CytoBrite Slide Incubation System - User Manual
Contents
1. 19 XII ORDERING INFORMATION 20 XIII DECLARATION OF CONFORMITY 21 CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 3 408 733 7337 techserv scigene com I SAFETY NOTICES A Intended Use The CytoBrite Slide Incubation System is intended for the heating and cooling of biological samples The instrument should only be used according to instructions provided in this User Manual and other SciGene technical documents If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired La CytoBrite syst me est destin pour le chauffage et le refroidissement d chantillons biologiques L instrument ne devrait servir que selon les instructions fournies dans ce manuel de l utilisateur et d autres documents techniques de SciGene Si2. 7 D Environmental Requirements 7 E Installing the Instrument 7 III USING YOUR CYTOBRITE SYSTEM 8 A System Components 8 B Preparing Slides Loading the Instrument Starting a Protocol 9 C Powering On Off 9 D Using the Controller 9 E Using the USB Drive 9 IV CREATING AND EDITING PROTOCOLS 10 A Creating a Protoc
3. 17 408 733 7337 techserv scigene com Return to Sengs CytoBrite Run Create View Edit Files Settings SETTINGS 1 Block Type 2 Calibrate 3 Sounds 4 Upgrade SW SETTINGS Beep After 1 Protocol End Off 2 Each Select Off 3 Preheat Off 4 Denature Off SETTINGS Beep After Each Select etc On Off On SETTINGS Beep After 1 Each Select On 2 Each Step Off Sounds Changing Sounds C Upgrading Software The CytoBrite System displays the current software version on the controller when powering on the instrument during boot up New software versions are installed via the USB DRIVE Current software versions may be downloaded from www scigene com 1 From the Home screen go to Settings Upgrade SW 2 Following the on screen prompts insert a USB key containing new software into the USB DRIVE and press Select 3 A flashing progress indicator displays as the software is loading This process may take several minutes 4 When loading is finished an Upgrade Complete message will display Press any key to display the new software version number and date After a few seconds the Home screen
4. 408 733 7337 techserv scigene com X TROUBLESHOOTING Symptom Cause Solution Instrument does not turn on Blown fuse s Replace fuse s on back of unit beneath power cord receptacle Block is not reaching set temperature Paper or other object blocking fans Check underneath CytoBrite base for objects blocking the fans Ensure proper clearance XI SPECIFICATIONS Electrical Cat 2019 00 1 115 230V AC 50 60 Hz 3A 1 6A Dimensions cover closed Outside H x W x D 9 25 x 9 x 12 75 inches 24 x 23 x 32 cm Weight Instrument 15 lbs 7 kg net Performance Block Temperature Range 15 to 90 C Block Temperature Regulation 0 2 C from set point Block Temperature Accuracy 0 5 C Slide to slide Temperature Variation lt 1 0 C Heating Rate 37 to 75 C in lt 2 minutes Cooling Rate 75 to 37 C in lt 2 minutes Controller Controller Type Digital PID single loop Display Four line LED Calibration Output Thermocouple Environmental Ambient temperature Operation 15 to 32 C 58 to 90 F Storage 20 to 60 C 4 to 140 F Relative humidity
5. 15 A Datalogging to USB Key 15 B Calibrating Slide Block Temperature 16 C Upgrading Software 17 D Changing Alert Sounds 17 IX MAINTENANCE 18 A Cleaning the Instrument 18 B Cleaning Air Vents 18 C Checking and Replacing Fuses 18 X TROUBLESHOOTING 19 XI SPECIFICATIONS
6. 4 Wait 30 minutes 5 Calculate the difference between the temperature shown on the digital thermometer and the block temperature shown on the controller display to determine the needed offset adjustment For example if the thermometer reads 76 0 C and the controller reads 75 0 C the offset adjustment is 1 0 6 Add this adjustment value to the existing offset shown on the controller display to determine a new offset then enter the new value using the Forward and Back buttons For example if the offset value on the display before adjustment is 0 3 and the adjustment value from step 5 is 1 0 the new offset value to be entered into the controller would be 1 3 7 Select Yes to confirm the new offset and return to Home screen Calibrating Block Temp CytoBrite Run Create View Edit Files Settings SETTINGS 1 Datalog 2 Calibrate 3 Sounds 4 Upgrade SW Return to Home screen SETTINGS Calibrate Block Set Temp 75C SETTINGS Calibrate Block Set Temp 75C Yes No SETTINGS Block Temp 75 0 Offset 0 3 SETTINGS Calibration Complete at 75C Press any key SETTINGS Block Temp 75 0 OK Yes No CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015
