User`s Manual - Web Hosting in Massachusetts MA and New
Contents
1. IS Company StatSpin Inc Tel 800 782 8774 85 Morse Street 781 551 0100 Norwood MA 02062 Fax 781 551 0036 StatSampler Patent Number 5 257 984 StatSampler and StatSpin are registered trademarks of StatSpin Inc an IRIS Company SS2E 1EF p65 9 08 00 54 002861 001 REV F2. S H Simultaneously obtained skin puncture serum skin puncture plasma and venous serum compared and effects of warming the skin 5 Fill the Capillary tube to the end fill volume is approximately before puncture Clin Chem 23 1705 1977 3 Meites Samuel Skin Puncture and Blood Collecting Technique for Infants Update and Problems 200 uL Clin Chem 34 1890 1894 1988 iy A 7 i 4 Burns Edward R Development and Evaluation of a New Instrument for Safe Heel stick 6 Turn the assembly to the vertical position the blood will drain Sampling of Neonates Lab Med 20 481 483 July 1989 into the Micro Tube See Figure 2 To discharge the last drop of blood into the MicroTube wipe the Capillary end with a lab tis 5 sue then place a pipette bulb included in your kit over the top Stat Spin of the Capillary tube Squeeze a puff of air into the Capillary An tals Company Telephone 781 551 0100 85 Morse Street Toll free 800 782 8774 tube to expel the last drop Save the pipette bulb Norwood Massachusetts Telefax 781 551 0036 02062 USA StatSampler Patent Number 5 257 984 7 Remove and discard the Capillary tube Gently shake the Micro Tube to ensure mixing of the anticoagulant StatSpin and StatSampler are registered trademarks of StatSpin Inc ssxuh M p65 7 20 98 54 001696 001 REV M S5StatSampler gt INSTRUCTIONS FOR USE EDTA TREATED MICRO BLOOD COLLECTORS Product Numbers SS1E amp SS2E INTENDED USE For capil
3. S5StatSamplery INSTRUCTIONS FOR USE MICRO BLOOD COLLECTORS Product Numbers SS2H SS2U amp SS2X INTENDED USE For collection and processing of capillary blood to prepare plasma serum SUMMARY The StatSampler requires assembly before use by inserting the Capillary tube the end with the colored band first through the split stopper See figure 1 Finger stick blood is collected into a Capillary tube and then turned to the vertical position allowing the blood to drain into the MicroTube from which it can be rapidly separated for the preparation of plasma serum StatSampler blood collectors combine the advantages of 1 Immediate anticoagulation in the Capillary tube 2 Ease of collection in a Capillary tube 3 Reduction in technique dependency in collecting capillary blood 4 Isolation of cells by a barrier gel CONTENTS 1 50 Capillary tubes 3 3 Pipette bulbs 2 50 MicroTubes 4 10 non split stoppers ANTICOAGULANT not included in serum preparation kit Each Capillary tube contains enough lithium heparin or EDTA to anticoagulate 200uI of blood There is no anticoagulant con tained in the MicroTube portion of the StatSampler If a volume of blood greater than 200ul is required then a second Capillary tube must be used to ensure the proper anticoagulant blood ratio STORAGE Store kit in a cool dry place USE BEFORE DATE StatSampler capillary tubes containing anticoagulant should not be used beyond the dat
4. e stated on the vial label For in vitro diagnostic use PRODUCT DIFFERENCES SS2H Lithium heparin treated SS2U Untreated SS2X EDTA treated PROCEDURE 1 Label the StatSampler MicroTube with a marking pen if desired with patient i d date time 2 Assemble the StatSampler by carefully inserting a Capillary tube the end with the colored band first into the Micro Tube through the split stopper Hold the Capillary tube near the end being inserted and carefully push it in until the color ed band is even with the top of the stopper See Figure 1 CAUTION ALWAYS ASSEMBLE THE STATSAMPLER BEFORE BLOOD COLLECTION TO PREVENT UNNECESSARY EXPOSURE TO BLOODBORNE PATHOGENS AND TO MINIMIZE BLOOD SPILLAGE AND POSSIBLE TUBE BREAKAGE PROCEDURE cont 3 Perform a finger stick using good laboratory practice HELPFUL HINTS FOR COLLECTION OF CAPILLARY BLOOD SPECIMENS as outlined in the NCCLS publication Procedures for the Collection of Diagnostic Blood Specimens by Skin Puncture Second Edition Approved Standard NCCLS publication H4 A2 Villanova PA July 1986 CHOOSING A SITE 3a The site of choice in adults and older children would be the palmar finger print surface of the finger Do not use the side or tip of the finger Tissue thickness found in these locations is about half that found in the center of the finger 3b Infants under the age of one year should have skin puncture procedures performed on either t
5. el bone and surrounding tis sue Incisions between 0 35 and 1 6 mm in depth leave virtually no hematoma and allow for subsequent sampling from the same region 4 SPECIMEN COLLECTION 3f After blood flow has begun wipe off the first drop Angle the puncture site downward so as to facilitate blood flow Be certain not to milk the appendage a steady firm pressure is prefer able if required Turn the assembly to the vertical position the blood will drain into the MicroTube See Figure 2 To discharge the last drop of blood into the MicroTube wipe the Capillary end with a lab tis sue then place a pipette bulb included in your kit over the top of the Capillary tube Squeeze a puff of air into the Capillary tube to expel the last drop Save the pipette bulb Remove and discard the Capillary tube and split stopper Se cure purple cap to close MicroTube Gently shake the MicroTube to ensure mixing of the anticoagulant Note Transfer the blood in the Capillary tube into the microtube as soon as possible and mix thoroughly After mixing the sample is stable for 2 4 hours 1 Following collection EDTA treated blood should be thoroughly remixed just before use Remove and discard capillary tube and split cap Figure 2 Figure 3 LIMITATIONS StatSampler has been designed for capillary blood collection Val ues obtained from skin puncture specimens may differ from those obtained by venipuncture 1 2 Mi
