Home

UVSX Manual WD Version 2.20 21-10-07

1. Font Series Color The Style and Position tabs are most likely to be used The remaining tabs control items such as size and form of the displayed symbol and generally the applied default values will not need changing 2 9 2 3 1 Style Visible Unchecking this checkbox will remove the legend box from the chart Inverted Checking this box will cause the legend order to invert i e the last sample to run will be shown at the top of the box Checkboxes Unchecking this box will remove the checkboxes adjacent to the text in the box This is not recommended since the checkbox is very useful if a single curve should be removed from the overlay for any reason Legend Style lt is recommended to maintain this setting as Automatic per default Text style It is recommended to maintain this setting as Left Value per default Vert Spacing Controls the line spacing used within the legend box Dividing Lines Allows definition of the format of lines used between the individual legend entries within the box 2 9 3 Audit Trail The UVS software contains an integrated Audit Trail which tracks all changes made to all documents after acquisition This trail is applied to both methods and all reports and is linked to name and revision of a document E Click on the Audit Trail icon in any screen to view the audit trail window for the current document and revision A prompt will appear asking whether you wish to view the Report Trail which c
2. i i i i i i i i i i i i i i i i i i i i i i i aa 360 365 370 375 380 385 390 395 400 405 410 415 420 425 430 435 440 445 450 455 460 465 470 475 480 Chart Parameter Result Table V Instrument Control Status windows Photometer Control Cell Changer Autozero Mode 6sMr v Wavelength nm Method amp Accessory Control 580 0 set Postion 1 set Method if Acquisition status Yalues l l l Sample Wavelength nm Abs Method Holmium 360 480 0 ae SS Samples 1 11 30 03 Finished User werner Working Area Selection Buttons for selection of working window e g Method Standard Toolbar Contains buttons for starting and stopping measurements plus Excel export printing and saving data for the currently open document Graphics Toolbar Contains all functions required for controlling the appearance of the curve display these are described in more detail below Status Windows The status windows shows the current or last instrument status Method amp Accessory Control Enter the method name and the number of samples to be analysed UVS So
3. i A T Data O Data Pagel B B Customers 5 6 MasterDatal ReportTitie RepartTitie E Cust No E Shapel OUR CUSTOMERS F Company A Memo2 Z Addri A Memo3 Addr2 city State A Memo4 A Memo5 5 A Memob siCustomers Company z Customers Company j Zip Phone Customers Phone B Country FAX Customers FAX Phone a EMER gt t A Memo ReportTitlel aS B FAX E Tax Rate E Contact B Last Invoice Date 45 Memos TfrxMemoVview Properties Events h Align baNone AllowExpressioi True AllowHTMLTags _ False Auto Width _ False BrushStyle W bsSolid CharSpacing 0 Clipped True Color W cione Cursor crDefault DataField Company DataSet Customers Description DisplayFormat ExpressionDelir FlowTo Font TFont GiFrame TFrxFrame Gapx 2 Gap 1 v OLE v Fi Align Determines the alignment of the object relative to band or page Create field y Ll create caption 0s Wl ie 4 a Wet OE 8 aS a y a T Centimeters 0 40 0 30 MemoS Customers Company In the picture denoted with numbers 1 report design work space 2 menu bar 3 toolbars 4 object s toolbar 5 report pages tabs 6 Report tree window 7 Object inspector window 8 Data tree window You can drag elements to a report page from this win
4. 2 4 2 4 Calculations in Wavelength Scan The curve calculation modes are generally embedded in the method and are described above and in detail in the Calculation section UVS Software for Recalculation after for example changing a Peak Detection parameter is possible at any time by clicking on the Calculator icon followed by election of the parameter within the dialogue window Manual Version 2 20 Oct 2007 UVIKON 900 XL XS Page 61 of 202 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi U 2 4 2 5 Peak Detection Clicking on the peak detection tab will show the list of wavelength and absorbance values for the detected peaks UVS Software for Manual Version 2 20 Oct 2007 Page 62 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 4 2 6 Overlay Yat DuSoTec GmbH Dime Software Overlay is a feature to collect several charts together even the methods are not the same Tomik GmbH Clicking the overlay button a window called overlay opens Move to the overview chart press the left mouse button move to the overlay window and release the mouse button drag and drop Pressing the save button the overlay will be saved E Method Report Box g9 A BGH a w m T Name Rev Date User 67345299 _Mg_ZusatzSpektre 0 07 09 2005 kuehnn 167345299 _Mg_ZusetzSpektre 1 07 09 2005 kuehnn Photometeriiberpriifung_Aug 0 02 08 2005 kuehnn
5. 2 4 3 2 2 Common Parameters The right hand panel contains common parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change Tungsten lamp Deuterium lamp Filter change 1 5 Bandwidth Cell changer This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength When checked the tungsten lamp will be switched on and used according to the setting for the lamp change When checked the deuterium lamp will be switched on and used according to the setting for the lamp change This parameter defines the wavelength used to switch the filter Selects the spectral bandwidth of the spectrophotometer Defines the operating mode of the cell changer if fitted 2 4 3 2 3 Calculation alculation Abs T abs UVS Software for Manual Version 2 20 Oct 2007 Page 72 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DeSoTes Ombi UY The calculation selection permits definition of the mode of calculation to be used The user can select between either absorbance or transmission 2 4 3 2 4 Curve Parameters in the Time Drive Method Be Opens an edit dialogue for defining curve parameters and related formatting In Time Drive the curve parameters can be link
6. Contains buttons for navigating the current list plus buttons to allow archiving or printing of reports Contains a list of all reports available used for the given measuring mode Scrolling through the list updates automatically the corresponding fields in the results area fields Shows all curves available for the current report Displays data for the samples contained in the current report Shows details relating to status of electronic signature for a report Available Reports List Curve Display Area Comments Area Electronic Signature 23 1 6 Quick Search Function and Row Colouring In Methods and Reports a quick search function may be used to quickly find the required file Therefore click on the fixed row to enter the name of the file It will be highlighted automatically UVS Software for Manual Version 2 20 Oct 2007 UVIKON 900 XL XS Page 33 of 202 UVS SOFTWARE for UVIKON 900 XS XL N AT USER MANUAL Bednar UY PY Colibration Tawavelength BinHe n DOTAT edwi 1 O37 tied i D OH 248_calite ation _ TH cutter ation 1 01210_catbration 2 DHHG calibration a DHHS cabra d DHS cabrar 5 T gay kal are 0 re D 1 001223 best O84 223 heni Also by clicking on the small arrow in the left corner of the fixed row the rows may be coloured FP calibration Finwavelength EL 2 acpi E eoa E Report bl e e FI E vrba grodect Ee Si fy Sh Li SPE a T Tigi 0
7. L Peakdetection Peak alley w w Activity Katalase from 0 45000 Abs Decimals 1 to 040000 Abs Calculation 13 17 F T 0 05 Time interwal mir ZEC max 220 ZEC x Cancel Help UVS Software for Manual Version 2 20 Oct 2007 Page 200 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL NA nal USER MANUAL pamen The results are shown in the result tab sheet A factor per sample can be entered if the field is blanked a value of 1 will be calulated ahi Time Drive 7 dime ADS l r A z 1 1 4 7 oa S aes deel e Abs 1 Ab uml chert Table Recut Activty Parameter F wy sees Sena ERCP INES UVS Software for Manual Version 2 20 Oct 2007 Page 201 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Beire OUY The linear regression curve is shown p Time Drive V Hi Sample 1 UVS Software for Manual Version 2 20 Oct 2007 Page 202 of 202 UVIKON 900 XL XS
8. Wat Calculation Parameter i 6 xj ume Fires Line Show dese T i Shae crosapeerts M Peskdetection By activating the Calculation box a mathematical function or and a wavelength function may be implemented The Math Function may be defined with or without a reference file E g S R Subtract reference from all samples S R Add reference to all samples 1 2 Multiply all samples with 1 2 Format 1 2 or 1 2 depends on windows If individual references should be subtracted in WL Scan mode e g to subtract the reference from the same cuvette then the Calculation can be defined in the following way e Tick the Calculation Define the Math Function S R e Under No of references define the number of reference runs that should be subtracted from the current runs Example When an automatic cell changer is in use then 8 individual blank runs should be subtracted from 8 sample scans afterwards Select 8 references Measure the 8 reference runs first Add the samples into these 8 cuvettes Start again using 8 samples Then the first 8 references are being automatically subtracted from the following 8 samples UVS Software for Manual Version 2 20 Oct 2007 Page 56 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeoTes Ombi UY To use the Wavelength Function choose the number of functions and then enter the wavelength and a mathematical function to have
9. gt Post run by clicking on the Report tab in the report section and then the calculator icon in order to obtain the calculation dialogue gt Definition of the required calculations as a part of the method in which case they can be executed automatically at the end of the run Click on the calculator icon in either the Method or Report screen in order to obtain the calculation dialogue PRedit Calculation Parameter l j Ii zjojxil Curve function Smooth fv Calulaton Show dawala 1 Mone Chart Function Sheay erase C Aam O Bese fw Feskdeechon Peas Valley O Pax min Ais 1 1000 i Vaky Threshold 0 0050 i Peaky alby Achy from 300 min Factor 1 0000 he 400 mm Uras w OF X ibat 7 beo The functions available for manipulation of curve data are the following Derivatives up to 4 derivative Cross points smoothing Mathematical calculations on a complete curve Peak detection Activity VVVVV WV UVS Software for Manual Version 2 20 Oct 2007 Page 104 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS A USER MANUAL _DeSoTes Ombi UY 2 5 1 1 Derivative The software allows direct calculation of derivative orders 1 to 4 for Wavelength Scan and Time Drive data A separate optimised calculation algorithm is used for each order therefore the quality of the result will be much improved if you calculate for example a fourth derivative directly from a loaded zero order curve
10. Samples Defines the number of samples to be analysed Checkbutton Press this button to accept the number of samples the sample grid will also created Autozero Defines whether an autozero should be executed before starting a measurement Method The method box contains the name of the method and directly adjacent the actual revision number of that method The method is selected from a separate window which is opened by clicking on the button adjacent to the revision box CETER f B xj Method Revision Last Change Recharte o 30 07 2004 10 1854 Tati 0 30 01 3004 10 184 ee 0 3001204 102347 ide by usaga C alphabelicaly This window contains a list of all available methods including all revisions of the method and the date of last modification Selection is made by highlighting the required line and then clicking on OK The order of Methods may be displayed by usage or alphabetically Wavelength A certain wavelength may be chosen manually and set immediately in order to allow rapid monitoring of the unit 2 4 3 1 2 System Status Tien salu values Senple 0 wavelength nm Absorbance Readings 400 0 0 1122 Penning Di iter Tine su eee The lower right section of the display contains the actual status of the system and displays the following parameters UVS Software for Manual Version 2 20 Oct 2007 Page 70 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _D
11. XS XL N USER MANUAL DuSoTec GmbH 2 9 4 2 Signature Status Indications 2 9 4 2 1 Quick Overview There are various indications to show the status of a report and whether it has in fact been signed off and accepted All signed reports in the list are shown with a shaded background so that the user can immediately identify those reports in the list which have been signed and accepted The status of a signed report in the report list is shown automatically when the report is selected in the report list Pred wavelength ee 218 3 m 5 Acqdetion EE Method E Report Solis A BS m a gt m Mowteproiet B Mame Rev Cote User Webuee teat 03002004 admin el GS 0 0007 0 0008 LT ja Di2 0 1438 Didis o 0000 0 5835 0 5897 Ogee 00w 12355 1332 2 izi i6 Leer admin The Approval and Accepted checkboxes are only checked for a signed report The Signed Rev field always carries the revision number of the signed report if there is no entry in this field then no signed report exists for that particular named report lt is also possible to partially sign a report e g only approval and in this case only the Approval box would be checked 2 9 4 2 2 Detail Overview UVS Software for Manual Version 2 20 Oct 2007 UVIKON 900 XL XS Page 182 of 202 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Yat DuSoTec GmbH Due Software Tomik GmbH By clicking on the
12. UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL DusoTes GmbH UY l l Default Default mode The printer prints on the sheet defined in a report One preview page is printed on one sheet l gt Split big pages Split big pages This mode is useful if you need to print A3 report on A4 sheet One preview page is printed on several sheets If you choose this mode you have to choose the sheet size Print on sheet as well il l gt Join small pages d Join small pages This mode is useful if you want to print A4 report on A3 sheet Two or more preview pages are printed on one sheet If you choose this mode you have to choose the sheet size Print on sheet as well l gt i Scale Scale mode Report is printed on specified sheet All report output is scaled One preview page is printed on one sheet If you choose this mode you have to choose the sheet size Print on sheet as well After clicking on OK report printing begins If the Print to file flag is selected file name is called And report is saved to this file file with prn extension It contains a copy of information sent to printer 2 8 23 Text search in report Report Designer allows to search a set text line in a text in preview window To perform that there is button on toolbar or its hotkey analogue Ctrl F After that search dialogue appears Find Text Text to Find po Search options Search From beginning F
13. User admi v 2 Caii TP Taid L a re Set the number of samples to 4 and the Blank Samples to 1 Enter descriptions if required and insert the Blank sample in position 1 in the cell changer and the four samples in positions 2 to 5 Start the measurement by pressing the Start button The measured values will be displayed during acquisition PP Wwavelengt I progran ee L i 3 fm si 00435 oaa 01355 32 oa 05533 0 5628 me 125868 O 11358 11551 I s ars aor Foljet jado p EA l rT La a eee 1 UVS Software for Manual Version 2 20 Oct 2007 Page 101 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Yh M USER MANUAL e5 The final window will look like this Pwavelensth program eee eles E Acquistion a mis EFs a 012a 00151 01252 0S3 11355 0 0797 oma Wovelerath nm seo set ited hod Boquistion staus Values wa _ i rT canoe 700 0 0 0229 ais 74 fae Finished e Ea Note If you are not using a cell changer you will be prompted for the next sample after each sample has been measured The final appearance of the screen remains unchanged If you repeat the measurement you will always measure the selected number of samples 3 in our example until you change the Samples parameter The system will prompt if the previously run sample data has not been saved before starting a new run AN Data not saved Continue
14. the object located underneath it and linked to the Bio Length cm field was shifted down This happens because all the objects have the Shift property enabled by default ShiftMode smAlways in the object inspector Such objects shift downwards if there is a stretchable object above UVS Software for Manual Version 2 20 Oct 2007 Page 154 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL u Dussp Software Tomik GmbH them a Text object with the Stretch property enabled The height value by which the object shifts depends on how the object from above is stretched However it is unacceptable in this case since we need the object with the Length cm text to be shifted as well To perform this there is a special band type in Report Designer the Child band It is linked to and is displayed after it s parent band Add a Child band to our reports design layout and move the 2 Text objects into it umber Bio Specie ate gory Bio Category ame Bio Comm Child Childi Bio Length Link the Masterdata band to the Child band by setting its Child property in the object inspector Now each time the Masterdata band prints the Child band will be printed immediately afterward Clown Triggerish po d Snapper Red Emperor ength crm As you can see now the title is typed exactly where it
15. they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by using the button In order to do this it is first necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can then be entered 2 4 4 2 1 Acquisition parameters Delay time The instrument will wait for the selected delay time after the MEASURE key is pressed to start the actual measurement Averaging time Defines the time the instrument uses to collect data for calculation of a mean value for one reading A long averaging time is useful to reduce noise when measuring at high absorbance values Measure time Defines the actual duration of the measurement in minutes Sampling rate Defines the number of data points per minute for the measurement Autozero at start Defines whether an autozero is to be executed beforeeach sample run Auto Calculation If checked the calculation for peak detection smoothing etc starts automatically after acquisition using the defined parameters Calculation mode Calculation and curve portrayal can be in either Absorbance or Transmission units Wavelength mode Defines the amount and the wavelengths to be used during measurement 2 4 4 2 2 Common Parameters The right hand p
16. 3 1 Report Trail 179 2 9 3 2 Deleted Reports 179 2 9 4 Electronic SIQNAtUIe cccceeccceeecceeeceneeceecsceceueeseeeceucecsueesseesaueeseessueesseesaeeesseeess 179 2 9 4 1 Signing a Report 179 2 9 4 2 Signature Status Indications 182 2 9 4 2 1 Quick Overview 182 2 9 4 2 2 Detail Overview 182 2 9 4 2 3 Signature Audit Trail 183 2 9 4 3 Deleting a Signed Report 183 3 OPTIONS AND ACCESSORIES itsivcsisncvicssncisesisissctaiasacsastsncsducsannivs 184 2L AUTOMATIC CELL a eee earn ee reece eee 184 3 1 1 Installation 00 ec cece cece acess eeeeeceeeseecaeeseesaeeseeeeeseeseeesaesaeeseesaeesaeeaeeseeeaeeseeeseeeas 184 3 1 2 Cell Changer Mode only WIA ais cictiniectrccniaaiotiianioniebentetichuaioaiebeaheeteleaieehamsiides 184 3 1 2 1 6 samples 1 reference 185 3 1 2 2 6 samples 6 references 186 3 1 2 3 10 samples 2 references 187 3 1 2 4 Manual operation only UVSX 188 3 1 3 Cell Changer operation in the different system Methods cccceseeeeeeeeeeeeeeeeees 188 3 1 3 1 Auto rate assay 188 3 1 3 2 Fixed Wavelength 188 3 1 3 3 Wavelength Scan 188 3 1 3 4 Time Drive 188 3 1 3 5 Wavelength Program 188 3 2 Thermosystem TIVEIMNODOGK cccsedesasenconsicvecdnsncesennsacenedusenuecenscsecdaanseniansacesedesuserreeniayeess 190 ae a 2 9 9 a rS 190 3 2 1 1 Manual operation 190 3 3 Stirrer only LIVIA viene cncaicinvir ssn snsintaintoniordinverivanineantirdiosonetdbasmainehinvenlandiatwniarBineualoeiionmi
17. 6002 0 0 0 231 52 ay 338355 55 6047 m6 o 400 99 6162 0 30M 2004 aiiin 1104 10 Lser zlej The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list Data can be viewed in the right hand window by scrolling across the table using the mouse UVS Software for Manual Version 2 20 Oct 2007 UVIKON 900 XL XS Page 97 of 202 UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DeSoTes Ombi U 2 4 5 3 1 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The report can be printed by clicking on the Print icon on the preview page 2 4 5 3 2 Importing a report from the database aal Click on the import icon to access earlier reports in the extracted database A dialogue window for selection of the database will be opened Import database X Tear 2002 l Cancel Help 2 4 5 3 3 Filtering the report list The report list in the left window will become very long with time and it may be necessary to filter the list so that only files of interest are shown to a user using the Select Report icon d Click on the Select Report icon and a dialogue window appears which allows selection of a H view by date method
18. 900 XS XL USER MANUAL _DeoTes Ombi UY Then ask the system ask for rinsing if the rinse value gt 0 e Please position the tube with rinse fluid After confirming you can use mouse or press ENTER or BLANK for yes ESCAPE for no rinsing starts with the rinsing time After this the system ask for removing the tube for air segment if the air value gt 0 e Please remove the tube to create an air segment After confirming you can use mouse or press ENTER or BLANK for yes ESCAPE for no rinsing starts with the air time Next Sample Please insert sample no 2 UVS Software for Manual Version 2 20 Oct 2007 Page 192 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTeo GmbH U 3 6 Liquid handler The gilson liquid handler 221 221XL can be controlled by the software if the module is installed Prepare operation of liquid handler Activate in options menu the liquid handler by ticking the checkbox Select the serial port connected to the liquid handler Please check that this port is free and the baudrate of the 221 is set to 9600 Select the type of the handler and the id p Options General Audit Trail Print Controls Extras Instrument Type Serial no pilot 8 Firmware O lamp off at program end L Com Ports UK Type COM1 Con eal Handler come w 271 Thermo F C2 XL System Option Company Office Dept Logo J DelphibFrojects iw
19. AcceptSign Accept to Test Delete 08 03 2003 17 40 37 i Deleted The upper panel contains those reports contained in the list by clicking on one of the reports the detail for that highlighted report is shown in the lower panel 2 9 4 Electronic Signature The UVS software supports an electronic signature process as required under the CFR21 Part 11 Rule 2 9 4 1 Signing a Report In order to sign a report the signee must have been assigned the correct permission rights for the required action UVS Software for Manual Version 2 20 Oct 2007 Page 179 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL IUY E g In order to be able to approve a document the user must have been assigned Approver rights by the administrator in the Login screen Reports can be signed even though the signees are not actually logged into the system at the time Entry of the password is however mandatory for acceptance of any signature for Approval or Acceptance of a document F Click on the Signature icon to open the following dialogue window for signing a document WA signature Ioj x ocument MWame evisian Signed Fev Fw Test 1 4 Signature Date Full Hame Created 08 03 2003 10 07 25 Administrator f Approved n C Accepted fo FO m Login Mame Full Mame me o O Password eet Comment The process of signing is automated as far as is possible in order to sp
20. Assay 125 2 7 1 2 Create and select templates and template blocks 126 2 7 1 3 List of some predefined report templates 131 Lu Kopon dosin GARO gene eee ee een eee ees ns heels een stat Sec EEEE 132 E SN sealant tinea shen teat cain patie ativan vate eaten vases casein cate 133 2 8 1 1 Key control 133 2 8 1 2 Mouse control 133 2 8 2 VOCIIATS cirrentsairicrsacncnehinnienuhehenunehenenunnisboonehiheiidvubebonunahehelduububsnunchenesdvehuhontencheiesuehtt 134 2 8 2 1 Designer mode bar 134 2 8 2 2 Standard toolbar 134 2 8 2 3 Text toolbar 135 2 8 2 4 Frame toolbar 136 ropes CAs T aS 136 2 8 4 Designer OPTIONS ccccceccseccecceecccucceueceueceueceueeeeeceseceseceeeecueeeueeeueseusseaesnenseansnass 137 Loa PO R a EEN OE E 139 Ea FE T rE eens es nese es esis 141 i ee Fe gases A E E E E SS 143 2 8 7 1 Report objects 143 2 8 7 2 Hello World report example 144 Coo Po Ta o nreno ee ee e eee ee eee 144 269 HTML Aa0s In ihe Ted arses er enrrecsse weaning uetiointalincntneneaeiin aN 146 2 8 10 Displaying expressions with the help of the Text object ccceeccseeeseeeeeeeneeenees 147 Le TA me mn ARO OT DESIO sianar annA EA EOE n SE aidaa 148 C PE Cle N 150 EA RE G a EEE AT E AE EE EA SEA E A E E A AE ETR 151 Bd Oe Id s gt PAPRATI EEE NENTI I E FEA EAI IA AE A EE ETT TT 151 2A Bes EE E EEEE EE E P EEE E T E TEE P E E PE ETT 152 ee E A E SA 154 E EAE E eee 155 2 8 18 Headers and footers
21. E 2 4 4 1 The Acquisition Screen in Autorate Assay 2 4 4 1 1 Run parameters 2 4 4 1 2 System Status 2 4 4 2 The Method in Autorate Assay 2 4 4 2 1 Acquisition parameters 2 4 4 2 2 Common Parameters 2 4 4 2 3 Calculation 2 4 4 2 4 Wavelength 2 4 4 2 5 Curve Parameters in the Auto Rate Assay Method UVS Software for Manual Version 2 20 Oct 2007 UVIKON 900 XL XS Yat DuSoTec GmbH Dussp Software Tomik GmbH 54 59 DS 29 56 57 5 57 58 58 59 59 60 61 61 62 63 65 67 69 69 70 71 72 72 72 73 73 74 74 74 75 75 76 76 78 81 82 83 83 84 84 85 85 85 Page 4 of 202 UVS SOFTWARE for UVIKON 900 XS XL N nae USER MANUAL ULN 2 4 4 2 6 Calculation Parameters in the Auto Rate Assay Method 86 2 4 4 3 The Report Screens in Autorate Assay 87 2 4 4 3 1 The Overview Report Screen 87 2 4 4 3 2 Printing 87 2 4 4 3 3 Importing a report from the database 88 2 4 4 3 4 Filtering the report list 88 2 4 4 3 5 The Chart Report Screen 89 2 4 4 3 6 Chart Report Screen Functions 89 2 4 4 4 Calculations in Autorate Assay 90 2 4 5 Wavelength Program ccccccesccseecseeeeeeceeeceeeneeenseeeeueeeeeeseeeeeeeeeeeaeessueeseeseeesaeeeaeenes 92 2 4 5 1 The Acquisition Screen in Wavelength Program 92 2 4 5 1 1 Run parameters 93 2 4 5 1 2 System Status 94 2 4 5 2 The Method in Wavelength Program 94 2 4 5 2 1 Acquisition parameters 95 2 4 5 2 2 Common parameters 95 2
22. EE Report Bese A Hew ak we y T wrie protect T Mame Rev Date User mea 008002000 admin igsi D00 oe laim LESSE 1 Signature Approved Accepted O Signed Rey Creer yiera Chart Table ia Frished Lier ladirin i The display obtained is the Overview screen clicking on the Chart tab will display the chart as the main item which can be manipulated or edited as required The tabs for Activity and Peak Detection show a display in table form for the respective parameters The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list 2 4 4 3 2 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The report can be printed by clicking on the Print icon on the preview page UVS Software for Manual Version 2 20 Oct 2007 Page 87 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N DuSoTec GmbH USER MANUAL UY 2 4 4 3 3 Importing a report from the database al Click on the import icon to access earlier reports in the archived database A dialogue window for selection of the database will be opened import database X Tear 2002 Cancel Help 2 4 4 3 4 Filtering the r
23. Manual Version 2 20 Oct 2007 Page 90 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes GmbH UY Recalculation after for example changing a Peak Detection parameter is possible at any time by clicking on the Calculator icon followed by selection of the parameter within the dialogue window UVS Software for Manual Version 2 20 Oct 2007 Page 91 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DedoTes Gabit TL 2 4 5 Wavelength Program Wavelength Program mode permits measurements at different wavelengths and also calculations such as wavelength ratios to be made automatically for a set of samples The first samples measured can be defined as Blanks and the values of these samples will be averaged and then subtracted from each wavelength measurement of that particular sample 2 4 5 1 The Acquisition Screen in Wavelength Program Select Wavelength Program from the main menu to obtain the following display PA wavelength program i l E Acquistion EE Method EA Repet F2 Start reo on j e f Os a Ea e Inetrumend Control PRetometer Cunina VWavelerath rim oo Set stethod ee l Semele _eavetienath tren ae 350 0 0 0516 Sangles Ea METEN j T las ki This is the acquisition window from which all measurements are started In this window the following parameters must be selected before startin
24. Method the calculation of mean standard deviation RSD min max and the deviation percentage of the mean regarding sample no 1 will be shown If Concentration is activated in the Method a chart is shown with the concentration values and the regression curve 2 4 1 1 2 Instrument Control Autozero may be activated before starting a measurement 2 4 1 1 3 Run parameters The run parameters are entered in the lower left section of the screen UVS Software for Manual Version 2 20 Oct 2007 Page 42 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL Method p DuSoTec GmbH Dussp Software Tomik GmbH ie samples Samples Blank Samples Method Checkbutton Thermo System Cell Changer Note UVS Software for UVIKON 900 XL XS Hans T Blank Wale o 0000 samples Defines the number of samples to be analysed Defines the number of blank samples to be measured blank samples are always expected at the start of a run The value of the measured blank is subtracted from the actual measured value of the sample prior to calculation of concentration etc If more than one blank is defined then the system calculates the average blank value for the blanks and then subtracts this from the actual measured sample values The method box contains the name of the method and directly adjacent the actual revision number of that method The method is selected from a separate
25. Photometer berpr fung_Aug 0 02 08 2005 kuehnn PQ Test 0 12 072 PQ Test1 2 0 12 07 2 PQ Test1 3 0 12 07 2 PQ Test 4 0 12 072 PQ Testi 5 0 12 07 2005 massothk PQ Test4 0 12 07 2005 massothk Atestfall02_Projektphase 2 ol 13 07 2005 testfall02_Projeltphase2 11307 2005 massothk testfall02_Projektphase 2 0 13 07 2005 massothk testfall02_Projektphase 2 1 13 07 2005 massothk Testfall11_Projektphase 2 0 13 07 2005 massathk y massothk massothk massothk massothk 3 Remark 67345299 Mg Spektrum RIF 0 31 08 2005 kuehnn Iroa oT E seep eee betel eee pied ea See soe eser are EE E essen panto 1EM Chart Spi No xg Y Overview _ Overview Chart Table 19 07 20 UVS Software for UVIKON 900 XL XS tcusebesseckhascocdhspuncheon sth ennesksaanechkonmedsccuauhinnceskanundzonendsacanduanoodae i 1 i 1 1 1 1 1 1 1 1 i i 1 i 1 1 1 i 1 1 1 1 1 1 1 1 1 1 i 1 1 1 i 1 1 1 1 1 i 1 i 1 1 1 1 1 1 1 i 1 i 1 1 1 1 i 1 i 1 i fs 1 1 1 1 1 1 _ aie Manual Version 2 20 Oct 2007 i aa 300 30 32 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 ORR poor err BB Sample 1 BB Sample 1 dasesedsnanadecses E M Sample 1 BM Sample 1 Sample 1 D C Sampe Page 63 of 202 UVS SOFTWARE for UVIKON 900 XS XL YS
26. Software Validation Certificate 1 3 INSTALLATION and REGISTRATION The installation and registration procedures are described in a separate document For the sake of completeness the login and setup procedures are also described in this document UVS Software for Manual Version 2 20 Oct 2007 Page 9 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi TL 2 OPERATING INSTRUCTIONS 2 1 INTRODUCTION This section of the manual describes the various operating possibilities available using the UVIKON Please note that in all cases it is necessary to follow the general guidelines laid down in section 2 3 dealing with general operation Examples give a step by step introduction to all system methods and calculation modes 2 1 1 System Icons The UVS software allows direct selection of many functions via a direct click on the corresponding icon Many of these icons have the same function in different methods Saves the acquired data in the database In addition a report file will be created in the ER corresponding directory for a given method These report files can be viewed using the qrv exe viewer Print preview and printing of a report Selection mask You can select a view with date method user or name The appropriate checkbox must be checked ea Transfers data to Excel Data will be transferred directly to Excel Excel must be installed for a this function to be used Note that