7. 3rd Edition Part 1 General requirements RoHS Directive 2011 65 EU Terry Gill Sunnyvale California USA Name of Authorized Representative Place of Issue Director of Product Manufacturing March 19 2015 Title of Authorized Representative Date of Issue Signature of Authorized Representative
8. b perform factory repairs or c replace the product The foregoing warranty is void in the event the unit was abused or modified or used in a manner inconsistent with its intended purpose SciGene makes no other warranty expressed or implied including warranties of merchantability and fitness for a particular purpose In no event shall SciGene be liable for any direct indirect special incidental or consequential damagers or for any damages resulting from loss arising out of or in connection with the sale use or performance of the product Copyright Copyright 2014 2015 SciGene Corporation All rights reserved SciGene CytoBrite and CytoBond are trademarks of SciGene Corporation Sunnyvale CA All other trademarks used in this manual are the property of their respective owners CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 2 408 733 7337 techserv scigene com Table of Contents I SAFETY NOTICES 3 A Intended
9. and press Select 4 Select Yes to confirm CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 15 408 733 7337 techserv scigene com Creating a Datalog CytoBrite Run Create View Edit Files Settings SETTINGS 1 Datalog 2 Calibrate 3 Sounds 4 Upgrade SW Enter Datalog Frequency 5 sec 60 min 00 min 05 sec SETTINGS Datalogging On Turn Off Change Off If set to OFF If set to ON Datalog Frequency 00 min 05 sec Ok Yes No Return to Sengs Change SETTINGS Datalogging Off Turn On Yes No Yes VIII SETTINGS AND PREFERENCES A Datalogging to USB Key A text file that captures the block time and temperature while a protocol is running can be created and written to the USB key 1 From the Home screen go to Settings Datalog 2 Following the prompts on the screen turn on datalogging and enter the data sampling frequency 5 sec to 60 min 3 Insert a USB key into the USB DRIVE to capture a log file While in use the drive light will flash as data is transferred to the USB key A datalog
10. from a USB key Provide additional capacity for storing protocols beyond the 10 file storage limit on the controller Upload new controller software CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 10 408 733 7337 techserv scigene com Creating a Protocol CytoBrite Run Create View Edit Files Settings CREATE FISH101A Denature Yes No CREATE FISH101A Incubate Temp 37C CREATE FISH101A Hold Temp 37C CREATE FISH101A Denature Temp 73C CREATE FISH101A Protocol End Off Hold CREATE FISH101A Save in CytoBrite USB Return to Home screen CREATE Enter Name FISH101A No Yes Hold O CREATE FISH101A Denature Time 00 02 00 CREATE FISH101A Incubate Time 16 00 00 IV CREATING AND EDITING PROTOCOLS A Creating a Protocol Protocols are created and saved in memory on the controller 10 files maximum or a USB key Names are 8 alphanumeric characters in length including spaces For FISH ISH methods protocols can be created with an optional co denaturation step prior to probe hybridization 1 From the Home screen selec
11. the rear of the instrument indicates the presence of the fuse box Warning For Continued Protection Against Fire Replace Only with Same Type Rating of Fuse Always disconnect the power cord before attempting to replace the fuse Ce symbole sur l arri re de l instrument indique la pr sence de la bo te de fusibles Avertissement Pour le maintien de la Protection contre l incendie remplacer uniquement avec la m me cote de Type de fusible Toujours d brancher le cordon d alimentation avant d essayer de remplacer le fusible CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 5 408 733 7337 techserv scigene com E Cautions Failure to comply with the following cautionary statement affixed to the product may lead to possible personal injury Omission de se conformer la mise en garde suivante appos e sur le produit peut entra ner des blessures possibles This symbol located both on and in front of the lid indicates the potential presence of a Hot Surface Use care when working in this area to avoid being
