NeoFox Installation and Operation Manual
Contents
1. This is the view looking at the connector on the back of the NeoFox with power plug on the right and the USB plug on the left The connecior is a standard PS 2 connecior Analog Output Parameters List Potential Values 0 Disable analog output NeoFox Viewer Parameter Voltage Output Source 1 Oxygen 2 Temperature not supported 3 Pressure not supported 4 Tau 013 20000 009 02 201510 51 Ocean NeoFox Viewer Parameter Potential Values 5 Alternate O Units Current Output Source O Disable analog output 1 Oxygen 2 Temperature not supported 3 Pressure not supported 4 Tau 5 Alternate O Units Analog Voltage Lower Bound or Voltage lt floating point value gt This floating point value represents Limit Minimum the lower bound of the represented range of the input data Must be lt UBOUND and 2 0 Analog Voltage Upper Bound or Voltage lt floating point value gt This floating point value represents Limit Maximum the upper bound of the represented range of the input data Must be gt LBOUND Analog Current Lower Bound or Current lt floating point value gt This floating point value represents Limit Minimum the lower bound of the represented range of the input data Must be lt UBOUND and 2 0 Analog Current Upper Bound or Current lt floating point value gt This floating point value represents Limit Maximum the upper2. a Jaz stack with the NeoFox Sport module s See your Jaz manual for more information about what ships with Jaz refer to Product Related Documentation SD card containing the neofox cal calibration file NeoFox CD Packing List Additional Recommended Eguipment D oO D DU 0 O NeoFox Viewer Software Temperature Probe FOXY HIOXY or FOSPOR Oxygen Sensor Probe RedEye Patches FOXY HIOXY and FOSPOR FOXY CAL HIOXY CAL FOSPOR CAL This is an optional factory calibration service for environments from 0 to 80 C Bifurcated Fibers 21 02 SMA Splice Bushing This in line adapter connects the probe to the fiber assembly 013 20000 009 02 201510 Chapter 2 Installation Overview There are two steps to completely installing the NeoFox system onto your computer e First you must install the NeoFox Viewer software e The second step is to properly install the NeoFox hardware You must install the NeoFox Viewer software application prior to connecting the NeoFox unit to the computer to install the drivers required for the hardware If you do not install the software first the system will not properly recognize the NeoFox hardware CAUTION Do not connect the NeoFox unit to the PC prior to installing the NeoFox Viewer software Software Installation You can install the NeoFox Viewer software from either the CD that came with your NeoFox unit or from the Ocean Optics website Use the following pr
3. e 12 Single Point Adjusted Calibration Coefficients A0 Al A2 BO B1 B2 CO C1 C2 TO T1 T2 Analog Output Settings Analog Voltage Output Source Analog Voltage Lower Bound Analog Voltage Upper Bound Analog Current Output Source Analog Current Lower Bound Analog Current Upper bound Jaz Communication Enable Jaz Interface Serial and USB Communication RS232 Divisor Latch RS232 Divisor Add Value RS232 Divisor Mul Value RS232 Enable Uart Data Copy Type Uart Data copy Mode Uart Transmission Mode Uart Send Alerts 013 20000 009 02 201510 29 3 Using NeoFox with NeoFox Viewer Software AS Seres Saving Data You can save your NeoFox data in either a fxy file which can be opened in a NeoFox Viewer window or in a csv file which is compatible with Microsoft Excel NeoFox saves data to a disk file until you request it to stop or the application is shut down Procedures for saving both types of files are described below See Data Logging for more information on saving a csv file gt Procedure To save the measurement data in a fxy file 1 Select File Save A window appears displaying a file name with the date and the time the file was last saved 2 Select the desired location to save the file gt Procedure To save the measurement data in a csv file 1 Click the Start Stop Data Logging button from the main screen The Data Logging Control Panel screen appears Data Logging Control Pane
4. 2 Click the Setup tab The Setup screen appears 013 20000 009 02 201510 25 26 Ocean 3 Using NeoFox with NeoFox Viewer Software dr Optics O NeoFox Calibration DEX Setup Two Point Calibration 1 Open Or Start A New Calibration Open L Calibration 2 Select Calibration Method None OZ results wil not be calculated Two Point Calibration Simple calibration using two user entered OZ data points Multi Point Calibration Enter calibration data with many temperature OZ data points Single Point CalibratioiLiser provided temperature O2 data point to recalibrate a multi point calibration 3 Select How Temperature Will Be Measured None Two point calibration only Use NeoFox Temperature Sensor Use A Fixed Temperature Cc 4 Enter Sensor Type And Optional Data 0 000 FOXY Serial 6230 Date 5 6 2009 v HIOXY FOSPOR Notes Additional Optional Notes 5 Enter Calibration Data In The Selected Tab 6 Save Calibration Data And Download To Board I Save J Calibration Download S OK Under Select Sensor Calibration Method choose Two Point Calibration Under Select How Temperature Will Be Measured choose None Two point calibration only Under Enter Sensor Type And Optional Data choose the type of sensor and add the serial number and any notes needed Select the Two Point Calibration tab You need two known concentrations of oxygen to perform this cal
5. Clear All E y y Data j Automatic X Oxygen Ki This screen s controls enable you to perform the following actions e Insert comments into the exported data file using the Enter New Event textbox See Entering a New Event for more information e Set scans to average e Select the sensor type e Select Automatic or Manual Gain e Start Stop Data Logging see Data Logging 013 20000 009 02 201510 15 3 Using NeoFox with NeoFox Viewer Software AS Seres Entering a New Event The Enter New Event text box at the top of the screen enables you to insert your comments into a file containing exported data Your comment will appear in the Events column at the far right end of each row of data You may enter multiple comments Each comment is time stamped with the instant you clicked on the Enter New Event field When you create the export data file your comment s will appear on the row of data that most closely corresponds to the time you created your comment gt Procedure To enter a new comment 1 Click the Enter New Event field at the top of the screen 2 Enter your message into the text field 3 Press the Enter key Data Logging You can save a csv file useful for Microsoft Excel via data logging When you click the Start Stop Data Logging button on the main NeoFox Viewer screen the Data Logging Control Panel screen appears Data Logging Control Panel Start Loggi
6. NeoFox Phase Fluorometer Installation and Operation Manual For Products NeoFox GT and NeoFox Sport Document 013 20000 009 02 201510 A HALMA COMPANY AMERICAS amp WORLD HEADQUARTERS Phone 1 727 733 2447 Ocean Optics Inc Fax 1 727 733 3962 830 Douglas Ave Dunedin FL 34698 Sales info oceanoptics com USA Orders orders oceanoptics com o Support techsupport oceanoptics com Manufacturing amp Logistics 4301 Metric Dr Winter Park FL 32792 USA EUROPE MIDDLE EAST amp AFRICA gt o Phone 31 26 319 0500 Sales amp Support Fax 31 26 319 0505 Geograaf 24 Email info oceanoptics eu 6921 EW Duiven The Netherlands Germany 49 711 341696 0 UK 44 1865 811118 Manufacturing amp Logistics France 33 442 386 588 Maybachstrasse 11 73760 Ostfildern Germany ASIA Fo ge 0 Ocean Optics Asia ax 8621 666 Gubei Road Email asiasales oceanoptics com Kirin Tower Suite 601B Japan amp Korea 82 10 8514 3797 Ee DEU Et Shanghai PRC 200336 www oceanoptics com Copyright 2010 Ocean Optics Inc All rights reserved Trademarks All trademarks within are the property of their respective owners Limit of Liability Every effort has been made to make this manual as complete and as accurate as possible but no warranty or fitness is implied The information provided is on an as is basis Ocean Optics Inc shall have neither liability nor responsibility to any person or entity with res
7. itself It will not charge the battery or provide power to the DPU Very few users will ever need to use the NeoFox module s auxiliary power connector 3 Connect the NeoFox unit to your computer using the USB cable See Installing NeoFox Hardware Drivers Note On the NeoFox Sport hardware there is a difference between the USB connector on the DPU module and the USB connector on the NeoFox module The USB connector on the NeoFox module is the NeoFox Sport s primary USB connector When the USB cable is attached to the NeoFox module and the stack power on you can interact with the NeoFox module through the NeoFox Viewer software The USB connector on the DPU is the unit s auxiliary USB connector You will not be able to interact with the device in NeoFox Viewer if you have plugged the USB cable into the DPU module You will only want to use this USB port if you need to update the DPU firmware 4 Connect the temperature probe if you have one to the rear panel of the NeoFox unit 5 Locate the bifurcated fiber that came with the system This optical fiber assembly has a Y shaped design 6 Connect one arm it doesn t matter which one of the bifurcated end of the probe fiber to LED connector and the other arm to the Detector connector on front of unit 013 20000 009 02 201510 9 Ocean Sem 7 If you are using an Oxygen probe locate the 21 02 SMA Splice Bushing that came with the probe This item is a 0