27. UAL DuSoTec GmbH Dumas Software Teclewk GmbH UY F FastReport new aS Edt Report View Help Mew Mew Report E Mew Page 5 New Dialog qa 1 2 1 3 Click on File and after then on LoadInheritance to m select the list of report blocks LoadInheritance Open Strg a Save OS Lod Preview Strg P LL Page Settings new neu FixReport FU S900 SourcelexosScan fra ScanRepork fra Flv s900 Source ScanReport fra Ci Programme Uy Ss SscanReport Fra Exit Alk 3 aLe EndlessHeight False E c _EndlesstWidth irae Report Settings ElFont TFont ala general Inheritance Description Inheritance settings This report is not inherited Select the option Here you can choose your report blocks They Part change appear in the report page in small blocks E E Inherit from base report 3a Sg B Calculation Header Scan Feak Detection a F j j w j Sample Data Scan Scan Graphik Scan Raw Data If there are objects with the same name are already in the template please select Rename duplicates Inherit error Base and inherited reports have duplicate objects What should we do Delete duplicates Rename duplicates UVS Software for Manual Version 2 20 Oct 2007 Page 129 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL WS AT USER MANUAL pees m FastReport new File Edit Report Yiew Help D a MR B
28. USER MANUAL _ DeboTes Gabit U Another way to overlay is to start from the chart Press shift and left mouse button while dragging to the overlay window Now you can modify the chart and put the result into the overlay E g original curve and derivate Psor TER a BB veto Brent Bele ER A WOW a r n em H PEN DENET DE E 019 E Drag and drop F Carley d BR pr o ao a 2 qy Cuvaw Chart Tabla ee f Fimi EEA e TER 3 CACA ins EE 2 Ag YXO Ohim E jpt Ho k Seis beatin Padhas 290 0 AATAS Cer a3 Peni a E a E AAHS ENAA AA wi wW W w a A a Ey HeHh hh en Hh ne Ee Conie hrt Table lar ary UVS Software for Manual Version 2 20 Oct 2007 Page 64 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL pirate OEL fae 2 4 2 7 Joined Reports The Joined Report option offers the possibility the overlay of spectra from different runs of the same method after the reports have been saved To use the Join Report Option click on New Joined Report icon and then choose the method that you want to join your reports from Please not that only data acquired with identical method and revision of method can be overlayed and make sure that select and enter both values Click Icon to select Acquisition Method TS Acquistion a Method Report Aes 4 A m a Mew Joined Report Save Joined Report First Baseline O03 admin First Baseline 0 9
29. UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DesoTes Ombi Ly ao Japoys ay Bul sayote SI SI puy BUI xa Huo AJar Alaa Alaa SI SIU ery very long text MaA SI SIU Lae 1 Tm a wn line the shorter Let us briefly examine some other Text object properties which influence its appearance Most of these properties are available in the object inspector only BrushStyle type of object filling CharSpacing space between symbols in pixels GapX GapY text indents from object s left and top boundaries in pixels LineSpacing space between lines in pixels ParagraphGap the first paragraph line indent in pixels 2 8 9 HTML tags in the Text object Yes this object does understand some simple HTML tags Tags can be located inside the text of an object Tags are disabled by default to enable them either select the Allow HTML tags item in the object context menu or enable the AllowHTMLTags property in the object inspector Here is the list of supported tags lt b gt bold text lt i gt text in italic lt U gt underlined text lt Sub gt subscript lt Sup gt Superscript lt font color gt font color Notice that not too many tags are supported but it is rather enough for the majority of applications It is impossible to modify font size and name otherwise the text rendering unit in Report Designer would become very co
30. a blank report and place a Picture object on the report page in the object editor if it does not open automatically then doubleclick on the object Load any desired picture and click OK You can load a picture from a file or clear an existing picture Picture 160 x 160 There are some options available in the objects context menu which correspond to names of the properties in the object inspector AutoSize Stretch enabled by default Center KeepAspectRatio enabled by default If the AutoSize option is enabled the object will be resized according to the size of the picture it contains Sometimes such feature can be useful if pictures of different sizes are to be displayed This option is disabled by default for convenience The Stretch option is enabled by default This option stretches the picture inside the object Modify the object s size using the mouse and you will see that the picture size always corresponds to the object s size If this option is disabled the picture will be displayed in its original size This behaviour differs from the AutoSize option because the object dimensions are not adjusted according to the picture size which means that the object can be larger or smaller than the picture it contains The Center option allows aligning a picture inside the object UVS Software for Manual Version 2 20 Oct 2007 Page 150 of 202 UVIKON 900 XL XS UVS SOF
31. anyway Click on OK to start the next acquisition run or on Cancel to return to the original data set UVS Software for Manual Version 2 20 Oct 2007 Page 102 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _ DeboTes Gabit 1 Printing Click on the Print icon to obtain a preview of the printed report which will always contain all measurements If the result is acceptable the report can be printed by clicking on the Print icon on the preview page Saving Click on the Save icon and the system will prompt for a file name SAVE ACGUISHIOR 7 Name Rev iD ate User Project ID A Ammoniumreihe 11 10 07 0 11 10 2007 11 28 02 LJ_Test_Fix_1 4 29 08 2007 07 59 04 admin li Probe Chrom Vl Blindwert als Referenz 0 11 10 2007 14 32 01 LJ Probe Chrom VI sequentiell 0 11 10 2007 14 27 23 LJ Test_RC1_1 0 18 09 2007 16 25 33 LJ Projekt Test_RC1_1 1 19 10 2007 14 21 57 LJ Projekt 4 Project Jf OK Cancel Enter the file name and click on OK UVS Software for Manual Version 2 20 Oct 2007 Page 103 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DeSoTes Ombi U 2 5 CALCULATIONS The UVS software contains various calculation possibilities depending on the selected measuring mode These calculations are accessible using the Calculator icon 2 5 1 Curve Functions Calculations based on charts can be executed in one of two ways
32. before starting a baseline but can only be edited in the method editor itself thus permitting tracking of baseline conditions during normal operation Click on OK in order to start the baseline measurement the unit will position itself at the correct starting wavelength and then automatically start the run Bazeline run Please wait Lambda 305 5 nm to 300 nm UVS Software for Manual Version 2 20 Oct 2007 Page 27 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N nae USER MANUAL U Duss Software Tay Tomik Geb UY 2 2 6 Power on Switch on the power supply to the instrument PC and monitor The Uvikon has an indicator showing that the power is on From the Windows Desktop click on the shortcut to display the start up screen and login dialogue 2 2 7 Self test and system software initialisation The self test takes about 2 minutes max and performs the following checks Program memory Data memory Tungsten halogen lamp on Deuterium lamp on Data transfer Wavelength drive VVVVV V During self test you will see the main software window on the screen with Initializing instrument displayed on the bottom status bar Exit Register Help Tiga Fixed Wavelength fil Wavelength Program Wavelength Scan 2 Time Drive A Auto Rate Assay Calibration gt Baseline Run my Option 4E Backup h Login 12 a Initializing instrument After completion of the initialisation this message wil
33. curve with respect to the last value For the point n on the curve at a time t and an absorbance value of Abs the activity is calculated as Activity Abs ADS n 1 tn tin 1 2 5 3 Concentration Factor This is the most simple calculation method for concentration and is used in Fixed Wavelength The concentration factor must be determined in advance if this calculation is to be used The system uses this factor and performs the following calculation Concentration Measured Absorbance value x Factor The factor is entered as a parameter in the Acquisition screen Click on the parameter tab to enter the screen and then enter the required value for the factor In this case no calibration file is required and the factor used is recorded in the report 2 5 4 Wavelength Program functions The Wavelength Program calculation functions apply only to this mode of operation and are described in the Wavelength Program section of this document One more parameter appears in the calculation column 2 5 5 Linear Calibration The Calibration mode is used to create standard calibration files for the Fixed Wavelength mode The Calibration uses a standard Fixed Wavelength method which must be created before running Calibration mode Method creation is possible in both the Fixed Wavelength and Calibration method screens A method can be used to create as many calibration files as needed the method and revision used is always linked to t
34. ee eae e Acesetion E Method E Report Sols J WC rmn a H FF wrie protect il F Merrie Rev Date User 0123456764 Remark Signature Approved 7 Accaplad Signed Fey Creer yka Chart Table iss Frished Lise admin The display obtained is the Overview screen clicking on the Chart tab will display the chart as the main item and this can be manipulated or edited as required The tabs for Activity and Peak Detection show a display in table form for the respective parameters The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list 2 4 3 3 2 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The report can be printed by clicking on the Print icon on the preview page UVS Software for Manual Version 2 20 Oct 2007 Page 74 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DeoTes Ombi UY 2 4 3 3 3 Importing a report from the database Click on the import icon to access earlier reports in the archived database A dialogue window for selection of the database will be opened Import database X Tear 2002 Cancel Help 2 4 3 3 4 Filtering the report list The report li
35. form of charts as shown on the monitor RichText object displays a formatted text in Rich Text Format RTF CheckBox object displays a checkbox with either a check or a cross String Grid object displays data in the form of grid OLE object is able to display any object using OLE technology The basic objects you most likely need to work with are the Band and Text objects You will learn about their capabilities in detail further in this chapter UVS Software for Manual Version 2 20 Oct 2007 Page 143 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _ DesoTes Gabit U 2 8 7 2 Hello World report example The report will contain one inscription only Hello World Open the report designer After that click the Text button in the Objects designer panel Move the mouse cursor to the desired place on the page and click again The object has been inserted The text editor window will be displayed right away if it does not appear this can be set in the designer settings then doubleclick the object Enter the Hello World text and then click the OK button Memo E mfx Text Format Highlight ool L F r Y Hella orld The report is created To preview it either select the File Preview menu item or click the corresponding button in the toolbar The preview window containing a report page with the Hel
36. from the Report Options menu A dialogue has three pages In the first page you can see the general settings of the report Report Settings General Inheritance Description Printer settings Defautt a Microsoft Office Document Image Writer on Microsoft Document Imagir SS Canon iP4200 on USBO01 Copies t Collate copies General Double pass Password fs Print iF empty Cancel You can tie a report to one of the printers installed in the system This means that report printing will be performed by the selected printer by default This might be useful in case when there are several different printers in the system e g text documents can be tied with monochrome printer while documents with graphics to the color one There is the Default printer item in the list of printers UVS Software for Manual Version 2 20 Oct 2007 Page 139 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS Aq USER MANUAL _DesoTes Gabit UY When this item is selected the report will not be tied with any printer and therefore printing will be performed by a printer which is set as the default one You can also set number of report copies to be printed and specify whether it is necessary to perform collation The values which a user sets in this dialogue would be displayed in the Print window If the Double pass flag is selected report s formation will be performed in two steps During
37. into the text in place of the expression The Text object can contain any number of expressions together with a usual text Both single variables and expressions can be enclosed in brackets for example 1 2 3 4 Any constants variables functions and DB fields can be used in expressions We will learn more about these features later in the chapter Report Designer automatically recognizes expressions enclosed in square brackets in the text But what should be done if our object contains square brackets and we do not want them to be considered as expressions For example if we need to display such text as following alt 10 Report Designer considers 1 as an expression and displays the following al 10 that is not what we want of course One of the ways to avoid such a situation is to disable the expression Just disable the AllowExpressions property Allow Expressions in the context menu therefore all the expressions in the text will be ignored In our example Report Designer would then display exactly what we need a 1 10 Sometimes text is required to contain both an expression and a text in square brackets for example a 1 myVar UVS Software for Manual Version 2 20 Oct 2007 Page 147 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi UY Disabling of an expression allows us to display square brackets in the required place but it also disab
38. is rather large Furthermore if a large band does not find room on a page it is transferred to the next one and much unused space remains at the bottom of the page This is shown at the following illustration To limit paper wastage let us use a Report Designer feature which paragraphs the band s contents All we need do is to enable the AllowSplit option of the First level data band You see that there is less white space at the bottom of report pages UVS Software for Manual Version 2 20 Oct 2007 Page 152 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes GmbH U How does band splitting work There are some objects in Report Designer which support this feature They are the Text Line and RichEdit objects They can be split while other objects cannot When Report Designer comes across the need to split a band it performs it in the following way displays the non splittable objects which find room on white space partially displays splittable objects text objects are displayed in a way that all lines find room in the object forms a new page and continues object displaying if a non splittable object does not find room on whitespace it is transferred to the next page at the same time all the objects located underneath are shifted according to need the process continues until all the band objects are fully displayed The splitting algorithm
39. is supposed to be In order to avoid child band s transferring to the next page which basically means it will be widowed from the parent band enable the Keepchild property for the parent band KeepChild in the object inspector 2 8 17 Shifting objects You have already seen how the Shift property works Let us look at the next mode of shifting Shift on overlapping In the object inspector the ShiftWMode smWhenOverlapped property value corresponds to this mode Thus object shifting will be performed in case when the object from above overlaps the given object during stretching Three cases are shown in the illustration below As you see the bottom object with the enabled Shift when overlapped option shifts only in the latter case i e when there is much text in the top object and it overlaps the bottom one UVS Software for Manual Version 2 20 Oct 2007 Page 155 of 202 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL DuSoTec GmbH Dussp Software Tomik GmbH I o If the Shift option is enabled the bottom object will be shifted anyway i l m in amp O RI This allows one to create rather complicated report output by using the logic of the object s design properties especially if an object overlaps several other objects at the same time Thus in the following example both of the upper objects contain stretchable texts and the bottom one has the enabled shifting when
40. object works Alignment buttons are located in the Text toolbar and allows one to set horizontal or vertical text alignment Pay attention to the Block Align button this button let s you align the paragraph on both object edges To do this the WordWrap property option must be enabled This is avery very very long This is avery very very lang This is avery very very long text line text line text line And this is another line the And this is another line the And this is another line the shorter one shorter one shorter ane This is a very very very long text line U And this is another line the This is avery very very long shorter one text line And this is another line the shorter one This is avery very very long text line And this is another line the shorter one All the text in the memo can be rotated at any angle within the limit of 0 360 degrees The button in the Text toolbar allows you to quickly rotate the text at 45 90 180 and 270 degrees If you wish to rotate the text at any other value use the object inspector The Rotation property sets the required angle When rotating a text setting values other than 90 180 270 the text can exceed bounds of the object as in our case see the picture below Let us increase object height a little so that the text would fit the object UVS Software for Manual Version 2 20 Oct 2007 Page 145 of 202 UVIKON 900 XL XS
41. offers several possibilities for printing and formatting the various reports and data available from the system Individual print outs are possible for method parameters individual results e g peak detection complete reports and curve data Each window contains its own Printer icon which gives a preview of the print out Within the preview page the user also has the possibility to save reports under user defined names and these reports can be viewed using the viewer of the UVS software Click on the Print icon in the report screens and you will be prompted for selection of the report type required pe Acquisition E Method E Report ri is ES A Small Report Extended Report REE Report Method zi pee eu Peak Detection Single Report 3 13 10 2007 Table 1 02 09 2007 sca 2 02 09 2007 acaz 0 26 08 2007 aca noan NA Pon The window appears it is printing dialogue UVS Software for Manual Version 2 20 Oct 2007 Page 118 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS aT USER MANUAL _ DesoTes Omit UY Printer Mame Sy Microsoft Office Document Image Writer ka Properties where Print to File Pages Copies all Number of copies O Current page Enter page numbers and or page ranges separated by commas For example 1 3 5 12 Other Print mode a gt Default Order Direct 1 9 al Duplex Default ka Print on sheet Default Let s look at options available in this dialogue
42. permits a backup or archive of either all data or an extract from the original database to a database sorted by year is possible The backup can be made to 2 independent servers via network if the appropriate directory locations have been entered in the options section ain MB eee ast Backup from famin date f 7 03 2003 16 45 16 BackUp Archive trom database Year 2003 al Archive ackup following tiles Beak k Backup Clicking on the Backup button will execute a backup of the complete database to the directory locations defined in the Options menu The right hand panel will display a listing of all files which will be backed up during the process A confirmation dialogue is shown after successful execution of the backup Use x Backup sucessful Compression rate 95 4 The backup simply saves a copy of the working database files to the defined locations from which they can also be restored at any time as described below 2 2 3 2 Restoring Backup Data Restoring of previously backed up data is possible using the restore UVS application located in the UVS directory In order to restore data you must close the normal UVS software before starting the restore process a prompt will remind you of this before continuing UVS Software for Manual Version 2 20 Oct 2007 Page 19 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL NS OT USER MANUAL Trivia Start the restore UVS program a prompt
43. pitch Locate in grid Modifies sizes location of the selected objects so that crosspoints they would be located at grid crosspoints Zoom Sets the zoom 2 8 2 3 Text toolbar con name Desno O OO No style Style Allows to select a style To define the style list call the Report Styles menu item Font Allows to select font name from the drop down list Stores last five fonts previously used Font size Allows to select font size from the drop down list Size can also be entered manually Enables disables font bolding Enables disables italics u Underline Enables disables font underlining Selects font color from the drop down list B i A UVS Software for Manual Version 2 20 Oct 2007 Page 135 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi UY selected Text object S tettatonment_ Enablestetettaignment Centratonment Enabes tert center aigon Aetatonment Erabies toargntaigmen usiyoywon Enables toasting by with m Topatonment Enablestettopatnment m Heiehtatonment Enabiestextheehtalonment m Bonom aigron Enables tert botom aignment Selects line color from the drop down list Line style Selects line style from the drop down list Line width Selects line width from the drop down list 2 8 3 Align toolbar S TF a l op oe 4 teon Description UVS Software for Manual Version 2 20 Oct 2007 Page 136 of
44. possible to set the following parameters Min Abs The Min Abs parameter defines the absolute minimum value for a peak or valley to be detected any peak or value with an absorbance value lower than the defined value will be ignored Threshold The threshold is the difference in height on the Y axis between two peaks or valleys If the difference between valley and peak is lower than the set threshold then the peaks valleys will not be detected UVS Software for Manual Version 2 20 Oct 2007 Page 106 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL NA A USER MANUAL a UY Peak Valley The selection defines whether the calculation is to be made for peaks only valleys only or both peaks and valleys If peak detection has been selected the results can be seen both in the peak detection table and directly on the curve itself 2 5 2 Activity This calculation mode can be used in the system methods Time Drive and Auto Rate Assay An activity of the second reaction can be used exclusively in the system method Auto Rate Assay It is used to calculate the activity of reactions Calculation of the activity is made using a linear regression model The calculation can be embedded in the method such that it is performed automatically at run end or it can be executed manually after acquisition Selection of the parameters for the Activity calculation is made as above by clicking on the Calculator icon in either the Method
45. smoothed points are computed by replacing each data point with the value of its fitted polynomial For the smoothing functions Average and Savitzky Golay three smoothing degrees can be selected Low Medium High 2 5 1 4 Calculation The calculation function allows definition of mathematical functions which can be used to modify the curve data S is used to denote the sample itself and calculations are defined simply using S and the appropriate factor F and signs Addition Enter F S Subtraction Enter S F Multiplication Enter F S Division Enter S F For example it is also possible to subtract an offset from each point in the curve and then double the signal level and this would be programmed as follows Click on the Calculation checkbox and then enter the required formula in this example the formula would simply be 2 S 0 2 Caution Please note that spaces are not permitted in the formula 2512 Peak Detection This calculation mode can be used with the system methods Wavelength Scan Time Drive and Auto Rate Assay Peak detection can be linked to a method and carried out during or after measurement To activate peak detection select Peak detection in the calculation parameter editor window A peak is detected when the slope of the curve changes from positive to negative a valley is detected when the slope changes from negative to positive After selecting Peak detection it is
46. the first pass a report is formed and is divided into pages but the result is not saved anywhere In the second pass a standard report formation with saving a result in the stream is performed Why two passes are necessary Most often this option is used in cases when in a report there is a need for the total number of pages in it i e the information of the Page 1 of 15 type The total number of pages is calculated during the first pass and is available via the TOTALPAGES system variable The most frequent mistake is an attempt to use this variable in a single pass report in this case it returns 0 Another use is to perform any calculations in the first pass and display the results in the second pass For example when it is required to display a sum in the group header which usually is calculated and displayed in the group footer Calculations of this type are accomplished by writing report script code in the OnBeforePrint event of an object The Print if empty flag allows construction of a report which contains no data lines If this option is disabled blank reports would not be constructed The Password field enables setting of a password which must be entered before opening a report On the second page you can setup the report inheritance options Report Settings General Inheritance Description Inheritance settings This report is not inherited Select the option Don t change I
47. the required line and then clicking on OK The order of Methods may be displayed by usage or alphabetically Wavelength A certain wavelength may be chosen manually and set immediately in order to allow rapid monitoring of the unit UVS Software for Manual Version 2 20 Oct 2007 Page 82 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL sellar UY 2 4 4 1 2 System Status ot j pear ar a aaa co i rears i rT P 400 0 0 10 1122 22 The lower right section of the display contains the actual status of the system and displays the following parameters Current sample being measured No of Readings Remaining Time also as progress bar Actual wavelength Actual absorbance value Temperature where applicable VVVVV WV 2 4 4 2 The Method in Autorate Assay Click on the Method button in the upper left corner to open the Method window M auto Rate Assay A 031217_autorateassay1 031217_autorateassay1 E 031217_autorateassay1 031219_Htest_ara1 The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not UVS Software for Manual Version 2 20 Oct 2007 Page 83 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS A USER MANUAL Bereli UVY Caution If measurements have been used the method will be locked and can no longer be changed However
48. the revision index will be incremented Method revision If this is checked a method cannot be changed All modifications are saved in a copy of the original method and the revision index will be incremented Normal Trail A trail is saved with the information user time and what has changed Extended Trail This is the normal trail with the additional information of old value and new value UVS Software for Manual Version 2 20 Oct 2007 Page 15 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS qT USER MANUAL _ DesoTes Omit UY 2 2 2 3 Print Control options This window is accessed by clicking on the Controls tab and allows control of chart print format chart colour decimal points for diplayed absorbance values and audit trail General Audit Trail Print Control Extras Projects Print Export from preview Mo Preview HTML Show print dialog RTF Excel Chart Color AML Excel CSW g H Siope Tex Decimals Decimals for absorbance Print Chart as Bitmap Normally the chart will be printed with vector graphic but if your printer driver don t print the graphic try this option No Preview If you check this option the printout will be sent direct to the printer otherwise the data is displayed with the preview on the screen Chart colour Colour table for curve colours in the order in which the curves are acquired The colours may be selected individually Decimals for absorbance Displayed and rou
49. the right mouse button to show the selection possibilities Choose Select Template Sefect emnpfaie Scan _Calculation_Graphics IF Scan _PeakDetecton_ Calculation _ Graphics Scan_PeakD etection_Graphics Scan _AawData kK Scan_SmallReport sign UVS Software for Manual Version 2 20 Oct 2007 Page 123 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL DuSoTec GmbH wre Tonik GmbH UY Confirm wtih OK Button Now you can see the templates in one print report Confirm all your actions with the green post button on the top F Edit Template O Fix Wavelength Wavelength Program Write protect DK Template groups Templates confirmation Auto print Template 1 Template 2 Template 3 a acquisition Scan_PeakDetectio ScanMethod sign Scan_PeakDetectio ScanMethod Scan_PeakDetectio Single Scan_PeakC ScanMethod Scan_RawData A Scan a we Acquistiog E Method E Report reg HA A ______ All the template cells are listed in the approbiated application Rev Date you can add as many as you want to 18 To select one please go to the application and click on the Small Peport Exterided Report Report Method Peak Dakeckion report button and then on the print symbol Single Report 3 1310 2007 Table 1 02 09 2007 BES 2 02 09 2007 sca 0 28 08 2007 L_a nan na onn UVS Software for Manual Version 2 20 Oct 2007 Page 124 of 2
50. user or name Select Acquisitions q x V Range from 13032000 gt to j13032000 Iv Method 031218 calibration Method Revision Name PO User The appropriate checkbox must be checked and where applicable either a date or name selected Clicking on OK will cause only those reports which match with selected criteria shown in the list of available reports UVS Software for Manual Version 2 20 Oct 2007 Page 98 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes GmbH UY 2 4 5 3 4 Audit Trail in Wavelength Program Report ae Click on the Audit Trail icon to view the audit trail window for a report 2 4 5 4 Calculations in Wavelength Program Wavelength Program contains alongside the basic wavelength calculations described above two additional calculation methods for the final result determination gt Blank gt Factor In Wavelength Program the Blank and Factor calculations can be executed automatically without the need to create a calibration file Blank The measured values for sample blank at each wavelength is subtracted from the measured value of each measured sample at the same wavelength Factor The actual value obtained from the measurement is always multiplied by the factor parameter This is accessible by clicking on the Parameter tab in the acquisition screen The actual calculation is based on the following formula at any given wavelength RW F V
51. values The button is not active if the margins are already set to their default values View Margins This checkbox allows the margin display in the preview to be switched on or off as required Detail Moving the scroller between normal and more increases the degree of detail shown in the curve display print out For example for greater detail on the axis display move the cursor towards more until the required detail is displayed Proportional This returns the print out to its original default settings without affecting the detail selected in the detail scroll bar 2 9 2 Editing and Formatting of Charts The software uses a built in editor which offers comprehensive formatting capabilities for both 2D and 3D reports Access to this editor is obtained by clicking on the Edit icon This icon is available in all screens including the Acquisition screen Use of the Edit facility when programming a method allows immediate control of the appearance of the result without the need for time consuming manual manipulations A Help function is provided within the Editor and therefore this document will only describe the more common needs with respect to formatting of charts the user is referred to the Help function for additional information 29 2 1 Changing the scaling Axis The axis scaling is the first available tab in the Edit dialogue box when opened Separate parameters can be defined for each axis and normally users would only be concerne
