any icon to draw on the overlay e g or Text the overlay will automatically reappear Erasing the overlay Press Clear All and will appear asking you to confirm whether or not to erase Press if you want to erase or if you do not You cannot partially erase an overlay All pen drawings count markers text measurement lines and superimposed objects will be erased Crosshairs scales grid lines and C Msr however will not disappear Saving or printing the overlay If you save or print an image while an overlay on it is being displayed in a state where the Paste to Image check box on the OTHER tab on the TOOL menu is selected the overlay imag
97. F FL scene mode press other scene mode or the custom mode 49 CAMERA NOTE When connecting DS L2 with the Nikon 80i microscope equipped with DIH E NOTE NOTE Chapter 6 Basic Operation Method 2 Photographing Images or Nikon 90i microscope via USB the shutter for the episcopic illumination will automatically open or close corresponding to the DF DL scene mode operation This function is enabled only when observation method of the microscope on DS L2 is set for FL fluorescent microscopy For the setting for each observation method refer to the instruction manual for microscope Control License Card sold separately With this function the shutter opens by entering the DF FL scene mode in case the shutter has been closed Then when the exposure by the exposure start button is completed the shutter closes When performing the focusing again with the gt focus button or performing the exposure again by the exposure start button the shutter opens again Further when selecting another scene mode or the custom mode to get out of the scene mode the shutter opens in case the shutter has been closed If the automatic shutter open close function is required in the Nikon 80i microscope equipped with DIH M input the following Telnet command from the PC w DEPION2 With this operation the shutter will always open close automatically while the microscope is used in the DF FL scene mode If you are phot
98. Forward Stc Synchronize z Wind Updat Address e about blank baka eA Show Related Links a a 39 B 4 53 z earch Favorites History Mail Size Print Edit Internet Options Left click here 4 Select Connections 5 Select LAN Settings Left click here General Security Content Connections Programs Advanced Use the Internet Connection Wizard to connect your computer to the Internet m Dial up settings G Wever dialle Tec Diel whenever a hewor Connection Is not present Always dial mi deraut connection Gurren detault hone Jv Beram system secuit check before dialing m Local Area Network LAN settings Left click here 106 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 6 Select Advanced If Advanced is disabled and you cannot select it left click on Use a Proxy Server first A check mark will be displayed allowing you to select Advanced Local Area Network LAND Settings Automatic configuration Automatic configuration may override manual settings To ensure the use of manual settings disable automatic configuration Automatically detect settings Use automatic configuration script Address m Proxy server Iv Use a proxy server Address Bar Advanced Bypass proxy server for local addresses Left click here nee T In the
99. LICENSE THE SOFTVVARE IDENTIFIED BELOW TO YOU ONLY UPON THE CONDITION THAT VOU ACCEPT ALL OF THE TERMS CONTAINED IM THIS BINARY CODE LICENSE AGREEMENT AND SUPPLEMENTAL LICENSE TERMS COLLECTIVELY AGREEMENT PLEASE READ THE AGREEMENT CAREFULLY B DOWNLOADING OR INSTALLING THIS SOFTWARE YOU ACCEPT THE TERMS OF THE AGREEMENT INDICATE ACCEPTANCE BY SELECTING THE ACCEPT BUTTON AT THE BOTTOM OF THE AGREEMENT IF YOU ARE NOT WILLING TO BE BOUND BY ALL THE TERMS Typical setup All recommended Features will be installed CO Custom setup Specify the features to install For advanced users Clear the check box for the program that will only install the Java plug in then click Next i J2SE Runtime Environment 5 0 Update 9 Google Google Toolbar 4nd Google Desktop Install Free programs with the Java Runtime Environment Search the Web with Google From any site Block annoying pop up ads a Autofil web Forms with a single click Privacv Policy Google Desktop Instantly Find your email Files media and web history Search directly From your desktop with Ehe deskbar Privacy Policy Bv installing these applications vou agree to the appropriate terms of use and privacy policy You can remove these applications easily ak any time 128 Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network e After a message is displayed indicating installation is complete click Finish ig J2SE Runtime Enviro
100. Lee red Linee rie ea eon excedens 23 1 1 Using Various Input Devices sees 23 1 1 1 Using the CAPTURE Button eeeesseeeseeeeeenneennnnennnem nnns 23 1 1 2 Using the DS Remote Controller DS RC sese 23 ti Ushoga Mouse a mesi et deud ades supe S desd tuens eddpsft cia ress cout ev Ve daba tuor 24 TI sing ING V DOAN du iach ous atem rodeo Mat nd aee osa nue 25 TLS USB Barcode Redd6el xdg oie vestis et Obs Ue stad ea ade Seca dot tu TE 26 LIO USB Game Fad eater wieght enteiden eatin 26 1 2 Notes on Using an External Recording Medium c cccccceeeeceeeeceeeeeneeeesaaees 27 1 3 Menu Configuration and Method of Displaying a Menu 28 Toad Mei ConfiglaliOEam scan ao dictada uiu d UR adu eee ee ad cd ated 28 1 3 2 Method of Displaying a Me Nu cccccccssscecceeececceeseecseeeecsseeesseueeesseeessaaeees 29 1 3 3 Using and Registering the Shortcut Me nu ccccccccseeeeeseeeeeeseeeeeeeeeeeseaaeees 30 NOS MEME 2 0 doc NUR t ER 32 tA Atal SOT Sasna eot te eden aie Als hoste eia i arnt chats 33 TAT Language Setting urit dator tei u te eie xee ta ieu ee epu te reta drei ees 33 1 4 2 Date and Power save Settings Additional Settings sss 34 1 4 3 Image File Settings File Settings cccccssececcsseeeceeseecseeseesseeeeessseesseaees 36 1 4 4 Setting a
101. Location el Click the X button to close the window Elements your Imaging Y Browse CD Select folder where setup will install files Click here Setup will install NIS Elements F 2 20 in the following folder To install to this folder click Next To install to a different folder click Browse and select another folder Dest ination Folder C Program Fi les NIS Elements F Browse Cancel For use of other applications exclusive to the DS L2 install them according to their instruction manuals 172 Chapter Basic Operation Method USB 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function 62 Installing Device Driver To connect a PC to the DS L2 the device driver must be installed in the PC For the DS L2 install two device drivers the Nikon USB DS Camera hereafter called camera driver and the Eclipse i Microscope hereafter called Eclipse i series driver The installation procedure is as follows N Caution Administrator login is required to install the DS L2 device driver 1 Close all the other applications To avoid failure in the setup program be sure to stop all system resident programs such as the screensaver and anti virus utility In this section the procedure for stopping the standard Windows screensaver is indicated For other programs see their instruction manuals Operation procedure Shortcut menu Right click on the
102. Mc the magnification of the objective is 40 times that of the relay lens is 0 7 times and the scale reading is 500 pixels e g 2 Dimension of the scale on the subject surface 500 pixels x 4 4um 40 x 0 55 approx 100um where the camera head is DS 2Mv DS 2MBW or DS 2MBWc the magnification of the objective is 40 times and the scale reading is 500 pixels e g 3 Dimensions of the scale on the subject surface 500 pixels x 6 45 um 40 x 0 7 approx 120um where the camera head is DS Qi1Mc the magnification of the objective is 40 times that of the relay lens is 0 7 times and the scale reading is 500 pixels 4 Adjust the CMP value in the Additional Settings of the SETUP menu so that the following formula is valid Magnification of the microscope Dimension of the scale on the printed sheet Dimension of the scale on the subject surface Magnification of the microscope Repeat test printing gt actual measurement adjustment of CMP value several times to approximate an accurate value 201 CAM E RA VARI ETY Chapter 6 Basic Operation Method 8 Connecting an LV Series Microscope 8 Connecting an LV Series Microscope You can use the specified USB 232C conversion adapter to connect a Nikon LV150A microscope to the DS L2 You can view information on the position of the LV150A microscope s motorized nosepiece and adjust the nosepiece position from the DS L2 menu NOTE Use only the sp
103. Mode 3 An image area of 1280 480 pixels corresponding to the center of the image is formatted for the monitor resolution The top and bottom 1 4 are not displayed The frame rate is 15 fps This display mode is useful when good performance with moving subjects is required Progressive display modes eliminate color shifts and provide natural images even with moving subjects C Scan CCD Mode 4 193 CAM E RA VARI ETY Chapter 6 Basic Operation Method 7 Operation Differences among DS Camera Head Models Size of saved images Select the size of saved images by doing CCD source setting and setting of size of saved images on the REC menu CCD source setting represents the CCD mode during image acquisition when you freeze save or print images When Source Full is selected the CCD mode is automatically switched to SMi CCD Mode 3 during image acquisition and images with high resolution can be obtained Note that switching CCD modes will result in a brief delay When Source Now is selected images are acquired in the current CCD display mode This setting in which the CCD mode is not switched is recommended when you expect to freeze images repeatedly CCD source settin Setting of size of saved images e5bxT8zl When the CCD source setting is Source Now and the CCD mode is 1 3Mp or 1 3Mi select 1280 960 or 640 480 for the setting of size of saved images When t
104. OOM4 to another screen click the tab located at the upper right corner of the screen Click the Reload tab to redisplay Do not use the Back button on your browser If the Back button is used the display may not be updated In that case select View Refresh on your browser e When moving to another screen the page may be distorted In that case select Refresh to display the page again e When you exit the browser the following exit message may appear In that case click on the OK button and close the window Microsoft Internet Explorer gt A It was finished e When the pop up block function such as the browser tool bar is active the subwindow may not be displayed In this case cancel the pop up block function referring to the given site Opening a WEB screen 1 Launch a WEB browser on your computer 2 Enter the IP address of the DS L2 in the URL address field Example http 192 168 10 2 The Entrance screen of the DS L2 will appear 3 Click ROOM1 ROOM2 ROOM3 or ROOM4 Digital Sight os 2 ROOMI Image Window with Control Pad ROOM2 CF Card amp USB Memory Access ROOMS ActiveX Site Windows IE Only ROOM4 iik ua Click ROOM1 ROOM2 Java Applet Windows IE Netscape Firefox MacOS Safari RO O M 3 O r RO O M 4 The display may appear faded for ROOMS or ROOMA if an unsupported combination of operating system and browser is used ROOM1 ROOM2 ROOMS and ROOM4 are explained i
105. PC or hub that will be used e USB 1 1 or USB 2 0 compliant cable when the PC or hub is USB 1 1 e USB 2 0 compliant cable when the PC or hub is USB 2 0 The DS L2 may not operate reliably if you use cables other than those specified 150 Chapter 6 Basic Operation Method USB 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function USB host drivers Use the USB host drivers made by Microsoft Corporation as the drivers included with your interface board may not operate properly e When using the USB 1 1 interface board The driver is included with the OS e When using the USB 2 0 interface board The driver is included with the OS providing it is Windows XP Service Pack 1 SP1 or newer If you are using Windows 2000 or Windows XP prior to SP1 download the USB 2 0 driver from the Microsoft Windows Update Web site Visit the Microsoft website for details on how to download drivers To install the USB 2 0 host driver that you have downloaded read the manual supplied with your interface board or consult the manufacturer OO 52 Connecting the DS L2 and a PC Follow the procedure described below to connect the DS L2 and a PC 1 Turn on the power to the PC main unit and start Windows 2000 Windows XP or Windows Vista 2 Start up the DS L2 On the Setup menu click the PowerOn Set button And then in the USB Device Mode check the Mass S checkbox 3 Restart the DS L2 4 Plug the A connector of the
106. S L2 from WEB browsers on several computers and displaying ROOMS the preview images displayed on those WEB browsers are the same The interval times for refreshing images are also the same The latest set values are applied to all of the WEB browsers Using ROOMA ROOMA displays the image currently being shot with the DS L2 at a specified interval and enables to capture the images display the stream screen and control the DS L2 on ROOMA screen Perform the settings of Java applets to use ROOM4 For the settings refer to 4 3 4 Java Applet Settings When executing ROOMA the warning may appear as shown below Warning Security The application s digital signature has been verified Do you want to run the application Name L24pplet Publisher Mikon Corporation From http 128 31 30 92 Cancel Mare Information g The digital signature has been validated by a trusted source 118 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network Select Always trust content from this publisher and click Run The program is certified with a digital signature This is the warning displayed in Internet Explorer but it may vary depending on your browser Some browsers may not always trust content from particular sources In this case a confirmation message may be displayed each time you access ROOM4 after starting the browser 2 3 4 8 5 1 9 The details of 1 to 9 are th
107. TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu Configuration of the TOOL menu VIEW REC ENES OE Clear All Erases overlay information Overlay ON OFF Displays or hides overlay Llear All Overlay ON dE oer en 1 Bar Displays TOOL Bar Displays XY distances Displays XY scales Displays a scale Displays crosshairs Displays grid lines ERI RSS a UJ o iO e IPs D 09 r 2 D Free form straight line function Pen function Mark Count mark function Text Text input function prf w2 ns ma ns non Info Superimposition Superimposition function ne Calls calibration settings Info Information display TOOL Bar Configuration Measurement functions Selection of measurement point Two points distance measurement Undo of measurement result E O Perpendicular measurement Redo of measurement result Recall of measurement point Deletion of measurement result X nt Angular measurement Erasing of measurement point CSV saving of measurement Circular measurement uU result Area measurement Display update Measurement of distance between center points of two Overlay ON OFF circles Pitch measurement Erasing of overlay information Ye E OD NM BI Close of TOOL Bar KA 74 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu
108. View Favorites Tools Help Back J x a A Search jT Favorites 2 a 3 Address http 128 31 30 36 r Internet Options General Security Privacy Content Connections Programs Advanced Select a W eb content zone to specify its security settings p Settings O Scripting Internet Local intranet Trusted sites Restricted Active scripting sites Disable Enable Local intranet Prompt This zone contains all Web sites that Allow paste operations via script are on your organization s intranet Disable Enable Prompt E Scripting of Java applets Custom Disable Custom settings Enable To change the settings click Custom Level O Prompt To use the recommended settings click Default Level p lleas Ae bh em bin nbion Security Settings Security level for this zone Reset custom settings Custom Level Default Level Reset to Medium low 122 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 2 Install the Java plug in If a Java plug in to run Java applets is not installed you will be requested to install a Java plug in the first time you access ROOMA Follow the instructions displayed to install it e Click Install Internet Explorer Security Warning Do you want to install this software Mame jinskall 1_4 windows i586 cab Publisher Sun Microsystems Inc L
109. W x 36 H mm AD 1260B 250 g F10453C 350 g F1650K 270 g AD 1260B GS CE and UL 5 O Overall Specifications Operating environment Temperature Humidity Altitude Pollution Installation category electric shock protection class Storage environment Temperature Humidity 0 to 40 C 60 RH maximum No dewdrops allowed DS Qi1Mc only Can also be used in 0 to 30 C and 80 RH 2000 m maximum Degree 2 Category 2 Class Indoor use only 20 to 60 C 90 RH maximum No dewdrops allowed The contents of this manual are subject to change without notice VCCI Voluntary Control Council for Interfer ence by Information Technology NOTE This equipment has been tested and found to comply with the specifications for a Class A information technology equipment pursuant to the standards of VCCI Voluntary Control Council for Interference by Information Technology This equipment is intended to be used in a residential area however it may cause radio disturbances if used near a radio or a television set Follow the instruction manual to operate the equipment properly 219 Conforming standards Chapter 9 Technical Specifications 5 Overall Specifications This product conforms to the UL60950 1 standard This product conforms to the EN60950 1 standard This product satisfies the FCC 15B Class A standard This product satisfies the EU EMC Directive This product satisfies the EU L
110. When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to erase it 89 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu Press the Rel icon to use a measurement point existing on the image for the next measurement When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to select it j Press the csu icon to save a measurement result in CSV format The destination depends on the settings on the REC menu i Press the icon to select and delete a measurement result i Press the c icon to update information being displayed 90 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu Adjusting the Subject Position and Comparing Sizes 3 5 1 Using Crosshairs to Adjust the Subject Position You can display crosshairs on the screen and use them to adjust the center or the horizontal or vertical position of the subject 1 Press Cross and crosshairs will be displayed 2 Point to the center of the crosshairs and click the mouse button Hold down the button while you move the mouse The position of the crosshairs moves as you move the mouse 3 When the crosshairs position is where you want it release the mouse button to set that position 4 The color of the crosshairs can be selected from six colors red green yellow blue white
111. above are saved to internal memory NOTE Saved grid lines settings will be kept until you execute again When you display grid lines for the first time after starting the DS L2 the grid lines will be displayed with the settings you last saved If you change the grid lines settings but do not save them you can display grid lines with settings you last saved at the next startup of the DS L2 93 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu 8 To hide the displayed grid lines press again Normally grid lines are displayed on the screen only and is not drawn on saved or printed images By leaving the Grid check box on the OTHER tab selected you can draw grid lines on saved or printed images NOTE b If you display a superimposed image while grid lines are on the grid lines will be hidden The grid lines can be redisplayed after you have finished displaying the superimposed image You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu n The minimum space between the grid lines is 20 pix When you set the narrower space no grid lines are displayed s Select highlighted with a red frame the icon on the TOOL menu before changing the color of the grid lines Grid lines display at power on The DS L2 can be set to display grid lines when it is powered on Setting from the SETUP menu Select ON for Grid in Powe
112. after called connected HIDMID and the ID at right shows the Maker ID of the HID registered to the checked Save Number hereafter called registered HIDMID NOTE When a Save Number not registered is checked the Maker ID and Product ID at right is indicated None Product ID now saved The Product ID registered in the HID appears The ID at left shows the Product ID of the HID in operation hereafter called connected HIDPID and the ID at right shows the Product ID of the HID registered to the checked Save Number hereafter called registered HIDPID NOTE When the connected HIDMID and the registered HIDMID are the same and the connected HIDPID and the registered HIDPID are the same the settings can be saved Likewise when the registered HIDMID and the registered HIDPID are None the settings can be saved However same HIDs can not be saved to two or more memory areas Data The last signal send from the HID to the DS L2 is displayed here Basic Keys Short Cuts function registration window Assign the following functions to each button of the HID Keyboard cursor key Up moves up the cursor Keyboard cursor key Down moves down the cursor Keyboard cursor key Left moves the cursor to the left Keyboard cursor key Right moves the cursor to the right Keyboard cursor key Enter executes the operation on which the cursor points Zhe te En Menu button displays and hides the Menu or displays a live ima
113. age When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to erase it Press the RCL icon to use a measurement point existing on the image for the next measurement When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to select it Press the cu icon to save a measurement result in CSV format The destination depends on the settings on the REC menu Press the a icon to select and delete a measurement result 2 Press the 3j icon to update information being displayed 86 3 4 7 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu Measuring the Area You can display a polygon on the image and measure its area and save lines and measurement results as part of the overlay for the image Press T Bar The tool bar shown below will be displayed Select the icon 1 NOTE Check if the icon is selected Press a color icon red blue green black white or yellow to set the color of the measurement lines Put point marks on the outer rim of an object whose area you want to measure After specifying the start point mark through the end point mark right click on an arbitrary position on the image The start point mark through the end point mark are connected and the area of the circled region is displayed To redraw a line use amp l Rn to cancel or redo your most rec
114. also be used for ceramic and plastic specimens Circuit Board This mode is suitable for photographing mounting defects on a part mounted circuit board Clipping of highlights in component leads and solder joints are suppressed allowing defects to be easily detected This mode is also suitable for high contrast subjects such as gears and other metal components FPD This mode is suitable for photographing the color filters for flat display devices such as liquid crystal display and plasma display NOTE On FPD scene mode better color reproduction could be obtained by setting color temperature of the display to 5000K which is close to the lamp temperature of microscope when you display images on an external screen If setting the color temperature on the display is difficult a better result could also be obtained by setting B of the color balance adjustment to 80 However there would be a difference in histogram curve even after white balance adjustment 46 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images e Biological specimen scene modes DF FL Dark field photography fluorescent photography Use this mode to photograph a dark subject The camera operability for position or focus adjustment is improved by increasing the camera s sensitivity High resolution images may be obtained from the photographed data BF Bright field photography This mode is provided for general stained specimens In
115. and the network settings of the PC e g the IP address Also check that the PC is connected to a LAN by using a browser or the ping command e Set exception items in the browser s proxy settings Deselect the Read Only check box in Network Settings of the SETUP menu Checking the set values Settings on the DS L2 gt e Make network settings in Network Settings of the DS L2 SETUP menu Enable Network IP Address Always be sure to make gateway settings if data communication between different network groups is to be performed e g sending or receiving data to and from a distant place of business Settings on the FTP server PC Check settings on the FTP server Check FTP server functions Check access rights Testing a connection login to the set server After making network settings on the DS L2 perform a ping test and the login test 209 Symptom Images are overwritten when saved to an FTP server The FTP send button cannot be pressed The Capture button does not respond even though it has had Save to Server assigned Even though the FTP send button is pressed the timer icon keeps running for about one minute and no images are sent An FTP error results when sent to the FTP server The Capture button is deactivated immediately after it is pressed Images cannot be saved to the FTP server even though the DS L2 is connected to it and no errors are displayed The FTP server
116. apter 6 Basic Operation Method 2 Photographing Images 2 2 3 Reproducing Images from a Medium VIEW Menu Display a menu and select the VIEW tag on it to enter the VIEW menu In the VIEW menu you can reproduce images on the screen from an external medium CF card USB memory You can also delete image folders and files from the external medium or display a thumbnail Drive letters Locate the file name or folder name you want to reproduce at the w position Selecting a drive letter When a medium is connected to the DS L2 one of the drive letters is assigned to the medium One of the four drive letter icons C D E and F on the menu which corresponds to the connected medium is changed from gray to white In the case of a CF card C is assigned to it without exception When a USB memory is connected either of D E and F is assigned to it Select a drive letter in the following manner 1 Put the cursor on a target drive letter and the periphery of the drive letter will be highlighted in yellow 2 Click on it with the mouse 3 The periphery of the selected drive letter is highlighted in red When a drive letter is selected the folder file name in the medium is displayed on the menu 68 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images Moving to another folder Move to a lower folder in one of the following procedures 1 Locate a folder name you want to
117. apters Y Ports COM amp LPT BR Processors Sound video and game controllers System devices Universal Serial Bus controllers Z Device Manager Two imaging devices must be listed Nikon USB DS File Action View Help Camera Nikon USB Eclipse i Microscope E amp 22 Sie 4 48216 TESTER Computer See Disk drives 2 Display adapters A DVD CD ROM drives 4 Floppy disk controllers Floppy disk drives Sy IDE ATA ATAPI controllers 38 Imaging devices 338 Nikon USB DS Camera 338 Nikon USB Eclipse i Microscope Se Keyboards gt Mice and other pointing devices Monitors HB Network adapters F Ports COM amp LPT 888 Processors C h e ck h ere Sound video and game controllers System devices Universal Serial Bus controllers 181 USB Chapter 6 Basic Operation Method 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function 63 Operation The operation method of the DS U2 differs depending on the application used together 1 For the NIS Elements freeware Function of each icon on the Main Window is described below lt NIS Elements Freeware Main Window gt NIS Elements F Live Fast Cog File Edit Camera k Q Point s View Help A gt 5 d 3 amp H a Q Qa Zoom Annotate AE Area Focus Open Save s rini Fit Original Zoomin ZoomOut 63 5 solution ocus
118. arches Jg Public 2 Right click any image file icon of interest The updated date and time will be displayed below ej c NEN DD gt Com puter NIKONIMG G DS L2IMG z Wy Organize ML Ws v jm Preview WW SlideShow m Print Mf E mail Burn ESOS Name Date taken Tags Size Rating i IMG1280 E Documents E Pictures Ay Music jj Recently Changed BB Searches Wd Public Folders IMG1280 Rating Camera maker Nikon Metering mode Average TIFF Image Dimensions 1280 x 960 Camera model DS 5M L2 Date created 11 17 2005 1 14 PM Size 3 51 MB Exposure time 1 sec Date modified 11 17 2005 1 14 PM 169 USB USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 5 5 Removing the DS L2 from the PC Because the DS L2 is connected via a USB interface it can be removed from the PC even while the PC remains powered on Under the default condition where formatting a medium writing files and deleting files in the media inserted to the DS L2 are disabled follow the removal procedure described below 1 Eject the media from the media drive before proceeding 2 Unplug the USB cable or turn off the DS L2 If you have used Telnet commands and modified the default condition so that formatting a medium writing files and deleting files in the media inserted to the DS L2 are enabled you need to prevent data from being corrupted whil
119. at is being shot by the camera head of the DS L2 from a WEB browser Even from a distance you can check what image the DS L2 is currently shooting When you access the DS L2 from a WEB browser a WEB screen is displayed on which you can operate the DS L2 There are four WEB screens ROOM1 ROOM2 ROOM3 and ROOM4 available depending on your needs e ROOM1 Displays the image currently being shot at a specified interval Also allows you to control the DS L2 e ROOM2 Allows you to load saved image files from a CF card or USB memory into the PC e ROON3 Displays the image currently being shot at a specified interval Also allows you to control the DS L2 Suitable for live images because enables to set the time interval shorter than ROOM1 ROOMS is operative when the DS L2 is used with the following OS and browser OS Browser Windows 2000 SP 4 XP SP 2 Internet Explorer 6 02 SP 2 7 0 Vista e ROOM4 Displays the image currently being shot at a specified interval Also allows you to control the DS L2 Suitable for viewing images in real time because the interval can be set shorter than in ROOM1 ROOM4 is available with the following combinations of operating systems and browsers Browser Windows 2000 SP 4 XP SP 2 Internet Explorer 6 02 SP 2 7 0 Vista Netscape 7 1 Firefox 1 5 2 0 Mac OS X 10 3 or higher Safari 1 0 Firefox 1 5 2 0 ROOMS can display images at a faster rate than ROOM4 if you specify an interval
120. ated on the image processing software in the PC Create it with the width of 1280 pix the height of 960 pix and the depth of 4 bit Up to two data files can be registered Create each file in a BMP bitmap format as SIPDT1 and SIPDT2 The Superimposition images disappear when another Tool function is displayed 99 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 4 Using the DS L2 via a Network Typical Method of Use You can connect the DS L2 and a PC in a network and operate the DS L2 from the PC or exchange image files between the DS L2 and the PC There are three methods for operating the DS L2 from the PC e Use a WEB browser e Use an FIP command e Use a Telnet command You also can access an FTP server PC from the DS L2 via a network Possible operations in each method are described below The symbols A and p gt in the diagrams below indicate the flow of images while the symbol gt indicates the flow of control Using a WEB browser to operate the DS L2 from a PC e Get images from the DS L2 e Control the DS L2 in a simple way oHm Pe I A WEB browser may be used from a LAN based PC to observe images In this case the DS L2 s HTTP server function is used NOTE As a rule although it depends on how frequently images are updated the DS L2 can only be accessed through up to four simultaneously displayed WEB browsers at the same time 100 Chapter 6 Basic O
