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Sorvall WX+ Ultracentrifuge Series User Manual

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1. The centrifuge itself may move if the rotor fails during high speed rotation Make sure there is a 30 cm area around the centrifuge that will allow for such movement and do not allow individuals to enter into that area during operation Also do not place dangerous objects such as flammable or explosive materials on top of the centrifuge or in the surrounding area Section 3 2 and Chapter 6 1 Never open the door during rotation 2 Never touch the rotor during rotation Section 3 5 It can take more than three hours for the rotor to come to a complete stop because the rotor chamber is under vacuum Before opening the door wait until the rotor comes to a stop Section 3 5 Never touch the rotor while it is rotating Section 3 5 1 If the centrifuge rotor or an accessory is contaminated by samples that are toxic or radioactive or blood samples that are pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods 2 lf there is a possibility that the centrifuge rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the centrifuge rotor or the accessory properly before requesting repairs from_a Thermo Fisher Scientific authorized sales service representative J N SAFETY NOTICES 3 lt is your
2. o The user selected in the registered user display registration field 2 in the program registration screen appear o For details refer to Section 3 2 4 Logging in as a User 7 Press the white area in the The Remarks entry remarks display registration REMARK screen appears field 3 to enter a remark You can omit this operation a jl Space f elete Enter o Enter a remark and press the Enter button o You can enter a character string with up to 16 characters o Remarks entered in the remarks display registration field 3 in the program registration screen appear Select the color of the folder Eere you want to register in the aoti P display registration field 4 User nate Folder color display registration field o The selected folder is surrounded by a blue frame o Check the registered data and press the Next button 3 26 3 3 1 1 Program Registration Procedures Touchscreen operation screen displays and notes i i PROGRAM Wo 0008 Press the field in Step 1 Sr x check that the field turns STL Tew ACCEL oe cE aN a Ss blue and press the Edit A pE ee button EE EE f 4 2 FS fe a aaa HABE ody T i e E De eE str E is ___i__ Edit button ard iii a 10 Press the white area in the PROGRAM Nu 0008 STEP rx The run parameter entry display screen SPEED setting value display appears field 1 ncFave 7 i 1 SPEED setting value You can press
3. Indicates switch or button Open the door and install the rotor Close the door and set run parameters The set time is reached Or press STOP Select rotor and You can omit this The rotor stops user operation Press VACUUM Temperature control is turned off Vacuum pump is turned off Air leak The door lock is released Press VACUUM 1 The vacuum pump and temperature control are on 2 To separate samples that must not be exposed to temperature rises wait until a high vacuum level is reached Remove the rotor and turn the POWER switch off Press START The rotor starts turning You can start operation using START without having to press VACUUM Then the vacuum pump will start when START is pressed and the rotor will stabilize at 4 000 rom until the set vacuum level is obtained Fig 3 2 3 Operation Procedures 3 18 3 3 How to Use the Function Selection Area 3 3 How to Use the Function Selection Area This centrifuge provides programmed operation including step mode operation RCF display and setting function w T display and setting function and Real Time Control RTC that can run the centrifuge at a preset date and time and a host of other functions These features are displayed and controlled using the Function Selection Area in the Run screen 24 Oct 2013 9 30 _ Function Selection Area PROGRAN button SET 100 000 ROTOR NNS FUNCTION
4. The run parameter replaced by the OK button entry display screen Check the entered value and RCFave 599800 reappears press the OK button TEMP 10 0 Next Step button Register button o The TIME run time computed from the SPEED value and the wT value set in steps 17 and 18 is displayed in the TIME setting value field o To register step mode operations press the Next Step button and enter the next run parameter For details refer to Section 3 3 1 3 Step Mode Operation Procedures 1 Pressing the Register button when no numeric value is entered in the SPEED TIME TEMP ACCEL or DECEL field will generate an error and the field where the error occurred turns red An inappropriate setting value will also cause the field with the error to be displayed in red Example When SPEED and TIME are not set PROGRAM Wo 000a STEP 1 x RCF max RCFave 0 TIME HOLD 2 T TEMP 10 0 ACCEL 3 DECEL 5 2 Be sure to set the SPEED TIME TEMP ACCEL and DECEL run parameters Program registration and operation can be performed without entering RCFmax RCFavg and wT 3 RCFmax and RCFavg cannot be registered without installing a rotor 4 wT cannot be registered without setting SPEED 3 30 3 3 1 1 Program Registration Procedures Touchscreen operation screen displays and notes Check that the registered program has been added to the program list and press the X button Pre
5. 3 3 2 RCF Display and Setting Function 3 3 2 RCF Relative Centrifugal Force Display and Setting Function This centrifuge stores the maximum and average radii of each rotor in internal memory Setting a speed causes the centrifuge to automatically calculate and display the RCFmax value the maximum centrifugal force of each rotor and the RCFavg value the average centrifugal force of each rotor Likewise when an RCFmax value or RCFavg value is set the centrifuge will automatically calculate and display the speed The following is a description of how to display and set RCF 1 When the centrifuge calculates the RCF value from the set speed or calculates the set RCF value from the speed the numeric values may not be the same and deviate slightly 2 Some of the rotors available for this centrifuge have outside and inside tube cavities Refer to the instruction manual supplied with the rotor When rotors with both inside and outside tube cavities are used the instrument can calculate the speed and RCF value of the outer tube cavities Touchscreen operation Screen displays and notes Press the rotor indicator field in the Run screen If the desired rotor has not been set press the rotor indicator field to select the desired rotor ACCEL DEEL l l ACCEL g For information on how to japi RCF button select a rotor refer to Section Fail 3 2 3 Rotor Selection Rotor indicator Press the RCF button in the Fu
6. CEARN The selected Run History List screen for 23 Oct ta 152 Wann T 6100 l the selected user l appears EE p t 13 14 25 100 000 PP Selected user name 426 Sep 13 11 38 41000 TH G41 5 24 Sep 13 15 38 417 000 TH 641 o This allows you to check User History details as described in 1 Checking and Reusing Run History You can also use this function to reuse history data 3 62 3 4 1 Outputting and Reusing Run History Display and Run Parameters 2 3 Checking and Using Rotor Run History Touchscreen operation screen displays and notes Press the Menu button at the Tar recom The Menu box in Run bottom left in the Run History hath tet r SREI History appears screen 5 10 Oct 13 10 40 41000 TH 641 2 Press the Rotor Log button in the Menu box 1 Pressing the USB EXPORT button at the bottom left of the screen outputs the run history per rotor to a USB flash drive in the CSV file format 2 The centrifuge does not support secure USB flash drives 3 Press the cell of the rotor TOT Serer I L TAERE he selected Rotor Run whose information you want History List screen for to view g the selected rotor appears Selected rotor name 5 27 Sap 13 15 50 0000 T Sato In this example the run history for the T 8100 rotor is shown Page 0001 me aan es WD ena o This allows you to check User History details as described in 1 Checking and Reusing Run History You can also use this
7. DECEL ACCEL 9 oe i DECEL 9 NOTE 1 When the user lockout function described in Section 3 4 4 2 User Lockout is enabled you must login as a user to operate the centrifuge This requires a user to be registered as described in Section 3 4 6 1 User Management 2 The icon in the USER area in the Run screen differs depending on whether the user is logged in or not USER l Pmp Registered user name Icon for unregistered user Icon for registered user 3 2 5 Operation Procedures 3 2 5 Operation Procedures Below is a description of normal operating procedures AN CAUTION Take care not to get your hands or fingers caught in the door of the centrifuge NOTE Before you start using your centrifuge carefully read through the instruction manual for your rotor and make sure that the correct tube types and number of samples are supplied Touchscreen operation Centrifuge operations and notes Turn on the centrifuge o The touchscreen is displayed POWER switch o The door lock is released o When Economy mode see Section 3 4 5 9 Setting Economy Mode is engaged tap the touchscreen This disengages Economy mode Open the door install the rotor Gently install the rotor on the crown and close the door o Read through the rotor instruction manual Set the run parameters o Set the run parameters according to the instructions in Section 3 2 1 Setting run parameters and Section 3 2 2
8. STEPSAVERTOVG S N 0000001 i el MENU button Press the Delete Rotor FID SETSE TEC Sem A delete confirmation button in the menu box Peres message appears T 8100 S N 0000001 TH 641 S N 123456 Press the YES button in the Ma The selected rotor is f p Rotor Management delete confirmation box Nama ri deleted from the Rotor management screen TH 641 S N 1234567 return to the Run screen 1 Undefined 1 is the default selection that is automatically made when a rotor is installed without selecting Undefined 2 or another rotor from the rotor list It records the run history of all rotors that are installed in the same way 2 Select Undefined 2 rotor for a specific rotor whose run history you want to record without registering it 3 Undefined 1 rotor and undefined 2 rotor cannot be deleted 3 97 3 4 6 Admin Function 4 Run time indication 4s Select either the elapsed time or remaining time as run time Note that when the run time setting is set to HOLD elapsed time is automatically Lapse Remain selected Lapse Remain 1 Elapsed button saat Gta akan Elapsed time is displayed in the centrifuge run time tar tas ee rR indication field in the Run screen and Lapse is indicated to the right above the display field 2 Remaining button Remaining time is displayed in the centrifuge run time Sasa ore indication field in the Run screen and Remain is indicate
9. T value press the white area in the w T setting value display field 8 Operations up to step 18 are only required when a w T value is set The RCF setting screen appears Set rotor model SPEED display RCFavg setting value display field RCFmax setting value display field o When an RCFmax or RCFavg value is entered the other value is automatically computed and displayed o Enter the desired value and press the Enter button The RCFavg value is computed and displayed from the set RCFmax value SET 513 700 SPEED display OK Button o The SPEED value computed from the set RCF value and rotor information is displayed in the SPEED display NOTE If the SPEED value entered in step 11 differs from the SPEED value computed from the RCF value set here the value entered in step 11 is replaced with the computed value PROGRAM No 0008 STEP 1 x The run parameter ial a a reappears TIME 10 00 TEMP 10 0 ACCEL 5 i 3a Tan m DECEL 5 rT a To set a wT value SPEED must first be set This operation is not accepted if SPEED is not set 3 29 3 3 1 1 Program Registration Procedures Touchscreen operation screen displays and notes 18 Press the w T display area The w T setting and enter the desired value screen appears Example 27 12 wT 1 98x10 w T display Left area Mantissa Right area Exponent 19 The Enter button is PROGRAM Wi 0008 STEP x
10. Thermo Scientific Sorvall WX Ultracentrifuge Instruction Manual 50145792 a 09 2014 The Thermo Scientific Sorvall WX Ultracentrifuge is designed to separate liquid suspended materials General description having different densities and particle size The series is a series of products that pursue user friendliness and reliability based on our many years of experience in developing centrifuges This series offers many new features that we are confident of satisfying your requirements These features include the following BR OD Maximum speed is 100 000 rpm 803 000 x g WX100 The touchscreen with easy to see color liquid crystal screen is incorporated The displayed language can be switched over between English and Japanese Color touch sensitive liquid crystal display and graphic user interface with high contrast against back screen in black color enable you to easily operate the system or select various menus and functions by touching the icon on the display The real time control feature enables setting a start time or finish time thus letting you run your machine at desired date and time 6 Centrifugal force RCFmax and RCFavg can be displayed and set Note 7 Thousand varieties of 30 stepped mode can be programmed for a wide range of applications such as step running Various alert indication can notify users of the causes and necessary actions of the troubles It can realize trou
11. p a gt O O O p a Q a Oo prum 4 p an eb Q O For free coast press the FREE Press the on screen keypad to enter a numeric lt 2 3 4 complete press the Enter button Use the CE button to cancel incorrect entries Check the entered value Set it to 9 Set it to 7 To continue making other settings press the field of S the desired setting When ACCEL 1to9 0 Setting range 1to9 0 Free coast FREE 3 9 3 2 2 Acceleration and Deceleration Modes 3 2 2 Acceleration and Deceleration Modes In order to meet a wide variety of applications the acceleration and deceleration rates can be adjusted between 0 to 500 rpm By setting an ideal acceleration and deceleration time samples will not be disturbed which improves efficiency and reduces centrifugation time The table below shows the relationship between ACCEL and DECEL code numbers and acceleration and deceleration time Code Acceleration Deceleration from 0 Indicates ACCEL and N minutes from 0 minutes from DECEL code numbers i to 500 rpm 500 to 0 rpm Minimum time Minimum time Free coast 9 8 1 7 2 Speed 6 3 rpm 5 4 4 5 3 6 2 7 500 Pe gg te et ere a 1 8 0 9 0 FO gt Free coast from Time o _ stable speed 1 Minimum time The time it takes the drive motor to accelerate and decelerate the rotor using maximum torque This time will vary with rotor type and th
12. 6 Economy mode Green eae lighting 7 When accelerating to zonal speed normally Blue Slow blinking 3 000 rpm 8 When decelerating from zonal speed Slow blinking 9 When an alert occurs Rapid blinking 3 1 Method for setting display colors for status display lamp Touchscreen operation Instrument operations and notes Press the LED button es The LED indicator LED Indicator Setting setting screen wal S piee appears A BLINKT Set display color LED BLI TAU LIGHTING button 4 SL he ELINE IHG ILZS5LOW SAE BLUE BLINKING S PARSE oer as ey BLUE 3 79 3 4 5 Customizing Touchscreen operation Instrument operations and notes 2 Press the Set display color button for the operating condition you want to change Example To change the display color for When stopped from blue to white Select the desired color and brightness from the color palette screen press the Select display color button Then confirm that the selected button is surrounded by a white frame before pressing the SET button You must press the SET button to complete the setting Press the CUSTOM tab the X button the Run screen tab or the MENU tab hay SEE Ae The Color palette LED Indicator Setting screen appears Color Pallet STOP X button Select display color button LIGHT BLUE YELLOW B SS PURPLE None lt SET button o The buttons for selecting display color
13. Alconox is a registered trademark of Alconox Ficoll is a registered trademark of GE Healthcare Haemo Sol is a registered trademark of Haemo Sol Triton X 100 is a registered trademark of Sigma Aldrich Co LLC Valox is a registered trademark of General Electric Co All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Shown pictures within the manual are examples and may differ considering the set parameters and language United States Canada 1 866 984 3766 Netherlands 31 76 579 55 55 China 800 810 5118 or Latin America 1 866 984 3766 Nordic Baltic States CIS 358 9 329 10200 400 650 5118 Austria 43 1 801 400 Russia 7 812 703 42 15 Japan 81 3 5826 1616 Belgium 32 53 73 42 41 Spain Portugal 34 93 223 09 18 Other Asian Countries 852 2885 4613 France 33 2 2803 2180 Switzerland 41 44 454 12 22 Australia 61 39757 4300 ermo Germany national toll free 0800 1 536 376 UK Ireland 44 870 609 9203 New Zealand 64 9 980 6700 Germany international 49 61 84 90 6000 India 91 22 6716 2200 Countries not listed 49 6184 90 6000 or SCIENTIFIC Italy 39 02 95059 552 33 2 2803 2180 Part of Thermo Fisher Scientific 50145792 a original instruction manual published in 08 2014
14. En e RTC button ACCEL DECEL RCF wT ie D a_i Nec ets ACCEL 9 DECEL 9 w T button RCF button L Fig 3 3 Run screen You can save run parameters in memory for later use in repeated operation This feature also allows step mode operation that can combine a number of run parameters in a sequence of operations The centrifuge automatically computes and displays RCF values from set speed or speed from set RCF values This function is used to indicate w T a value obtained by adding run time to the angular velocity Squared the centrifugal effect Operation can be stopped when the set w T value is obtained sets a start time or end time and runs the centrifuge at a desired date and time The above features can be used in combination NOTE To combine PROGRAM and RTC first set PROGRAM and then RTC Once RTC is activated you cannot change the run time and set PROGRAM 3 19 3 3 1 Programmed Operation 3 3 1 Programmed Operation When a centrifugal condition will be used frequently entering the same condition every time you want to perform centrifugation is inconvenient This centrifuge has a programmed operation feature that stores run parameters Storing frequently used run parameters allows you to call those parameters when you need them which saves setup time The stored run parameters persist even when the POWER switch is turned Off As shown in the figure below the centrifuge
15. Schedule turns blue No User 1 Admin r 2013 10 28 11 30 kai 2013 10 28 18 00 Date yyyy ma dd HH MM 3 H Lung T reao 2701s T 18 00 Delete button o Use the lt and gt buttons to turn pages in the schedule list A delete confirmation message appears Schedule No User 1 Admin 2013 10 28 11 30 7013 10 28 18 00 Date yyyy ma dd HH MM Are you sure that you want to delete this schedule 3 H Lung 2013r 107 The User PIN field appears 3 7 3 4 5 Customizing Touchscreen operation Instrument operations and notes Enter the PIN of the user who s ssemeuSee The Schedule list i Schedule registered the schedule and No User Date vygv me dd WAW SC eer Eas press the Enter button 3013 10 28 11 30 2013 10 28 18 00 o The selected schedule is deleted from the schedule list o Press the CUSTOM tab the X button the Run screen tab or the MENU tab 3 Method for changing centrifuge schedules To change a schedule first delete the schedule according to the procedure described in 2 Method for canceling centrifuge schedules then reregister it on the desired date as described in 1 Method for registering centrifuge schedules 7 Date time setting Use this function to adjust the time of the internal clock RTC operation requires an accurate time setting Date Time Press the field of the item you want to set The selected field is displayed in red C