52. will appear to check that the main program has been closed C Have you closed the UYS program 7 If not please close it before continuing with Restore Close if necessary and click on OK to obtain the restore dialogue Restore database UYS Ioj x VTA Database path C Program Files UVSe database Files restore Restore Specify the location from which you want to restore the data and click on the Restore button You will be prompted to enter your name Please enter your name This name is required for record purposes and does not correspond with the user names installed for the main UVS software Enter a name and click on OK The restore process wil be confirmed x Restore successfully Finished 33 files of 33 restored UVS Software for Manual Version 2 20 Oct 2007 Page 20 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL DuSoTes Ombi U 2 2 3 0 Archive The archive is based on data obtained within any given year e g all data collected during 2003 The data is then archived in the database and the original data removed locally Caution lIt is possible to restore Archive data for viewing and modification during a working session However the restored data is automatically removed locally whenever Exit is executed Modifications made during a session are automatically added to the archive data when exiting The archive is not intended for storage of d
53. will become clearer if to look at the illustration Before split After split n if lt should be noted that the splitting algorithm is not perfect and quality of the output report may not be as expected You should use this option very carefully in cases when objects on the split band are grouped in a complicated way and or their font sizes differ Here is the example of what could be received UVS Software for Manual Version 2 20 Oct 2007 Page 153 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _ DeSoTes Gabit U in E o bo 2 a 4 5 1 2 8 16 Child bands Let us examine the case when one of the lines in a report of label tyoe may have a variable size To simulate the situation using our example let us reduce the Bio Common Name object width to 2 5 cm and enable the Stretch option for it Let us also enable stretching in the First level data band Enable all the frame lines in all objects so that the principle of the stretching function will become clear This will output a report with the following appearance umber to oo FI m ategory nggertish O Lt a Jij 4 low ength cm Moge 030 apper ed Emperor gali wo i ateqory 0 TS poz D z D o o T ength cm You see that in the first case the first object contains longer text and this is why it was stretched into two lines Thus
54. 00 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL proT OET 1 2 8 19 Report viewing printing and export The built report can be displayed printed or exported into one of the supported formats Everything can be performed in preview window p Preview d EHAA gR z Qo ABUR NM A rolt TINE Foul Boa H PFRIIIATTr aT re rr Page 1 of 2 On the picture with figures the following is displayed 1 ready report pages 2 toolbar 3 status line The following buttons are on the toolbar d g HGA AAS j Qopa Uy nu al Po Name Description Name Description Print report Prints report Hotkey analogue Ctrl P Open repot Opens file with ready report fp3 UVS Software for Manual Version 2 20 Oct 2007 Page 158 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi UY Report export Exports report to one of the supported formats Export to PDF Exports report to Adobe Acrobat file pdf This button is displayed if corresponding export filter is installed Send via e mail Exports report to one of the supported formats and sends it via e mail as enclosure This button is displayed if corresponding export filter is installed Text search in report Hotkey analogue Ctrl F DI Zooms in the preview conditions perform double click on report a Page number Transfer to report page with pointed number Enter number and click E
55. 02 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL Kenedi 1 YV 2 7 1 1 1 How to print single curves in the applications Scan Timedrive Auto Rate Assay F Edit Template E Template groups Templates O Fix Wavelength Wavelength Program _ Write protect ja Scan Name Method Template O Timedive P DE Autorate Assay Calibration Overlay O Trail General Report Templates Auto print Template 1 Template 2 Template 3 after acquisition A Small Report Scan inl i Select Template Extended Report Check Uncheck as single Report Method Scan_PeakDetectio ScanMethod Peak Detection Scan_PeakDetectio Single Report Single Scan_PeakC ScanMethod Table Scan_RawData Define the apperance of 2 curves in the print report in one diagram or only a single curve in each diagram To have only one curve in your diagramm click with the right mouse tab on your template and select Check Uncheck as single Now you have a new diagram for each curve in your print report see on the bottom Single curves are only possible in the following applications Scan Auto Rate Assay and Timedrive UVS Software for Manual Version 2 20 Oct 2007 Page 125 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL TURLI Duma Software Teclewk GmbH IU 2 1 1 2 Create and select templ
56. 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeoTes Ombi UY B _ Center across uustity vertically C hwe Ca owe SSS 2 8 4 Designer options Set the designer options via the View Options menu command UVS Software for Manual Version 2 20 Oct 2007 Page 137 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTes Ombtt UY Designer Options rid Type Size Centimeters cm Show grid Inches 0 1 in Align bo Grid Pixels pt Dialog Form pt Fonts Memo editor SIZE 10 yt Use object s Font settings Colors Workspace J toolwindows J LCD grid color Other Show editor after insert Show band captions Show drop down Fields list Free bands placement Gap between bands Restore defaults Here you can set the desired units centimeters inches pixels and specify grid step for each unit Tip You can also switch units in the designer by double clicking on the left part of the status line where the current units are displayed You can also specify whether grid should be displayed and align to grid This can be done by buttons in the Standard toolbar of the designer as well You can set a font for the code editor window and for the Text object editor If the Use object s font settings option is enabled the font in the text editor window would correspond with the font of the object
57. 21 cr ion IAHE m aiki DH caib k Wir chri Ha kal sere i GAS rairai ene D9223 best 2 3 2 Starting a measurement H Start m Stop x p eg The Start button on the main menu bar is used in all methods to start measurement of one to ten samples All parameters defined in the selected method are then used for a measurement Measurement can be stopped at any time by pressing the Stop button on the main menu bar All data measured up to that point is valid and can be stored and used for further processing 2 3 3 Recalculation UVS Software for Manual Version 2 20 Oct 2007 Page 34 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL _DesoTeo GmbH d LY bd The calculations are made automatically as part of the acquisition process in the UVS software In cases where parameters are changed a recalculation can be forced as described below Clicking on the Calculator icon on the tool bar is used throughout the software package to initiate a re calculation of one to ten samples Only the parameters in the calculate column and the display column of the acquisition parameter set are used for calculation 2 3 4 Saving data H Start otop a B Clicking on the Save button on the toolbar allows the currently displayed data to be saved The following dialogue box appears SAVE ACGUISHOR Name Rev Date Project A Ammoniumreihe 11 10 07 11 10 2007 11 28 02 LJ_Test_
58. 4 5 2 3 Wavelength Program calculations 96 2 4 5 2 4 Calculation 97 2 4 5 2 5 Audit Trail in Wavelength Program Method 97 2 4 5 3 The Report Screen in Wavelength Program 97 2 4 5 3 1 Printing 98 2 4 5 3 2 Importing a report from the database 98 2 4 5 3 3 Filtering the report list 98 2 4 5 3 4 Audit Trail in Wavelength Program Report g9 2 4 5 4 Calculations in Wavelength Program 99 2 4 5 5 Wavelength Program Measurement Example 100 ks LULAT aisi EAE 104 Lai LUNE FUNTON ern eens 104 2 5 1 1 Derivative 105 2 5 1 2 Show cross points 105 2 5 1 3 Smoothing 105 2 5 1 4 Calculation 106 2 5 1 5 Peak Detection 106 e I A EE EE renee ene rerrTs meter etree este eT ee 107 2 5 2 1 Activity Results 111 Coa Cle 111 Gs Sa FATO aE 111 2 5 4 Wavelength Program functions o nisvcsvintserasscnsiceamendspansacninpeanciaiaancnapeanaianenniagenaienes 111 Ea G ge Wer T EE EE Pnerienn ener enn perenne terry re 111 2 5 5 1 Creating a Calibration File 111 2 5 5 2 Using a Calibration Curve 115 25w Cuadra Bs 1 61 eo 116 serei EEEE a 117 e PE E EEEE 118 e P EEA EEEE E EEEE A EEEE EEE EEA EEEE 118 ar REPORT AN GER oriire E a EEEE EEE REEE 121 UVS Softwarefor gt Manual Version 2 20 Oct 20077 Page 5 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL TUS Ce T T EEEE E EAEE TEE EER ETENEE EA EEEE 122 2 7 1 1 1 How to print single curves in the applications Scan Timedrive Auto Rate
59. 4 Lal ee Nestle Folie o 209 201 ET Nestle Folie 0 20 9 20 _ Nestle Folie 1 23 08 2007 LJ Projekt O Fix Wavelength Nestle Folie 0 20 09 2007 LJ Projekt Polystyro F o 14 092007 admin O Wavelength Program C write protect Polystyrol F 1 18 09 4007 admin Scan T Name Overview Chart Peak Detection Resutt Table O Timedrive Lg Special 11 13 06 User admin O Autorate Assay H Standard Mot for resale 1 1 1 r 1 1 1 1 r 1 1 ee ee pri 1 1 1 r 1 1 1 1 r 1 1 ee 1 1 1 r 1 1 1 1 r be L Aene llel 1 D 1 r 1 1 1 1 r ane ee EE Sr E E EE PR 1 1 I 1 1 1 1 1 1 ee tee Nee eS Se ee Ss Oe SI 1 1 1 1 1 1 1 Page 1 of 1 UVS Software for UVIKON 900 XL XS Calibration O Overlay Template groyps Templates lt gt gt a e 3 Method Template Report Templates Manual Version 2 20 Oct 2007 Sal report amp Auto print Template 1 after N acquisition N Scan_SmallReport The layout of the print report is defined in the templates Page 121 of 202 UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL Bednar 1U S4 Show only active methods REVISION USED Method not active Oo 0 Lamp change 338 nm Parameter Tungsten Lamp M Lower i X nm v Aavan 358 Deuterium Lamp Upper 480 ee Filter change1 280 nm Wavelength Fiter change 2 3
60. 6i 1 OF 0S2003 admin r2 0 2611 2003 admin 52 0 2611 2003 admin O 2641 2003 admin 3 OS 05 2005 admin Select Acquisitions L Range from 13 09 2000 a to 13 09 2000 A Method Method Revision Name User Select dataset from the Overview List and then add the file by using the right mouse button The selection of datasets can be monitored in the card Joined Report during the process After the completed selection of your datasets save the created Joined Report File The overlay is saved as a normal report so that all mathematical and graphical functions are available UVS Software for Manual Version 2 20 Oct 2007 Page 65 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL test magnum overlay 0 08 02 2004 E test magnum overlay 2 0 08 02 2004 test magnum1 0 08 02 2004 test magnum2 0 08 02 2004 Add data with right mouse click DuSoTec GmbH TUY BB Sample 1 Sample 1 Sample 2 Sample 5 Sample 6 Sample 7 Sample 1 imple 2 Sa Sa IPle 5 Sample 6 N Yes Yes Yes Yes No Double click to unselect UVS Software for Manual Version 2 20 Oct 2007 UVIKON 900 XL XS Page 66 of 202 UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL Dado Tes Guba TL 2 4 2 8 Wavelength Scan Measurement Example Scanning measurement of one blank and five samples
61. 70 nm Scaninterval 0 5 v nm Fiter change 3 480 nm l f Fiter change4 570 nm Scan speed 100 v nmin Filter change5 710 nm Autozero at stat 60 480 langsam 2 z mium 360 480 lana gt Sides daton Response Time 0 1 s v aoe ee i misson i Caleulation celchanger 10s E Holmium 365 schnell Ref Suk u Abs T Abs 4 _ Holmium 36 chnell Ref Suk 11 Liquidhandler _ Holmium fu 0 ay 6 JP sk 279 Holmiur als i iy ea Ableitung x Polystyrol Folie tr b 0 Print Template IPaTestia 0 7 pecs E Pre define the print report types in POTe tib u Special 4 lt PQTestd Standard be the methods Created from admin Date 18 04 2006 14 39 34 Last modification p from admin Date 18 04 2006 14 42 34 admin User 11 11 45 2 7 1 Templates F Edit Template Ia Fix Wavelength Wavelength Program Write protect x a Scan Name Method Template O Tinedive Autorate Assay i Calibration Overlay O Trail General Template groups Templates L Application Database view select Report Templates 4 Auto print Template 1 Template 2 Template 3 after acquisition Small Report Scan_PeakDetectio Extended Report Scan_PeakDetectio ScanMethod sign Report Method Scan_PeakDetectio ScanMethod Peak Det
62. Acquisition parameters 2 4 2 2 2 _ Common Parameters 2 4 2 2 3 Calculation 2 4 2 2 4 Curve Parameters in the Wavelength Scan Method 2 4 2 2 5 Calculation Parameters in the Wavelength Scan Method 2 4 2 2 6 Audit Trail in Wavelength Scan Method 2 4 2 3 The Report Screens in Wavelength Scan 2 4 2 3 1 The Overview Report Screen 2 4 2 3 2 Printing 2 4 2 3 3 Importing a report from the database 2 4 2 3 4 Filtering the report list 2 4 2 3 5 The Chart Report Screen 2 4 2 3 6 Chart Report Screen Functions 2 4 2 3 7 Audit Trail in Wavelength Scan Report 2 4 2 4 Calculations in Wavelength Scan 2 4 2 5 Peak Detection 2 4 2 6 Overlay 2 4 2 7 Joined Reports 2 4 2 8 Wavelength Scan Measurement Example eA TMe sir enc occ aoe sk es as 2 4 3 1 The Acquisition Screen in Time Drive 2 4 3 1 1 Run parameters 2 4 3 1 2 System Status 2 4 3 2 The Method in Time Drive 2 4 3 2 1 Acquisition parameters 2 4 3 2 2 _ Common Parameters 2 4 3 2 3 Calculation 2 4 3 2 4 Curve Parameters in the Time Drive Method 2 4 3 2 5 Calculation Parameters in the Time Drive Method 2 4 3 3 The Report Screens in Time Drive 2 4 3 3 1 The Overview Report Screen 2 4 3 3 2 Printing 2 4 3 3 3 Importing a report from the database 2 4 3 3 4 Filtering the report list 2 4 3 3 5 The Chart Report Screen 2 4 3 3 6 Chart Report Screen Functions 2 4 3 4 Calculations in Time Drive 2 4 3 5 Time Drive Measurement Example A e OF A
63. Case sensitive Here you can set search line and options as well Search from beginning to search text from the beginning of document Otherwise search will be performed from current page Case sensitive to distinguish letter cases lower case and capital types on searching On clicking OK text search is performed and the first found element is highlighted To continue search click F3 The following element will be highlighted UVS Software for Manual Version 2 20 Oct 2007 Page 162 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _ Deda Tes Gabit U 2 8 24 Report Export Report Designer allows exporting a built output report to different formats for further editing archiving sending via e mail etc To export you must add the desired FR export components to the Delphi form Export to 7 formats is supported They are PDF Excel XML RTF HTML text CSV There is the ability to send report via e mail in any above listed formats with Report Designer means gd EHAA A 110 Excel table XML Text File CS file HTML file RTF file Excel table OLE POF file Exports in Report Designer use one of the following three methods Layer by layer object transferring to resulting file is performed alternately Expert accuracy is approximated to original Table on object transferring transitional matrix of object allocation is used There is high accur
64. ER MANUAL _DeSoTes GmbH UY 3 221 6 samples 1 reference This mode allows measurement of 6 samples in the sample cell holder against 1 reference in position 1 of the reference cell holder Only the sample cell holder moves during measurement The figure below shows the cell holder positions at start and end of a measurement if all 6 cells are measured Start End Measurement Measurement Reference Beam _ _ _ Reference Beam i _ _ _ Sample Beam Sample Beam Prt Cal e lle oe oe E 851 UVS Software for Manual Version 2 20 Oct 2007 Page 185 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi TL CA ae 6 samples 6 references This mode allows measurement of 6 samples in the sample cell holder against 6 references in the reference cell holder Both holders move simultaneously during measurement The figure below shows the cell holder positions at start and end of a measurement if all 6 cells are measured Start r End RG Measurement i Measurement Reference Beam _ _ _ _ EE Reference Beam _ _ _ _ _ Sample Beam Sample Beam Po G a 5 5 UVS Software for Manual Version 2 20 Oct 2007 Page 186 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DesoTes GmbH UY a 2 0 10 samples 2 references This mod
65. Fix_1 4 29 08 2007 07 59 04 Probe Chrom Blindwert als Referenz 0 11 10 2007 14 32 01 Probe Chrom VI sequentiell 0 11 10 2007 14 27 23 Test_RC1_1 0 18 09 2007 16 25 33 Projekt Test_RC1_1 1 19 10 2007 14 21 57 Projekt Project Jf OK Q Cancel Enter the required file name in the input window select a project if available and click on the OK button 2 3 5 Loading existing data files Data which has already been acquired can be viewed via the main Report window in any of the measuring modes Ei Method A Report UVS Software for Manual Version 2 20 Oct 2007 Page 35 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi UY Select the Report window by clicking on the Report button The left hand section of the window contains a list of acquired data files which can be viewed and loaded by clicking on the appropriate line 2 3 6 Data conversion to EXCEL format Data files can be converted directly to Excel XLS format to be read from Excel spreadsheet programs Excel must be installed for this function to be used The data will be transferred directly to Excel and can then only be saved and processed within Excel Caution This data cannot be re imported into the UVS software and is not subject to any further controls by the UVS software with respect to security considerations for electronic documentation e Click on the icon to transfer data to Excel Excel i
66. HVal soe 9 Pi ee rx aria o gt BIU BAYMP E Ree R Code Data Page a ee eo ee a eo ek 961118 121138 148 16 1 188 17 t 18 19 208 21 8 220 238 2h 8 8 a a ed Data Variables Func gt FilterChange2 A E FilterChange3 FilterChange B FilterChangeS Paget o MasterData3 lal ChartObjectc 3 MasterDatal E StringGridobje 4 ha gt x Pagel TfrxReportPage vj m co mpany Ottice E JNamel Revision z a a pi f oO 3 T E Ei h Q a OG Properties Events b Cc a O N a P T A or a te BackPicture igned BackPicturePrintable True BackPictureVvisible True BottomMargin 1 Color Mi ciNone Columns 0 DataSet Not assig Duplex dmNone EndlessHeight C False Endless Width C False Font TFont Frame TfrxFram LargeDesignHeight C False LeftMargin 2 MirrorMargins Cl False Name Pagel Orientation poPortrait OutlineText PaperHeight 29 70 Papersize 44 PaperwWidth 21 PrintIFEmpty True PrintOnPreviousPac _ False ResetPageNumbers _ False RightMargin 1 Tag 0 TitleBeforeHeader M True TopMargin 1 visible True D D m v ih n v m m a a n 7 o m Qa 3 M Ge E E E ed E A Ed m e m a g a a TimeFrom B TimeTo UU E U feu nt ut amaaa oO Lal A E incl iv D i o Q lt wn or Qo tad Derivate 4 3 3 3 3 3 3 g o oO i Q o Fact
67. Header1 DATE L l PageFooter PageFooterl r 1 i Pager When running the report you will see that the objects in the finished report are allocated on the page in the appropriate position Thus bands are responsible for object positioning in required places Depending on band type we can add objects either at the top or at the bottom of the page on the first page or on the last one The basic bands which we would need in most reports work in the following way Page header band is displayed at the very top of each page Page footer band is displayed at the very bottom of each page Report title band is displayed at the top of the first page but below the Page header band depending upon the TitleBeforeHeader page property assigned in the object inspector UVS Software for Manual Version 2 20 Oct 2007 Page 149 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _ DeboTeo Gabit TU Report summary band is displayed at the very end of a report at white space 2 8 12 Picture object The next object to examine is the Picture object It is also frequently used in reports With the aid of this object you can insert a logo a photo of an employee or any other graphical information The object is able to display graphics in BMP JPEG ICO WMF and EMF formats Let s examine the capabilities of the object Create
68. L XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DeSoTes Ombi UY For text framing it is better to use text object embedded properties instead of single graphic objects lines rectangles etc try not to use background objects under transparent text objects Keeping these simple rules in mind will help you to create a report which will look perfect after export to any format using table or table based marking for data presentation Below there are some examples of correct and undesirable object arrangement on report design creation Bad Good Objects are displaced horizontally It is necessary to use alignment according to extension lines as far as possible for objects to have the same horizontal coordinate Memo 2 Good Objects are overlapping In such a case on export to table diagram format additional useless lines and columns and also 3 additional cells in crossing zone are created lt is recommended to get acquainted with demo reports included with the Report Designer installation for mastering basic methods of optimum report development UVS Software for Manual Version 2 20 Oct 2007 Page 173 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _ DeboTes Gabit U 2 9 Managing curves 2 9 1 Direct Printing of Curves Clicking on the Chart tab in the Report screens of modes such as Wavelength Scan gives the user a direct full screen display of the curves correspondin
69. Local zy Time Drive O Domain E Auto Rate ASSAY E Calibration Current password Doo T o gt Baseline Run L Options Pi T n Eh Login 11 36 31 2 2 2 Options Clicking on Options in the main display opens a dialogue window for entry of various parameters relating to reporting and archiving functions of the software UVS Software for Manual Version 2 20 Oct 2007 Page 12 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DesoTeo GmbH TL 2 2 2 1 General options The first window is the General window for definition of company name etc for inclusion on reports and also for the directory locations for the backup and database functions Mo ptions General Audit Trail Print Controls Extras Projects Instrument Type Serial no pilot amp Firmware Uy lamp off at program end C Set Uvikon parameter at method load C Com Ports Fi Uvikon COM d ption Company OTtice Dept Logo J delphib Projects Uw S900 bilderDusoTec Logol ESS bmp Directory Backup JdelphibProjects WV S900 Backup 2 Backup Archive J delphi6 ProjectsUVSS001UVSxDetabaset Export PATH J idelphibProjects WUY S900 Sx Database Database UVS Software for Manual Version 2 20 Oct 2007 Page 13 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi U Instrument The section contains data regarding the type of instrument serial numb
70. M sample 7 VM blank Where RW Result for wavelength W F Factor and VM represents the measured values obtained for sample and blank respectively The value RW is then used for the defined calculations for the individual wavelengths Recalculation after for example changing the factor value is possible at any time by clicking on the Calculator icon before the data has been saved UVS Software for Manual Version 2 20 Oct 2007 Page 99 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL eel UY 2 4 5 5 Wavelength Program Measurement Example Wavelength Program measurement of 4 samples using an optional automatic 6 6 cell changer Four wavelengths are used 400 500 600 and 700 nm The first sample is a Blank Define the method to be used mee f ea te 4 AAE Method E Remit nt ena de hed mialani i Palisa pete Eom cond imocss Maman Date 0602 2004 11 07 28 Click on the Acquisition tab in the upper left corner and select a method by clicking on the button next to the method window in the lower left of the screen Rechertest o Jamae 18 tf S000 2004 1084 UVS Software for Manual Version 2 20 Oct 2007 Page 100 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Dre a iY a mean Ea Po m Tn Ps aie ee T ET ii i iz Running
71. N 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DesTeo GmbH UY 3 3 Thermosystem Thermopack 3 3 1 Installation Connect the thermosystem or thermopack the serial line COM 1 Start the UVS Software the thermosystem thermopack will be detected os la Manual operation If the thermosystem thermopack is detected an additional window is shown in the control display area of the acquisition window Instrument Control wf Thermo System sample T Reference C OO set OO Set ethod Thermo System Method E Sample C Reference C Reference 00 OU Samples h eee i al Blank Value 0 0000 Enter the required sample or reference value and the press the Set button the thermosystem thermopack will then move to the required temperature value If a thermopack is detected only the sample is shown 3 4 Stirrer only UVSX 3 4 1 Installation Switch off the instrument and plug in the cable on the accessory board connector in the sample compartment Switch on the instrument and start the UVS software The stirrer will be recognised by the software during the initialisation process Access to the stirrer is possible in all modes using either direct control in the acquisition screen UVS Software for Manual Version 2 20 Oct 2007 Page 190 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL NS OT USER MANUAL ees hotometer Control Autozera Stirrer Wav
72. New template Created from Administrator Remark Date 20 10 2007 17 11 37 Last modification Choose the template you want to edit and double click on it A new window will be opened and shows the report designer UVS Software for Manual Version 2 20 Oct 2007 Page 127 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL NS AT USER MANUAL Keii ea FastReport new File Edit Report View Help B g A rey a 9 a Se amp A B F fal m e 100 O avi 0 gt BZ U YAY 222 MM u w a BJ Wavelengths E NoofResults axi i e 2 8 o gh a6 OF g Ot e e ee a gee 6 e a AP g Ag 20 o on a ee E A eee a ae y Report Data Variables Func Q T Data E iS MethodDataset A T O Paget E Method J d ba E Revision 7 B FixWavelength ies E Scanstart fE ScanEnd __ Pagel TfrxReportPage v NoOFWL pe Properties Events BackPicture AveragingTime lal BackPicturePrintabl V True E Delay A BackPictureVisible V True E LampChange a BottomMargin 0 a uk Strg s DeuteriumLamp Color W cione E TungstenLamp ER Columns 0 2 FE T FilterChange1 n Strg 9 m DataSet Not assigr py g B FilterChange2 Duplex dmNone E Filterchange3 OLE EndlessHeight C False a Paste Strg y E Filterchange EndlesswWidth C False E FilterChangeS EFont TFont i Pai Delete E Remark v EFrame
73. S 900Sourceogo roche bmp Directory Backup D Backup1 2 Backup D Backup Archive JDelphib Projects JY SS001UYS Deatahase Database PATH J Program Files U y Sx Database UVS Software for Manual Version 2 20 Oct 2007 Page 193 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL WS OAT USER MANUAL IUS Prepare method with liquid handler Activate the liquid handler in the method by ticking the checkbox If you want to add a new liquid handler method or link this method with a liquid handler method press the button right of the box Fixed Wavelength m gt a a efx C rite protect fz V Show only active methods METHOD REVISIONUSED Method not active Fi 226_Formettyp 01 E i Lamp change 230_Formettyp 01 poemen 233_Formeltyp 03 240_Formettyp 01 245_Formettyp 02 260_Formettyp 01 274_Formettyp 01 277 _Formettyp 01 280_Formeltyp 01 290_Formeltyp 01 1292_Formettyp 01 300_Formeltyp 01 312_Formettyp 01 316_Formettyp 02 _ 330_Formeltyp 02 334_Formettyp 01 334_Formeltyp 04 340_Formettyp 01 340_Formeltyp 02 340_Formettyp 03 340_Formettyp 06 340_Formeltyp 07 340_Formettyp 09 340_Formettyp 10 Acetat 340_Formettyp 11 _ 340_Formettyp 12 340_Formettyp 13 i Tm Tungsten Lamp Wavelength Len Deuterium Lamp Delay 00 Filter change 1 280 Averaging time 1 0 s Decimals 4 4 Abs T Abs Mj Filter change 4 Fitter change 2 Fitter change 3 7 t Repli
74. SOFTWARE USER MANUAL UVS Software for UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DeSoTes Ombi U Table of Contents 1 GENERAL INFORMATION 00 0 0 ccs cecnecesseensceenecnsneenseeeeesneneeneneenes 9 Lel Se ee E E oa a ent Pen ere Tone eee aut ne eT eT 9 tea Be Gh eee tone en ere NE een eee eee ee 9 1 3 INSTALLATION and REGISTRATION ere ee ee 9 2 OPERATING INSTRUCTIONS nnnnannnnnnnnnnnonononnnnnnnonenunnnnnnnnnnnnnnn 10 E IAT T N eee ee nen amen es tetra rer rete moRT ET ame y ene gems Stet eters renee et eee esse ae eee ee 10 oy ie VRS 28 eee eee ee ee noe ee ene eee nee enter eet ee eee eee 10 Kfar mee Uneaten ease eee rere eT Eee tet Tee e ee eer ee ein hye Te aeae teeter T ttre Cente meer TT Tete Merete nT T tte eet meee tee T 12 Eele ee UE ac scetiertene oi A 12 Be O e r E EEE TEESE A EA EIE A EEEE EE E E AE E A ETIE AE E A TETN 12 2 2 2 1 General options 13 After changing com ports please start UVS again 14 2 2 2 2 Audit Trail 15 2 2 2 3 Print Control options 16 2 2 2 4 Extras 17 2 2 2 5 Projects 18 Fe E TERENE 19 2 2 3 1 Backup 19 2 2 3 2 Restoring Backup Data 19 2 2 3 3 Archive 21 ddk Uor mangem rkr rE EE EREA EE EN 23 2 2 4 1 Password 24 2 2 4 1 1 User rights 24 2 2 4 1 2 Adding users 25 2 2 4 1 3 Login rights 25 2 2 4 1 4 Login blocked 25 2 2 4 1 5 Additional Rights 25 2 2 4 1 6 Saving data 25 2 2 4 1 7 Password expired 26 2 2 4 1 8 Passwo
75. Signature icon it is possible to see the complete details of the signature entries for the given report Select the report from the list and click on the icon to obtain the Signature information PA signature loj x acumen tame evisian signed Rev Fw Test 1 4 4 iqnature Date Full Mame Created 08 03 2003 10 07 25 Administrator f Approved 08 03 2003 10 26 55 Administrator Document Approval f Accepted 08 03 2008 10 28 56 Administrator Document Accepted 2 9 4 2 3 Signature Audit Trail The signature details are also recorded as part of the audit trail process and can be viewed in the report trail 210 I Bx FW Test 1 fe 08 03 2003 10 07 25 08 03 2003 10 07 32 3 08 03 2003 10 07 32 4 08 03 2003 10 28 55 5 08 03 2003 10 29 56 2 9 4 3 Deleting a Signed Report Created Report modified Report modified ApprovalSign AcceptSign Description Remark Derived from Revision 3 Description 5 Description 5 Document Approval Document Accepted For a given report it is only possible to have one signed report with the respective report name In rare cases it may be necessary to correct a signed report after signature and then re sign tt The UVS software allows a signed report to be deleted by users with administrator rights only and the deletion is recorded in a separate Deleted Reports Audit Trail A new report can be signed in the normal way as described ab
76. Specific Template Blocks General Template Blocks export Bela Print extended document List of some predefined report templates This will give a complete report which contains also details of the complete method used and the signature box for electronic signature confirmation Print small document This is a report which contains only the basic results of a measurement and some other limited information regarding measuring conditions etc Print table This report contains only the table of the measurement values and other data linked to the table UVS Software for UVIKON 900 XL XS Manual Version 2 20 Oct 2007 Page 131 of 202 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _ DeboTes Gabit UY 2 8 Report design editor The report component is supplied with an embedded designer which can be called in design time by double clicking on the TfrxReport component The designer provides the user with all the tools for designing a report s appearance along with the ability of simultaneous previewing Designer s interface meets up to date requirements It contains several toolbars which can be docked wherever you want 3 FastReport 93 fr3 File Edit Report View Help OBR 2am 4 OB 9 ey sea le Nostyle Taria Bz u RAYS Be WM Wh i E E S k fco bata raen D Ah 4 Te Wate 3 teste 6 fk 7 eB 8 elt Weim Aso 1S 1 bee 18 ie Bees AOAN TEAM EN PRE ELLIS a 3 Data Va Fu
