Agilent 1200 Series Manual Injector User Manual
Contents
1. Leak channel Figure 8 Leak Drainage 1200 Series MI User Manual 15 2 Installing the Manual Injector Leak Drainage 16 1200 Series MI User Manual 1200 Series MI User Manual 3 Using the Manual Injector Solvent Information 18 Choice of Injection Seal 19 Needles 20 Injecting Sample 21 ee Agilent Technologies 3 Using the Manual Injector Solvent Information 18 Observe the following recommendations on the use of solvents Flow Cell Long term operation at pH gt 11 should be avoided Never leave strongly alkaline solutions in the flow cell without flow Solvents Always filter solvents through 0 4 um filters small particles can permanently block filters frits and capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCI13 02 2COCI2 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers2. Cleaning the Manual Injector The manual injector base should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent 26 1200 Series MI User Manual Maintenance Stator Face When Poor injection volume reproducibility Leaking injection valve Tools required Hex key 9 64 inch supplied with valve Parts required Part number Description 1 0100 1859 Stator face 1 Loosen the three stator screws 2 Remove the stator head and st ator face 1200 Series MI User Manual 27 4 Maintenance 3 Insert the new stator face onto the stator head stator head is secure e gt Pan 7 4 Install the stator head and stator face onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head 5 Secure the stator face and stator head in place with the stator screws Tighten each screw alternately turn until the 28 1200 Series MI User Manual Injection Valve Seal When 5 Leaking injection valve Maintenance Poor injection volume reproducibility Tools required Hex key 9 64 inch supplied with valve Parts required Part number Description 1 0100 0623 Rotor seal Vespel 0100 0620 Tefz el 4 1 Loosen the three stator screws 2 Remove the stator head and stator face see Stator Face on page 27
3. which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol may attack steel Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel 1200 Series MI User Manual Using the Manual Injector 3 Choice of Injection Seal The manual injector is supplied with a Vespel injection seal as standard Vespel is sensitive to alkaline attack so avoid mobile phases with a pH of 10 or more Use the Tefzel injection seal see Injection Valve Assembly on page 38 for mobile phases with a pH above 10 1200 Series MI User Manual 19 3 Using the Manual Injector Needles CAUTION Needle can damage valve gt Always use the correct size needle The manual injector is not supplied with syringes or needles Use needles with 0 028 inch outer diameter 22 gauge x 2 inch long needle without electro taper and with 90 point style square tip 20 1200 Series MI User Manual Using the Manual Injector 3 Injecting Sample WARNING Ejection of mobile phase When using sample loops larger than 100 pl mobile phase may be ejected from the needle port as the mobile phase in the sample loop decompresses gt Please observe appropriate safety procedures for ex
4. 1200 Series MI User Manual 29 4 Maintenance 3 Remove the stator ring 4 Remove the seal 5 Install the new seal Ensure the seal is positioned as 6 Install the stator ring Ensure the pin in the stator ring is shown aligned with the hole in the valve body Rotor pin Stator ring Notch Needle hole 30 1200 Series MI User Manual Maintenance 4 7 Insert the stator face onto the stator head 8 Install the stator head and stator face onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head 9 Secure the stator face and stator head in place with the stator screws Tighten each screw alternately 4 turn until the stator head is secure SS A ee 1200 Series MI User Manual 31 4 Maintenance Position Sensing Switch When No start signal when switching to the inject position Tools required Hex key 9 64 inch supplied with valve Parts required Part number Description 1 0490 1849 Position sensing switch 1 Loosen the three stator screws 2 Remove the stator head and stator face see Stator Face on page 27 32 1200 Series MI User Manual Maintenance 4 Position Sensing Switch 3 Remove the stator ring 4 Disconnect the sensor cable from the start cable Pull the sensing switch out of the stator ring 5 6 Install the stator ring Ensure the pin in the stator r