16. Should the rotor spinning at high speed fails or comes off the drive shaft the safety of the operator is ensured by the thick protective steel ring enclosing the bowl Fig 2 2 2 2 Imbalance detector If during operation the vibration of the rotor becomes excessive due to serious imbalance or improper bucket setting the imbalance detector detects the situation and decelerates the rotor immediately However the ultracentrifuge is designed to tolerate imbalance associated with visual balancing it is equipped with an imbalance tolerant drive For more information on the balancing of rotors see Section 3 1 2 Preparing tubes bottles and rotor 3 Door lock system The chamber door automatically locks for safety while the rotor is spinning When the power supply is off the door remains locked The door can only be opened and closed when the rotor is at rest and the rotor chamber is vented Unless the door is closed the rotor will not start rotating except in zonal mode To open the door in the event of a power failure see Section 3 5 Happenings when power failure occurs 4 Speed sensor and overspeed detector For protection in the event of entry errors the ultracentrifuge is provided with an automatic system to stop the rotor when its speed exceeds the maximum allowable speed If a speed higher than the maximum permitted speed is set the ultracentrifuge will detect the mistake before the speed reaches 3000 rpm and then will display a
17. Status display TK Use to set status displays LED indicator lamp For details refer to Section 3 4 5 8 LED Economy mode a Sets economy mode auto standby mode For details refer y to Section 3 4 5 9 Auto standby Press the icon for the function you want to use and select from the options that appear After completing the settings press the tab of the screen you want to return to for example the Run screen 1 Zoom display setting Select the zoom function to enlarge the speed and run time display in the Run screen display Zoom Setting Zoom 1 NORMAL button Select to display the normal Run screen 2 ZOOM button Select to enlarge the speed and run time display 20 seconds after reaching the set speed Fig 3 4 5 1 Zoom setting screen Press either the NORMAL or ZOOM button to as a RE qc 7 SPEED rpm select display mode Confirm that the selected button is surrounded by a green frame before pressing the CUSTOM tab a TET Laipec 100000 1 00 4 0 100000 k a SET r T 6100 nr SET or X button to store the setting man E ee oe OF 100 000 2 30 To switch from the zoom screen to the normal screen i Care a AER ss during operation press anywhere in the display area E2 cap 4 0 Lm 2 except the STOP button The zoom screen will then Normal screen Zoom screen automatically return to the normal screen in 20 seconds 2 Stop signal setting _ Select
18. and select a registered user Further operation is not possible without logging in as a user User access level determines what operations are permitted 3 90 3 4 6 Admin Function 1 Only a user logged in with Administrator privileges can enable the user lockout function 2 Use of the user lockout function requires that users are registered Register users as described in 1 User Management 3 When user lockout is enabled a user must be logged in to use the touchscreen 4 Once user lockout is enabled only a user with an Administrator access level can call the Admin screen To disable the user lockout function requires that a user having an administrator access level be logged in 5 In the factory defaults the user name Admin is registered as having an access level of Administrator and a PIN of 1111 6 The operations that users at each access level are permitted during user lockout are listed below Table 3 4 6 2 Operations at each access level CO O U U U OOU nro Run screen START STOP VACUUM button operations o o e koo i e weon o f o o RTC function ee ee po a Run History External USB output USB output External USB output o o xo Do x Run parameter reuse parameter reuse oag emee rr MENU Pa oo e Ce A Service Contact information Reference oe o o Zoom display seting e oo e o o Stopsignalseting ooo l e o oe o o o somavam S o S o o Dinning mode seting l o f gt
19. o cee a A E Register change andveferencescheduies o gt o gt Co S A situs aispiay ED marcator ampse S FEconomymodessting o f oe y o a e Customize O Enable x Disable 3 91 3 4 6 Admin Function 3 Rotor management Rotor Management You can register the rotor and its serial number in the instrument to use it in setting operations and control total run time and number of runs It is very important to manage the life of each rotor you use with the ultracentrifuge The warranted life of the rotor is different depending on the type of rotor the material the rotor is fabricated from and the policy of the manufacturer of the rotor The warranted life of a Thermo Ultracentrifuge rotor is defined by a maximum number of runs Other manufacturers use a combination of either the maximum number of runs or a maximum number of hours to limit the warranted life of the rotor For some rotors there exists a primary and a secondary life The primary life is defined as the initial usage at speeds up to the maximum rated speed of the rotor Once this primary life is expended the rotor has a secondary life at soeeds up to a derated maximum speed of the rotor typically 90 of the initial maximum rated speed 1 Rotor life management In order to comply with warranty requirements rotor use must be documented in a rotor log book provided with the ultracentrifuge Additionally the Sorvall WX Ultra series centrifuges have t
20. screen If they are correct press the X button and press the X button in the Program registration screen Check that the folder you just registered has been added to and is displayed in the Program list screen before pressing the X button 7 Check the entries displayed for each step in the run The run parameter entry display screen in STEP 3 appears PROGRAM No 0010 STEP 3 SPEED 60 000 RCF max 288 700 RCFave 215 900 Register button o Like in STEP 1 the value entered in STEP 2 is copied to the run parameter entry display screen PROGRAM fo 0010 SPEED TIME iy ice TAE y 0 iam VE 80 000 1 00 EIE E The run parameter setting display screen aS ea reappears ae E ee as a ee 60000 0 30 a rR Rip ai aw S e HH 3 fs _ ie _ f 5 m S X button o The entered values are displayed in the STEP 1 2 and 3 fields o To correct entry errors press the STEP field you want to correct and press the Edit button when the field turns blue The Program list screen reappears PROGRAM 0001 noo me i i D Ea 5 ath ies Lung Admin ogar ie E h 6 oI dimei m biai eo a X button erali The program registered in this example o The Run screen reappears 3 43 3 3 1 3 Step Mode Operation Procedures _ 2 Step Mode Operation Procedures Call the number of t
21. w T button turns blue 3 To cancel w T operation change the speed or run time setting 3 53 3 3 4 RTC Real Time Control Operation 3 3 4 RTC Real Time Control Operation This centrifuge contains an internal clock allowing you to operate the centrifuge between a set start time and end time This function for operating the centrifuge between set time intervals is called Real Time Control RTC The RTC operation procedures are described using examples below Example To operate the centrifuge under the following run parameters starting from rotor installation on October 24 and removing the samples around 9 30 the next morning Rotor T 8100 Speed 100 000 rom Separation time 3 hours 1 2 3 4 5 6 Control temperature 4 C Acceleration mode 9 Deceleration mode 9 SET ie ii eer lili Trl t i NN 3h 100 000 preteen veers e ser START button ON l Speed rpm October 24 PM 4 30 October 25 AM 6 30 Around 9 34 Auto start Stop End time AM9 30 Fig 3 3 4 RTC Operation Example In this example set above run parameters 1 to 6 then set the RTC operation start time to 6 30 October 25 and start the centrifuge Note that setting an end time of 9 30 instead of the start time 6 30 will perform the same operation 3 54 3 3 4 RTC Real Time Control Operation Touchscreen operation screen displays and notes Set run parameters according er 24 Oct 2013 16 30 ihe s
22. 192 080 press the Enter button FRG E451 9 50 000 F37L 8x100 37 000 T 81 00 00 gog To confirm rotor details press the rotor field to turn it blue and press the Details button Rotor Name Number of Tubes Tube volume ml Max sSpeeadi rpom A window that allows you to check rotor information appears Rmax temi Ravgtom RCFmax xg RCFavg iym If this is the selected rotor press the Enter button Design dens g ml To select a different rotor press the X button to return to the Select rotor screen 3 12 The pressed field turns from white to blue 182 460 128 029 a02 000 598 B29 The Rotor Details screen appears a 6 50 100 000 7 18 5 37 a0 006 599 29 c 1 20 P _ e_ 3 2 3 Rotor Selection Touchscreen operation screen displays and notes 5 The Run screen appears and EPUA PERE The Run screen the name of the selected kara If ane hh TEMP c appears rotor type is displayed in the Rotor indicator field Rotor indicator field ACCEL DECEL ACCEL DECEL 9 1 NOTE 1 You cannot perform life management of a rotor just by selecting a rotor in the ROTOR CATALOG screen You must first register your rotor in the instrument Refer to Section 3 4 6 3 Rotor Management 2 By registering your rotor in the instrument in advance it appears in the Select rotor screen simplifying operation 3 13 3 2 4 Lo
23. 8 34 Alert message ACCEL I DECEL L 9 ACCE DECEL 9 Fig 5 1 Displaying an alert message If an alert message appears remove the cause of the trouble as described below and press the CE button You will then be able to resume your centrifugation AN WARNING Unspecified repairs remodeling or disassembly of the centrifuge that is not listed in Table 5 1 is strictly prohibited by any person other than a Thermo Fisher Scientific authorized sales service representative If the alert message persists even after you have done what is specified below contact service representative to order a repair Table 5 1 Alert List e Unless the set run time has elapsed restart the run e If the instrument was automatically restored and the rotor is rotating at set speed then let the run continue 1 A power failure occurred while the POWER FAILURE rotor was rotating Refer also to section 3 5 Happenings when power failure occurs INVALID SPEED 1 Rotor speed is set higher than the e Set the speed within their permitted maximum allowable speed limits 1 Rotor is not properly balanced and Check if the sample tubes exceed abnormal vibration has occurred in allowable imbalance level e Check if any one of the tubes is deformed and if there is any sign of 2 Rotor cover or cap is not properly sample leakage IMBALANCE tightened 3 The ACCEL DECEL code numbers Tighten rotor cover or cap secur
24. C 3 71 3 4 5 Customizing 3 4 5 Customizing SV This function allows you to adjust Run screen displays the stop signal sound volume screen backlight brightness and other functions to your preferences Customize Press the Customize button in the MENU screen to open the CUSTOM screen with the icons for setting and changing zoom display the stop signal and other functions shown in Fig 3 4 5 da D Stop Signal Volume Backlight RS EA nes gt hEDA Japanese Senedule Date Time LED EE 4 Auto standby Fig 3 4 5 CUSTOM screen The functions of the CUSTOM screen icons are described below Name Icon Function Zoom Allows you to change Run screen display appearance For details refer to Section 3 4 5 1 This function provides a selection of six sounds including 5 Stop signal tunes and an electric beep For details refer to Section 3 4 5 2 0 Sauna volume Adjusts the sound volume of the stop signal For details refer to Section 3 4 5 3 Back light Adjusts the screen backlight For details refer to Section 3 4 5 4 Display Switches between Japanese and English language For details refer to Section 3 4 5 5 Schedule Enables registration of a centrifuge schedule For details refer to Section 3 4 5 6 Schedule 3 72 3 4 5 Customizing Name Icon Function Use for making date and time settings For details refer to Section 3 4 5 7 Date Time Date Time
25. Emea Perey The ROTOR ROTOR CATALOG x CATALOG screen Fean ngle SWIRE Bucket appears Rotor ator Continuous Flow J Neo Anele Rotor w Rotor In the ROTOR CATALOG TL Em TETN Merete The Rotor list screen screen select the desired meses sk hd Seine ernie Shas Eg appears rotor type STEPSAVERTUVE 70 000 457 420 431 296 SSTEPSAVER65V19 65000 400 671 361 972 STEPSAVERSOV39 50000 pa 831 RRA 7 TV 1605 65 000 4 357 383 209 TV 865 69 000 400 677 369 523 Em lt aA _ __ Enter button Details button Select a rotor to register un sa ri eee The Serial number Check that the field turns blue S N field appears and press the Enter button Select a rotor press the Details button to open the Rotor details screen to check rotor specifications 3 4 6 Admin Function Touchscreen operation screen displays and notes Enter the serial number S N iam eri FTE eee The Rotor and press the Enter button ihs Management screen appears Name STEPSAVERTOVE S N 0000001 o The added rotor and its serial number are displayed o An M for manual appears at the right end of the field for the added rotor indicating that it is a rotor with an overspeed decal 2 Procedures for registering a rotor that has been used The following example describes how to register a rotor that has been used in another centrifuge In the registration the Run history so far total run
26. Press the cell of the user UT SERESD TEL The User PIN field whose schedule you want to appears register 4 Enter the PIN of the selected SEE TE The Schedule date time user and press the Enter i a a T entry screen appears button Start time T End time Press the cell you are interested in and enter the desired date and time on the on screen keypad Enter the desired schedule ase AN e The Schedule list Schedule time and press the Enter TTR he ee SCreen appears 1 Admin button o 2013 10 28 11 30 2013 10 28 18 00 2013 117 5 09 30 2013 11 6 16 00 E Added and registered 2013 177 T 11 30 MaI 7 schedule o The Schedule list shows registered schedules o Press the X button the CUSTOM tab the Run screen tab or the MENU tab 3 76 3 4 5 Customizing 2 Method for canceling centrifuge schedules Step Touchscreen operation Instrument operations and notes 1 Press the Schedule button MA Press the schedule cell you want to delete 2 3 Check the schedule details of the cell that turned blue and press the Delete button A Press the YES button to delete or NO to cancel The CUSTOM screen is replaced by the Schedule screen Schedule No User 1 Admin 2013 10 28 11 30 7013 10 28 18 00 Date yyyy ma dd HH MM 2 1 Sata 2013 117 5 09 30 2013 117 amp 16 00 3 H Lung 2013 11 7 7 11 30 20137 11 7T 1 00 EPA The selected cell
27. are shown in 3 different levels of brightness o The selected color blue and brightness intermediate are surrounded by a white frame fun screen NEn The LED indicator LED Indicator Setting setting screen LIGHTING appears Lighting test button HET ELINKING m Set display color A St button BLINKING py El stow BLUE o The Set display color button assumes the qualities selected in the color palette screen white and intermediate brightness o The Lighting test button to the right changes to an object in a double orange colored frame and the status display lamp LED indicator is test displayed in the selected color o Press the CUSTOM tab or the X button to return to the CUSTOM screen o Press the Run screen tab or MENU tab to return to the respective screen Eaa If an alert color and pattern are selected for something other than an alert a message appears notifying that such a choice will make it more difficult to differentiate it from an alert It is recommended that you use a different color or pattern to distinguish it from an alert 2 When a selected color or pattern combination is already used for another display color in a change of an alert display color or pattern a message appears notifying that the selected color is already used Like in case 1 it is recommended that you use a different color or pattern 3 80 3 4 5 Customizing 3 2 Method for setting display pat
28. dim the backlight only when the rotor is not turning and Fig 3 4 5 4c Screen for setting the pressing Dimming even while operation will dim the time period after which the backlight lighting after the set time period also during operation will be dimmed 5 Language switch button Press this icon to switch the display language between Japanese and English Japanese 6 Schedule Y4 This centrifuge allows you to record register up to 40 schedules The following describes how to record register a schedule Note that a user registration for details refer to Section 3 4 6 1 User Management is required for this operation Schedule 1 Method for registering centrifuge schedules Touchscreen operation Instrument operations and notes Press the Schedule button tat The CUSTOM Schedule No User Date ETET v ma di HH MM screen is replaced LA TTE l by the Schedule 2013 10 28 11 30 2013 10 28 18 00 z screen Ts 20187117 5 08 30 2013 117 amp 16 00 Schedule Schedule Add button Press the Add button utsen TIS The User list User Management x User name Access level Last run sl nla 23 Oct 2013 15 45 ard aot 2013 13 20 User cell P_Johasean Administrator 15 Oet 2013 9 15 H Lung Supervisor Ta Jun 2013 14 00 o Use the lt and gt buttons to turn pages in the user list 3 75 3 4 5 Customizing Touchscreen operation Instrument operations and notes 3
29. displayed in the selected pattern o Press the CUSTOM tab or the X button to return to the CUSTOM screen o Press the Run screen tab or MENU tab to return to the respective screen If Steady lighting is selected for a display pattern in Auto standby lighting will continue even if a transition is made to economy mode for details refer to 3 4 5 9 Setting economy mode To turn off the LED indicator in economy mode select NONE in the Color palette screen 3 81 3 4 5 Customizing 9 Economy mode setting and the cooling fan will stop turning to reduce power consumption when the ai When economy mode is enabled the touchscreen backlight will automatically dim centrifuge has been left idle for a preset period of time Auto standby 1 Method for engaging economy mode Touchscreen operation Instrument operations and notes Press the Auto standby En Te The Set auto standby button Auto standby setting screen appears a 5 ya Auto standby mst Disable button Enable button Press the Enable button to FUN Sereen Merit The Enable button engage economy mode Auto standby setting is surrounded by a green frame and the time until the eee ECE economy mode is ia iz enaaaed is indicated Enable Disable nee S O M Waiting time ai indication field Check time left until economy jemne The Set auto Auto Standby timeout setting standby waiting time mode
30. floor transmitting external vibrations to the ultracentrifuge 2 Ambient temperature for operation is 2 to 40 C If the room temperature rises above 30 C the temperature of the rotor may become too high Avoid installing the ultracentrifuge in direct sunlight or installing it at the place whose ambient temperature is higher than 30 C 6 1 6 Installation 3 The ultracentrifuge requires a clearance of more than 20 cm at its rear and a floor space of at least 90 x 90 cm See Fig 6 1 Ensure the surrounding of the instrument has a free circulation of air Avoid installing the ultracentrifuge near any other heat generating machine equipment which might reduce the ultracentrifuge cooling capacity Safety cover 20 cm or more 90 cm or more 90 cm or more Concrete floor etc Sg Fig 6 1 Place of installation J WARNING The centrifuge itself may move if the rotor fails during high speed rotation Make sure there is a 30 cm area around the centrifuge that will allow for such movement and do not allow individuals to enter into that area during operation Also do not place dangerous objects such as flammable or explosive materials on top of the centrifuge or in the surrounding area 6 2 6 Installation 3 Fixing the safety cover The ultracentrifuge is shipped with the safety cover removed from its rear When installing the ultracentrifuge remove the packing and insert the lower part of the safety cover into the two ho