8. 2 Hioxy 2 FOSPOR Gain Automatic Manual APD Voltage 1 5S l 8l rt LED Rate KHz 6 68 Setup Status Advanced Status Advanced Settings 2 Click the dik i button in the Gain field 3 Slide the APD Voltage selector to the desired level 4 Click the EELER button in the Gain field Calibrating the Probe When you access the software for the first time the Oxygen field will be blank To see an oxygen value you must first enter calibration information for the probe This calibration information is unigue for each oxygen sensor probe itself but is independent of the NeoFox hardware unit Therefore a new calibration must be loaded each time a new sensor is added or before first use You have the option of purchasing a factory calibration for your Oxygen Sensor Probe FOX Y CAL HIOXY CAL FOSPOR CAL If you have a factory calibration follow the instructions for Multipoint Calibration If you do not have a factory calibration follow the instructions for a Two Point Calibration Multipoint Calibration Follow these instructions if you purchased a factory calibration file for your sensor This file can be found on the NeoFox CD To retrieve it select Calibration from the Options menu gt Procedure 1 From the Main screen select Options Calibration 013 20000 009 02 201510 23 Ocean 3 Using NeoFox with NeoFox Viewer Software dr Optics 2 Click the Setup tab The Setup screen appears j
9. NeoFox Oxygen Sensor Probes The NeoFox is used with Ocean Optics NeoFox software and custom probes priced separately Ocean Optics oxygen sensor probes are low power portable devices that offer high sensitivity reversibility and stability Their small size is useful for remote monitoring What s more the thin film used in the probe tips consumes no oxygen allowing for continuous contact with the sample Ocean Optics oxygen sensors offer other key advantages they re ideal for viscous samples and are immune to interference caused by pH change or from changes in ionic strength salinity and biofouling The NeoFox supports the following Ocean Optics sensors e FOXY Ocean Optics standard oxygen sensors designed for monitoring oxygen partial pressure in gas and aqueous solutions FOXY is a fiber optic fluorescence probe with proprietary oxygen sensing thin film coating on the tip 2 013 20000 009 02 201510 Ocean haaf K ti e HIOXY Designed for monitoring oxygen partial pressure in nonaqueous vapors and solutions The sensor coating chemistry is compatible with oils alcohols and hydrocarbon based vapors and liquids e FOSPOR A new generation of highly sensitive sensor coating for monitoring traces of oxygen in gas and liquids See http oceanoptics com product category fibers and probes for more information on probes available from Ocean Optics The NeoFox connects to a PC via USB connection and saves your d
10. bound of the represented range of the input data Must be gt L Analog Output Specifications Name Min Typ Max Conditions 4 20 mA Loop Voltage 11 V 24V Rioop 2 Q 4 20 mA Output Accuracy lt 160 pA 25 C Vioop 12V Rroop 2 Q 0 5V Output Accuracy lt 50mV 25 C 0 5V Output Resistance 2500 Interfacing to the 4 20mA Output The NeoFox includes a non isolated 4 20 mA current sink that can be used to transmit oxygen temperature or tau data It is extremely important that you set up the interface for this output correctly or else irreparable damage to the NeoFox unit can occur 52 013 20000 009 02 201510 Ocean hd RESE emt The figure below shows a complete 4 20 mA loop and its 3 main components the NeoFox unit an ammeter and a voltage supply In the diagram the NeoFox unit is shown on the left and the customer s interface circuitry voltage supply and ammeter is on the right Although they are drawn close together the NeoFox unit and the customer s interface circuitry may be physically located far apart connected by a 2 wire cable Loop Voltage Supply Neofox Unit Ammeter Typical 4 20 mA Interface 4 20 mA Loop Voltage Supply As a simple current sink NeoFox does not provide its own power for the current loop The user must provide an isolated power supply between 11 V and 30 V measured at the input to the NeoFox unit to
11. i NeoFox Viewer Setup Welcome to the NeoFox Viewer Setup Wizard The Setup Wizard will install NeoFox Viewer on your computer Click Next to continue or Cancel to exit the Setup Wizard Cancel 3 Click Next The Select Installation Folder screen appears The default location is C Program Files Ocean Optics NeoFox Viewer 013 20000 009 02 201510 Ocean e dr Optics 2 Installation i NeoFox Viewer Setup Select Installation Folder This is the folder where NeoFox Viewer will be installed Folder c Program Files Ocean Optics NeoFox Viewer Advanced Installer 4 Click Next to select the default location or browse to the desired location and click Next You are now ready to install the software ig NeoFox Viewer Setup Ready to Install The Setup Wizard is ready to begin the NeoFox Viewer installation Click Install to begin the installation IF you want to review or change any of your installation settings click Back Click Cancel to exit the wizard Advanced Installer 5 Click Install The Neofox Viewer installs at the selected location This process can take several minutes When the software has successfully installed the final Install Wizard screen appears 013 20000 009 02 201510 7 Ocean CG NeoFox Viewer Setup A Completing the d NeoFox Viewer Setup Wizard Click the Finish button to exit the Setup Wizard Finish 6 Click Finish Go to H
12. port on the NeoFox module not the DPU and the computer Start the NeoFox Viewer software See Chapter 3 Using NeoFox with NeoFox Viewer Software Perform a calibration See Calibrating the Probe Select the sensor type as appropriate im MR Verify that the calibration has been downloaded to memory by cycling power to the device and then taking a measurement Note Once you are in the field you will not be able to update this calibration However you will be able to do a Single Point Reset 36 013 20000 009 02 201510 Ocean gp Optics The following additional steps must be taken once after a flash reset has been performed These steps are required to let the device know that it should communicate with the NeoFox Sport DPU 4 Using NeoFox Sport gt Procedure 1 In the NeoFox Viewer software Select View Advanced Settings 2 Click on the Advanced Settings tab in the lower right hand corner of the screen g NeoFox Viewer File View Options Window Enter New Event C Ocean Optics NeoFox NF_QMS24 x i Trends E Sensor Waveform Oxygen Tau usec Scans to Average Blue Red Both OAvgd Raw a 10 Apply Stop Sensor Data Red LED Temperature C Data Export Interval Clear Al 53991 AD Data EI milliseconds o LED Cycle Timing o 1 Sensor Waveform Decay Wi Advanced Settings E Algorithm Settings LED Avg Cycles
13. power the loop This power supply must be completely separate from the NeoFox and its power supply the grounds of the Neofox and the supply cannot be connected together Most customers will use separate AC adapters to power their NeoFox unit and their interface circuitry Usually this will result in the two circuits floating with respect to each other so that the circuit will work properly However it is important to note that NeoFox has a grounded metal case that is connected to its circuit ground and its USB shell Hence customers who connect the NeoFox s case ground to their interface circuit s case ground will find that the measurements are not correct Some common situations that could lead to unintended ground connections are the following e Mounting both devices on a common metal surface such as a DIN rail e Connecting both devices to a single computer or USB hub e Using the same measurement device to measure the 0 5V output and the 4 20mA output The 0 5V output is referenced to the NeoFox circuit ground e Using the same measurement device to communicate with NeoFox via RS232 and to measure the output of the 4 20 mA output RS232 is only available in NeoFox GT models with an F or greater in the third digit of its serial number which are available by request 013 20000 009 02 201510 53 Ocean Sem Users should be aware of any possible connections between NeoFox and their interface circuits when setting up their hardwa
14. rare ee ee ee ee ee ee 8 InstallingNeoFoxHardwarebDrivers A e e gt gt gt Zm 10 Installing Hardware Drivers Using Windows Vista m a 10 Installing Hardware Drivers Using Windows AP 12 Chapter 3 Using NeoFox with NeoFox Viewer Software 15 DIVINO dE Eh aida dE Ge EE EM CM SE s 15 Entering TEE 16 Data Bo o o UE 16 Advanced Setting EE 17 EE eebe ege EE Eeer 18 EI 19 013 20000 009 02 201510 OE dy Or ICs Table of Contents Advanced KEE TEE 20 Advanced Settings EE 20 eee gez rg E Eege 22 APD Voltage sms S Pe e ee ee eee 22 Galkraindgif fFiB ss sd sad nes 23 Multipoint er dle io EE OE EE ER OO Loser tie 23 Two Point Calibration missa sk oe De sg Do ENEE ges DEENEN 25 Saved Seilis se N eee ee A ee EE ee S 28 er liede EE EE OE EE sj 30 NeoFoxCommunicationinterfaces 31 Saving Restoring Chart Layouts ke 31 Saving KE ite EE 31 Resetting Saved Settings nte ee GR AA ee near Re ee ee ee aeee nana ee AR ee 32 Chapter 4 Using NeoFox Sport es RR Re RE RR RR RR EER ER RR RR ERGE EE RR RR 33 SR RA A ON EA EE OE OK 33 NGOFOX Sport Features a idas ee aa e 33 NeoFox Sport Menu EE 34 Setting Up th slae doele neren 36 Data Logging Saving D l ss crest cise EG EE EE a ein bal SSL ra 37 LOGGING Datel TE 37 ViewingtheDatalogFileName AMAMAMMA e gt gt A gt mZm m AR ee AR Re ee AR ee ee ee ee ee ee