12. visible damage If any damage is evident retain all shipping materials and contact SciGene techserv scigene com or your local distributor B Parts Provided 2x Slide Trays shipped inside instrument Power Cord User Manual C Coverslip Sealant Provided One complimentary 100 ml bottle of CytoBond Removable Coverslip Sealant SciGene cat 2020 00 1 is shipped separately from the instrument Use of CytoBond Sealant is required for sealing coverslips to prevent probe evaporation without humidification Contact SciGene custserv scigene com or your local distributor to order additional bottles D Environmental Requirements Ensure that the area where the CytoBrite System is installed meets the following conditions for reasons of safety and performance Ambient temperature 15 to 32 C 58 to 90 F Relative humidity 20 to 80 RH non condensing Air flow clearance 6 inches 15 cm minimum on both sides E Installing the Instrument Place the instrument on a level surface within a few feet of the power source Position the system so the lid can be easily opened without interference Allow 18 inches of vertical clearance and 16 inches of horizontal clearance Ensure 3 inches of clear
13. www scigene com 1287 Reamwood Avenue Sunnyvale CA 94089 USA Tel 408 733 7337 Fax 408 733 7336 techserv scigene com CytoBrite Slide Incubation System USER MANUAL Cat 2019 00 1 115 230V FOR RESEARCH USE ONLY CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 1 408 733 7337 techserv scigene com Serial Number The following serial number identifies the specific instrument you have purchased and must be referenced when requesting service A copy is affixed to the instrument Technical Service 408 733 7337 techserv scigene com Warranty SciGene warrants that the CytoBrite Slide Incubation System described in this manual shall be free of defects in materials and workmanship for a period of 12 months from date of delivery This warranty does not cover removable slide trays or accessories In the event of a defect during the warranty period SciGene s limit of liability will be to at its sole discretion a provide replacement parts
14. avec prudence This symbol indicates the OFF position of the main POWER switch Ce symbole indique la position OFF de l interrupteur principal This symbol indicates the ON position of the main POWER switch Ce symbole indique la position ON de l interrupteur principal 2 Non Electrical Symbols CAUTION This symbol illustrates a heat hazard Proceed with caution when working around these areas to avoid being burned by hot components ATTENTION Ce symbole illustre un danger pour la chaleur Faire preuve de prudence lorsque vous travaillez autour de ces zones pour viter d tre br l par les composants du chauds CAUTION This symbol alerts you to consult this Operator s Manual for further information and to proceed with caution ATTENTION Ce symbole vous avertit consulter ce guide de l utilisateur pour plus d informations et de proc der avec prudence D Warnings Failure to comply with the following warnings that are affixed to the product can lead to possible personal injury or death D faut de respecter les avertissements suivants qui sont appos es sur le produit peut conduire possibles l sions corporelles ou la mort This symbol on
15. burned Ce symbole situ sur et devant le couvercle indique la pr sence possible d une Surface chaude Faire preuve de diligence lorsqu il travaille dans ce domaine pour viter d tre br l This symbol located on the back of the instrument warns the user to select the correct voltage before connecting the power cord Operating with the voltage selector switch in the wrong position will damage the instrument and create a safety hazard Ce symbole situ l arri re de l appareil avertit l utilisateur de s lectionner la tension correcte avant de brancher le cordon d alimentation Fonctionnant avec le s lecteur de tension dans la mauvaise position peut endommager l appareil et cr er un risque de s curit F Compliance 1 European Conformity CE This symbol indicates the instrument is in compliance with all applicable European Union Electromagnetic Compatibility and Low Voltage Directives 2 Waste Electrical amp Electronic Equipment Directive WEEE This symbol indicates the instrument is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC providing environmentally safe disposal of end of life equipment through recycling Contact your institution