31. follow below when servicing the centrifuge 1 Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord Then wait at least three minutes and then remove the covers from the centrifuge 2 Be sure to turn off the POWER switch and unplug the power cord from the receptacle if your centrifuge is equipped with a power cord with a plug Then wait at least three minutes and then remove the covers from the centrifuge Section 3 5 Chapter 4 Chapter 5 and Chapter 6 2 Indication of N WARNING 1 The overspeed decal must match the maximum speed of the rotor because the overspeed decal is a critical component that detects the over speed of the rotor If an overspeed decal that is not compatible with the rotor is attached the rotor could break resulting in damage to the ultracentrifuge Section 2 2 4 1 This centrifuge is not explosion proof centrifuge Never use explosive or flammable samples or materials that chemically react vigorously Do not centrifuge such materials in this instrument nor handle or store them near the instrument 2 Take all necessary safety measures before using samples that are toxic or radioactive or blood samples that are pathogenic or infectious Section 3 1 Do not incline or move the instrument while the rotor is spinning Do not place any object on the instrument or lean on the instrument Section 3 2
32. has a program area that can store 1 000 memory items each of which can hold 30 step conditions By storing multiple steps in a memory unit you can change the speed run time temperature and other parameters during operation Memory unit 1 Memory unit 2 Memory unit 3 Memory unit 1 000 Fig 3 3 1 Program area 3 20 3 3 1 Programmed Operation Basic operation of the programmed perc feature f7 SPEED rpm ay SA SET 100000 ROTOR _ FUNCTION NG REF wT oOo LC ACCEL DECEL OCEL 9 DECEL 9 Program display screen r Wa ACCEL DECEL ACCEL 8 DECEL 9 Folder button If the registered program No is not known Press the Folder button The Program list screen appears PROGRAM F Sato Admin ma H Lung Select a program folder check that the folder turns blue before pressing the Select button Use the lt lt lt gt and m buttons to turn pages Press the PROGRAM button 24 Oct 2013 9 30 STOP The Function Selection Area changes to the Program display screen N PROGRAM No button If the registered program No is known Press the PROGRAM No button The Program No entry screen appears PROGRAM Use the on screen keypad to enter the number of the desired program and press the Enter button 3 21 3 3 1 Programmed Operation 24 Ost 2013 9 30 As
33. ical or radioactive contamination may occur Always be aware of this possibility and take normal precautions Use appropriate decontamination procedures should exposure occur Service Decontamination If a centrifuge or rotor that has been used with radioactive or pathogenic material requires servicing by Thermo personnel either at the customer s laboratory or at a Thermo facility comply with the following procedure to ensure the safety of all personnel f Clean the centrifuge to be serviced of all encrusted material and decontaminate see Maintenance Section of centrifuge it prior to servicing by the Thermo representative or returning it to the Thermo facility There must be no radioactivity detectable by survey equipment The Sorvall Product Guide contains descriptions of commonly used decontamination methods and a chart showing method compatibility with various materials The Care and Maintenance Section of this instruction manual contains specific guidance about cleaning and decontamination methods appropriate for the product it describes Clean and decontaminate your centrifuge as follows a Remove rotor from the rotor chamber b Decontaminate door and rotor chamber using an appropriate method Complete and attach Decontamination Information Certificate in the back of your rotor or instrument manual to the centrifuge before servicing or return to Thermo facility If Certificate is not available attach a written st
34. is displayed in number or the wrong value for a run parameter lace of Hold place of Hold Enter Press this to save the entered setting X Press this to close the keypad display External connection Name and symbol Functions and actions USB host side Use the USB connection to output the operation history of the O centrifuge to a USB flash drive USB device side Use for connecting the provided himac ASSIST or use for maintenance LAN Use for connecting the himac LogManager option O amp I 2 5 2 2 2 Rotor Chamber 2 2 2 Rotor Chamber The structure of the rotor chamber vacuum chamber is shown in Fig 2 2 2 Bowl Rotor Chamber Drive shaft crown Handle Chamber door seal Chamber door Speed sensor Overspeed detector detecting any instance exceeding the maximum allowable speed of the rotor Door lock system Vacuum chamber Temperature sensor measuring Protective steeling ring the temperature of the rotor Thermoelectric cooling element cooling the rotor Fig 2 2 2 Rotor chamber If sample or water drops to the window of the temperature sensor it may cause an incorrect detection Whenever the sensor is wet wipe it with a clean dry cloth Take care not to scratch the surface of the sensor 2 2 3 Safety Devices 2 2 3 Safety Devices 1 Protection of rotor chamber
35. is used for sterilization no guarantee of Sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use For information on the maintenance of rotors and tubes see rotor instruction manual 4 1 Rotor Chamber AN CAUTION Do not pour any solution such as water detergent and disinfectant directly into the rotor chamber Otherwise the bearings of the drive unit may be corroded or deteriorated To maintain the rotor chamber follow the instructions given below 1 When the ultracentrifuge is not in use keep the rotor chamber ventilated 2 If the bowl is moist wipe it with a clean dry cloth or sponge 3 If the rotor chamber is dirty wipe it with a clean cloth or sponge dampened with a diluted solution of mild non alkaline detergent While doing this be careful not to touch the window of the temperature sensor 4 If the door seal o ring is dusty or scratched high vacuum level will not be obtainable Always keep the door seal o ring clean When the ultracentrifuge is used frequently take out the door seal o ring and wipe it with a clean soft cloth and then put a light coat of vacuum grease on it every three to four months ordinarily once a year If the door seal o ring is damaged replace it Wipe the groove for the door seal o ring with a clean soft cloth dampened with alcohol or a similar solvent 4 2 Drive Shaft
36. manage user run histories or control user Management operation 1 Method for registering users Touchscreen operation Screen displays and notes Press the User Zee The Admin screen i W nt Management button ote Access level Last run replaces the user Administrator 23 Oct 2013 15 45 management P J 7 7E screen 02 Get 2013 13 20 P Johnson Administrator 15 Oet 2013 9 15 User Management l Superul sor 1B Jun 2013 14 00 ee Add user button Press the Add User button TES Ei Pees The Register user User name x name screen Sa N appears TERE EeEre User name field Er ECRAOCnEE AWS ed of ey a a a 3 85 3 4 6 Admin Function Touchscreen operation screen displays and notes o The buttons in the Register user name screen have the following functions Shift button Press to switch from lower case to upper case letters Clear button Deletes all characters in the User name field Space button Enters a space at the rightmost position in the User name field Delete button Deletes the character in the User name field starting from the rightmost position Enter button Press to register the entered user name The Register PIN screen appears X button Press to return to the User management screen Enter the name of the user Laem The user entered in you want to register User nane Ef the User name field is EEE re eee of olf eH mf tl fd fs df ff if mi i el ee Enter bu
37. modes with long acceleration times which could cause an imbalance alert to light even within tolerable imbalance values 3 10 3 2 3 Rotor Selection 3 2 3 Rotor Selection This centrifuge stores the maximum and average radius of each rotor in internal memory It provides a function that will automatically calculate and display the relative centrifugal force RCF from a set speed and calculate and display the speed from an RCF setting for information refer to section 3 3 2 RCF display and setting function A correctly selected rotor allows you to manage total run time and number of runs Rotor selecting procedure Touchscreen operation Screen displays and notes 1 Press the Rotor indicator field penu Saeed The Rotor in the Run screen AE AE VEENI x Management ROTOR eee Undefinel screen T3100 S N 0000001 TH 641 S N 1234567 A rotor is selected when the Cancel name of a rotor type is button displayed in the Rotor Roto indicator field If you want to use another type of rotor follow the procedure below to select a different rotor 2 Check that the desired rotor is o When the selected rotor field turns blue press the OK displayed If it is select it and button to return to the Run screen press the OK button o The selected rotor type appears in the Rotor indicator field You can select your rotor in the Rotor Management screen if you registered your rotor in advance For inf
38. more than one level of protection shall be provided in the case of materials of a higher group e f the centrifuge rotor or an accessory is contaminated by samples that are toxic or radioactive or blood samples that are pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods e f there is a possibility that the centrifuge rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the centrifuge rotor or the accessory properly before requesting repairs from a Thermo Fisher Scientific authorized sales service representative e It is your responsibility to sterilize and or decontaminate the centrifuge rotor or parts properly before returning them toa Thermo Fisher Scientific authorized sales service representative Notice for an Earthquake Depending on the magnitude an earthquake might damage centrifuge If you observe some abnormality stop using the centrifuge immediately and ask for inspection by the Thermo Fisher Scientific service representative J N SAFETY NOTICES A Precaution indications in this Manual The followings informations indicate the precaution indications and the chapters sections which mention them in this manual 1 Indication of A DANGER To avoid electrical shock hazards
39. other data can be stored as memos ID Contact Touchscreen operation screen displays and notes Press the ID Contact renee i oem The instrument ID button xX service contact Machine ID display setting screen Gaza appears Service contact ID Contact Press the Instrument ID ruin screen MEMA Peo The instrument ID entry screen appears se eee EZEecCeecerre ea e ed yy e oe REEE TEUT M EUERTECEEE Belete Enter o Enter an alphanumeric character string and press the Enter button The instrument ID and the service contact display setting screen reappear showing the entered ID in the instrument ID field o You can enter a character string with up to 6 characters ied The Service contact field appears Service contact fe EEE ce eceeee 2 ea TH el HO CECCECECE me RAEC gf ff lf oll mp GEN rer i So o Enter a memo for the contact address of the authorized service center and press the Enter button The instrument ID and the service contact display setting screen reappear displaying the entered memo o You can enter a character string with up to 30 characters 3 101 3 4 7 Instrument ID Service Contact Information Touchscreen operation screen displays and notes Press the MENU tab X o Press the MENU tab or the X button to return to the MENU screen o Press the Run screen tab to display the Run screen button or the Run screen tab 3 102 3 5 Happenings when Power Failure Occ
40. replacing the overspeed decal with a new one be very careful not to damage the overspeed decal and rotor body Section 2 2 4 Do not place containers holding liquid in the rotor chamber on the centrifuge or near the centrifuge Liquid if spilt could get into the instrument and damage electrical and mechanical components Section 3 1 1 Do not press the touchscreen with a sharp pointed object such as a ballpoint pen 2 If abnormal sound or vibration occurs stop the operation immediately and contact _a Thermo Fisher Scientific authorized sales service representative Section 3 2 If you have selected a rotor in the Rotor Management screen be sure to check that the selected rotor in the Rotor Management screen and the rotor that is installed in the centrifuge have an identical serial number The rotor serial number is also displayed in the Rotor indicator field in the Run screen If the serial number of the selected rotor differs from the serial number of the rotor installed in the centrifuge you cannot control total run time and the total number of runs of both rotors Section 3 2 3 Take care not to get your hands or fingers caught in the door of the centrifuge Section 3 2 5 vi J N SAFETY NOTICES Zonal centrifugation includes operations where the rotor will turn while the door is open Be sure to read though the Zonal rotor instruction manual before operation Section 3 4 3 The cap is installed by hand o
41. such as a ballpoint pen e Be sure to remove the rotor from the rotor chamber when centrifuge is not used for a long time or when the machine is moved Otherwise the drive shaft crown may be damaged o Safety during installation and maintenance N DANGER WARNING e To avoid electrical shock hazards follow below when servicing the centrifuge 1 Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord Then wait at least three minutes and then remove the covers from the centrifuge 2 Be sure to turn off the POWER switch and unplug the power cord from the receptacle if your centrifuge is equipped with a power cord with a plug Then wait at least three minutes and then remove the covers from the centrifuge e When a power failure occurs during operation it takes 3 hours or more for the running rotor to stop completely because the rotor chamber is depressurized and has less air to stop the rotor Be sure to leave sufficient time before opening the door of the rotor chamber e For maintenance and repairing of the rotors tubes etc see the rotor instruction manual and the rotor tube bottle and cap instruction manual e After installation and before any test run this ultracentrifuge always needs the internal check by a Thermo Fisher Scientific authorized sales service representative e Repairs disassembly and other modifica
42. the the previous page MENU screen or the Run screen Use the MENU tab to return to the previous MENU screen Use the Run screen tab to return to the Run screen 3 65 3 4 3 Zonal Operation Procedures 3 4 3 Zonal Operation Procedures Zonal operation uses a zonal rotor for NORMAL density gradient centrifugation to ZONAL efficiently process large volume samples ZONAL Zonal operation involves the following three zonal modes Setting 1 With the door opened the rotor turns at low speed zonal speed to allow loading of samples 2 Samples are brought up to set speed Time high speed for separation 3 The rotor decelerates to zonal speed the air leak valve opens to vent the rotor chamber to enable opening the door and removing the samples Zonal speed Fig 3 4 3 Zonal Operation Modes Zonal speed A rotor speed used when loading and unloading samples Usually the speed is set to 3 000 rpm but to enable an optimum speed setting it is possible to set a speed between 2 000 and 3 000 rpm in 100 rpm increments For information on methods for changing run speeds refer to Section 3 4 6 7 N CAUTION Zonal centrifugation includes operations where the rotor will turn while the door is open Be sure to read through the Zonal rotor instruction manual before operation When zonal operation is set zonal specific temperature control temperature control for unpainted rotors should be used U
43. the Run screen appears the registered run parameters are set i iein _ The program No is displayed ieee i step 1 1 in the Folder button in the AEO EE Se Program display screen ACCEL 3 i Eoc Gast 8 _ 7 When multiple steps are registered the step operation display indicates the total number of steps and the current step Press the START button Ka 1 Programs cannot be registered changed or deleted during operation Perform these operations when the centrifuge is not running 2 When the screen is closed by pressing the X button in the program display screen after calling a program the Function Selection Area appears again but program operation remains enabled The Program button changes to blue to indicate that the program is still enabled 3 If the POWER switch is turned off when the Program display screen is open the Program display screen will be closed when the centrifuge is restarted and the Function Selection Area will appear However the program will still remain enabled and the Program button will turn light blue as stated in 2 above 4 Pressing the START button when the Program button is lit blue and a program is enabled will start the program and the Function Selection Area is replaced by the program display screen 5 To cancel called program operation call another program or change run parameters 3 22 3 3 1 1 Program Registration Procedures 3 3 1 1 Progr
44. the rotor stops turning o Process the samples as necessary press the VACUUM button to return the rotor chamber to normal atmospheric pressure open the door and remove the rotor Install the rotor again and close ermine The Program display the door before pressing the S screen in the Run START button en screen proceeds to step 3 When processing samples that are sensitive to aN temperature rises press the eee VACUUN button to obtain a oe high vacuum level in the rotor chamber before pressing the 22 START button o Astep where speed is set to 0 rom the user must press the START button to proceed to the next step of processing After performing the required processing install the rotor close the door and be sure to press the START button Repeat these operations as required NOTE 1 If the run parameters are changed during step operation and the 0 rom step the program will be canceled and no further steps will be processed 2 When the rotor is removed in performing a 0 rom step close the door of the rotor chamber until it is installed again to prevent unnecessary moisture buildup in the rotor chamber 3 In step mode operation that includes stops the run time calculation of all steps does not include the run time of 0 rom steps Step mode operation cannot be combined with RTC Real Time Control operation refer to Section 3 3 4 RTC Real Time Control operation 3 48
45. user and remarks select a folder color and press the Next button In this example the steps are Next button Edit button stored in program No 10 Press a field in Step 1 check CEST eS ee fx The run parameter that the field turns blue and seo i Ca screen press the Edit button Pp Next Step button wie oP tlie Enter the run parameters for Eee teins The run parameter step 1 from the run ore PRA entry display screen parameter entry display appears screen TEMP 4 0 STEP 1 SPEED 100 000 rpm TIME 1h30min Next Step button TEMP 40 C Stet ACCEL 9 DECEL 9 o Registering a rotor will make it possible to compute and ae RCFmax and RCFavg values from the set SPEED value Press the Next Step button The run parameter and enter the run parameters entry display screen for step 2 from the run in STEP 2 appears parameter entry display screen for step 2 STEP 2 g SPEED 80 000 rpm Entry screen STEP No TIME 1h M ACCEL 9 DECEL 9 o The value entered in STEP 1 is copied to the run parameter entry display screen 3 42 3 3 1 3 Step Mode Operation Procedures Touchscreen operation screen displays and notes 5 Press the Next Step button and enter the run parameters for step 3 from the run parameter entry display screen for step 3 STEP 2 SPEED 60 000 rpm TIME 30 min TEMP 4 0 C ACCEL 9 DECEL 7 Press the Register button parameter setting display