5. 2 Connecting the Organizer Plates 3 Screw the mounting pole into one of the two holes in the organizer plate 1200 Series MI User Manual 9 2 Installing the Manual Injector 4 Slide the manual injector onto the mounting pole see Figure 3 on page 10 Tighten the mounting screw Figure 3 Installing the Mounting Pole and Manual Injector 10 1200 Series MI User Manual Installing the Manual Injector 2 5 Install other system modules on top of the manual injector baseplate see Figure 4 on page 11 E T L Figure 4 Installing the System 1200 Series MI User Manual 11 2 Installing the Manual Injector the Vianua yector APG remote connector Start cable Position sensor cable gogog agaaa EEA c EEEE gt K 2332222 Tea a oo00000 See 1 F Figure 5 Installing the Start Cable 1 See Figure 6 on page 14 6 Connect the capillaries to the manual injector see Flow Connections on page 13 12 1200 Series MI User Manual Installing the Manual Injector 2 Flow Connections WARNING Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary or tube fittings sol
6. Agilent Technologies on Internet 42 Agilent Technologies 41 6 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the internet at http www chem agilent com 42 1200 Series MI User Manual Index Index A Agilent on internet 42 APG remote 12 B buffers 25 C capillary connections 13 cleaning the manual injector 26 D damaged packaging 8 delivery checklist 8 description 6 E exchanging injection seal 29 position sensing switch 32 stator face 27 F flow cell solvent information 18 flow connections 13 flushing the manual injector 25 information oninternet 42 INJECT 6 22 1200 Series MI User Manual injecting sample 6 injection seal 29 tefzel 19 vespel 19 installation 9 installing the manual injector 9 internet 42 42 L leak channel 15 leak drainage 15 leaks 13 LOAD 6 21 M make before break 6 needle type 20 needle port cleaner 25 needles 20 P position sensing switch 32 position sensor cable 12 precision 21 repair procedures injection seal 29 position sensing switch 32 stator face 27 Rheodyne 77251 6 S salt solutions 25 sample loops 6 sample volume 21 solvents acids 18 buffers 25 ethers 18 rinsing 25 salt solutions 25 steel corrosive 18 start cable 12 stator face 27 U unpacking damaged packaging 8 delivery checklist 8 Ww worldwide web 42 43 ww
7. 2 Overview of Repair Procedures Procedure Typical Frequency Time Required Notes Flushing the injector After using aqueous buffers or 5 minutes See Flushing the Manual Injector on page 25 salt solutions Exchanging the When visibly scratched or 10 minutes See Stator Face on page 27 stator face when the valve performance shows indication of leakage or wear Exchanging the After approximately 10000 to 10 minutes See Injection Valve Seal on page 29 injection valve seal 20000 injections or when the valve performance shows indication of leakage or wear Exchanging the When cable damaged or when 10 minutes See Position Sensing Switch on page 32 position sensing no start signal is sent when switch switching to the inject position 24 1200 Series MI User Manual Maintenance 4 Flushing the Manual Injector CAUTION Damage through crystal formation The use of aqueous buffers or salt solutions can lead to crystal formation which may cause scratches on the injection seal gt Always rinse the valve with water after using aqueous buffers or salt solutions 1 Switch the valve to the INJECT position 2 Use the pump to flush the sample loop and seal grooves 3 Use the needle port cleaner supplied with the valve and syringe to flush the needle port and vent capillary Needle port Syringe Needle port cleaner Figure 11 Needle port Cleaner 1200 Series MI User Manual 25 4 Maintenance
8. Agilent 1200 Series Manual Injector User Manual ot Agilent Technologies Notices Agilent Technologies Inc 2006 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61328 90011 Edition 11 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the s