16. file for a typical protocol is shown below Datalog file for a typical protocol CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 16 408 733 7337 techserv scigene com B Calibrating Slide Block Temperature The CytoBrite System comes calibrated from the factory to provide accurate slide temperatures from 15 to 90 C 0 5 C The accuracy of block temperatures should be checked periodically according to your lab schedule with a T type NIST certified digital thermometer SciGene cat 1051 52 0 The controller needs calibration only if the block temperature shown on the NIST certified thermometer differs by more than one degree 1 C from the temperature shown on the controller display 1 Connect a T type NIST certified digital thermometer ScIGene cat 1051 52 0 to the blue jack on the back of the unit labeled Temperature Calibration 2 From the Home screen go to Settings Calibrate 3 Enter 75 C as set temperature and confirm The block temperature and current offset will be shown
17. l quipement est utilis de mani re non sp cifi e par le fabricant la protection assur e par l quipement peut tre compromise B Instrument Safety Before operating the instrument read the information in this section concerning hazards and potential hazards Ensure that anyone involved with the instrument s operation is instructed in both general safety practices for laboratories and specific safety practices for the instrument Avant le fonctionnement de l instrument lisez les renseignements dans cette section concernant les risques et les dangers potentiels S assurer que toute personne impliqu e avec le fonctionnement de l instrument est instruit dans les pratiques g n rales de s curit pour les laboratoires et les pratiques de s curit sp cifiques pour l instrument Avant le fonctionnement de l instrument lisez les renseignements dans cette section concernant les risques et les dangers potentiels S assurer que toute personne impliqu e avec le fonctionnement de l instrument est instruit dans les pratiques g n rales de s curit pour les laboratoires et les pratiques de s curit sp cifiques pour l instrument C Symbols and Conventions The following chart is an illustrated glossary of the electrical symbols used on th
18. to provide environmentally safe disposal If this is not available contact your local SciGene distributor or SciGene Technical Support techserv scigene com for assistance Do not treat electrical and electronic equipment as unsorted municipal waste 3 Restriction of Hazardous Substances RoHS This symbol indicates the instrument is in compliance with the European Union s RoHS 2 Directive 2011 65 EU on the restriction of the use of certain hazardous substances in electrical and electronic equipment EEE RoHS CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 6 408 733 7337 techserv scigene com 4 Electromagnetic Compatibility EMC The CytoBrite System is a Class A digital device under FCC Title 47 Part 15B and designated as Class A electrical equipment for measurement control and laboratory use EN61326 Note regarding Canadian EMC compliance Le present appareil numerique n emet pas de bruits radioelectrique depassant les limites applicables aux appareils numeriques de class A prescrites dans le reglement sur le brouillage radio
19. will appear The instrument is now ready for use D Changing Alert Sounds The CytoBrite System comes from the factory with alert sounds set to OFF Alert sounds can be turned ON to notify the user of the following events Protocol End two long beeps at protocol completion Each Select one short beep each time Select button is pressed Preheat continuously beeps upon reaching set temperature until Select button is pressed Denature one short beep indicates this step has completed To turn sounds on or off 1 From the Home screen go to Settings Sounds 2 Use the Forward button to choose an alert from the list and press Select 3 Use Forward to choose ON or OFF and press Select 4 Continue to change alert settings as in step 2 above or exit to the Home screen by pressing the Back button twice Return to Sengs Upgrading Software CytoBrite Run Create View Edit Files Settings SETTINGS Upgrade Software Insert USB press Select SETTINGS 1 Block Type 2 Calibrate 3 Upgrade SW 4 Sounds Upgrading Upgrade Complete Press any key SciGene Version x x Month Day Year CytoBrit