77. TWARE for UVIKON 900 XS XL USER MANUAL _DesoTes Gabit UY The KeepAspectRatio option is enabled by default it stops the picture from distorting when the object s sizes are modified This option works only together with the Stretch option Therefore when applying any object dimensions a drawn circle will remain a circle without turning into an oval The stretched picture then occupies only the part of the internal space of the object needed to display it using correct ratios If the option is disabled a picture will be stretched to fill the object size and if object s size does not correspond to the initial dimensions of the picture it will be distorted Another useful property available in the Object inspector only FileLink You can put a filename c picture omp or variable containing a filename picture_file to this property The picture will be loaded from given filename when you run a report Note If the name of the picture object is Logo then the picture defined in the options was loaded 2 8 13 Chart object Mostly used in scan and kinetik methods shows this object the chart of the data Please select the chart by clicking the right mouse button R frxChartViewDialog Cancel 2 8 14 Stringgrid object The string grid object contains all numeric data shown as a grid table Please select the string grid in the combobox and set the other parameters The text in the grid can be formated
78. TfrxFrame E used 3 LargeDesignHeight _ False Select All Strg A E MeasureTime LeftMarain a E NoofReadings MirrorMargins _ False E AutoZeroatStarl Name Pagel y Orientation poPortrait i OutlineText PaperHeight 29 70 PaperSize At 3 E Reference H E ReferenceName SipperVol B SipperDelay B SipperActive TH Bring to Front A Send to Back PaperWidth 21 ReadingTime PrintIfEmpty M True Bj Samplertype PrintOnPreviousPac _ False E PeakMode ResetPageNumber _ False MinAbs RightMargin 0 E Treshold Tag 0 E TimeFrom TitleBeforeHeader M True E TimeTo TopMargin o E DelayToStart visible M True Derivate DerivateLevel Calculate Factor E AutoCalculate E ActivityActive B ActivityFactor E Mathfunction E ReferenceRev E Created E creationDate E LastChange K3 ji gt M Create field v Ci Create caption ackPicture he background page picture mo Sb kk Dee we A Ge Ae a 16 es As A a A a as a i 8 Centimeters 15 69 6 85 Pagel Edit the report options with clicking on the right mouse and select edit Define now the page options like size margins orientation Pape Options Other options Width cm Height 29 70 cm rientation a Fortrait Landscape Right Bottom First page Default Other pages Default wt UVS Software for Manual Version 2 20 Oct 2007 Page 128 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MAN
79. The Printer group you can select a printer via which you want to print a report set printer properties for example printing quality and choose print to file Printer Name i S Microsoft Office Document Image Writer ka where Print to File The Pages group you can select which pages to print all current selected range Pages all OC Current page Enter page numbers andfor page ranges separated by commas For example 1 3 5 12 The Copies group you can set how many copies to print If Collate flag is set and you choose to print several copies at first one report copy is printed then the next etc If flag is disabled several copies of the first page are printed then several copies of the second one etc Copies Collate The Other group UVS Software for Manual Version 2 20 Oct 2007 Page 119 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DeoTes GmbH UY Print you can select which pages to print Variants All pages Even pages Odd pages Order print pages in direct or reverse order from last to first page Duplex handle duplex by default report settings are used or choose one of duplex options vertical horizontal simplex Other The Print mode group you can select one of the printing modes Prink mode a gt a Default s Frink on sheet Default wt l gt a Default Default mode The printer prints on the sheet define
80. To display a derivative click on the Show derivative checkbox which will open the spinbox for selection of the required order Set the required derivative order in the spinbox and click on OK The curve display will automatically change to show the new derivative curve 2 5 1 2 Show cross points Selecting the Show cross points parameter will display each data point collected on the curve as shown in the zoomed example below m Series 0 m Series 1 O Series 2 V m Series 3 o Series 4 m Series 5 LaNa Smoothing A filter can be introduced in the data acquisition in order to reduce noise Smoothing can be selected with as an averaging or a Bezier function High smoothing will reduce noise however high smoothing will also reduce the amplitude of the peaks obtained and can also result in distortion of peak shape Average This is a rolling average function based on 3 calculated points Only Y values are calculated P x Xin 1 Xin Xn 3 UVS Software for Manual Version 2 20 Oct 2007 Page 105 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi UY Bezier A Bezier curve is an approximation to a curve which would pass through all the control points The method used here for defining such a curve approximation is called the Bezier Bernstein approximation Savitzky Golay The fundamental idea is to fit a different polynomial to the data surrounding each data point The
81. Tungsten Lamp Iv Wavelength 190 0 nm Deuterium Lamp JV Delay oo s Filter change 1 260 nm Averaging time oa S Fiter change 2 340 nm Decimals Filter change 3 470 nm Abs T Fitter change 4 540 nm Replicates Filter change 5 700 nm Individual Multiplier Divisor alculation J Math Function Exclude rows Statistic IV uality Control Target value 0 00 oncentration J7 Reference name Created from Date Remark Last modification from Date 09 51 08 User dmin 4 The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not Caution lf measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by using the button In order to do this it is first necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can be entered 2 4 1 3 1 Acquisition parameters Method not active Inactivate and thus hide the current Method by checking this box Method not active E UVS Software for Manual Version 2 20 Oct 2007 Page 45 of 202 UVIKON 900
82. XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Wavelength Delay time Averaging time Decimals Abs T Replicates Individual Multiplier Divisor 2 4 1 3 2 Yat DuSoTec GmbH T UY arameter Wavelength 190 0 nm Delay 00 s Averaging time 01 amp Decimals b pi Abs T abs Replicates i vy Individual Multiplier Divisor 7 Defines the wavelength to be used for the measurement The instrument will wait for the selected delay time after the MEASURE key is pressed to start the actual measurement Defines the time the instrument uses to collect data for calculation of a mean value for one reading A long averaging time is useful to reduce noise when measuring at high absorbance values Defines the amount of decimals for the measurement values Selection permits definition of the mode to be used The user can select between either absorbance or transmission Defines the number of acquisitions per sample to determine the resulting mean E g Replicates 0 only one acquisition per sample Replicates 1 two acquisitions per sample By checking this box a separate dilution factor multiplier and weight factor divisor can be defined for each sample Common Parameters The right hand panel contains common parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change Tungsten lamp Deuterium lam
83. a calculated result E g W280 W260 W250 1 2 W300 W350 Nested functions are possible too some of the calculation functions shown in the dialogue above are described in more detail in the section on Calculation within this document Functions defined above will be included and stored with the method and thus used for any acquisition executed utilising that method The wavelength range can be limited for the calculation of the maxima and minima Examples WL scan was executed in the wl range from 220 to 500 nm MIN 250 300 provides the lowest signal value within the WL range 250 to 300 nm even if the scan was executed from 220 to 500 nm MAX 250 300 provides the highest signal value within the WL range 250 to 300 nm even if the scan was executed from 220 to 500 nm MIN provides the lowest signal value over the entire scan range from 220 to 500 nm MAX provides the highest signal value over the entire scan range from 220 to 500 nm In addition the new functions MINL and MAXL indicate the related wavelength in a similar way Examples WL scan was executed in the wl range from 220 to 500 nm MINL 250 300 provides the wavelength for the lowest signal value within the WL range 250 to 300 nm even if the scan was executed from 220 to 500 nm MAXL 250 300 provides the wavelength for the highest signal value within the WL range 250 to 300 nm even if the scan was executed from 220 to 500 nm MINL provides the wavelength for the lowes
84. acy to original based on the assumption that rules of creating correct report sample were followed Report Design References chapter Enveloping all report objects enveloping is performed on page image There is full original accuracy It is used on export to graphic formats 2 8 25 Export to PDF Format PDF Portable Document Format a platform stand alone format of electronic documents created by Adobe Systems The free Acrobat Reader package is used for viewing This format is rather flexible it allows inclusion of necessary fonts vector and bitmapped images it allows transferring and storage of documents intended for viewing and further printing Export method is a layered one On exporting to PDF format the dialogue box for output file parameter settings appears UVS Software for Manual Version 2 20 Oct 2007 Page 163 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Yh AT USER MANUAL _DesoTes Gmbt UY Export to POF Page range C All C Current page C Pages Enter page numbers andior page ranges separated by commas For example 1 5 5 12 Export settings Compressed Print optimized Embedded Fonts Background Open after export Export parameters Compressed output file compressing It reduces file size but increases export time Embedded fonts all fonts used in report will be contained in the PDF output file for correct file displaying on comput
85. ample the curve obtained would appear so M E Series 0 min All curve calculation functions described above can also be used to modify curves used for the activity calculations For example it would be possible to subtract a pre determined Blank value from the curve and then multiply it by a different factor in this example the formula could be 2 S 0 2 This would give the following result M E Series 0 min UVS Software for UVIKON 900 XL XS Manual Version 2 20 Oct 2007 Page 109 of 202 UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTeo GmbH U An example for defining two activities would appear so 20 F 10000 Move bounds by cursor Oo 2 4 amp amp WW i 1 ot A wh SS Sw Ft yH Aaj i aa oe mim The bounds for activity calculation may be moved by the cursor with real time calculation Activities can be calculated with a mathematical function UVS Software for Manual Version 2 20 Oct 2007 Page 110 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DeSoTes Ombi UY 25 21 Activity Results The results of the activity calculation over the requested time period can be viewed by clicking on the Activity tab in the Report screen This displays the actual activity value for the sample plus the calculated regression line and also the correlation coefficient In addition the Table tab will show the actual activity at each point on the
86. an be docked wherever you want The report manager takes the contents of the templates and join it with data from the application adal Scan E DoR 3S Acquisition E Method IE Report Brr BR 4 Wwe nee ag C Arite protect Ez Select the print report type here Hex Special rer ort Date User IPROJECT D a 2 2 22 06 2006 LJ _ i iP ce eee ee a l a Hom 1 a2 0 2007 Jes o o o 16 f i fft 1 Bi Blank m2201 E FE E i nares Bf Holmium 2 2204 1 0 1910 2007 LJ as220 2 27 06 2006 admin SS e e a a l ae cis220 3 28 06 2006 admin Uy pray seat asa beet bee i bie a aa els240 1 27 06 2006 admin 12 erecta tia cis240 2 28 06 2006 admin EE aa Folie 3 0 20 09 2007 LJ Projekt A fle Peete _ Folie 3 0 20 09 2007 LJ Projekt amp Te Folie 3 1 20 09 2007 LJ Projekt A yo aita pje Met Rev Folie 6 0 20 09 2007 LJ Projekt A ne Sa ee tee CRinarSGoLaan al Folie 6 4 20 09 2007 LJ Projekt A l Holmium 0 20 09 2007 LJ Projekt A W BB art i 1 HHomium 1 20 09 2007 LJ Projekt A WES el eres et anon E a EEI aA Bd e Abs z Holmium 6 0 18 04 2006 admin 04 ee ae a er eee a panel 200 nmimin 1 08 06 201 Print Template Special v _ Holmium Q 18 04 200 Grid Sture Holmium 4 23 09 201 T h e ist of Holmium 2 b3 09 204 Holmium Fits o 4p 10 204 ae Nestle Folie 0 20 09 209 Nestle Folie 1 209 20
87. anel contains common parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength Tungsten lamp When checked the tungsten lamp will be switched on and used according to the setting for the lamp change Deuterium lamp When checked the deuterium lamp will be switched on and used according to the setting for the lamp change Filter change 1 5 This parameter defines the wavelength used to switch the filter Bandwidth Selects the spectral bandwidth of the spectrophotometer Cell changer Defines the operating mode of the cell changer if fitted UVS Software for Manual Version 2 20 Oct 2007 Page 84 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL _DeSoTes Ombi U 2 4 4 2 3 Calculation alculation Abs I 961 abs The calculation selection permits definition of the mode of calculation to be used The user can select between either absorbance or transmission 2 4 4 2 4 Wavelength Wavelength Humber of 3 Wavelengths WA Wavelength nm 1 Gale A maximum of six different wavelengths may be chosen The A of the wavelengths can be defined in the rows below 2 4 4 2 5 Curve Parameters i
88. arameters must be selected before starting a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Autozero at start of measurement gt Load previous saved data from a report UVS Software for Manual Version 2 20 Oct 2007 Page 81 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DesoTeo GmbH U 2 4 4 1 1 Run parameters The run parameters are entered in the Method panel and in the Photometer control panel Method p cao Set Samples Defines the number of samples to be analysed Checkbutton Press this button to accept the number of samples the sample grid will also created Autozero Defines whether an autozero should be executed before starting a measurement Method The method box contains the name of the method and directly adjacent the actual revision number of that method The method is selected from a separate window which is opened by clicking on the button adjacent to the revision box Hethods ia Method Revision Last Change A Aleschesrbest 130 07 2004 10 18 54 Tact 0 30 00 2004 10784 ngu O 30001 2008 10247 l Dde lbp urag C alphabelicaly This window contains a list of all available methods including all revisions of the method and the date of last modification Selection is made by highlighting
89. as left right center and automatic orientated With automatic orientated the text is left orientated if it is a alohanumerical text and right orientated if it is a numeric only value Expand to full width The grid was expanded to the whole width of the object otherwise it is only equal to the calculated size of the text Autosize Must be checked if expand to full width is checked Show title A column and row header wil be shown Must be enabled if show header is checked show header A header of he grid is visible UVS Software for Manual Version 2 20 Oct 2007 Page 151 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _ Deo Tes Gabit UY R frxStringGridDialog Z Z E spand to full width F Autosize Show Header F 2 8 15 Data splitting Let us pay attention to a peculiarity of this report There is a lot of blank space at bottom of the pages Why does it happen When a report is constructed the Report Designer core fills whitespace of the page with bands After displaying each band the current position shifts down When Report Designer finds out that there is not enough space to display the next band its height is larger than white space left on the page then a new page is formed and band displaying continues there This operation continues being performed as many times as there are records in the dataset Our report contains an object with large text and that is why the band height
90. at lij Opens the edit dialogue for formatting functions See the separate section of this document dealing with curve format for more details R 30 Converts chart format to a 3D display The icon functions as a toggle switch between a 2D and 3D display Click on this icon to control the depth of the 3D display using the mouse cursor Clicking on H this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Double function icon allowing zooming of the complete curve 08 Click on this icon to move the complete curve display using the mouse cursor Clicking on ooh this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Click on this icon to rotate the complete curve display using the mouse cursor Clicking on mn this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Clicking on this icon will return the function of the mouse cursor to normal thus allowing use of the normal zooming functions etc in 3D displays 2 4 3 4 Calculations in Time Drive The calculation modes for curves and activities are generally embedded in the method and are described above and in detail in the Calculation section UVS Software for Manual Version 2 20 Oct 2007 Page 77 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL errira UVY Recalculation after
91. ata which will be needed for comparison etc Therefore great care must be taken to select the ideal time to archive all data lt is also possible to selectively control archiving by using the file based backup program module to save data before deleting that data in UVS which does not need to be archived immediately The original data can then be restored as described above and the archived data removed The location of the archived database is pre defined in the options page in the UVS software and therefore the user need only define the actual year to be archived and then click on the Archive button A prompt appears requesting confirmation A Data from Year 2003 will be copied to archive and then deleted in origin database Click on OK to complete the archiving process UVS Software for Manual Version 2 20 Oct 2007 Page 21 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL DuSoTec GmbH Duss Software Teclek GmbH 1 Menu item Options General Options Setting the directories for Backup and Archive Archive Execution of Archiving in the Backup window of the main menu Prior to execution the year can be selected Re importing data from an Archive Selection is made in the Report menu of a Method e Selection of the year lists the corresponding data for the selected year e Data can be re calculated and partially combined only within a given year e In the Report a range of sea
92. ates and template blocks W Edit Template 1A Fix Wavelength n F Report Template O Wavelength Program Write protect E Method Template Specific Template Blocks O Sean General Template Blocks Template groups Templates O Timedrive Name Autorate Assay Calibration Overlay O Trail O General Kind of Templates Created from Luggi Jaufmann Remark Date 16 09 2007 17 03 32 Last modification Administrator from Date 02 10 2007 08 05 34 To edit and create new templates click on the tab Templates and uncheck Write protect Template groups Templates Write protect ZOO E H a a fo t To create a new template click on the Button on the task bar Then you are asked to give the template e name UVS Software for Manual Version 2 20 Oct 2007 Page 126 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL TRL Duss Software Tomik GmhH EEG Template groups Templates O Fix Wavelength Report Template O Wavelength Program C write protect Moa OM oS ers sO a Method Template Specific Template Blocks Scan General Template Blocks sees T EMERSIONE Autorate Assay gt aA _ Scan_Calculation_Graphics O Calibration Scan_PeakDetection_Calculation_Graphics Overlay Scan_PeakDetection_Graphics O Trail Scan_RawData Scan_SmallReport General
93. being edited The default white background of the designer and service windows can be modified by the Workspace and the Tool windows buttons The LCD grid color option increases contrast of the grid lines a little and it improves their visibility on LCD monitors UVS Software for Manual Version 2 20 Oct 2007 Page 138 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DesoTeo GmbH UY The Show editor after insert option controls what happens when new objects are inserted If the option is enabled its editor will be displayed each time an object is inserted When creating a large number of blank objects it is recommended to disable the option Disabling the Show band captions option you can disable bands captions in order to save some place in a page At that the band s captions would be written inside of it The Show drop down list of fields option stops the drop down list from displaying when pointing with the mouse to a Text object which is connected to data fields This may be necessary if there are many narrow text objects in a band The Free band placement option disables snapping of bands to the page This option is disabled by default and bands are automatically grouped in page according to their function A gap between bands can be set in the Gap between bands field 2 8 5 Report settings A window with report parameters is available
94. ber reports reports Checked security rights Visibility The permissions for individual users are modified by the administrator by checking the appropriate boxes UVS Software for Manual Version 2 20 Oct 2007 Page 24 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DeSoTes Ombi U 2 2 4 1 2 Adding users In order to add a user it is always necessary to unclick the write protect checkbox and the click on the or symbols to add or remove a user respectively Click on and a dialogue box will prompt for a new user name Please enter user domaingroup name e Local Domain y Select local or domain and enter a name and click on OK the new user will be entered in the list and the permissions for the new user can be selected using the dialogue boxes on the right of the window 2 2 4 1 3 Login rights Clicking on the drop down arrow cause the three possible user types to be displayed as a drop down list Select the required user type from the list 2 2 4 1 4 Login blocked Clicking on this checkbox can be used to prevent a user from accessing the system temporarily Permanent exclusion of a user should be made by deleting the user completely from the list using the button 2 2 4 1 5 Additional Rights Users can be assigned the right to delete reports If this checkbox is not ticked the user is not allowed to delete reports 2 2 4 1 6 Saving
95. blocking option The bottom object will always be displayed closely to the object which contains more text irrespective of text size in the upper objects 7 l B lee me i hi In this example if the Shift option is enabled for this object the bottom object will shift twice since it is located underneath two objects and thus an unnecessary gap is formed 2 8 18 Headers and footers of a data band Each data band may have header and footer Headers are output before a data band footers are output after all data records are output Here is an example of how the headers footers working in case of simple report UVS Software for Manual Version 2 20 Oct 2007 Page 156 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL UEN Header Header1 header header A data data data Footer Footer data footer data L al footer A simple example of title page creation Let us use our previous report with one data level Add a new page to it this will be the second page To move it to the front of the report grab the page tab using the mouse and then drag it to the place near the first page At that the pages order will be changed Switch to the new page and place a Text object with the Our report text inside in the middle of the page That is all we need to do The report with a title page is finished UVS Software for Manual Version 2 20 Oct 2007 Page 157 of 202 UVIKON 9
96. boring cells borders to adjoin each other It is important that cells do not cross and arrange in layers Export filter algorithm will cut off cells but export result may be far from desirable you will see not exactly what you wanted to Arrange objects in such a way that they are placed in line vertically as well as horizontally Guidelines can help to perform this UVS Software for Manual Version 2 20 Oct 2007 Page 171 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS USER MANUAL Bedell UY iz Using guidelines in the designer To use guidelines in Report Designer designer just click on the horizontal or vertical ruler limiting report page from the left and the top Then holding the mouse button down drag the guideline to the required position on the page You will be able to place objects immediately along guidelines horizontally and vertically Text objects grid alignment can also be helpful in case of cells overlapping Keep an eye on enabling grid alignment in designer options In order to simplify alignment you can extend grid pitch Setting of grid pitch and alignment can be found in designer menu View Options Grid Deviener Options fl m H lai to Grid F LOD grid cokor Girer r Show editor afer insert e Show droprechorwor Fields leet Aree bands placement Li JLo UVS Software for Manual Version 2 20 Oct 2007 Page 172 of 202 UVIKON 900 X
97. bsorbance 0 0 0 0000 580 0 Set oo The lower right section of the display contains the actual status of the system and displays the following parameters gt Current sample being measured gt Actual wavelength gt Actual absorbance value gt Temperature where applicable n addition the lower right panel also allows manual setting of wavelength in order to allow rapid monitoring of the unit 2 4 1 2 Parameters in Fixed Wavelength Acquisition Clicking on the parameter tab in the acquisition allows access to the following parameters Factor 1 0000 Vie f Abs ST Factor The factor can be used to calculate concentrations or other values if the response of the substance measured is known In this case use of a calibration file would not be necessary View These checkboxes permit toggling between absorbance and transmission values for the display of the acquired data The data is always saved according to the definitions in the method independent of this setting UVS Software for Manual Version 2 20 Oct 2007 Page 44 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL WS AT USER MANUAL Te 2 4 1 3 The Method in Fixed Wavelength Click on the Method button in the upper left corner to open the Method window E laix d E Method eral Report tel IV write protect JV Show only active methods Revision Used Method not active f Lamp change 190 nm arameter
98. cates ts A Filter change 5 Individual Multiplier Divisor d Calculation Response Time _Math Function 81 41 1 F Exclude rows Liquidhandler z 221 Units Statistics Sipper Quality Control Target value _ Calibration o eroertooooaooerftoesefteroeseteseteaet eet eaeeaearoeseo a Pee LW Pos 1 Created from deckerp_admin pate 29 06 2004 16 33 09 Femerk Last modification aaa admin Date 27 10 2005 12 41 25 i 12 44 13 User admin C write protect p LJ Rinsing station Sample 1 x Position 10 imm a f m of Z Sample Fill with y order Asoirate mm A a 10 Rinsing Statio See ined Fill with x order x Step mm 12 Rir Y Step mm i 12 No of Pos in X 3 No of Pos inY 8 Rack width mm 50 No of racks Select a method i Remark Created from admin Date 31 10 2005 12 49 56 Fan aa cnn Date 31 10 2005 1250 57 Y OK x Cancel UVS Software for Manual Version 2 20 Oct 2007 Page 194 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTeo GmbH U Create a new gilson method Press the button and fill the save name in the window Gilson method Sampler Rack Write protect Rack C Rinsing station Sample 1 Position w ZSample g an Fill with y order ror i Ta L J p fmm niai 1Y Position am
99. contains all parameters necessary for the execution of the measurement Numbers of samples to be measured Number of blank samples measured as the first samples Autozero at start of measurement Load previous saved data from a report YYYY WV UVS Software for Manual Version 2 20 Oct 2007 Page 41 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N DuSoTec GmbH USER MANUAL UY If Individual Multiplier Divisor is activated in the Method you may add a multiplication division factor before the measurement values Both multiplier and divisor have to be defined The factor not used should therefore be set to 1 E g if you choose the multiplier of 1 7 for a sample you have to set the respective divisor to 1 The calculation will be applied by clicking on the Calculate icon in the selection toolbar You may also define a description for the factor by clicking on the fixed row to enter description FP ced Wavelength R pps i a par bcqgition E iiia Ta Rapai riami Neb Sa D Diii geal ie cbc Civit Aia 1 She 2 Aes J Area al oe 4 F 118 i face iaa nF Fa 05 t DEI 0 Sot 0 foid 020m 025 12 i ey Jaa Oia j diia i5 Click on the fixed row to enter description onee Comoe Autoren WA Aes es ery a S300 Set Mahad oine _coleration F i Sampie i ai Eiai BA Sempies h 3 a a Bana vawe i ia Fiche Liner cache 2 4 1 1 1 Statistic If Calculation is activated in the
100. created as a language for scientific and technical documentation interchange Suitable for usage by people who are not specialists in nesting It is used for creating relatively simple but nicely designed documents Besides document structure simplification hypertext support is included into HTML Export method is a table diagram one On exporting to HTML format the dialogue box for output file parameter settings appears UVS Software for Manual Version 2 20 Oct 2007 Page 168 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Yh AT USER MANUAL _DedoTes Omit UY Export to HTML table Page range All OC Current page Enter page numbers andfor page ranges separated by commas For example 1 3 5 12 Export settings Styles Fixed width All in one Folder Multipage Page navigator Background Pictures JPEG Open after export Export parameters Styles transferring of text objects design styles Disabling increases exporting but worsens document appearance All in one folder all additional files are saved in the same folder with main file Page navigator special navigator for fast shift between pages is created Fixed width blocking of automatic table diagram width modifying on changing preview window size Multipage every page will be written to separate file Background export of graphic attributes assigned to report page Pictures includes gra
101. d The method is selected from a separate window which is opened by clicking on the button adjacent to the revision box methods I eee x Method Revision Last Changs J Fester gli 20 10 1854 Tati 300 S04 10784 neu O 30012008 102447 ner e by uge C alphabetically This window contains a list of all available methods including all revisions of the method and the date of last modification Selection is made by highlighting the required line and then clicking on OK The order of Methods may be displayed by usage or alphabetically A certain wavelength may be chosen manually and set immediately in order to allow rapid monitoring of the unit Manual Version 2 20 Oct 2007 Page 93 of 202 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Ss OU 2 4 5 1 2 System Status The lower right section of the display contains the actual status of the system and displays the following parameters gt Current sample being measured gt Actual wavelength gt Actual absorbance value gt Temperature where applicable 2 4 5 2 The Method in Wavelength Program Click on the Method button in the upper left corner to open the Method window WA wavelength program 031218_wiprogram1 0 1031218_ywiprogramt 1 0 040118_conc UVS Software for Manual Version 2 20 Oct 2007 Page 94 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Yh AT USER MANUAL _DeS