15. resistor at 20 mA would have a 2 volt drop across it If the power supply was 12 V then the loop voltage would actually measure under 10V and the system would be outside of specification 11V is the minimum specified loop voltage It is important to select a resistor that will not subtract too much from the loop voltage 54 013 20000 009 02 201510 Ocean RESE rom Resistor Voltmeter Voltmeter and resistor used to measure current in the loop 013 20000 009 02 201510 55 Ocean Analog output ND OPIIS 56 013 20000 009 02 201510 Appendix D Calculating Drift Per Sensor Drift differs depending on the sensor formulation FOXY FOSPOR or HIOX Y See the table and example below Stability Continuous LED FOXY Formulation FOSPOR Formulation HIOXY Formulation Lifetime Stability 0 0006 usec HR 0 003 usec HR 0 0002 usec HR Tau Oxygen Stability 0 01 HR 0 005 HR 0 007 HR Example Calculate drift for a FOXY sensor probe while measuring a sample every 2 5 hours for 100 hours with the LED being lit for 1 minute New drift reported drift duty cycle on duty cycle off New drift 0 01 hr 1 min 2 5 hr 60 min hr 2 5 60 converts hours into minutes so as to cancel out the dimensions New drift 0 01 hr 1 min 150 min New drift 0 01 hr 0066 New drift 0 000066 hr To run the experiment for 100 hours New drift 0 000066 hr 100 hrs New drift 0 006 013 20000 009 02 20
16. the screen The curve fitted graphs appear on the right side of the screen 24 013 20000 009 02 201510 dy Optics 3 Using NeoFox with NeoFox Viewer Software Calibration Data Temp C Oxygen Tau wc ac 2c ic Ox D 0 0 1 945 0 10 1 515 o 20 1 285 0 30 1 135 o 40 1 027 10 0 1 893 10 10 1 445 2 10 20 1 215 Fig 5 10 30 1 069 i 10 40 0 965 B F 16 20 0 1 842 E 2 20 10 1 376 E 3 20 20 1 147 E 20 30 1 002 20 40 0 9 30 0 1 775 sl Computed Calibration Constants A B C A 000015508 0 2529788 73 060908 20 OZ Concentrations B 0 00173631 0 9068780 118 00368 C 0 0018949 1 16128986 191 2017 T 4 5714285 0 02070228 0 300387 Curve Fitting inear O Quadratic Calculate Options d Calibration Save Tau MOC sl Kgl Download S OK Calibration een BG Hp GE Se 1 Calculate 8 Click on button on the bottom right corner of the screen The Computed Calibration Constants table fills in on the bottom left corner of the screen Download 9 Click the button to use the coefficients for oxygen calculation A Download Confirmation screen appears 10 Click Yes to confirm the download and continue Two Point Calibration If you want to do your own calibration instead of using a factory calibration follow these Two Point Calibration instructions gt Procedure 1 From the Main screen select Options Calibration
17. whatever units are currently selected Oxygen Unit Label The oxygen unit label either ppm Torr or um L Algorithm Temperature The temperature that was used to perform the conversion from Tau to Oxygen If the NeoFox s temperature source parameter is set to Sensor temp then this is the sensor temperature Fixed temp then this is the fixed temperature value None then this is 200 Sensor Temperature The sensor temperature in degrees C Tau The tau value in microseconds Algorithm Pressure The temperature that was used to perform the conversion from partial pressure of Oxygen to concentration or mole If the NeoFox s pressure source parameter is set to 38 013 20000 009 02 201510 yOcean hd SR ei Sensor Pressure then this is the sensor pressure Fixed Pressure then this is the fixed pressure value None then this is 101 325 Sensor Pressure The pressure in kPa NeoFox Sport Saved Settings Most of the parameters that can be set through the NeoFox Sport e user menus can be saved to the NeoFox Sport module s flash memory which allows those settings to be restored the next time that the device is powered on See Saved Settings for a list of settings that can be saved To save the flash write settings navigate to the Sensor Setup Save Settings menu Note that this saves ALL of the savable parameters in their current state Also performing a Single Point Reset through the D
18. you to set the source of the temperature either none the sensor temperature or a fixed temperature in degrees Celsius Default current value of fixed temperature variable Range 0 0 200 0 C Pressure Allows you to set the source of the pressure either none the sensor pressure or a fixed pressure in kPa Default current value of fixed pressure variable Range 0 200 kPa Averaging Set the running average of the measurements Default current value of variable Range 1 300 Duty Cycle Set the duty cycle time in seconds Range 1 999 Set Off time to 0 to make it always run Flashing On Off Set the flashing status of the probe LED 013 20000 009 02 201510 35 Ocean Sem 4 Using NeoFox Sport Menu Item Description Save Settings Select Accept to save your settings or Cancel to discard them Sensor Info Displays sensor information module ID FPGA controller and PCB revision level Setting Up the NeoFox Sport Before using the NeoFox Sport in the field you must download the appropriate sensor calibration to the NeoFox device This must be done with your NeoFox device connected to a computer gt Procedure 1 Connect the power supply to the DPU power jack not the NeoFox auxiliary power jack AC Power Outlet DPU Power Connection Power Cord Press the red power button O to turn the device on Ee Connect the USB cable between the USB
19. 1510 57 Ocean D Calculating Drit Per geren BOR S 58 013 20000 009 02 201510 A advanced settings 17 Advanced Status tab 20 analog current boundaries 50 analog data range selection 51 analog output 47 connector 47 parameters 51 pinouts 51 saving settings 51 specifications 52 Analog Output configuring current output channel 48 configuring voltage output channel 47 analog voltage boundaries 50 APD voltage 22 applications 3 B battery detector 39 C calibration 4 multipoint 23 probe 23 two point 25 chart layout restore 31 save 31 Communication Interfaces 31 D data saving 30 data log file format 38 data logging 16 NeoFox Sport 37 013 20000 009 02 201510 Index data range selecting 50 data source selecting 49 document audience iv purpose iv summary iv E enter new event 15 Enter New Event field 16 equipment included 3 recommended 4 event entering new 16 F features 2 NeoFox Sport 33 fiber 4 firmware updating 43 FOSPOR 3 FOXY 2 hardware 8 hardware drivers installation 10 Windows Vista 10 Windows XP 12 HIOXY 3 Install from CD 6 59 Index from Web 6 installation hardware 8 hardware drivers 10 software 5 isolation 4 20 mA loop 54 L log 16 loop voltage supply 53 menu NeoFox Sport 34 menu description NeoFox Sport 34 N NeoFox overview 1 NeoFox Sport 33 data logging 37 features 33
20. 38 Setting the Data Logging Inten 38 Data Log File ae N da N N N li 38 NeoFox Sport Saved Selos EE IE DE ead ED GEN dd 39 Low Battery Detector AE OR ER EE 39 Appendix A SpecificationS ss Re RR RR RR EER EE RR RR RR EE RR RR RR Ee RE EE RR RR 41 Appendix B Maintenance s ER RR RR RR ER RARR ER EE RR RR ee Ee 43 Updating al EE EE EE N EE EE e EEEE ENEAN 43 Updating Software css ss asais 43 Reen e die DE 43 Appendix C AnalogOutput 0 000 enses Ee EEN 47 Analog Kette nl e 47 Configuring Analog Voltage Output Channel with NeoFoxViewer 47 Configuring the Analog Current Output Channel with NeoFox Viewer 48 Selecting a Data SUr e HA RE ER N OE RE AE 49 Selecting a D t Ran d ME EE OE N EE aan zada 50 Saving the Analog Output Settings iss add 51 013 20000 009 02 201510 iii Table of Contents Analog Output Pinouts Bee 51 Analog Output Parameters D E 51 Analog Kelte lee E 52 Interfacing to the 4 20mA Output 52 4 20 mA Loop Voltage EEN 53 4 20 E 54 Resistor Volt Meter ass sasn 54 Appendix D Calculating Drift Per Sensor 57 Vie AE ARDE DES EE ss 59 013 20000 009 02 201510 About This Manual Document Purpose and Intended Audience This document provides the users of the NeoFox and NeoFox Sport with instructions for setting up calibrating and performing experiments w
21. 7 APD Voltage 20 4 20 1 0 400 us K oO Gain x 0 300 us ds LED WEET mm 0 100 us 0 000 us NeoFox Viewer Advanced Settings Window Note If the controls on the right side of the screen seem to be disabled stuck or if they say Error even after clicking the Manual Gain button EE the problem is likely that the firmware and software versions are not compatible You will then need to install the appropriate software version onto your computer to use NeoFox See Software Installation and Appendix B Maintenance for more information The Advanced Settings window contains the following tabs bottom right corner of screen e Setup e Status e Advanced Status e Advanced Settings Setup Setup allows you to perform such functions as Sensor Type Selection adjusting the APD Voltage for firmware versions below 4 00 and Calibrating the Probe 18 013 20000 009 02 201510 ae Optics 3 Using NeoFox with NeoFox Viewer Software B NeoFox Viewer EE Ele View Options Window Enter New Event O Ocean Optics NeoFox NF_QMS24 x Trends Scans to Average Blue Red Both GAvgd Raw io Sensor Data Red LED Data Export Interval 100 milliseconds LED Cycle Timing o e 4 2sus Sensor Waveform Decay Waveform FFT LED Rate KHz Setup Status Advanced Status Advanced Settings Status Clicking on the
22. 75 screw with two female ends Screw one end of the splice bushing into the SMA 905 connector on the end of the probe If you are using the RedEye Patch you don t need the splice bushing 2 Installation Installing NeoFox Hardware Drivers When you connect NeoFox to a computer for the first time using the USB cable the hardware drivers should install automatically for Windows 7 8 8 1 and 10 if you installed the NeoFox Viewer software first If you are using a Windows Vista or Windows XP computer follow the appropriate instructions below to install the drivers Installing Hardware Drivers Using Windows Vista The following Found New Hardware wizard appears when you first attach NeoFox to computers running the Vista operating system 10 013 20000 009 02 201510 2 Installation Windows needs to install driver software for your Ocean Optics NeoFox Locate and install driver software recommended Windows will guide you through the process of installing driver software for your device Ask me again later Windows will ask again the next time you plug in your device or log on Don t show this message again for this device Your device will not function until you install driver software gt Procedure 1 From the first Vista hardware wizard screen select Locate and install driver software recommended The next hardware wizard screen appears 2 Select Don t search online The next hardware wizard screen a