6. he lateral or medial plantar surface of the heel or the plantar surface of a big toe This is to avoid injury to the infant from skin puncture of an insufficient tissue depth area Itis imperative that the depth of puncture in the infant not exceed 2 4 mm to avoid penetrating bone and the risk of osteomyelitis 3 The opti mum puncture depth of the heel in the newborn is between 0 35 and 1 6 mm beneath the skin surface as this is where the major blood vessels are located 3 SITE PREPARATION 3c Warm the site Warming of the site drastically increases blood flow to the area resulting in a larger quantity of blood collected When following proper collection procedures up to 0 5 ml of blood can be obtained from a single puncture site Warming is achieved by either applying a warm compress to the site or placing the site under warm running water The site should be warmed at a temperature not to exceed 42 C for a minimum of three minutes 3d Clean the puncture site Use a commercially prepared alcohol pad or a 75 aqueous solution of isopropanol After cleaning thor oughly dry the site with a sterile gauze pad Residual alcohol can cause hemolysis Do not cleanse area with betadine as it may cause an elevation of potassium phosphorus and uric acid 3e Puncture the site A commercial lancet device either automated spring loaded or manual is available in a wide variety of styles for uniform depth punctures The Tenderfoot by I T C shou
7. l the StatSampler MicroTube with a marking pen if desired with patient i d date time 2 Assemble the StatSampler by carefully inserting a Capillary tube the end with the colored band first into the MicroTube through the split stopper Hold the Capillary tube near the end being in serted and carefully push it in until the colored band is even with the top of the stopper See Figure 1 CAUTION ALWAYS ASSEMBLE THE STATSAMPLER BEFORE BLOOD COLLECTION TO PREVENT UNNECESSARY EXPOSURE TO BLOODBORNE PATHOGENS AND TO MINIMIZE BLOOD SPILLAGE AND POSSIBLE TUBE BREAKAGE PROCEDURE cont 3 Perform a finger stick using good laboratory practice HELPFUL HINTS FOR COLLECTION OF CAPILLARY BLOOD SPECIMENS as outlined in the NCCLS publication Procedures for the Collection of Diagnostic Blood Specimens by Skin Puncture Second Edition Approved Standard NCCLS publication H4 A2 Villanova PA July 1986 CHOOSING A SITE 3a The site of choice in adults and older children would be the palmar finger print surface of the finger Do not use the side or tip of the finger Tissue thickness found in these locations is about half that found in the center of the finger Infants under the age of one year should have skin puncture procedures performed on either the lateral or medial plantar sur face of the heel or the plantar surface of a big toe This is to avoid injury to the infant from skin puncture of an insufficient tis
8. lary collection of whole blood SUMMARY The StatSampler requires assembly before use by inserting the Capillary tube the end with the colored band first through the split stopper See figure 1 Finger stick blood is collected into a Cap illary tube and then turned to the vertical position allowing the blood to drain into the MicroTube from which it can be used as a whole blood sample StatSampler blood collectors combine the advantages of 1 Immediate anticoagulation in the Capillary tube 2 Ease of collection in a Capillary tube 3 Reduction in technique dependency in collecting capillary blood CONTENTS 1 50 EDTA treated Capillary tubes 2 50 MicroTubes with attached stoppers 3 3 Pipette bulbs ANTICOAGULANT Each Capillary tube contains enough EDTA to anticoagulate the designated fill volume of blood There is no anticoagulant con tained in the MicroTube portion of the StatSampler If a volume of blood greater than the designated fill volume is required then a second Capillary tube must be used to ensure the proper antico agulant blood ratio STORAGE Keep vial of capillary tubes closed while not in use Store kit in a cool dry place USE BEFORE DATE StatSampler capillary tubes containing anticoagulant should not be used beyond the date stated on the vial label For in vitro diagnostic use PRODUCT DIFFERENCES SS1E Designated Fill Volume 100 uL SS2E Designated Fill Volume 200 uL PROCEDURE 1 Labe