121. ath for the home folder on the Profile tab The specified path should be below the home directory however Example If the home directory is C DSFTP the user home folder local path of the DS L2 is C DSFTP DS1 and the FTP folder on the DS L2 side is FTPIBOX then the FTP transfer directory is C DSFTP DS1 FTPIBOX 3 Set local paths for the second and the third users DS L2s as C DSFTP DS2 and C DSFTP DS3 That way you can specify a separate directory for each user siai N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 4 6 1 2 When Using Mac OS X Setup procedure 1 Setting up the FTP server Add FTP server functions 2 Adding a user entering the user ID and password Set the DS L2 as a log in user This setting must be made after logging in with the user ID of the system administrator Settings Setting the FTP server 1 Select Apple Logo System Environment Settings 2 For Internet and network items in the System Environment Settings window click Shared 3 Select the check box Start FTP Service for Mac OS 10 1 on the File and Web tab or the check box FTP Service for Mac OS 10 2 on the Service tab Adding a user 1 In the System Environment Settings window click User for Mac OS 10 1 or Account for Mac OS 10 2 2 In the User window for Mac OS 10 1 or Account window for Mac OS 10 2 click
122. ation depends on the settings on the REC menu i Press the X icon to select and delete a measurement result Press the m icon to update information being displayed 88 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu 3 4 9 Measuring the Pitch You can measure the distance perpendicular distance between a reference line and an arbitrary point on an image and the distance pitch between two perpendicular lines The measurement lines for this measurement can be saved as part of the overlay for the image 1 Press T Bar The tool bar shown below will be displayed Select the S4 icon NOTE Check if the EY icon is selected 2 Press the red blue green black white or yellow color icon to determine the color of the measurement line 3 Draw a reference line Click the mouse button at the start point of the reference line and click the mouse button at the end point to draw a line 4 Click at the position of a point located at the desired distance from the reference line A perpendicular line measurement line will be drawn from where you click to the reference line The length of the measurement line will be displayed in the vicinity of it Also click at the position of another point located at the desired distance from the first perpendicular line A second perpendicular line will be drawn from where you click to the reference line and a pitch measurement line
123. ave the data that has been set Or press Default to restore data to default values Media Dir FTP Dir Specify the folder name in which you want files to be stored For Media Dir specify a folder name used in a medium CF card or USB memory For FTP Dir you can use up to eight characters to specify a folder name used in an FTP server If you select the Auto check box a folder is created automatically according to the date on which you save an image The folder thus created is assigned a name that consists of the first two characters of your specified folder name and the six characters representing the date Example If the specified folder name in Media Dir is IMGBOX with the Auto check box selected and the date is 2007 03 28 then a folder named IM070328 will be created File Name Specify up to four leading characters for the name of the file in which you want to save an image A photographed image is saved under a file name comprising the specified file name and an automatically assigned serial number The serial number recycles back to No 1 when you turn off the power When the Auto checkbox is checked the file name creation method changes depending on the save destination a medium and a FTP sever When the save destination is a medium an image is saved with a file name comprising a total of eight characters representing the date and time 36 1 4 4 Exmaple If the File Name is DSL2 with the Auto
124. awn 4 To withdraw from the operation mode press again or press another icon You cannot erase the drawn content without pressing the 3 icon to erase only the drawn content or erasing the entire overlay m NOTE You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu 76 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu 3 3 2 Straight Line Drawing The straight line function allows you to draw a free form straight line on the image NOTE You need a mouse to use this function 1 Press S Line 2 Press the red blue green black white or yellow color icon to determine the color of the line 3 Click at the starting point hold down the mouse button while you drag and unclick at the ending point and the straight line will be drawn 4 To withdraw from the operation mode press S Line again or press another icon You cannot erase the drawn content without pressing the 2 icon to erase only the drawn content or erasing the entire overlay mE NOTE You can draw a normal line and an arrow line respectively by selecting the Normal and Arrow check boxes of the S Line item on the OTHER tab on the Tool menu You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu 377s TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering I
125. b on the Tool menu a You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu i When only the drawn content is erased by pressing the 2 icon the counter value is not cleared 78 3 3 4 Chapter Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu Entering Textual Comments lot No 1123 The text function allows you to enter a textual comment in the image NOTE Although text can be entered from a keyboard you need a mouse to perform other operations Press Text Move the cursor to a position where text is to be input and left click on it The following window appears Press the red blue green black white or yellow color icon to select the text color Click the input window with the mouse to display a keyboard on the screen You will see the words Input Mode displayed on the upper left part of the menu Click the characters on the keyboard that you want to enter When you have finished press to confirm your entry If you missed entering any character use to erase all characters or to erase one character To enter characters from your keyboard move the cursor to the text input window and press the Enter key Use the SHIFT Backspace keys on the keyboard to erase all characters or the Backspace key to erase one character Type your comment on the keyboard and hit the Enter key to confirm the content of your
126. be displayed in the vicinity of it Use or to cancel or redo your most recent action Press the JX icon or on the Tool menu to close the TOOL Bar NOTE i Be sure to use these functions in the state where values are labeled with the units nm um mm or cm If a unit other than these is being displayed set calibration values again a If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for i Control License Card sold separately Fi i Press the icon to hide all of the measurement results being displayed Fy Press the icon under this state to display them again j You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu i You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu j Press the CLR icon to erase a measurement point existing on the image When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to erase it z Press the ROL icon to use a measurement point existing on
127. be used to mount the DS camera head on a tripod 2 Connecting an External Display Be sure to turn off the power for the DS L2 and the display before making any connections When using a digital display use a digital DVI cable a cable with DVI D 24 pin connectors at both ends Connect this cable to the DIV I connector on the DS L2 and the digital input terminal of an external display When using an analog RGB display use an analog DVI cable a cable with the DVI l 29 pin at one end and the D sub 15 pin at the other end Connect this cable to the DIV I connector on the DS L2 and the digital input terminal of an external display Secure the connections using a screwdriver to tighten the screws on the cable connectors 14 Chapter 4 Connecting and Installing the Equipment 3 Connection Methods 3 Connecting USB Peripheral Equipment Be sure to turn off the power for the DS L2 before making any connections A USB mouse USB keyboard USB memory Nikon 80i 90i microscope LV150A LV100DA LV ECON industrial microscope or AZ100M Universal Zoom Microscope can be connected When only one USB device is connected connect the device to either one of the two USB H connectors on the DS L2 When two devices are connected simultaneously connect each of them to the USB H connector When three or more devices are connected simultaneously use a USB hub Only one USB hub can be connected to the DS L2 4 Connecting to a PC or a Pi
128. between the two perpendicular lines will be drawn The length of the measurement line will be displayed in the vicinity of it To redraw a line use Sl Rn to cancel or redo your most recent action NOTE To redraw a reference line select the 52 icon once again 5 Press the 2X icon or on the Tool menu to close the TOOL Bar NOTE i Be sure to use these functions in the state where values are labeled with the units nm um mm or cm If a unit other than these is being displayed set calibration values again i If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for Control License Card sold separately rj i Press the icon to hide all of the measurement results being displayed Fj Press the icon under this state to display them again i You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu Press the CLR icon to erase a measurement point existing on the image
129. c 06 c7 NOTE If shading is excessive correct calculation data Into may not be obtained aaa 3 Registering the tone settings Create tone settings by the tone creation function on Adv2 After the tone settings are created the t1 to t7 buttons become available Select values you wish to register and press to complete the registration Refer to Section 2 1 3 Fine Adjustment before Photographing Advn Menu 5 Choosing tone in Chapter 6 for details on how to create the tone 4 Registering the camera settings Select values c1 to c7 you wish to register and press to complete the registration You can write a comment by clicking on the Info section Select the registered items on the CAM Adv1 menu Regarding the REG2 menu refer to Section 7 Operation Differences among DS Camera Head Models in Chapter 6 64 Chapter Basic Operation Method CAM E RA 2 Photographing Images 22 Using a CF Card USB Memory 2 2 1 REC Menu Basic Function Select the REC tag at the top of the CAM menu screen The REC menu will be displayed In the REC menu you can specify a saving destination medium a folder name and a file name You can also choose interval photography which lets you shoot an image successively at given intervals In addition to the REC menu you can use the panel switch on the main unit or the CAPTURE button on the DS Remote Controller DS RC to save images to a medium Before you
130. cales close to the subject before measuring its dimensions 1 Select the calibration value you want to use from m1 through m7 2 Press C Scale to display scales corresponding to the selected calibration value 3 Point to part of the X scale and click the mouse button Hold down the button while you move the mouse The position of the X scale moves as you move the mouse 4 When the X scale position is where you want it release the mouse button to set the position of the X scale 5 The position of the Y scale can be moved in the same way 6 To hide the displayed scales press C Scale again Normally XY scales are displayed on the screen only and are not drawn on the saved or printed image If you select the C Scale check box on the OTHER tab you can draw the XY scales on saved or printed images NOTE P It may not be possible to display XY scales depending on the selected calibration value If XY scales cannot be displayed a message C Scale NG is displayed in the Info area In that case change the calibration unit that has been set for example from 1 mm to 1000 um Be sure to use these functions in the state where values are labeled with the units nm um mm or cm If a unit other than these is being displayed set calibration values again B If you perform an electronic zoom while using this function the screen display will reflect the zoom magnification P If you change the optical
131. cations 4 DS Camera Control Unit DS L2 Display LCD display External output format Camera control Exposure control Photometry Electronic shutter Exposure compensation White balance Color correction Image correction Operation Scene mode Custom setting Tool functions Other functions 8 4 inch TFT liquid crystal display 1024 x 768 XGA Digital DVI output DVI standards 1 0 or analog RGB output 0 7 Vp p 750 SXGA XGA selectable SXGA 1280 x 1024 60Hz non interlaced XGA 1024 x 768 Q 60Hz non interlaced Sync signal V H separate sync TTL level VESA standards Program AE shutter speed priority AE focus AE manual exposure Average photometry peak hold photometry photometry area position size selectable When DS Fi1 DS 5M DS 2Mv or DS2MBW is connected 1 1000 sec to 60 sec When DS Qi1Mc DS 5Mc or DS 2MBWc is connected 1 1000 sec to 600 sec Compensation range 42 0 step 1 3 Set method color balance adjustment Gamma tone black level saturation hue adjustment special effects monochrome inversion Shading correction noise reduction edge enhancement horizontal vertical inversion of image Selectable from a total of five biological and a total of four industrial modes Seven modes Measurement functions distance angle circumference diameter area count marking function text input function pen function XY measurement superimpose function scaling functio
132. cense purchased separately NOTE For information on purchasing a microscope control license please contact the nearest Nikon office or distributor 44 Chapter Basic Operation Method CAM E RA 2 Photographing Images o2 o Photographing Images EET Setting Camera Operations and Adjusting Images 2 1 1 Simple Photography Shortcut Menu Right click on the lower right of the screen or press the button on the task bar in a normal state to display the Shortcut menu Two types of Shortcut menu Small and Large are featured as standard for the DS L2 The Shortcut menu allows you to photograph an image easily Data reproduction button PictBridge button Medium save button WB button Exposure compensation button NOTE The small shortcut menu shown above is displayed at the time of the initial startup Two kinds of shortcut menus Small and Large are provided as a standard and besides shortcut bars user1 user2 user3 to which the user can set arbitrary functions are provided For details refer to Section 1 3 3 Using and Registering the Shortcut Menu in Chapter 6 Photographing procedure To photograph an image from the Shortcut menu follow the procedure described below 1 Select the scene mode or the custom mode Select either of the modes according to the subject and observation method 2 Adjust the white balance Prepare an evenly white subject and adjust the white balance with WB
133. ck to the center of the screen 10 To hide the displayed crosshairs press again Normally crosshairs are displayed on the screen only and is not drawn on saved or printed images By leaving the Cross check box on the OTHER tab selected you can draw crosshairs on saved or printed images NOTE i If you display a superimposed image while crosshairs are on the crosshairs will be hidden The crosshairs can be redisplayed after you have finished displaying the superimposed image You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu E Select highlighted with a red frame the icon on the TOOL menu before changing the position and color of the crosshairs Crosshairs display at power on The DS L2 can be set to display crosshairs when it is powered on Setting from the SETUP menu Select ON for Cross in Power On Settings on the SETUP menu and press SAVE NOTE Even if you have set the DS L2 to display crosshairs at power on the crosshairs will be hidden if you choose to hide it or use a superimpose function If this happens redisplay crosshairs by using the menu 202 TOOL Chapter6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu 3 5 2 Using Grid Lines to Adjust the Subject Position You can display grid lines on the screen and use them to adjust the center or the horizontal or vertical position of the subject
134. comment The Input Mode message disappears The characters are displayed on the specified position To withdraw from the operation mode press again or press another icon You cannot erase the drawn content without pressing the 2 icon to erase only the drawn content or erasing the entire overlay m NOTE You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu You may only enter alphabetical characters numbers and symbols 79 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu Measuring on the Screen Once you calibrate the reference length you will have a simple means of measuring distances You can save seven calibration values for each objective or measurement condition and reuse saved values by calling them up the next time you perform a measurement 3 4 1 Performing Calibration and Saving Values Before measuring the length of anything you must first calibrate the reference length Be aware that you cannot perform measurement if you skip the calibration process There are two ways to calibrate You can use a subject of known length or you can use the magnification of the objective and relay lenses mis Icon when using a subject of known length Y Calib Icon when using the magnification of the objective and relay lenses lt When using a subject of known length gt NOTE Ifthe microscope connected
135. ctBridge Compatible Printer with a USB Cable When connecting to a PC connect a USB 2 0 cable AB type to the USB connector on the PC and the USB D connector on the DS L2 When connecting to a PictBridge compatible printer connect the printer and the USB D connector on the DS L2 with a USB cable AB type When using a PictBridge printer set the USB Device Mode to Printer in the Power On setting of the Setup Menu 5 Connecting the DS Remote Controller DS RC Be sure to turn off the power for the DS L2 before making any connections Connect the EXT I O connector on the DS L2 and the TO EXT IN connector of the remote unit with the remote cable that comes with the DS L2 When using the remote unit set the External Out to off in Additional Settings of the SETUP menu 6 Connecting to a Network Be sure to turn off the power for the DS L2 and the destination device before making any connections In addition be sure to consult your network administrator Make the connections as shown below We recommend using cables and hubs designed for 10 100BASE TX use 15 Chapter 4 Connecting and Installing the Equipment 3 Connection Methods Connecting to a LAN DS L2 Network 4 Third party 10 100BASE TX A category 5 or 5e cable straight a To Ethernet Third party 10 100BASE TX connector category 5 or 5e cable straight Connecting to a PC without going through a LAN DS L2 Third
136. d 1 Preparations Before Photographing Shortcut menu Set a kind of the shortcut menus You can select Small or Large and the three user settings user1 user2 user3 For how to use and register the shortcut menus refer to Section 1 3 3 Using and Registering the Shortcut Menu in Chapter 6 Show tools Set whether or not crosshairs or grid lines are shown when the DS L2 is started Refer to Section 3 5 1 Using Crosshairs to Adjust the Subject Position in Chapter 6 and Section 3 5 2 Using Grid Lines to Adjust the Subject Position in Chapter 6 Cam OSD Ind Bio etc Select a kind of scene mode buttons displayed on the menu Industrial or Biological The industrial mode is useful for photographing specimens of semiconductor wafer liquid crystal panel circuit board and metal structure The biological mode is useful for bright field stained specimens and fluorescent specimens The other mode etc is useful for photographing asbestos specimens USB Device Select how to use the USB D connector Mass S The USB D connector is connected to the PC to use mass storage function In the mass storage function you can read write from on an external medium inserted in the DS L2 or take photographed images in the PC Refer to Section 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function in Chapter 6 Vender Connect to a PC and execute the vender unique function The vender unique functi
137. d adjust the focus while watching the image on the monitor If you have difficulty in adjusting the focus because the subject is dark or the exposure time is long the DF FL scene mode is recommended For operation method of the DF FL scene mode refer to Section 2 1 2 Simple Photography in the DF FL Scene Mode in Chapter 6 4 Compensating the exposure Use EE exposure compensation button to adjust the brightness of a screen d When clicking the exposure compensation button the button design changes to Click the button to adjust the brightness 5 Saving the image Use feed to save the image You can also use 41 to print the image The image is saved in accordance with the REC menu with regard to the folder name file name file format and compression rate Refer to Section 2 2 1 REC Menu Basic Function in Chapter 6 or Section 4 6 3 Saving Image Files in the FTP Server in Chapter 6 for details on how to use the REC menu Use p gt data reproduction button to reproduce saved image data 48 Chapter Basic Operation Method CAM E RA 2 Photographing Images 2 1 2 Simple Photography in the DF FL Scene Mode To simply photograph a dark subject or fluorecent specimen use the DF FL scene mode and the large shortcut menu Exposure compensation button Capt button Pictbridge button Exposure start button Fucus button Media save button Operation in the DF FL scene mode is different
138. d is compatible with the system Mac OS is not supported Supported OSs Windows 2000 Service Pack 3 SP3 or higher XP SP 2 Vista Check that the USB Device Mode operation method of the Power On Settings on the SETUP menu is set properly For mass storage function select Mass S and for vendor unique function select Vender Check that the USB host driver made by Microsoft Corporation is installed The driver included with the USB board may not work properly when performing mass storage function Check that a camera driver and Eclipse i series driver are installed when performing vendor unique function Images are updated once every five seconds Be aware that if files are loaded into a PC at intervals shorter than five seconds the captured image may not always be the latest one A warning in the task tray system tray such as HIGHT SPEED USB Device Plugged into non HI SPEED USB hub is displayed when the USB 1 1 compliant port is connected This is not an error DS L2 operates as a USB 1 1 device 211 Symptom The DS L2 behaves as follows after being connected Although a CF card is inserted into position a Please insert media message is displayed if you click the icon for the media drive Asimilar message is displayed if you click the icon for the CCD image drive Other USB devices already connected are disabled When connected to a PC DS L2 is recognized not as a storage unit but
139. d the image files are saved in that directory 140 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network Example If the home directory is C DSFTP and the FTP folder specified on the DS L2 side is FTPIBOX then the FTP transfer directory is C DSFTP FTPIBOX NOTE The contents described above can also be set form Control Panel Administrative Tools Internet Information Services Other settings When enabling logon as an anonymous user In order to discriminate the user by which image files are written to the FTP server we in the above settings adopted a method to log in with a user ID and a password However it is possible to log in as an anonymous user 1 When setting External Server in Network Settings of the DS L2 select anonymous for the User ID and none for the Password 2 Enable the FTP server PC for anonymous access As in home directory settings select the Allow Anonymous Logon check box on the Security Account tab in the Preset FTP Sites properties window Be aware that if you select the Allow Only Anonymous Logon check box no one but an anonymous user can log on When specifying a directory in which image files are saved separately for each of multiple DS L2s connected 1 Select Computer Management Local System Tools Local Users and Groups Users 2 Double click the user you want to set and specify a local p
140. din forhandler eller lokale myn digheter Se Symbol for separat upphamtning i euopeiska lander Den har symbolen anger att produkten maste hamtas separat F ljande g ller bara anv ndare i europeiska lander e Den h r produkten r avsedd f r separat upph mtning vid ett l mpligt uppsamlingsst lle Produkten f r inte kastas i hush llsavfall e F r mer information kontakta terf rs ljaren eller de lokala myndig heter som ansvarar f r avfallshantering E Gi Erillisen kerayksen merkki Euroopan maissa T m merkki osoittaa ett tuote ker t n erikseen Seuraavat maininnat koskevat vain eurooppalaisia k ytt ji e Tama tuote ker t n erikseen asianmukaisista ker yspisteist l h vit tuotetta talousj tteiden mukana e Lis tietoja saat j lleenmyyj lt tai paikallisilta j tehuoltoviranomaisilta E Rul CUMBON COPTMPOBKH mycopa MCNONBb3yIOLM CA B EBpPONE CKNX crpaHax laHHbl CUMBON O3HaYaeT YTO TOT NpOAYKT AONXKEH yTADM3MpOBATbCR OTAENbHO OT Apyrux lipaBeneHHaR Hwxe UHCbOpMaLVA Kacaerca TONbKO norib3oBare en n3 CTpaH EBportbi e laHHbllA NPOAYKT DOMKEH yTWIMSMpOBATbCR OTAENbHO OT Apyrux B COOTBETCTBYIOLUUX npueMHeix rnyHKTax He Bbl6pacbiBalre J1aHHbli nponykTr BMecre C ObITOBbIM MyCOpOM ononuurernibHyro uHepopMauuro Bbi Moxere nonyuurb y npogasya MNN y MecTHbIX BNACTE orBeuaroulux 3a yTWIWMSaLMto Mycopa Izd Gr Z p o o yia TNV amp EXopiorr anoKopi arroppiupi ro
141. does not function correctly due to other troubles Chapter 8 Troubleshooting 5 Network Check points When images are sent via FTP the DS L2 does not check for duplicate file names Select Auto for File Name in File Settings of the SETUP menu and time information will be added to each file name helping to avoid overwriting files Although file names are also suffixed with serial numbers even when Auto setting is not used if the power is turned off once the serial numbers recycle and start from 1 again Therefore it is possible that one of the existing files will be overwritten For FTP folder too selection of Auto will help to avoid overwriting files because directories are separated by date If the DS L2 is not connected to the AC adapter for more than a week its internal timer will stop If the DS L2 is not used for an extended period of time reset the time of day The backup function recovers when the AC adapter is connected to supply the power Check the network cable and hub connections e Select the Enable FTP Client check box in Network Settings of the SETUP menu This is because the IP address of the External Server that has been set in Network Settings of the SETUP menu cannot be found Check the IP address of the server one more time If images are sent across a gateway specify the Gateway address Use the ping test function to confirm This is because the DS L2 has not logged in to the FTP server
142. ds because if the button is pressed too quickly the Test Screen may not respond NOTE Ifa light displays at a wrong position perform the settings again according to 9 the procedure 5 and 6 Press the Ret to Window to end the settings NOTE The registered HID when connected to the DS L2 at start up can be used without resetting Cautions on Setting the HID Use the recommended product because some HIDs Game Pad may not function correctly Do not use two HIDs simultaneously If an HID is not connected no item can be registered on the HID Mapper screen Do not touch the HID button when pressing the Clear All When pressing the Clear All the HID data registered to the memory area is completely cleared So specify all settings again An HID cannot be registered to two Save Numbers Also two HIDs if they are the same model can be registered to only one Save Number Two or more functions cannot be registered to one button of the HID To rewrite a new HID data to the memory area in that other HID data is already saved press the Clear All to clear the registered data Even if using the registered HID on the HID Mapper screen the registered function does not operate but a light displays on the Test Screen When using the HID whose analog digital modes can be switched only one mode can be registered To register another mode change the mode and press the on the HID Mapper screen and then
143. e indicator lights up in green Chapter 3 Names of Parts and Their Functions 1 DS Camera Control Unit DS L2 N CAUTION e f this product fails or loses its control the Power indicator blinks in red Check the error message on the screen And then take countermeasures following instructions in the message If no error message appears turn off the power of the DS L2 Unplug the power cord from the wall outlet And then contact your nearest Nikon representative e When you extract or replace the stand arm care must be taken to prevent your fingers from being caught between the stand arm and the main unit Rear of the DS L2 Vent hole upper rear part For internal cooling Vent hole lower rear part For internal cooling Chapter 3 Names of Parts and Their Functions 2 DS Camera Head 2 DS Camera Head Tripod mount This mount can be used to mount the DS camera head on a tripod Bottom Rear C mount cap CAMERA OUT connector Protects the C mount from dust C mount When taking microphotographs mount the C mount adapter here before connecting to the microscope When taking other photographs attach the C mount lens here 3 DS Remote Controller DS RC Optional To EXT IN connector Insert the supplied remote cable here to connect to the EXT I O connector of DS L2 FREEZE CAPTURE FREEZE button Freezes releases the current i
144. e Check that the User ID and Password on the DS L2 side and the user account on the server side match Check settings on the server side to see if the FTP server function is active e Check settings on the server side to see if the server is enabled to write e f images are sent across a gateway specify the Gateway address Use the login test function to confirm whether the DS L2 is logged in Check settings on the server side to see if the server is enabled to write Refer to 4 6 Saving Images to an FTP Server PC from the DS L2 in Chapter 6 210 USB Symptom Data cannot be transferred normally Cannot be connected normally When images are successively downloaded it is the same image that appears on PC A warning is displayed on the PC Chapter 8 Troubleshooting 6 USB Check points Make sure the USB 1 1 interface board and the USB 1 1 hub used in the system are compliant with the USB 1 1 standards and that the USB 2 0 interface board and the USB 2 0 hub used in the system are compliant with the USB 2 0 standards Check that no hub is cascated to the hub if used between the interface board ant the DS L2 Nikon recommends using one hub or none Make sure the USB cables used in the system are USB compliant When the DS L2 is connected via USB 1 1 use USB 1 1 or USB 2 0 compliant cables When the DS L2 is connected via USB 2 0 use USB 2 0 compliant cables Check that the OS version use