20. 1 37 Time 16 00 00 Incubang Only If Denaturing File100 DONE Time 16 00 00 Temp Off Preheats for 5 minutes at programmed temperature RUN Preheat Complete Load Slides Press Select RUN File100 Preheat 36 9 Temp 37 Time 00 05 00 Unit beeps to load slides File100 DONE Turn off block return to home Yes No File100 DONE Time 16 00 00 Temp 37 1 OFF HOLD No No CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 14 408 733 7337 techserv scigene com Copy Rename Delete a Protocol FILES File 37to4 Copy to USB Key CytoBrite Run Create View Edit Files Settings FILES CytoBrite Folder Select File 1 37to4 FILES CytoBrite Folder File 37to4 Copy Name Delete FILES File 37to4 Enter new name 37to65 FILES File 37to65 Finish and save Yes No Return to Home screen FILES File Location CytoBrite USB Key FILES Copy 37to4 to USB key Yes No No No FILES Delete 37to4 Yes No Copy Delete Name Yes VII MANAGING FILES A Viewing a Protocol Saved protocols can be reviewed in read only mode 1 Select View from the Home sc
21. Operation 20 to 80 RH non condensing Storage 10 to 90 RH non condensing Overvoltage category II CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 20 408 733 7337 techserv scigene com XII ORDERING INFORMATION Cat Description UoM 2019 70 1 CytoBrite Slide Oven 115V Includes ten 6 slide racks EA 2019 20 1 CytoBrite Duo Slide Incubation System 115 230V Includes two 6 slide racks EA 2019 10 0 CytoBrite Slide tray Holds 1 to 6 slides EA 2010 00 1 FISH Wash Buffer 1 0 4xSSC 0 3 IGEPAL pH 7 EA 2010 00 2 FISH Wash Buffer 2 2xSSC 0 1 IGEPAL pH 7 EA 2020 00 1 CytoBond Removable Coverslip Sealant 100 ml EA 2022 00 2 CytoZyme Stabilized Pepsin 50X Concentrate 20 ml EA 2022 00 3 CytoZyme Stabilized Pepsin 50X Concentrate 100 ml EA 2022 10 2 CytoZyme Reaction Buffer 1L EA 2022 10 3 CytoZyme Reaction Buffer 4L EA 2030 00 1 Sodium Thiocyanate Pretreatment Reagent 1L EA 2030 00 2 Sodium Thiocy
22. Use 3 B Instrument Safety 3 C Symbols and Conventions 3 D Warnings 4 E Cautions 5 F Compliance 5 II UNPACKING AND SET UP 7 A Unpacking the Instrument 7 B Parts Provided 7 C Coverslip Sealant Provided
23. anate Pretreatment Reagent 4L EA 1051 52 0 Digital thermometer Includes cable and NIST certificate EA CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 21 408 733 7337 techserv scigene com XIII DECLARATION OF CONFORMITY CytoBrite Slide Incubation System SciGene 1287 Reamwood Ave Sunnyvale CA 94089 USA Declares that the above referenced product s meets the essential requirements of the following European Union Directives by using the relevant standards shown below to indicate compliance EMC Directive 2004 108 EC EN 61326 1 2013 Electrical equipment for measurement control and laboratory use to include EN 55011 2010 Class A EN 61000 3 2 2009 EN 61000 3 3 2013 EN 61000 4 2 2009 EN 61000 4 3 2006 EN 61000 4 4 2012 EN 61000 4 5 2006 EN 61000 4 6 2014 EN 61000 4 8 2010 EN 61000 4 11 2004 LVD Directive 2006 95 EC IEC 61010 1 2013 Safety requirements for measurement control and laboratory use
24. ance at the back to attach the power cord Using only the power cord provided plug it into the back of the unit and then to a properly grounded outlet Turn on power using the ON OFF switch on the front control panel Allow 16 inches of horizontal clearance for air flow Allow 18 inches of vertical clearance to open the lid Allow 3 inches of clearance at the back for the power cord CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 8 408 733 7337 techserv scigene com III USING YOUR CYTOBRITE SYSTEM A System Components Component Function SlideSense Temperature Sensor Senses and transmits slide block temperature information to controller Temperature Controller Used to program and monitor instrument operation and report slide block temperature Slide Tray Holds slides during sample preparation and incubation Power Switch Turns on main power to unit USB Port Used for saving datalog files and installing software Hinged Lid Covers slide trays during processing Block Temperature Calibratio