102. d in a report One preview page is printed on one sheet l gt Split big pages Split big pages This mode is useful if you need to print A3 report on A4 sheet One preview page is printed on several sheets If you choose this mode you have to choose the sheet size Print on sheet as well a l gt Join small pages Join small pages This mode is useful if you want to print A4 report on A3 sheet Two or more preview pages are printed on one sheet If you choose this mode you have to choose the sheet size Print on sheet as well gt i Scale Scale mode Report is printed on specified sheet All report output is scaled One preview page is printed on one sheet If you choose this mode you have to choose the sheet size Print on sheet as well After clicking on OK report printing begins If the Print to file flag is selected file name is called And report is saved to this file file with orn extension It contains a copy of information sent to printer UVS Software for Manual Version 2 20 Oct 2007 Page 120 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 REPORT MANAGER DuSoTec GmbH Duss Software Toctsik GmbH UY The report manager is supplied with an embedded designer which can be called in design time by double clicking on templates The designer provides the user with all the tools for designing a report s appearance It contains several toolbars which c
103. d the left maiie hiittan during mavind a moiise a frame UVS Software for Manual Version 2 20 Oct 2007 Page 133 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi UY appears As soon as you release the mouse button all the objects captured in the frame would be selected This operation can also be performed by clicking on the blank space on the page and moving the mouse cursor to the required position Alt left button If the Text object is selected it edits its contents in place 2 8 2 Toolbars 2 8 2 1 Designer mode bar The bar is integrated with the object bar and has the following buttons Ceon nme oesorpion lm Object selecting A standard mode of operation in which a cursor allows to select objects modify their sizes etc _tent Clicking on this icon allows dragging a report page Clicking on the left button doubles the zoom adds 100 while clicking the right one zooms out by 100 When holding the left mouse button while dragging the selected area would be zoomed Text editor Clicking on the Text object allows editing its contents right on the report page If you hold the left mouse button when moving the cursor the Text object appears in the selected place and then its editor launches Format copying The button becomes enabled when the Text object is selected When clicking on the Text object with the left button it copies f
104. d with the so called left and bottom axes Y and X respectively 2 x Axis Titles Legend Panel walls 3D Iv Wisible Scales Title Labels Ticks Minor Position w Behind re W Automatic fw Visible Inverted M Auto Change Maximum 1 545 M Auto Change Minirum 0 012 Change Desired Increment 0 001 Logarithmic LogBase 10 Eottom Asis Depth Asis UVS Software for Manual Version 2 20 Oct 2007 Page 175 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _ DedoTes Gabit UY The required axis to be formatted is selected by clicking in the list in the left hand panel Above this panel there is also a checkbox which allows the axes to be removed from the chart visible or not The significance of the other parameters is described briefly below 2 9 2 1 1 Scales Automatic The software uses dynamic scaling depending on the level of the signal obtained In many cases this can be confusing for the user and generally it is recommended that once known the range should be set manually for routine operation Visible Check this box if you want an axis to be visible uncheck to remove it Invert Checking this box will invert the chart Maximum lf automatic is set to off a fixed signal maximum can be defined here Minimum If automatic is set to off a fixed signal minimum can be defined here Desired increment The desired axis incremen
105. data Clicking on the Password required checkbox will activate a password dialogue in order to permit saving of the acquired data This security feature can be used to identify those users allowed to save data Only the currently logged in user provided with the appropriate rights can save data Clicking on the Overwrite allowed checkbox allows the user to also overwrite previously saved data Clicking on the Create additional report checkbox allows the user to save additional reports after creation of the initial report using the same acquired data UVS Software for Manual Version 2 20 Oct 2007 Page 25 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTeo GmbH U 2 2 4 1 7 Password expired A change of password can be forced at certain intervals as defined by this dialogue The option of never changing is also available but users should note that this option is not acceptable when operating in a controlled environment Entry of a time interval automatically produces a new box with the actual date by which the password must be changed 2 2 4 1 8 Password This allows definition of the user password for normal access control and also for saving of data if the checkbox described is activated The initial password for the new user must be entered in the appropriate box New users will be forced to change this password when they log onto the system for the first time This is a precau
106. dow 9 rulers When dragging a ruler to a report page the guide line which objects can be adhered to appears 10 status line UVS Software for Manual Version 2 20 Oct 2007 Page 132 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeoTes GmbH UY 2 8 1 Controls ADL l Key control Ctrl Z Edit Undo menu command Ctrl C Edit Copy menu command Ctrl V Edit Paste menu command Ctrl X Edit Cut menu command Ctrl A Edit Select all menu command Alt arrows The selected object is adhered to the nearest one in the specified direction 2 8 1 2 Mouse control Left button Selecting an object pasting a new object moving and resizing objects For the selected objects you can perform zooming in and out by dragging the red square in the left bottom corner of the selected objects group Right button Selected object s contextual menu Double click Object editor call Double clicking on the white space of a page calls the Page Settings dialogue box Shift left button Toggle object s selection Ctrl right button If you hold the left mouse button during moving a mouse a frame appears As soon as you release the mouse button all the objects captured in the frame would be selected This operation can also be performed by clicking on the blank space on the page and moving the mouse cursor to the required position Ctrl right button If vaii hal
107. down selection list which allows selection of the user who is executing the signature Only those users with the appropriate rights are displayed in the list i e for acceptance only users with acceptance rights will be displayed Select your user name from the list Full Name The full name is entered automatically once the user name has been selected as described above The full name is defined initially as part of the login definitions and cannot be changed here Password Entry of the user password is mandatory before any signature process can be successfully concluded Comment Here a comment relating to the signature can be entered and this will be stored with the other signature details and also shown on all printed reports Once a document has been signed off and accepted it is no longer possible to change that document therefore there are a series of prompts appearing to ensure that the user is aware of this process before actually proceeding The final prompt appears after the signee has confirmed that the document should be signed off and is the final safety check before closing the document Continue A You are going to accept the document The document will be locked and no modifications are possible Caution Only click on OK here if you are absolutely certain that the document in its current form is acceptable UVS Software for Manual Version 2 20 Oct 2007 Page 181 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900
108. e allows measurement of 10 samples 5 in the sample cell holder and 5 in the reference cell holder against 2 references gt Ref 1 in position 1 of the ref cell holder gt Ref 2 in position 6 of the sample cell holder Both holders move independently during measurement Start Beam End Measure Switching Measure Ref Sample i i ample Sample Ref Ref i lt lt lt lt Or OF ad Se Po f a Kate If more than 5 samples are selected in the 10 samples against 2 references mode the sample and reference beams are exchanged after the fifth sample has been measured This unique beam switching is possible due to the fact that both beams have a fully symmetric optical layout combined with symmetrical data acquisition electronics UVS Software for Manual Version 2 20 Oct 2007 Page 187 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DeSoTes Ombi UY 3 1 2 4 Manual operation only UVSX The cell changer can be moved manually using the Position spinbox in the Acquisition screen The sample position will be set automatically depending on the mode setting See above Select the required sample position and the press the Set button the cell changer will then move to the required position for measurement Instrument Control Cell Changer Photometer Control Mode sir v Autozero Wavelength nm gt Position 1 Se
109. e first sample A1 2 is the first measured value of sample no 2 F factor given with the acquisition window Other operators Sqr may be entered Exclude rows Enter the rows to be excluded The calculation of results mean std dev will not use these rows for calculation Target value This is a value for the statistics window The deviation will be calculated with the mean value and the target value Note Calculation and Concentration checkboxes cannot both be ticked at the same time An error message will be shown during saving of the method UVS Software for Manual Version 2 20 Oct 2007 Page 47 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _ DeboTes Gabit U 2 4 1 3 4 Concentration oncentration y Reference name es If the Concentration checkbox is ticked the system will use the defined calibration reference for the calculation of the concentration values based on the results of the measurement By clicking the button next to the Rev Box you can select the calibration file to be used The boxes show the Reference name of the selected file and its revision number Rev Note Calculation and Concentration checkboxes cannot both be ticked at the same time An error message will be shown during saving of the method 2 4 1 3 5 Audit Trail in Fixed Wavelength Method Pac pae Click on the Audit Trail icon to view the audit trail window for
110. eS Tuy yt Page breaks Continuous Page Header Footer Open after export x eee Export parameters Pictures enables graphic images export to output file Page breaks enables page breaks in RTF file WYSIWYG full compliance to report appearance Disabling the option allows optimization reducing the number of lines and columns in the output file Continuous generate continuous document without page breaks and page headers footers Page header footer header footer export mode Variants are Text h f exported as usual text Header Footer h f inserted in the document and None h f are not exported Open after export output file will be opened right after export via RTF files viewing program which must be installed in OS for example Microsoft WordPad Export peculiarities RichText objects are fully integrated into RIF format File appearance and size depend on report sample accuracy Report Design References chapter 2 8 27 Export to Excel Excel application for working with electronic worksheets It is included into Microsoft Office System Export method is a table diagram one On exporting to Excel format the dialogue box for output file parameter settings appears UVS Software for Manual Version 2 20 Oct 2007 Page 165 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DeSoTes GmbH UY Export to Excel Page range C All Cur
111. ear select cells Clear column Se 546 549 552 555 558 561 564 48 51 554 557 S60 563 S66 Yy OK x Cancel Note The number of samples must be equal or higher than the number of samples of the acquisitions otherwise the handler is not able to find any position Save the method with the post button UVS Software for Manual Version 2 20 Oct 2007 Page 199 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _ Dado Tes Gabit TL 3 Katalase Katalase is a module for calculation a time by a given absorbance start and end point in time drive if the module is installed A decreasing reaction will acquire in timedrive If the absorption has reached two defined points the time between is calculated The parameters must be entered in the Edit Calculation Parameter Checkbox Katalase should be activated Aktivity must be not activated From Absorbance value for start point To Absorbance value for end point Decimals Decimal points of the result Calculation Calulation formula Possible variables F individual factor per sample T calculated time from start to end point Example 13 17 F T 0 05 Time Interval The result will be marked with if the calculated time is outside Units Remark of the unit m Edit Calculation Parameter Curve function Smooth C Calculation Aans Si Show cross points C Average O Bezier
112. ection Scan_PeakDetectio Single Report Single Scan_PeakC ScanMethod Table Scan_RawData Template group Application select You can choose between different applications like Fix Wavelength Wavelength program Scan UVS Software for Manual Version 2 20 Oct 2007 Page 122 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL UY Database view Here you can select and add template groups To add a template group please uncheck Write protect on the top and push the button on the task bar To delete a template group push the button on the task bar Template group For inserting a template group at the selected cell push the right button under Report Templates To delete a template group click on the right button under Report Templates W Edit Template Fix Wavelength O Wavelength Program C Write protect me ee Scan peee Template groups Templates Name Method Template e ScanMethod Autorate Assay special Calibration O Overlay O Trail Report Templates Auto print Template 1 Template 2 Template 3 after acquisition Scan _PeakNetectin meee Select Template Extended Report Scar Check Uncheck as single Report Method Scan_PeakDetectio ScanMethod Peak Detection Scan_PeakDetectio Single Scan_PeakC ScanMethod Scan_RawData To add a new template please first select your template cell and now click
113. ed to a method and then define the appearance and properties of the curve such as colours overlay format scaling etc Functions defined here will be included and stored with the method and thus used for any acquisition executed utilising that method Formatting options using this function are described in a separate section in this document 2 4 3 2 5 Calculation Parameters in the Time Drive Method Click on this icon to open the calculation dialogue window for the method WMcdit Cakulation Parameter 101 xi Curve function Smooth Caloulaton Show dawala Mone Prot Show asipate C Amige c Denei i Peskdetection n Peak Valley 1 f Paak mim is 0000 Valley fF Activity from f D00 m Factor 1 0000 he OO mm Urats Actia 2 Reaction rom COO ma Fady 1 0000 be 0 00 mim Cakudaton F Het The calculation functions shown in the dialogue above are described in more detail in the section on Calculation within this document Functions defined above will be included and stored with the method and thus used for any acquisition executed utilising that method UVS Software for Manual Version 2 20 Oct 2007 Page 73 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL Beiler IUY 2 4 3 3 The Report Screens in Time Drive 2 4 3 3 1 The Overview Report Screen Click on the Report button in the upper left corner to open the Report window Pa Tire Drive AE
114. eed up the process and support the user a minimum number of entries need to be made The significance of the various fields in the dialogue above are described briefly below Document This box contains the name of the document to be signed together with its revision number This is entered automatically following selection of the report and the signature icon in the previous Report screen The Signed Rev box is used for later reference and contains the revision number of the signed report for a given document name UVS Software for Manual Version 2 20 Oct 2007 Page 180 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL Boeie IU Y Created The Created information is entered automatically from the audit trail for the selected report and is based on the time of last change and the currently logged in user who made the changes Approval The approval and acceptance areas are selectable using the check button immediately adjacent to the respective dialogues In order to approve a document ensure that the approval button is active All subsequent selections will be applied then to the approval process Acceptance The approval and acceptance areas are selectable using the check button immediately adjacent to the respective dialogues In order to accept a document ensure that the acceptance button is active All subsequent selections will be applied then to the acceptance process Login Name This is a drop
115. eek Detection Parameter Rent Tabie fe naume Control Audozenn Wavelength inm f seno Set ted bod aR er AEE aies Sampik Weneh eF rm Ahanta hte hod comet i i _ 580 0 0 0005 Samples z ET i User Gans UVS Software for Manual Version 2 20 Oct 2007 Page 51 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS aT USER MANUAL _DeSoTes Ombi UY This is the acquisition window from which all measurements are started In this window the following parameters must be selected before starting a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Autozero at start of measurement gt Load previous saved data from a report 2 4 2 1 1 Run parameters The run parameters are entered in the Method panel and in the Photometer control panel Method p wero circ Audoreng Samples Na al sao Sa Samples Defines the number of samples to be analysed Checkbutton Press this button to accept the number of samples the sample grid will also created Autozero Defines whether an autozero should be executed prior to the start of a measurement Method The method box contains the name of the method and directly adjacent the actual revision number of that method The method is selected from a separate window which opened by clicking on the but
116. eight in design mode option increases page s height several times more This feature can be useful if many bands are located in the page and must be used when working with the overlay band This only effects the page height in design mode 2 8 7 Creating reports 2 8 7 1 Report objects A blank report in Report Designer is presented as a paper page At any place on the page a user is able to add objects which can display different information such as text and or graphics as well as to define report s appearance Let us describe briefly the assignment of the Report Designer objects which are included in the standard package m c Band object allows creation on a design page of an area which has definite behaviour according to it s type A Text object displays one or several text lines within the rectangular area al Picture object displays a graphic file in BMP JPEG ICO WMF or EMF format Line object displays a horizontal or a vertical line 2 System text object displays service information date time page number etc as well as aggregate values zh Subreport object allows inserting an additional report design page inside the basic one ly the objects of Draw category represent different geometrical figures diagonal line rectangle rounded rectangle ellipse triangle and diamond lal Chart object displays data in the
117. elength nim 580 0 Set Move the track bar to set the stirrer speed the stirrer will follow immediately 3 5 Sipper In the Method screen you can define the way for sipper handling In addition to the volume and delay parameter the Rinse Air and Return 90 was added WM Fixed Wavelength a Acquisition E Method E Report m a gt gt i Write protect Show only active methods METHOD REVISION USED gt Method not active Lamp change Parameter m Tungsten Lamp Wavelength I 400 0 Deuterium Lamp l e Delay 0 Filter change 1 Averaging time 1 0 Decimals 4 MA Abs T Abs Replicates o eJ Individual Multiplier Divisor Fitter change 2 Fitter change 3 Fitter change 4 Fitter change 5 Bandwidth Calculation Response Time Math Function Liquidhandler To v Sipper Statistics Quality Control volume Target value Delay Rinse _ Calibration Air Reference name rey Return 90 fie Created from admin Remark Last modification from Date 18 09 2005 10 57 21 admin Date 18 09 2005 10 59 27 16 44 39 User admin After aqcuisition the system returns 90 if activated in the method UVS Software for UVIKON 900 XL XS Manual Version 2 20 Oct 2007 Page 191 of 202 UVS SOFTWARE for UVIKON
118. en objects are transferred from Report Designer report to necessary format This is carried out by special algorithms which takes object crossings into account and their optimal placing At object crossing points new columns and lines in the resulting output table appear That is necessary for saving of the Report Designer transferable objects exact positioning and for getting maximum resemblance between the result and original report A large number of cross UVS Software for Manual Version 2 20 Oct 2007 Page 170 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL Dado Tes Gabit UY objects in report design leads to an increased number of columns and lines in the resulting table This leads to the need to edit the resulting file in its own editor for further use Memo 1 For example on report design a slight crossing of two objects placed one under another on the same band The number of records on report forming was 150 On export to RTF format 450 lines will be created 150 lines for each object and 150 ones for crossing If we remove crossing there will be already 300 lines In large reports and on huge number of objects the difference will be really tremendous That of course will affect output file size 013 Objects in the report Export to Excel result Remember that during designing if you want to export your reports in any table format On creating tables in report keep an eye on neigh
119. eport list The report list in the left window will become very long with time and it may be necessary to filter the list so that only files of interest are shown to a user This is carried out using the Select Report icon rs Click on the Select Report icon and a dialogue window appears which allows selection of a view by date method user or name Select Acquisitions W Range from fi2092000 bo ji3032000 I Method 031218 calibration Method Revision _ Name Po User Po The appropriate checkboxes must be checked and where applicable either a date or name selected Clicking on OK will show only those reports which match with selected criteria shown in the list of available reports UVS Software for Manual Version 2 20 Oct 2007 Page 88 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _ DesoTes Gabit TL 2 4 4 3 5 The Chart Report Screen Clicking on the Chart tab will open a new screen containing the curve in a form which can be edited I ale x a z Acquistion Method EA Fagit E Faar E d i amp Fa E F Sampad EE npe 1 0 rnm E Sample 2 We anpe gt 220 nm Aidezend i Wavelength nm s0 Set Method Acquistion stale Mieg igih anthodesz p_e seeps ee J Rexinga 350 0 0 0516 Samples i2 i oo man Tina E zz Marss Frished ibe dmn Navigation arrows are available to move between the various reports c
120. er and firmware used In addition the accessories are listed and it is possible to enter also manually the serial number of the accessory UV lamp off If this box is checked the UV lamp will be switched off automatically at the end of the session Com Ports Define the com ports for uvikon only UVSX and thermo system if needed disable thermo system checkbox if no thermo system is connected After changing com ports please start UVS again Options These options define parameters which are included in the standard reports Under Logo the location of a company logo file can be defined which is included in every printed report Directory Here the location paths for the backup directories and database are defined Definition of a second backup location is not necessary but recommended UVS Software for Manual Version 2 20 Oct 2007 Page 14 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Yh aq USER MANUAL _DesoTeo GmbH TL 2 2 2 2 Audit Trail Mo ptions General Audit Trail Print Controls Extras Projects Audit Trail Error messages dl Report revision Method revision Mone All methods Only with used methods Trail Mone O Mormal trail Extended trail Error messages All displayed errors are stored in a text file if this option is checked Report revision lf this is checked a report cannot be changed All modifications are saved in a copy of the original report and
121. er processing Prints the curve as a single sheet report Formatting of the report is described in a separate section of this document dealing with curve format Opens the edit dialogue for formatting functions See the separate section of this document dealing with curve format for more details R 30 Converts chart format to a 3D display The icon functions as a toggle switch between a 2D and 3D display Click on this icon to control the depth of the 3D display using the mouse cursor Clicking on H this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Double function icon allowing zooming of the complete curve 08 Click on this icon to move the complete curve display using the mouse cursor Clicking on oo this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Click on this icon to rotate the complete curve display using the mouse cursor Clicking on mn this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Clicking on this icon will return the function of the mouse cursor to normal thus allowing use of the normal zooming functions etc in 3D displays 2 4 4 4 Calculations in Autorate Assay The calculation modes for curves and activities are generally embedded in the method and are described above and in detail in the Calculation section UVS Software for
122. ers where these fonts may be absent Output file size increases considerably Background export of graphic image assigned to a page into PDF file It considerably increases output file size Print optimized output of graphic images in high resolution for further correct printing This option enabling is necessary only when the document contains graphics and its printing is necessary It considerably increases output file size Outline option is enabled when report outline is used It enables export of the outline to the PDF document Open after export resulting file is opened right after export via PDF files viewing program which must be installed in OS by default for example Adobe Acrobat Reader Export peculiarities RichText objects are exported as a graphic 2 8 26 Export to RTF Format RTF Rich Text Format was developed by Microsoft as a standard for text documents interchange Now RTF documents are supported by many modern text editors and operating systems Export method is a table one On exporting to RTF format the dialogue box for output file parameter settings appears UVS Software for Manual Version 2 20 Oct 2007 Page 164 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTes Ombtt UY Export to RTF Page range All CO Current page Enter page numbers andfor page ranges separated by commas For example 1 3 5 12 Export settings Pictures Wt
123. es me I x Rinsing Station Z Sample Fill with y order uom Erm a Semele fram 100 1 Position c ample hose Imm E Pii Station Dispense mm L 40 Fill with x order Step mm i Z Rinsing Station Y Step mm fmm No of Pos inX No of Pos in Rack width mm No of racks Copy select cells Paste into cells Clear select cells Clear column Remark Created from admin Date 27 10 2005 12 40 13 s E acini Date 31 10 2005 1 251 18 rd OK x Cancel UVS Software for Manual Version 2 20 Oct 2007 Page 198 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL DuSoTec GmbH Due Software Tenik GmbH UY Set No of racks to 2 goto position 5 1 and paste the prior selected cells Gilson method Sampler Rack Rack Code Ja pRack fi 1 x Position ram 1 Position mm x Step mm Y Step mm No of Pos in No of Pos in Y Rack width mm No of racks Created from admin Last modification pen admin C write protect Rinsing station x Rinsing Station 400 Imm Y Rinsing Station 400 rar Z Rinsing Station Imm 100 Remark Date 27 10 2005 12 40 13 31 10 2005 12 51 18 Sample Z Sample Asoirate mm Z Sample Dispense mm Fill with y order Fill with x order iG24 Q CI Copy select cells Cl
124. esoTeo GmbH U Current sample being measured No of Readings Remaining Time also as progress bar Actual wavelength Actual absorbance value Temperature where applicable VVVVV WV 2 4 3 2 The Method in Time Drive Click on the Method button in the upper left corner to open the Method window 218 Ez o E Method E Report K a gt I IV write protect NM E Ez V Show only active methods 031217 _timedtrivet stolid z Lamp change 338 nm 031219 HTest_td_1 o pee aati TungstenLamp Wavelength 595 0 um Deuterium Lamp WM Delay 09 3 Filter change 1 280 nm Averaging time 25 Filter change 2 370 nm Measure time 50 m Fitter change 3 480 nm Bene 2 z e Fitter change 4 570 nm Autozero at stat V Fitter change 5 710 nm Auto Calculation J Calculation Abs T Abs v Created from famin Date fi 7 42 2003 15 46 19 panak a modification ann Date fira22003 1847 23 _ 4 gt 11 44 25 User dmin The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not Caution If measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by
125. esulting file will be opened right after exporting via default text files viewing program which is installed in OS Export peculiarities report design is not saved on transferring to this format graphic images are not supported exported page width is figured automatically depending on type of text objects on report page 2 8 32 Report Design References It is significant that the quality of the export into any other format depends greatly on competent design of initial report Report Designer allows a great number of ways to manipulate objects during report output creation This gives the advantage of fast development of any reports and their further printing Printed document will look just as on display And this is the primary intent of Report Designer report generator usage The downside of such development freedom is the complexity of exporting the Report Designer document to different data formats which have their own limits and requirements for information presentation and are sometimes rather complex In this chapter special design requirements of reports intended for export to other data formats will be discussed Many formats use table data presentation Formats such as HTML XLS XML RTF and CSV do Not allow cell crossing or arranging in layers when table marking this concerns HTML and RIF In contrast to freedom of report development in Report Designer designer Export filters as a rule take into account these requirements wh
126. evision control including an audit trail The method to be used can be selected from within the Acquisition page where also the naming and number of samples to be analysed can be defined For routine testing the various Methods to be used will normally have been pre defined and therefore it should not be necessary for users to access the Method screen All necessary entries for simple routine operation with a series of samples can therefore be made directly in the Acquisition area A result report can also be previewed and printed directly from the Acquisition area and is therefore not necessary for the user to switch between the various main windows The individual windows for the three main operating areas are accessible via main buttons in the top section of each window and are described below UVS Software for Manual Version 2 20 Oct 2007 Page 29 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DedoTes Ombtt TL Ea As The Acquisition Window The Acquisition page layout is similar for each mode and is shown below A graphical display is only available for those measuring modes where graphics are actually required and used e g Wavelength Scanning 4 Accuistion Ml Method Gj Report Working Area Selection bar E F2 stert 2x Standard tool bar T E AE T E V iSeries 0 Result display area i i i i i i i i i i