145. e is written over the photographed image In this case the photographed image and the overlay are integrated into a single image when saved so you cannot erase the overlay from the saved image If the overlay is hidden while you save or print only the photographed image is saved or printed e Some text may be dropped when saving an overlay over an image if you save at the minimum file size Furthermore if the image is saved in JPEG format we recommend selecting a low compression rate such as Fine or Normal e For details refer to Section 1 4 3 Image File Settings File Settings in Chapter 6 75 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu 330 Entering a Line or a Comment on the Image You can enter a line or a comment on the overlay by using the pen drawing count marking text or free form straight line function An overlay can be placed on top of a live image If you do not want the image and a comment to be mismatched use the freeze button to freeze the image before entering a comment s Free form Drawing The pen function allows you to draw a free form curve on the image NOTE You need a mouse to use this function 1 Press Pen 2 Press the red blue green black white or yellow color icon to determine the color of the pen 3 Click at the starting point and hold down the mouse button while you drag and the locus of the pointer will be dr
146. e level below Y100 causes the output saturation level to drop Color density CHRM To increase the color density in the image being displayed adjust CHRM to the positive side To reduce the density of color adjust CHRM to the negative side The density of color can be adjusted in the range from 50 to 50 Hue HUE Use this facility when you want to rotate hues in minute color adjustments Watch the image while you make adjustments Also be careful not to forget to reverse previous settings Effects COLOR B amp W NEGA BlueB Sepia You can choose color effects on an image from the following five choices e COLOR This is the normal color image output e B amp W This is a monochrome image Use this effect for monochromatic subjects such as DIC or PH and metal tissue e Nega With the brightness reversed this is used as a Special effect e BlueBack Blacks in Nega are turned blue This is used as a special effect 58 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images e Sepia With colors developed in sepia this is used as a special effect Shading correction SD OFF SD10 to SD 50 SD US1 to 7 Correct unevenness in shades on the screen To correct for a reduction in luminous energy from the middle part toward the periphery of the screen use 10 to 50 correction data Watch the screen while you select the correction data When you are to correct nonsymmetrical unevenness etc acquire and sav
147. e not indicated after performing the procedures again the HID is not applicable Likewise when the Data value does not change restart the DS L2 and perform the procedure 1 and 2 If the Data value does not change after performing the procedures again the HID is not applicable 4 Select the memory number with the Save Number Then function buttons and are enabled NOTE If the operations do not become enabled see the Cautions on Setting the HID in this section 5 Click a function button on the Basic Keys Short Cuts and then press an HID button for the function When the Set OK appears assignation is ended NOTE If the message is NG perform the procedure 5 again If it is NG again see the Cautions on Setting the HID in this section Press the Clean All and restart the DS L2 and then do the settings 39 SETUP Chapter 6 Basic Operation Method 1 Preparations Before Photographing Repeat the procedure 5 as necessary To cancel the assignation during settings repress the function button Then the Cancel is displayed and the assignaion is canceled Additionally press the on the function registration window to clear all the keys assigned After registering all the required functions press the Save To check that the settings are correct press the HID button to which a function is registered and check that the corresponding location on the Test Screen lights up Press the HID button for one or more secon
148. e or failure to follow the safety instructions provided may cause personal injury or property damage To ensure correct usage read this manual carefully before using the product Do not discard this manual and keep it handy for easy reference Safety instructions in this manual are marked with the following symbols to highlight their importance For your safety always follow the instructions marked with these symbols Symbol Meaning N WARNING Disregareing instructions marked with this symbol may lead to serious injury or death ZN CAUTION Disregarding instructions marked with this symbol may lead to injury or property damage Microsoft Windows Internet Explorer Active X and Direct X are registered trademarks of Microsoft Corporation in the United States and other countries Macintosh MacOS and Safari are registered trademarks of Apple Computer inc in the United States and other countries Netscape is registered trademark of Netscape Communications Corporation Fire Fox is registered trademark of Mozilla Corporation Opera is registered trademark of Opera software ASA Java and other trademarks including Java are trademarks or registered trademarks of Sun Microsystems Inc in the U S and other countries Pentium is a registered trademark or a trademark of Intel Corporation in the United States and other countries The PictBridge logo mark is a trademark Products and brand names are trademarks or registered trademarks of their re
149. e page you re viewing has information that requires a browser plug in Sound complicated It s not The Netscape Plug in Finder has already done most of the work for you Below is a list of plug ins that will enable you to view the information on the page Click the Download button for the plug in you would like to use Business amp Utilities Image Viewers Presentations Computing amp Internet Tech News Hardware Software Developer Help How To To learn more about Netscape plug ins click here bill 5 Plug in Developers Please try Netscape search to find your Create a Plug in plug in How to Embed Plug ins Search for Plugins supporting All Languages S E e If the following screen appears click the Java link amp Plug in Search Netscape lal x a File Edi View Go Bookmarks Tools Window Help 2 amp Plug in Search AD Netscape in or amp Signin network ABOUT NETSCAPE NETWORK Mail AIM Radio Maps Home gt Computing amp Internet gt Download gt Browser Plugins gt Plug in Search Departments What s N eee Find the Plug in You Need 3D amp Animation Th ds DC Asin tion that DeC e page you re viewing has information that requires Business amp Utilities Image Viewers Presentations 5 Computing amp Internet Tech News Hardware Software Developer Help How To 5 Plug in Developers Create a Plug in How to Embed Plug ins
150. e removing the DS L2 during a writing operation To ensure this follow the procedure described below 1 Eject the media from the media drive before proceeding 2 Shut down the PC 3 Unplug the USB cable or turn off the DS L2 NOTE Since the DS L2 is a composite device the Safe to Remove Hardware icon does not appear when the DS L2 is connected to the PC as per Microsoft FAQ Article ID 927826 Always shut down the PC before removing the device for the time being until an update program to solve this problem is made available from Microsoft 170 Chapter 6 Basic Operation Method USB 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function When connecting a PC to the DS L2 using USB and executing the vender unique function using a special application on a PC the DS L2 can be controlled and the live display or capture of the photographed image can be performed BETEM Installing Application Software To operate the DS L2 application software must be installed in the PC connected to the DS L2 via a USB interface We recommend the NIS Elements Freeware for application software exclusive to the DS L2 The NIS Elements Freeware can be downloaded according to the website of the Nikon Instruments Company URL www nikon instruments jp jpn service download DLList aspx CID 3 After downloading install the so
151. e same as those of ROOM 1 Refer to the usage for ROOM1 In the Interval Timer marked with 3 MAX can be selected The MAX is not provided to ROOM1 When selecting MAX the preview images are refreshed in real time Images can be displayed faster in ROOMS than in other rooms when Internet Explorer 6 02 SP 2 or 7 0 is used as a browser and the operating system is Windows 2000 SP 4 XP SP 2 or Vista Under these conditions we recommend using ROOMS If ROOM3 cannot be displayed on your computer try using ROOM4 When one DS L2 is accessed by multiple computers at once and ROOM3 or ROOMA is displayed the same preview image will be displayed for all users Additionally the same refresh interval time will be used This is because the most recent settings are applied to all browsers 119 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 4 3 3 ActiveX Settings The ActiveX controls must be enabled to use ROOM3 The settings can be performed only when the Internet Explorer 6 02 SP 2 or 7 0 is used on the Windows 2000 SP 4 or the XP SP 2 operating system The setting procedure is as follows 1 In Tools select Internet Options 3i Welcome Microsoft Internet Explorer File Edit View Favorites BEES Help 1 Mail and News gt gt n Q Back QJ ix Pop up Blocker pP es ue 3 Address amp http 128 31 30 3 PUES a Synchronize Windows Update
152. e same way through the WEB screen through FTP or Telnet commands or over a onnection 191 CAM ERA VARI ETY Chapter 6 Basic Operation Method 7 Operation Differences among DS Camera Head Models Saving in RAW format With the DS Qi1Mc you can also save images in RAW format in addition to the preceding file types shown in the table In RAW format 12 bit monochrome RAW data is saved as TIFF images This format is recommended if you will edit the images on a computer Additionally the image file size can be reduced to two thirds that of a regular TIFF file by saving in RAW format Refer to the following table for image file sizes File Type RAW TIFF 1280 1024 2 6MB 1280 720 1 8MB 640 480 600KB 320 240 150KB However note the following when saving in RAW format e When the CCD source is Source Now in the REC menu images can only be saved in the size of the current CCD display mode When the CCD source is Source Full images can only be saved in 1280 1024 size e n RAW format images are saved just as they are captured on the camera Thus images being displayed on two screens or images that have been edited using the Tool menu cannot be saved Only live images can be saved e Images saved in RAW format on the DS L2 cannot be opened by some browsers e Images saved in RAW format on the DS L2 are darker than images in other formats 192 CAMERA VARIETY Chapter 6 Basic Operation Method 7 Operation Di
153. e size of 2560 1920 If you select an image size of 2560 1920 it will be automatically reduced to 1280 960 for DS Fi1 DS 5m DS 5Mc cameras 70 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images 230 Outputting Images Directly to a Printer 2 3 1 Printing an Image When operating from a shortcut menu or the REC menu use the print icon to print A print icon is displayed When operating from the DS Remote Controller DS RC you must choose Print for Capture Function in Additional Settings of the SETUP menu before you can print NOTE If a printer is not connected to the system the icon is displayed translucently In this case the icon cannot be selected If any printer error such as paper out occurs during printing the operation of the DS L2 may not be completed correctly In this case cancel the print job on the printer and perform restoration operation such as paper feed and then perform printing again with the print icon on the menu 2 3 2 Various Print Related Settings The following various settings can be chosen Choosing print mode The direct print function allows you to print in either normal or real 10 mode Choose print mode in Additional Settings of the SETUP menu Normal print mode Unless the Mag Fix check box in Additional Settings is selected images are printed in Normal print mode by default In this mode an image is enlarged or reduced as will be suitable for the printer
154. e the contents of the shading correction in SD US1 to 7 in advance and use SD US1 to 7 for the correction For details on acquisition and saving of the contents of the shading correction refer to Section 2 1 6 Registering Items Relating to Camera Photometry REG1 Menu in Chapter 6 8 Confirming exposure information There are two methods to confirm the exposure information After confirmation adjust the exposure as necessary Confirm that you are within the controllable exposure range with a level meter or an error message Use a level meter or an error message on the upper right corner of the screen to check the drift from the exposure level that the DS L2 has determined to be optimum Because the exposure level considered to be optimum varies with each subject the level determined by the DS L2 should be regarded as an approximate guide The optimum range for the level meter is indicated by a green bar As the exposure becomes excessive it changes color to red In cases of underexposure it becomes a sky blue bar An Exposure Over or Exposure Under message will be displayed if overexposure or underexposure is recognized If a red or sky blue bar is displayed during automatic exposure PROG S AE F AE you are outside the exposure controllable range Therefore adjust the amount of incident light by adjusting the luminous energy of the light source or the iris diaphragm or by using an ND filter
155. e updated Select your desired interval time from the pull down menu The display on the preview screen is automatically updated at the interval you set The set interval should be considered as a target interval If you select Off the preview screen will not be automatically updated 4 Quality Set the picture quality of the preview screen The higher the picture quality is the slower the display speed of the preview screen will be Select the quality according to your application Some pictures with certain picture quality cannot be displayed depending on the current camera mode Such pictures cannot be clicked 320 240 Low picture quality thinned out to 1 16 640 480 Middle picture quality thinned out to 1 4 1280 960 High picture quality not thinned out For the quality setting for cameras other than DS Fi1 refer to Section 7 Operation Differences among DS Camera Head Models in Chapter 6 5 E Zoom 6 Direction buttons 7 Sample image E Zoom allows you to turn the electronic zoom on or off When you select the E Zoom check box the direction buttons in 6 and the sample image in 7 are displayed with the preview screen displayed in a 4 fold enlarged view The gray region in the sample image white rectangle shows an enlarged view of what is currently being displayed If you want to change the position of the sample image currently being displayed in an enlarged view click directly in the new position t
156. e white balance cannot be adjusted precisely Be especially careful if you are performing exposure in MANU mode or using a low magnification objective To correct the automatically set white balance use the R con for Gain to adjust the balance 7 Adjusting the picture quality The Advn menu has various functions for adjusting the picture quality Watch the monitor while you adjust the picture quality as necessary Edge enhancement processes SHP3 to SHP5 The image state is controlled by adjusting the edges of the image The default setting is 2 The edges can be adjusted in nine steps from 3 soft 0 OFF to 5 sharp Noise reduction NR ON NR OFF Selecting NR ON helps to reduce noise on the screen resulting in a clear image Lag or tails on moving objects may occur when NR ON is selected When photographing a moving subject or if an increased response during focusing is desired select NR OFF Black level SFT If you want to eliminate flare reflection in blacks on the screen or make blacks on the screen more distinct adjust the black level to the negative side Image level Gain Use this facility when adjustment by COMP or C gn has no effect on the brightness of the image Gain is referenced to Y100 100 and can be fine adjusted in increments of 5 The feature of this facility is that correction can be applied to a frozen image as well as to normal images Be aware that lowering the imag
157. ecified USB 232c conversion adapters for this function Do not connect or disconnect the USB 232C conversion adapter when the DS L2 is on i Use the USB 232C conversion adapter only when the LV150A microscope is connected The system will not function properly if nothing is connected or if instruments other than the LV150A microscope are connected 1 Use a USB 232C conversion adapter to connect an LV150A microscope to the DS L2 2 Turn on the LV150A microscope and then turn on the DS L2 3 To switch to a certain nosepiece open the menu and select the corresponding number Lv NCNT Ij 10 MODULE 1 2 3 4 E 4 After switching nosepieces confirm that the selected number icon is indicated by a red border and that the nosepiece number and the objective information are displayed NOTE Information entered during calibration will be displayed in the information column of the current objective Switching objectives will delete all overlay data with the exception of the XY scale and the scale currently displayed For more information on overlay data refer to Section 3 2 What is an Overlay Switching objectives will also change the XY scale and the scale currently displayed 202 Chapter 7 Care and Maintenance 1 Cleaning the System Care and Maintenance 4 Cleaning the System e Use a soft cloth to wipe away dirt from the DS L2 the remote controller and the cables e Before cleaning the DS camera
158. ed file Pickup JPEG Click the image size you want to download and the latest image in that size will be displayed in a new window 1 When you click any image size to select Save it to disk a screen is displayed for you to select the folder in the PC that you wish to save to When you right click on the displayed image the image can be downloaded into the PC in JPEG format If you select Save As in the displayed menu the screen shown below will be displayed Save in a Nikon z Tl ei C Nikont C Nikon amp L Nikon10 C Nikon Lal Nikon2 CJ Nikon8 C Nikon3 C Nikon8 File name outipea Save as type JPEG ipa Cancel Li 114 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 2 Select the folder in the PC where you want to save the image file Enter a file name as necessary and click the Save button When you click the Save button the image that is in the DS L2 is downloaded into the PC Because the latest image is downloaded it may not always the same as the preview screen Note that the Jpeg Quality pull down menu in the Pickup JPEG column allows you to select either Fine high picture quality Normal medium picture quality or Basic low picture quality as the picture quality of the image when you save it The higher the picture quality the larger the file size will be 3 Interval Timer Set an interval at which the preview screen is to b
159. ed separately Power On Startup settings Network settings Refer to settings Network settings Section 4 2 2 Setting Essential Network Settings File settings Items Network Settings in File Settings Other environment settings Chapter 6 Additional Registration of game pad Other settings Refer to Section Settings Return to window to return to the upper 1 4 Initial Settings in Chapter HID Mapper layer 6 Game Pad Shortcut Buttons for frequently used functions Refer to Section 1 3 3 Using and Registering the Shortcut Menu in Chapter 6 Tool Bar Measurement tools for measuring distance Refer to Section 3 Performing between 2 points circles etc Measurements or Entering Information TOOL Menu in Chapter 6 Status Bar Exposure information date cursor position Refer to 1 3 4 Status Bar in menu display ON OFF button and others Chapter 6 Common icons on CAM VIEW REC TOOL and MIC menus The icons listed below can be used in all menu screens E Resizes the displayed menu Closes the menu Ill Freezes the image F y l A Electronic zoom 28 Chapter 6 Basic Operation Method SETU P 1 Preparations Before Photographing 1 3 2 Method of Displaying a Menu Follow the procedure described below to show or hide a menu or to switch between menus Using the mouse Right clicking with the mouse displays a menu The specific menu displayed depends on the mouse pointer position on t
160. ed to the custom mode the conditions can be used for other observations repeatedly For the DS L2 seven kinds of photographing conditions can be saved to to c7 Click one from among to on the Adv1 menu to call up the saved photographing conditions In the Info box at the lower of the Adv1 menu details of the conditions can be checked before calling up For the saving method of the custom mode refer to 10 Saving photographing conditions of Section 2 1 3 Fine Adjustment before Photography Advn Menu in Chapter 6 A7 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images 2 Adjusting the white balance Before photographing an image you need to adjust the white balance of the camera using a subject which is evenly white overall When using diascopic illumination for microphotography press while you are photographing a transparent part of the preparation and the white balance will be adjusted When using episcopic illumination for microphotography or using a lens adjust the white balance in the same way by using a white subject For fluorescent photographing we recommend that the white balance be adjusted under normal light conditions before photographing an image If the screen is excessively dark or if clipping occurs adjust the luminous energy of the light source or the iris diaphragm or use an ND filter to obtain the appropriate white balance 3 Adjusting the focus Determine an image composition an
161. ed when you click the icon for the media drive e A similar message is displayed when you click the icon for the CCD image drive e When you connect the DS L2 other USB devices already connected to the PC will immediately become unusable In such a case change the drive letter assigned to the media drive or the CCD image drive The following shows how to make this change 1 Select Start Control Panel Administrative Tools Computer Management Disk Management 2 Right click the volume whose drive letter you want to change and select Change the drive letter and path 3 Click Edit and choose one of unassigned drive letters from the select box or enter a drive letter directly from the keyboard and click OK 4 Click OK in the ensuing confirmation dialog box 166 Chapter 6 Basic Operation Method USB 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 5 3 Using the Media Drive Receiving image files Insert media into the card slot of the DS L2 and display the Explorer screen from the confirmation screen described above You can read out the media Or you can double click the media drive removable disk icon located below Devices with Removable Storage on the My Computer screen to read out the media The image files on the media drive can be retrieved from the media in the same way as files are normally manipulated on a PC NOTE Under the default condit
162. eeessseeeseees 67 2 2 3 Reproducing Images from a Medium VIEW Menu eese 68 2 2 4 Using Dual Window Display to Compare with the Reference Image 70 2 3 Outputting Images Directly to a Printer cee ccccceeceeeseeeeeeeeeeeesseeeeeeaeeeeeaaaeees 71 23A PANUNG AI PEIUS 71 2 3 2 Various Print Related Settings ccccccccsssceccsssceseeseeeceesecseseesseseeesseseessees 71 Performing Measurements or Entering Information TOOL Menu ssesseeees 73 Sal Outline or tie TOOEMGTU sorire sa residue ue ensste uten A 73 Oe Whats anovar ay saien tecnica ieu than teu ente buste tete esee rae ae ce 75 3 3 Entering a Line or a Comment on the Image cccccccseeeeeeeeeeeseeeeeeseeeeeesaaeees 76 S071 Eree TOITm DI AWING s sd te ees tae ceed tcc hs du coh eos 76 932 SUI ene Drawing RE wl cscs cS ae tee Seg 77 3 3 3 Using the Count Marker to Count the Number of Points 78 3 3 4 Entering Textual Comments cccccseecccceeeeeceeseecceeseecseasecseuseesseueeesseneeeseaes 79 3 4 Measuring on the SCreen ccccccccssseecceeseeeceeseecccesseecseueeessaueeessueeessaneeessaaeees 80 3 4 1 Performing Calibration and Saving Values ccccccceeeeeeeeeeeeeeeeeeseeeeenaeeeeeas 80 3 4 2 Calling the Calibration Value seeseseeeeeeeenrnnemm 82 3 4 3 Measuring the Distance between Two POINts
163. efile openitweh Sd 9 Save it to disk Always show this dialog before handling Files of this type 133 N 00 RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network e After selecting an appropriate directory to save the file click Save to start downloading it Enter name of file to save to my Documents 3 Mv Computer amp my Network Places My Computer My Network File name jre 1 5 0 O9 windows i586 p ifted Places Save as type exe Once the file has been downloaded double click it to start installation The J2SE installation screen is displayed From this point follow the same procedure described previously for Windows and Firefox 3 Disable personal firewall blocking For details refer to Section 4 3 3 ActiveX Settings For Mac OS and Safari 1 Change the browser settings e Select Safari gt Preferences icu File Edit View History Bookmarks About Safari p Report Bugs to Apple Preferences a Block Pop Up Windows dK Window Help Private Browsing Reset Safari Empty Cache Services Hide Safari Hide Others Show All Quit Safari 134 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network e Click Security If the Security icon is not displayed click the button in the upper right corner e Select Enable plug ins e Select Enable Java e Select Enable JavaScript e00 Security D EELA
164. eleting images 249 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network Other settings Network settings and confirmation Network settings and confirmation can also be accomplished by selecting Macintosh HD Applications Utilities Network Utility e The Info tab Shows the IP address and other information e The Ping tab Sends a ping command To use a function that is equivalent to the command prompt in Windows select Macintosh HD Applications Utilities Terminal to display the Terminal screen AASE NETWORK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 4 6 2 Setting External Servers of the DS L2 Network Settings Consult the network administrator in your organization for details on the FTP server If you are going to connect the DS L2 to a LAN you need to enable it for network operation and set its own address Setting the FTP client function To set the FTP client function of the DS L2 select Network Settings from the SETUP menu to call up the Network Settings screen shown below Power0n Set NetWork Set F ile Set Addit ional zl HID Mapper Ret to Window NetWork Settings Enable NetWork Read Only FIP WEB disable Write Control D H C P Auto get an IP Internal Server ip address 192 168 020 002 SubNet Mask 255 255 255 000
165. en the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to select it n Press the cu icon to save a measurement result in CSV format The destination depends on the settings on the REC menu Press the X icon to select and delete a measurement result i Press the E icon to update information being displayed 84 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu 3 4 5 Measuring Angles You can measure an angle between two lines on the screen and save the measurement lines lines and angle for this measurement as part of the overlay for the image 1 Press T Bar The tool bar shown below will be displayed Select the X icon NOTE Check if the icon is selected 2 Press a color icon red blue green black white or yellow to set the color of the measurement lines 3 Draw two lines tangential to the object to form the angle to be measured Click the mouse button at the start point of the line and click the mouse button at the end point to draw a line The angle formed by the lines will be displayed in the vicinity of the two lines If the angle cannot be measured NG will be disolayed To redraw an angle use L or to cancel or redo your most recent action 4 Press the icon or on the Tool menu to close the TOOL Bar NOTE rj 1 1 Press the icon to hide all of the measurement results being displayed Fj Pres
166. en the switch is ON closed DS L2 Required pulse width 1 ms or more The TTL or LVTTL level signal can be input to Pin 1 and 2 When supplying a trigger signal from external devices Functions Pin 2 Trigger input Pin 1 Read out transfer timing signal output When the DS Qi1Mc is connected capture is possible using trigger signals from external devices Images are captured in response to the trigger signal supplied Additionally in this mode a read out transfer timing signal is sent from Pin 2 For instructions on capture based on trigger signal timing refer to the section on DS Qi1Mc in Chapter 6 Section 7 Operation Differences among DS Camera Head Models 3 3 V 4 7k Q 100 Q Pin 2 E Activated when the switch is ON closed Required pulse width 1 ms or more NP NN i Pin 3 DS L2 External input device The TTL or LVTTL level signal can also be supplied through Pin 1 17 Chapter 4 Connecting and Installing the Equipment 3 Connection Methods When sending a sync signal to an external device Functions Pin 1 or 2 For capture sync output Can be used for synchronized lighting of a stroboscope when capturing images with the DS L2 Capture sync output is TTL level output The output pulse width is approximately 20 ms External input devices that satisfy the following electrical characteristics can be connected Refer to the following diagram e he input buffe
167. enerated from the DS L2 and its peripherals Chapter 2 Peripheral Equipment 11 Personal computer PC You will need a personal computer to operate the DS L2 from a remote location or to display or process the DS L2 image data on the PC There are two methods to connect between the DS L2 and the PC 1 2 The PC controls the DS L2 from a remote location or captures photographed images via network The PC and the DS L2 are connected with a USB cable to capture photographed images or to read or write data in a medium that is inserted in the DS L2 The DS L2 is connected to the PC as a removable medium Connect a PC and the DS L2 with a USB interface to control the DS L2 and to display live images or capture photographed images with the specified application software on a PC The operating environment of the PC depends on the connection method 1 Network connection Refer to Section 4 2 Network Settings in Chapter 6 2 USB connection connected as a removable media Applicable model Windows PC OS Windows 2000 Service Pack 3 or higher Windows XP SP 2 Vista 3 USB connection using the specified application software PC unit Item Requirement Type Windows PC equipped with USB 2 0 CPU Equivalent to Pentium 4 3 2 GHz or higher Memory 1 GB or more Hard disc 100 MB of free hard disk space for installation 300 MB of free disk space for program execution Video 1280x1024 pixels High Color mode or better True Color