23. Blue LED Prog Gain Ist Stage Gain Blue ee ee 10 0 146 Disabled 100 Yo OFF OFF 0 0 0 H Gain 8x Gain 40x Diagnostics LED Diagnsotics LED Setup Status Advanced Status Advanced Settings NeoFox Viewer provides you with the capability to change the salinity correction setting from its default setting of 0 Salinity correction applies to dissolved oxygen for PPM and uM L it does not apply to percentage gt Procedure To change the salinity setting 1 Double click on the Salinity Calibration value default is 0 2 Type the new salinity value 3 Press Enter 013 20000 009 02 201510 21 C Ocean 3 Using NeoFox with NeoFox Viewer Software dr Optics E NeoFox Viewer File View Options Window Help Enter New Event O 8 20 2010 1 38 PM x O Ocean Optics NeoFox NF 0046 x Trends Decay Waveform Oxygen Tau usec Signal Scans to Average Blue Red LED v M Show Phase Log View Slel E 200 Apply m Stop m ge Decay Waveform tem Temperature C 4 Max 11875 Clear All Mad ji Multipoint To i 6 6346176E 05 Select and Type Multipoint T1 0 03121346 New Value Here Multipoint T2 E 0 1552405 E tion Host Settings Data Export Interval 1 Std Dev Length seconds 5 Data Export Interval Units 1 E LED Timing LED Clock Divider 1 LED On Time 128 LED Cycle Time 256 cycles LED Clock
24. M e Gaseous oxygen partial pressure in Torr e Dissolved oxygen partial pressure in ppm parts per million e Dissolved oxygen concentration in um L micromoles per liter e Gaseous oxygen concentration as a percentage of total pressure in Mole e Atmospheric pressure from the on board pressure sensor in units kPa e Data logging of temperature oxygen and pressure readings in units listed above e In field single point reset also available on desktop NeoFox 013 20000 009 02 201510 33 com 4 Using NeoFox Sport NeoFox Sport Menu Oxygen Sensor A Sensor Readout Prs kPa PPO2 PPM Torr pmol L or Mole Temp C Tau us Sensor Setup O2 Sensor Setup Single Pnt Reset SPR Mole Ready to Sample ii gi SPR Temp C SPR Complete X Cancel Data Logging Datalogging A Turn datalog on off Accept Datalog interval X Cancel e Datalog filename X Cancel Oxygen Units PPO PPM Torr pmol L or Mole Temperature Temperature Fixed temp value Pressure Fixed pressure FIXED PR Pi Fixed pres value Dari X Cancel e Duty Cycle X Cancel VAccept Accept XCancei Flashing On Off A Flashing on Save Settings X Cancel e Sensor Info Menu Tree Description 34 013 20000 009 02 201510 OE dr Opi ICS 4 Using NeoFox Sport Menu Item Descriptio
25. NeoFox devices with updated firmware In Firmware Releases 2 03 and greater the HIOXY probe s automatic frequency setting has been changed from 100 KHz to 45 KHz Using HIOXY probes calibrated at 100 KHz will produce incorrect results Using older NeoFox units with new calibrations will also produce incorrect results If your HIOXY probe was calibrated before 01 SEPT 2009 return it to Ocean Optics for a full recalibration at 45 KHz Or you can use firmware version 2 02 available from Ocean Optics Or you can enter the frequency setting manually although this is not recommended The temperature probe reading appears erratic or is drastically incorrect Known issue with units manufactured prior to August 2009 Contact Ocean Optics Technical Support The waveform windows or settings status windows are displayed as popups instead of being docked on the side of the Main window N A All utility windows can be docked or undocked by double clicking the title bar of the utility window 013 20000 009 02 201510 45 B Maintenance 46 Ocean ES ics 013 20000 009 02 201510 Appendix C Analog Output Analog Output Connector The Analog Output connector is located on the rear panel of the NeoFox unit Analog Output Connector There are two user programmable analog outputs available on the NeoFox as follows e 4 20 mA current source e 0 5V voltage source Analog thres
26. PU menu may result in all of the savable parameters being overwritten Low Battery Detector The NeoFox Sport contains a low battery detector that prevents inaccurate readings in low battery conditions When the device detects that battery conditions are too low for reliable operation its tau and oxygen measurements will be set to O or 1 NeoFox disables its measurements shortly before the device itself turns off 013 20000 009 02 201510 39 Ocean Ise NeoFox Sport OPS 40 013 20000 009 02 201510 Appendix A Specifications Specification Value Parameters Measured Gasseous oxygen partial pressure dissolved oxygen concentration temperature pressure via onboard pressure transducer Control Software Windows 2000 XP Vista 32 and 64 bit and Windows 7 32 bit control software with data logging capability controls include modulation frequency data rate signal averaging APD gain analog gain LED intensity Sampling Rate Acquires data samples every 0 1 second NOTE This is not the response speed of the probe just the rate at which the probe is digitally sampled Thermistor Probes Closed end stainless steel tube with thermistor sensor mounted in tip liquid immersible rugged design 1 8 NPT fitting temperature range 0 to 75 C Pressure Measurement On board pressure transducer monitors atmospheric pressure Input Power Approximately 2 5W 5V Communications USB 0 5V anal
27. Phase Avg Count Blue Baseline Phase rad LED Recovery Cycles Disable Red LED Disabled Phase Avg Count Red 100 Oxygen Units E Analog Output Voltage Output Source Current Output Source Voltage Minimum Limit Voltage Maximum Limit Current Minimum Limit Current Maximum Limit E Analog Settings Blue LED Prog Gain ist Stage Gain Blue AE Diagnostics LED Diagnsotics LED Setup Status Advar us advanced Settings 4 Scroll down to the Enable Jaz Interface parameter and set it to Enabled 5 Set the Flash Write parameter to Enabled It will remain displayed as Enabled briefly before reverting to Disabled Data Logging Saving Data NeoFox Sport provides you with the following functionality for logging saving data e Log data to an SD card e View the file name of the data log e Set the data logging interval Logging Data The NeoFox Sport allows you to log data to an SD card via the Oxygen Sensor Sensor Setup Data Logging menu When you select Turn datalog on from this menu a log file is created on the SD card 013 20000 009 02 201510 37 Ocean bier SE and NeoFox Sport begins logging data automatically The name of this log file is automatically generated when the data log begins in the format nf xxx tsv where xxx is a 3 digit number for a filename that does not already exist in the NeoFox directory 4 Using NeoFox Sport When you select Oxygen Sensor Sensor Setup Data Logging
28. Selection 46 875 KHz Get LED Low Only Disabled E Misc Settings Diagnostics LED Enabled Flash Reset Disabled E Salinity Calibration Salinity Calibration Setup Status Advanced Status advanced Settings Sensor Type Selection Before using the software you must select the appropriate sensor type from the three buttons in the Sensor field on the right side of the screen Choose FOXY HIOXY or FOSPOR APD Voltage APD voltage is the voltage applied to the APD detector which determines the detector s sensitivity towards converting photons to electrical signal This can be manually adjusted for firmware versions below 4 0 if the signal level of the sensor appears to be low For firmware versions 4 00 or above this parameter is set at the factory and cannot be changed gt Procedure To manually adjust the signal level 1 From the Basic View screen select View Advanced Settings to display the Advanced View screen Make sure that the Setup tab lower right corner is selected 22 013 20000 009 02 201510 we opie 3 Using NeoFox with NeoFox Viewer Software Ele View Options Window Enter New Event O Ocean Optics NeoFox NF_QMS24 x Sensor Waveform Tau usec Signal Scans to Average Blue Red Both Avgd Raw Temperature C Data Export Interval 100 milliseconds D LED Cycle Timing o 1 sensor Waveform Decay Waveform FFT Setup Sensor Foxy
29. Status tab displays the status information shown below O NeoFox Viewer Ele View Options Window Enter New Event O Ocean Optics NeoFox NF_QMS24 x 22 000 o LED Cycle Timing o EES 25us Sensor Waveform Decay Waveform FFT 7 Status Temperature C PCB Sensor Setup Status Advanced Status Advanced Settings 013 20000 009 02 201510 19 a an 3 Using NeoFox with NeoFox Viewer Software dy Optics Advanced Status The Advanced Status tab displays the values of parameters such as Oxygen base and converted Tau etc as shown below Bl NeoFox Viewer File View Options Window Enter New Event O Ocean Optics NeoFox NF QMS24 x i Trends 3 Sensor Waveform Oxygen Tau usec Scans to Average Blue Red Both Avgd 10 Apply es Stop Sensor Data Red LED Temperature C Data Export Interval 100 milliseconds Clear All AD Data a LED Cycle Timing a Sensor Waveform Decay Waveform FFT Parameter Value Comment CS MM es Ye Oxygen Converted Calculated 02 in alt Tau Phase 0 1144 usec Calculated TAU P Tau Phase StdDev 0 27 Tau phase method Tau Precision 0 001439 Tau Precision Tau Tau Curve 0 0000 Calculated TAU C Tau Host Calculated 0 1144 Tau calculated by BLUE Decay Time o Waveform decay ti BLUE Phase 18 554 0 324 Average