46. 01 Menu button Insert a USB flash drive into O This outputs the recorded run history to external media in the USB host port and press the CSV file format the Export Run history in o For details on inserting a USB flash drive refer to Section 2 2 1 Touchscreen and External Connections the MENU box o No special procedure is required to remove a USB flash drive Remove the flash drive when loading is complete The centrifuge does not support secure USB flash drives 3 61 3 4 1 Outputting and Reusing Run History Display and Run Parameters 2 2 Checking and Using User Run History Touchscreen operation Screen displays and notes Press the Menu button at the bottom left in the Run History screen 2 Press the User Log button in the Menu box 3 Press the cell of the user whose information you want to view In this example the run history for a user with the Admin user name is shown TEAREN he Menu box in Run aes History appears No i i Manu 5 10 ct 13 10 40 47 000 e Page 0001 SETE c e ES Mica Raa Fur sores IEPs N Teena he User Run History User Log TOTAL REV x screen appears oe Oe Usar name User cells P_Johasean H Lung 1 Pressing the USB EXPORT button at the bottom left of the screen outputs the total operation results per user to a USB flash drive in the CSV file format 2 The centrifuge does not support secure USB flash drives
47. 3 ra Folder button Press the Folder button in SROGRAU The Run screen replaces the the Program display screen ae wall p ist p l ma A j M rogram list screen 0004 0005 ALL button im g4 I Lung l e Differently colored me Po amp s folder buttons RNA Admin w T m CF o a re ae MENU button a If the color or design of the o Pressing a color or design button will display only the registered folder is known program registered to that color design press the button with that o Pressing the ALL button will display all folders regardless of their color or design in order color or design o Use the lt lt lt gt and gt gt buttons to turn pages b If the color or design of the registered folder is not known press the ALL button a If the Folder button indication makes clear which program folder you want to call press that C ser 4 30 Arun parameter called Folder button Check that E Progra step 178 EA from the program 000000 eee The display again shows the Run screen the periphery of the folder REET DETETA turns blue and press the Select button Arun parameter called from the program 3 39 3 3 1 2 Programmed Operation Procedures Touchscreen operation screen displays and notes b 1 To confirm registered PROGRAM The MENU box appears 0003 data press the Folder me Pe a in the Program list f T Sato Admin
48. Acceleration and Deceleration Modes 4 Select a rotor o Select a rotor according to the instructions in Section 3 2 3 Rotor Selection You can skip this step 5 Login as a user o To login as a user follow the instructions in Section 3 2 4 Logging in as a User You can skip this step Press the VACUUM button Airis removed from the rotor chamber You can skip this step o Temperature control starts o The indicator of the VACUUM button displays the vacuum fame level in the rotor chamber 1 Low vacuum 1 segment 2 Medium vacuum 3 Hig vacuum 3 segments o If there is moisture or frost in the rotor chamber it will take a long time to reach a medium or high vacuum level Use a cloth or similar material to wipe away any moisture or frost o Seta high vacuum level before pressing the START button to process samples sensitive to temperature rises 3 16 3 2 5 Operation Procedures Touchscreen operation Centrifuge operations and notes Press the START button Operation stops when the set centrifugation time has elapsed end of run Or press the STOP button Oo The rotor stops Press the VACUUM button The START button lamp blinks and the rotor starts turning The timer begins operating If the actual run timer is set the timer will start operating when set speed is reached When the set speed is reached the START button lamp changes to steady lighting and
49. Crown AN CAUTION Clean the inside of the drive hole crown hole of the rotor and the surface of the drive shaft crown of the centrifuge once a month If the drive hole or the drive shaft is stained or any foreign matter is adhered the rotor may be improperly installed and come off during operation This part is very important because the rotor is mounted on it and the crown transmits driving force to the rotor Before mounting a rotor wipe the outer surface of the crown with a soft cloth dampened with water sufficiently Chamber door seal Drive shaft crown Temperature sensor Fig 4 1 Rotor chamber 4 2 3 Cabinet 4 Others KB 4 3 Cabinet Always keep the top deck and the cabinet of the centrifuge clean to prevent dust and other materials from falling into the rotor chamber Wipe the top deck and the cabinet with a cloth or sponge dampened with a diluted solution of neutral detergent If any solution that is toxic radioactive or pathogenic is spilt inside or outside the centrifuge take necessary action according to your proper laboratory procedures and methods 1 Storage period of service parts Service parts are kept in stock ten years after the discontinuation of production The term service parts means the parts that are necessary to ensure the correct functioning of the centrifuge 4 3 5 Troubleshooting 5 Troubles
50. GLORESEEG he Defrost setting in the MENU screen wR MES Mh screen appears DEFROST button 2 If the rotor is rotating press Ser ee Ly et The DEFROST DEFROST SETTING the DEFROST button If the E39 button is surrounded by a yellow frame to rotor is standing still check ndcatethat that the door is closed before defrosting has pressing the DEFROST started button To stop defrosting press the Normal button o Pressing the DEFROST button when the rotor is standing still will immediately start defrosting If the DEFROST button is pressed when the rotor is standing still and the door is open the function will be disabled a few seconds later and the Function Selection Area in the Run screen will display a Door open alert Be sure to close the door when starting defrosting after the rotor stopped 3 Press the Run screen tab o The touchscreen displays the Run screen again o Defrost is indicated above the VACUUM button Defrost 3 70 3 4 4 Defrost Reference As described below the defrost function operates differently depending on centrifuge status when defrosting is started Evacuation is performed simultaneously with defrosting DN Enabling defrosting when the rotor is Enabling defrosting when the rotor is standing still rotating Defrosting starts when selected and Defrosting starts when rotor speed starts continues for 10 minutes When to decelerate and continues for 10 defrostin
51. If this happens balance the samples more accurately a Within 5 mm T Within 5 mm 2 Bottles Fig 3 1 2 Balancing samples Note that partially filled tubes may impose speed limitations or lead to tube failure with some rotors Tubes or bottles must be full in the following cases 1 When thin tubes or seal tubes are used 2 When thick tubes are loaded in a swing rotor 3 When a bottle is used 100 000 x g or more For more information on rotor and tube handling read through the Rotor tube bottle and cap instruction manual and the instruction manual supplied with the rotor 3 2 Basic Operation 3 2 1 Setting Run Parameters 3 2 Basic Operation A WARNING 1 Do not incline or move the instrument while the rotor is spinning Do not place any object on the instrument or lean on the instrument 2 The centrifuge itself may move if the rotor fails during high speed rotation Make sure there is a 30 cm area around the centrifuge that will allow for such movement and do not allow individuals to enter into that area during operation Also do not place dangerous objects such as flammable or explosive materials on top of the centrifuge or in the surrounding area A CAUTION 1 Do not press the touchscreen with a sharp pointed object such as a ballpoint pen 2 If abnormal sound or vibration occurs stop the operation immediately and contact a Thermo Fisher Scientific authorized sales service representative 3 2 1 Setting
52. M button indicator lights 2 High button When the vacuum level in the rotor chamber Fig 3 6 6 6 Start vacuum reaches about 13 Pa the vacuum waiting time Setting screen ends and the rotor starts to accelerate to reach the set speed This occurs when three segments in the VACUUM button indicator lights Press to select either the Medium or the High button Confirm that the selected button is surrounded by a red frame before pressing the X button or ADMIN tab to store the setting 7 Zonal speed Zonal speed can be set in increments of 100 rom between 2 000 and 3 000 rpm pee ZONAL SPEED Touchscreen operation Screen displays and notes Press the ZONAL SPEED UN Sereen Tere eee The ZONAL speed button IANCU REGEL setting screen 300 appears and the 2 000 3 000rpm currently set speed is et N l Speed displayed in black fm oh Gis characters ZONAL SPEED the on screen keypad and Pressing the Enter button turns the ZONAL speed 2 Enter the desired speed on The entered value is displayed in blue press the Enter button indication black showing that the setting has been recorded button the Run screen tab or ADMIN screen the MENU tab Press the Run screen tab or MENU tab to return to the respective screen Press the ADMIN tab the X Press the ADMIN tab or the X button to return to the 3 99 3 4 6 Admin Function 8 LAN communications Connects and disconnects LAN
53. Pressing this field will change the display in the following order SPEED gt RCFmax gt nn In the history of w T operation the run time field is displayed in gray Pressing this field will change the display in the following order TIME gt wT o When an alert occurs the Stop condition field provides details on the alert 3 60 3 4 1 Outputting and Reusing Run History Display and Run Parameters Touchscreen operation Screen displays and notes Press the Load for a new o The touchscreen displays the Run screen again and the run parameters in the history are set run to use the run parameters in the Run history details screen Press the X button to view a The touchscreen displays the Run history screen again different run history If the run history is no longer The touchscreen displays the Run screen again needed press the Run screen tab NOTE In the Run History screen dates with red underlines indicate when an alert occurred and the Stop condition field in the Run history details screen provides information on the alert 2 Using Run History output and User rotor run history 2 1 Outputting Run History Touchscreen operation screen displays and notes Press the Run History button Eme RETER The Menu box in Run in the MENU screen and press an pat BREl History appears the Menu button at the bottom left in the Run History screen MENU box 5 10 Oct 13 10 40 41000 TH 641 Page 00
54. Run Parameters This section will describe the Run screen the starting point of basic operations For information on screens displayed during normal operation and entry of run parameters refer to Section 2 2 1 Touchscreen and External Connections Normal operation display The screen for displaying run parameters and operating conditions is called the run screen SPEED TIME and TEMP are displayed in two rows the top row displays the current operating condition while the bottom row displays the setting value The acceleration ACCEL and deceleration DECEL fields display setting values 24 Oct 2013 9 30 Operating condition display field Setting value display field Acceleration j ae deceleration display fields ACCEL DECEL ACCEL 9 DECEL 9 K 3 5 3 2 1 Setting Run Parameters Display and operations when entering run parameters Press the SPEED TIME TEMP ACCEL or DECEL field on the touchscreen to display the on screen keypad 1 Press the field of the item you want to set The initial digits are displayed in blue SET On screen keypad 2 Press the on screen keypad to enter a numeric value Example 100 000 rpm Press 1 0 0 0 SET 100 000 3 If you do not want to make any other settings press the Enter button on the on screen keypad If you also want to make other settings press the field you want to set This completes the first SET 100000 _ Setting
55. X button to return to the ADMIN button the Run screen tab or sala the MENU tab o Press the Run screen tab or MENU tab to return to the l respective screen In the factory defaults the user name Admin is registered as having an access level of Administrator and a PIN of 1111 3 87 3 4 6 Admin Function 2 Method for deleting users and changing registered information Touchscreen operation screen displays and notes Press the User eminen oem The Admin screen Management button ghee apenan E replaces the user User name Access level Last run 7 Administrator 23 Oct 2019 15 45 management screen w Af 02 Oct 2013 13 20 P Johnson Administrator 15 Oct 2013 3 15 User Management de o o i Sree 16 Jun 2013 14 00 Administrator Press the User field of the ME ri AEM The selected user User Management i user you want to delete or User name Access level Last run ih elie from whose registered information Administrator 23 Oct 2013 15 45 white to diue you want to change 02 det 2013 13 20 Selected user field Press the MENU button m it Eu The Menu box appears MENU Administrata Press the Delete user PUN Scree E toe A delete confirmation User Management messade a ears button to delete a registered terre ENTEN Ea ge app Administrator 23 Oct 2013 15 45 02 Oct 2013 13 20 15 Ost 2013 9 15 Delete confirmation Are you sure you want to me
56. a You can control total run time and number of runs _ For details refer to Section 3 4 6 3 otor Management You can restrict the number of users For details refer to Section 3 4 6 2 Run time indication Lapse Remain Allows you to change the centrifuge run time indication For details refer to Section 3 4 6 4 Lapse Remain Actual run timer For details refer to Section 3 4 6 5 nena Run Imer Allows you to select the actual run timer 3 84 3 4 6 Admin Function Name Icon Function Allows you to set the vacuum conditions when the centrifuge starts accelerating from vacuum standby Vacuum level state to the set speed For details refer to Section 3 4 6 6 Zonal speed can be changed in increments of 100 rom Zonal speed ee between 2 000 and 3 000 rpm For details refer to Section 3 4 6 7 LAN communications 1 Connects and disconnects LAN communications For details refer to Section 3 4 6 8 LAN Communication Press the icon for the function you want to use and select from the options that appear After completing the settings press the tab of the screen you want to return to for example the Run screen 1 User management dap v The centrifuge can register store up to 50 users that are managed at three Af different management levels Methods for registering users and changing user information are described below User User registration makes it possible to
57. a white dot of light starts to spin around the button The rotor stays in standby at 4 000 rpm until a medium vacuum level is reached The STOP button lamp blinks and the rotor starts decelerating The STOP button changes to steady lighting The buzzer sounds to indicate the rotor has stopped The vacuum pump stops the air leak valve starts operating to return the rotor chamber to normal atmospheric pressure The door lock is released and the door can be opened When the rotor chamber returns to normal atmospheric pressure the Now open the door message appears above the VACUUM button Now open the door Remove the rotor o Gently remove the rotor when it has stopped If the rotor chamber is not properly evacuated before operation or room temperature is low 10C or less the evacuation may take time and the centrifuge will operate at 4 000 rpm If the chamber is not properly evacuated the rotor may slow down before the set speed is obtained to continue evacuation Therefore it is recommended that you use the VACUUM button before operation to evacuate the chamber for about 15 minutes or until the indicator above the VACUUM button indicates that a high vacuum level has been reached 3 2 5 Operation Procedures Fig 3 2 3 is an overview of the procedures described above see Section 3 4 3 Zonal Operation Procedures for information on using a zonal rotor Start of operation Turn on the Dep centrifuge POWER
58. acentrifuge 2 Description 2 1 External View of Ultracentrifuge The Sorvall WX Ultra series centrifuges are floor standing types These three types of ultracentrifuges have the same external view except for the model name printed on front cover and the same dimensions The following is the external view of the Sorvall WX100 Ultracentrifuge Unit mm LED Indicator POWER Switch External Connection Touchscreen display operation NOTE This height is measured from level floor surface Fig 2 1 External view of Sorvall WX100 Ultracentrifuge 2 1 2 2 Structure 2 2 1 Touchscreen and External Connection 2 2 Structure 2 2 1 Touchscreen and External Connection The touchscreen with color liquid crystal screen is incorporated in the Sorvall WX Ultra series centrifuges You can set the run parameters perform the operation and display a run history programmed operation and user customizations screens by pressing the screen Fig 2 2 1 shows the touchscreen Tabs of each screen 5 Function 1 Display Areas Selection Area ACCEL DECEL ACCEL 9 DECEL 9 KR 2 VACUUM 4 STOP button button Indicator 3 START button Display at the normal operation The following screen appears by pressing the SPEED TIME or TEMP button 24 Oct 2013 9 30 TIME hh mm 6 On screen keypad Display when setting the run conditions such as the speed etc Fig 2 2 1 Touchsc
59. ake centrifugation time into account and centrifugation start time should be set to a time later than current time Press the Enter button meri Maree A message prompting you to check operation appears Press START button Press STOP button to cancel T 8100 RTC ACCEL J pr 4 Press the START button TTEA 2013 16 30 RTC will not start unless a d RTC disola you press the START T 8100 erma SOET Eed play button aceea tP aabot 30 Upper row RESEE AS Operation start time Tal Lower row The STOP button allows k Operation stop time you to cancel the RTC operation setting o The vacuum pump goes on and temperature control starts o The RTC display screen shows the START time and STOP time indicating that RTC operation is ready to start o The centrifuge will start automatically at the set time 3 56 3 3 4 RTC Real Time Control Operation NOTE 1 In the following situations RTC cannot be set 1 When TIME run time is set to HOLD continuous operation in the Run screen C 3 Change the run time centrifugation time to a numeric value instead of HOLD 2 When it is past the start time L7 Set the start time to a time later than the current time 3 When the start time is more than 20 days later than the current date SE Set a time to a time that is within 20 days of the current date 2 To change the TIME run time in the Run screen after setting RTC cancel RTC and reset the TIME To ca