9. ld be used if the specimen is obtained from an infant 4 This specially designed device delivers a cut that is only 1mm in depth preventing injury to the infant s heel bone and surrounding tissue Incisions between 0 35 and 1 6 mm in depth leave virtually no hematoma and allow for sub sequent sampling from the same region 4 8 Following collection StatSampler MicroTubes for serum prepa ration should be allowed to clot for at least 30 minutes before centrifugation Treated samples can be spun immediately to yield plasma Centrifugation e StatSpin centrifuges With StatSpin models RP or MP earlier models 1 amp 3 centrifuge with the RT12 rotor or equivalent using the Tube Rotor 30 seconds cycle to prepare plasma or serum e Low speed clinical centrifuges Centrifuge at 1500 rcf for 20 minutes The StatSampler MicroTube may be placed in a larger plastic centrifuge tube for easier access e High speed microcentrifuges Centrifuge for 2 minutes BARRIER GEL MicroTubes for plasma serum separation contain an inert barrier material which has a density between that of cells and plasma During centrifugation the inert barrier gel rises to the plasma cell interface where it lodges and forms a physical barrier between the liquid portion and cells This inhibits re mixing of the red cells with the plasma See figure 3 LIMITATIONS StatSampler has been designed for capillary blood collection Chemistry values obtained fro
10. lking the skin may cause hemoly sis and thus affect the accuracy of some determinations Some clotting may occur with non free flowing blood REFERENCES Hold the StatSampler assembly by the Capillary tube Collect the sample in the StatSampler taking care not to press the open ing of the Capillary tube to the skin After wiping off the first drop of blood aspirate blood into the Capillary tube as it appears at the wound The blood must be free flowing and not intermittent starting and stopping otherwise clots may form Tilt the tube horizontally or slightly upward Avoid introducing any air bubbles into the Capillary tube Fill the Capillary tube to the end if larger volume is desired use a fresh Capillary tube to maintain proper blood anticoagulant ratio 1 Procedures for the Collections of Diagnostic Blood Specimens by Skin Puncture Second Edition Approved Standard NCCLS publication H4 A3 Villanova PA July 1986 2 Blumenfeld T A Hertelendy W G and Ford S H Simultaneously obtained skin puncture serum skin puncture plasma and venous serum compared and effects of warming the skin before puncture Clin Chem 23 1705 1977 3 Meites Samuel Skin Puncture and Blood Collecting Technique for Infants Update and Problems Clin Chem 34 1890 1894 1988 4 Burns Edward R Development and Evaluation of a New Instrument for Safe Heel stick Sampling of Neonates Lab Med 20 481 483 July 1989 Stat Spin An IR
11. m skin puncture specimens may differ from those obtained by venipuncture 1 2 Milking the skin may cause hemolysis and thus affect the accuracy of some deter minations Capillary tubes treated with lithium heparin or EDTA have been wide ly used in the collection of blood and the subsequent prepa ration of plasma This product should perform in a similar manner However the user should determine that plasma of adequate quality for their testing purposes is produced by StatSampler Micro Blood Collectors i Figure 1 ka an SPECIMEN COLLECTION _ sr 3f After blood flow has begun wipe off the first drop Angle the _ if puncture site downward so as to facilitate blood flow Be certain not Frered to milk the appendage a steady firm pressure is preferable if re Plasma Z quired Gai P f Cells Figure 2 4 Hold the StatSampler assembly by the Capillary tube Collect the sample in the StatSampler taking care not to press the open Figure 3 ing of the Capillary tube to the skin After wiping off the first drop REFERENCES of blood aspirate blood into the Capillary tube as it appears at 1 Procedures for the Collections of Diagnostic Blood Specimens by Skin Puncture Second the wound Tilt the tube horizontally or slightly upward Avoid Edition Approved Standard NCCLS publication H4 A3 Villanova PA July 1986 introducing any air bubbles into the Capillary tube 2 Blumenfeld T A Hertelendy W G and Ford
12. sue depth area It is imperative that the depth of puncture in the infant not exceed 2 4 mm to avoid penetrating bone and the risk of osteomyelitis 3 The optimum puncture depth of the heel in the newborn is between 0 35 and 1 6 mm beneath the skin surface as this is where the major blood vessels are located 3 SITE PREPARATION 3c Warm the site Warming of the site drastically increases blood flow to the area resulting in a larger quantity of blood collected When following proper collection procedures up to 0 5 ml of blood can be obtained from a single puncture site Warming is achieved by either applying a warm compress to the site or plac ing the site under warm running water The site should be warmed at a temperature not to exceed 42 C for a minimum of three minutes Clean the puncture site Use a commercially prepared alcohol pad or a 75 aqueous solution of isopropanol After cleaning thoroughly dry the site with a sterile gauze pad Residual alcohol can cause hemolysis Do not cleanse area with betadine as it may cause an elevation of potassium phosphorus and uric acid Puncture the site A commercial lancet device either automated spring loaded or manual is available in a wide variety of styles for uniform depth punctures The Tenderfoot by I T C should be used if the specimen is obtained from an infant 4 This spe cially designed device delivers a cut that is only 1mm in depth preventing injury to the infant s he
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