30. Turn data log off then the file will be closed A new file is created the next time you select Turn data log on Viewing the Data Log File Name You cannot set the file name but you can view the name of the current log file by selecting Oxygen Sensor Sensor Setup Data Logging Data log filename Setting the Data Logging Interval You can also set the data logging interval via the Oxygen Sensor Sensor Setup Data Logging Data log interval menu selection This can be used to reduce the size of the log file The minimum data log interval is 1 second Data Log File Format The datalog file is formatted as a tab separated value file This file can be easily imported into Microsoft Excel or comparable spreadsheet programs A single sample in the data log is formatted as follows millisecond count lt tab gt upload date lt tab gt upload time lt tab gt oxygen lt tab gt oxygen unit label lt tab gt algorithm temperature lt tab gt sensor temperature lt tab gt tau lt tab gt algorithm pressure lt tab gt sensor pressure Where Millisecond Count The millisecond counter of the NeoFox device at the time of the actual sample Because this value is stored at the actual sampling time this value is a more reliable timing metric than the upload date and upload time fields Upload Date Upload Time These fields represent the date and time that the sample was uploaded to the DPU Oxygen The oxygen measurement in
31. ability and sensitivity to drift is important and where sample set ups must be left undisturbed for long periods of time NeoFox NeoFox is available as a compact self contained benchtop unit 013 20000 009 02 201510 1 Introduction NeoFox Sport NeoFox Sport is available as a handheld oxygen sensing system with a phase fluorometer an onboard microprocessor with OLED display and a battery module or as part of the Jaz community of stackable modular and autonomous component modules limit of one sensor module per stack Features NeoFox is a fluorescence sensing detector for our optical sensors line and provides a viable alternative to traditional chemical sensing devices Our optical sensors consist of transducer materials applied to the tips of optical fibers or to substrates such as patches or cuvettes which change optical properties in response to specific analytes in their immediate environment Additional features that are available to NeoFox Sport users include e Battery powered operation e Real time display of temperature and oxygen in various measurement units e Gaseous oxygen partial pressure as a percentage of 1 ATM e Gaseous oxygen partial pressure in Torr e Dissolved oxygen partial pressure in ppm parts per million e Dissolved oxygen concentration in um L micromoles per liter e Data logging of temperature and oxygen readings in units listed above e In field single point reset also available on desktop
32. ardware Installation Hardware Installation Optical Fibers um 5 Temperature Probe USB Connector Analog Output Connector Power Connector WARNING Ensure that the plastic caps are covering the two SMA connectors on the front of the NeoFox unit Intense UV radiation is emitted from the LEDS when the unit is powered up Do NOT look directly at the LED output with the naked eye 8 013 20000 009 02 201510 Ocean hd ESE OO yoo gt Procedure Note You must install the NeoFox Viewer operating software prior to connecting the NeoFox hardware to the computer The NeoFox Viewer software installs the drivers required for the NeoFox hardware See Software Installation 1 Unpack the equipment and verify that you have all the necessary components see Shipment Components and Additional Recommended Equipment 2 Connect power cord from the power supply that came with your NeoFox unit from back of unit to an AC outlet Do NOT look directly at the light being emitted with the naked eye Caution Use only the power supply that came with your NeoFox unit Using a different power supply could damage your equipment Note On the NeoFox Sport hardware there is a difference between the power connector on the DPU module and the power connector on the NeoFox module The external power connector on the NeoFox module is the auxiliary power connector It provides power only to the NeoFox module
33. ata in an easy to use Microsoft Excel format NeoFox can be configured with single channel LED excitation and detection and modulation frequencies to 100 kHz The on board pressure transducer measures atmospheric pressure Software The NeoFox standalone module uses NeoFox Viewer software to display oxygen measurements on a connected Windows XP PC The NeoFox Sport can also use NeoFox Viewer software when connected to a Windows XP PC or it can be untethered from a computer and display some Oxygen measurements on its OLED screen See Chapter 4 Using NeoFox Sport for more information on the functionality of the NeoFox Sport Applications Typical applications include the following e Bioprocess monitoring e Environmental applications such as waste water management e Life science e Modified Atmosphere Packaging MAP Shipment Components NeoFox The following equipment and information ships with the standalone NeoFox O NeoFox Unit O SV Power Adapter Q Mini USB Cable O NeoFox CD This CD contains this manual and the NeoFox Viewer software 013 20000 009 02 201510 3 1 Introduction u Ocean Sem Packing List The packing list is inside a plastic bag attached to the outside of the shipment box the invoice arrives separately It lists all items in the order the shipping and billing addresses and any items on back order NeoFox Sport The following equipment and information ships with the NeoFox Sport
34. cify the name of the file where the settings will be saved Restoring a Chart Layout gt Procedure 1 Select View Restore Graph Layout from the menu 2 Enter the name of the file containing the saved setting s Saving Settings gt Procedure To save these values in the NeoFox Viewer 1 Navigate to the Advanced Settings screen 2 Set the Flash Write parameter to Enabled It will remain displayed as Enabled briefly before reverting to Disabled 013 20000 009 02 201510 31 Ocean Sem 3 To save these settings through the NeoFox Sport select Save Settings from the O2 Setup Menu 3 Using NeoFox with NeoFox Viewer Software Note When the device saves a parameter through a flash write it must save ALL of its savable settings at the same time Therefore actions which may appear to save specific settings to memory will actually save the entire set of savable parameters Those actions include downloading calibration data performing a single point reset or manually prompting a flash write by setting the Flashed Write parameter to Enabled in the Advanced Settings Panel Resetting Saved Settings You can reset the saved settings to their original state gt Procedure 1 Open the device in the NeoFox Viewer and navigate to the Advanced Settings screen 2 Set the value of the Flash Reset parameter to Enabled This value may revert to disabled shortly thereafter When the device is reset the saved values revert t
35. d phase cal BLUE Raw Phase 18 560 0 324 Most recent phase BLUE Sig Quality 92 127 dB 40397 5 Waveform Quality BLUE Amplitude 7361 26335 33696 Average waveform BLUE Peak 8749 25988 34737 Peak values hi and BLUE Phase StdDev 4 59 Phase standard dev RED Decay Time o Waveform decay ti RED Phase 16 624 0 290 Averaged phase cal 7 Setup Status Advanced Status Advanced Settings Advanced Settings This tab displays the advanced settings shown below for sensor waveform decay waveform FFT and salinity correction 20 013 20000 009 02 201510 3 Ocean kaf S Optics S NeoFox Viewer File View Options Window O Ocean Optics NeoFox NF_QMS24 Trends Oxygen ste Clear All V Data Oxygen 228 Setting Salinity Correction Temperature C Sensor Waveform Blue Red Both Sensor Data Red LED milliseconds v 0 LED Cycle Timing avgd O Using NeoFox with NeoFox Viewer Software 0 1 J 25us sensor Waveform Decay Waveform FFT Advanced Settings B Algorithm Settings LED Avg Cycles Phase Avg Count Blue Baseline Phase rad LED Recovery Cycles Disable Red LED Phase Avg Count Red Oxygen Units E Analog Output Voltage Output Source Current Output Source Voltage Minimum Limit Voltage Maximum Limit Current Minimum Limit Current Maximum Limit E Analog Settings
36. hold settings can be saved by the user to allow a NeoFox unit to operate in a stand alone mode outputting analog signals without input from a computer Configuring Analog Voltage Output Channel with NeoFox Viewer gt Procedure 1 Select the Advanced Settings tab on the Advanced View screen to view the advanced settings 013 20000 009 02 201510 47 JOce eee R PES A NeoFox Viewer File Options Window Enter New Event n Optics NeoFox NF OMS41 x Phase degrees Signal Blue red foihl avgd Sensor Data Red LED LED Cycle Timing 0 10us Sensor Waveform FFT Decay Waveform 7 Z ROXY gt FOSPOR APD Voltage ts ei v Advanced Settings Tab Bini 2e LED Rate KHz F Setup Status Advanced status Advanced Settings 2 In the Advanced Settings tab select an appropriate data source from the dropdown box located to the right of the Voltage Output Source setting For more info on valid sources please see Selecting a Data Source 3 Select a valid lower bound for your represented data range by typing a number into the box located to the right of the Analog Voltage Lower Bound setting In some versions of the Viewer software this setting may be called Voltage Limit Minimum For more information on selecting a lower bound for your voltage see Selecting a Data Range 4 Select a valid upper bound for your represented data range by