60. am Registration Procedures 1 Procedures for registration of run parameters Procedures for registering run parameters are described below Touchscreen operation Screen displays and notes 1 Turn on the centrifuge PPro The Run screen POWER switch PAE l P appears SET ROTOR ACCEL DE CEL ACCEL 9 DECEL 3 Press the Program button in the Function Selection Area changes to the Area in the Run screen Program display screen 7 F i s Program display screen ACCEL DECEL S ___ ACCEL 9 DECEL 9 fel o AProgram No displayed in the Folder button indicates that the program run parameters have been set in that program Folder button 3 23 3 3 1 1 Program Registration Procedures Touchscreen operation screen displays and notes 3 Press the Folder button in PROGRAM The Run screen the program display screen 0001 0002 0003 gt replaces the Pett pai o vohmec ee eee Program list screen gt noona PN a Program folders 3 Le a jota 00s 0006 me me EN come in four colors VLDL i H Lung E oa o mia The program list can 0007 ay be displayed in aoe ai PETI different colors en i l New Folder button Program list screen L All folder button Press to display all set programs on the screen in number order PST Blue folder button Press to display only programs set in blue folders on the screen PE Red folder button Pres
61. an store two or more run parameters in one program memory area and a total of 30 steps This allows it to change speed run time temperature and other conditions during operation For example you could set the speed of a step to 0 rpm to stop rotation to enable opening and closing vacuum leaks and doors This makes it possible to register in advance the repeat count of samples that are removed and processed each run under the same separation conditions which prevents processing samples too many or too few times Operation procedures are described using examples below 1 Registration Procedures for Step Mode Operation Setting example The following describes the setup procedures for continuous operation under the three step run parameters shown in the figure below In this example the operations described in Fig 3 3 1 1 will be performed automatically S Step 1 Step 2 Step 3 100 000 rom 80 000 rom 60 000 rom Acceleration mode mode 100 000 Speed 80 000 Pm 60 000 1h 30 min 1h 30 min Fig 3 3 1 1 Example of Step Mode Operations 3 41 3 3 1 3 Step Mode Operation Procedures Touchscreen operation screen displays and notes As described in steps 1 to 8 Program registration screen Run parameter setting display in 1 Procedures for a a registration of run Repor ee ien parameters in Section 3 3 1 1 Program User pane registration procedures enter the Program No registered rotor
62. atch en Na cata Gea Rath aka aE nat Meat Aor ahah dees 5 4 5 3 User Corrected Problem er ee ey eo Te ae re a en See en E Ran a en ee See er ae gern een ee ae eae 5 4 6 Installation sper nant puto incon hunt stats yds A PAASO stati dort AAA SETE MI AAEE SO EA erty Mita EEE EEN EAE 6 1 7 Warranty for the WX Plus Ultra Series Centrifuge re 7 1 8 Supply List Beat elec rte hap ees adv a cea tata a Ze ehcp sce astm iit wheat Sak ive ag de th ae tee tot tar E A peat eta saunas atc 8 1 1 Specifications 1 Specifications Sorvall WX100 Plus Sorvall WX90 Plus Sorvall WX80 Plus 100 000 rom 90 000 rom 80 000 rpm Maximum RCF 802 000 x g 692 100 xg 602 600 x g T 8100 T 890 T 880 Speed control accuracy 10 rpm 1 000 rom to maximum speed Acceleration deceleration 19 stage variable acceleration control 10 stage braked deceleration control control plus coasting deceleration Rotor temperature o p 5 control display accuracy 0 5 C set temperature is ffrom O C to 40 C Set speed 1 000 rpm to maximum speed in increments of 100 rom Set ti One minute to 999 hours and 59 minutes in increments of one minute SEARS Hold for continuous operation Oil rotary vacuum pump and oil diffusion pump combined Vacuum system Ultimate vacuum below 1 3 Pa 0 01 Torr This value reaches within 15 minutes after the rotor starts rotating measured 1m in front of the instrument AARU Heat 1 kW or less dissipation into room Cooling met
63. atement verifying decontamination what was the contaminant and what decontamination method was used If the centrifuge must be returned to a Thermo facility 1 Contact your Thermo representative to obtain a Return Service Order Number RSO No be prepared with the name and serial number of the centrifuge or rotor and the repairs required Send item s with the RSO No clearly marked on the outside of packaging to the address obtained from your Thermo representative NOTE United States federal regulations require that parts and instruments must be decontaminated before being transported Outside the United States check local regulations If the centrifuge to be serviced does not have a Decontamination Information Certificate attached and in Thermo s opinion presents a potential radioactive or biological hazard the Thermo representative will not service the equipment until proper decontamination and certification is complete If Thermo receives a centrifuge at its Service facilities which in its opinion is a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Disposition costs will be borne by the sender Decontamination Information Certificates are included with these instructions Additional certificates are available from the local Account Representative or Field Service Engineer In the event these certificates are not available a written statement certifyin
64. ates to the set speed and stabilizes 1 Set run time starts counting down when you press the START button However you can change the run time range to count down only when the rotor turns at a stable speed Refer to Section 3 4 6 5 Actual Run Time 2 Run time during vacuum standby 4 000 rpm is not counted down 3 68 3 4 3 Zonal Operation Procedures Step Touchscreen operation Instrument operations and notes The set time elapsed Time out 11 12 1 14 To stop before the set time elapses press the STOP button When the rotor stabilizes at zonal speed press the VACUUM button Open the door remove the cap install the seal assembly and unload the samples Press the STOP button To stop zonal operation press the MENU tab and press the ZONAL setting button in the MENU screen Press the NORMAL button check that the button is now surrounded by a yellow frame before pressing the Run screen tab When the set time elapses the rotor decelerates and stabilizes at the zonal speed The zonal operation chart uses a steady or blinking red light to indicate the progress of the run When the speed stabilizes a buzzer sounds The vacuum pump stops and the rotor chamber is vented to return to normal atmospheric pressure The door lock is released N CAUTION The cap is removed by hand from the rotor and the seal assembly is installed when the rotor is turning Perform
65. bleshooting easier and quicker The current centrifuge status is identified at a glance by the signal light of the upper front edge of this centrifuge 10 Space saving design The installation area required is 0 72 m 800 x 900 mm Lower top deck makes it easy to install and remove the rotor 11 These products spin very quietly and are thus well suited for personal use 12 Samples can be easily balanced visually 13 A CFC free thermomodule cooling system is employed featuring a powerful cooling capacity 14 In addition to door lock and an imbalance detector dual overspeed detection system is incorporated for overspeed detection for even greater safety Note1 RCF Relative Centrifugal Force N SAFETY NOTICES N Safety reminders Carefully read and fully understand the following safety instructions e Operate your instrument according to the instruction manual e Be sure to observe the all safety precautions in the instruction manual and safety instructions on your instrument If neglected personal injury and or instrument damage can be caused e f the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired e The safety reminders are indicated as shown below The signal words DANGER WARNING and CAUTION are indicated together with the hazard alert symbols in this manual AN DANGER This note indicates an imminently hazardous situation w
66. cece 3 52 3 3 4 RTC Real Time Control Operation N Dyn dee ssa Th ee E locate a E ae len pee ahaa 3 54 3 4 Features of the MENU Screen ee ee ee ee ee ee ee ee ee 3 58 3 4 1 Outputting and Reusing Run History and Run Parameters iecere eneee 3 60 3 4 2 Rotor Catalog Spbuatwni urate Qe ati leek etl a Oak aS ct ha da ol doe lia r a aaa 3 64 3 4 3 Zonal Operation Procedures circ ttt et et eet e nee eeeeeeeeeeeeeeenenenenes 3 66 3 4 4 Defrost ccc eee ence ence eee eee e nee ee en een ee eeeeeeeeneeeeneeeenenes 3 70 3 4 5 Customizing AE A TAA A Wa ruta EA T E E E E A 8 oot 3 72 1 Zoom display setting E a a a eee pea pratt Abt Read ew ie A ae na 3 73 2 Stop signal setting esas EN EA a T Meat an tod T EA Sa EE Wile sare ase E a a EA aE Ede 3 73 3 Sound volume adjustment Gah ese eel a5 ps E a E E E 9 E E T 3 74 4 Backlight adjustment and dimming backlight mode setting 070eeeeeeeees 3 74 5 Language Switch button crete eee eee e ee eeennenenes 3 75 6 Schedule cccctc ccc tenes eeeeeeeeeeeeeeeeeeeeneeeeneneeeeeenee 3 75 7 Date time setting a aap early a a e a a atch eon cca E caw ee oo wade 3 78 8 Status display LED Indicator lamp setting Seaplane WE Rin Re RE EE Ra SR 3 79 9 Economy mode setting Oe Nee Ce TOR aT a E AME a ne MER ee Pe Re ee 3 89 Table of Contents CeIn ete ai apl Eune on oa a eee ee ere A ere er ares errr rrr 3 84 1 User management 1 1rrttett treet etter eee eee rere tenner 3 85 2 User lockout i ihr a i tne he eh SY a
67. cle Hold the plug when disconnecting it 6 3 6 Installation 5 Leveling 1 Turn the four leveling screws with a wrench to lift the caster about 10 to 20 mm off the floor as shown in Fig 6 3 2 Turn on the POWER switch and open the chamber door Then turn off the switch again If the power cord is not yet connected remove the front cover and then open the door according to the instructions given in Section 3 5 Happenings when power failure occurs 10 20 mm Leveling screw Fig 6 3 Leveling operation 3 Place the level across the top of the drive shaft in the rotor chamber see Fig 6 4 Turn the four leveling screws until the bubble in the level indicates the instrument is level 4 When the instrument is level check that the four leveling screws are secured to the floor Fig 6 4 Level Placement 5 Moving the ultracentrifuge When moving the ultracentrifuge disconnect the power cord and unscrew the leveling screws with a wrench to lower the caster to the floor Raise the leveling screws enough to remove the leveling pads then move the ultracentrifuge After moving be sure to install and level the instrument again CAUTION Be sure to remove the rotor from the rotor chamber when centrifuge is moved After installation and before any test run this ultracentrifuge always needs the internal check by a Thermo Fisher Scientific authorized sales service representative 6 4 7 Warranty for the WX Plus Ultra Se
68. communications When the centrifuge is connected to a LAN the LogManager software can be used for communications and storing operation results PAN To connect to a LAN insert a LAN cable into the LAN port in the external Communication Connections on the right side of the instrument 1 Connect button Starts LAN communications When the centrifuge is connected to a LAN an icon indicating LAN connection status appears on the right side of the Menu tab as X The LAN is correctly connected Connect Disconnect 24 Oct 2013 48 30 LAN Coamuni cation x The LAN is not properly connected Check LAN cable connection on the instrument Fig 3 4 6 8 Set LAN communication side start up the LogManager screen and check LAN network operation 2 Disconnect button Stops LAN communications Press to select either the Connect or the Disconnect button Confirm that the selected button is surrounded by a red frame before pressing the ADMIN tab or X button to store the setting 1 Some screens may not display a LAN connection status icon 2 The LAN port is located among the external connections on the right side of the instrument refer to Section 2 1 External view LAN port External connections 3 100 3 4 7 Instrument ID Service Contact Information 3 4 7 Instrument ID Service Contact Information _ When multiple instruments are used set separate IDs in each Service contact Hi information and
69. cons are described below Function The centrifuge can automatically store up to 5 120 run parameters for past runs For details refer to Section 3 4 1 Outputting and Reusing Run History Display and Run Parameters Allows you to browse available rotors and their specifications For details refer to Section 3 4 2 Rotor Catalog NORMAL Zonal Settings SY Allows you to switch between normal and zonal operation For ATA details refer to Section 3 4 3 Zonal Operation Procedures Setting 3 58 3 4 Features of the MENU Screen Name Icon Function Starts the Defrost function that heats the rotor chamber to quickly remove frost and moisture For details refer to Section 3 4 4 DEFROST Defrost Defrost DEF function Customize settings in a way that works best for you For details refer to Section X Allows you to adjust screen displays the stop signal and other 3 4 5 Customizing Customize Manager Allows you to set administrator functions such as user lockout etc Admin For details refer to Section 3 4 6 Admin Function Manager Admin Instrument ID mem Enter an ID to identify your instrument Service For details refer to Section 3 4 7 Instrument ID Service Contact Contact ID Contact nformation Press the icon for the function you want to use and select from the options that appear 3 59 3 4 1 Outputting and Reusing Run History Display and Run Parameters 3 4 1 O
70. creen appears in the run screen Arun parameter called 0000001 ACCEL DECEL ACCEL 9 4 Program display screen T 8100 from the program DECEL 7 Fai Remaining run time for Arun parameter called aN Seps from the program The number of the called program is displayed in the Program display screen It allows you to set and display run parameters SPEED TIME TEMP ACEL DECEL ROTOR USER The run time for the 0 rpm setting is not included in the remaining run time for step operation The vacuum pump starts operating and temperature control starts The START button lamp flashes and the rotor starts turning The indicator of the VACUUM button displays the vacuum level in the rotor chamber The time starts counting When the set speed is reached the START button lamp changes to steady lighting and a white dot of light starts to spin around the button The rotor stays in standby at 4 000 rpm until a medium vacuum level is reached 3 47 3 3 1 3 Step Mode Operation Procedures Touchscreen operation Screen displays and notes When run time for step 1 has TN Weare The Program display elapsed processing proceeds ieee tiers to step 2 and the rotor sens decelerates and stops Arun parameter called faccet o H Di Romi ol ia from the program DECEL ra Program display screen o The Program display screen proceeds to step 2 and SPEED is set to 0 rom and TIME to Hold When
71. creens change PROGRAM Wo 0008 The Program Rotor registration screen 1 Rotor model haere display registration field 2 Registered user display registration field 3 Remarks display registration field 4 Folder color display registration field Next button USB EXPORT button For information on USB EXPORT see Section 3 4 1 Outputting and Reusing Run History Display and run parameters Mod The Rotor Management screen appears Rotor Management Name Krpm Runs Undefine Select the rotor you will use and press the OK button If the rotor you want to use cannot be found in the Rotor Management screen press the Rotor Catalog button and select a rotor from the Rotor catalog screen The selected rotor is displayed in the rotor model display registration field 1 in the program registration screen For details refer to Section 3 2 3 Setting Run Parameters 3 25 3 3 1 1 Program Registration Procedures Touchscreen operation screen displays and notes Press the white area in the er Sees The User Management User Management Registered user Access level Last run Eg screen appears display registration field 2 WE aE EAEE to select a user IT l 02 Oct 2013 13 20 Administrator Oct 2013 9 15 super User 18 Jun 2013 14 00 You can omit this operation o Select the user name you want to use and press the LOGIN button o Enter the PIN and press the Enter button
72. d of the Run screen tab l IME hh IP appear in the setting speed according to the value display field in instructions in Section 3 2 1 the Run screen Setting Run Parameters ES 4 0 Setting value display T 4100 000000 i ACCEL DECEL ACCEL a DECEL 3 USER Admin 3 67 3 4 3 Zonal Operation Procedures Step Touchscreen operation Instrument operations and notes Press the Rotor indicator field Nenu Ae The selected rotor to select a zonal rotor as type ARP a i the Rotor indicator field described in Section 3 2 3 Rotor Selection Rotor indicator field Press the START button o The rotor accelerates to Zonal speed normally 3 000 rpm and stabilizes o The zonal operation chart uses a blue light to indicate the progress of the run o Load the samples and install the cap on the rotor bearing CAUTION The cap is installed by hand on the rotor when it is turning Perform this operation with care and be sure to follow the instructions in the instruction manual Press the VACUUM button eee A dialog box to start the vacuum pump and IE UEA appears asking you press the START button to confirm whether it is all right to again ser aH sh accelerate to high Do you want to accelerate to set speed speed Z 32 oodo001 ACCEL DECEL ACCEL 9 DECEL 9 X USER Admin o Check the run parameters and press the YES button o The rotor acceler
73. d re to the right above the display field Fig 3 4 6 4 Set run time indication screen Press to select either the Elapsed or the Remaining Confirm that the selected button is surrounded by a red frame before pressing the X button or ADMIN tab to store the setting 5 Actual run timer You can set the run time to start counting down either when the START button is pressed or when the set speed is obtained Actual Run Timer 1 Enable button Run time is counted down when the set LV ern aks speed IS obtained Actual Run Timer Setting 2 Disable button Run time is counted down right after the START button is pressed Press to select either the Enable or the Disable Confirm that the selected button is surrounded by a red frame before pressing the X button or ADMIN tab to store the setting Fig 3 4 6 5 Actual run timer setting screen 3 98 3 4 6 Admin Function 6 Vacuum level ia Vacuum agi Vacuum You can select a vacuum level when acceleration starts after exceeding the vacuum waiting time at 4 000 rpm Select Start high vacuum to prevent a temperature rise caused by wind Level JUN screen Men 34 Oct 2013 9 30 Select vacuum level at 4000 rpm x 1 Medium button When the vacuum level in the rotor chamber Tm reaches about 133 Pa the vacuum waiting time PT ends and the rotor starts to accelerate to reach the set speed This occurs when two segments in the VACUU
74. d with the rotor pins before operation Wrong setting can cause severe damage to the instrument Make sure that all the rotor buckets are of the same type e f abnormal sound or vibration occurs stop the operation immediately and contact a Thermo Fisher Scientific authorized sales service representative N CAUTION e Before using a rotor be sure to read through the rotor instruction manual e Check the chemical resistance chart attached to the rotor and do not use any sample inapplicable to the tubes tube caps bottles or bottle caps etc Using such a sample could corrode or deteriorate such parts and the sample leakage might occur eUse the rotor tubes and bottles within their actual capacities see the instruction manual e Do not use tubes bottles that have exceeded their life expectancy Failure to do so could result in damage of tubes bottles and the rotor and the centrifuge The life expectancy of tubes bottles depends on factors such as the characteristics of samples speed of the rotor used and temperature Always check for deterioration and damage cracks deformation and so on on tubes bottles before using them Do not use the tubes bottles if you find such a problem e Mount the rotor onto the drive shaft gently and properly Do not drop the rotor or apply excessive force to the drive shaft to avoid damage to the drive shaft J N SAFETY NOTICES N CAUTION Install the rotor carefully and securely on the driv