25. bation temperature 3 When the preheat step is complete the display will flash Load Slides Press Select 4 Open the lid load the tray s and press Select 5 If a denaturation step is part of the protocol the unit will rapidly heat and hold at denature temperature then rapidly cool to incubate temperature warming for a preset time as programmed by the operator 6 If a denaturation step is not used the unit will simply heat to the incubate temperature where the slides will be warmed for the programmed time 7 If Off was selected as the program ending option slide block heating will turn off at the completion of the protocol If Hold was selected the slide block will be held at the programmed temperature 8 Press any key to terminate a protocol 9 Select Yes in the Turn Off Block confirmation screen to return to the Home screen Running a Protocol Return to Home screen CytoBrite Run Create View Edit Files Settings RUN Run Protocol File100 Yes No RUN Operating Mode OneTemp Protocol Yes RUN File Location CytoBrite USB RUN Select File 1 File100 2 File200 RUN File100 Mode Denature Temp 72 9 Time 00 05 00 RUN File100 Mode Incubate Temp 37
26. e Slide Incubation System USER MANUAL Version 1 0B April 2015 18 408 733 7337 techserv scigene com IX MAINTENANCE A Cleaning the Instrument Clean outer metal surfaces and the heated lid using a soft cloth and mild detergent based cleaner Avoid abrasive cleaners that can scratch surfaces Do NOT use caustic or strongly alkaline solutions e g strong soaps ammonia or bleach at a higher concentration than specified above If running radioactive or biohazardous reactions consult your institution s radiation safety officer or biosafety office for assistance Turn the power switch to the OFF position and unplug the power cord before performing any cleaning procedure Tournez le commutateur d alimentation sur la position OFF et d branchez le cordon d alimentation avant d effectuer toute op ration de nettoyage Remove liquid or condensation wipe dry while instrument is OFF and block is cool or at room temperature Enlever le liquide ou la condensation essuyer tandis que l instrument est teint et le bloc est frais ou temp rature ambia
27. e CytoBrite System Whenever such symbols appear on instruments please observe appropriate safety measures Le tableau suivant est un glossaire illustr des symboles lectriques qui sont utilis es sur le syst me Chaque fois que ces symboles apparaissent sur les instruments veuillez observer les mesures de s curit appropri es 1 Electrical Symbols This symbol indicates that this is a protected ground terminal that must be connected to earth ground before any other electrical connections are made to the instrument Ce symbole indique qu il s agit d un terminal de terrain prot g qui doit tre connect la terre avant que toutes les autres connexions lectriques sont apport es l instrument CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 4 408 733 7337 techserv scigene com CAUTION This symbol alerts you to consult this Operator s Manual for further information and to proceed with caution ATTENTION Ce symbole vous avertit consulter ce guide de l utilisateur pour plus d informations et de proc der
28. electrique edicte par le Ministere des Communications du Canada 5 FCC Warnings and Notes This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules The instrument qualifies as an exempted device under 47 CFR 15 103 c in regard to the cited FCC regulations in effect at the time of manufacture Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 7 408 733 7337 techserv scigene com II UNPACKING AND SET UP A Unpacking the Instrument The CytoBrite instrument is shipped in a double carton To unpack the instrument open the outer box take out the bag containing the manual and power cord and remove the protective cardboard corners Then open the inner box reach down the inside and carefully lift out the instrument by its base NEVER lift by the hinged lid Place the instrument on the bench and raise the lid to remove two slide trays Inspect parts for