37. ibration one being the 0 oxygen concentration 013 20000 009 02 201510 Ocean dy Optics 3 Using NeoFox with NeoFox Viewer Software O NeoFox Calibration Two Point Oxygen Calibration 02 So Tau usec 2 143 Use Current Tau Computed Constants for Point 1 1 000 m b 2 20 900 1 450 MEE EE is 43 69550 43 69550 Tau Normalized Decay Time 10 11 O2 Concentrations Save Tau Calibration KEES g Download ei OK 7 Expose your oxygen sensor to 0 oxygen and let the Tau value stabilize You can observe the Tau value on the bottom of the screen to determine when it stabilizes 8 Click on the Use Current Tau for Point 1 box once the tau value is stable 9 Expose your oxygen sensor to the second known concentration of oxygen You can expose the probe to ambient conditions in air which is 20 9 oxygen 10 Enter the known oxygen value in the 02 box for point 2 11 Once the Tau value is stable click on the Use Current Tau for Point 2 box 12 Now that you have the two points of calibration click anywhere in the graph chart displayed on this screen The two points will appear on the graph circled in red on the figure above which will Compute Constants m and b y y S Download 13 To use this calibration for oxygen measurement click the button A Download Confirmation screen appears 14 Click Yes to confirm the downl
38. ith their equipment What s New in this Document This version of the NeoFox Installation and Operation Manual updates the operation instructions Document Summary Chapter Description Chapter 1 Introduction Contains descriptive information about the NeoFox It also provides a list of system requirements interface options and shipment components Chapter 2 nstallation Provides installation instructions Chapter 3 Using NeoFox Contains steps to operate NeoFox Viewer software with the NeoFox hardware Also contains steps for restoring screen defaults Chapter 4 Using NeoFox Sport Contains the menu structure and operational information for the NeoFox Sport Appendix A Specifications Lists NeoFox specifications Appendix B Maintenance Contains information for updating firmware and software and a troubleshooting table Appendix C Analog Output Provides information about NeoFox s analog output connector Product Related Documentation You can access documentation for Ocean Optics products by visiting our website at http www oceanoptics com Select Support Technical Documents then choose the appropriate document from the available drop down lists e Jaz Installation and Operation Manual e NeoFox Calibration Engineering Note 013 20000 009 02 201510 Ocean ka AboutThisManual OPTICS e NeoFox Communication Interfaces Engineering Note Safety a
39. l seconds b male Ch Standard v e oe 2 Click the Start Logging button 3 Select the desired location to save the file 4 Specify the logging interval 5 Choose the data columns to export either Standard or Extended wide range of data Standard recommended setting includes the following Time stamp to tenth of a second Pressure Temperature Tau 30 013 20000 009 02 201510 OE dy Or ICS 3 Using NeoFox with NeoFox Viewer Software e 02 e Converted O2 6 Click Save Settings and Close Window to save the data NeoFox Communication Interfaces You may wish to write programs to interface directly to the NeoFox without using the NeoFox Viewer There are a number of ways to do so Through the NeoFox DLL through the NeoFox RS232 port only NeoFox GT units with an F or later in the third digit of its serial number support RS232 communications or through custom USB drivers See the NeoFox Communication Interfaces Engineering Note for more information see Product Related Documentation Saving Restoring Chart Layouts NeoFox Viewer allows you to save and restore the layout of the displayed charts The following settings can be saved and then restored e X axis duration e Vertical height settings e Y axis scale settings e Type s of data being displayed on multiple plotting areas within the chart window Saving a Chart Layout gt Procedure 1 Select View Save Graph Layout from the menu 2 Spe
40. menu 34 menu description 34 saved settings 39 setup 36 NeoFox Viewer 3 17 new event entering 16 O oxygen sensor probes 2 4 p probe calibrating 23 oxygen sensor 2 4 temperature 4 product related documentation iv 60 Ocean bier SE R recommended equipment 4 RedEye 4 restore chart layout 31 S safety warnings i salinity correction 21 save chart layout 31 saved settings 28 NeoFox Sport 39 sensor oxygen 2 4 select type 22 setup NeoFox Sport 36 Setup tab 18 shipment components 3 software 3 5 17 NeoFox Viewer 3 updating 43 Software and Resources Library CD 4 specifications 41 Status tab 19 T tab Advanced Status 20 Setup 18 Status 19 troubleshooting 43 U updating 43 W what s new 1v 013 20000 009 02 201510
41. n Sensor Enables you to view the sensed values for Time O2 temperature and Tau The Readout values displayed refresh every time a new sample is uploaded from NeoFox with the appropriate values Prs Displays the atmospheric pressure measured by the on board pressure sensor on the NeoFox Sport O2 Displays the oxygen unit label and the current oxygen value Temp C Displays the algorithm temperature in degrees Celsius Tau us Displays the value of tau fluorescence lifetime Sensor Setup Allows you set the parameters shown below Single Pnt Reset Allows you to set the following parameter settings to be used when a single point reset is performed e Mole Default 20 90 Range 000 00 100 00 e Temperature Default algorithm temperature Range 000 00 200 00 C e SPR Pressure kPa Default On board pressure sensor e Tau Default current value of Tau Range 00 000 50 0000 A message displays informing you that the single point reset has completed NOTE NeoFox must be configured to Single Point mode through NeoFox Viewer Data Logging Allows you to enable datalogging to an SD card set the datalogging interval and see the filename used for the stored data Default current datalog interval Range 1000 99 999 999 ms Oxygen Units Enables you to set the units in which the O2 data is displayed Choose from PPO ppm torr and pmol L mole Temperature Allows
42. nd Compliance This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at his own expense WARNING The authority to operate this equipment is conditioned by the requirement that no modifications will be made to the equipment unless the changes or modifications are expressly approved by the manufacturer vi 013 20000 009 02 201510 Chapter 1 Introduction NeoFox System Overview The NeoFox is a device for measuring fluorescence lifetime phase and intensity It uses LED excitation and photodiode detection with filter based wavelength selection for easy experimental set up and control Because the unit is self contained it is invariant to fiber bending and stray light and has a wide dynamic range of optical intensity as well as low optical and electronic crosstalk and low drift and phase noise Therefore the NeoFox is especially useful for oxygen sensing applications where st
43. ng Stop Logging Close Window Without Saving Changes Standard GE LA When you click Start Logging samples are exported from that point onward and continue until you click Stop Logging and then click Save Settings and Close Window 16 013 20000 009 02 201510 Ocean Sem 3 Using NeoFox with NeoFox Viewer Software Note User defined events entered in the Enter New Event field on the basic NeoFox Viewer screen are not written to the data logging export file Field Description Start Logging Initiates data logging process Actual data logging does not start until you click Save Settings and Close Window Stop Logging Click this button to stop data logging Data logging does not actually stop until you click Save Settings and Close Window Specify Output File Click Choose to select the file to which the data will be exported If the file already exists you will be asked if you want to overwrite its contents Specify Logging Interval Select how frequently you want data records to be written to the log file Choose Columns to Export Select to export one of the following data types All Columns Exports all available types of data Standard Exports a limited subset of the available data types 02 Tau temperature pressure Close Window Without Saving Changes Closes this window None of the changes that you have made will take effect or be saved Save Setti
44. ng may be called Current Limit Minimum For more information on selecting a lower bound for your voltage see Selecting a Data Range 4 Select a valid upper bound for your represented data range by typing a number into the box located to the right of the Analog Current Upper Bound setting In some versions of the Viewer software this setting may be called Current Limit Maximum For more information on selecting a upper bound for your voltage see Selecting a Data Range You can save your settings by enabling the Flash Write setting momentarily Selecting a Data Source There are six potential data sources that can be driven by the NeoFox The pressure mode is not currently supported as an analog data source 013 20000 009 02 201510 49 OE C Analog Output dy Or Ics Mode Meaning 0 Off This mode does not actually turn the current source off Instead it sets the current source to 4 mA and the voltage source to 0 V It does not reduce the unit s power draw 1 Oxygen This is the standard percent oxygen mode The data source will be percent oxygen not a converted unit such as ppm or torr See Mode 5 for more information on these alternate units 2 Temperature Temperature in degrees Celsius 3 Pressure Not yet supported 4 Tau In microseconds 5 Alternate O Units In this mode the dimensionless value of the alternate O2 value is compared with the lower and upper bound pa