75. e derates its maximum permitted speed and indicates an asterisk to the right of the krpm indication in the Rotor management screen refer to section 3 2 3 Rotor Selection simultaneous with the change in maximum permitted speed Once the maximum permitted speed is derated the actual speed of the rotor is automatically restricted within the new limits The rotor life management data stored in the ultracentrifuge is not covered by the warranty When a rotor is used with the ultracentrifuge be sure to record the rotor management data in the rotor log book If the rotor log book is not maintained correctly the rotor will not be warranted 3 Automatic rotor exclusion When a registered rotor reaches the end of its life the ultracentrifuge indicates it by displaying a message on the screen Subsequent runs of this registered rotor are precluded 3 93 3 4 6 Admin Function 4 Registering a rotor A standard rotor can be registered when you use the rotor management feature of the ultracentrifuge If the rotor has not been registered the feature does not operate for the rotor To register a rotor use the procedure described below lt Procedure for registering a new rotor Touchscreen operation screen displays and notes Press the User MAEEREE The Rotor Rotor Management Management screen Management button Name insane egies Likes x DE ER Rotor Management Add rotor button Press the Add rotor button
76. e ee ee ee ee ee ee 2 2 2 2 1 Touchscreen and External Connection 1 cc tetas 2 2 9 2 2 Rotor Chambers ccc ccc eens eeeeseeeeeeeeeeeeeneeeeneeeneseenenes 2 6 2 2 3 Safety DEVICES ttttsrsrrrrsrrsrrrrrrrrrrrrrrrrrrrrrrnrrrnrerrnnrnsnrrnnnrrnrrerennensnrrnennnt 2 7 9 2 4 Rotor overspeed fe 0y CE eee eee eee errr rere 2 8 3 Operation ee eee ee eee ee ee ee ea ee rr ee eee rn T 3 4 3 1 Run Preparation ee ee ee ee ee ee 3 3 3 4 1 Starting up the Centrifuge OC ge PRES A eg te neo a ga EER nO Pa eer Seon ae 3 3 3 1 2 Rotor and Tubes Preparations and Precautions s 1 rtrtrtrrttret etter etree 3 4 3 2 Basic Operation ee ee 3 5 3 9 4 Setting Run Parameters tcc cc ene neneeeeeeeeeeeeeeeenerennenenes 3 5 3 9 2 Acceleration and Deceleration Modes i tt tt antennas 3 10 3 9 3 Rotor Selection cc ccc ccc eee eae een ee eeneeeeneneeeneeeeeeneeanes 3 414 SDA Logging masa ljep iink A NE E N a OA A 3 14 3 9 5 Operation Procedures crt eee ene eee e ee eeeneeeneeeeneneeeeneeeenenes 3 16 3 3 How to Use the Function Selection ALC tne enn enna 3 19 3 3 4 Programmed Operation Loi aa aude ie ela oe Saha ah we ane oie teas a eee eaten at ean 3 20 3 3 4 4 Program Registration Procedures crt t net ee nen ee ene nes 3 93 3 3 1 2 Programmed Operation Procedures tttirssrstsrtrrsrsrrsrrrrsrrrrnrrnnerennnas 3 37 3 3 1 3 Step Mode Operation Procedures i tenet eee enee nena 3 44 3 3 2 RCF Relative Centrifugal Force Display and Setting Function verteten 3 49 3 3 3 wT Operat on
77. e mechanical resistance of the drive motor 2 DECEL code F A deceleration method that does not involve brake control using the drive motor The deceleration time will then vary widely depending on differences in the small mechanical resistance of the drive motor and the vacuum level of the rotor chamber and other factors Examples showing use of acceleration and deceleration modes Optimum numeric code Separation characteristics Density gradient As the density gradient reorients during centrifugation using a acceleration and deceleration the gradient may be vertical rotor 5 disturbed if samples are rapidly accelerated and decelerated Using CsCl You can operate at maximum acceleration because density gradient the density gradient is not formed during the run sedimentation equilibrium Slow deceleration is recommended to obtain sharp centrifugation for DNA bands separation when using a uniform liquid Pelleting using an angle Rapid unloading of samples is possible rotor short separation time Density gradient The sample and gradient do not reorient For that centrifugation using swing reason there is less gradient turbulence than with a bucket rotor vertical rotor and good results can be obtained as long as rapid acceleration or deceleration are avoided With a swinging bucket rotor there is little difference with regard to turbulence if ACCEL DECEL is less than or equal to 8 However rotor swinging may become large in
78. e shaft crown in the rotor chamber Always place the rotor pin in the drive hole crown hole apart from the crown pin eTake care not to get your hands or fingers caught in the door of the centrifuge e Maximum rotor speed depends on the tubes or adapters to be used Follow the instructions on the rotor instruction manual e Approximately even quantities of sample in the tubes are sufficient for balancing and extremely different sample quantities must be avoided e Clean the inside of the drive hole crown hole of the rotor and the surface of the drive shaft crown of the centrifuge once a month e Storing the rotor on the shelf is permitted if the shelf is taken necessary countermeasures against earthquakes not to drop the rotor e Do not pour any solution such as water detergent or disinfectant directly into the rotor chamber Be careful not to leak the sample Failure to do so might cause the bearings of the drive unit and or the sensors to corrode or deteriorate e Use the rotor log book to manage the life of the rotor e t is important to manage the life of the rotor The life of each rotor is specific and dependent upon the frequency and the total running time Do not use rotors whose lives have expired If used the machine can be seriously damaged Follow the rotor instruction manual e For details of the Zonal centrifugation see the Zonal Rotor instruction manual e Do not press the touchscreen with a sharp pointed object
79. ection pet Se ee ad Rater ie ie to oO ial registration procedures 1 hour Program registration Run parameter setting display screen PROGHAM Ao O01 PHGEH AM Aio ca Admin enter the Program No i sar 3 i i U a 1c s 1 ke m es Ez z registered rotor user and TERN remarks select a folder color ibasome ness T and press the Next button mgr aa g In this example the steps are stored in program No Next button Edit button 11 blue folder 3 45 3 3 1 3 Step Mode Operation Procedures Touchscreen operation Screen displays and notes 2 Enter the run parameters for it E x The run parameter Si area aa rea sl ci ala Registration Procedures for appears hoe sabato 3 4 a ae STEP 1 SPEED 50 000 rpm TIME 1 hour TEMP 15 0 C ACCEL 9 DECEL 7 o Registering a rotor will make it possible to compute and display RCFmax and RCFavg values from the set SPEED value Press the Next Step button Finer The run parameter and enter the run entry display screen parameters for step 2 from in step 2 appears the run parameter entry display screen STEP No STEP 2 Next Step button SPEED 0 rpm TEMP 15 0 C dan o When a SPEED of 0 rpm is set TIME is automatically set to HOLD o An arbitrary TIME setting cannot be made Press the Next Step button and enter the run parameters for step 5 from step 3 5 Finally press the Re
80. ely that you chose are not applicable Check whether ACCEL DECEL code Applicable ACCEL DECEL code number setting is applicable to this numbers are limited depending on rotor see the rotor instruction manual the rotors the rotor 5 1 Alert Indicators O me O c O y o O SET ROTOR or 1 Incorrect rotor is selected s Gajec the corect rotor 2 No rotor is installed NO ROTOR e Install the rotor e Lower the room temperature eRemove the objects from the surrounding of the centrifuge 1 High room temperature ROOM TEMP 2 The surrounding of the centrifuge does not have a free circulation air 1 Required level of vacuum cannot be reached 2 After a satisfactorily high level of VACUUM vacuum was reached it lowered due to for instance sample leakage 1 The VACUUM or START button CLOSE DOOR has been pressed with the chamber door left open Power supply 1 The power supply voltage lowered e Check the power supply voltage voltage e Check that the total number of runs e Wipe off the moisture from inside the rotor chamber e Clean the chamber door seal then apply a thin coat of vacuum grease Refer to Section 4 1 e Check if the sample is leaking from the rotor and or tubes e Close the door completely and press the VACUUM or START button 1 The rotor life almost reaches the final life Secondary life When a Rotor life 1 alert signal Rotor Life 1 occurs i
81. ent The power source specifications are indicated on the rating label which is on the left side of the instrument Be sure to read the rating label If the power source specifications do not match the available power source you can change the power source specifications by manually selecting the desired tapping on the internal transformer Provide an emergency switch circuit breaker intended for this centrifuge only to turn off the centrifuge power in the event of failure It is recommended to provide that switch at the outside of the centrifuge room or near the exit of the centrifuge room Additionally this emergency switch should be marked as the disconnecting device for this centrifuge Your ultracentrifuge can be operated on one of the following four power voltages 208 Vac 50 60 Hz 20 A 220 Vac 50 60 Hz 20 A 230 Vac 50 60 Hz 16 A or 240 Vac 50 60 Hz 16 A You can change the voltage by manually selecting the desired tapping on the internal transformer When the plug Part No 84440401 is included in the accessories of your centrifuge and you connect the power cord to the wall receptacle do not position an object so that it is difficult to disconnect the power cord from the receptacle If you do so you cannot disconnect the power cord from the receptacle when you observe some abnormality 2 Place of installation 1 Install the ultracentrifuge on a concrete stone or hardwood floor Avoid such places as soft floor carpeted
82. ered in step 2 and The PIN is confirmed and the User management screen reappears press the Enter button The user name cannot be changed If such a change should be necessary delete the user name and make a new registration 3 89 3 4 6 Admin Function 2 User lockout User lockout when enabled makes it possible to control user access at three different levels User Lockout 1 Method for setting user lockout Log in as a user as described The user that logs in must have Administrator privileges in Section 3 2 4 Logging in as a User Press the User Lockout un Si Peevey The Set user lockout button User Lockout Setting X screen appears Enable o The selected button is shown inside a red frame in the example Disable is selected 7 A dialog box for User Lockout Setting Press the Disable button enabling user lockout appears at User Lockout is enabled o This dialog box does not appear when lockout is changed from enable to disable The Disable button is surrounded by a red frame and user lockout is disabled 7 Press the YES button o The Enable button is surrounded by a red frame and user Press the Enable button im 24 Oct 2013 9 30 2 3 4 lockout is enabled o Press the ADMIN tab or the X button to return to the ADMIN screen 2 Method for enabling user lockout Press the USER area in the Run screen as described in Section 3 2 4 Logging in as a User
83. ert message appears this alert message is impossible to clear until the rotor stops Be sure to contact a Thermo Fisher Scientific service representative if the alert message VACUUM persists even after you have done what is specified above There might be abnormality in the heater of the oil diffusion pump Be sure to contact a Thermo Fisher Scientific service representative if the alert message E35 DP heater thermistor abnormality appears There might be abnormality in the heater of the oil diffusion pump 5 3 User Corrected Problem If the ultracentrifuge does not function even if no problems are reported do the following The ultracentrifuge The circuit breaker connected e Reset the circuit breaker and turn on the can not be to the ultracentrifuge is POWER switch energized be by tripped turning ON the POWER switch The rotor cannot Poor vacuum e Check whether the oil of the vacuum pump cool down or needs replacing the rotor temperature e Clean or replace the door seal O ring is rising The room temperature is eif there is an air conditioner working near higher than 30 C the ultracentrifuge run it at a lower room temperature setting e f there is no air conditioner working near the ultracentrifuge lower the set speed The rotor surface contains e Wipe water off the rotor with a soft cloth drops of water The window part of the eWipe water off the temperature sensor temperature sensor contains wit
84. es reasonable service and what constitutes a reasonable period of time shall be determined solely by Thermo Fisher Scientific after Thermo Fisher Scientific is in possession of all the facts concerning operating conditions and other pertinent factors and after such component or accessory has been investigated and or factory inspected by Thermo Fisher Scientific 2 All items claimed defective must be returned to Thermo Fisher Scientific transportation charges prepaid and will be returned to Buyer with transportation charges prepaid Thermo Fisher Scientific will be released from all obligations under this warranty in the event that any such instruments have been installed by or repairs or modifications are made by persons other than its own or service personnel authorized by it unless such installation modification and or repairs by others are made with the prior written consent of Thermo Fisher Scientific 3 Thermo Fisher Scientific is not obligated to incorporate into any instrument any design engineering or performance change developed after delivery of the instrument to the original purchaser In addition to the foregoing one 1 year warranty and subject to the foregoing exceptions and conditions Thermo Fisher Scientific warrants the drive assembly of the Sorvall WX Plus Ultra series centrifuge to be free from defects in material or workmanship for ten 10 years from the date of ultracentrifuge installation subject to all the conditions li
85. ess the YES button in o The file selected in the program list is deleted the delete confirmation message A program that is deleted cannot be restored Check carefully before you delete l g lt 1 D laker 0 3 Press the Delete button in imam A delete confirmation the Edit field m me message appears T Sato f Admin i Johnso 3 34 3 3 1 1 Program Registration Procedures 4 Operation procedures for copying programs The following describes how to copy a registered program and register the result as a new program This is a convenient method when you want to register a new program by changing part of a registered program Touchscreen operation screen displays and notes 1 Open the Program list screen following step 1 in 2 Procedures for Changing Program run parameters Press the program folder you IR The Program list want to copy from the 0001 r7 0003 screen appears program list ite Pr ins The example shows how to Pe m si _ Program No copy program No 0008 YEDE H Luna Admin The selected folder is surrounded by a blue frame MENU button 3 Press the MENU button PROGRAM E o The MENU box appears O00 bie in the Program list T Sato Admin fore a lt i screen rs h MENU x s me Pi Bl MENU box VLDL H Lung 7 J 4 a 0007 T ma RNA Admin Ce i m mo ao or a Copy button eh Sita ees Press the Cop
86. etting value is to the instructions in Section h a a a ing V 3 2 1 Setting run parameters een TOR FUNGTION display field T 5100 ws ACCEL DECEL RCF wT 5 ACCEL 8 a RTC button o When setting run parameters do not set HOLD but enter a numeric value for the run time separation time Press the RTC button in the ers Morena The setting value Function Selection Area in m O gt fy A is displayed in the setting value the Run screen display field ACCEL DECEL fea a RTC display Press the RTC display screen Start Time button Stop Time button o The current date and time is displayed the month is displayed in blue and the unit is ready to accept entries 3 55 3 3 4 RTC Real Time Control Operation To set the start time press Men 24 Oct 2013 16 30 the Start Time button and to set the stop time press the Stop Time button Enter the start time and stop time from the on screen keypad Set state display o Pressing the Start Time button will display the Start Time in the set state display and pressing the Stop Time will display the Stop Time in the set state display Pressing the input fields will make them ready to accept entries Use the lt and gt buttons to switch between items month day hour minute Enter a numeric between 0 and 23 24 hour clock for Hour Enter a date and time later than the current time The stop time should t
87. expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use Chapter 4 Do not pour any solution such as water detergent and disinfectant directly into the rotor chamber Otherwise the bearings of the drive unit may be corroded or deteriorated Section 4 1 Clean the inside of the drive hole crown hole of the rotor and the surface of the drive shaft crown of the centrifuge once a month If the drive hole or the drive shaft is stained or any foreign matter is adhered the rotor may be improperly installed and come _ off during operation Section 4 2 Your ultracentrifuge can be damaged if connected to a wrong voltage Check the voltage before plugging the ultracentrifuge into a power source Chapter 6 Be sure to remove the rotor from the rotor chamber when centrifuge is moved After installation and before any test run this ultracentrifuge always needs the internal check by a Thermo Fisher Scientific authorized sales service representative Chapter 6 Vil N SAFETY NOTICES Never use any material capable of producing flammable or explosive vapors Be sure to take the necessary precautions before separating radioactive toxic or pathogenic materials AN AVERTISSEMENT Ne jamais utiliser de mat riau capable de produire des vapeurs inflammables ou explosives Assu rez vous de prendre les pr cautions n cessaire s avant de t
88. function to reuse history data 3 63 3 4 2 Rotor Catalog 3 4 2 Rotor Catalog Rotor Catalog 1 Example showing how to referencing the catalog Touchscreen operation Screen displays and notes Press the Rotor Catalog button in the MENU screen In the ROTOR CATALOG screen press the field of the desired rotor type Press the lt and gt buttons to turn pages Press the desired rotor cell to open the Rotor details screen ROTOR CATALOG This catalog provides information on rotor models and rotor specifications 24 Det 2013 9 30 elhe ROTOR CATALOG screen _ Rotor 3 Vertical Rotor mys Neo Angle Rotor Fizad Angle AEE FSUL Bx39 FSOL 24N1 5 w100 t a100 50 000 50000 a7 000 100 000 wi Rotor Name Number of Tubes Tube volume ml gt Wax speed rpm Rmax em Ravatem RCF man vg RCFave xg Design dans Eml 3 64 Zonal Rotor Continuous Flow Rotor Moers he Rotor List x screen appears AALER ACFavE 211984 dat as OL as Selected rotor type 280 000 240 800 1z 460 126 O29 Rotor cell caz 006 589 829 hi i 1 12 r Peevey A screen showing x rotor specifications appears 6 50 100 000 7 18 5 ai B02 006 599 829 z 1 20 3 4 2 Rotor Catalog Touchscreen operation Screen displays and notes A Use the X button to return to The Touchscreen again displays the previous screen