29. ler memory or USB key and the protocol to edit 3 Select Denature Incubate or End step to edit 4 Make changes to time hours minutes seconds and or temperature 15 90 C for the selected step 5 Confirm changes then select Finish and Save or Continue Edits 6 Save edited file to memory or USB key To rename file select Save As and create new name before saving CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 12 408 733 7337 techserv scigene com V ONETEMP MODE The CytoBrite System can be operated to maintain a single block temperature from 15 to 90 C using the OneTemp mode For information on creating and saving multi temperature and multi step protocols see section V CREATING AND EDITING PROTOCOLS 1 From the Home screen choose Run OneTemp 2 Set the desired block temperature from 15 to 90 C 3 Press Select Yes to start the program Set temperatures for the block will be displayed Time will be listed as Forever as the program will continue until stop
30. n Jack Connection for digital thermometer cable to calibrate slide block temperature Power Input Module and Fuse Housing Location of main fuses and power cord connection CytoBond Removable Coverslip Sealant Seals coverslips to slides CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 9 408 733 7337 techserv scigene com B Preparing Slides Loading the Instrument Starting a Protocol Slides are prepared for molecular analysis following your standard procedures using the CytoBrite Slide Trays provided with the unit CytoBond Removable Coverslip Sealant SciGene cat 2020 00 1 is used in place of rubber cement for sealing coverslips to prevent probe evaporation without humidification 1 Place slides to be analyzed in the tray s pipet probes and place coverslips 2 Seal coverslips by dispensing a continuous bead of CytoBond Sealant around edges 3 Turn on power to the instrument select and start the desired program See section V for how to create programs 4 When the unit finishes pre heating the controller displays a
31. nte B Cleaning Air Vents Clean the air intake bottom of instrument to prevent vents from becoming clogged Remove any dust and debris airflow may be hampered effecting performance of the instrument and damage by overheating of components C Checking and Replacing Fuses There are two fuses located in a removable fuse block below the power cord receptacle on the back of the unit A blown fuse appears dark Always replace fuses with those of the same amperage and voltage as shown on the label below the fuse block Turn the power switch to the OFF position and unplug the power cord before performing any service procedure To remove fuses 1 Insert a small flat blade screwdriver into the tab recess just below the plug receptacle 2 Push down to release the fuse block 3 Slide fuse holder out from the fuse block while holding the retaining tab out of the way 4 Gently pry out the fuses Pry open tab with screwdriver Fuse holder Fuse block Push clip up to release CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 19
32. ol 10 B Editing a Protocol 11 V ONETEMP MODE 12 VI RUNNING A PROTOCOL 13 VII MANAGING FILES 14 A Viewing a Protocol 14 B Copying a Protocol 14 C Renaming a Protocol 14 D Deleting a Protocol 14 VIII SETTINGS AND PREFERENCES
33. ped by the user 4 To end the program use the Forward button to display the End Protocol screen and Select Yes The instrument will display OneTemp DONE and total elapsed time Running OneTemp Mode CytoBrite Run Create View Edit Files Settings RUN OneTemp Set Temp 73C RUN Operating Mode OneTemp Protocol RUN OneTemp Temp 73 2 73C Time 05 00 00 End OneTemp Yes No OneTemp DONE Time 00 01 01 Yes Return to Home screen No Temp 37C Time Forever Start Yes No No CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 13 408 733 7337 techserv scigene com VI RUNNING A PROTOCOL The RUN function is used to run a previously created protocol stored in the controller memory or on a USB key To operate the CytoBrite instrument at a single temperature without a timer see section V ONETEMP MODE 1 Select Run Protocol from the Home screen 2 Choose the file location CytoBrite or USB key and select the protocol The protocol will start by performing a 5 minute preheating of the block to the programmed incu