168. ent action Press the JX icon or on the Tool menu to close the TOOL Bar NOTE Be sure to use these functions in the state where values are labeled with the units nm um mm or cm If a unit other than these is being displayed set calibration values again 7 If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for Control License Card sold separately i If you specify points to cause lines to cross the area may not be measured correctly rj Press the icon to hide all of the measurement results being displayed Fi Press the icon under this state to display them again i You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu li You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu i Press the CLR icon to erase a measurement point existing on the image When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to erase it Press the ROL icon to use a measurement point existing
169. equipment falls under the category of electric shock protection class always make sure it is grounded to a protective ground terminal When the power cord is lost or damaged contact your nearest Nikon representative For regions where the power supply is 100 to 120 VAC Use a UL listed detachable power cord set 3 conductor grounding Type SVT No 18 AWG 3m long maximum rated at 125 VAC minimum For regions where the power supply is 220 to 240 VAC Use only an EU EN approved three conductor power cord set 3 conductor grounding Type HO5VV F 3m long maximum rated at 250 VAC minimum 5 Handling e To prevent malfunctions or overheating due to short circuits do not allow this equipment or any other device used with it to become wet Never insert any foreign objects or materials into the equipment If the equipment gets wet or if foreign matter e g a scrap of paper gets inside the equipment turn off the DS L2 s POWER switch and unplug the power cord from the outlet Then contact your nearest Nikon representative Do not bend or twist the cables with undue force Doing so may damage the cables and result in fire e Do not place a cloth or paper on the DS L2 or DS AC adapter Do not block the ventilation hole of the DS L2 Doing so may cause heat to collect inside the equipment resulting in fire 6 Precautions for disposal This equipment uses a fluorescent lamp containing mercury When disposing of this equipment entrust the di
170. er end to the CAMERA OUT connector on the DS camera head Squeeze the locking tabs on the connector as you plug in the connector When the connector is plugged in all the way the locking tabs return to their original positions locking the connector in place Mounting the DS camera head on a microscope 1 Remove the C mount cap from the C mount on the DS camera head To CAMERA connector 2 Make sure there is no dust on the C mount adapter on the DS L2 If there is use an air blower or similar means to remove it DS camera head C mount 3 Screw the C mount adapter into the C mount on the DS camera head C mount adapter Screw it in as far as it will go 4 Insert the C mount adapter into the vertical tube of the microscope Use the clamping screw on the vertical tube to secure the C mount adapter in place Vertical tube of microscope You can also attach a relay lens We recommend the x0 7 relay lens for the DS Fi1 DS Qi1Mc DS 5M and DS 5Mc We recommend the x0 55 relay lens for the DS 2Mv DS 2MBW and DS 2MBWc When you have mounted the DS camera head on a microscope be sure to read Chapter 5 Microscope Adjustment as well Installing a third party C mount lens on the DS camera head Select a mountable lens see Chapter 2 Peripheral Equipment then screw it into the C mount Note that the image quality produced by this product is tuned for microphotography The tripod mount on the DS camera head can also
171. er of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for i Control License Card sold separately 95 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu Changing the scale length or color The scale length and color can be changed 1 Move the cursor over the displayed scale and left click the mouse 2 A menu for changing the scale length and color appears EE ons HERE ENT aon E 3 To change the length enter the desired value using the software keyboard and then press Ent 4 To change the color select one from the six colors red green yellow blue white and black NOTE The unit cannot be changed If you change the scale length to a value out of the display range the scale will be displayed as a length multiplied or divided by a factor of 10 resulting in 10x 100x or 1 10 1 100 O6 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu 3 5 4 Using XY Scales to Measure the Dimensions of the Subject I I H H d HH He H H H H2 H H H H 10mm 0 Oih ot By displaying XY scales on the screen you can measure the approximate dimensions of the subject Because the X and Y scales can be moved independently you can move the s
172. ers of the DS L2 Network Settings 144 4 6 3 Saving Image Files in the FTP Servel cccccccsecceeceeeeeeeeeeeeeeeeeeesaeeeeeeeeeeeeas 146 4 6 3 1 Using the CAPTURE Button to Save Image Files 146 4 6 3 2 Using the CAM Menu to Save Image Fil S cccccecsseeeeeeeeeeeeeeeeeeeeeeeeeas 147 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 149 5 1 Recommended Operating EnvirOnMent ccccccccseeceeceeeeeeeseeeesaeeeeaeeeeenees 149 5 2 Connecting the DS L2 and a PG tee apo nare utr eter fd estudian 151 5 3 Using the DS L2 in Windows 2000 sssseeesseeneeeen nnm 152 5 3 1 Installing the Device DTIVOLr iiie rti rine anna o en eon nn E rn one su en e 152 5 3 2 Outline of the Disk Drive cccccccccccssececceeececceeeeceeesecseeeesseseeesseeeessaneeenes 153 bio 9 Using ihe Medla DING open us E mass pun dup U AUN al ca dup Mods 155 5 3 4 Using tlie CCD Image Drive sexiest siot sitet atero ec ct exu E reet veo onsite aa 156 5 3 5 Removing the DS L2 from the PC eese nnn 158 5 4 Using the DS L2 in Windows XP sssesssseeeenneennn nennen 159 5 4 1 Installing the Device DIivel cccccccseecesssceseseeeeeeeeceueeseueeseeeeesseeeneueesseetens 159 5 4 2 Outline of the Disk Drive eessseesssssesssseeeeneenennnnm nemen 160
173. ervice and Windows starts the installation All of the following items can be set from Control Panel Administrative Tools Computer Management Adding a user Create a new user account 1 Select Computer Management Local System Tools Local Users and Groups 2 Right click on User to select New User 3 Enter a user ID and a password To ensure that the same information has been set for External Server in Network Settings of the DS L2 enter the user ID and the password within ten alphanumeric characters in lower or uppercase Deselect User must change password at next login check box NOTE You can also create accounts and set passwords from Control Panel Users and Passwords or User Accounts for XP Specifying the home directory and enabling write to it Enable image files to be written from the DS L2 to the PC by FTP 1 Select Computer Management Local Services and Applications Internet Information FTP Sites 2 Right click Preset FTP Sites and select the Home Directory tab in the ensuing Properties dialog box 3 In Local Path for Directory at FTP Site specify the home directory or default home directory to which image files are to be transferred Also select the Write check box to enable writing to the home directory When image files are transferred the FTP folder specified on the DS L2 side is created at a location below this home directory an
174. exactly the whole image as big as possible Adjusts the zoom factor to view the current image on the 1 1 scale Increases the zoom factor of the current image by one level Decreases the zoom factor of the current image by one level Enables to zoom the image within a range of 10 to 1200 Click the arrow at the right of the button to select a zoom magnification Chapter 6 Basic Operation Method USB 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function 5 Tools 6 Working with camera iow Displays a scale in the bottom right Eo Displays the live image in the picture window corner of the image Click the arrow at scale the right of the button to open the pop up Live menu and then change the magnification T Freezes and grabs the live camera image into of the scale 2 2 5 4 10 20 40 50 60 the picture window as the Frozen document 100 150 times Freeze A A number of annotation objects can be inserted into the image Click the arrow Annotate at the right of the button to open the pop up menu and then select the object to be inserted from the line arrow text rectangle or circle mode This command captures Live or Frozen image to the application window It creates a new document called Captured This command captures Live or Frozen image and saves it automatically and names it according to the current settings File menu gt Options a An intensity
175. f scene menus SXGA XGA LCD E pc I F Active XGA LCD ON Sets position on which the main menu is displayed and its size LCD Back Light JE 5 Display YGA Color Adjust Sets image display Sets color balance of Mirror Normal image display Default Sets mirror display of DS 12 Ver 320m 0601 0298 070720 image Shows version information for firmware When you have finished setting each item press SAVE to save the data that has been set Or press Default to restore data to default values Power On Settings No 1 3 You can register one of the Power On Settings three kinds and call it e Registration of Power On Settings After selecting Power On Settings No register setting details for each No When you press SAVE the setting details are saved as a selected No e Calling of Power On Settings After selecting Power On Settings No you wish to use press SAVE From this time forward the DS L2 is started up based on the setting details for the selected No CAM Custom No 0 turn off 1 7 The set values for the subject objective etc can be registered in custom mode Here you can specify the number for the custom mode to be used at startup When you select 0 turn off the DS L2 is started up with the number specified at the time of the previous power off NOTE The contents of the custom mode can be set from the CAM REG1 menu 41 SETUP Chapter 6 Basic Operation Metho
176. fer to Section 4 3 3 ActiveX Settings NOTE If ROOMA is not displayed make sure any personal firewall of the operating system or antivirus software is not preventing it NOTE If installation of the Java plug in in Windows fails access http www java com en download manual jsp and download the file manually Try installing the file after momentarily saving it to disk This download URL is subject to change The Java plug in is installed by default in Mac OS There is no need to install it 136 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network Oo 44 Using FTP Commands to Operate the DS L2 You can run FTP commands on your PC to get images from the DS L2 NOTE Always be sure to consult the network administrator in your organization before executing FTP commands Your organization s network may have been set up in a unique manner so invalid operations might cause a problem on your network 4 4 1 Using FTP Commands to Get DS L2 Image Files from a PC 1 Enter the IP address of the DS L2 from the MS DOS prompt command prompt of Windows to connect to the DS L2 Example FTP 192 168 10 2 2 Execute the Is command to search for a directory named RAMIBOX 3 Execute the cd RAMIBOX command The following virtual file names will be displayed e IMG bmp e IMG jpg e IMG tif Here represents dimensional values such as 1680 or 1280 indicating image size The time of day for the
177. fferences among DS Camera Head Models DS 5M DS 5Mc ARAA CSch Eu 7 BAIN CHRM HUE SFT When DS 5M is connected the Cooled display that represents the cooled camera is not displayed EIE m T O oF Adv1 menu Adv2 menu Features of display modes An image area of 1280 960 pixels is formatted for the monitor resolution The image is displayed at actual size 1280 960 SXGA or reduced 1024 768 XGA The frame rate is 7 5 fps This display mode is useful when good performance with moving subjects is required Progressive display modes eliminate color shifts and provide natural images even with moving subjects We recommend using this display mode as the general display mode 1 3Mp CCD Mode 1 An image area of 1280 960 pixels is formatted for the monitor resolution The frame rate is 6 7 fps As two pixels are added respectively in the vertical and horizontal directions to make one pixel this mode is four times more sensitive to light than other modes making it particularly useful for dark subjects Interlaced display modes may cause color shifts with moving subjects 1 3Mi CCD Mode 2 An image area of 2560 1920 pixels is formatted for the monitor resolution The frame rate is 3 8 fps This display mode is useful when viewing images in great detail We also recommend this mode when making long exposures Interlaced display modes may cause color shifts with moving subjects 5Mi CCD
178. fps C Scan 15 fps C Scan Number of 1600 x 1200 2560 x 1920 recording pixels 800 x 600 1280 x 960 400 x 300 640 x 480 Quantization 12 bits Sensitivit Equivalent t peeve pos e z Equivalent to ISO 350 Equivalent to ISO 64 Settings Tone 3 camera sensitivity x1 Lens mount C mount Supported lenses Protruding no more than 10 mm from the mount surface into the camera Image circle 68 9 mm Image circle 611 mm Dimensions 91 W x 76 D x 41 H mm Weight 260 g 290 g 260 g 290 g Fixing tripod vd i Tripod screw for cameras mount Bod Whit Whi Black ody color Ite Ite ac White Black with logo with logo with logo CCD cooling Peltier cooling Peltier cooling 1 The CCD mode is indicated in parentheses EF DS Remote Controller DS RC External dimensions 56 W x 68 D x 21 H mm Weight 110g 218 Chapter 9 Technical Specifications 4 DS AC Adapter 4 DS AC Adapter Model name Manufacturer Rated input voltage Rated output voltage External dimensions Weight Conforming standards F10453C F1650K AD 1260B EPS Electricity Power Source F10453C F1650K Medi Power Electronics Inc Manufacturer Powertron Technology Co Ltd AD 1260B 100 240 VAC 50 60 Hz 1 2 A maximum 12 VDC 5 3 75 A max F10453C 3 5 A max F1650K 5 0 A max AD 1260B 117 L x 53 W x 30 H mm F10453C 121 L x 60 W x 35 H mm F1650k 121 L x 62
179. from that in other modes Press the DF FL scene mode button to display the high sensitive image display mode and adjust the focus To adjust the focus press Focus button Right click P Focus button to increase the camera gain so that the exposure time will not be long After adjusting the exposure live images are displayed with the exposure conditions remained Left click Focus button to set the manual mode NOTE When the subject is dark amount of noise increases After adjusting the exposure use EE exposure compensation button to adjust the brightness of live images When clicking the exopsure compensation button the button design is changed to The brightness of live images can be adjusted by clicking the button After focusing press exposure start button and the DS L2 starts automatic exposure When finished a screen is displayed with a frozen image NOTE v is displayed when exposure time is longer than 6 seconds Right click stops photographing Check the screen and save the image with Capt button or ey Media save button Additionally the image can be printed with A Pictbridge button exposure compensation button is also used to adjust the brightness of photographing images After changing the photographing conditions perform the exposure again with exopsure start button To return to the live image from this status press a Focus button To change the mode from the D
180. ftware according to the following procedure 171 USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function Installation procedures Click the F Package on the installation screen Click the Select All button to choose all the cameras Then click the Install button 2 Browse CD The Setup screen appears Then click the Next gt button NIS Elements F 2 20 Setup Felcome to the InstallShield Wizard for NIS Elements F 2 20 Press the Next button to start the installation You can press the Cancel button now if you do not want to install the NIS Ele ments F 2 20 Setup Cameras Cameras Cameras j Nikon DS U1 Nikon DS U2 Nikon DXM1200 F Nikon DXM1200C Nikon DS 10M Nikon DQC FS Click here ik iste The Completion screen appears Then click the Finish button NIS Elements F 2 20 Setup InstallShield Fizard Complete The InstallShield Wizard has successful ly installed NIS Elements F 2 20 Click Finish to exit the wizard NIS Elements F 2 20 at this time Click here www laboratory imaging com m PT EMT v Paunch NIS Elements F 2 20 Click here Cancel The Choose Destination screen appears Click the Browse button when the folder for installation needs to be changed And then click the Next gt button NIS Elements F 2 20 Setup Choose Destination
181. ge 4 Media Save shortcut key records an image to the selected Medium FTP Saver Save shortcut key saves an image via the FTP 38 Chapter 6 Basic Operation Method S EIU P 1 Preparations Before Photographing PictBridge shortcut key outputs an image with the PictBridge interface mM AE Unlock Lock shortcut key switches Unlock Lock of the AE White Balance shortcut key performs the white balance adjustment Freeze shortcut key freezes the screen 3 Zoom Up shortcut key zooms in the screen Zoom Down shortcut key zooms out the screen CMP UP shortcut key increase the CMP value of the camera CMP Down shortcut key decrease the CMP value of the camera ae OS Test Screen operation check window The light up position on the screen indicates which key is assigned HID registration method As a first step see the Cautions on Setting the HID in this section 1 Connect the HID Game Pad and start up the DS L2 2 Display the Menu and select the SETUP tag to show the SETUP menu On the SETUP menu select the HID Mapper to show the settings screen Do not touch the HID buttons when pressing the HID Mapper 3 Check that the Maker ID now and Product ID now are indicated on the settings screen and that the Data value changes when pressing the HID buttons NOTE When the Maker ID now and Product ID now are not indicated restart the DS L2 and perform the procedure 1 and 2 If those IDs ar
182. h part of the HID Mapper screen is named as follows PowerOn Set NetWork Set File Set Additional HID Mapper Ret to Window HID Game Pad Mapper Save Number Maker Product ID now saved Saved ES Data TFTFTFTF FODO00 Basic Keys Short Cuts U t H S ES D V ENEEI r s B Le RTA R b p t EI ENT EN WB WB EM wor NENU EN rz M al Test Screen U ENT MOF R M S F S PRT AE WB D Z u Z D C U C D Clear All FZ D Clear 766 None 0001 None Save Number window Maker ID Product ID window HID output data window Dm Function registration window Function button Operation check window After registering the HID according to HID Registration Procedure in this section press SAVE to save data To clear the selected data in the memory of the checked Save Number press Clear ALL 37 S EIU P Chapter 6 Basic Operation Method 1 Preparations Before Photographing Save Number Specify the memory number and enable the registered data to be read out Up to three types of HIDs can be registered NOTE AHID can be registered to only one memory area not to two or three memory areas Additionally two HIDs if they are the same kind are registered to only one memory area Maker ID now saved The Maker ID registered in the HID appears The ID at left shows the Maker ID of the HID in operation here
183. hapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 2 Install the Java plug in If a Java plug in to run Java applets is not installed you will be requested to install a Java plug in the first time you access ROOMA Follow the displayed installation instructions e Click the image display area Image Window with Cotrol Pad Mozilla Firefox File Edit view Go Bookmarks Tools Help Q D B amp B OQ teurize s1 30 36 at1 hem G Customize Links Free Hotmail Windows Media Windows Jk Additional plugins are required to display all the media on this page B 2 Control Pad Interval Timer Quality 320240 640480 Click here to download plugin e Click Next Plugin Finder Service Available Plugin Downloads The following plugins are available Java Runtime Environment Some plugins may require additional information From you during installation Press Next to install these plugins 127 NETWORK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network The J2SE installation screen is displayed Select Typical setup and click Accept ig J2SE Runtime Environment 5 0 Update 9 License License 4greement Please read the Following license agreement carefully Sun Microsystems Inc Binary Code License Agreement for the JAYA 2 PLATFORM STANDARD EDITION RUNTIME ENVIRONMENT 5 0 SUN MICROSYSTEMS INC SUM IS WILLING TO
184. hase a DVI cable we recommend selecting a cable length of 3 meters or shorter to prevent quality deterioration due to the noise generated from peripheral equipment 2 USB mouse Use a USB mouse for various menu settings and operations You also need the mouse to access functions available only by the mouse e g screen scroll while using the electronic zoom TOOL functions such as length measurement or the pen drawing function or histograms Please use a model whose operation is verified by Nikon Not every USB mouse is compatible with the DS L2 3 USB keyboard Although you can enter photographed data and comments using a mouse it is easier to enter such information with a keyboard Please use a model whose operation is verified by Nikon Not every USB keyboard is compatible with the DS L2 Chapter 2 Peripheral Equipment 4 USB hub You will need a USB hub to use a mouse keyboard printer or USB memory at the same time Please use a model whose operation is verified by Nikon Not every USB hub is compatible with the DS L2 Only one hub can be connected to the DS L2 If your keyboard is equipped with a USB hub use this hub 5 USB printer Photographed images can be directly printed by connecting the equipment to a printer that supports PictBridge 6 USB memory A USB memory allows image data to be recorded or reproduced Please use a model whose operation is verified by Nikon Not every USB memory is co
185. hat you want to enlarge or click one of the four up down left right direction buttons If you deselect the E Zoom check box the E Zoom function is turned off and the direction buttons and the sample image disappear with the preview screen displayed in normal size If high picture quality 1280 x 960 is selected for the picture quality of the preview screen a middle quality 640 x 480 image is displayed in an enlarged view 8 Location The DS L2 installation information etc that has been set by Telnet commands is displayed here Settings by Telnet commands Connect from an external PC to the DS L2 by Telnet Refer to Section 4 5 Using Telnet Commands to Operate on the DS L2 in Chapter 6 Execute the command w_LCA_string 32 characters and information to Location can be written The string can be entered using up to 32 ASCII characters which when displayed appear in uppercase letters 115 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network The information is displayed on the screen when you restart the DS L2 by turning it off briefly and then on again 9 Preview screen Use this screen to check the image in the DS L2 before downloading it into the PC When you change the size of the window the size of the preview screen changes accordingly NOTE When the camera mode is changed on the menu while the preview screen is being displayed the screen may be temporarily distorted but will
186. he CCD source setting is Source Now and the CCD mode is 5Mi select 2560 1920 1280 960 or 640 480 for the setting of size of saved images When the CCD source setting is Source Now and the CCD mode is C Scan the setting of size of saved images is fixed to 1280 480 When the CCD source setting is Source Full the CCD mode is automatically switched during image acquisition regardless of the current CCD mode Thus you can select 2560 1920 1280 960 or 640 480 for the setting of size of saved images The following table gives the resulting file sizes for various image file settings Since actual file sizes of JPEG images can vary widely from subject to subject the JPEG values given here are intended to serve only as a guide File Type JPEG JPEG JPEG JPEG TIFF E ECCE EE Draft 2560 1920 14MB 1 19MB 12MB 2MB 960KB 14MB 1280 960 3 60MB ie 480KB 320KB 240KB 3 6MB 640 480 900KB 260KB 120KB 80KB 60KB 900KB The size of saved images can be set in the same manner through the operation on the WEB screen operation by the FTP command operation by the Telnet command and operation with USB connection 194 Chapter 6 Basic Operation Method CAMERA VARIETY 7 Operation Differences among DS Camera Head Models Bril Bos SJ Boo SX CS PROB epu ZE of We AEC an rio 8100 5100 EISE 00 Mills SFT SO_OFF NR OFF SHP 0 COLOR oiset DS 2Mv Adv1 menu PROB
187. he DS L2 The latest image files that have been shot by the DS L2 are stored in the DS L21MG folder and these files can be downloaded to the PC NOTE Because the CCD image drive is read only no image files can be sent from the PC to the DS L2 The image files stored in the DS L21MG folder have size set in the file settings selected from File Settings of the DS L2 SETUP menu Example IMG1280 bmp IMG1600 bmp NOTE To capture files at maximum resolution you must set the correct parameters for size and the correct display mode The display mode also needs to be set to maximum resolution Setting the display mode to a lower resolution will result in image captures at the lower resolution The image files stored in the DS L21MG folder are updated once every 5 seconds Be aware that if files are read out at intervals shorter than 5 seconds you will not always get the latest image To confirm the time of day at which image files have been updated follow the procedure described below 168 Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function Confirming the updated date time of image files 1 On the Computer screen display image file icons Then press the F5 key to update the image files gt Computer NIKONIMG G gt DS LZIMG LT Organize EE Views Date taken Tags is IMG1280 E Documents E Pictures i Music lai Recently Changed Ls Se
188. he camera settings for exposure time and camera gain the image is frozen e Select V ANI for bulb photography in which the exposure begins at the falling edge of the trigger signal and ends at the rising edge of the trigger signal e Images are frozen in either case To save the images use the capture feature of the DS L2 However note that frozen images will CROP Arpa CCD Mode 4 3 12864728 Ex Trigger Mode be cleared before saving if you change the CCD mode or play back gon mU images using the VIEW menu because an external trigger will be CCD Cooling Temp received EE e n external trigger capture mode video images cannot be monitored m 10 C 60 RHmax until trigger signals are received Also normal images are not saved if images are captured before a trigger signal is received e Noise reduction is automatically deactivated In case of connecting to a Nikon inverted microscope Ti and using the external trigger capture mode be sure to turn off the DS L2 before connecting the trigger port of Ti to the EXT I O connector of the DS L2 Attempting to make this connection with power on may display an error on the screen of the DS L2 189 CAM ERA VARI ETY Chapter 6 Basic Operation Method 7 Operation Differences among DS Camera Head Models e There is a time lag of up to 0 7 ms between reception of the trigger signal and exposure e Once a trigger signal is received the next signal is not accepted until freezing
189. he microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for i Control License Card sold separately 3 4 3 Measuring the Distance between Two Points 1 idum You can measure the distance between two specified points on an image The measurement line you used for this measurement can be saved to the overlay for that image NOTE You need a mouse to use this function This function cannot be used unless you first perform calibration or call up the saved calibration value Because this is a simple function for measurement its accuracy is not high 82 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu Press T Bar The tool bar shown below will be displayed Select the icon NOTE Check if the A icon is selected Press the red blue green black white or yellow color icon to determine the color of the measurement line Draw a measurement line Click the mouse button at the start point of the object to be measured and click the mouse button at the end point to draw a line measurement line The length of the measurement line will
190. he screen when you right click e Top 1 3th of the screen Main menu e Middle of the screen Main menu and shortcut menu e Bottom 1 3th of the screen Shortcut menu Switching menus on the main menu window is performed in the following procedures 1 Move the cursor to a desired tag The part around the tag is highlighted in yellow 2 Click with the mouse To close the menu window right click on a part other than the menu window You can close it by clicking on the X button The status bar is displayed at the time of the initial menu display after the DS L2 is started To hide the status bar move the cursor to the status bar and right click on it Using the keyboard Pressing a function key on the keyboard displays one of the following menus e F1 CAM menu e F2 VIEW menu e F3 REC menu e F4 TOOL menu e F6 MIC menu To change menus select your desired tag on the current menu with a cursor button and hit the Enter key on the keyboard to confirm To close the menu screen re press the function key 20 S EIU P Chapter 6 Basic Operation Method 1 Preparations Before Photographing 1 3 3 Using and Registering the Shortcut Menu With the shortcut menu you can use the DS L2 functions by one click operation Move the cursor to a shortcut button The part around the button is highlighted in yellow Subsequently click on it with the mouse to use the function assigned to the button For details on how to photograph an image
191. he select box or enter a drive letter directly from the keyboard and click OK Click OK in the ensuing confirmation dialog box 153 USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function How to discriminate between the media drive and the CCD image drive Right click any removable disk icon on the My Computer screen to display its Properties dialog box This dialog box helps you identify the drive assigned to that removable disk In the case of the CCD image drive for example it can be identified based on the volume label NIKONIMG and its capacity 16 0 MB as shown below Removable Disk H Properties E x General Tools Hardware Sharing Label MIKONIMG Type Removable Disk File system FAT E Used space 366 640 bytes 360 KB E Free space 16 410 624 bytes 15 5 MB Capacity 16 7 79 264 bytes 16 0 MB Drive H TCNEPTNN NOTE Note that because this drive is write protected its volume label cannot be altered 154 5 Using the DS L2 when Connected to a PC by a USB Cable 1 5 3 3 Chapter 6 Basic Operation Method Using the Media Drive Receiving image files USB Mass Storage Function Insert media into the card slot of the DS L2 and double click the media drive removable disk icon on the My Computer screen to read out the media The image files on the media drive can be retrieved from the media in the