9. ample goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Needle port Waste to column Sample loop LOAD Position In the LOAD position see Figure 9 on page 21 the pump is connected directly to the column ports 2 and 3 connected and the needle port is connected to the sample loop At least 2 to 3 sample loop volumes more if better precision is required of sample should be injected through the needle port to provide good precision The sample fills the loop and excess sample is expelled through the vent tube connected to port 6 from pump Figure 9 LOAD Position 1200 Series MI User Manual 21 3 Using the Manual Injector INJECT Position In the INJECT position see Figure 10 on page 22 the pump is connected to the sample loop ports 1 and 2 connected All of the sample is washed out of the loop onto the column The needle port is connected to the vent tube port 5 Needle port to column from pump Sample loop Figure 10 INJECT Position 22 1200 Series MI User Manual 1200 Series MI User Manual 4 Maintenance Overview of Maintenance 24 Flushing the Manual Injector 25 Cleaning the Manual Injector 26 Stator Face 27 Injection Valve Seal 29 Position Sensing Switch 32 a Agilent Technologies 23 4 Maintenance Overview of Maintenance Table
10. d into the external 20 ul sample loop through the injection port at the front of the valve The valve has a ceramic stator and Vespel injection seal for pH above 10 a Tefzel seal is available A make before break passage in the stator ensures flow is not interrupted when the valve is switched between the INJECT and LOAD positions and back again see also Needles on page 20 and Flow Connections on page 13 The valve is mounted on a steel mounting pole and can be installed at the left or right hand side of the LC system Mounting pole Injection port Figure 1 Rheodyne 7725i Injection Valve 6 1200 Series MI User Manual 1200 Series MI User Manual 2 Installing the Manual Injector Unpacking the Manual Injector 8 Damaged Packaging 8 Delivery Checklist 8 Installing the Manual Injector 9 Flow Connections 13 Leak Drainage 15 gy Agilent Technologies 2 Installing the Manual Injector Unpacking the Manual Injector Damaged Packaging Upon receipt of your manual injector inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the manual injector has been mechanically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carriers inspection Delivery Checklist Ensure all parts and materials have been del
11. ep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully unde
12. ing is aligned with the hole in the valve body ey 6 1200 Series MI User Manual 33 4 Maintenance Position Sensing Switch 7 Insert the stator face onto the stator head 8 Install the stator head and stator face onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head 9 Secure the stator face and stator head in place with the stator screws Tighten each screw alternately 4 turn until the stator head is secure i i O 34 1200 Series MI User Manual 1200 Series MI User Manual pm and Materials for Maintenance Manual Injector 36 Injection Valve Assembly 38 phe Agilent Technologies 35 5 Parts and Materials for Maintenance Manual Injector Table 3 Manual Injector Item Description Part Number 1 Manual injection valve see Injection Valve Assembly on 5063 6502 page 38 2 Mounting pole 5001 3738 3 Base plate G1328 44111 4 Organizer plate 5042 8553 Catch tube cap 5042 8576 5 Name plate for Agilent 1200 Series 5042 8901 Valve syringe fixed needle 50 ul 5182 9619 Connection capillary 0 17 mm id 500 mm not shown G1328 87600 Start cable not shown 0100 1677 36 1200 Series MI User Manual Parts and Materials for Maintenance 5 Manual Injector Figure 12 Manual Injector 1200 Series MI User Manual 37 5 Parts and Materials for Maintenance Injection Valve A
13. ivered with the manual injector The delivery checklist is shown in Table 1 on page 8 To aid in parts identification please see Parts and Materials for Maintenance on page 35 Please report missing or damaged parts to your local Agilent Technologies sales and service office Table 1 Manual Injector Checklist Description Quantity Part Number Manual injection valve with start cable including 1 5063 6502 operating instructions needle port cleaner vent tubes x2 and fittings 5 64 and 9 64 inch hex keys Mounting pole 1 5001 3738 Connection capillary 0 17 mm id 500 mm 1 G1328 87600 Base plate 1 G1328 44111 Organizer plate 1 5042 8553 Catch tube cap 1 5042 8576 Valve syringe fixed needle 50 ul 1 5182 9619 User Manual 1 G1328 90011 1200 Series MI User Manual Installing the Manual Injector 2 Installing the Manual Injector CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions The manual injector can be installed at the left or right hand side of the instrument stack 1 Place the baseplate on the bench 2 Connect the two organizer plates to the base plate Figure