34. prompt and beeps to load slides Raise the cover place the tray s into the unit and press Select to start the program 5 The instrument then heats the slides to the programmed temperatures and times C Powering On Off To turn ON the instrument press the top of the power switch the l position To turn OFF the instrument press the bottom of the power switch the O position D Using the Controller A 4 line LED display controller is used to CREATE RUN EDIT and VIEW protocols manage FILES rename copy delete and customize SETTINGS including calibrating temperature and choosing alert sounds Navigating menus and selecting options is performed with four buttons on the display panel Home returns to the main menu screen Select chooses the option highlighted in the display Forward increments or toggles between options Back returns to a previous screen or option Details on using controller menus can be found in later sections of this manual E Using the USB Drive A USB key inserted into the USB DRIVE can be used to Capture a time and temperature log when running a protocol Allow protocols to run directly
35. reen 2 Choose the file location controller memory or USB key and protocol 3 Use Forward to advance through the protocol in the following order Denature temperature and time Incubate temperature and time Final hold temperature or OFF 4 Press the Forward or Back buttons to continue B Copying a Protocol A protocol can be copied and saved under a new name in the controller memory or USB key 1 Select Files from the Home screen 2 Choose the file location controller memory or USB key and protocol 3 Select Copy 4 Choose new file location and Select Yes C Renaming a Protocol An existing file may be renamed and saved in the controller memory or USB key 1 Select Files from the Home screen 2 Choose the file location controller memory or USB key 3 Select Name 4 Enter a new name by choosing characters space A Z 0 9 using the Forward button Sequentially select a desired character using the Select button Protocol names must be 8 characters in length including spaces 5 Select Yes to save D Deleting a Protocol 1 Select Files from the Home screen 2 Choose the file location controller memory or USB key and protocol 3 Choose Delete
36. t Create 2 Enter the name of the protocol by scrolling through the alphanumeric characters space A Z 0 9 using the Forward button Sequentially select a desired character using the Select button Names must be 8 characters in length including spaces 3 On the next screen select Yes to use a Denature step If chosen enter the Denature time hours minutes seconds and temperature 15 90 C on succeeding screens 4 On the following screens enter the slide incubation temperature and time for the probe hybridization step 5 Complete creation of the protocol by choosing how to end the protocol Selecting Off turns off the slide block Selecting Hold keeps the slides at a selected temperature until removed 6 If Hold is selected as the protocol end option enter a Hold temperature 7 Save the completed protocol to the controller memory or a USB key Make sure a USB key is inserted BEFORE selecting the USB option CytoBrite Slide Incubation System USER MANUAL Version 1 0B April 2015 11 408 733 7337 techserv scigene com C
37. ytoBrite Run Create View Edit Files Settings EDIT Select File 1 FILE100 2 FILE200 EDIT File Location CytoBrite USB Editing a Protocol EDIT File100 Denature Temp 73 Time 00 02 00 EDIT File100 Temp 73 Time 00 02 00 OK Yes No EDIT File100 Denature Yes No Set Temp and Time EDIT File100 Finish and Save Continue Edits EDIT File100 Save in CytoBrite USB EDIT File100 Save Save As EDIT File100 Save in CytoBrite Yes No Return to Home screen Save CytoBrite EDIT File100 Denature ON Incubate End EDIT File100 Incubate Temp 37 Time 14 00 00 EDIT File100 Temp 37 Time 16 00 00 OK Yes No EDIT File100 End Off Hold EDIT File100 Hold Temp 37 EDIT File100 Denature Off Yes No Denature End Incubate Hold Yes Select New Temp Finish No Yes Yes EDIT Enter Name File101 Save As O Yes Connue Edits No B Editing a Protocol The Edit function allows changes to the Denature and Incubate times and temperatures as well as protocol end options Off or Hold in an existing protocol Edited protocols may be saved under the existing name or be renamed using the Save As function to the controller memory or USB key 1 Select Edit from the Home screen 2 Choose the file location control
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