127. for a file name SAVE ACGUISHIOR Name Rev Date Project A Armoniumreihe 11 10 07 oo 11 10 2007 11 28 02 LJ_Test_Fix_1 4 29 08 2007 07 59 04 Probe Chrom VI Blindwert als Referenz 0 11 10 2007 14 32 01 Probe Chrom Vl sequentiell 0 11 10 2007 14 27 23 Test_RC1_1 0 18 09 2007 16 25 33 Projekt Test_RC1_1 1 19 10 2007 14 21 57 Projekt Project Jf OK Q Cancel Select the project if availabe enter the file name and click on OK UVS Software for Manual Version 2 20 Oct 2007 Page 68 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Ss fe d 2 4 3 Time Drive 2 4 3 1 The Acquisition Screen in Time Drive Select Time Drive from the main menu to obtain the following display eta This is the acquisition window from which all measurements are started In this window the following parameters must be selected before starting a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Autozero at start of measurement gt Load previous saved data from a report 2 4 3 1 1 Run parameters The run parameters are entered in the Method panel and in the Photometer control panel UVS Software for Manual Version 2 20 Oct 2007 Page 69 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL Beila UY
128. for example changing a Peak Detection parameter is possible at any time by clicking on the Calculator icon followed by selection of the parameter within the dialogue window 2 4 3 5 Time Drive Measurement Example Measurement of a sample Select the method to be used and set the number of samples to 1 Start the measurement by pressing the Start button and you will be prompted to insert the sample and click on OK to start the measurement Insert the sample in the correct position and click on OK The unit will now start to measure The final windows will look like this The curve display is plotted during the acquisition and can be used to monitor the progress of the measurement If the auto calculation box has been checked the regression line for the Activity calculation will be drawn immediately upon completion of the run E laj xi EL tion E Method Report E Abort x o a e c 8o o 35 0 4 2 3 min Chart Activity Peak Detection Parameter Method Instrument Control Cell Changer Acquisition status Values Method fro Test 0 fea Autozero Mode esr gt Sample HZ Wavelength nm Abs Samol f aj Position i aj Set Readings 180 550 0 0 4020 a Remaining o0 min aie 5500 Set oo i ESS 21 08 19 Finished User admin Various tab functions in the acquisition screen allow quick overviews of the results in table format as shown below act
129. ftware for Manual Version 2 20 Oct 2007 Page 30 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL Kanodia UY The list for selecting methods may be sorted alphabetically or by the access counts The most used method is on the top 1101 200 1314 T3120 1055 25 322125005 Sr 017 12 SOS 13 S56 119123003 131256 018122003 10 See 21d 2 as Wien 1 13 01 2008 1305 013122002 11 11 57 23122003 11 11 40 0 17 12203 14146 018122303 eS DaT kal aron The sample description can be imported from a text file from the explorer via drag and drop Select the text file in the explorer and drag it with left mouse button pressed to the description grid Then release the mouse button the text is dropped into the grid WR Fixed Wavelength a 2 Acquistion E Method E F2 Stat E sion E Report Sample 2 53 Sample 3 S4 Sample 4 S5 Sample 5 Datei Bearbeiten Ansicht Favoriten Extras Ea eed fa A Suchen E Ordner E4 verlauf g R X A Ez Adresse C Programme iti v Wechseln zu Dateiname Grobe Typ Desktop sample 1KB Textdatei aA Eigene Dateien ope Arbeitsplatz els 3 4 Diskette A Eb Lokaler Datentr ger C Description sample Textdatei Ge ndert 26 09 2004 17 55 E Dokumente und Einstellun 2 Program Files Gr e 48 Byte Attribute Normal UVS S
130. g a measurement gt Method to be used for the acquisition The method contains all parameters necessary for the execution of the measurement gt Numbers of samples to be measured gt Number of blank samples measured as the first samples gt Autozero at start of measurement UVS Software for Manual Version 2 20 Oct 2007 Page 92 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL DuSoTec GmbH Dussp Software Tomik GmbH 2 4 5 1 1 Run parameters The run parameters are entered in the Method panel and in the Photometer control panel Method p Method I 5 Phefemeter Carte eee Blank m aj Viavekreth Crim Samples z saoo Sa Samples Checkbutton Blank Samples Method Wavelength UVS Software for UVIKON 900 XL XS Defines the number of samples to be analysed Press this button to accept the number of samples the sample grid will also created Defines the number of blank samples to be measured blank samples are always expected at the start of a run The value of the measured blank is subtracted from the actual measured value of the sample prior to calculation of concentration etc If more than one blank is defined then the system calculates the average blank value for the blanks and then subtracts this from the actual measured sample values The method box contains the name of the method and directly adjacent the actual revision number of that metho
131. g to the selected report The appearance of the curve may be modified using the Edit function which is described in a separate section below Prints the curve as a single sheet report Click on this icon to obtain the formatting window below This method will only print the curve itself p TeeChart Print Preview OP x Printer Defaut Printer Setup Print Orientation C Portrait Landscape Reset Menge W View Margins eee cae J Proportional el esl ea ec ae The upper bar in this dialogue box controls the actual operation and selection of the printer parameters using the standard printer installation windows The left hand section of the window contains formatting functions which directly influence the print out itself e g margins detail etc Changes to these parameters directly influence the appearance of the print out preview in the dialogue window These functions are explained briefly below Orientation Selection of either landscape horizontal or portrait vertical format for the print out Margins Spin selection boxes allow direct setting of the individual page margins UVS Software for Manual Version 2 20 Oct 2007 Page 174 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DeoTes Ombi UY Reset Margins Changing the preset margins automatically activates the Reset Margins button click on this at any time to reset all margins to their default
132. ge 338 NM 1 Tungsten Lam v ey O O l EE gstenLamp M robert 0 Deuterium Lamp Iv a Lower 300 __ robert1 0 Wavelength 300 Filter change 1 280 nm robert a5 fe 7 Upper 420 nm Fitter change 2 370 nm _jtest 0 Wavelength test 200 0 Fiter change 3 480 nm Scan interval fo gt nm Fiter change 4 570 R Scan speed 500 nimmin Fitter change 5 710 nm Autozero at start D Auto Calculation i Calculation Abs T Abs x Created from aamin Date f18 12 2003 14 17 27 pema ssa admin Date f 9 12 2003 12 52 13 4 gt 11 33 30 User admin The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not Caution If measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by using the button In order to do this it is first necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can then be entered 2 4 2 2 1 Acquisition parameters Method not active Inactivate and thus hide the current Method by checking this box Lower Wavelength Def
133. ge 67 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DeSoTes Ombi UY Click on OK to start the next acquisition run or on Cancel to return to the original data set Printing Click on the Print icon to obtain a preview of the printed report which will always contain all measurements If the result is acceptable the report can be printed by clicking on the Print icon on the preview page The function Add peak detection to document includes as well the peak detection list before the graphics printout even for small document selection When the function Add peak detection to document has been selected and a calculation formula is defined then the calculation is printed on top and the peak detection list follows as second object before the graphic element Print Document with Single Curve If several scans were included in one graphic curve then it was sometimes difficult to identify the curves and all related elements The new print option Print Document with Single Curve allows to automatically printing each curve separately one sheet per curve or per curve pair if individual reference curves were selected The peak detection list and the calculations list contains as well only the values for the current single curve the next page contains then the next curve with the related peak detection and calculation results etc Saving Click on the Save icon and the system will prompt
134. gsten halogen lamp is used for all wavelengths above the lamp change wavelength Tungsten lamp When checked the tungsten lamp will be switched on and used according to the setting for the lamp change Deuterium lamp When checked the deuterium lamp will be switched on and used according to the setting for the lamp change Filter change 1 5 This parameter defines the wavelength used to switch the filter Bandwidth Selects the spectral bandwidth of the spectrophotometer Cell changer Defines the operating mode of the cell changer if fitted UVS Software for Manual Version 2 20 Oct 2007 Page 95 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL DuSoTes Ombi U 2 4 5 2 3 Wavelength Program calculations The right section of the method screen allows definition of the wavelengths to be used and also the calculation modes umber of i al calculations Calculation Number of i al r Wavelengths Wavelength gnm 1 EmN The required number of both calculations and wavelengths to be used must first be defined using the two spin boxes at the top of each entry window This definition will open the appropriate number of lines where the actual values can be entered directly Calculation Wavelengths are defined for calculation purposes as W n or w n respectively where n corresponds to actual number for a given wavelength as shown in the lower window In the example above the foll
135. he calibration and is traceable The Calibration procedure is best explained using an example In this example we will use a blank and two standards The calibration can also be made using the cell changer PSS Creating a Calibration File Select the Calibration mode by clicking on the Calibration icon in the main screen and insert the blank and the two standards in the cell changer at positions 1 2 and 3 UVS Software for Manual Version 2 20 Oct 2007 Page 111 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL el IUY Enter the number of samples 3 the required method a description of each sample and the concentration in the main Acquisition screen Clicking on the Parameter tab will allow entry of the units to be used for the result and also definition of an additional multiplier factor which will be used to multiply the value obtained to give the result The final screen will look like this BP Calibration Fixwavelength a td a Piss Click on Start to start the acquisition Once completed the system will automatically prompt for the type of calibration required Two possibilities are available using either a linear or quadratic regression UVS Software for Manual Version 2 20 Oct 2007 Page 112 of 202 UVIKON 900 XL XS The Calibration parameter dialogue box can be recalled at any time by clicking on the calculation icon Click the Linear checkbo
136. ich allows selection of a H view by date method user or name UVS Software for Manual Version 2 20 Oct 2007 Page 49 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _ DeSoTes Oabl UY Select Acquisitions E X W Range from fiz092000 to fi3092000 gt J Method 031218 calibration Method Revision Name Ps User The appropriate checkbox must be checked and where applicable either a date or name selected Clicking on OK will cause only those reports which satisfy all selected criteria to be shown in the list of available reports 2 4 1 4 4 Audit Trail in Fixed Wavelength Report Click on the Audit Trail icon to view the audit trail window for the report 2 4 1 5 Calculations in Fixed Wavelength Fixed Wavelength contains four basic calculation methods for final result determination gt Linear regression gt Quadratic regression gt Blank gt Factor In Fixed Wavelength the Blank and Factor calculations are executed automatically without the need to create a calibration file Blank The measured values for sample blank is subtracted from the measured value of each sample measured Factor The actual value obtained from the measurement is always multiplied by the factor parameter This is accessible by clicking on the Parameter tab in the acquisition screen The actual calculation is based on the following formula R F VM sample _ VM blank Where R Resul
137. ieeiaens 190 oe ee cS 116 EEE re Terry reentry ree rere sree nee Terre Tree 190 lt UVS Software for Manual Version 2 20 Oct 2007 Page 7 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL oaeiai UY ee FR ore erence chin bona tunnubia uasdaden sie waeb aban E E EEEE EEEE E 191 3 9 Liguid Nandiei sisssiiiniinsiiinisseiriprisseiininstadinna inaina arak irni eatin Eni n Ekibi NE saeksainbsanies 193 s a E ee eee eee as 200 UVS Software for Manual Version 2 20 Oct 2007 Page 8 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeoTes Ombi UY 1 GENERAL INFORMATION The user should be before using the instrument have read and understood this operating manual particularly the general warnings given below 1 1 GENERAL WARNINGS gt Always disconnect the mains plug before starting any work inside the instrument gt Any maintenance operation inside the instrument including lamp replacement must be carried out by a service engineer or an authorized person The user is normally only permitted access to the inside of the sample compartment gt Keep the sample compartment clean gt Set wavelength to zero during transport to protect the drive mechanism 1 2 CD CONTENT The CD ROM supplied includes UVS Spectroscopy Software Installation program UVS User Manual software User Manual in pdf format Adobe Acrobat Reader Installation programs UVS
138. iew window click on the chart tab to obtain the curve display Click on the Activate Cursor icon A vertical cursor can now be moved across the curve graph and the values for the wavelength are continuously displayed on the cursor line The arrow used to move the cursor is also used for the position of the Y axis and values for the current Y position are continuously updated in the legend window adjacent to the curve Additionally by clicking on the right mouse button you may add new comments edit and delete comments UVS Software for Manual Version 2 20 Oct 2007 Page 38 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL _DesoTeo GmbH UY si al TE ain BB Method Es Report trl as zs 4 v H 4 b ki A vela protect kal A ane 2 2 Beh i k EEE o 22 42 2008 08 35 05 pate 462 ieee f J 4 710 DD a a 1 mm Ack T i p 4 o p as r BS DOI rah Edt Commer i eam f 7 foo 3 e Comment i i f i m S0 0000 4 aian ai nl oe 20 4M At HD A A ees Ban BO GMI TT Te m0 CO EEEE Leer imin When the cursor is activated you may manually pick peaks by clicking the right mouse button and choose Peak Picking You can also activate or deactivate samples in the legend 2 3 13 Zooming Zooming is available for all methods where graphical curve displays e g Wavelength Scan Time Drive or Auto Rate Assay are available and is used to magnify a sectio
139. ill be switched on and used according to the setting for the lamp change Deuterium lamp When checked the deuterium lamp will be switched on and used according to the setting for the lamp change Filter change 1 5 This parameter defines the wavelength used to switch the filter Bandwidth Selects the spectral bandwidth of the spectrophotometer Cell changer Defines the operating mode of the cell changer if fitted 2 4 2 2 3 Calculation alculation Abar toT abs The calculation selection permits definition of the mode of calculation to be used The user can select between either absorbance or transmission 2 4 2 2 4 Curve Parameters in the Wavelength Scan Method Ee Opens an edit dialogue for defining curve parameters and related formatting In Wavelength Scan the curve parameters can be linked to a method and then define the appearance and properties of the curve such as colours overlay format scaling etc Functions defined here will be included and stored with the method and thus used for any acquisition executed utilising that method UVS Software for Manual Version 2 20 Oct 2007 Page 55 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTes Ombi UY Formatting options using this function are described in a separate section of this document 2 4 2 2 5 Calculation Parameters in the Wavelength Scan Method Click on this icon to open the calculation dialogue window for the method
140. in the form of either header or footer text is required This can be done by selecting the required function using the drop down menu and entering the text in the lower panel Avis Titles Legend Panel walls 3D Tite Style Position Format Text Gradient Shadow R Visible Adjust Frame lignment C Left Center Right Text WAVELENGTH SCAN DOCUMENTATION Alignment top or bottom is dependent on this selection while the horizontal position can be controlled directly from the Alignment box A custom alignment using coordinates is also possible by clicking on the Position tab The remaining tabs are used to define the font type colours frame and certain special effects such as shadowing etc 29 2 3 Legend The legend box is used to show which curve colour corresponds to a particular sample The default text used is Series N where N is the number of the sample in the batch This default text is only used when no sample description is defined otherwise the actual description will be used automatically for the legend UVS Software for Manual Version 2 20 Oct 2007 Page 177 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL _DeSoTes Ombi IU 2 x Axis Titles Legend Panel wals 3D Style Position Symbols Format Text Gradient Shadow W Visible Legend Style Automatic Inverted Test Style Left Value M Check boxes Vert Spacing 0
141. ines the lower wavelength for the wavelength range to be used for the measurement Upper Wavelength Defines the upper wavelength for the wavelength range to be used for the measurement Scan Interval This parameter also called sampling interval in other instruments defines the wavelength step between two measured data points UVS Software for Manual Version 2 20 Oct 2007 Page 54 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL IU Y Scan Speed Defines the wavelength drive speed to be used for the measurement Scanning speeds can be selected between 5 and 5000 nm min If you choose dynamic scan speed the instrument will automatically optimise the scan speed to suit the slope of your sample Autozero If checked an autozero will be made for each sample beforestarting the scan Auto Calculation If checked the calculation for peak detection smoothing etc starts automatically after acquisition using the defined parameters 2 4 2 2 2 Common Parameters The right hand panel contains common parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength Tungsten lamp When checked the tungsten lamp w
142. ivity etc UVS Software for Manual Version 2 20 Oct 2007 Page 78 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Cela IUY lel x E O Acquistion E Method E Report Dl start E Abort x SB 0 13083 0 01988 0 13099 0 00958 0 13123 0 01437 0 13149 0 01566 013174 0 01497 0 13200 0 01557 013218 0 01084 0 13239 0 01257 0 13261 0 01317 0 13282 0 01265 0 13309 0 01617 0 13324 0 00898 0 13343 0 01145 0 13367 0 01437 0 13396 0 01737 0 13419 0 01386 013436 0 01018 0 13449 0 00778 0 13475 0 01566 0 13502 0 01617 a E Feseeesseseeeeseeee Method Instrument Control Cell Changer Acquisition status Values Method frotet oo Ez Autozero _ Mode str x Sample HE Wavelength nm Abs Position f Set Readings 180 550 0 0 3661 Samples f EE oo min Time 550 0 Set 0 0 S E Note If you have programmed more than one sample you will be prompted for the next sample after each sample has been measured The final appearance of the screen remains unchanged If you repeat the measurement you will always measure the selected number of samples 1 in our example until you change the Samples parameter The system will prompt if the previously run sample data has not been saved before starting a new run A Data not saved Continue anyway Click on OK to start the next acquisition run or on Cancel to return to the original data set Pri
143. l disappear and the software will prompt for user name and password for log in UVS Software for Manual Version 2 20 Oct 2007 Page 28 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTes Ombi U 2 2 8 Exiting the system software You can exit the system software by clicking either on the Exit icon in the top left corner or on the X in the top right corner 2 3 OPERATION 2 3 1 General guidelines AA Acquisition Calculate Display The UVIKON uses an Acquisition Calculate Display scheme as shown below Acquire data for Calculate Display results of 1 to 10 samples using 1 to 10 samples using 1 to 10 samples using method parameters method parameters method parameters The system is structured to use individual measurement modes e g Fixed Wavelength which must be pre selected by the user The selection of the mode to be used is made in the opening screen which can be accessed immediately after the software has started and login has been completed This screen can also be reached at any time by exiting from the current mode in use ris a Ea Recommended Operating Practise Each measuring mode uses Acquisition parameters which must be pre defined in a Method The Method also contains the data necessary for the final display of the results in the form of a Report The Method is the key to maintain a trace to all results and together with the different reports is subject to a strict r
144. les handling of expression In this case Report Designer allows you to use another set of symbols to designate the expression The ExpressionDelimiters property which is equal to by default is responsible for it In this case the user can use angular brackets for the expressions instead of square ones a 1 lt myVar gt The lt gt value must be set in the ExpressionDelimiters property AS you can see the comma divides opening and closing symbols There is one limitation however the opening and closing symbols cannot be similar so will not work One can set several symbols for example lt gt Thus our example will look as follows a i lt myVar gt 2 8 11 Bands in Report Designer Bands are used for logically placing the objects it contains at a location on the output page When placing an object in a band such as Page Header we tell the report engine that the given object must be displayed at the top of each page of a finished report Similarly the Page Footer band is displayed at the bottom of each page together with all the objects it contains Let us demonstrate it with an example We ll create a report which contains the Hello text at the top of the page a current date to the right of it and a page number at the foot of the page to the right Open the Report Designer designer and click the New report button in the toolbar You will see a re
145. lo World text will appear This report can be printed out saved to a file fp3 or exported to one of the supported export formats 2 8 8 The Text object The Text object has many features Now we already know that it allows you to display text a frame and fill color The text can be displayed using any font of any size and style All the parameters can be set visually with the help of the toolbars gt No style F Arial io B TU FAY Z m llil fhill Here are some examples of text design UVS Software for Manual Version 2 20 Oct 2007 Page 144 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N AT USER MANUAL ULL Test C Test Test 7 1 Now let s look at other features of this basic object As an example let us create a new text object and put two lines into it This is a very very very long text line And this is another line the shorter one Let us enable the object frame and then resize the object up to 9x3 cm with the help of the mouse We see that the object can display not only a single line of text but several lines as well Now let us reduce the object width up to 5cm It is obvious that long lines did not find room in the object and therefore were wrapped This happens due to the WordWrap object property If it is disabled either in inspector or in the object context menu the long lines will be simply cut off Now let s check how the text alignment inside the
146. m Bottom cm Faper Source First page Default ki Other pages Default ka On the first dialogue page you can select size and alignment of paper as well as set margins In Paper source drop down lists you can select a printer tray for the first page and the rest of the report pages Page Options Other options Columns Number o width oo cm Positions Other C Print to previous page C Mirror margins Endless page width Endless page height Large height in design mode Duplex Default Ww UVS Software for Manual Version 2 20 Oct 2007 Page 142 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi UY On the second page you can set the number of columns for multi column reports printing The current settings are displayed in the designer The Print to previous page flag allows you to print pages beginning from blank space of the previous page This option can be used in case when a report template consists of several pages or when printing batch composite reports The Mirror margins option switches right and left margins of page for even pages during previewing or printing a report The Endless page width amp height option increases page s sizes depending on number of data records on the page when running a report In this case you will see one big page in the preview window instead of several standard size pages The Large h
147. mmediately above the curve display In Chart mode a range of new functions is available to the user for manipulating the presentation and properties of the current curve Similar to the function Peak Picking in WL Scan the new function Time Picking allows selecting the time using the hand symbol With a right mouse click the absorbance values can be selected at the cursor time position The manually selected list can be included into the document by selection the print option Add peak detection to document In this case a list including all selected values is inserted above the graphics 2 4 3 3 6 Chart Report Screen Functions A graphics toolbar is available within the Chart report screen UVS Software for Manual Version 2 20 Oct 2007 Page 76 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS A USER MANUAL DuSoTeo Ombi U EA om 5 oE op Ml a The use of the icons in the graphics toolbar allow direct manual manipulation of the currently displayed chart The function of the individual icons is described briefly below Shows position of values on X and Y axis and allows to manually pick peaks and to add edit or delete comments Opens the calculation dialogue window e Exports a curve to the Windows clipboard for further processing Prints the curve as a single sheet report Formatting of the report is described in a separate section of this document dealing with curve form
148. mplete Time Drive is carried out on the first sample before the cell changer moves to the second position and measurement of sample 2 is carried out This process continues until the selected No of samples has been measured Jihda Wavelength Program All samples are measured at the first wavelength then the wavelength is changed to the second one and all samples are measured again This process will be repeated until all wavelengths for all samples have been measured UVS Software for Manual Version 2 20 Oct 2007 Page 188 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DesoTeo GmbH U 3 2 Maintenance 3 2 1 Service Only available in UVS900 version This is a service function for checking the optical system 3 2 2 Thermal sensor calibration Define here the calibration curve if a thermo sensor is installed If not please let all cells blank Set a number of nominal values and verify your thermo sensor with a calibrated thermometer Example Let your temperature goto 30 C press Apply and fill the correct temperature of the thermometer into the nominal value Repeat this for 4 or 5 other temperatures and save it Now your thermo sensor is calibrated via the curve against the nominal values of the calibrated thermometer m Thermal sensor calibration A Ek males 22 24 26 Mominal Mo of tems al UVS Software for Manual Version 2 20 Oct 2007 Page 189 of 202 UVIKO
149. mplex The following examples demonstrate how these tags can be used text lt b gt bold text lt b gt lt i gt text in italic lt i gt lt b gt lt iI gt bold and in italic lt b gt lt i gt E mc lt sup gt 2 lt sup gt A lt sub gt 1 lt sub gt B lt sup gt 2 lt sup gt UVS Software for Manual Version 2 20 Oct 2007 Page 146 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DeSoTes Ombi U this is a usual text lt font color red gt and this is a red one lt font gt this is a usual text lt font color FF8030 gt and this is an orange one lt font gt text bold text text in italic bold and in italic E me Ay E this is a usual text andthis is a red one this is a usual text and this is an orange one L l 2 8 10 Displaying expressions with the help of the Text object One of the most important features of this universal object is it s ability to display not only a static text but expressions as well Expressions can be located in the object together with text Let us examine a simple example of how it can be performed Put the following line into the object Hello World Today is DATE Thus when running the report we can get something like follows Hello World Today is 01 01 2004 What lead to such result During Report Designer report building if an expression enclosed in square brackets is encountered the engine calculates it s value and inserts the value
150. n of the graph Simply move the cursor on the graph to the upper left corner of the rectangle to be magnified press the left button of the mouse and move it to the right while keeping the left button pressed A rectangle will be shown on the screen indicating the area that will be magnified When you reach the lower right corner of your area release the button and the area selected is zoomed to fill the graph area You can repeat the zooming function up to the maximum resolution by repeating the selection of the zoomed area To unzoom simply position the cursor on the graph press the left mouse key move the cursor to the left and release the left mouse key or double click with left mouse key UVS Software for Manual Version 2 20 Oct 2007 Page 39 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DedoTes Gabit TL 2 3 14 Adding comments to curves You can add a comment at any point on a curve for documentation purposes When a curve is displayed on the screen click the Activate Cursor icon in the Graphics toolbar move the mouse cursor to the point on the curve where the comment should be added and click the right mouse button A small dialogue box appears offering the choice of adding editing or deleting a comment Click on a selection and the appropriate dialogue window will appear ais Edit Comment Ioj xj Curve Series 0 Comment No O Input the desired comment text using alphan
151. n the Auto Rate Assay Method bE Opens an edit dialogue for defining curve parameters and related formatting In Auto Rate Assay the curve parameters can be linked to a method and then define the appearance and properties of the curve such as colours overlay format scaling etc Functions defined here will be included and stored with the method and thus used for any acquisition executed utilising that method Formatting options using this function are described in a separate section in this document UVS Software for Manual Version 2 20 Oct 2007 Page 85 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL TOS 2 4 4 2 6 Calculation Parameters in the Auto Rate Assay Method E Click on this icon to open the calculation dialogue window for the method MRE Calculation Parameter Haa amp The calculation functions shown in the dialogue above are described in more detail in the section on Calculation within this document Functions defined above will be included and stored with the method and thus used for any acquisition executed utilising that method UVS Software for Manual Version 2 20 Oct 2007 Page 86 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL IUY 2 4 4 3 The Report Screens in Autorate Assay 2 4 4 3 1 The Overview Report Screen Click on the Report button in the upper left corner to open the Report window LT PTE 5 scwdetion EE menoa