192. icroscope LV150A LV100DA LV ECON LV AFCON industrial microscope or AZ100M Universal Zoom Microscope DS L2 provides two USB host ports to each of which a different peripheral device can be connected Peripheral devices listed above can be connected to either port EXT I O connector Two channel signals can be input output Select the SETUP menu and Additional Settings to change between input and output When used as an input port the freeze and capture functions can be performed with the DS RC connected The foot switch may also be used When used as an output port synchronized signals are generated when an image is captured thus the strobe light can be flashed during photography and synchronized signals can be output to the microscope When the DS Qi1Mc is connected externally triggered image capture is possible by supplying external trigger signals from this port Refer to 7 Connecting External I O Devices of Section 3 Connection Methods in Chapter 4 Connecting and Installing the Equipment for details on the interface specifications Power switch This is a push switch Press it to turn on the power Press again to release it and turn off the power When the power is on the LED indicator lights up Stand arm Extract the arm to use it See the right figure Power indicator Flashes in green and then lights up when the power is turned on The DS L2 is ready to be used when th
193. ion image files cannot be sent from a PC to a medium because the media drive is read out only Removing media 1 Check to see that the CF card indicator on the DS L2 is turned off Close the menus displayed on the DS L2 2 If any image files have been loaded from the media to the PC close all of the open image files on the PC 3 Right click the media drive removable disk icon on the My Computer screen and select Eject os Sei L7 Organize S Views v LM AutoPlay e Eject 9 Properties System properties gl Uninstall or change a program we Map network drive fe Open Contro Panel Name Type 2 Total Size Free Space Hard Disk Drives 2 IE Documents Local Disk C Local Disk D E Pictures gt I Music 46 9 GB free of 58 5 GE SUN 00 3 GB free of 90 4 GE jg Recently Changed Devices with Removable Storage 4 EB searches Ld S54 Floppy Disk Drive A DVD RW Drive E Dp E za NIKONIMG G jg Public Ed e Open Explore Search Open AutoPlay Share Open as Portable Device Format Eject Cut Copy Create Shortcut Rename Properties 4 Remove the media manually from the DS L2 167 USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 5 4 Using the CCD Image Drive Double click the CCD image drive removable disk icon on the My Computer screen to access the internal DS L21MG folder of t
194. ion 2 1 6 Registering Items Relating to Camera Photometry REG1 Menu in Chapter 6 6 Adjusting the white balance Before photographing an image you need to adjust the white balance of the camera using a subject that has even whites 1 When using diascopic illumination for microphotography photograph a transparent part of the preparation When using episcopic illumination for microphotograph or when using a lens adjust the white balance with a white subject For fluorescent photography we recommend that the white balance be adjusted under normal light conditions before photographing an image 2 Next adjust the exposure It would be ideal if the amount of incident light can be adjusted in such a way that the shutter speed falls within a range of about 1 8 to 1 250 seconds To adjust the amount of incident light adjust the luminous energy of the light source or the iris diaphragm or use an ND filter 3 Press while in this state and the white balance will be automatically adjusted The white balance can also be adjusted from a frozen image 1 While photographing an evenly white subject press Ill freeze button to freeze the display 2 Press while in this state and the white balance will be automatically adjusted 57 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images 3 Press Il freeze button again to release the frozen display thus completing your settings In case of over or underexposure th
195. ion 2 2 3 Reproducing Images from a Medium VIEW Menu in Chapter 6 e Writing to a medium Under the default condition formatting a medium writing files and deleting files in the media inserted to the DS L2 are disabled for the mass storage function of the DS L2 Telnet command enables those operations of the mass storage function When those operations are enabled do not use functions other than the mass storage function to format write files or delete files becuaseimages saved in the CF card may be damaged To use functions other than the mass storage function disable the operations of the mass storage function with Telnet command Details of the commands are as follows w MSWTON command Permits writing into CF with the USB mass storage function w MSWTOF command Forbids writing into CF with the USB mass storage function This condition is maintained even when the power is cycled 149 USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function NOTE a Media formatted in other than NTFS may not be correctly formatted in FAT16 depending on the formats used If so reformat media in FAT12 FAT 16 or FAT32 on your computer before use Format large media over 2 GB on your computer before use You cannot access the USB memory from the PC in a state where the USB memory is connected to the USB host port of the DS L2 When you wish to read write from to the USB
196. is selected you cannot write to the internal files Unselect this check box to control the DS L2 and write to internal files via a network connection If you only want to get images from the DS L2 leave this check box selected Internal Server Set the IP address subnet mask user ID and password of the DS L2 Refer to Settings for improved security described below for details on how to set a password e When automatically acquiring an IP address D H C P Auto get an IP IP Address Automatically set after restarting Subnet Mask Automatically set after restarting NOTE The DS L2 takes some time after it restarts before the addresses are displayed e When setting a fixed IP address D H C P Auto get an IP IP Address 192 168 010 002 Input example Subnet Mask 255 255 000 000 Input example If any entry in the IP address consists of two digits or one digit add 0 or OO at the beginning so that entry will consist of three digits 8 8 B Click the input box and enter an address value If you missed entering any address value hold down on the screen keyboard while you skip to the last entry of the address then reenter the correct value Once you have finished with all the settings press SAVE and then restart the DS L2 To revert to the initially set value press Default and then SAVE and restart the DS L2 Settings for improved sec
197. isplay a menu and select the SETUP tag on it to enter the SETUP menu Select Power On Settings from the SETUP menu to go to the Power On Settings screen 3 Check if your external monitor complies with VESA DDC2B If so we recommend you put a check mark on DDC I F Active If not do not put a check mark on DDC I F Active 4 Press SXGA XGA LCD With each press the available choices will cycle in the sequence SXGA LCD OFF gt XGA LCD OFF gt XGA LCD ON When you use an external monitor select one of SXGA LCD OFF XGA LCD OFF or XGA LCD ON 5 Colors may not be displayed correctly depending on the external monitor connected to the system If this is the case you can adjust color settings using the color adjustment function for the external monitor or via Display VGA LCD Color Adjustment in the Power On Settings screen 43 S EIU P Chapter 6 Basic Operation Method Preparations Before Photographing 6 Press SAVE to save your settings Turn the DS L2 back on again NOTE If DDC I F Active is in the OFF state no images are displayed on the internal monitor when SXGA or XGA LCD OFF is selected Thus you cannot work with the DS L2 without an external monitor To change settings hold down the CAPTURE button while you turn on the power Keep the CAPTURE button depressed until startup is completed Images will be output temporarily to the internal monitor Select XGA LCD ON in Power On Se
198. ition image files cannot be sent from a PC to a medium because the media drive is read out only Removing media 1 Check to see that the CF card indicator on the DS L2 is turned off Close the menus displayed on the DS L2 2 If any image files have been loaded from the media to the PC close all of the open image files on the PC 3 Right click the media drive removable disk icon on the My Computer screen and select Eject My Computer efx T Fille Edit View Favorites Tools Help o Bak QJ d X Search Folders Fa address Ee Ld Shared Documents ind Administrator s Documents s System Tasks view system information Hard Disk Drives t Add or remove programs G Change a setting meg pe cua Local Disk C cua Local Disk D Other Places i Z cal Local Disk E C egi Local Disk F My Network Places uae ate ug puer L3 My Documents f Shared Documents Devices with Removable Storage G Control Panel A te 7 a 314 Floppy A 17777 xRIPFRE EN G Details q My Computer Em Sen Folder Saggi MATSUZAKI H Seca NIKONIMG 1 Network Drives cuu Psgdata on Psgserv T v 4 Remove the media manually from the DS L2 161 USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 4 4 Using the CCD Image Drive Double click the CCD image drive removable disk icon on the My Computer screen to
199. ize of saved images When the CCD source setting is Source Now and the CCD mode is 640 the setting of size of saved images is fixed to 640 480 When the CCD source setting is Source Now and the CCD mode is 5Mri select 2560 1920 1280 960 or 640 480 for the setting of size of saved images When the CCD source setting is Source Now and the CCD mode is C Scan the setting of size of saved images is fixed to 1280 480 When the CCD source setting is Source Now and the CCD mode is ROI select 1280 960 or 640 480 for the setting of size of saved images When the CCD source setting is Source Full the CCD mode is automatically switched during image acquisition regardless of the current CCD mode Thus you can select 2560 1920 1280 960 or 640 480 for the setting of size of saved images The following table gives the resulting file sizes for various image file settings Since actual file sizes of JPEG images can vary widely from subject to subject the JPEG values given here are intended to serve only as a guide File Type JPEG JPEG JPEG JPEG TIFF Fine Normal Basic Draft 2560 1920 14MB 1 9MB 1 2MB 960KB 14MB 1280 960 3 6MB 1 0MB 480KB 320KB 240KB 3 6MB 1280 480 1 8MB 520KB 240KB 160KB 120KB 1 8MB 640 480 900KB 260KB 120KB 80KB 60KB 900KM The size of saved images can be set in the same manner through the operation on the WEB screen operation by
200. l Instead use a commercially available arm stand or mount that can support the weight of 1 4 kg or heavier Leave a clearance of 100 mm or more from the wall surfaces and periphery Design a layout that allows easy removal of the power cord from the inlet of the AC adapter in the event of an emergency 2 Always turn off power when setting up the equipment or when connecting disconnecting cables To prevent damage or malfunction always turn off the DS L2 s POWER switch when setting up the equipment or when connecting or disconnecting cables 3 Connect only the devices specified to the connectors To prevent damage to the equipment connect only the devices specified to the connectors on the DS L2 the DS camera head or the DS Remote Controller DS RC 4 Cautions on setup installation and storage of the equipment To assemble the product and connect cables check that the power supply for the product is turned off Be careful to avoid pinching your fingers or hands in the equipment during installation To prevent damage to the equipment avoid subjecting it to physical shock or violent vibrations during installation When installing the DS camera head to an optical device such as a microscope avoid causing the equipment or cables interfere with the movable unit of the optical device If you will not be using the equipment for an extended period of time unplug the power cord from the power outlet for safety Store equipment in
201. l the Java plug in If a Java plug in to run Java applets is not installed you will be requested to install a Java plug in the first time you access ROOMA Follow the displayed installation instructions e Click the image display area Image Window with Cotrol Pad Netscape a File Edit View Go Bookmarks Tools Window Help 98908 EM a amp Mal Balm 4 amp Home G Radio My Netscape CX Search EB Bookmarks Image Window with Cotrol Pad E rd Digital Sight DS L2 ROOMI Control Pad Interval Timer Quality 320240 6 640420 Click here to get the plugin 131 NETWORK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network e If the following screen appears select your Windows operating system Vista XP or 2000 in the platform box In the MIME type box select application X java applet then click Search The display of the site is subject to change If such a change occurred download and install J2SE Java Runtime Environment J2SE JRE by following the instructions on the site amp Plue in Finder Netscape a File Edi View Go Bookmarks Tools Window Help b BSS a i l Sy Plug in Finder IX AND Netscape Ma 9 A mm network Mail AIM Radio Maps Home gt Computing amp Internet gt Download gt Browser Plugins gt Plug in Finder Departments 2 What s N z 3 IL Find the Plug in You Need 3D amp Animation Th 6 aan Rasinf tion fa _ MudigVideg
202. level adjust TON1 TON7 Contrast Tone curve control A 0054 Histogram display Lb f Lig 114 52 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images Photographing procedure Follow the procedure described below for general photography By referring to the procedure below find a method that is most suitable for the purpose of observation or the intended subject as you photograph an image 1 Choose scene mode and custom mode 2 Choose display mode 3 Choose exposure mode 4 Choose photometry mode 5 Choose contrast 6 Adjust the white balance 7 Adjust the picture quality 8 Confirm exposure information 9 Save the image 10 Save photographing conditions 1 Choosing scene mode or custom mode Also for photopraphing on the Advn menu the scene mode is recommended After setting the photographing conditions according to the subject and observation method in the scene mode the detailed conditions can be adjusted easily The detailed conditions can be adjusted after selecting the custom mode and calling up the photographing conditions saved previously For selecting the scene mode or custom mode refer to 1 Selecting the scene mode or the custom mode of Section 2 1 1 Simple Photography Shortcut Menu in Chapter 6 2 Choosing display mode Display mode here refers to one that is used to determine how an image is displayed on the screen Feeling
203. lowing items are enclosed in the package Please verify that all items are enclosed If any items are missing contact your nearest Nikon representative immediately DS Camera Control Unit DS L2 DS Camera Head C mount cap HHHAMHHHITE HHH Instruction manual DS Remote Controller DS RC Remote cable E120411 optional C mount adapter optional Power cord Microscope Control License optional Control B E icense 2 VW Camera cable 3 0m E229586 ees f n A Ci ZZ m j D CUZ Chapter 2 Peripheral Equipment Peripheral Equipment 1 External display Although the DS L2 contains a 1 024 x 768 XGA 8 4 inch liquid crystal display you may need an external display if you want to observe images on a larger screen or at a higher 1 280 x 1 024 resolution SXGA Please use an appropriate PC display as described below SXGA 1280 x 1024 60 Hz non interlaced XGA 1024 x 768 60 Hz non interlaced The DS L2 is fitted with the DVI I video output terminal The DVI cable is used to connect the equipment to the digital display DVI standard 1 0 compliance The equipment can also be connected to an analog RGB display with an analog DVI cable It is recommended to use the SXGA digital display to ensure the high resolution performance NOTE If you are using an LCD display or LCD projector we recommend adjusting its clock phase and clock pitch of the display When you purc
204. mage as a Still picture CAPTURE button Saves image data A destination where image data is saved Media CF card and USB memory server via FTP or output directly to a printer can be assigned Above functions can be assigned to the button in Additional Settings of the SETUP menu 10 Chapter 4 Connecting and Installing the Equipment 1 Connection Diagrams Connecting and Installing the Equipment ENEZEM Connection Diagrams Schematic diagram Network lt J LAN cable gt q CF card PC or a PictBridge USB cable BY compatible printer L USB device mouse keyboard hub USB memory game pad L Nikon microscope DS L2 Digital DVI cable Analog DVI cable Dedicated AC adapter External display 11 Connectors DS L2 Left side Network PC or PictBridge compatible printer DS camera head External display DS AC adapter DS L2 Right side i d O Chapter 4 Connecting and Installing the Equipment 1 Connection Diagrams LAN cable qag USB cable Camera cable Digital DVI cable Analog DVI cable CF card Microdrive zx Compact Flash USB cable Ly Ly Ly HI I HI I cui EJ USB cable Remote cable E a USB mouse USB keyboard USB hub Nikon microscope DS RC USB memory USB game pad Chapter 4 Connecting and Installing the Equipment 2 Installing the DS L2 2 o Installing the DS L2 When ins
205. me rate is 20 fps This display mode is useful when good performance with moving subjects is required As two pixels are added respectively in the vertical and horizontal directions to make one pixel this mode is four times more sensitive to light than other modes making it particularly useful for dark subjects Progressive display modes eliminate color shifts even with moving subjects An image area of 800 560 pixels is enlarged for display at the monitor resolution The top and bottom 40 pixels of the 1200 vertical pixels are cropped out and therefore not displayed The frame rate is 30 fps This display mode is useful when good performance with moving subjects is required Progressive display modes eliminate color shifts even with moving subjects An image area of 1200 560 pixels corresponding to the center of the image is formatted for the monitor resolution The frame rate is 30 fps This display mode is useful when good performance with moving subjects is required Progressive display modes eliminate color shifts even with moving subjects 196 Chapter Basic Operation Method CAM E RA VARI ETY 7 Operation Differences among DS Camera Head Models Size of saved images Select the size of saved images by doing CCD source setting and setting of size of saved images on the REC menu CCD source setting represents the CCD mode during image acquisition when you freeze save or print images When S
206. memory connect the USB memory directly to the USB port of the PC USB interface boards and hubs The DS L2 supports USB 2 0 If the DS L2 and the USB interface board are connected in one of the following ways data is transferred at a rate of 12 Mbps compliant with the USB 1 1 standard USB 1 1 interface board DS L2 USB 1 1 interface board USB 1 1 hub DS L2 USB 2 0 interface board USB 1 1 hub DS L2 If the DS L2 and the USB interface board are connected in one of the following ways data is transferred at a rate of 480 Mbps compliant with the USB 2 0 standard USB 2 0 interface board DS L2 USB 2 0 interface board USB 2 0 hub DS L2 Make sure that the USB 1 1 interface board and USB 1 1 hub used in the system are compliant with the USB 1 1 standard and that the USB 2 0 interface board and USB 2 0 hub used in the system are compliant with the USB 2 0 standard To avoid unexpected problems we recommend that the interface board and the DS L2 be connected in only one hub stage and not in cascaded hub stages NOTE When multiple units of DS L2 are connected to a Windows 2000 machine equipped with a USB 1 1 interface board or a USB 1 1 hub the DS L2 units do not operate normally If connecting multiple units of DS L2 is desired use a USB 2 0 interface board and a USB 2 0 hub or use Windows XP for the OS USB cables The DS L2 does not come with a USB cable Be sure to purchase an appropriate cable that suits the USB standard of the
207. mode recommended OS Windows XP Japanese English Note Use the Windows XP Professional Service Pack 2 Other We recommend installing the Microsoft standard USB 2 0 device driver for the PC s USB controller PC monitor Item Requirement Resolution 1280 x 1024 pixels True Color mode displayable monitor recommended Nikon does not guarantee that the software will work on all the PCs that comply with the operating environments described above For detail information contact your nearest Nikon representative Chapter 2 Peripheral Equipment 12 Nikon 80i 90i Upright Microscope LV150A LV100DA LV ECON LV AFCON Industrial Microscope and AZ100M Universal Zoom Microscope If you have a Nikon 80i 90i upright microscope equipped with a digital imaging head you can purchase an optional license to control the microscope or display its microscope state on the menus of the DS L2 To connect your Nikon 80i 90i microscope to the DS L2 use the USB cable provided with the microscope If you have the LV100DA LV ECON LV AFCON industrial microscope or AZ100M Universal Zoom Microscope purchase a license to control the microscope or display its status using the menus on DS L2 To connect the LV100DA LV ECON LV AFCON AZ100M microscope and the DS L2 use the USB cable provided with the microscope For information on purchasing a license contact the nearest Nikon representative To connect the LV150A industrial microscope Nikon specifies the USB 232
208. mouse pointer on one of thumbnail images and the playback delete FTP save and print buttons will be displayed If the folder contains more than nine image files you can change pages using the lt or gt direction buttons NOTE Right click on the mouse if you want to stop a thumbnail from appearing in the middle 69 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images 2 2 4 Using Dual Window Display to Compare with the Reference Image A FREEZE Live You can split the screen into left and right halves and display a still image in the left half and a live image in the right half for image comparison You can use the electronic zoom to zoom in or out in either half of the screen If you are using a mouse you can scroll the display up or down on either screen 1 Select DWIN on the VIEW menu Approximately half of the middle part of the screen displayed before being split moves to the left to become a still or frozen image Any part of the screen that has disappeared as a result of this operation can be made to reappear by scrolling with a mouse Move the mouse pointer to the boundary and it will change shape to become a cross hair pointer Now click the mouse button and the screen Will scroll until the hidden part appears 2 To quit dual window display press SWIN on the VIEW menu NOTE Although the image can be saved while being displayed in dual windows be aware that you cannot save an imag
209. mpatible with the DS L2 7 CF card A CF card is needed to record or reproduce photographed data to or from a CF card Compatible types are TYPE I TYPE Il and Microdrive Please use a model whose operation is verified by Nikon Not every CF card is compatible with the DS L2 8 C mount lens A C mount lens is required to photograph subjects other than microscopic appearances Any lens that satisfies the following conditions may be used e Protrudes no more than 10 mm from the mount surface into the camera e he image circle of DS 2Mv DS 2MBW and DS 2MBWc is 1 1 8 The image circle of DS Fi1 DS Qi1Mc DS 5M and DS5Mc is 2 3 Use a lens for 1 or 2 3 e When using diaphragm settings brighter than F2 8 you may observe slight light falloff around the periphery 9 Network connection cable You will need a network connection cable to save image data on another server or to control the DS L2 from another computer over a network Use a cable for 10 100Base TX Category 5 or 5e To satisfy EMC standards use a shielded cable When connecting the DS L2 to a LAN use a straight cable When connecting the DS L2 directly to a PC use a crossed cable 10 USB cable You will need a USB cable to save image data to a PC or to print directly to a printer via USB It is recommended to use an AB type cable that is compatible with USB 2 0 Use a USB cable with ferrite core for stabilization of USB transfer and reduction of noise g
210. n crossline grid function Image freeze electronic zoom interval photographing two split screens CF card thumbnail display CF card reproduction 215 Recordable image Recordable image file format JPEG compression mode Interface CF card USB2 0 host interface USB2 0 device interface Direct print Network General External dimensions Weight Rated input voltage Power consumption Structure of fixed part Acoustic Noise Chapter 9 Technical Specifications 1 DS Camera Control Unit DS L2 TIFF BMP JPEG 12bit monochrome RAW only when connected to DS Qi1Mc DS 2MBW DS 2MBWc Fine Normal Basic Draft Type I Type II Microdrive USB mouse USB keyboard USB printer connectable Nikon 80i 90i upright microscopes LV150A LV100DA LV ECON LV AFCON industrial microscope AZ100M Universal Zoom Microscope Mass storage function vendor unique function compliant PictBridge compatible printer connectable PictBridge compatible printer connectable IEEE 802 3 compliant 10BASE T 100BASE TX DHCP supported HTTP server Telnet server FTP server FTP client 230 W x 200 H x 51 5 D mm not including the stand arm and protrusions 1 4 kg 12 VDC 5 70 VA when the DS camera head and DS AC adapter are attached Stand arm free stop hinge type VESA 75mm stand arm Fixable to UL1678 compatible stand and mount use the fitting holes for the stand arm Less than 70 dB Maschine
211. n Human Interface Device HID HID Mapper 37 1 4 5 Startup Condition Settings Power On Settings cccccceceeeeeeeeeeeeeeeeeeeeaees 41 1 4 6 Adjustment for Using an External Monitor cccccccccseeeeeseeeeeeeeeeeeeaeeeeesaaeees 43 1 4 7 Settings for Connecting a Microscope ccccceccceeeeeeeceeeeeeeeeeeeeeeeeeeaaeeeesaaeees 44 2 Photographing IIsges cei taptesuxsikskwa SA du a tubas rad e aaa i eau 45 2 1 Setting Camera Operations and Adjusting Images seeeeseeessss 45 2 1 1 Simple Photography Shortcut Me Nu ccccceccceseeeeeeeeeeeeeeeeeeseeeeesaeeeeesaees 45 2 1 2 Simple Photography in the DF FL Scene Mode sseeeeeeeeeeeee 49 2 1 3 Fine Adjustment before Photographing Advn Menu eeeessssees 51 2 1 4 Special Photography on Advn Menu Fluorescent Photographing or Dark Subject Photographing ccccccecseeeceseeeeeeeeeeeeeeeeeeeseeeeesaeeeesaeeeeeseneeeeas 61 3 4 CONTENTS 2 1 5 Special Photography on Advn Menu Stroboscope Photographing 63 2 1 6 Registering Items Relating to Camera Photometry REG1 Menu 64 2 2 Using a CF Card USB Memory scie ion onte inve rb dotati tanta OEE 65 22 JEGMernumtBasic FUNCION srcane o ee etu nou seu a eL 65 2 2 2 REC Menu Convenient Function ccccccccccsssceccseeeeseeesecseeeeeseeu
212. n more detail in the subsequent pages 111 NETWORK Using ROOM1 Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network ROOM allows you to observe the image that is being shot by the DS L2 at specified intervals while at the same time capturing images onto the PC or displaying a stream window You can also control the DS L2 main unit from this screen 2 3 4 8 5 1 Control Pad 6 E 7 Ci 1 Tabs Entrance Reload ROOM2 ROOMS ROOMZ 4 2 Control Pad NEL vi Quality 3207240 9 Returns you to the Entrance screen Redisplays the ROOM screen with the preview image updated Displays the ROOM2 screen Displays the ROOMS screen This tab is not displayed if an unsupported combination of operating system and browser is used Displays the ROOM4 screen This tab is not displayed if an unsupported combination of operating system and browser is used Displays a subwindow for controlling the DS L2 From this window you can save the latest image to an image file You can also perform almost all operations that you would perform from the CAM Advn menu Refer to Section 2 1 3 Fine Adjustment before Photographing Advn Menu in Chapter 6 NOTE No data or values can be entered directly from the keyboard Use a mouse to control the buttons on the WEB screen When a monochrome camera is used the settings of RH GAIN B GAIN and Sharpness cann
213. n these is being displayed set calibration values again i You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu If you perform an electronic zoom while using this function the screen display will reflect the zoom magnification If you change the optical zoom of the digital imaging head while using this function with the Nikon 80i 90i microscope connected to your system the screen display will reflect the zoom magnification Refer to the instruction manual for i Control License Card sold separately i If you change the optical zoom while using this function with the Nikon AZ100M microscope connected to your system the screen display will reflect the zoom magnification change accordingly Refer to the instruction manual for i Control License Card sold separately If you change the objective while using this function and if the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope using a motorized nosepiece or intelligent nosepiece the screen display will change accordingly Refer to the instruction manual for i Control License Card sold separately If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope using a motorized nosepiece or intelligent nosepiece note that each calibration value call number is linked to the hole numb
214. n with 1 3Mp display mode Eliminating fluctuations of exposure for moving subjects or when moving a subject while observing MANU or use AE_Lin PROG Photographing under the same conditions at all times MANU Features of exposure modes and instructions on use PROG Automatic exposure Choose PROG for general photography The DS L2 automatically determines exposure based on a combination of the shutter speed and camera sensitivity In this case the electronic shutter is automatically adjusted in the following range For display modes other than C Scan 1 s to 1 1000 s For display mode C Scan 1 15 s to 1 1000 s e If the image is excessively bright adjust the compensation icon CMP to the negative side Conversely if the image is excessively dark adjust the compensation icon CMP to the positive side e ffluctuations in exposure caused by movement of the subject presents a problem use to temporarily hold the exposure value During that time becomes red e Recommended example General photography of a relatively bright subject S AE Choose S AE when you fix the electronic shutter while photographing the subject With the electronic shutter fixed to its set value the DS L2 automatically sets the camera sensitivity e Effective in cases where the brightness of the subject changes frequently while being observed under predetermined conditions and you are concerned with the response of automatic exposu
215. nded OS Windows 2000 SP4 XP SP2 Vista Mac OS X Ver 10 3 or higher Browser Windows Internet Explorer 6 02 SP 2 7 0 Firefox 1 5 2 0 Lunascape 3 3 0 Netscape 7 1 Macintosh Safari 1 0 Firefox 1 5 2 0 102 e OS of the PC used for the FTP server and installation of an FTP server 4 Using the DS L2 via a Network NETWORK Chapter 6 Basic Operation Method If images are to be saved in an FTP server from the DS L2 you need to have a PC with FTP server functions installed Make sure that the PC used for an FTP server is running one of the supported OSs listed below Supported OS Windows 2000 SP4 Windows XP SP2 Windows Vista 4 2 2 Network settings of the DS L2 Mac OS X Ver 10 3 or higher If you are using Windows 2000 SP4 XP SP2 or Vista you must have the components for FTP server functions installed Check to see if those components are installed in your computer Setting Essential Items Network Settings In this instruction manual explanation are given assuming that the IP address of the DS L2 is 192 168 10 2 that of the server is 192 168 10 1 and that of the subnet mask is 255 255 0 0 while the gateway address is 192 168 128 1 Of course you should use the IP address assigned to you by your network administrator We must assume that network settings on the PC side LAN connection are complete Please consult your network administrator for details If you are going to co