45. ngs and Close Window All changes that you have made on this window are saved If you have clicked Start Logging data logging will start the moment you click Save Settings and Close Window If you have clicked Stop Logging data logging will stop the moment you click Save Settings and Close Window Advanced Settings The NeoFox Viewer software provides a lot of information and features However most users will probably only use these functions e Oxygen reading Use the pick list above the Oxygen Reading to select to display oxygen as 0 P torr pO torr DO ppm or mg L or DO umol L e Sensor type selection FOXY HIOXY or FOSPOR e Calibration e Salinity Correction This screen is available from the View Advanced Settings menu 013 20000 009 02 201510 17 3 Using NeoFox with NeoFox Viewer Software dr Optics o NeoFox Viewer File View Options Window Enter New Event O Ocean Optics Neg Options Calibration Trends Sensor Waveform Oxygen Tau usec Signal Scans to Average Blue Red Bath Avgd Raw v Stop Sensor Data Red LED Data Export Interval Clear all Data milliseconds LED Cycle Timing 0 1 T Oxygen Reading Ss Sensor Waveform Decay Waveform FFT 22 8 22 5 22 2 i Setup 21 9 Probe Type Selection 21 6 Sensor Wrox 2 Hioxv TE FOSFOR 21 3 Gain Automatic Manual 21 0 0
46. o NeoFox Calibration Multi Point Calibration DEX 1 Open Or Start A New Calibration Open Calibration 2 Select Calibration Method None 02 results will not be calculated Two Point Calibration Simple calibration using two user entered O2 data points Multi Point Calibration Enter calibration data with many temperature OZ data points Single Point CalibratioiUser provided temperature OZ data point to recalibrate a multi point calibration 3 Select How Temperature Will Be Measured None Two point calibration only Use NeoFox Temperature Sensor Use 4 Fixed Temperature C 4 Enter Sensor Type And Optional Data 0 000 o FOXY Serial HIOXY FOSPOR Notes Date 4 1 2009 v 5 Enter Calibration Data In The Selected Tab 6 Save Calibration Data And Download To Board I Save J Calibration Tau Under Select Sensor Calibration Method choose Multi Point Calibration 4 Under Select How Temperature Is Measured choose Use NeoFox Temperature Sensor Under Enter Sensor Type And Optional Data choose the type of sensor and add the serial number and any notes needed Normalized decay time 10 t Note Open 6 Click the L Calibration NeoFox CD 7 Select the Multi Point button then browse to the location of the factory calibration file on the Calibration tab The uploaded calibration table appears on the left side of
47. o their factory settings 3 Download your calibrations again after resetting the flash memory Note For devices with firmware version 4 00 or above the APD Bias Voltage displayed as DAC APD Gain setting value persists through a flash reset 32 013 20000 009 02 201510 Chapter 4 Using NeoFox Sport Overview The NeoFox Sport is a field portable extension of the NeoFox oxygen measurement system Users of the NeoFox Sport have the ability to make real time measurements in the field without a computer or external power supply To do this the NeoFox Sport system uses the same NeoFox measurement hardware as the desktop NeoFox system but also includes a battery to supply power and a data processing unit to provide a user interface Because the NeoFox Sport uses the same measurement hardware as the standalone NeoFox user experience such as setup and measurement are common between both systems Both systems can be connected to a PC via USB and controlled through the NeoFox Viewer software In fact the user interface for the desktop NeoFox and the NeoFox Sport are exactly the same This chapter contains information for using NeoFox Sport without the NeoFox Viewer software NeoFox Sport Features Additional features that are available to NeoFox Sport users include e Battery powered operation e Real time display of temperature and oxygen in various measurement units e Gaseous oxygen partial pressure as a percentage of 1 AT
48. oad and continue The main NeoFox Viewer window will now display oxygen measurements 013 20000 009 02 201510 27 3 Using NeoFox with NeoFox Viewer Software t Optic g NeoFox Calibration Two Point Oxygen Calibration 02 90 Tau usec 1 0 000 2 143 Use Current Tau for Point 1 m b 2 20900 1 450 Gee ren Lau 43 69550 43 69550 Tau Normalized Decay Time 1 2 3 4 5 6 7 8 9 10 11 12 OZ Concentrations Save Tau Calibration EEN IECELT Download A OK p Note Normalized decay time 10 1 Saved Settings The following parameters are saved onboard the NeoFox in its non volatile memory When the settings are saved the saved values will be loaded when the device turns on Measurement Parameters e Duty Cycle Seconds on e Duty Cycle Seconds off e Number of Averages e Oxygen Units e Salinity Correction Factor 28 013 20000 009 02 201510 Ocean SES ics Calibration Settings Temperature Settings e Temperature Source e Fixed Temperature Value Calibration Method Sensor Type Two Point Calibration Settings e Two Point Tau0 e Two Point Taul e Two Point Oxygen 0 e Two Point Oxygen e Two Point Slope e Two Point Offset e Two Point Temperature Multipoint Calibration Settings 3 Using NeoFox with NeoFox Viewer Software e 12 Multipoint Original Calibration Coefficients AO Al A2 BO B1 B2 CO C1 C2 TO T1 T2
49. ocedure to install NeoFox Viewer software on a Windows XP PC The process on Windows Vista machines is very similar The important thing to note is to make sure to install the driver from the specific location within the NeoFox Viewer USB Driver directory Note You may be prompted to install NET runtime libraries if you do not already have them installed on your computer You can select to install them from the Internet or you can run the vc redist setup exe program located on your NeoFox installation CD However this installation program will also need to connect to the Internet to download the necessary files gt Procedure To install the NeoFox software 1 Close all other applications running on the PC 2 Start the software installation process 013 20000 009 02 201510 5 2 Installation Ocean DS Optics Installing from CD a b C Insert the CD containing the NeoFox Viewer software Select the drive on your computer with the software CD Double click on the NeoFox Viewer Setup msi installer program for Windows XP or the NeoFox Vista msi installer for Vista computers The installation wizard appears Installing from the Web d e Go to http www oceanoptics com technical softwaredownloads asp Right click on NeoFox Viewer Software and select Save Target As to download the executable to your machine Click Save The installation begins Click Run The NeoFox Viewer Setup screen appears
50. og output 4 20mA analog output RS232 is available on the NeoFox GT model with an F or greater in the third digit of its serial number NeoFox Sport Upload Interval The NeoFox Sport uploads data from the NeoFox module at least once per second in the Sport configuration Alternate configurations may have lower data rates Battery Life In the NeoFox Sport configuration a fully charged gently used battery will last for approximately three hours 013 20000 009 02 201510 41 Ocean ASpeciicatons OPTICS 42 013 20000 009 02 201510 Appendix B Maintenance Updating Firmware Do not unplug the device while the firmware is updating Caution Do not download a firmware version that predates the version you received with your NeoFox unit gt Procedure 1 Download the new NeoFox firmware image from www oceanoptics com technical softwaredo wnloads asp 2 Run Neofox Viewer and select OptionslFirmware Update 3 In the firmware update dialog select Download then choose the file that you just downloaded Updating Software You can overwrite the previous installation gt Procedure 1 Download the installer for the latest version of the Neofox Viewer from the Ocean Optics Software Downloads page www oceanoptics com technical softwaredownloads asp 2 Unzip the directory and then run the Setup msi installer wizard Troubleshooting Problem Possible Cause Suggested Solu
51. ound resulting in 4 mA 50 013 20000 009 02 201510 Ocean Sem Analog Data Range Example C Analog Output As an example consider the following A user who is interested in using the analog voltage output to monitor oxygen in the range from 4 5 ppm to 7 ppm would select the following settings The result would be that any ppm values below 4 5 ppm would result in an output of 0 volts and any values above 7 ppm would result in 5 volts In this case 6 3 ppm would produce an analog voltage output of 6 3 4 5 7 4 5 5 3 6 volts e Analog Voltage Source 5 Alternate O2 Units e Analog Voltage Lower Bound 4 5 e Analog Voltage Upper Bound 7 00 e Oxygen Units DO ppm Water Selection of ppm as alternate O2 units Saving the Analog Output Settings To save the analog output settings open the Advanced Settings control panel and then select Enabled from the dropdown box located to the right of the Flash Write setting This box will momentarily continue to display enabled but will switch back to disabled when the unit has processed the command Analog Output Pinouts Caution Be careful when wiring Incorrect wiring could cause permanent damage to the unit 4 3 1 RS232 Rx 7 OON 2 0 5V V k A 3 Current 4 20 V Tout to o 4 Current 4 20 V 6x 2 4 5 RS232 Tx Em 6 0 5V RS232 GND View of Connector on Back of NeoFox Unit
52. pdated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search for software O Yes this time only O Yes now and every time connect a device Click Next to continue gt Procedure 1 From the first hardware wizard screen select No not this time Click Next The next hardware wizard screen appears 12 013 20000 009 02 201510 2Ocean des ME ren 2 Select Install the software Automatically Recommended Click Next The system installs the necessary driver software to operate your NeoFox device The next hardware wizard screen appears 3 When the hardware driver installation completes successfully the following screen appears Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for Ocean Optics NeoFox Click Finish to close the wizard 013 20000 009 02 201510 13 Ocean Sem 2 Installation 14 013 20000 009 02 201510 Chapter 3 Using NeoFox with NeoFox Viewer Software Overview When you first display the NeoFox Viewer software the basic NeoFox Viewer screen appears File View Options Window Help Enter New Event O Ocean Optics NeoFox NF OMS24 x Trends Scans to Average Sensor Selection me Stop Gain Selection Gain