89. g ends the air leak valve minutes automatically activates After defrosting ends the temperature is Operation If the vacuum pump is not operating it will controlled until the air leak valve details start operating when the defrost function activates and the chamber is vented is selected When defrosting ends the vacuum pump stops operating and the air leak valve is activated to vent the chamber Press the VACUUM button to activate Press the DEFROST button from the Canceling the air leak valve and stop defrosting MENU tab and select Normal 1 Be sure to close the door when defrosting is started after the rotor has stopped 2 Wipe the rotor chamber dry if there is plenty of moisture 3 Enabling the defrost function when the rotor is turning starts defrosting when the rotor decelerates Then the bowl is heated for 10 minutes and evacuation continues Regardless of whether the rotor is still rotating or has stopped after 10 minutes temperature control will resume if the VACUUM button is On If the defrost function is enabled set the VACUUM button to Off as soon as the rotor stops and remove the rotor 4 When defrosting is enabled in zonal operation defrosting starts when the rotor decelerates When defrosting ends after 10 minutes temperature control resumes unless the air leak valve is activated and the chamber is vented 5 The temperature rise caused by the defrosting operation 10 minutes is less than 1
90. g that the unit has been properly decontaminated and outlining the procedures used will be acceptable NOTE The Field Service Engineer will note on the Customer Service Repair Report if decontamination was required and if so what the contaminant was and what procedure was used If no decontamination was required it will be so stated WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Fisher Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROHS Thermo Electron LED GmbH Robert Bosch StraBe 1 63505 Langenselbold Germany thermoscientific com centrifuge 2014 Thermo Fisher Scientific Inc All rights reserved Delrin TEFLON and Viton are registered trademarks of DuPont Noryl is a registered trademark of SABIC POLYCLEAR is a registered trademark of Hongye CO Ltd Hypaque is a registered trademark of Amersham Health As RULON A and Tygon are registered trademarks of Saint Gobain Performance Plastics
91. gging in as a User By registering users in the instrument it will be possible to manage the operation history of users that log in For information on how to register a user refer to Section 3 4 6 1 User Management User Login Procedures Touchscreen operation screen displays and notes 2 Press the Display user field in the Run screen or Admin In the User Management screen press the field of the desired user name making sure that the pressed field turns blue Then press the LOGIN button Admin is the registered factory default for this centrifuge Enter the PIN on the on screen keypad and press the Enter button The PIN is the 4 digit number you register in Section 3 4 6 1 User Management The default PIN for Admin is 1111 If necessary change the PIN 3 2 4 Logging in as a User venene he User x Management Access level Last run screen appears Administrator 23 Oct 2013 15 45 User Management User name 02 Oct 2013 13 20 Administrator 15 Oct 2018 9 15 Super User 18 Jun 2013 14 00 The LOGIN button Cees The LOGIN LOGIN PIN screen appears I PIN field Enter button 3 14 3 2 4 Logging in as a User Touchscreen operation Screen displays and notes A The user name appears in the TIL ecm The Run screen USER area in the Run Z rpm If CT TIME m appears screen ROTOR FUNCTION ki T 8100 eS Vis v2 RCE USER area ACCEL
92. gister MOTT The run parameter button sre ae eee oe ce setting screen g2 reappears ae ee k re eed 2 Fee z _ _ __ X button ii aaa a at as fs PAs oe T Ss SS SST pO oD at Seis Ganne F E 15 0 et a ae Fea ae o The entered values are displayed in the STEP 1 to 5 fields o To correct entry errors press the STEP field you want to correct and press the Edit button to make corrections when the field turns blue 3 46 3 3 1 3 Step Mode Operation Procedures Touchscreen operation Screen displays and notes screen If they are correct press the X button and press the X button in the Program registration screen Check the entries displayed IEY kal The Program list for each step in the run me x eC Tapes on Of 8 pO parameter setting display i OEN cei N 1 Call the desired program according to the instructions in Section 3 3 1 2 Programmed Operation Procedures In this example program No 11 registered in the previous procedure is called 2 Install the rotor close the door and press the START button according to the instructions in Section 3 2 5 Operation Procedures When processing samples that are sensitive to temperature rises press the VACUUM button to obtain a high vacuum level in the rotor chamber before pressing the START button r rA X button ies J Lung i 3 ri The program RN Admin faa New registered in this example s
93. h a soft cloth being careful not to touch drops of water the sensor with your finger 5 4 6 Installation 6 Installation Installation or relocation of your centrifuge must be done by the authorized Thermo Fisher Scientific service representative Contact your local dealer or a Thermo Fisher Scientific service representative In order to use the ultracentrifuge fully and safely follow the installation instructions given below DANGER To avoid electrical shock hazards follow below when servicing the centrifuge 1 Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord Then wait at least three minutes and then remove the covers from the centrifuge 2 Be sure to turn off the POWER switch and unplug the power cord from the receptacle if your centrifuge is equipped with a power cord with a plug Then wait at least three minutes and then remove the covers from the centrifuge A WARNING Before changing the power voltage by manually selecting desired tapping on the internal transformer turn off power to the ultracentrifuge then unplug the power cord from the wall outlet Changing the voltage without doing so exposes you to the possibility of electrical shock A CAUTION Your ultracentrifuge can be damaged if connected to a wrong voltage Check the voltage before plugging the ultracentrifuge into a power source 1 Power requirem
94. he capability to keep track of the number of runs and hours of use for each rotor This information can be used to automatically notify the user of the need for deration at the end of the primary life and to preclude the use of rotors once their warranted life as defined by these parameters have come to an end To utilize this feature each time a rotor is used it must be recorded by the ultracentrifuge To do this a rotor must be initially registered in the Rotor Management software Each time the rotor is used in the Sorvall WX Ultra series centrifuge it must be selected from the Rotor Management Screen prior to starting the run Additionally should the rotor be used in other ultracentrifuges the Rotor Management information can be edited to record this usage Rotors may also be deleted from the Rotor Management Screen if they are no longer to be used If you use the rotor beyond its maximum permitted life a serious accident may occur For more information see rotor instruction manual 3 92 3 4 6 Admin Function 2 Automatic rotor deration You can register a rotor in the ultracentrifuge before use After the rotor has been registered you can utilize the rotor management feature of the ultracentrifuge each time a run is made using the rotor When the registered rotor comes close to the end of its life the ultracentrifuge indicates it by displaying a message on the screen When the rotor reaches its primary life the ultracentrifug
95. he program where the step mode operation is registered and run it according to the instructions in Section 3 2 5 Operation Procedures Start operation without changing the run parameters after calling the program Changing the run parameters will cancel the called program 1 The Program display screen in the Run screen displays the following data The remaining run time for all steps is the total TIME run time for all set steps less the time that the centrifuge has been running In this example the run has not started yet so the total 3 hour run time is displayed as the remaining run time Since this time does not include the deceleration time between different steps it does not indicate the exact remaining time Use this information as a rough indication of remaining time Current step no Total number of registered steps Eee Remain 6 45 Approx hh mm Program No Remaining run time for all steps 2 When acceleration is performed before the next step the acceleration time is included in the remaining time However time for deceleration is not counted and the time count resumes when the following step starts 3 Run parameters cannot be stored during operation when the rotor is turning Perform this operation when the centrifuge is not running 4 To combine step mode operation and RTC Real Time Control operation refer to Section 3 3 4 RTC Real Time Control operation set RTC after calling the program The cen
96. hich if not strictly observed could result in personal severe injury or possible death AN WARNING This note indicates a potentially hazardous situation which if not strictly observed could result in personal severe injury or possible death AN CAUTION This note indicates a potentially hazardous situation which if not strictly observed could result in personal injury or severe damage to the instrument This hazard alert symbol indicated together with a signal word is a reminder to emphasize important safety instructions NOTE indicates a note which has no direct bearing on personal safety e Do not perform any operation not specified in the instruction manual If any problem is found on your instrument contact a Thermo Fisher Scientific authorized sales service representative Although the safety precautions in the instruction manual and safety instructions on your instrument have been fully considered an unexpected situation may arise Observe the instructions in the instruction manual and always be careful yourself when operating this instrument J N SAFETY NOTICES o Mechanical Safety e Do not open the door while the rotor is spinning WARNING e Do not attempt to slow or stop the spinning rotor by hand e Do not incline or move the instrument while the rotor is spinning Do not place any object on the instrument or lean on the instrument e Do not attempt to unlock the door forcefully while the rotor is spinni
97. hod Thermo module cooling CFC HCFC HFC free morgen CSP ay ane Color touch sensitive LCD 65 000 colors operation Applicable rotors A rotor with an overspeed decal RCF is an acronym for relative centrifugal force 1 1 1 Specifications Sorvall WX100 Plus Sorvall WX90 Plus Sorvall WX80 Plus Width 790 mm Depth 690 mm Height 880 mm Depth with safety cover mounted 890 mm Height to the handle of the centrifuge door 925 mm Dimensions Power requirement Environmental conditions Supply required Single phase 50 60 Hz 208 220 Vac 10 20 A maximum normally 8 A 230 240 Vac 10 16 A maximum normally 7 A e Ambient temperature for operation 2 C to 40 C e Ambient Temperature for performance guarantee 10 C to 30 C e Indoor use e Altitude up to 2000 m Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C e Pollution degree 2 e Overvoltage category I The voltage to be used is the one that you specified when purchasing the centrifuge CE The Sorvall WX Plus Ultra series centrifuges satisfies CE marking requirements The CE marking is an international symbol which shows that the product conforms to EC directives Standards concerning these directives are as follows e Product safety EN 61010 1 and EN 61010 2 020 e Electromagnetic compatibility EN 61326 1 1 2 2 Description 2 1 External View of Ultr
98. hooting Be sure to read and keep in mind the following cautionary information before troubleshooting AN DANGER To avoid electrical shock hazards follow below when servicing the centrifuge 1 Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord Then wait at least three minutes and then remove the covers from the centrifuge 2 Be sure to turn off the POWER switch and unplug the power cord from the receptacle if your centrifuge is equipped with a power cord with a plug Then wait at least three minutes and then remove the covers from the centrifuge N WARNING 1 If the centrifuge rotor or an accessory is contaminated by samples that are toxic or radioactive or blood samples that are pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods 2 lf there is a possibility that the centrifuge rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the centrifuge rotor or the accessory properly before requesting repairs from a Thermo Fisher Scientific authorized sales service representative Note that Thermo Fisher Scientific cannot repair the centrifuge rotor or the accessory unless sterilization
99. is engaged and touch screen appears the waiting time indication field if you want to change it Enter the waiting time on the Ten aeren Me The time in the waiting on screen keypad press the Gaa a naan Enter button to confirm and waite press the X button Enable Example Changing waiting Ter time until economy mode Colt is engaged to 10 minutes 3 82 3 4 5 Customizing Touchscreen operation Instrument operations and notes Press the CUSTOM tab the Press the CUSTOM tab or the X button to return to the CUSTOM screen o Press the Run screen tab or MENU tab to return to the respective screen o To disable economy mode press the Auto standby button and the Disable button X button the Run screen tab or the MENU tab 1 Economy mode is enabled only when the rotor is at standstill and the vacuum pump is not operating 2 During economy mode the backlight on the touchscreen is dimmed the cooling system is stopped and no electricity is supplied to release the door lock If the door is locked when the centrifuge enters the economy mode the door is locked and cannot be opened 3 To temporarily cancel economy mode touch the touchscreen This will enable all operations and the door can be opened 4 Even if economy mode is temporarily canceled the centrifuge will reengage economy mode when the set wait time elapses as long as the rotor is at standstill and the vacuum pump is not ope
100. is instrument nor handle or store them near the instrument 2 Take all necessary safety measures before using samples that are toxic or radioactive or blood samples that are pathogenic or infectious N CAUTION Do not place containers holding liquid in the rotor chamber on the centrifuge or near the centrifuge Liquid if spilt could get into the instrument and damage electrical and mechanical components 3 1 1 Starting up the Centrifuge Before setting run parameters display the Run screen screen for setting run parameters 1 Displaying the Run screen screen for setting run parameters 1 Turn on the POWER switch Thermo SCHen tir EC SORVALL gt WX Ultra Series Centrifuge 2 The initial screen appears Initial screen 4 Oet 2013 3 30 lt a Nr Ee A a T SPEED j GT Time himm n TEMP c _ Lapse 0 SET 100 000 0 00 SET 3 00 3 The Run screen appears FUNCTION Erry oe ea Run screen Fig 3 1 1 Initial Screen and Run Screen 3 3 3 1 2 Rotor and Tube Preparations and Precautions 3 1 2 Rotor and Tube Preparations and Precautions The Sorvall WX Ultra series centrifuges allow you to balance samples by eye The difference between meniscus levels of opposing samples must be less than 5 mm Fig 3 1 2 but some rotors are exceptions However some tube and bottle may in certain rotor and sample combinations trigger an imbalance alert
101. l D lakera S screen button you want to verify and press the MENU m button after the folder vLOL H Lung turns blue rl rea Admin b 2 Press the Reference NR No 0008 The Check registered button in the MENU STEP u E AEEA parameter screen B appears field oom ps _ Press the Select button 2 _ O _ _ if the target program ae Fare n es E ei ray appears Sean OE Ss Se 5 a cits aaa 5 SS t SST EE _ es i Select button am o Ifthe program that appeared is not the program you wanted press the X button to return to the Program list screen and check a different folder Do not change the run o Operate the centrifuge according to the instructions in parameters after calling the Section 3 2 5 Operation Procedures program o Reentering changing the run parameters for example SPEED TIME etc after calling a program will cancel a called program NOTE To combine programmed operation and RTC operation refer to Section 3 3 4 RTC Real Time Control operation set RTC after calling the program When a program includes step mode operation the run time of all steps must be calculated to compute RTC start time since the program cannot be called once RTC is set 3 40 3 3 1 3 Step Mode Operation Procedures 3 3 1 3 Step Mode Operation Procedures This centrifuge is provided with a step mode operation function that c
102. lect the rotor you want to register press the MENU button and press the Edit Hours button in the menu box From the Rotor log check the total run time the rotor was used in the other centrifuge enter it and press the Enter button If usage data has already been entered add the usage data for the other centrifuge Use the button to enter decimals A value that is lower than the currently registered time cannot be entered Press the Run screen tab to return to the Run screen Rotor Management S TEPSAVERTOVE S N 0000001 STEPSAVERTOVE S N 0000001 3 96 Krom Hours The Number of runs field appears The Rotor management screen reappears and the entered number of runs is displayed in the total number of runs indicator in the field of the selected rotor Entered total number of runs The Total run time entry screen appears The Rotor management screen reappears and the entered time is displayed in the total run time indicator in the field of the selected rotor Entered total run time o The display again shows the Run screen 3 4 6 Admin Function 3 Procedure for deleting a registered rotor Touchscreen operation screen displays and notes Press the Rotor management B Se esssstatses ore The MENU box button select the rotor to ko s appears in the Rotor management screen delete and press the MENU button S N l sa455
103. m the ultracentrifuge First open the chamber door then remove the rotor according to the following procedure 1 Check that the rotor is at rest Listen carefully for any sounds coming from the drive AN WARNING It can take more than three hours for the rotor to come to a complete stop because the rotor chamber is under vacuum Before opening the door wait until the rotor comes to a stop 3 103 3 5 Happenings when Power Failure Occurs 2 Turn off the POWER switch and power circuit breaker for the ultracentrifuge 3 Remove the four M5 hexagon head bolts fixing the front cover from both sides and pull down the front cover by pulling its lower side forward Then remove the front cover The upper side of the front cover is an insertion type Top deck Cabinet cover 5 0 o o0 o Ons o M5 hexagon head bolt for fixing the front cover 4 pcs fe ny E ae lt 4 Front cover Fig 3 5 1 Removing the front cover 4 Open the air vent by turning the vacuum release screw on the left of the vacuum chamber counterclockwise to let air into the rotor chamber When the pressure in the rotor chamber reaches atmospheric pressure do not forget to tighten the vacuum release screw as it was before See Fig 3 5 2 5 Pull the door unlock wire on the right of the vacuum chamber and at the same time push the door handle Then the door opens When opening the door confirm that the ro
104. m you want to call and press the Enter button Arun parameter called from the program screen Arun parameter called from the program 3 37 3 3 1 2 Programmed Operation Procedures Touchscreen operation Screen displays and notes o The number of the called program is displayed in the Program display screen o It allows you to set and display run parameters SPEED TIME TEMP ACEL DECEL ROTOR USER o The Program display screen displays the following data Total number of Current step no registered steps eS OC cena 8 Program No Remaining run time for all steps For details regarding step mode operation refer to 2 Step Mode Operation Procedures in Section 3 3 1 3 Step Mode Operation Procedures Do not change the run o Operate the centrifuge according to the instructions in parameters after starting a Section 3 2 5 Operation Procedures i Reentering changing the run parameters for example SPEED TIME etc after calling a program will cancel a called program 3 38 3 3 1 2 Programmed Operation Procedures 2 If the registered program no is not known Step Touchscreen operation Screen displays and notes 1 Turn on the centrifuge The Run screen appears POWER switch The Function Press the PROGRAM 2013 9 30 button in the Function fie Soe on ee J Lapse changes to the Selection Area Program display screen Program display ACCEL DECEL ACCEL g DECEL