216. networks by reducing redundant traffic Smart Browsing via caching Be eye Newsgroups C Direct connection to the Internet H Roaming Access H Composer Manual proxy configuration View Left click here Offline C Automatic proxy configuration Advanced Configuration location URD Relgad SmartUpdate Left click here 6 In the Exceptions column write the ID address of the DS L2 Refer to Section 4 2 2 Setting Essential Items Network Settings in Chapter 6 for details on the IP address of the DS L2 e Enter a period after every three digits of the IP address e If the first digits of the IP address are 00 or 0 do not enter the zeros Manual Proxy Configuration Servers Type Address of proxy server to Port ugue He Security io ETP Rie Socks Po i bo Gopher 2 ONE WAIS o r Exceptions Do not use proxy servers for domains beginning with 192 168 1 2 Use commas to separate Enter the IP address here Example When entering the IP address 192 168 1 2 7 Click OK Move the pointer T to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 109 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 4 3 2 Using WEB Screens Once a PC and the DS L2 are connected through a network it is possible to check or capture the image th
217. nformation TOOL Menu 3 3 3 Using the Count Marker to Count the Number of Points The count marker is a convenient function because it displays numbers in the order in which markers have been attached Attach a marker to each point on the image and you can count the number of points easily NOTE You need a mouse to use this function 1 Press Mark 2 Press the red blue green black white or yellow icon to determine the color of the marker 3 Click any position on the screen and markers will be displayed Numbers 1 99 are displayed on markers with counts also shown to the right of the color icons If you want to change the marker color in the middle of counts press your desired color icon That way you can count points independently for each color Or you can return to one of the colors you previously used and continue counting from the last count of that color If you failed to mark any point you can use Slo Ro cancel or redo your most recent action Up to 10 recent actions can be retrieved NOTE If you put a marker in the middle of amp l or Re recent actions from that point in time cannot be canceled or redone 4 To withdraw from the operation mode press again or press another icon You cannot erase the drawn content without pressing the 3 icon to erase the drawn content or erasing the entire overlay Es NOTE You can change the character size by selecting the Font Size check box on the OTHER ta
218. nizes the DS L2 and installs the device driver that comes standard with the OS During the installation process messages will be displayed sequentially in the task tray system tray at the lower right corner of the monitor screen indicating that new hardware has been detected You do not need to perform any specific operations dp Found New Hardware Recycle Bin lows AP Professional valuation copy Build 2505 ec Bg rite a 1 40PM USB2U Device Found New Hardware Recycle Bin lows AP Professional valuation copy Build 2505 75 go a 1 40PM USE Mass Storage Device When the Safely Remove Hardware icon is displayed in the task bar it means the PC has finished installing the driver aaj sately Remove Hardware When the DS L2 is connected to a USB 1 1 compliant USB port the message shown below will be displayed in the task tray system tray This is not an error message The DS L2 will operate as a USB 1 1 device AK HI SPEED USB Device Plugged into non HI SPEED USB Hub 4 HI SPEED USB device is plugged into a nan HI SPEED USB hub us ecvcle Bin lows AP Professional valuation copy Build 2505 fi NIKONIMG 1 bs Mg Hh ndi pm For assistance in solving this problem click this message 159 USB Chapter 6 Basic Operation Method 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 4 2 Outline of the Disk Drive When the DS L2 is connected to a PC
219. nlarminformations Verordnung 3 GPSGV 06 01 2004 Der h chste Schalldruckpegel betr gt 70dB A oder weniger gem EN ISO 7779 216 Chapter 9 Technical Specifications 2 DS Camera Head 2 o DS Camera Head DS Qi1Mc DS Fi1 Image pickup 2 3 inch square pixel 2 3 inch square pixel device 1 50 megapixel interline CCD 5 24 megapixel interline CCD Infrared N Built i Max frame rate 19 0 fps 1 3Mp 5 9 fps 5Mi i 31 5 fps 640 10 0 fps 1 3Mi 46 8 fps 320 12 0 fps 1 3Mp 24 2 fps Hi 23 3 fps C Scan Number of 1280 x 1024 2560 x 1920 recording pixels 640 x 480 1280 x 960 320 x 240 640 x 480 Quantization 12 bits Sensitivity Equivalent to ISO 800 Equivalent to ISO 64 Settings Tone 3 camera sensitivity x1 Lens mount C mount Supported lenses Protruding no more than 10 mm from the mount surface into the camera Image circle 611 mm Dimensions 77 W x 76 D x 44 H mm di up Tripod screw for cameras Body color White with logo CCD cooling Peltier cooling 1 The CCD mode is indicated in parentheses 217 Chapter 9 Technical Specifications 3 DS Remote Controller DS RC DS 2Mv DS 2MBW DS 2MBWc DS 5M DS 5Mc Image pickup 1 1 8 inch square pixel 2 3 inch square pixel device 2 11 megapixel interline CCD 5 24 megapixel interline CCD Infrared SAM dus Max frame rate 15 0 fps 2MFull 3 8 fps 5Mi 1 20 0 fps 800 6 6 8 fps 1 3Mi 30 0 fps 800 5 7 5 fps 1 3MP 30 0
220. nment 5 0 Update 9 Complete Installation Completed The Install Wizard has successfully installed J25E Runtime Environment 5 0 Update 9 Click Finish to exit the wizard e Click Restart Firefox Now and restart the browser Software Update Firefox 1 5 0 6 Ready to Install Firefox has just completed downloading an important update and must now be restarted so that the update can be installed Update Firefox 1 5 0 8 Details Click Restart Firefox Mow to close all Firefox windows and install the update Click Later Ea continue without restarting The update will be installed Ehe next time vau start Firefox Restart Firefox Now 3 Disable personal firewall blocking For details refer to Section 4 3 3 ActiveX Settings 129 NETWORK N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network For Windows and Netscape 1 Change the browser settings e Select Edit gt Preferences Netscape SEE a File Edit View Go Bookmarks Tools Window Help Undo Ctrl Z y C Redo Ctrl 128 31 30 36 ex EN E m Eras Y 7 Cut Ctrl x L E t i E 4 s ee My Netscape C Search Bookmarks Delete Del Select All Ctrl 4 Find in This Page Ctrl F Find Again Ctrl G Find Previous Ctrl Shift G Find Links As You Type Find Text As You Type Fill in Form Save Form Info Preferences e n Category select Advanced then select Enable Java
221. nnect the DS L2 to a LAN you need to enable it for network operation and set its own address To make network settings on the DS L2 select Network Set from the SETUP menu to call up the Network Settings screen shown below PowerOn Set NetWork Set File Set Addit ional zd HID Mapper Ret to Window NetWork Settings d Enable NetWork Read Only FTP WEB disable Write Control Internal Server D H C P Auto get an IP IP Address 192 168 020 002 SubNet Hask 255 255 255 000 User ID anonymous max 10 char Pass Word none max 10 char Enable FIP Client IP Authority External Server 1p address 192 168 020 001 ping test Server No ug User ID anonymous max 10 char Pass Word none max 10 char gateway 000 000 000 000 login test Save Default MAC Address 00 90 85 43 5E 39 skip 0 1 2 3 4 5 6 T 9 al Ee eE gu En EU I m OR CER T RU RS ES RR RT RR E ER LU 103 Enables disables network operation Sets the IP address of the DS L2 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network Enable Network To use a network always select this check box Read Only FTP WEB disable Write Control This is to disable writing to the internal files of the DS L2 when it is operated using a WEB browser or FTP command When this check box
222. nter the correct value again When you finished setting all press to save what you have set The server with the number you last saved is selected when the DS L2 starts up 144 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network Connection test Ping test To test the connection state of the external server execute on the Network Settings screen This function is used to inspect whether the specified server exists in the LAN This connection test is performed on the server that is shown in the IP Address column of External Server However it is necessary as a premise that the IP Address has been saved by pressing SAVE When a response is received from the server a message OK Complete is displayed Ping may not always be responded to for reasons of security functions such as packet filter firewall or security gateway Ping test may be reexecuted after disabling the security function of the server In that case restart the DS L2 first Also even when Ping was not responded to there is no problem with the server connection only if the server can be logged in in the login test described below Login test To test logging in to the server execute on the Network Settings screen A login test is performed on the server that is shown in the IP Address column of External Server by using its set User ID and Password However it is necessary as a premise that the User ID and Password have been saved
223. nts may have been set up in a way exclusive to that environment so be sure to consult the network administrator in your organization before setting up a PC This is necessary to avoid causing problems with the LAN Ask your network administrator to confirm the following points e Whether FTP can be used in the LAN only if you wish to use the FTP function e Whether image files can be transferred across the LAN e How IP addresses are managed if you wish to use a fixed IP address whether its use is permitted or if you wish to have IP addresses etc automatically acquired whether a DHCP server is available NOTE When a fixed IP address is acquired a subnet mask and a gateway address are also acquired Note however that when you apply for an IP address you may be requested to supply the MAC address of your product The MAC address is shown in the lower right corner of the Network Settings screen of the SETUP menu In automatic IP address acquisition by DHCP the assigned IP addresses change dynamically Accordingly if you are using a web browser to access the DS L2 from a PC we recommend setting a fixed IP address for the DS L2 Confirming the PC to be network connected e OS of the PC used for a Web browser and browser versions There may be slight differences in Web browser functions depending on the OS of the PC and the browser version To use a Web browser be sure to use a PC with the OS and browser recommended below Recomme
224. o redraw a reference line select the bu icon once again Press the JX icon or on the Tool menu to close the TOOL Bar NOTE T Be sure to use these functions in the state where values are labeled with the units nm um mm or cm If a unit other than these is being displayed set calibration values again z If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for i Control License Card sold separately Fi i Press the icon to hide all of the measurement results being displayed Fy Press the icon under this state to display them again You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu i You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu i Press the CLR icon to erase a measurement point existing on the image When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to erase it i Press the ROL icon to use a measurement point existing on the image for the next measurement Wh
225. of MAX ActiveX must be enabled to use ROOMS Refer to Section 4 3 3 ActiveX Settings If you have enabled ActiveX but no images display try using ROOM4 Java applets must be enabled to use ROOM4 Refer to Section 4 3 4 Java Applet Settings The DS L2 cannot be accessed from multiple browsers open on the same computer Access from browsers on several computers to a single DS L2 will cause slow down operations Normal operation is possible with up to three computers at once When one DS L2 is accessed from multiple computers the settings displayed for image quality time interval and electronic zoom may be different from the actual settings used to preview images in ROOM1 ROOMS and ROOMA This is because the most recent browser settings configured for viewing in ROOMS or ROOM4 will be applied to the preview screens of all browsers e When images are previewd on ROOM1 ROOM3 and ROOM4 simultaneously a thin white line may appear on the bottom of ROOM 1 preview window But images can be saved without any problems NOTE The screen shots shown in this section may differ depending on which browser software or version is used 110 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network Regarding the use of a browser e Depending on the PC environment and the browser version the WEB screens may behave differently or suffer some operational restrictions e When moving from ROOM1 ROOM2 ROOM3 or R
226. ographing a dark subject that requires a long exposure time use the camera head in a cool place to allow its temperature to decrease This will help to reduce star shaped noise and improve the camera performance 50 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images 2 1 3 Fine Adjustment before Photographing Advn Menu The CAM menu is displayed immediately after you display any menu or when you select the CAM tag The CAM menu provides the following functions on setting of photographing conditions e Four kinds of display modes are prepared that are classified by resolution sensitivity live speed and balance You can choose the appropriate display mode according to the subject and photographic conditions e To obtain the optimum exposure of the camera you can choose or correct photometry or exposure mode e You can adjust the color and contrast Select the Adv1 or Adv2 tab on the CAM menu and the Adv1 or Adv2 menu will be displayed In the Adv1 menu you can make various settings of the camera individually to obtain an optimum combination of settings for the intended subject In the Adv2 menu you can display histogram and adjust tone curves Configuration of the Adv1 Advanced1 menu Displayed when the cooled camera is connected CAH VIEW REC RS TOOL SETUP Lien Ls 3l s ov EDE EEE nnm Off sett ings pou 0088 Focus indicator Info box Indicates information Scene mode call bu
227. on Shared Drivers DS L2 for the Copy manufacturer s files from entry Install From Disk Insert the manufacturer s installation disk and then And then click the OK button make sure that the correct drive is selected below 2 Click the OK button 1 Specify the folder Copy manufacturer s files from C Program FilessMikon sShared DriverssD5 L2 Screen while installing Select the Nikon USB Eclipse i Microscope And then click the Next button The driver will be installed Found New Hardware Wizard Select the device driver you want to install for this hardware 1 Select the Nikon USB Eclipse i Microscope E Select the manufacturer and model of your hardware device and then click Next If you have a disk that contains the driver you want to install click Have Disk Show compatible hardware Model Nikon USB Eclipse i Microscope 2 Click the Next button A This driver is not digitally signed Tell me why driver signing is important 178 Chapter Basic Operation Method USB 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function S hen installation i leted creen when installation is complete Click the Continue button Hardware Installation AN The software you are installing for this hardware Nikon USB Eclipse 4 Microscope has not passed Windows Logo testing to verify its compatibility
228. on enables to control the DS L2 and to perform the live display or capture of the photographed image with a special application on the PC PictB The USB D connector is connected to a printer supporting PictBridge to enable direct print of photographed images Refer to Section 2 3 Outputting Images Directly to a Printer in Chapter 6 OSD Position U Left U Right Select a position on which the main menu is displayed upper left upper right Size Small Big Select the display size of the menu SXGA XGA LCD Select a monitor output state SXGA LCD OFF The internal monitor is OFF and the external monitor is ON with SXGA resolution XGA LCD OFF The internal monitor is OFF and the external monitor is ON with XGA resolution XGA LCD ON The internal monitor is ON and the external monitor is ON with XGA resolution LCD Back Light Adjust the brightness of the internal monitor DiDC I F Active The DS L2 has a plug and play function of an external monitor that complies with VESA DDC2B When this is turned ON the following functions are achieved 1 When no external monitor is connected the internal LCD is ON SXGA LCD OFF XGA LCD OFF 42 Chapter 6 Basic Operation Method SETU P 1 Preparations Before Photographing 2 When an external monitor that does not support SXGA display is connected the external monitor is ON with XGA resolution SXGA LCD OFF When you use an external monitor tha
229. onochrome camera is connected the RAW monochrome 12bit TIFF format is selectable If you choose JPEG for the file format choose the compression rate picture quality from Fine Normal Basic or Draft Choose the image size In the REC menu you can further save a comment or overlay a print along with the image Log CSV Select the Log check box and enter a comment in the input box That way you can save photographing information or comments in text format up to 60 characters at the same time you save an image You may enter the subject name number and the objective magnification for example to facilitate confirmation at a later time You may only enter alphabetical characters numbers and symbols When you save an image in BMP format the comment is saved to a file named after the image with the extension txt added When you save an image in JPEG or TIFF format it is saved to a file named after the image with the extension txt added Further it is embedded in the image file as Exif information Check the CSV checkbox to save the measurement results on the screen as a CSV format file at the time of saving the image Overlay print Overlay data such as pen drawing text scale and calculation results are saved overlapping on top of the image in the same position as displayed on the screen When you put check marks on respective items in Paste to Image on the OTHER tab on the Tool
230. os pa ses Europeus Este s mbolo indica que este produto para ser recolhido separada mente Esta norma aplica se s para os utilizadores nos pa ses Europeus e Este produto est designado para recolha de res duos em separado num recipiente apropriado N o deitar no caixote do lixo dom stico e Para mais informac es contactar o revendedor ou as autoridades locais respons veis pela gestao dos residuos Simbolo per la raccolta differenziata applicabile nei paesi europei Questo simbolo indica che il prodotto va smaltito separatamente La normativa che segue si applica soltanto agli utenti dei paesi europei e prodotto designato per lo smaltimento separato negli appositi punti di raccolta Non gettare insieme ai rifiuti domestici e Per maggiori informazioni consultare il rivenditore o gli enti locali incaricati della gestione dei rifiuti This symbol is provided for use in the People s Republic of China for environmental protection in the fields of electronic information products c0 x 74l amp 4 EO 3 TRIZIRIT 7 500 C B Ht Saw RE TEBJELCU ES THERES 5323 EPIC z G m Symbol for kildesortering i europeiske land Dette symbolet indikerer at produktet skal kildesorteres De nedenst ende punktene gjelder for alle europeiske brukere e Dette produktet skal kildesorteres og innleveres til dedikerte innsam lingspunkter Ma ikke kastes med normalt husholdningsavfall e For mer informasjon ta kontakt med
231. ot be specified on the subwindow Note that those settings are still displayed on the subwindow but inoperative 112 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 4 Control Pad Microsoft Inter m X Enable Control Enable Control Program Sets or adjusts the DS L2 i Be sure to deselect the check box labeled Read Only FTP 5 WEB disable Write Control in Network Settings of the SETUP Em wel menu of the DS L2 before making any settings here White Balance TONE Effect Y_GAIN R_GAIN B_GAIN E Ce Download BMP Download BMP mai 640 480 J 1280 960 2560 1920 Downloads an image file in BMP format Download TIFF 640480 12 0 960 2560 1920 Download TIFF Pick up JPEG Downloads an image file in TIFF format 640 480 1280 960 2560 1920 Jpeg Quality Normal n Pick up JPEG Gets an image file in JPEG format To close this control pad click the button at the upper right corner This control pad is automatically closed when you go to another screen Entrance ROOM2 ROOMS or ROOM4 e Download BMP Download TIFF Click the image size you want to download and the latest image in that size will be downloaded into the PC in BMP or TIFF format 1 When you click any image size to select Save it to disk a screen is displayed for you to select the folder in the PC that you wish to sa
232. ource Full is selected the CCD mode is automatically switched to SMi CCD Mode 3 during image acquisition and images with high resolution can be obtained Note that switching CCD modes will result in a brief delay When Source Now is selected images are acquired in the current CCD display mode This setting in which the CCD mode is not switched is recommended when you expect to freeze images repeatedly CCD source setting Source Now Setting of size of saved images 1600 100 When the CCD source setting is Source Now and the CCD mode is 2MFull select 1600 1200 800 600 or 400 300 for the setting of size of saved images When the CCD source setting is Source Now and the CCD mode is 800 6 select 800 600 or 400 300 for the setting of size of saved images When the CCD source setting is Source Now and the CCD mode is 800 5 select 800 560 or 400 280 for the setting of size of saved images When the CCD source setting is Source Now and the CCD mode is C Scan the setting of size of saved images is fixed to 1600 560 When the CCD source setting is Source Full the CCD mode is automatically switched during image acquisition regardless of the current CCD mode Thus you can select 1600 1200 800 600 or 400 300 for the setting of size of saved images The following table gives the resulting file sizes for various image file
233. out any changes Only live images can be saved e Images in the RAW format saved in the DS L2 cannot be opened by some browsers for PCs e Images in the RAW format saved in the DS L2 are darker than images in other format 198 Chapter 6 Basic Operation Method CAM E RA VARI ETY 7 Operation Differences among DS Camera Head Models Magnification for Observation and Printing CCD pixel size of DS camera heads The below list shows the pixel size maximum number of recording pixels and effective area actual dimensions of the recording pixel area of each DS camera head T size um No of recording pixels Effective area mm Camera head DS 2Mv DS 2MBW 1600 1200 DS 2MBWc Calculating the magnification at the time of observation on the monitor Use the following formula to calculate the magnification of a subject on the display monitor Magnification on the monitor Magnification of the objective x Magnification of the relay lens x Diagonal size on the monitor Diagonal size on the CCD effective area lt Calculation examples gt e g 1 Magnification on the monitor 40 x 0 7 x 213 10 88 approx 550 times where the camera head is DS Fi1 DS 5M or DS 5Mc the magnification of the objective is 40 times that of the relay lens is 0 7 times and the monitor inch size is 8 4 diagonal size 213 mm Magnification on the monitor 40 x 0 55 x 213 8 8 approx 530 times where the camera head is DS 2Mv DS
234. ow Voltage Directive This product conforms to the Korean MIC Mark approval 220 En Symbol for separate collection applicable in European countries This symbol indicates that this product is to be collected separately The following apply only to users in European countries e This product is designated for separate collection at an appropriate collection point Do not dispose of as household waste e For more information contact the retailer or the local authorities in charge of waste management Del Symbol f r getrennte Wertstoff Schadstoffsammlung in europ ischen L ndern Dieses Symbol zeigt an dass dieses Produkt separat entsorgt werden muss Folgendes gilt f r Verbraucher in europ ischen L ndern e Dieses Produkt darf nur separat an einer geeigneten Sammelstelle entsorgt werden Eine Entsorgung im Hausm ll ist unzul ssig e Wenden Sie sich f r n here Informationen bitte an Ihren Handler oder die rtlich f r Abfallentsorgung zust ndigen Beh rden pai Fri Symbole pour la collecte s lective applicable aux pays europ ens Ce symbole indique que ce produit doit tre collect s par ment Les mesures suivantes concernent uniquement les utilisateurs euro p ens e Ce produit doit tre jet s par ment dans un point de collecte appro pri Ne jetez pas ce produit dans une poubelle r serv e aux ordures m nag res e Pour plus d information contactez le d taillant ou les autorit s locales res
235. pace Deletes one character during character entry SHIFT Backspace Deletes the entire text during character entry NOTE The DS L2 is factory set to a Japanese keyboard layout If you wish to use an English keyboard issue the Telnet command shown below to change keyboard settings To revert to the Japanese keyboard use the Telnet command shown below to change keyboard settings w_KYBUS Selects an English keyboard w_KYBJP Selects a Japanese keyboard Settings you have changed will take effect upon restarting the DS L2 after executing the command For information on Telnet connections refer to Section 4 5 Using Telnet Commands to Operate on the DS L2 in Chapter 6 ED SETUP Chapter 6 Basic Operation Method 1 Preparations Before Photographing 1 1 5 USB Barcode Reader You can use a USB barcode reader instead of the USB keyboard When you use the USB barcode reader characters read out from the barcode are entered instead of your entering characters through the keyboard Be sure to use a USB barcode reader whose operation is verified Not all USB barcode readers are compatible with the DS L2 NOTE In the DS L2 a special mode is available when a barcode reader is used The operations of reading the barcode characters with the barcode reader and saving the image to file using a file name made up of the characters read with the barcode reader are executed together The save destination is determined in accordance with the
236. peration Method N ETWORK 4 Using the DS L2 via a Network Using FTP commands to operate the DS L2 from a PC e Get images from the DS L2 e Send and save images to the DS L2 e Control the DS L2 in an advanced way cet m FTP commands may be used from a PC to receive send or save image files Furthermore advanced control of the DS L2 is possible using the DS L2 s FTP server function Using Telnet commands to operate the DS L2 from a PC e Control the DS L2 in an advanced way ein gt Telnet commands may be used from a PC to achieve advanced control of the DS L2 In this case the DS L2 s Telnet server function is used Operating on a PC from the DS L2 e Send images to an FTP server ul gt Em Images can be transferred to a specified FTP server NOTE This function cannot be used unless an FTP server facility is available on the PC side Because up to five FTP servers can be specified images can be easily and quickly filed in a personal directory created separately one for each login user Furthermore when this function is combined with image file base software a more efficient image filing system may be created In this case the DS L2 s FTP client function is used 101 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 42 o Network Settings 4 2 1 Items Needing Confirmation Items Needing Confirmation A LAN in some DS L2 operating environme
237. ponsables de la gestion des ordures Izd 5 S mbolo para recogida separada aplicable en pa ses Europeos Este s mbolo indica que este producto se recoger por separado Lo siguiente s lo se aplicar en pa ses Europeos e Este producto ha sido designado para su recogida en un punto de al macenamiento apropiado No lo tire como un deshecho dom stico e Para m s informaci n contacte con el vendedor o autoridades locales al cargo de la gesti n de residuos Dk Symbol for special bortskaffelse af denne type produkter i de europ iske lande Dette symbol angiver at dette produkt skal bortskaffes specielt Det efterf lgende er kun til forbrugere i de europ iske lande e Dette produkt skal bortskaffes pa fx en genbrugsplads el lign Det ma ikke smides vaek som normalt husholdningsaffald e For yderligere information kontakt din forhandler eller de lokale myndigheder som fx teknisk forvaltning INI Symbool voor gescheiden inzameling zoals dat wordt gebruikt in Europese landen Dit symbool betekent dat dit product apart moet worden ingezameld Het volgende is alleen van toepassing op gebruikers in Europa e Dit product dient gescheiden ingezameld te worden op een daartoe bestemd inzamelpunt Niet wegwerpen bij het normale huisvuil e Neem voor meer informatie contact op met het verkooppunt of met de lokale instantie die verantwoordelijk is voor het verwerken van afval S mbolo para recolha de res duos em separado utilizado n
238. pparent focus Although reducing the aperture diaphragm extends the depth of focus doing so will also reduce resolution Take this into consideration and adjust the aperture diaphragm in accordance with your needs 4 Adjusting the Focus Adjust the focus of the microscope so that the image can be clearly seen on the display 5 Adjusting the Lamp Voltage When photographing a color image the color reproducibility of the image depends on the lamp voltage When a halogen lamp is used as the light source increasing the lamp voltage produces a bluish light while decreasing the lamp voltage produces a reddish light Except in cases where it is specifically necessary to adjust the tone the voltage should be set to the proper level for microphotography which depends on the microscope being used Since this level varies for each type of microscope refer to your microscope s instruction manual If the lamp voltage changes measure the white balance once again 20 Chapter 5 Microscope Adjustment 6 Adjusting the Exposure Time Use an ND filter to adjust the illumination for the microscope to a level that yields a suitable exposure time generally 1 15 to 1 250 s You can also adjust the camera sensitivity Terms What is an ND filter An ND filter is a filter that affects only the amount of light passed not the color balance of the light For example an ND2 filter cuts transmitted light in half while an ND16 filter reduces
239. profile of a user defined cross section can be displayed in the Profile image For details on the operation method refer to the online help on the application NIS Elements Freeware does not include functions for controlling Nikon 80i or 90i series upright microscopes For this reason do not use the following setup PC USB Cable DS L2 USB Cable E gt 80i 90i Upright NIS Elements USB H USB D interface interface Freeware Microscope NIS Elements Freeware cannot be used while the i Series Support Tools control and configuration software is running 2 For the NIS Elements software sold separately or other applications dedicated to the DS L2 Operate the DS L2 with reference to the instruction manual provided with each software Keep the following requirements in mind when running the NIS Elements software This is because if the computer enters the Standby mode live images will not be updated after the computer wakes up Other problems may also arise Do not close the computer if it is a notebook Do not press the Sleep button on the computer Do not put the computer in Standby mode manually from an operating system menu 183 CAM E RA VARI ETY Chapter 6 Basic Operation Method 7 Operation Differences among DS Camera Head Models O7 Operation Differences among DS Camera Head Models Seven DS camera head models are available as described in Section 1 in Chapter