152. nded values of the absorbance UVS Software for Manual Version 2 20 Oct 2007 Page 16 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Yh AT USER MANUAL _DesoTeo GmbH TL 2 2 2 4 Extras m Options General Audit Trail Print Controls Extras Projects Acquisition Auto save after acquisition Auto print after acquisition L Contirm name at saving C Calculation Rights t Allow to modify calculation in kinetic acquisition Application Copy method and user database at program start al Convert methods trom UYS900 database C Archive data at program end Autosave If this option is checked then the data will be saved automatically after acquisition Autoprint Please select for printing immediately after acquisition Note See also the no preview option UVS Software for Manual Version 2 20 Oct 2007 Page 17 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _ DeboTes Gabit U 2 2 2 5 Projects akk Options Exit General Audit Trail Print Controls Extras Projects Project Mame H Scan Project Define here your projects and link it with the methods Fix wavelength wavelength program scan The name of the project is saved with the report UVS Software for Manual Version 2 20 Oct 2007 Page 18 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DedoTeo GmbH TL 2 2 3 Backup This function
153. nherit from base report 103 fr3 UVS Software for Manual Version 2 20 Oct 2007 Page 140 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS aT USER MANUAL _DesoTes GmbH UY You can learn about inheritance later in the Report inheritance chapter In this dialog you can see the base report s name if report is inherited detach the base report in this case your report will be standalone non inherited and inherit the report from one of base reports Controls in the third page of the dialogue allows you to set report s description properties Report Settings General Inheritance Description Description Name Author Description This report demonstrates the ability to draw diagrams on the Data bab Picture Browse Version Major Minor Release Build Created 0 09 2006 22 15 43 Modified 1 09 2006 18 27 19 All fields in this dialog are for information purposes only 2 8 6 Page options Page s parameters are available via either the File Page settings menu or by double clicking on page blank space The dialogue has two pages UVS Software for Manual Version 2 20 Oct 2007 Page 141 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _ DeboTes Gabit UY Page Options Other options Width cm Height 20 70 cm Orientation Portrait Landscape Margins Left cm Right cm Top c
154. nter Next page Transfer to next report page Transfer to the last report page alee Close window Close preview window 2 8 20 Control keys Smooth document scrolling PageUp Up down scrolling PageDown Ctrl PageUp Next previous page scrolling PageDown UVS Software for Manual Version 2 20 Oct 2007 Page 159 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS al USER MANUAL _DusoTes Gm UY Document beginning 2 8 21 Mouse control Action eseription Left button Click on selected object in interactive report report scrolling in hand mode move mouse with pressed button zoom in is performed in magnifier mode Right button Context menu in magnifier mode zoom out is performed Double click lt full screen mode it performs returning to normal conditions Mouse scroll Report list scrolling 2 8 22 Report printing To print a report click on 3 button or Ctrl P hotkey The window appears it is printing dialogue Printer Mame Sy Microsoft Office Document Image Writer w Where Print to file Pages Copies oa Number of copes ft O Current ooo C Pages Collate Enter page a andior page ranges separated by commas For example 1 3 5 12 Other Prink mode i z gt l gt l Default kra Order Direct 1 9 Duplex Default w Print on sheet Default v Let s look at options available in this dialogue UVS Software for Manual Versi
155. nting Click on the Print icon to obtain a preview of the printed report which will always contain all measurements If the result is acceptable the report can be printed by clicking on the Print icon on the preview page UVS Software for Manual Version 2 20 Oct 2007 Page 79 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL NS AT USER MANUAL Renal IUY Saving Click on the Save icon and the system will prompt for a file name Save CQU SHOR Name mmoniumreihe 11 10 07 0 11 10 2007 11 28 02 LJ_Test_Fix_1 4 29 08 2007 07 59 04 Probe Chrom Vl Blindwert als Referenz 0 11 10 2007 14 32 01 Probe Chrom Vl sequentiell 0 11 10 2007 14 27 23 Test_RC1_1 0 18 09 2007 16 25 33 Projekt Test_RC1_1 1 19 10 2007 14 21 57 Projekt Name ID Project Jf OK Cancel Enter the file name and click on OK UVS Software for Manual Version 2 20 Oct 2007 Page 80 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL se rat USER MANUAL Ss IUY 2 4 4 Auto Rate Assay The Autorate Assay mode is only possible in conjunction with an automatic cell changer Operation is basically identical with that of Time Drive 2 4 4 1 The Acquisition Screen in Autorate Assay Select Autorate Assay from the main menu to obtain the following display E Auto Rabe Assar ss This is the acquisition window from which all measurements are started In this window the following p
156. o of racks Remark Created from admin Date 27 10 2005 12 40 13 E irin Date 31 10 2005 12 51 18 Y OK x Cancel Set No of pos in x to 4 positions and select an area of positions by pressing shift and left mouse button on position 1 1 Then move to position 3 11 with holding down shift and left mouse button and release the mouse button at the position 3 11 The selected area is marked as blue except the first and the last position UVS Software for Manual Version 2 20 Oct 2007 Page 197 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL WS AT USER MANUAL Remon UY Gilson method Sampler Rack r v C Write protect R Code a Rack Rinsing station Sample z I 1 Position x Rinsing Station 4 Z Sample Fill with y order Ura m L J E Sabe mm J 1 Y Position i m ample ill wi mm p a Station Dispense mm Fill with x order Step mm i L Z Rinsing Station ann Y Step mm mm No of Pos in x No of Pos in Y Rack width mm No of racks Created from admin Date 27 10 2005 12 40 13 ios ee irin Date 31 10 2005 12 51 18 A OK x Cancel Copy the selected cells by pressing the right mouse button Gilson method Sampler Rack r v x C Write protect Rack Code S Rack Rinsing station Sample 7 1 x Position Sy e
157. oTes Ombi UY The left hand window contains a list of all existing methods including revisions and an indication of whether they have been used already or not Caution lf measurements have been used the method will be locked and can no longer be changed However they can be used for later measurements or modified with revision control Such methods are indicated by a in the used column list A new method can be created from within the method section by using the button In order to do this it is necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can then be entered 2 4 5 2 1 Acquisition parameters Delay time The instrument will wait for the selected delay time after the MEASURE key is pressed to start the actual measurement Averaging time Defines the time the instrument uses to collect data for calculation of a mean value for one reading A long averaging time is useful to reduce noise when measuring at high absorbance values 2 4 5 2 2 Common parameters The right hand panel contains common and calculations parameters which are required for every system and user method They will be stored and recalled along with all other parameters Lamp change This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tun
158. of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DusoTec GmbH UY sca lei a Si Acoution BB verea E Repor Gd 2 d ET r l k Aei 5 D o amp sie ty osn20 tes o os 02 2004 10 1200 Curves may be displayed as overlays individual curves can be removed from the overlay if required by clicking the checkboxes shown as part of the legend on the right of the curve Navigation arrows are available to move between the various reports contained in the list without the need to return to the Overview screen The name of the currently displayed curve set including the revision is shown in the box display immediately above the curve display In Chart mode a range of new functions is available to the user for manipulating the presentation and properties of the current curve 2 4 2 3 6 Chart Report Screen Functions A graphics toolbar is available within the Chart report screen e260 K oma The use of the icons in the graphics toolbar allow direct manual manipulation of the currently displayed chart The function of the individual icons is described briefly below Shows position of values on X and Y axis and allows to manually pick peaks and to add e edit or delete comments UVS Software for Manual Version 2 20 Oct 2007 Page 60 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL aa 1K ae m in loe Yat DuSoTec GmbH Due Sof
159. of a data DAN cccceccceccseccecceeeeeeeeeeeeeeeeseecauecsueeseeeaeesaaes 156 2 8 19 Report Viewing Printing and OXPOM cccceccccsececeeeeeeseeecseeeseeeessueeseeeesseeeseeeeenees 158 2 8 20 Control BOS yn sstvsenriussncntininanaenenioehshutieninaichiacdecdabtbubebenvkxhtonkebeinaentsahebeabnaesenanneaunueeben 159 a ee aie ears EE E eset a ree een mete eae 160 Coa PIG DUI a E 160 Dae Wi et E E E E E E E ETE 162 e PO E A E EA EE i 163 ame Wake EORI OF E E E EEEE 163 re re i eit E 164 e eTA E e ert gcse scenes AEEA E AE Chen EAE A eterno 165 ee Mt O EF T e orcnsstoaserarenterespare aco marsasomie we iommussoentenieeoasesoneaene 166 A WLS Me p 491316 is gre a Bl ae dif See ne enero ee ee ee ee eee ee eee 167 UVS Software for gt Manual Version2 20 Oct 2007 Page 6 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL UY Be E E A EEEE 168 E ix EARO TO TON Olt e a AR AR 169 2 8 32 Report Yt a re vinsee es ect nsewie ns enews 170 re eee eee ee eee nee een 174 2 9 1 Direct Printing Of Curves ccccceccceecceceeeeeeeeeeseeeeseeeeeaeeeeseeeesseeeeseueessaeeeseeeetaaeeesees 174 da2 SC and Formaning oi CE i csantcescecnimaorennitangssnenantanniaanpenepnasenninmaamenatiannioaeres 175 2 9 2 1 Changing the scaling Axis 175 2 9 2 1 1 Scales 176 2 9 2 1 2 Decimal Format 176 2 9 2 1 3 Title 177 2 9 2 2 Titles 177 2 9 2 3 Legend 177 2 9 2 3 1 Style 178 ins Oe E a 178 2 9
160. oftware for Manual Version 2 20 Oct 2007 Page 31 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DeSoTes Ombi U 2 3 1 4 The Method Window The Method page layout is similar for each mode and is shown below Graphical display functions are again only available in those measuring modes where graphics are actually used e g Wavelength Scanning Other functions such as calibration definition are also not present in every window Wisco la x E 2 Acquisition method E Report Working Area Selection Bar A e write protect X E Selection Toolbar Lower 350 nm Wavelength Jn Lamp change 338 nm Upper 750 nm Tungsten Lamp v Wavelength Deuterium Lamp D Scaninterval f nm Fitter change 1 280 nm A Filter change 2 370 nm Scanspeed f 000 v niminin Fitter change 3 480 nm Autozero at stat V Filter change 4 570 nm Auto Calculation D Fiter change 5 710 nm alculation Abs T Abs Bandwidth 2 nm gt Existing Method List E O O J Run and Calculation Parameters g Created from famin e 22 02 2003 17 17 36 e 2 ast netston paa oe saa Current Mathod History 17 36 30 User admin Working Area Selection Buttons for selection of working window e g Method Selection Toolbar Contains buttons for navigating the current method list plus buttons to allow addition or deletion of methods Existing Method List Contains a li
161. on 2 20 Oct 2007 Page 160 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DeSoTes Ombi UY The Printer group you can select a printer via which you want to print a report set printer properties for example printing quality and choose print to file Printer Name Sy Microsoft Office Document Image Writer w where Print to file The Pages group you can select which pages to print all current selected range Pages all CO Current page Enter page numbers andfor page ranges separated by commas For example 1 3 5 12 The Copies group you can set how many copies to print If Collate flag is set and you choose to print several copies at first one report copy is printed then the next etc If flag is disabled several copies of the first page are printed then several copies of the second one etc Copies gt Number of copies Collate The Other group Print you can select which pages to print Variants All pages Even pages Odd pages Order print pages in direct or reverse order from last to first page Duplex handle duplex by default report settings are used or choose one of duplex options vertical horizontal simplex Other The Print mode group you can select one of the printing modes Prink mode a gt a Default Frink on sheet Default wt UVS Software for Manual Version 2 20 Oct 2007 Page 161 of 202 UVIKON 900 XL XS
162. ontained in the list without the need to return to the Overview screen The name of the currently displayed curve set including the revision is shown in the box display immediately above the curve display In Chart mode a range of new functions is available to the user for manipulating the presentation and properties of the current curve Similar to the function Peak Picking in WL Scan the new function Time Picking allows selecting the time using the hand symbol With a right mouse click the absorbance values can be selected at the cursor time position The manually selected list can be included into the document by selection the print option Add peak detection to document In this case a list including all selected values is inserted above the graphics 2 4 4 3 6 Chart Report Screen Functions A graphics toolbar is available within the Chart report screen ER eR EET Le The use of the icons in the graphics toolbar allow direct manual manipulation of the currently displayed chart The function of the individual icons is described briefly below UVS Software for Manual Version 2 20 Oct 2007 Page 89 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DeoTes GmbH U Shows position of values on X and Y axis and allows to manually pick peaks and to add edit or delete comments Opens the calculation dialogue window Em F Exports a curve to the Windows clipboard for furth
163. ontains the current reports or Deleted Reports which contain audit trails for all deleted reports UVS Software for Manual Version 2 20 Oct 2007 Page 178 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL WS OT USER MANUAL TOS It is possible to print a report of the highlighted trail by clicking on the print icon in the top menu bar or alternatively the trail can be exported directly to Excel if required 2 9 3 1 Report Trail rr 01x 3s FW Test 1 fe No Date Time Username Acton o iem ovane NowVabe Comment 08 03 2003 10 07 25 admin Created Derived from Revision 3 Ea 08 03 2003 10 07 32 admin Report modified Description Description 5 es 09 03 2003 10 07 32 admin Report modified Remark Description 5 08 03 2003 10 28 55 admin ApprovalSign Document Approval ea 08 03 2003 10 29 56 admin AcceptSign Document Accepted 2 9 3 2 Deleted Reports The deleted report trail screen is obtained by clicking on the deleted report selection lolx Bx Deleted Report __ Report Name Revision UserName gt Nitrate scan without calc 1 admin No Dae tme UserName an em Oat Newe Comment E 08 03 2003 17 38 44 i Created Derived from Revision 0 2 08 03 2003 17 38 45 i Report modified Detect False 3 08 03 2003 17 38 46 i Report modified MinAbs 0 08 03 2003 17 38 46 i Report modified Treshold 0 5 08 03 2003 17 39 32 i ApprovalSign Approval to Test Delete 6 08 03 2003 17 40 04 i
164. or autoCalculate E ActivityActive B ActivityFactor z o ao RS oF S g 25 o amp aS oa lt w Oo a o so S E w a oe T Tamana E Activity2active DIT 3 o th o 3 E Time2To E Activity2Factor El Actcale E KataBsi T z ar faa t nN m rad gt 4 oO a M ackPicture he background page picture Create field y Ll Create caption F Centimeters 1415 70 12 39 i 12601 26 6 24123 1221 A 201 19 1 18 1 17 ae A 13 IZ 1 1 91 g a Gr Ft er Zr zed Add all the blocks onto the sheet you want to and click the save button for save and exit UVS Software for Manual Version 2 20 Oct 2007 Page 130 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL To import or export your templates please click on the following buttons the top F Edit Template Fix Wavelength A Te Template groups Templates O Wavelength Program _ write protect Cc Scan O Timedrive Autorate Assay Calibration Overlay Trail _ Scan_RawData Scan_Calculation_Graphics Scan_PeakDetection_Graphics Scan_SmallReport General s s Scan_PeakDetection_Calculation_Graphics Created from Administrator Date 20 10 2007 17 11 37 Last modification from l Date DuSoTec GmbH mee Tomik GmbH Duss Softw d in the task bar on Report Template Method Template
165. or Report screens Click on the Calculator icon to obtain the appropriate dialogue box and then click on the Activity checkbox UVS Software for Manual Version 2 20 Oct 2007 Page 107 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL Redladiepill IUY PP edit Calculation Parameter E Ho 5 Cure ureter Src jv Caku Shoe dame B 1 fF Hone ChatFunctionf Shoe Cote poms Average i Bea Wavelength Ha 1 Fureton T C Peakdetection A Peak abay L Zz in F Acity hon C00 mn Fato 10000 hp 1 0 mn Unig e Acta 2 feston Ma TEU in Factor 10051 bo SD mn Caloulstion 1 42 7 Hdp It is possible to define the section of the curve over which the calculation is to be made a factor and the units for the Activity value lt is now possible to set directly the following parameters From Defines the lower starting point on the curve time scale for the range of the Activity calculation to Defines the upper starting point on the curve time scale for the range of the Activity calculation Factor Activity is calculated by multiplying the slope of the regression line Absorbance change per minute by the factor F Units Defines the units to used for reports etc e g Abs min umol min UVS Software for Manual Version 2 20 Oct 2007 Page 108 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL DuSoTec GmbH Duss Software Teclek GmbH Using the above ex
166. ormatting which has the previously selected Text object into the object 2 8 2 2 Standard toolbar DO d ds ia teon Name f Description Creates a new blank report Open report Opens an existing report from the file Hotkeys combination Ctrl O T Save report Saves a report into the file Hotkeys combination Ctrl S Preview Performs report constructing and its previewing Pr Hotkeys combination Ctrl P UVS Software for Manual Version 2 20 Oct 2007 Page 134 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeoTes Ombi EI Adds a new page into the report Delete page Deletes the current page Page properties Calls a dialogue with page properties Calls the report variable s editor Cut Cuts the selected objects into the clipboard Hotkeys combination Ctrl X Copies the selected objects into the clipboard Hotkeys combination Ctrl C New dialogue form Adds a new dialogue form into the report Pastes objects from the clipboard Hotkeys combination Ctrl V Undo the last operation Hotkeys combination Ctrl Z Repeat Redo the last cancelled operation Hotkeys combination Ctrl Y Show grid Shows the grid on the page Grid pitch can be set in designer options Grid alignment During dragging or during modifying object sizes the coordinates sizes are modified step wise according to grid
167. our PC RichText objects are transferred as simple text graphic images transference is supported 2 8 28 Export to XML Format XML Extensible Markup Language is an expansible marking language XML is intended for structured data storage and also for information interchange between different programs Report Designer uses XML format for data transferring into Excel table diagram editor ver 2003 and later Export method is a table diagram one On exporting to XML format the dialogue box for output file parameter settings appears UVS Software for Manual Version 2 20 Oct 2007 Page 166 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DesoTes Ombtt UY Export to Excel Page range All CO Current page Enter page numbers and or page ranges separated by commas For example 1 3 5 12 Export settings Continuous WYSIWYG Page breaks Background Open Excel after export Export parameters Continuous generate continuous document without page breaks and page headers footers Page breaks includes page breaks in resulting document WYSIWYG full compliance to report appearance Disabling allows reducing the number of lines and columns in resulting table Background export of filling color assigned to report page Open Excel after export resulting file will be opened right after exporting into Excel Export peculiarities Excel program must be installed on yo
168. ove UVS Software for UVIKON 900 XL XS Manual Version 2 20 Oct 2007 Page 183 of 202 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes GmbH UY 3 OPTIONS AND ACCESSORIES 3 1 AUTOMATIC CELL CHANGER 3 1 1 Installation Switch off the instrument and plug in the cable on the accessory board connector in the sample compartment Switch on the instrument and start the UVS software The cell changer will be recognised by the software during the initialisation process Access to the cell changer is possible in all modes using either direct control in the acquisition screen for single measurements or integration into a method for automatic processing of samples The cell changer mode can be set as described below during either of these processes 3 1 2 Cell Changer Mode only UVSX The cell changer allows three different operation modes and the mode is selectable via the Cell Changer dialogue in for example the Acquisition screen Instrument Control Cell Changer Photometer Control Mode 6s 1r v Autozero Wavelength nm Position 1 4 Set 580 0 Set Method ff Acquisition Status Values VWavel h Ab Method i Sample avelength nm l S Samples 1 Sones of Blank Value 0 0000 Samples Click on the drop down menu in Mode to select the required mode from a list UVS Software for Manual Version 2 20 Oct 2007 Page 184 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL US
169. owing calculations will be performed 1 Wavelength number 1 e g at 400 nm is added to Wavelength 2 2 Wavelength number 3 e g at 600 nm is added to Wavelength 4 3 Wavelength number 4 e g at 700 nm is divided by Wavelength 1 4 Wavelength number 2 e g at 500 nm is multiplied by Wavelength 3 Decimals The number of decimal places to be used for the calculation can also be defined This number should generally be consistent with the actual measured result e g by measurements to 4 decimal places selection of a much higher number for the calculation result will not increase the accuracy of a measurement or result and could produce rounding errors UVS Software for Manual Version 2 20 Oct 2007 Page 96 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 4 5 2 4 Calculation alculation Abs I oT abs Yad DuSoTec GmbH Dumas Software Teclewk GmbH The calculation selection permits definition of the mode of calculation to be used the user can select between either absorbance or transmission 2 4 5 2 5 Audit Trail in Wavelength Program Method Er Click on the Audit Trail icon to view the audit trail window for a method 2 4 5 3 The Report Screen in Wavelength Program Click on the Report button in the upper left corner to open the Report window Piwavelenath program n a Se mimon MB emou E Repon cizi A BG m gt F prerie protec A 337011 155317 Sl B 33 7030 99
170. p Filter change 1 5 Bandwidth Cell changer UVS Software for UVIKON 900 XL XS This parameter defines the lamp change wavelength The deuterium lamp is used for all wavelengths below the lamp change wavelength The tungsten halogen lamp is used for all wavelengths above the lamp change wavelength When checked the tungsten lamp will be switched on and used according to the setting for the lamp change When checked the deuterium lamp will be switched on and used according to the setting for the lamp change This parameter defines the wavelength used to switch the filter Selects the spectral bandwidth of the spectrophotometer Defines the operating mode of the cell changer if fitted Manual Version 2 20 Oct 2007 Page 46 of 202 UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi UY 24 1 33 Calculation alculation T Math Function me Exclude rows Statistic za Walt Control Target value O00 Calculation Deactivate Calculation for normal acquisitions without any special calculation Activate Calculation for special calculations defined under Math Function Math Function This function depends on the grid of absorbance values Enter the calculation desired Examples A1 A2 A4 A3 F A1 A2 A1 1 A2 1 F Table Matrix Example l The first number indicates the different measurements of one sample the second number indicates the sample number E g A2 1 is the first replicate of th
171. phic images exporting possibility Open after export resulting file will be opened right after exporting via HTML files viewing program which is allocated in OS by default Export peculiarities export may consist of several files Each graphic image is supported and saved in their own separate file RichText objects are transferred as simple text Appearance and file volume depend greatly on report design Report Design References chapter 2 8 31 Export to Text Format Usual text file It contains information from report This information is optimized to the limit and converted in accordance with the given format peculiarity Export method is a table diagram one On exporting to text format the dialogue box for output file parameter settings appears UVS Software for Manual Version 2 20 Oct 2007 Page 169 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DeoTes GmbH UY Export to Text Page range All Current page O Pages Enter page numbers and or page ranges separated by commas For example 1 3 5 12 Export properties Page breaks Erpty lines _ Frames _ OEM codepage Open after export Cancel Export parameters Page breaks export of page breaks to resulting file Empty lines export of empty lines Frames export of text objects frames OEM codepage resulting file OEM coding selecting Open after export r
172. ple ppa rom a Dispense mm L Bid Fill with x order x Step mm Y Step mm No of Pos in No of Pos in Y Rack width rm No of racks a z C amp R Created from admin Date 31 10 2005 12 49 56 n TERRE admin Date 31 10 2005 12 50 57 x Cancel Fill the parameter as decribed below 1 X Position This is the first vial position in the upper left corner of the rack regarding the the zero point position of the needle in x direction 1 Y Position This is the first vial position in the upper left corner of the rack regarding the the zero point position of the needle in y direction X Step Distance between 2 adjacent vial position in x direction Y Step Distance between 2 adjacent vial position in y direction No of Pos in X Number of positions per rack in x direction No of Pos in Y Number of positions per rack in y direction Rack width Width of a rack in mm No of racks Total count of racks on the liquid handler If you have a rinsing tube on the liquid handler please fill the position of this tube in the rinsing station section UVS Software for Manual Version 2 20 Oct 2007 Page 195 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DesoTeo GmbH UY How to prepare the sample positions The sample position in the racks for acquisition must have the exact the same order as defined here Please check the positions before you start the acuisition o
173. port template which already contains three bands Report title Master data and Page footer Let us remove the Master data band for a while to do that click either on any free space inside the band or on its header and then remove it by pressing the delete key or using the corresponding section in the context menu Now let us add a new band Page header To perform this click the Add band button and select Page header from the drop down list We see that a new band is added to the page At the same time the existing bands were moved down Report Designer designer automatically positions bands on the page and as a result header bands are positioned on the top data bands are in the middle and footer bands are at the bottom Now let s add some objects Add a System text object in the Page header band and select System variable in its editor DATE you should remember that the date can be displayed in a Text object by typing DATE in its editor We add a Text object which will contain the Hello text in the Report title band Note you can see the text object which displays page number is already added to the Pagefooter band UVS Software for Manual Version 2 20 Oct 2007 Page 148 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL Bed live ReportTitle ReportTitle1 r 1 Hella L l PageHeader Page
174. r change 2 370 nm hive J ne i i Test2 o ileal Decals TA Fite change a 460 nm ARJAT Abe STS o Renicsle fi EA Fiter change 5 ria nm revival Mulligan Disor O Caution Cell changer inser Wah Fure Excide rows Stal ketic a i pall ata Target value 0 00 Commentradion ft Retten rene Rew ka bratond EN Created trom eemi Date aa 1H 2008 0983 20 Remark Last modification p re acimin Date os 02 2004 11 2227 ss m i 52 02 Frihed Select the Acquisition screen insert the samples in the cell changer and set the number of samples to the appropriate value Check whether an Autozero is required before starting the measurement If so click the Autozero checkbox in the Acquisition screen Caution Before executing the Autozero ensure that the cell changer if used is actually in the correct position The Autozero will be executed at the current position which may not be position 1 Measurements with the cell changer always start from position 1 Press Start after measurement the Acquisition and Parameter screens obtained are shown below The main screen contains the measured absorbance values the result and the calculated concentration values UVS Software for Manual Version 2 20 Oct 2007 Page 115 of 202 UVIKON 900 XL XS USER MANUAL pes at JE i nemoe oo aE The Parameter screen contains the value used for the Factor used to obtain the result the formula used to calculate the act
175. rammed by removing the write protect check box tick and clicking on the icon The available functions for each method type are described in the appropriate sections below UVS Software for Manual Version 2 20 Oct 2007 Page 37 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DeoTes Ombi U 2 3 10 Saving methods The system prompts for a name for the method to be stored whenever a new method is to be created Generally methods cannot be simply overwritten because of the tracking system used for CFR Part 11 therefore it is necessary to enter a new name and confirm with OK Please enter name to sawe Once the parameters for the method have been entered the method is saved by clicking on the green tick in the option tool bar write protect 2 3 11 Cancel all current activities Clicking on the Exit icon in the top left hand corner of each screen will return automatically to the start up screen Clicking on the Stop button in the acquisition screen will cancel any currently running data acquisition etc Caution lf you exit without having saved your data on disk you cannot restore the data A message will warn you if this situation occurs 2 3 12 Activate Cursor and Manual Peak Picking a The Activate Cursor icon can be used in Wavelength Scan Time Drive and Auto Rate Assay methods to quickly overview values of the sample curve on the screen In the Report Overv
176. rch filters is available Date Text etc e The copying of data from one archive into another archive or the current data base is not possible e Users and Powerusers can also call up and re process archived data if the appropriate permissions in User Management have been set Backup Execution of Backup in the Backup Is saved with the Backup if a directory for the Archive was selected in the Database directory All files are saved in the Database directory including sub directories Two files are created bck File extension for zip File extension the re importing using for the manual re the Restore tool import procedure supplied using an Unzip program e g Winzip UVS Software for Manual Version 2 20 Oct 2007 Page 22 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL WS OT USER MANUAL es 2 2 4 User management The UV Vision has its own login facility A login name and a password is required to enter the program The assignment of user rights password administration etc is made by the administrator in a separate dialogue box accessed by clicking on the Login key in the main display p Security User Groups m alem ead eel ed e write protect Login name domain group LocaliDomain gt Full Name adinin Domain ADomain Group Security Login rights Saving Data Password required Overwrite alowed Login blocked Fi Crea