14. rstood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1200 Series MI User Manual Contents Contents 1 Introduction 5 Introduction to the Manual Injector 6 2 Installing the Manual Injector 7 Unpacking the Manual Injector 8 Installing the Manual Injector 9 Flow Connections 13 Leak Drainage 15 3 Using the Manual Injector 17 Solvent Information 18 Choice of Injection Seal 19 Needles 20 Injecting Sample 21 4 Maintenance 23 Overview of Maintenance 24 Flushing the Manual Injector 25 Cleaning the Manual Injector 26 Stator Face 27 Injection Valve Seal 29 Position Sensing Switch 32 5 Parts and Materials for Maintenance 35 Manual Injector 36 Injection Valve Assembly 38 6 Appendix 41 Agilent Technologies on Internet 42 1200 Series MI User Manual Contents 4 1200 Series MI User Manual 1200 Series MI User Manual 1 Introduction Introduction to the Manual Injector 6 a Agilent Technologies 1 Introduction Introduction to the Manual Injector See also the Rheodyne 7725i operating instructions supplied with the injection valve The Agilent 1200 Series manual injector uses a Rheodyne 7725i 7 port sample injection valve Sample is loade
15. ssembly 38 Table 4 Injection Valve Assembly Item Description Part Number Manual injection valve with starts cable complete assembly 5063 6502 including operating instructions needle port cleaner vent tubes x2 and fittings 5 64 and 9 64 inch hex keys Includes items 1 8 Bearing ring order rebuild kit instead Isolation seal Rotor seal Vespel Rotor seal Tefzel Rotor Seal PEEK Stator face Stator head Stator screws Hex key 9 64 inch for stator screws not shown Position sensing switch not shown 0101 1254 0100 1857 0101 0623 0101 0620 0101 1255 0100 1859 0100 1860 1535 4857 8710 2394 0490 1849 1200 Series MI User Manual Parts and Materials for Maintenance 5 Injection Valve Ass Figure 13 _Injection Valve Assembly Sample loops Description Stainless Steel loops PEEK loops Sample loop 5 pl 0101 1248 0101 1241 Sample loop 10 pl 0100 1923 0100 1240 Sample loop 20 pl 0100 1922 0100 1239 Sample loop 50 pl 0100 1924 0100 1238 Sample loop 100 pl 0100 1921 0100 1242 Sample loop 200 pl 0101 1247 0101 1327 1200 Series MI User Manual 39 5 Parts and Materials for Maintenance Description Stainless Steel loops PEEK loops Sample loop 500 ul 0101 1246 0101 1236 Sample loop 1 ml 0101 1245 0101 1235 Sample loop 2 ml 0101 1244 0101 1234 Sample loop 5 ml 0101 1243 0101 1230 40 1200 Series MI User Manual 1200 Series MI User Manual enie
16. vents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used see also Leak Drainage on page 15 CAUTION Prevent siphoning gt The outlets of the two vent capillaries ports 5 and 6 and the needle port must be at the same level to prevent siphoning see Figure 7 on page 14 1 Connect the pump outlet capillary to port 2 2 Connect the column compartment inlet capillary to port 3 3 Connect the sample loop between ports 1 and 4 1200 Series MI User Manual 13 2 Installing the Manual Injector 4 Connect one vent capillary supplied with valve to port 5 and one to port 6 Sample loop Vent tube To column From pump outlet eN Figure 6 Flow Connections Figure 7 Vent Capillaries 14 1200 Series MI User Manual Installing the Manual Injector 2 Leak Drainage WARNING Leaking injector fittings In the event of a leak solvent will drop into the leak channel in the baseplate from where it is channelled to the front and back of the baseplate gt Check the manual injector fittings periodically for signs of leakage
17. w agilent com In This Book This manual contains user information about the Agilent 1200 Series manual injector The manual describes the following e introduction to the manual injector e installing the manual injector e using the manual injector e maintenance of the manual injector e parts and materials and e additional information Agilent Technologies 2006 2008 Printed in Germany 11 08 G1328 90011 fe Agilent Technologies
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