240. r User ID and Password input Set the DS L2 as the login user 3 Specify the home directory Specify the home directory when logged in 4 Enable writing to the server Enable writing of image files to the FTP server 189 NETWORK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network Adding components If Internet Information Service IIS does not have components added you need to install them For Windows 2000 Professional and XP Professional 1 2 3 Open Control Panel and select Add Remove Programs Add Remove Windows Components Select Internet Information Service IIS and click the Advanced button Examine whether the FTP File Transfer Protocol Services check box is selected If not select it and click OK After that follow the installation instructions You will be requested for a Windows 2000 or XP CD ROM during this course If you are using a preinstalled version it should normally be copied to a location below the System directory on drive C Thus you only need to specify the directory that contains the required files in order to have them installed If the files cannot be found other folders will be searched For Windows Vista 1 2 Settings Open Control Panel and select Programs and Features Turns Windows features On or Off Turn on the Internet Information Services check boxes FTP server and FTP management console listed under FTP publish s
241. r On Settings on the SETUP menu and press SAVE NOTE Even if you have set the DS L2 to display grid lines at power on the grid lines will be hidden if you choose to hide it or use a superimpose function If this happens redisplay grid lines by using the menu 94 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu 3 5 3 Using a Scale to Measure the Subject Size ium You can display a scale on the screen and use it to measure an approximate size of the subject 1 Select the calibration value you want to use from m1 through m7 2 Press Scale and a scale matched to the selected calibration value will be displayed 3 Press Scale again to erase the displayed scale Normally a scale is displayed on the screen only and is not drawn on the saved or printed image If you select the Scale check box on the OTHER tab two scales one vertical and one horizontal will be drawn in the lower right part of the saved or printed image NOTE i Depending on the calibration value such as when the screen full length is calibrated as being equal to 1 mm the system may not be able to display values In such a case the value is displayed as ZEHHHHE and you must change the unit of the calibration value from 1 mm to 1000 um etc 2 Be sure to use these functions in the state where values are labeled with the units nm um mm or cm If a unit other tha
242. r folder name and the folder at the level directly below it and the JPEG format files and BMP format files in that folder all are displayed 116 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 5 Files in the CF card or USB memory Shows the file names created date time and log comments of the JPEG format files BMP format files and TIFF format files The log comments displayed here are the contents of the log text that you attached from the REC menu when saving images e Method for downloading files 1 Choose any image file by clicking on its icon or file name and a screen will be displayed for you to select the folder in the PC to which to save 2 Choose the folder in the PC where you want to save the selected image file Change the file name before saving as necessary NOTE If you have difficulty in opening an image file in the CF card or USB memory that is inserted in the DS L2 directly from a PC on a network temporarily save the file to the PC first and then open it on the PC 6 Allocation of the CF card and USB memories to drives Select one from a CF card and USB memories to display its list of image files The CF card is allocated to Drive C and the USB memories are allocated to Drives D to F The USB memories are allocated to Drives D to F sequentially according to the inserting order Using ROOM3 ROOMS displays the image currently being shot with the DS L2 at a specified inter
243. r immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Click the Continue button Continue Anyway STOP Installation Files Needed Specify C Program Files Nikon Shared Drivers DS L2 for the Copy From box The file DsCamUsb sys on Unknown is needed Cu And then click the OK button Cancel Type the path where the file is located and then click OK 2 Click the OK button 1 Specify the folder Copy files from Click the Finish button Completing the Found New Hardware Wizard The wizard has finished installing the software for 339 Nikon USB DS Camera Click here Click Finish to close the wizard 1 6 Chapter Basic Operation Method USB 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function 3 Install the ECLIPSE i series driver Next drivers for ECLIPSE i series is installed automatically a The PC automatically searches for new hardware J Found New Hardware Mikon USB Eclipse i Microscope Click the Install the hardware that manually select Walcome to ihe Found New from a list Advanced And then click Next Hardware Wizard This wizard helps you install software for Nikon USB Eclipse i Microscope If your hardware came with an installation CD 1 Click here lt 6 or floppy disk insert it now
244. r of the circle 3 Click at three points for circle measurement and a circle tracing the three points is displayed The diameter and circumference will be displayed in the vicinity of the center To redraw a circle use or to cancel or redo your most recent action 4 Press the X icon or on the Tool menu to close the TOOL Bar NOTE Be sure to use these functions in the state where values are labeled with the units nm um mm or cm If a unit other than these is being displayed set calibration values again If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for Control License Card sold separately Fy Press the icon to hide all of the measurement results being displayed Fi Press the icon under this state to display them again You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu Press the CLR icon to erase a measurement point existing on the im
245. r of the external input device must be TTL compatible e The ground potential of the external input device must match that of the DS L2 3 3V 4 7 KQ 100 Q O E e Pins 1 and 2 2 s Level whenon OVto0 8V Level when off 2 0Vto 3 3V L GND ye DV Pin 3 ON DS L2 External input device GND ZN CAUTION When plugging a stroboscope or other external device into the EXT I O connector of the DS L2 make sure the external device is off If the external device is left on while connected the resulting light emission may damage your eyes 8 Connecting to a Power Supply To prevent electric shock turn off the power for the DS L2 before making any connections Complete all other connections before plugging into a power supply When connecting the DS L2 to a power supply always use the specified AC adapter DS AC adapter For AC adapter specifications see Section 4 DS AC Adapter in Chapter 9 Technical Specifications Connect the dedicated power cord to the DS AC adapter Using a wrong AC adapter or power cord may lead to a fire or other dangerous conditions This product belongs to electric shock protection class and must be connected to a power outlet with a protective ground terminal When the power cord is lost or damaged contact your nearest Nikon representative 18 Chapter 5 Microscope Adjustment Microscope Adjustment When the camera head is mounted on a microscope adjust the microscope as de
246. rOn Settings 3 PowerOn Settings No 1 CAM Custom No B 0 turn off Short Cut Menu m small USB Device Show tools a Cross a Grid Ea Mass 8 E Vendor u PictB Cam OSD HCM Bio B etc Language English J a4 OSD Position U Left Size Big XGA SXGA XBA LCD E P EX oc I F Active 1 LCD Back Light JE 5 Display YGA Color Adjust Mirror Normal Default DS L2 Ver 320m 0601 0298 070720 If the menu language is not set to English 1 Select English in the Language field 2 Press the Save button to save the settings 3 Press the Ret to Window button to close the PowerOn Settings screen 23435 S ETU P Chapter 6 Basic Operation Method 1 Preparations Before Photographing Display a menu and select the SETUP tag to enter the SETUP menu Select Additional from the SETUP menu to display the Additional Settings screen shown below In the Additional Settings screen set the items indicated below Sets date and time e Bis Sets power save timer Sets mouse speed Sets electronic zoom magnification Assigns CAPTURE button function Sets external I O port Sets direct print Eos 1 2 3 4 5 9 When you have finished setting each item press SAVE to save the data that has been set Or press Default to restore data to default values 34 Chapter 6 Basic Operation Method SETU P 1 Preparations Before Photographing Date Time Enter a date and
247. re e o photograph a moving subject while stopping it with an electronic shutter use this exposure mode in combination with 1 3Mp 2Mfull or 800 5 display mode e ffluctuations in exposure caused by movement of the subject presents a problem use to temporarily hold the exposure value During that time becomes red e Recommended example Stereoscopic microscope observation MANU Choose MANU if you are always photographing under the same exposure conditions or photographing special subjects In this mode the electronic shutter speed or the camera sensitivity is manually set in the Advn menu e he electronic shutter speed can be set from 1 1 000 s to 60 s for the non cooled camera and 1 1 000 to 600 s for the cooled camera e The camera sensitivity can be set from x1 C gn 100 to x46 C gn 4600 54 Chapter 6 Basic Operation Method CAM E RA 2 Photographing Images e Effective for fluorescent photography where careful consideration is required to determine the exposure as well as for subjects that have partial highlight reflections e Recommended example Fluorescent photography stereoscopic microscope observation metal photomicrography F AE Choose F AE when the subject is dark image updates go slow and you have difficulty in adjusting the focus Increase the camera gain to prevent the exposure time from increasing e n this exposure mode bright dot shaped or strip shaped noi
248. rection added e The background is mostly black and the subject is sporadically bright Peak hold photometry 55 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images Selecting a photometry area e 8 fol Lt Lo Lon Use one of the photometry area icons to specify a range of the screen in which you want the exposure to be adjusted Normally a wide photometry range is recommended To avoid influences from the periphery of the screen choose a narrow photometry range You can set the size and the position of the area freely on a1 to a3 NOTE Displaying and setting photometry area The photometry range is displayed with a red frame for a limited time when the size of the photometry area is changed The red frame is kept displayed when the icon is right clicked with the mouse When the icon is right clicked again the I3 photometry area button is displayed with a frame and you will have a chance to set the AE area Operate the mouse to set the area By dragging the frame of the photometry area you can change and set the size of the photometry area By dragging the center of the photometry area you can change and set the position of the photometry area Note that the red frame disappears and the settings are cleared if the icon is right clicked once more For registration of the set photometry area refer to Section 2 1 6 Registering Items Relating to Camera Photometry REG1 Menu in Chapter 6 5 Choosing
249. reen and magenta red and cyan and blue and yellow are all pairs of complementary colors When complementary colors overlap their respective hues cancel each other out 19 Chapter 5 Microscope Adjustment 2 Adjusting the Field Diaphragm Adjust the field diaphragm so that it circumscribes the viewfield The field diaphragm has a significant impact on contrast especially for fluorescent specimens against dark backgrounds Make sure you adjust the field diaphragm correctly For details on adjustment refer to your microscope s instruction manual 3 Adjusting the Aperture Diaphragm When using diascopic illumination Generally the numeric value of the condenser aperture diaphragm should be adjusted to approximately 70 to 80 of the numerical aperture N A of the objective When using episcopic illumination Generally the aperture diaphragm should be adjusted to approximately 70 to 80 of the size of the pupil of the objective that can be seen by removing the eyepiece and looking into the eyepiece tube Supplement For details on adjusting the aperture diaphragm refer to your microscope s instruction manual For photographed images that have greater depth of focus close down the aperture diaphragm Note that closing down the aperture may limit the performance of the objective Terms What is depth of focus Depth of focus refers to the range along the axis of the field of view over which the image is in a
250. rest is low lighted adjust the exposure so that the distribution will be shifted toward the right Knowing the exact level of exposure that is needed while you adjust the exposure will help to prevent insufficient gradation during image processing 9 Saving the image Use Capt button or the CAPTURE button on the DS L2 to save the image Capt button and the CAPTURE button on the DS L2 are functionally the same The image is saved to the destination that has been assigned to the button NOTE The CAPTURE button can be assigned any desired function by the Capture Function on Additional Settings whether it be saving to a medium saving to an FTP server or outputting directly to a printer Refer to Section 1 4 2 Date and Power save Settings Additional Settings in Chapter 6 for details You can save or print images from a shortcut menu or the REC menu Refer to Section 2 2 1 REC Menu Basic Function in Chapter 6 or Section 4 6 3 Saving Image Files in the FTP Server in Chapter 6 for details on how to use the REC menu 10 Saving photographing conditions In the Advn menu you can save the set values of individual subjects and objectives in each custom mode 1 From the Advn menu set conditions such as camera operation mode and exposure NOTE Press to temporarily clear the set values revert to the initial values set when shipped from the factory 2 Select the REG tab to enter the Registration menu 3 Select
251. rom the DS L2 7 Microscope Control from the DS L2 Symptom Check points Unable to control e The DS L2 is of limited application for the Nikon 80i 90i LV150A microscope functions LV100DA LV ECON and LV AFCON This function cannot be used Or incorrect control with a microscope from a different company or other Nikon microscope results models e Turn on the power switch for the microscope e Wait until the microscope is ready for operation It takes about 30 seconds for the microscope to be ready Connect the USB cable securely between the DS L2 and the digital imaging head on the Nikon 80i 90i LV150A LV100DA LV E CON LV AFCON AZ100M microscope e Use the USB cable supplied with the microscope to connect the 80i 90i microscope or LV100DA LV ECON LV AFCON to the DS L2 Check that the DS L2 is connected securely to the LV100DA LV ECON LV AFCON AZ100M microscope using a USB cable Connect the DS L2 and the LV150A microscope with the USB RS232C conversion cable specified by Nikon Check that no hub is cascated to the hub if used between the interface board ant the DS L2 Nikon recommends using one hub or none Check whether the microscope indicates an abnormal state by buzzer or LED display In the event of any abnormality on the microscope consult the instruction manual of the microscope Unable to display e Make sure the microscope initial setting has been performed microscope status Or
252. rt media is displayed when you click the icon for the media drive A similar message is displayed when you click the icon for the CCD image drive When you connect the DS L2 other USB devices already connected to the PC will immediately become unusable In such a case change the drive letter assigned to the media drive or the CCD image drive The following shows how to make this change Select Start Control Panel Administrative Tools Computer Management Disk Management Right click the volume whose drive letter you want to change and select Change the drive letter and path Click Edit and choose one of unassigned drive letters from the select box or enter a drive letter directly from the keyboard and click OK Click OK in the ensuing confirmation dialog box 160 Chapter Basic Operation Method USB 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function 5 4 3 Using the Media Drive Receiving image files Insert media into the card slot of the DS L2 and display the Explorer screen from the confirmation screen described above You can read out the media Or you can double click the media drive removable disk icon located below Devices with Removable Storage on the My Computer screen to read out the media The image files on the media drive can be retrieved from the media in the same way as files are normally manipulated on a PC NOTE Under the default cond
253. s More options Install Don t Install While Files From the Internet can be useful this File type can potentially harm vour computer Only install software From publishers you trust what s the risk Java TM Installer Em Ed Java TM Technology Sun Microsystems Inc Java TM technology powers the best Interactive content on the web 123 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network e Select I accept the terms in the license agreement and click Next ie Java 2 Runtime Environment 5E v1 4 7 11 License License Agreement Please read the Following license agreement carefully Sun Microsystems Inc Binary Code License Agreement for the JAVA 2 RUNTIME ENVIRONMENT J2RE oe LTANDARD EDITION VERSION 1 4 2 X SUN MICROSYSTEMS INC CSUN IS WILLING TO LICENSE THE SOFTWARE IDENTTETED REDCAR TO YOU ONLY UPON CO Ida not accept the terms in the license agreement e Select Typical and click Next ie Java 2 Runtime Environment SE v1 4 2 11 Setup Type Setup Type Choose the setup type that best suits your needs Please select a setup type All recommended Features will be installed Specify the installation directory and choose which program features to install You can change your choice of features after installation by using the Add Remove Programs utility in the Control Panel Recommended Far advanced users 124 Chapter Basic Operation Method N
254. s so that files will be named after the photographed date and time 5 6 Press to set the FTP server as the destination Press to change to Continue The Cnt and Int icons will be displayed In Cnt set the number of frames to be photographed Up to 500 frames can be set If you select the Endless option images are photographed continuously until you press the Stop or until the destination medium CF USB memory or FTP is full In Int set a photography interval time It can be set anywhere from 10 seconds to 6 hours Choose the desired interval time from the following 10s 15s 20s 30s 45s 60s 1m30s 2m 3m 4m 5m 6m 10m 15m 20m 30m 45m 60m 1h30m 2h 3h 4h 5h 6h Press FTP and interval photography will start with images saved to the FTP server Press to stop photography in the middle Depending on the length of time required for network transfers the DS L2 may not be able to save images at specified time intervals Prior confirmation will be necessary before you use interval photography If images cannot be saved at specified intervals During normal operation V Photographing V Photographing V Photographing Int set value Int set value Int set value Save Save Save When set intervals cannot be maintained V Photographing V Photographing Int set value Int set value Int set value Sa
255. s powered on However to prevent data from being corrupted by removing the DS L2 during write operation follow the procedure described below 1 Eject the media from the media drive before removing the DS L2 2 Unplug the USB cable or turn off the power to the DS L2 3 When the below is displayed click OK z Unsafe Removal of Device 2 xl fou have unplugged ar ejected a device without stopping it LInplugaing ar ejecting devices without first stopping them can often cause your computer to crash and lose valuable data To safely unplug or eject any of the following devices first use the Hardware wizard in the Control Panel to stop the device USE Mass Storage Device EJ Generic volume IF vau frequently need to unplug this device Windows can give you an icon on the taskbar to quickly unplug or eject your device IF you would like to use this option check the following v Show Unplug Eject icon on the taskbar Plug in the USB cable and turn on the DS L2 The device will be recognized by the PC again It is recommended to set the date and time information within the file settings such that file names are automatically generated 158 Chapter 6 Basic Operation Method USB 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function DFTE Using the DS L2 in Windows XP 5 4 1 Installing the Device Driver When the DS L2 is connected to a PC for the first time the PC automatically recog
256. s the icon under this state to display them again You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu i Press the CLR icon to erase a measurement point existing on the image When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to erase it i Press the ROL icon to use a measurement point existing on the image for the next measurement When the cursor is moved in the vicinity of the measurement point a frame appears Left click on it to select it Press the cu icon to save a measurement result in CSV format The destination depends on the settings on the REC menu Press the aX icon to select and delete a measurement result pa Press the X 3j icon to update information being displayed 85 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu 3 4 6 Measuring Circles You can measure the diameter and circumference of circles drawn on the screen and save circles and measurement results diameter and circumference as part of the overlay for the image 1 Press T Bar The tool bar shown below will be displayed Select the 0 icon NOTE Check if the icon is selected 2 Press a color icon red blue green black white or yellow to set the colo
257. scribed below 1 Use the Appropriate Filters When capturing a color image Insert the NCB filter into the optical path When capturing a monochrome image Insert the appropriate filter into the optical path depending on the photographic purpose Generally inserting a green interference filter GIF will heighten contrast Contrast is enhanced by inserting a filter with a color complementary to the specimen Supplement The method for inserting and removing a filter varies from microscope to microscope Refer to your microscope s instruction manual A third party color compensation filter CC filter can be inserted into the illumination optical path of the microscope to compensate for changes in color balance caused by the length of the exposure time When using a phase contrast microscope or an interference microscope two luminous fluxes or multiple luminous fluxes you can enhance contrast using a green interference filter GIF or a monochrome interference filter IF We recommend simultaneous use of an NCB filter and a green interference filter GIF for the DS Qi1Mc DS 2MBW and DS 2MBWc Some microscopes may require a heat wave absorption filter Terms What is an NCB filter An NCB neutral color balance filter is a color balancing compensation filter used to adjust color temperature to daylight values in microscopes that use a halogen bulb as a light source What is a complementary color For example g
258. se will stand out on the displayed image because the gain is high but when using exposure start button the image is photographed after automatically increasing the exposure time and decreasing the gain e Even when F AE mode is selected if the subject is relatively bright the original combination of display mode and source will be followed e When using Il freeze button the noisy image will be paused NOTE Adjusting the focus of a dark subject can also be performed by pressing the focus button When the button is right clicked the camera gain is increased to adjust the exposure automatically so that the exposure time will not be too long Then the live image is displayed while keeping the exposure status When right clicking on the P focus button to increase the response at focusing NR OFF status is set Left click on the Kp button to access manual mode without adjusting the exposure 4 Choosing photometry mode There are two photometry modes m Average photometry um Peak hold photometry Normally average photometry should be selected The icon indicates the currently selected photometry mode Sample settings that suit different uses are described below Try these settings before determining your own settings e There are few luminance changes over the entire screen that has a bright field Average photometry e Whites on the background need to be whiter Peak hold photometry with exposure cor
259. serial numbers recycle and start from 1 again Therefore it is possible that one of the existing files will be overwritten For folder names too selection of Auto for FTP Dir will help avoid overwriting files as a new folder is created for each date NOTE If the DS L2 is left unattended for a long time without being connected to a dedicated AC adapter the timer may be delayed or stop Please remember that the timer will be delayed or stop after about four days have passed If you have left the dedicated AC adapter disconnected for several days reset the clock 146 Chapter Basic Operation Method N ETWORK 4 Using the DS L2 via a Network 4 6 3 2 Using the CAM Menu to Save Image Files Select the REC tag at the top of the CAM menu screen to display the REC menu In the REC menu you can specify a folder and a file name before saving an image You can also choose interval photography which lets you shoot an image repeatedly at given intervals The following explains how to save images from the REC menu Configuration of the REC menu VIEW REC NEA Press to save an image to a CF card Press Or to save an image to a USB memory E D E F z Press to save an image to an FTP server e save Press to save an image to a specified destination Dir Specify a folder name File Specify a file name Cont inue Ey Tshot 1shot Only one image is saved Continue Interval photography photographed at given
260. settings Since actual file sizes of JPEG images can vary widely from subject to subject the JPEG values given here are intended to serve only as a guide eee Fine Normal Basic Draft The size of saved images can be set in the same manner through the operation on the WEB screen operation by the FTP command operation by the Telnet command and operation with USB connection 197 CAM ERA VARI ETY Chapter 6 Basic Operation Method 7 Operation Differences among DS Camera Head Models Save in the RAW format For the DS 2MBW and DS 2MBWc the RAW format is added to the file type table shown above In the RAW format 12bit monochrome data is saved as TIFF format image Saving in the RAW format is recommended for edit of images using a PC Additionally the image file capacity can be reduced to 2 3 of that in the TIFF format by saving in the RAW format Refer to the following table for image file capacity File Type RAW TIFF 1600 x 1200 3 6MB 1600 x 560 1 6MB 800 x 600 900KB 800 x 560 800KB However check the following items when saving data in the RAW format e When the CCD source is Source Now on the REC menu the data can be saved in only the size displayed in the CCD display mode When the CCD source is Source Full the data can be saved in 1600 x 1200 size e Inthe RAW format the data displayed in two screens or the data edited on the Tool menu cannot be saved because the data from the camera is saved with
261. spective companies The TM and marks are not used to identify registered trademarks and trademarks in manual The character font data used in this equipment is provided by Morisawa amp Company Ltd and the related copyright belongs to Morisawa amp Company Ltd N WARNING 1 Using the equipment This equipment must be used for photographic purposes and image display purposes only Do not attempt to use it for any other purpose 2 Do not disassemble or repair the equipment The DS L2 and DS AC adapter contain high voltage power supplies Never attempt to disassemble or repair the equipment by yourself doing so may result in electric shock or equipment failure Never attempt to disassemble any part of the equipment unless instructed to do so in this manual If you notice any problems with the equipment turn off the power and contact your nearest Nikon representative 3 DS AC adapter Be sure to use a dedicated AC adapter Use of any other AC adapter may result in fire or electric shock For AC adapter specifications see Section 4 DS AC Adapter in Chapter 9 Specifications 4 Power cord To prevent electric shock always turn off the POWER switch on the DS L2 when plugging in or unplugging the power cord For the DS L2 use only the dedicated AC adapter and the specified power cord The specifications for the power cord are given below Using another power cord may lead to fire or other hazardous conditions Because this
262. sposal to a professional vendor as special industrial waste or dispose of it according to the regulations or rules of the municipality ZN CAUTION 1 Installation environment Do not use this equipment in locations subject to high temperatures high humidity vibration or excessive amounts of dust Doing so may result in fire or malfunction Because the DS cooled camera is equipped with a cooling mechanism it is slightly more susceptible to condensation than typical electronic devices if used for extended periods in locations subject to sudden temperature changes from cold to hot or vice versa or high humidity Pay particular attention to the ambient humidity when using the DS cooled camera for extended periods After extended use be sure to leave the camera off for a few hours before turning it on again When used for an extended period of time the DS L2 may become hot Do not place the DS L2 on a surface that cannot withstand heat such as vinyl or plastic Always install the equipment with a surrounding clear area of 100 mm or more The DS L2 can be set to a commercially available arm of VESA 75 mm standard compliance or a stand or mount of UL1678 compliance To use such hardware remove the stand arm unit on the back of the equipment and then attach the hardware to the fitting holes behind the stand arm unit When using the fitting holes on the back of the equipment to install the product do not attach the product directly to a wal
263. t clicking lets you select items in the reverse direction Some functions are available only with the mouse You will need a mouse to access all the functions of the DS L2 The following functions are not accessible without a mouse e Electronic zoom scroll e Screen scroll when split into halves e Histogram display e Pen drawing function e Text input function e Count marking function e Measurement function including calibration e Free straight line drawing function NOTE i When you use an optical mouse be sure to use a mouse pad for optical mice A mouse pad made of cloth with few patterns and dark colors should be used i If a mouse is not recognized disconnect and reconnect it 24 Chapter 6 Basic Operation Method SETU P 1 Preparations Before Photographing 1 1 4 Using a Keyboard You can use a USB keyboard with the DS L2 For frequent text or character entry you will find using a keyboard most convenient Be sure to use a keyboard whose operation is verified Not all USB keyboards are compatible with the DS L2 Alphanumeric characters in upper or lowercase can be used with the DS L2 The function keys on the keyboard can be assigned various functions such as displaying a menu freezing or capturing for quick operations Function key assignments on the USB keyboard F1 CAM menu F2 VIEW menu F3 REC menu F4 TOOL menu F5 F6 MIC menu F7 F8 F9 E ZOON F10 E ZOOM F11 FREEZE F12 CAPTURE Back S