177. rd 26 2 2 4 2 Groups 26 LS E ae ee ee eee ne ore ee eee eee eee or eee eee eee nee Ce eer ae 27 ELU FONT O E E OET 28 2 2 7 Self test and system software initialisation ec ececesececeseeeeceneeeseaneeeseueeessaneneneaes 28 2 2 8 Exiting the system software ccecccccceeeceeeceeeceeeneeeceeeseeeseeesaeeseueeeeessueeeueeeeeseeeneeenes 29 E PER T renew rics ses ee sietg ene aie mec i iviee es 29 ee IT ee ee i tance een eer viene ene eee neers 29 lt UVS Software for gt Manual Version2 20 Oct 2007 2 _ _ Page 2 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL a UVY 2 3 1 1 Acquisition Calculate Display 29 2 3 1 2 Recommended Operating Practise 29 2 3 1 3 The Acquisition Window 30 2 3 1 4 The Method Window 32 2 3 1 5 The Report Window 32 2 3 1 6 Quick Search Function and Row Colouring ao 2 9 2 Starting a meas uremenMlseissirsuiisniisninrrednnenda ndien nnna ENARE KENNER EITA E E NA ERE a 34 Ba P A E ea eden eer emaueeetner tees 34 e Fe rir eee ed nen E AE E dae ee ie ele ieee 35 2 3 5 Loading existing data fIlOS ce ecceccceecceeeceeeeeeeseeeseeeseeeseeeseeeseeeseeeseeeseeenseeneeeneeenes 35 23 6 Data conversion to EXCEL formal ate ei rateceedecdeciciesderieoenteeiveierseoreeeeieeerieneeraenees 36 Es E E AE 36 E P E E ee ee ee ee en T eee ere cee eee eae eee eee 36 Br sci i i es tess 36 ies Eee E Eee tence Tene eee t one Te nee MTree ten Teeter a renenr rare ren reenter
178. remit retreat tert 38 200 1 Ve Canca all cune aoii OE naii n nn aE EE 38 2 3 12 Activate Cursor and Manual Peak Picking ccccceeceeeeeeeseeeeseeeeseeeeeseeeeeaeeesaeees 38 IE t AREER AE EE AT E EESE TET EEATT T ATEEN T 39 Laeta radna Commento O CONVOI serisine aE 40 e a E E e EA EE EE A EEEE AEE ALOLE E T IET T oes 41 Ee og 218 Bg a T ee nn E A E A A EAE 41 2 4 1 1 The Acquisition Screen in Fixed Wavelength 41 2 4 1 1 1 Statistic 42 2 4 1 1 2 Instrument Control 42 2 4 1 1 3 Run parameters 42 2 4 1 1 4 System Status 44 2 4 1 2 Parameters in Fixed Wavelength Acquisition 44 2 4 1 3 The Method in Fixed Wavelength 45 2 4 1 3 1 Acquisition parameters 45 2 4 1 3 2 Common Parameters 46 2 4 1 3 3 Calculation 47 2 4 1 3 4 Concentration 48 2 4 1 3 5 Audit Trail in Fixed Wavelength Method 48 2 4 1 4 The Report Screen in Fixed Wavelength 48 2 4 1 4 1 Printing 49 2 4 1 4 2 Importing a report from the database 49 2 4 1 4 3 Filtering the report list 49 2 4 1 4 4 Audit Trail in Fixed Wavelength Report 50 2 4 1 5 Calculations in Fixed Wavelength 50 2 4 1 6 Concentration in Fixed Wavelength 51 pe eh EEEE EEEE 51 2 4 2 1 The Acquisition Screen in Wavelength Scan 51 2 4 2 1 1 Run parameters 52 2 4 2 1 2 System Status 53 2 4 2 2 The Method in Wavelength Scan 53 UVS Softwarefor gt Manual Version 2 20 Oct 20077 Page 3 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL 2 4 2 2 1
179. rent page C Pages Enter page numbers and or page ranges separated by commas For example 1 3 5 12 Export settings Styles Merge cells Pictures WYSIWYG As text Background Fast export Page breaks Cancel Open Excel after export Export parameters Styles transferring of text objects design styles into the table Disabling increases exporting speed but worsens document appearance Pictures includes graphic images export into output table As text all objects are transferred into table diagram as text ones This option may be useful when transferring numeric fields with complicated formatting Fast export usage of optimized fast data transferring to Excel This option disabling slows down data transferring but increases export compatibility on any errors during data transferring Merge cells cells integration in resulting table diagram for achieving maximum correspondence to the original Disabling increases exporting but reduces document appearance WYSIWYG full compliance to report appearance On this option disabling the optimization for reducing the number of lines and columns in resulting table is performed Background export of filling color assigned to report page Page breaks includes page breaks in Excel Open Excel after export resulting file will be opened right after exporting into Excel Export peculiarities Excel program must be installed on y
180. s opened automatically and the data entered in a worksheet 2 3 7 Export to Clipboard Data files in graph format can be exported to the Windows Clipboard to be immediately imported into other running programs via the copy paste mechanism ig Click on the clipboard icon in the graphics toolbar and the curve is exported automatically to the clipboard for further processing 2 3 8 ASCII Export For interfacing with a LIMS system ASCII export is possible from Acquisition and from Report mode If the sample set has been stored as a report then the ASCII file gets the same name as the report itself with the extension txt These ASCII files can be directly read by the LIMS systems 2 3 9 Methods The system will always use a set of parameters called a Method to execute the data acquisition and measurement step The method file for an acquisition is selected from a drop down list in the main acquisition window as described below each measuring mode Simply click on the required method in the list Programming and viewing of methods is made from the Method screen for each measuring mode Existing methods are shown in a list in the left hand window clicking or scrolling through the list allows an easy overview of the current method in this list UVS Software for Manual Version 2 20 Oct 2007 Page 36 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes GmbH UY A new method is prog
181. st in the left window will become very long with time and it may be necessary to filter the list so that only files of interest are shown to a user This is carried out using the Select Report icon rs Click on the Select Report icon and a dialogue window appears which allows selection of a H view by date method user or name Select Acquisitions hf Range from 13 09 2000 to 13 09 2000 Iw Method ozi 218_ calibration Method Revision Name User The appropriate checkboxes must be checked and where applicable either a date or name selected Clicking on OK will cause only those reports which satisfy all selected criteria to be shown in the list of available reports UVS Software for Manual Version 2 20 Oct 2007 Page 75 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _ Dado Tes Gabit U ad 2 4 3 3 5 The Chart Report Screen Clicking on the Chart tab will open a new screen containing the curve in a form which can be edited 18 a Acquistion WE method Report aa A BO E Oxo D verie protect len 820 x E gaa bumen ofesoaanoe tones To edit in cursor mode uncheck the write protect checkbox first Navigation arrows are available to move between the various reports contained in the list without the need to return to the Overview screen The name of the currently displayed curve set including the revision is shown in the box display i
182. st of all methods used for the given measuring mode Scrolling through the list updates automatically the corresponding fields in the parameter definition fields Parameter Definition All parameters necessary for the method are defined and displayed here Current Method History Displays data relevant to the creation and history of each method in the list Cos la The Report Window The Report page layout is similar for each mode and is shown below Graphical display functions are again only available in those measuring modes where graphics are actually used UVS Software for Manual Version 2 20 Oct 2007 Page 32 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL DuSoTec GmbH Due Software Tomik GmbH IUY W scan E 8 x Z Acquistion HH Method EA Report Working Area Selection Bar ris Jf gt mI IV write protect Selection Toolbar Name Rev Date User hoo 0 16 02 2003 18 11 37 admin Nitrate Test 1 0 22 02 2003 17 34 50 admin 1 G No 1 gt 4 22 02 2003 17 42 13 7 Curve Display Area Run Details Available Reports List Method Rev nitrate Test 1 0 Scanintervall 1 0 nm 1000 nmmmin 350 400 450 500550 600 650 700 750 Remark Signature Comments Area Signature J DetailsSigned Rev Overview 17 42 46 User admin Working Area Selection Buttons for selection of working window e g Method Selection Toolbar
183. t 580 0 Set Method y Acquisition Status Values 7 h l Method 7 Sample Wavelength nm bs Samples 1 Sole Blank Value a Samples 3 1 3 Cell Changer operation in the different system methods es a IB 2 Auto rate assay In this method the instrument can measure up to ten samples simultaneously when the cell changer is in use Measurement starts with the first reading on sample 1 The cell changer then moves to the next position and measures the first reading on sample 2 This process continues until all selected samples parameter No OF SAMPLES have been measured once The instrument will then continue measurement with the second reading on sample 1 and all other samples The whole process is repeated until the selected number of readings has been measured Up to ten curves depending on the selected number of samples can be displayed on the screen during measurement lt A a Fixed Wavelength Measurement starts with the first sample After the selected Averaging Time the cell changer will move to the second sample Only one reading per sample is measured and displayed The cell changer can also be used to measure standards in Calibration mode Shaa Wavelength Scan The complete scan is carried out on the first sample before the cell changer moves to the second position and measurement of sample 2 is carried out This process continues until the selected No of samples has been measured 3 1 3 4 Time Drive The co
184. t F Factor and VM represents the measured values obtained for sample and blank respectively UVS Software for Manual Version 2 20 Oct 2007 Page 50 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DedoTes Gabit UY Recalculation after for example changing the factor value is possible at any time by clicking on the Calculator icon 2 4 1 6 Concentration in Fixed Wavelength In simple cases the Result obtained above can be used as a concentration value if the Factor has been defined and zero concentration corresponds to zero absorbance Using the calibration function it is possible to calculate concentration values based on a previously determined calibration curve This method offers the advantage that any absorbance offset is compensated for and that the final result is given in the desired units in the report print outs The calibration uses the value obtained for the Result see above and converts this value to a corresponding concentration value in units which are defined as part of the calibration file A more detailed description of the Calibration function is given in a separate section within this document 2 4 2 Wavelength Scan 2 4 2 1 The Acquisition Screen in Wavelength Scan Select Wavelength Scan from the main menu to obtain the following display a rrr eee IEF e a 4 e Acqustion MB Method E Repon Sed ee a SES jase eG 0 apla bt TOht Chet P
185. t is the minimum step between axis labels It must be either a positive number or DateTime value This value will be used as the initial step value If there is not enough space for all labels then a larger increment will be automatically calculated This value defines only the increment used on the scale it is also possible to format the number of decimal places required as described below Logarithmic Check this box to obtain a chart based on the corresponding logarithm 2 9 2 1 2 Decimal Format The decimal format required can also be set from within the Axis tab Click on Labels and then on the Format tab to obtain the following window 2 x Axis Titles Legend Panel walls 3D Iv Visible Scales Title Labels Ticks Minor Position Jf Behind Anes Style Format Text Exponential Values Format 0 0000 W Default Alignment UVS Software for Manual Version 2 20 Oct 2007 Page 176 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _ Dado Tes Gabit TL Enter the required decimal format in the Values Format box e g for 4 decimal places enter 0 0000 2 9 2 1 3 Title This allows formatting of the name to be used for a given axis e g Absorbance The tabs allow definition of the name its alignment relative to the axis and also the font and test appearance to be used 2 9 2 2 Titles Normally only the definition of a chart titles
186. t signal value over the entire scan range from 220 to 500 nm MAXL provides the wavelength for the highest signal value over the entire scan range from 220 to 500 nm 2 4 2 2 6 Audit Trail in Wavelength Scan Method a Click on the Audit Trail icon to view the audit trail window for the method 2 4 2 3 The Report Screens in Wavelength Scan 2 4 2 3 1 The Overview Report Screen Click on the Report button in the upper left corner to open the Report window UVS Software for Manual Version 2 20 Oct 2007 Page 57 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL _DusoTec GmbH IUY E ale x E i Te Acouiction BB Metr Ee Report gt d HE n M E Sanpet Method Rew Rechertest Scan inlerval 1 1 1 1 1 0 ram E x eg Import database X a Year 2002 Signature Approved 7 Accepted 3 Signed Rev Cancel Help Creer chert Table ieis Finthed Der aamin The display obtained is the Overview screen clicking on the Chart tab will display the chart as the main item which can be manipulated or edited as required The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list 2 4 2 3 2 Printing Click on the Print icon to obtain a print preview of the selec
187. te additional report Method Rights Allow user to modify Report Rights Allow user to delete Allow user to modify Power User Member of group Show reports of all users Option Rights Allow user to modify Maintenance Rights Allow user to modify projects Allow user to exeucte Backup and Archive Allow user to modify extras Allow user to backup Allow user to archive Calibration Rights Allow user to execute Signature Rights Permitted Actions Approval Accept UVS Software for Manual Version 2 20 Oct 2007 Page 23 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DeSoTes Ombi TL 2 2 4 1 Password 22444 User rights Generally three different user types are recognised with basic default permissions as described below Action CSCSCSCSC dt Administrator Power User User Fix Wavelength yes Timedrive Option Depends on Depends on Depends on security rights security rights security rights Access only to Access only to print controls print controls Main window Backup Edit Security Trail Service Password required on saving data Overwrite existing report on saving data Create additional report on saving data Change Method Delete Report Modify Report Signature approval Signature accept re a Report Only own or Only own or grouped grouped member mem
188. ted report which will always contain all measurements The report can be printed by clicking on the Print icon on the preview page 2 4 2 3 3 Importing a report from the database Click on the Import icon to access earlier reports in the archived database A dialogue window for selection of the database will be opened UVS Software for Manual Version 2 20 Oct 2007 Page 58 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DeSoTes Ombi U 2 4 2 3 4 Filtering the report list The report list in the left window will become very long with time and it may be necessary to filter the list so that only those files of interest to a user are actually shown This is carried out using the Select Report icon Click on the Select Report icon and a dialogue window appears which allows selection of a view by date method user or name Select Acquisitions l x fv Range from fzoa2000 gt to fiz032000 fw Method JO31218_caltration l Method Revision _ Name Po User The appropriate checkboxes must be checked and where applicable either a date or name entered Clicking on OK will cause only those reports which satisfy all selected criteria to be shown in the list of available reports ee SP aise feo The Chart Report Screen Clicking on the Chart tab will open a new screen containing the curve in a form which can be edited UVS Software for Manual Version 2 20 Oct 2007 Page 59
189. the method 2 4 1 4 The Report Screen in Fixed Wavelength Click on the Report button in the upper left corner to open the Report window I ziez e a Bri 42 8G FP FF rheme E T Nene I 00023 0 0020 ETE a TETE Dser admin UVS Software for Manual Version 2 20 Oct 2007 Page 48 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL _DeSoTes Ombi UY The left hand window contains a list of all available reports the right hand window contains the results in the upper section and the main parameters for the measurement in the lower part Each report can be viewed by scrolling through the left hand list 2 4 1 4 1 Printing Click on the Print icon to obtain a print preview of the selected report which will always contain all measurements The report can be printed by clicking on the Print icon on the preview page Import database x Year 2002 2 4 1 4 2 Importing a report 2 TER Help from the database Click on the mport icon to access earlier reports in the extracted database A dialogue window for selection of the database will be opened 2 4 1 4 3 Filtering the report list The report list in the left window will become very long with time and it may be necessary to filter the list so that only those files of interest to a user are actually shown This is carried out using the Select Report icon re Click on the Select Report icon and a dialogue window appears wh
190. therwise the system get the wrong sample In this example the sample positions are filled with y order 8 58 S16 524 532 540 548 Example of a sample with 2 replicates in a rack with 4 columns The 4 column is free Gilson method Sampler Rack H a gt mH p a C Write protect Rack Code a Rack Rinsing station Sample D 221 aein 10 Xx Rinsing Station Z Sample Fill with y order mm mm q Lad Aspirate mm 1 Y Position n S 3 Z Sample Te Imm E 10 eae Station Dispense mm Fill with order x Step mm a 10 Z Rinsing Station Y Step mm 10 a No of Pos in 4 No of Pos in Y 11 Rack width mm 100 No of racks e no Remark Created from admin Date 27 10 2005 12 40 13 Last modification a admin Yy OK x Cancel UVS Software for Manual Version 2 20 Oct 2007 Page 196 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _DesoTeo GmbH UY How to use it Create a method with 3 No of pos in x and fill with x order Gilson method Sampler Rack r v x C Write protect ny AUER Rack z T M Rinsing station Sample T ao fe Staton ann Z Sample i 100 Fill with y order mm Aspirate mm 1 Y Position Z Sample 40 ae Station Dispense mm x Step mm ZRinsing Station Y Step mm ira No ofPos inX No of Pas in Y Rack width mm N
191. this is not a file export and the data can then only be saved and processed within Excel Imports an earlier extracted database it Opens an edit dialogue for reporting functions Exports a curve or table to the Windows clipboard for further processing Opens the calculation dialogue window or executes a re calculation S r Opens the audit trail dialogue to allow viewing of the audit trail for a document 5 Opens the signature dialogue to allow signing of a document IN UVS Software for Manual Version 2 20 Oct 2007 Page 10 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL IU Y pry Shows position of values on X and Y axis and allows to manually pick peaks and to add edit or delete comments UVS Software for Manual Version 2 20 Oct 2007 Page 11 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N AT USER MANUAL uate Duss Software Tomik Gm TUS 2 2 SET UP 2 2 1 Starting Ensure that the Uvikon is connected to the computer and is switched on Then click on the UVS icon to start the software The unit will first initialise a prompt will appear for user name and password User name and password are necessary because of the requirements of CFR Part 11 and permit only certain actions for users depending on the assigned permissions F UV VISion ME Exit Maintenance Register Trail Help A oa iE Fixed Wavelength fil Wavelength Program SH wavelength Scan
192. tionary measure to ensure that the system access password is known only to the user and no other third party 2 2 4 2 Groups This defines the grouping for report access Groups and members of groups can be defined to view the reports A member of a group can view the reports of all members User in group Modit Workstation group UVS Software for Manual Version 2 20 Oct 2007 Page 26 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL _DeSoTes Ombi TL 2 2 5 Baseline The UVS software does not automatically execute a baseline at start up This operation can be executed by clicking on the Baseline Run icon in the start up screen to open the following dialogue window ethod Lamp change 330 Lower 300 Wavelength mn Tungsten Lamp Iw Deuterium Lam y Upper 400 nm po M Wavelength Fiter change 1 260 Fiter change 2 ari Fiter change 3 460 Fiter change 4 sro Speed f High speed Low speed Fitter change 5 710 OK Cancel The actual conditions under which a baseline is executed can impact later measurement results if these are made using different parameters such as scan speed For this reason the UVS software requires a method to be defined in Wavelength Scan mode before a baseline can be executed Click on the selection button adjacent to the method box in order to select the correct method if this is not already displayed This method is then displayed
193. ton adjacent to the revision box UVS Software for Manual Version 2 20 Oct 2007 Page 52 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL USER MANUAL _ DeboTeo Gabit LY E xj Method Revision Last Change 30 07 2004 1218 54 Test C3007 204 1078 neu 0 300 20044 102447 This window contains a list of all available methods including all revisions of the method and the date of last modification Selection is made by highlighting the required line and then clicking on OK The order of Methods may be displayed by usage or alphabetically Wavelength A certain wavelength may be chosen manually and set immediately in order to allow rapid monitoring of the unit 2 4 2 1 2 System Status The lower right section of the display contains the actual status of the system and displays the following parameters gt Current sample being measured gt Actual wavelength gt Actual absorbance value gt Temperature where applicable 2 4 2 2 The Method in Wavelength Scan Click on the Method button in the upper left corner to open the Method window UVS Software for Manual Version 2 20 Oct 2007 Page 53 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL Nh A USER MANUAL oo Duss Software Tomik GmdhH U laix a Method A Report I a gt gt i V write protect S4 E Ez V Show only active methods Method Revision Used dummy 0 Method not active i Lamp chan
194. tware Tomik GmbH Opens the calculation dialogue window Exports a curve to the Windows clipboard for further processing Prints the curve as a single sheet report Formatting of the report is described in a separate section of this document dealing with curve format Opens the edit dialogue for formatting functions See the separate section of this document dealing with curve format for more details Converts chart format to a 3D display The icon functions as a toggle switch between a 2D and 3D display Click on this icon to control the depth of the 3D display using the mouse cursor Clicking on this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Double function icon allowing zooming of the complete curve Click on this icon to move the complete curve display using the mouse cursor Clicking on this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Click on this icon to rotate the complete curve display using the mouse cursor Clicking on mn this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Clicking on this icon will return the function of the mouse cursor to normal thus allowing use of the normal zooming functions etc in 3D displays 2 4 2 3 7 Audit Trail in Wavelength Scan Report ay Click on the Audit Trail icon to view the audit trail window for the report
195. ual concentration and also the units e g ug ml mg ml etc may be entered 3 saree ET 0 000 235 E UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DesoTeo GmbH U 2 5 6 Quadratic Calibration Calculation of concentration by quadratic regression using standards The practical use of this calculation mode is almost identical to linear interpolation see above One additional parameter is used with the following formula Y Ax Bx C The process of obtaining the calibration data is identical for both the Linear and Quadratic calibration methods until selection of the calibration order is made Select the Quad function in the Calibration parameter dialogue Calibration parameter x Orde Linear Quad Cancel Help The calibration curve will now look like this W Calibration Fixwavelength a la x a 2 SS Acquisition E Method A Report y 0 784385x 0 205950x0 000000 2 1 000000 0 1 14 47 48 Finished User admin UVS Software for Manual Version 2 20 Oct 2007 Page 117 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N USER MANUAL _DeSoTes Ombi U 2 6 REPORTING This section deals only with the possibilities for formatting and printing reports in the UVS software The main focus is on the treatment of curves since all table format printouts are pre defined 2 6 1 Printing The UVS software
196. umeric characters and press OK The comment will appear directly linked to the curve at the cursor position on the X axis More comments may be added by repeating the same procedure The comments are numbered and the spin selector by the Comment No box can be used when editing or deleting comments in order to select the item to be used UVS Software for Manual Version 2 20 Oct 2007 Page 40 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL WS AT USER MANUAL Trivia 2 4 SYSTEM MODES This section explains the parameters and gives a step by step measurement example for each system mode Many parameters are common to all methods but are explained separately for each mode in the interests of clarity 2 4 1 Fixed Wavelength 2 4 1 1 The Acquisition Screen in Fixed Wavelength Select Fixed Wavelength from the main menu to obtain the following display TTT ss aleix a o RS Acetic E Meter E eni ES ET M uaa a uD Oa E 52 TEIE 050 apo oaa Dan cA D Aii ima D a 604 i Samples Hh Replicates Depends on results not shown if calculation is deactivated ft Entice EE Liaw 4 4 0 a0 0 80 Method 595210 _cokbe ston p_e e WVavekength rn 12 18 06 Pried Leer admin This is the acquisition window from which all measurements are started In this window the following parameters must be selected before starting a measurement Method to be used for the acquisition The method
197. ur PC RichText objects are transferred as a simple text graphic images are not supported 2 8 29 Export to CSV Format CSV file contains values formatted in the form of a table diagram and adjusted in such a way that every value in column is divided from value in the next column be means of separator and every new row begins with new line This format may be imported into different table diagram editors Export method is a table diagram one On exporting to CSV format the dialogue box for output file parameter settings appears UVS Software for Manual Version 2 20 Oct 2007 Page 167 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL YS AT USER MANUAL _DesoTes Guba UY Export to CSV Page range All Current page Pages Enter page numbers and or page ranges separated by commas For example 1 3 5 12 Export properties OEM codepage Separator i Open after export Export parameters OEM codepage resulting file OEM coding selecting Separator values separator in files Open after export resulting file will be opened right after exporting via CSV files viewing program which must be installed in OS Export peculiarities on transferring into this format report design is not saved Graphic images are not supported 2 8 30 Export into HTML Format HTML Hypertext Markup Language is regarded as standard language for document marking in the Internet HTML was
198. using the button In order to do this it is necessary to uncheck the write protect checkbox The system will first prompt for name under which the new method will be saved Once this has been defined all other parameters can be entered UVS Software for Manual Version 2 20 Oct 2007 Page 71 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL DuSoTec GmbH Dussp Software Tomik GmbH 2 4 3 2 1 Acquisition parameters Method not active Wavelength Delay time Averaging time Measure time Sampling rate Autozero at start Auto Calculation Calculation mode Inactivate and thus hide the current Method by checking this box Defines the wavelength to be used for the measurement The instrument will wait for the selected delay time after the MEASURE key is pressed to start the actual measurement Defines the time the instrument uses to collect data for calculation of a mean value for one reading A long averaging time is useful to reduce noise when measuring at high absorbance values Defines the actual duration of the measurement in minutes Defines the number of data points per minute for the measurement Defines whether an autozero is to be executed beforeeach sample run If checked the calculation for peak detection smoothing etc starts automatically after acquisition using the defined parameters Calculation and curve portrayal can be in either Absorbance or Transmission units
199. using the cell changer Select the method to be used and set the number of samples to 6 Insert the samples into the cell changer and start the measurement by pressing the Start button The measured curves are displayed in real time during acquisition A 3D display see below may be used to give better clarity if the curves are very similar for each sample The final window will look similarly like this oa lB a e S acquistion MB Method EA Repon Hfst met use 0 700 4 o eso0 4 05000 4 05500 o5 4 4 5a T4000 2 03500 4 a 3000 ered 0 2500 oan OSU fF oom J 0050 p Phgtomenter Canny Avozero Wavelength nm iethod T Values Meihed Recre Pu Saroe 400 0 0 1204 echenedi i 40 0 0 0 1 204 Zapier frii zE E 08 Fin had l User sann Note If you are not using a cell changer you will be prompted for the next sample after each sample has been measured The final appearance of the screen remains unchanged Individual curves may be removed from the overlay display by clicking the checkboxes in the legend on the right of the curve If you repeat the measurement you will always measure the selected number of samples 6 in our example until you change the Samples parameter The system will prompt if the previously run sample data has not been saved beforestarting a new run A Data not saved Continue anyway UVS Software for Manual Version 2 20 Oct 2007 Pa
200. window which opened by clicking on the button adjacent to the revision box Press this button to accept the number of samples the sample grid will also created Surveys the temperature of the sample Methods eee ZI Method Alevition Lint Change OST TS cabi 031217 _fesach_1 1 F412 2003 10 58 25 11007218 _cattrason 2 2212203105743 0217 fect 017 12 5008 132656 ang catbrason 119122003 131258 01718 cibin Sii 0 1812203 103 091218_cakbraton 2 19122003 1224 Ov 223 bess 1120 20 13 06 25 OHA cabesso reom 019122003 11 11 53 Tis esile 0 2312203 11 10 20 Oa 1217_feoeched D 17 12 2003 141406 11091218 kal supe D 18 12 3003 10 65 29 Cider r TE ie be Lr depi i dohabelicaly This window contains a list of all available methods including all revisions of the method and the date of last modification The order of the shown methods may be selected by ticking by usage or alphabetically in the lower right corner of the screen Selection is made by highlighting the required line and then clicking on OK The operating mode of the cell changer and the actual position can be defined here before starting a measurement series During the Dialogbox Please insert replicate it is possible to move the cell changer with the and key Manual Version 2 20 Oct 2007 Page 43 of 202 UVS SOFTWARE for UVIKON 900 XS XL WS OT USER MANUAL Te 2 4 1 1 4 System Status Wavelength nm A
201. x and then save the calibration under the name required pan WE pe auii agii 0 001 QA UVS SOFTWARE for UVIKON 900 XS XL N A USER MANUAL Agate Duss Software Tecleuk GmbH 1 IU Y Values may be invalidated by double clicking them Invalidated values and their respective measure points in the Chart are marked red R calibration Mawavelength y PU altel ol EI Rte 02568 The data for a calibration can be viewed at any time by selecting the required report in the Report screen Click on the Chart icon and the calibration curve will be displayed together with the regression values y 0 946622x 0 003412 R 0 999946 mg ml UVS Software for Manual Version 2 20 Oct 2007 Page 114 of 202 UVIKON 900 XL XS UVS SOFTWARE for UVIKON 900 XS XL WS AT USER MANUAL IUS 2 5 5 2 Using a Calibration Curve The calibration curve can now be used to measure a series of unknown samples Exit the Calibration mode and select Fixed Wavelength and then select the Method screen Here you should click the Active checkbox in the Concentration and then select the required Calibration as Reference data Save this method and the screen should look like this i ale xl a EES e Te Acquistion E meroa Gah Report Method not ective a Lanp change 333 nm Test e a A Tungsten Lamp fur Teall j Pireler 400 0 nm DeuterumLanp F fest ar Delay op a Fiter change 1 280 nm gee A ip Fite

UVSX Manual WD Version 2.20 21-10-07

Contents

Download Pdf Manuals

Related Search

Related Contents