53. pect to any loss or damages arising from the information contained in this manual Important Safety Notices WARNINGS 1 Intense UV radiation is emitted from the NeoFox LED Do not look directly at the LED output with the naked eye once the SMA cap is removed To verify there is power to the instrument place a white piece of paper in front of LED 1 2 Do not open the instrument case High voltage is present There are no serviceable parts inside Return the instrument to the factory for service 013 20000 009 02 201510 Table of Contents About This RE e RE SR Re ES a di a a LOM V Document Purpose and Intended Audience mm ear v What s New in this Document aaa v Document SUMMANY EE v Product Related DocumentatiON aa v Safety and Compliance EE vi Chapter 1 Introduction sesinin ER RR a EE ER ER Ee EER esli 1 NeoFox System OV TVIEW ees Ee Eege eeh Ee dee 1 NGOFOXs AE N EE EE a OE EE eed 1 NeoEoSDOR nam GE RE END CR TO i de ee ae ee eee 2 au Zu IE N ON OR EE IE EE ee 2 Oxygen Sensor ProDeS EE EE een Mi aya eek ees ER GEWER NEEN Ee ENEE de EN 2 E AO EA ED N EO EE OE EO EN ER 3 aleet 3 ShipmentGormponerls EE 3 INCOR OX ee ee ee M ee sia ass Ee 3 Neolox EE 4 Additional Recommended Eguipment aaaa asas 4 Chapter 2 installatlon aaa EE EE EE 5 BEE e AR a OE EA DR ER 5 S ftware Installation EE 5 Hardware Installation eee eeeeeee erre aeee ee ee ee ee ee
54. ppears Select I don t have the disc Show me other options The next hardware wizard screen appears 4 Select Browse my computer for driver software advanced The next hardware wizard screen appears 5 Highlight the driver_support directory located beneath C Program Files Ocean Optics NeoFox Viewer and click OK followed by Next The next hardware wizard screen appears 6 If the following screen appears select Install this driver anyway Don t install this driver software You should check your manufacturer s website for updated driver software for your device i gt Install this driver software anyway Only install driver software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal information i V See details 7 When the hardware driver installation completes successfully the following screen appears 013 20000 009 02 201510 11 gt Ocean kw Uu Found New Hardware Ocean Optics NeoFox The software for this device has been successfully installed Windows has finished installing the driver software for this device Ocean Optics NeoFox Close Installing Hardware Drivers Using Windows XP The following Found New Hardware wizard appears when you first attach NeoFox to computers running the XP operating system Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and u
55. rameters Selecting a Data Range The data ranges of the analog outputs are fully adjustable through the lower bound and upper bound settings in NeoFox Viewer These settings are simply unitless values that are compared to the unitless value of the data source Analog Voltage Boundaries The analog voltage lower bound represents the value of the data source that corresponds to a O volt analog output The analog voltage upper bound represents the value of the data source that corresponds to a 5 volt analog output Intermediate values are linearly scaled across this region to produce the appropriate output Values of the data source that fall outside this region result in output voltages that are either 0 volts or 5 volts depending on whether the value of the data source exceeds the upper bound resulting in 5 V or falls short of the lower bound resulting in OV Analog Current Boundaries The analog current lower bound represents the value of the data source that corresponds to a 4 mA analog output The analog current upper bound represents the value of the data source that corresponds to a 20 mA analog current output Intermediate values are linearly scaled across this region to produce the appropriate output Values of the data source that fall outside this region result in output currents that are either 4mA or 20 mA depending on whether the value of the data source exceeds the upper bound resulting in 20 mA or falls short of the lower b
56. re 4 20 mA Isolation Some users especially those in industrial settings may need to use an isolated 4 20 mA loop Unfortunately the current sink on the NeoFox unit is not isolated and is not suitable for use in environments which may cause the loop voltage to exceed 30V However there are a number of third party products available that can convert the output of the NeoFox into an isolated 4 20 mA loop transmitter C Analog Output Some suitable products include e LPI 1 e Manufacturer A Pi http www api usa com e Suggested Vendor Mod tronic http www mod tronic com e Part Number LPI 1 e 931S A1AIN IP1 e Manufacturer Rockwell Automation e Suggested Vendor Varies by geographic region e Part Number 931S A1A1N IP1 e 931S C1A2D OP e Manufacturer Rockwell Automation e Suggested Vendor Varies by geographic region e Part Number 931S C1A2D OP This part can be used to convert the 0 5V output of the NeoFox into a 4 20mA signal Resistor Volt Meter If an ammeter is not available as is the case with many data acquisition systems a voltmeter can be used instead see following figure To use a voltage measurement system users should select a low impedance resistor and a high impedance voltage measurement instrument Be aware that there will be a voltage drop across the resistor that substracts from the loop voltage supply The voltage drop can be calculated as resistance times current For example a 100 Ohm
57. refore be sure to pass only initialized strings Better error handling for this error was added in DLL version 1 89 but you should still use this convention to avoid unnecessary complications After setting the display units to anything other than ppm the display will not return to percent oxygen Previous software version This problem was resolved in version 1 92 of the Neofox Viewer Contact Ocean Optics if you see this error in a more recent software version Previously this problem was solved by reinstalling the NeoFox Viewer software Percent oxygen is above 100 or below 0 Sensor probe has been calibrated incorrectly Recalibrate the sensor probe A fatal error or critical error warning message appears at startup and prompts to continue or exit the application After continuing the FFT view does not seem to work This problem seems to occur when the application starts with the FFT View Window active Select Continue then select the waveform or decay form view Close the application and then restart The problem should not occur again If the problem persists reinstall the NeoFox Viewer software 44 013 20000 009 02 201510 Ocean SES ics B Maintenance Problem Possible Cause Suggested Solution The HIOXY automatic frequency setting defaults to 45 KHz instead of 100 KHz HIOXY calibrations performed prior to 31 AUG 2009 yield incorrect results on
58. tion Clicking Download during a The Two Point Calibration Follow the Two Point Calibration Two Point Calibration does not was not performed correctly instructions in this manual exactly as change the measured values of written After scanning the tau values a single click on the graph area was not performed oxygen 013 20000 009 02 201510 43 B Maintenance Ocean RIER Problem Possible Cause Suggested Solution At startup the device s controls appear disabled no values display or the device shows other signs of not uploading data The NeoFox drivers and firmware versions are incompatible Update the NeoFox firmware and NeoFox Viewer software See Updating Software for more information During hardware installation the Found New Hardware Wizard can t find the driver or sys file for the NeoFox The NeoFox directory isn t included in search When prompted choose to install from a specific location then include the NeoFox installation directory in your search This directory is located at C Program Files Ocean Optics Neofox Viewer by default The drivers can be found in the USB Drivers directory located here After running a VI that calls the Neofox DLL LabView crashes when that VI is closed The VI passes a function in the library an uninitialized string The library functions generally assume that any strings passed are already initialized The
59. typing a number into the box located to the right of the Analog Voltage Upper Bound setting In some versions of the Viewer software this setting may be called Voltage Limit Maximum For more information on selecting an upper bound for your voltage see Selecting a Data Range You can save your settings by enabling the Flash Write setting momentarily Configuring the Analog Current Output Channel with NeoFox Viewer gt Procedure 1 Select the Advanced Settings tab on the Advanced View screen to view the advanced settings 48 013 20000 009 02 201510 a S an Optics ol NeoFox Viewer C Analog Output File Options Window Enter New Event O Ocean Optics NeoFox NF_QMS41 Trends Sto J E Sensor Data Red LED Clear all Y Kee fe Ed LED Cycle Timing DO MEE e 10us Sensor Waveform FFT Decay Waveform Setup Sensor a FOXY Gain Automatic Manual Advanced Settings Tab 15 sec 20 sec Setup Status Advanced Status uladvanced Settings 2 Select an appropriate data source from the dropdown box located to the right of the Current Output Source setting For more info on valid sources see Selecting a Data Source 3 Selecta validlower bound for your represented data range by typing a number into the box located to the right of the Analog Current Lower Bound setting In some versions of the Viewer software this setti
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