264. t does not comply with the above standard set this to the OFF state Display VGA LCD Color Adjustment VGA Color balance of the external monitor is adjusted LCD Color balance of the internal monitor is adjusted In either of VGA and LCD use R GAIN G GAIN and B GAIN to adjust each of the R G and B colors Mirror Normal H V HV You can flip the image horizontally or vertically or rotate the image 180 degrees Set this function according to the orientation of the camera when attached to a microscope Version Information for Firmware The values written on the lower right show version information for firmware The first three numbers 310 of Ver 310 0208 0195 070221 on the example window mean the version of firmware 1 4 6 Adjustment for Using an External Monitor If the DS L2 is connected to an external monitor you must adjust the monitor output on the Power On Settings screen Monitor output is initially set to 1 024 x 768 resolution 60 Hz XGA If observation with a higher definition display is required when photographing an image we recommend using 1 280 x 1 024 resolution 60 Hz SXGA output NOTE If you select SXGA output images cannot be displayed on the DS L2 s internal display If an external monitor is connected to the DS L2 adjust its output by the procedure described below 1 Connect an external monitor to the DS L2 Refer to Chapter 4 Connecting and Installing the Equipment for details 2 D
265. talling the DS L2 on a flat surface such as a desktop by using the stand arm Extract the stand arm from the DS L2 and place it at an angle of 5 to 10 degrees as shown below Leave a clearance of about 100 mm around the DS L2 to prevent heat from collecting near the equipment When attaching a commercially available arm to the DS L2 Any commercially available VESA standard compliant 75 mm arm UL1678 suited stand and mount can be attached to the DS L2 When doing so make sure the following specifications are met DS L2 weight 1 4 kg Fitting screw hole M4 7 mm deep Tightening torque 80 120 N cm Leave a clearance of about 100 mm around the DS L2 to prevent heat from collecting near the equipment Attach a commercially available arm as follows 1 Remove four fixing screws for the stand arm on the rear side of the DS L2 2 Remove the stand arm from the DS L2 3 Fixa commercially available arm to the DS L2 with four fixing screws When screwing or unscrewing screws use an M4 size Phillips driver Do not use this product alone without the standard stand commercially available arm stand or mount 13 Chapter 4 Connecting and Installing the Equipment 3 Connection Methods 3 Connection Methods 1 Connecting the DS Camera Head Be sure to turn off the power for the DS L2 before making any connections Connect one end of the camera cable supplied with the unit to the CAMERA connector on the DS L2 and the oth
266. the disk drive for the CF card etc and the internal image drive of the DS L2 are each recognized as one removable disk by the PC A screen may be displayed prompting for confirmation of how you want the removable disk to be handled by the OS For the media drive this screen is displayed at the same time media is inserted into the media drive For the CCD image drive because it is labeled NIKONIMG this screen is displayed as shown below The drive name differs with each PC environment In this confirmation screen for either the CCD image drive or the media drive select the Open Folder to View Files Using Explorer check box and the Always Do the Selected Action check box Then click OK button NIKOPNIMG iT Windows can perform the same action each time vou insert Cw a disk or connect a device with this kind of file Pictures What da vou want Windows to do cel using Fhoto Printing wizard View a slideshow of the images using Windows Picture and Fax Viewer XU Copy pictures to a folder on mv computer using Scanner and Camera Wizard y Open folder to view files using Windows Explorer Q Take no action bud When you click OK button the Explorer screen opens showing the content of each drive NOTE In the following cases there might be a conflict between another connected 1 2 3 4 device and the drive letter When media is inserted correctly into position the message Please inse
267. ting OFF If you select ON a time lag will occur in external output with respect to the displayed image on the monitor Be sure to select OFF when you use the remote controller DS RC Otherwise the FREEZE CAPTURE operations may not be done normally Printer Set direct print For details refer to Section 2 3 Outputting Images Directly to a Printer in Chapter 6 35 SETUP 1 4 3 Chapter 6 Basic Operation Method 1 Preparations Before Photographing Image File Settings File Settings Display a menu and select the SETUP tag on it to enter the SETUP menu Select File Settings from the SETUP menu to display the File Settings screen shown below In the File Settings screen set the items indicated below PowerOn Set NetWork Set File Set Additional I HID Mapper Ret to Window File Settings Pref ix Auto Create EN Media Dir MGBOX x Auto FFYYHMDD Sets a folder name in CF USB Memory which files are to be FTP Dir FTPIBOX Auto FFYYMMDD stored max 8 char preF ix 2 Year Month Day Pref ix Num Auto Create File Name DSL2 Auto DDHHMMSS Sets a file name max 4 char Day Hour Min Sec Format Format C FAT32 255 MB Formats an external medium Save Default skip 0 1 3 4 6 g AJ Bj C Dj LAE CIE UR LSU L MJ NI 0 PI QJ S T Vo VEZ When you have finished setting each item press SAVE to s
268. tion Differences among DS Camera Head Models in Chapter 6 4 Abnormal Behavior Symptom Check points The system s internal e If the system is not used for an extended period of time the backup clock does not keep time function deteriorates causing the clock to stop Simply reset the correct correctly time The backup function recovers when the AC adapter is connected to supply the power The keyboard or mouse Be sure to use the keyboard or mouse verified by Nikon does not work or it e Check that only one USB hub is connected operates erratically When using a keyboard that comes with a hub refer to 3 Connecting USB Peripheral Equipment in Section 3 Connection Methods in Chapter 4 for details on how to use a keyboard equipped with a USB hub Cannot write to or read Insert the CF card securely into the connector from a CF card id e Be sure to use a compact flash card Type or Il and a Microdrive Check that you are using a preformatted CF card or one that you ve formatted in FAT12 16 Sometimes these are expressed simply as FAT or FAT32 Depending on the OS used you may not be able to select a format See Section 1 2 Notes on Using an External Recording Medium in Chapter 6 207 5 O Network Chapter 8 Troubleshooting 5 Network Refer to Section 4 2 Network Settings in Chapter 6 for details about network settings Symptom Cannot connect the system to a network
269. ttings and press When using an LCD monitor or an LCD projector Adjust settings on the display side e g on the monitor or projector using the chart incorporated in the DS L2 Follow the procedure described below to make this adjustment 1 Connect an external monitor to the DS L2 2 Turn on the power for the DS L2 Give the unit about 20 minutes to warm up Also make sure the display has time to warm up sufficiently in accordance with the instruction manual 3 Display a menu and select the SETUP tag on it to enter the SETUP menu Select Additional from the SETUP menu to go on to the Additional Settings screen 4 Press the LCD Chart at the lower right corner of the screen A 1 dot pitched monochrome mesh chart will be displayed 5 Adjust the clock phase and clock pitch on the display side Refer to the instruction manual provided with your display for detailed information on performing adjustments Some monitors have an automatic adjustment function 6 Once you have finished adjusting the display click on the BACK button The photographed image is displayed again 1 4 7 Settings for Connecting a Microscope To control the Nikon 80i 90i Upright Microscope or the LV100DA LV ECON LV AF industrial microscope or AZ100M Universal Zoom Microscope or to display its status you need to input a license code For setting of a microscope control license refer to the instruction manual for the microscope control li
270. tton P Focus high sensitivity observation button Exposure button D Image save button Exposure level indicator 1 3Mp 1 3Mi 5Mi Display mode for Fi1 cameras PROG S AE MANU Exposure mode 1 250s Shutter speed C gn Camera sensitivity camera gain io Photometry area m Photometry mode CMP Exposure compensation White balance AE lock Gain Image level adjust Color balance adjust CHRM Color density HUE Hue adjust SFT Black level adjust Contrast fo Jee ee teal Shading correction Noise reduction Sharpness Effect settings Set value clear button 2 Custom mode call 51 CAM E RA Chapter 6 Basic Operation Method 2 Photographing Images a Bo 54 Bos is Cean sec l CHP GAIN 100 c1 c2 c3f e4 eS et c7 Ea e Ses cis RS U Turn OTF sett E No color settings are displayed spa Monochrome cameras Configuration of the Adv2 Advanced2 menu P Focus high sensitivity observation button E Exposure button c Image save button Exposure level indicator PROG S AE MANU F AE Exposure mode Hc E 1 250s Shutter speed C gn 100 Camera sensitivity camera gain PROG MPATE np P g y gain oO Photometry area CHP Photometry mode GAIN v TOO TE TUO E100 v100 R00 B100 CMP Exposure compensation White balance AE lock O SHT Gain Y100 Image level adjust R100 B100 Color balance adjust SFT 00 Black
271. twork groups is to be performed e g sending or receiving data to and from a distant place of business Settings on a PC e When connecting from a PC that is not connected to a LAN check the network settings on the PC first e Set the IP address and other information specific to the PC 208 Symptom Cannot connect the system to a network Continued B Browser Cannot access the camera from a browser Cannot control the camera from a browser Images cannot be saved to an FTP server Chapter 8 Troubleshooting 5 Network Check points Checking for presence on a LAN lt Using a command from the PC to search the DS L2 on a LAN gt Check network connections by using the ping command For Windows select Start Programs Accessories Command Prompt in that order and enter the command in the Command Prompt window For Mac OS X select Macintosh HD Applications Utilities Terminal and enter the command from the TERMINAL screen Example If the IP address of the DS L2 is 192 168 10 2 Enter the command ping 192 168 10 2 and hit the return key If a message such as Reply from 192 168 10 2 bytes 32 time is returned it means that the presence of the DS L2 on a LAN is recognized The problem thus lies in the settings of the DS L2 or the PC If a Request timed out message is returned the presence of the DS L2 is not recognized Check the cables the hub connections
272. u display a menu by right clicking with the mouse the status bar is shown To hide the status bar right click on the bar with the mouse The status bar has three buttons M T and S On each button you can M show hide the main menu T show hide the tool bar or S show hide the shortcut menu 20 Chapter Basic Operation Method S EIU P 1 Preparations Before Photographing BEEF Initial Settings 1 4 1 2 When using the DS L2 for the first time after purchasing you must make the following environment settings before photographing any image 1 Language setting Confirm the menu language is set to English If not set the language to English 2 Date and power save settings Set the unit clock and power save timer 3 Image file save related settings Set names of a recorded image file and a folder in which the recorded image file is saved 4 Startup condition settings Set startup conditions for photography and display states To make these settings display a menu and select the SETUP tag to enter the SETUP menu The correct procedures are detailed below Language Setting Confirm the menu language for DS L2 is set to English Display the menu and select the SETUP tag to enter the SETUP menu When the SETUP menu opens the PowerOn Settings screen appears as indicated below PowerOn Set NetWork Set File Set Additional HID Happer Ret to Window Powe
273. ur system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope with motorized or intelligent nosepiece and the calibration method you selected is using the magnification of the objective and relay lenses you do not need to enter the magnification of the objective If the camera head is connected to the rear port of the DIH you do not need to enter the magnification of the relay lens If you calibrated with one objective other connected objectives will be also calibrated automatically with their magnifications being converted If you selected the using the magnification of the objective and relay lenses calibration method keep in mind that the calibration value may incorporate a certain amount of error attributable to optical errors of the objective With the Nikon 80i 90i microscope connected errors may range up to 10 3 4 2 Calling the Calibration Value To use one of the registered calibration values to perform measurement you must call up the calibration value following the procedure described below before you can use it NOTE Be aware that if you call up a calibration value for any image all of the overlay information for that image will be erased 1 Select the calibration value you want to use from through m7 Drag the mouse pointer over one of the numbers through m7 and the calibration information for that number will be displayed in the Info area for your confirmation NOTE If t
274. urity To improve security make the following settings on the Network Settings screen Be aware that the transferred images and information are not encrypted Limiting logins by User ID and Password settings In the Internal Server set a user ID and a password User ID Input up to 10 characters Pass Word Input up to 10 characters Once you set a user ID you will be asked to provide authentication when connecting to the DS L2 from a browser You cannot log in unless you supply your user ID and password If the user ID is defaulted to anonymous anybody can access your DS L2 via a network NOTE Note that if you save a password as the default word none without changing it the word none will become your password 104 Chapter 6 Basic Operation Method N ETWORK 4 Using the DS L2 via a Network Limiting connectable IPs If you want to limit the IP addresses connectable to the DS L2 select the IP Authority check box To register the IP addresses that are allowed to connect to the DS L2 follow the procedure described below Method for registering the IP addresses that are allowed to connect 1 Insert a CF card or USB memory into the card slot of your computer NOTE A card adapter may be needed depending on your computer 2 Create a directory named NET in the CF card or USB memory 3 Create a text file that contains a list of the IP addresses that are allowed to connect to the DS L2 Description example 192
275. using the shortcut menu refer to Section 2 1 1 Simple Photography Shortcut Menu in Chapter 6 The DS L2 has two kinds of shortcut menus small and large Five and ten shortcut buttons are assigned to the two kinds of shortcut menus respectively You can also assign camera setting functions other than the above to the shortcut buttons There are three kinds of shortcut bars that can be set by the user user1 user2 user3 You can register the shortcut bars using Telnet commands The type of the shortcut menu can be selected in Power On Settings Refer to Section 1 4 5 Startup Condition Settings Power On Settings in Chapter 6 Registering User Settings Shortcut Bar Register the shortcut buttons using Telnet commands w SHMNn k m1 m2 m3 md n 1 2 3 User number k Number of menus up to 12 m1 m2 Menu number Example w SHMN2 4 1 3 7 8 Four menus are registered in the User2 area menu numbers 1 3 7 8 Menus are registered from the left Menu number 1 Save to the media 2 Save to the FTP server 3 Print PictBridge 4 Data reproduction 5 Data deletion valid after reproduction 6 AE lock 7 White balance 8 Freeze pause Zoom co 10 Focus mode 11 Exposure Exposure 12 Capture Capture 13 Scene 1 for industrial use IC wafer 14 Scene 2 for industrial use Metal structure 15 Scene 3 for industrial use Packaging board 16 Other scene 1 Asbestos red e 3t 17
276. uster may activate a personal firewall To prevent this either disable the personal firewall or modify blocking to enable reception via UDP port 12102 which is used by this device For instructions on changing the settings refer to the documentation for your operating system or antivirus software NOTE If ROOMS is not displayed make sure any personal firewall of the operating system or antivirus software is not preventing it 121 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 4 3 4 Java Applet Settings Java applets must be enabled to use ROOMA This procedure varies depending on your browser and operating system For Windows and Internet Explorer 1 Change the browser settings e In Tools select Internet Options 2 Welcome Microsoft Internet Explorer File Edit View Favorites BEES Help m Mail and News gt c ee T 9 Q im lt 4 x Pop up Blocker i bs 3 Manage Add ons Address i 31 30 Addr 2 http 128 31 30 3 5 nice Windows Update Windows Messenger Diagnose Connection Problems Sun Java Console Internet Options e Select Security gt Local intranet gt Custom Level Complete the Microsoft VM setting e Specify Java permsissions select High safety Low safety or Medium safety Complete the Scripting of Java applets setting e Enable scripting of Java applets Welcome Microsoft Internet Explorer File Edit
277. v otic Eupwnaikec XWPEG AUTO TO GUUBOAO UTIOSNADVEL OTLN AMOKOLLON au TOU TOU ripoi vtoq TIPETIEL va yivet EExwploTa Ta k ro80t arteu0Uvovrat vo oe Eupwrtaiouc xprjorec e AuTO TO rtpoi v eival oxe taop vo ETOL WOTE va yiveTal n anokopi r TOU oe setik onpeia Mnv ro rer re paci ue Ta Urt Aorrta aroppippara e ia TEPLOOOTEPEC rnpoqgopieg er kotvovrjore HE TOV iavopu a TOU TIPOlOVTOC rj ue Tic UMEUBUVEC TOTIIKEC apx c yia 0 para oiaxelpiong aroppipup rov s El Symbol oznaczajacy segregowanie odpad w stosowany w krajach Europy Ten symbol oznacza ze produkt musi by wyrzucany oddzielnie Ponizsze uwagi maja zastosowanie tylko dla uzytkownik w z Europy e Ten produkt jest przeznaczony do oddzielnej utylizacji i powinien by dostarczony do odpowiedniego punktu zbieraj cego odpady Nie nale y go wyrzuca z odpadami gospodarstwa domowego e Aby uzyska wi cej informacji nale y skontaktowa si z przedstawicielem przedsi biorstwa lub lokalnymi w adzami odpowiedzialnymi za zarz dzanie odpadami Hul Eur pai orsz gokban rv nes Elk l n tett hullad kgy jt s jelz se Ez a jelz s azt jelenti hogy ezt a term ket elk l n tve kell gy jteni Az al bbiak csak az eur pai orsz gokban l felhaszn l kra rv nyes e Ezt a term ket a megfelel hullad kgy jt helyen elk l n tve kell gy jteni Ne dobja ki h ztart si hullad kk nt e Tov bbi inform ci k rt forduljon a forgalma
278. val and enables to capture the images display the stream screen and control the DS L2 on ROOMS screen Perform the settings of the ActiveX to use ROOMS For the settings refer to 4 3 3 Activex Settings When executing ROOMS the warning may appear as shown below Security Warning Do vou want to install and run http 128 31 30 7 07 L2C TL cab signed on 8 30 2006 3 45 PM and distributed by Mikan Corporation Publisher authenticity verified by YernSign Class 3 Code Signing 004 CA Caution Nikon Corporation asserts that this content ts safe You should only install view this content if you trust Nikon Corporation ta make that assertion O Always trust content from Nikon Corporation More Info Click Install because a digital signature is attached to this program for certification 117 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network 2 3 4 8 5 1 nen suet sree noom noowe on ROOM 2807960 9 The details of 1 to 9 are the same as those of ROOM 1 Refer to the usage for ROOM1 In the Interval Timer marked with 3 MAX can be selected The MAX is not provided to ROOM1 When selecting MAX the preview images are refreshed in real time ROOMS can display images only when the Internet Explorer 6 02 SP 2 or 7 0 browser is used on the Windows 2000 SP 4 XP SP 2 or Vista operating system When accessing the D
279. ve Save 148 Chapter 6 Basic Operation Method USB 5 Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function Using the DS L2 when Connected to a PC by a USB Cable 1 Mass Storage Function BETEM Recommended Operating Environment When the DS L2 is connected to your PC by a USB cable and the mass storage function is performed you can read the recording media inserted in the DS L2 or capture the live image being shot by the CCD camera onto the PC Supported PCs The DS L2 can only be connected to a Windows based However this is limited to PCs that have a USB interface board installed Supported OSs e Windows 2000 Service Pack 3 SP3 or higher e Windows XP e Windows Vista If you are using Windows 2000 be sure to update its version to SP3 or higher Otherwise data may not be read or written to correctly About recording media used by the DS L2 e Usable media Compact flash card Type and II microdrive e Formatting When formatting a medium in your PC make sure it is formatted in FAT12 16 Sometimes these are expressed simply as FAT or in FAT32 Note that depending on the OS used you may not be able to select a format The DS L2 has a function for formatting media The media preformatted in NTFS and so forth that is formats not supported by the DS L2 are formatted in FAT 16 For details on how to use the formatting function refer to Sect
280. ve to In BMP format Save in Nikon v Q 2 e m A My Recent Documents Nikon10 My Documents 9 My Computer File name IMG640 My Network Save as type Bitmap Image 113 NETWORK 2 Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network In TIFF format Save in B Nikon ET A My Recent Documents Nikoni0 My Documents Pr My Computer J File name IMG640 My Network Save as type Choose the folder in the PC where you want to save the image file Enter a file name as necessary and click the Save button When you click the Save button the image that is in the DS L2 is downloaded into the PC Because the latest image is downloaded it may not always be the same as the preview screen NOTE If you click the Cancel button in the folder select screen it is possible that the browser will no longer be updated In such a case wait for a while or temporarily close the browser and reopen it Sometimes the window may be closed while downloading an image file When you open an image file in the DS L2 directly from a PC ona network it is possible that the original window will be closed In that case first download a file to the PC before opening it When using Mac OS to download a BMP file select Save a File to Disk to save the file first and then open it in your application You cannot directly open a download
281. will format a medium when you select a medium to be formatted on the File Settings menu and press the Format icon JO S EIU P Chapter 6 Basic Operation Method 1 Preparations Before Photographing BEEF Menu Configuration and Method of Displaying a Menu 1 3 1 Menu Configuration The menus available with the DS L2 are listed below Adv1 Adv2 Camera setting menus Refer to Section 2 REG1 REG2 Registration menu for camera setting Photographing Images in VIEW Reproduction deletion menu for medium Chapter 6 and Section 7 REC Recording to medium FTP server function Operation Differences among print output DS Camera Head Models in Chapter 6 TOOL Tool menus including cross lines and XY scales Refer to Section 3 Performing MIC Main Menu Registration Registration of grid lines calibration etc Measurements or Entering Information TOOL Menu in Chapter 6 Microscope control menu Refer to the instruction manual When the DS L2 is connected to a Nikon for the microscope control microscope described in Chapter 2 license purchased separately Peripheral Equipment via USB interface a tag for this menu appears To control the Nikon 80i 90i Upright Microscope the LV100DA LV ECON industrial microscope or AZ100M Universal Zoom Microscope or to display its status on the MIC menu you need to input a license code For details refer to the instruction manual for the microscope control license purchas
282. with the units nm um mm or cm If a unit other than these is being displayed set calibration values again You can change the character size by selecting the Font Size check box on the OTHER tab on the Tool menu You can change the line width by selecting the Line Width check box on the OTHER tab on the Tool menu Select highlighted with a red frame the icon on the TOOL menu before changing the color of the axes and moving the axes 98 Chapter 6 Basic Operation Method TOOL 3 Performing Measurements or Entering Information TOOL Menu 3 5 6 Comparing a Translucent Image to a Live Image You can view a translucent image created on the PC while being laid on top of the live image for comparison This function may be used to create a translucent scale or inspect registration of shapes 1 When you press Superimposition images saved in the internal memory are displayed in turn The images are switched in the following manner Superimposition 1 display Superimposition 2 display Press an icon Normal display Press an icon Press an icon NOTE Up to two Superimposition images can be saved in the internal memory Put the images created on the PC in the SIP folder in the CF card and enter the w SIT DT command to save them in the internal memory To run the command w_SIPDT insert either a CF card or a USB memory in the DS L2 T Superimposition data can be cre
283. ws you to switch the state of a photographed image between Freeze and Live CAPTURE button This button has the same function as that for the DS L2 Can be used as a DS Qi1Mc trigger signal However use the DS L2 capture button to save images captured using an external trigger signal For instructions on capture based on trigger signal timing refer to the section on DS Qi1Mc in Chapter 6 Section 7 Operation Differences among DS Camera Head Models SIF a S EIU P Chapter 6 Basic Operation Method 1 Preparations Before Photographing 1 1 3 Using a Mouse You can use a USB mouse with the DS L2 Be sure to use a mouse whose operation is verified Not every USB mouse is compatible with the DS L2 While most normal operations involve left clicking the mouse some may require right clicking For icons to set a value or select a mode left or right clicking the mouse button allows you to increment or decrement a value or select items in the forward or reverse direction e For an icon to increment or decrement a value such as the Gain icon on the CAM menu Left clicking the icon increases the value while right clicking the icon reduces the value e For a shutter speed Left clicking reduces the shutter speed to increase exposure right clicking increases the shutter speed to reduce exposure e For a mode select icon such as one to choose an exposure method on the CAM menu Left clicking lets you select items in the forward direction righ
284. xt to continue Connect the A connector of the USB cable The PC automatically searches for new hardware Click the Install the hardware that manually select from a list Advanced And then click Next 1 Click here 2 Click Next Cancel 174 Chapter Basic Operation Method USB 6 Using the DS L2 when Connected to a PC by a USB Cable 2 Vender Unique Function EE enirar Oar Click the Don t detect will select it from a list And then click Next Please choose your search and installation options Pua a Sy Search for the best driver in these locations Use the check boxes below to limit or expand the default search which includes local paths and removable media The best driver found will be installed 1 Click here Don t search will choose the driver to install 2 Click here Choose this option to select the device driver from a list Windows does not guarantee that the driver you choose will be the best match for your hardware Found New Hardware Wizard Click the Have Disk button Select the device driver you want to install for this hardware Poa tan S Select the manufacturer and model of your hardware device and then click Next If you have a disk that contains the driver you want to install click Have Disk Click the Have Disk button amp This driver is not digitally signed Tell me why driver signing is important Specify C Program Files
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286. ze If the buffer is filled the content is automatically erased and new logs are written to the file beginning with the 0 byte However if transfers to an external server have been set by the w NLFTON command the log data is transferred to the external server before being erased The file name of this log data consists of the letters NL the date months days and hours and the extension txt When transferred at 12 o clock on April 1 the file name would be NL0401 12 txt Telnet commands for logs w NLGON Displays NETLOG w NLGOF Hides NETLOG s NLGCL Erases the content of NETLOG w NLFTON Transfers NETLOG when the buffer is filled w NLFTOF Does not transfer NETLOG when the buffer is filled 105 N ZERO RK Chapter 6 Basic Operation Method 4 Using the DS L2 via a Network Operating the DS L2 from a WEB Browser 4 3 1 Setting the WEB Browser Here Internet Explorer and Netscape are used as examples For instructions on configuring other browsers refer to the browser documentation 4 3 1 1 When Using Internet Explorer 1 Start the PC Refer to your computer s user manual for details 2 Launch Internet Explorer Refer to your computer s user manual or the Internet Explorer help function for details 3 Select Tools Internet Options Left click here to open the pull down menu E about blank Microsoft Internet Explorer Eile Edit View Favorites Tools Help qa is f Mail and News Back K
287. zoom of the digital imaging head while using this function with the Nikon 80i 90i microscope connected to your system the screen display will reflect the zoom magnification Refer to the instruction manual for i Control License Card sold separately If you change the optical zoom while using this function with the Nikon AZ100M microscope connected to your system the screen display will reflect the zoom magnification change accordingly Refer to the instruction manual for i Control License Card sold separately ii If you change the objective while using this function and if the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope using a motorized nosepiece or 97 TOOL Chapter 6 Basic Operation Method 3 Performing Measurements or Entering Information TOOL Menu intelligent nosepiece the screen display will change accordingly Refer to the instruction manual for i Control License Card sold separately If the microscope connected to your system is the Nikon 80i 90i microscope the LV series microscope or the AZ100M microscope using a motorized nosepiece or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through m7 Calibration values are automatically changed when you change the objective Refer to the instruction manual for i

DS Camera Control Unit DS-L2 DS Camera

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