105. mber equals the atmospheric pressure the temperature of the inside of the rotor chamber is kept at 25 C to prevent condensation Press this field to select the desired rotor Press this field to set the acceleration and deceleration rate ACCEL Displays acceleration modes 1 through 9 along with O DECEL Displays deceleration modes 1 through 9 along with O and free coasting F Press this area to select the desired user see section 3 4 6 Press this button to turn the vacuum pump on or off When the vacuum pump is turned off the pressure in the rotor chamber will change to equal the atmospheric pressure While the rotor is rotating you can not turn off the vacuum pump Temperature control starts as soon as the vacuum pump is turned on 2 2 1 Touchscreen and External Connection Functions of the Run screen Name and symbol Functions and actions VACUUM button START button STOP button iv STOP Function Selection area PROGRAN button h RCF button RCF wT button RTC button The following four stages are displayed depending on the vacuum of the rotor chamber Atmospheric state The vacuum pump is not active Low vacuum The rotor waits at 4 000 rpm until the vacuum reaches medium level 1 Medium vacuum _ rer High vacuum The orange LED light blinks until the pressure in the rotor chamber equals the atmosphere pressure Now ope
106. mitations and other aspects of warranty expressed above and to the following further conditions a The instrument shall be operated only within its rated maximum speed and temperature in accordance with the instructions in this manual b The drive unit shall not be overloaded nor loaded with an unbalanced rotor or an improper rotor and it shall be free from any corrosion or rust caused by spilled sample or solution on the drive spindle or in the chamber c The drive unit shall not be modified disassembled or repaired by any party but Thermo Fisher Scientific or by a service representative authorized in writing by Thermo Fisher scientific If any defect should happen to the drive unit within the afore said warranty period and accumulated number of revolutions the defective drive unit shall be replaced at no cost to the Buyer Extended Warranties are conditional on the instrument being correctly maintained by authorized Service Representatives on an annual basis 7 1 7 Warranty for the WX Plus Ultra Series Centrifuge THERMO FISHER SCIENTIFIC EXPRESSLY DISCLAIMS ANY LIABILITY TO ITS CUSTOMERS DEALERS AND REPRESENTATIVES AND TO USERS OF ITS PRODUCTS AND TO ANY OTHER PERSON OR PERSONS FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND AND FROM ANY CAUSE WHATSOEVER ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE USE OF SAID PRODUCTS Rep
107. n alert message and decelerate the rotor to a stop 2 2 2 4 Rotor overspeed decal 2 2 4 Rotor overspeed decal Overspeed decal The overspeed decal located on the rotor base has alternating black and white bands The number of bands corresponds to the maximum permitted speed of the rotor See Fig 2 2 3 Overspeed decal Fig 2 2 3 Standard Rotor To protect the overspeed decal be sure to store the rotor on the rotor stand provided See Fig 2 2 4 Rotor stand oe tie Ca OC a Dy a yu b tera orate o ae raed Fig 2 2 4 Rotor stand N WARNING N CAUTION 2 2 4 Rotor overspeed decal The overspeed decal must match the maximum speed of the rotor because the overspeed decal is a critical component that detects the over speed of rotor If an overspeed decal that is not compatible with the rotor is attached the rotor could break resulting in damage to the ultracentrifuge When replacing the overspeed decal with a new one be very careful not to damage the overspeed decal and rotor body 2 9 3 Operation 3 Operation The centrifuge comes with a wide range of functions to support a broad range of applications A brief description of each mode of operation is given below Mit Normal operation Programmed operation Step mode operation Function Displaying and setting RCF Relative Centrifugal Force w T operation Brief description Speed You can save se
108. n off the POWER switch and unplug the power cord from the receptacle if your centrifuge is equipped with a power cord with a plug Then wait at least three minutes and then remove the covers from the centrifuge N WARNING 1 If the centrifuge rotor or an accessory is contaminated by samples that are toxic or radioactive or blood samples that are pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods 2 If there is a possibility that the centrifuge rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the centrifuge rotor or the accessory properly before requesting repairs from a Thermo Fisher Scientific authorized sales service representative Note that Thermo Fisher Scientific cannot repair the centrifuge rotor or the accessory unless sterilization or decontamination is completed 3 It is your responsibility to sterilize and or decontaminate the centrifuge rotor or parts properly before returning them to a Thermo Fisher Scientific authorized sales service representative In such cases copy the decontamination sheet at the end of this manual and fill out the copied sheet then attach it to the item to be returned Thermo Fisher Scientific may ask you about the treatment for the centrifuge roto
109. n the door is displayed see below after the pressure in the rotor chamber equals the atmosphere pressure Now open the door If the sample is sensitive to temperature increases press NOTE the START button when the chamber is at a high vacuum level Press this button to start rotor rotation lf VACUUM is off pressing this button starts the vacuum pump and starts temperature control Press this button to stop rotor rotation This centrifuge incorporates a number of features such as step mode operation and RTC Real Time Control operation Buttons for these features are displayed and specified in the Function selection area Press this button to select the step mode operation etc see section 3 3 1 Press this button to display and set RCF see section 3 3 2 Press this button to set w T see section 3 3 3 Press this button to select the RTC Real Time Control operation see section 3 3 4 2 2 1 Touchscreen and External Connection Functions of the Run screen Name and symbol Functions and actions On screen keypad Use the on screen keypad to enter numeric values for run parameters When entering a time Switches from hours to minutes Hold When entering the operation time Sets continuous running FREE When entering deceleration conditions Sets free coasting When entering the deceleration CE Press this to cancel input for example if you enter the wrong rate FREE
110. n the rotor when it is turning Perform this operation with care and be sure to follow the instructions in the instruction manual Section 3 4 3 The cap is removed by hand from the rotor and the seal assembly is installed when the rotor is turning Perform this operation with care and be sure to follow the instructions in the instruction manual Section 3 4 3 Never conduct operations in a manner other than as described in this operation manual Section 3 5 When the centrifuge will not be used for a long time keep the power breaker off Section 3 5 Do not perform any operation not specified in this manual If any problem is found on your centrifuge contact a Thermo Fisher Scientific authorized sales service representative Chapter 4 and Chapter 5 Using cleaning or sterilization method other than recommended in this instruction manual may cause corrosion or deterioration of this centrifuge Refer to chemical resistance chart attached to the rotor or contact Thermo Fisher Scientific Chapter 4 For sterilization of the surface of the centrifuge and the rotor chamber wipe them with a cloth dampened with 70 ethanol Using the method other than the above method might cause corrosion or deterioration of the centrifuge Refer to the chemical resistance chart provided with the rotor or contact Thermo Fisher Scientific While we recommend that 70 ethanol is used for sterilization no guarantee of sterility or disinfection is
111. ncel RTC press the STOP button or press the RTC field and press the Cancel button in the RTC setting screen 3 When logging in as a user refer to Section 3 2 4 Logging in as a User pressing the Cancel button in the RTC screen will display the PIN entry field shown below Enter your login PIN or the administrator PIN in this field Plaase input login User FIN or atninistrator PIN 4 To combine programmed operation including step mode operation and RTC operation set RTC after calling the program The centrifuge totals the run time for all programmed operation steps to calculate the RTC start time Programs cannot be called after setting RTC RTC operation is not possible when step mode operation includes a 0 rpm step 5 Press the STOP or Cancel button to stop a run during operation or standby This cancels RTC operation and stops the rotor 3 57 3 4 Features of the MENU Screen 3 4 Features of the MENU Screen These features have been designed to make your Sorvall WX Ultra series centrifuge easier to use by including for example the Run History function shown in Fig 3 4 The MENU screen can be displayed by pressing the MENU tab on the touchscreen MENU tab 24 Oct 2013 9 30 24 Oct 2013 9 30 DEF 4 a DEFROST m Customize Manager ID Contact Admin Total number of runs for this centrifuge Total Rev 5120 x 10 Fig 3 4 MENU screen The functions of the MENU screen i
112. nction Selection Area in the Selection Area Run screen changes to the RCF display screen Y RCF display screen ACCEL DECEL 3 ra o The RCF button is not enabled when no rotor has been installed The RCF display screen cannot be accessed o The RCF display screen displays the following data RCFmax setting value Current RCFmax value RCFmax x RCFavg RCFavg setting value Current RCFavg value 3 49 3 3 2 RCF Display and Setting Function The RCF setting screen appears MN SPEED display XN Calculated RCFavg RCFmax value o The RCF setting screen displays the RCFmax and RCFavg values computed from the currently displayed SPEED value and the rotor set in step 1 Press the field of the item you IES The set value turns want to set RCFmax xg RGFavgtsq blue indicating it is waiting for user In this example 475 000 x g will be set as the RCFmax value that is displayed when the RCF field is pressed Press the on screen keypad to The entered value is enter a setting value displayed in blue SET SPEED On screen keypad Enter button 3 50 3 3 2 RCF Display and Setting Function Touchscreen operation Screen displays and notes Check the entered value and REF T 8100 The entered value press the Enter button FoFmaktxg pcFavgt xg is confirmed SET 475 000 SPEED display Calculates and displays the RCFavg value from the entered RCFmax and the rotor set in step 1
113. ng e The centrifuge itself may move if the rotor fails during high speed rotation Make sure there is a 30 cm area around the centrifuge that will allow for such movement and do not allow individuals to enter into that area during operation Also do not place dangerous objects such as flammable or explosive materials on top of the centrifuge or in the surrounding area e The overspeed decal must match the maximum speed of the rotor because the overspeed decal is a critical component that detects the over speed of the rotor If an overspeed decal that is not compatible with the rotor is attached the rotor could break resulting in damage to the ultracentrifuge e Repairs disassembly and other modifications to the centrifuge are strictly prohibited unless performed by a Thermo Fisher Scientific authorized sales service representative e Do not use the other s manufacturer s rotor without Thermo Fisher Scientific s permission e Check the chemical resistance chart attached to the rotor and do not use any sample inapplicable to the rotor including the buckets Using such a sample could corrode the rotor including the buckets e Do not exceed the allowable maximum rated speed of the rotor or buckets in use e Do not use corroded scratched or cracked rotor buckets and assemblies Check that the rotor buckets and assemblies are free of such abnormalities before operation e When using a swing rotor check that the buckets are properly engage
114. ns up to step 16 are only required when an RCF value is set PROGRAM Wo 0008 STEP 1 ACh ave 599 800 ie raidan TEMP 10 0 Wy The run parameter entry display screen reappears The SPEED TIME TEMP display fields and the on screen keypad appear Selecting a rotor in step 4 Loa 5 will make it possible to compute and display RCFmax and RCFavg values from the set SPEED value The ACCEL DECEL setting screen appears Enter the desired code No and press the Enter button The Enter button is replaced by the OK button Check the setting and press the OK button The run parameter entry display screen reappears For details see Setting acceleration and deceleration mode in Section 3 2 1 Setting Run Parameters JL To set an RCF or w T value proceed to step 14 and step 17 respectively Wo DIOE STEP 1 uN iat TEMP 10 Q The run parameter entry display screen reappears PROGRAM ACCEL z DECEL 5 To set an RCF value the rotor must first be set Set the flow as described in step 5 If a rotor has not been set the operation will not be accepted 3 28 3 3 1 1 Program Registration Procedures Touchscreen operation Screen displays and notes 15 Press the display area of the item you want to set and enter the desired value Example RCFmax 513 700 xg 16 Check the set value and value computed from the set value and press the OK button 17 To seta w
115. oks protruded from the frame and then secure it with the six M4 binding screws See Fig 6 2 Safety cover shipping status Hook Top deck M4 binding screws 4 pocs accessories M4 binding screw 2 pcs Safety cover M4 binding screws 2 pcs mounting screws for safety cover during shipping Fig 6 2 Securing the safety cover 4 Connecting power cord N WARNING Your ultracentrifuge must be grounded properly N WARNING Do not touch the power cord with wet hands to avoid electrical shocks All electrical connections should be carried out by a suitable qualified person 1 When your centrifuge is equipped with a three wire power cord see below plug the power cord coming from the rear of the centrifuge in the terminal of the distribution board in accordance with ANSI NFPA 70 NEC with CSA C22 1 CEC Part I or with both as appropriate The green yellow wire is a grounding wire Your centrifuge must be grounded properly Black Neutral Green Yellow ie Protective earth Black Line 2 When the plug Part No 84440401 is included in the accessories of your centrifuge and you want to connect the power cord to the wall receptacle NEMA 6 30R connect the plug and the end of the power cord For the other connection follow local electrical codes Your centrifuge must be grounded properly WARNING Do not hold the cord when disconnecting the power cord from the recepta
116. onfirming that the field of the item you want to set is red use the on screen keypad to enter the ot Ren ae ea eo current date and time 13 10 24 BE Instead of pressing a field you can use the lt and gt buttons on the on screen keypad to move the red area Press the Enter button when all entries have been made The CUSTOM screen reappears Setting at time Fig 3 4 5 7 Time setting screen 3 78 3 4 5 Customizing 8 Status display LED indicator lamp setting rae LED sets the display color brightness and lighting pattern of the status display lamp LED indicator beside the rotor chamber door to indicate instrument operating conditions The display color brightness and display pattern of the status display lamp can be set for the operating conditions described below Set as required by each operating environment 1 Available display colors brightness and display patterns Display color The following 8 selections that include 7 colors blue red green light blue yellow white pink and the lamp off setting Brightness 3 levels Lamp display patterns Solid lighting rapid blinking slow blinking fade a gradual and repeated dimming a total of four patterns 2 Available operating conditions and factory defaults Factory defaults Available operating conditions Display Display pattern Brightness Steady Slow blinking Slow blinking Red Slow blinking Slow blinking Intermediate
117. or decontamination is completed 3 It is your responsibility to sterilize and or decontaminate the centrifuge rotor or parts properly before returning them to a Thermo Fisher Scientific authorized sales service representative In such cases copy the decontamination sheet at the end of this manual and fill out the copied sheet then attach it to the item to be returned Thermo Fisher Scientific may ask you about the treatment for the centrifuge rotor or the part if the decontamination is checked and judged as insufficient by Thermo Fisher Scientific It is your responsibility to bear the cost of sterilization or decontamination If you have any question please contact to Thermo Fisher Scientific representative Note that Thermo Fisher Scientific cannot repair or inspect the centrifuge the rotor or the accessory unless sterilization or decontamination is completed CAUTION Do not perform any operation not specified in this manual If any problem is found on your centrifuge contact a Thermo Fisher Scientific authorized sales service representative 5 1 5 1 Alert Indicators This ultracentrifuge incorporates a self diagnosis feature that diagnoses the cause of any problem which may occur when you start the centrifuge or while in operation 65 1 Alert Indicators If any trouble occurs this machine gives a buzzer sound and displays an alert message in the FUNCTION area of the Run screen to warn of the trouble 24 Oct 2013
118. ormation on how to register a rotor refer to Section 3 4 6 3 Rotor Management If the name of the rotor type appears in the Rotor indicator field you can use the Cancel button to clear it A N CAUTION If you have selected a rotor in the Rotor Management screen be sure to check that the selected rotor in the Rotor Management screen and the rotor that is installed in the centrifuge have an identical serial number The rotor serial number is also displayed in the Rotor indicator field in the Run screen If the serial number of the selected rotor differs from the serial number of the rotor installed in the centrifuge you cannot control total run time and the total number of runs of both rotors When the Rotor management screen does not display the desired rotor press the Rotor Catalog button In the ROTOR CATALOG screen select the desired rotor type ROTOR CATALOG Fixed Angle otar Vertical Rotor Neo Angle Rotor In the screen displaying rotor types press the field of the desired rotor Check that the pressed field turns blue and Fixed Angle 7 SPEED FESL 6x13 5 65 000 FSOL 8x39 50 O00 3 2 3 Rotor Selection Touchscreen operation Screen displays and notes 24 Oct 2013 9 30 lhe ROTOR CATALOG Swinging Bucket screen Rotor Zonal Rotor Continuous Flow Si Rotor The screen for selecting a rotor appears 24 Oct 2013 9 46 RCP max 324142 RCFave 211 994 266 260
119. ple shows how to change registered data for program No 0008 2 3 Press the MENU button 4 Press the Change button in the MENU box and make changes as described in steps 5 to 8 in 1 Procedures for registration of run parameters 7 Det 2013 8 30 TIME hhmm i TEMP C o I O ile FUNCTION 5 PROGRAN button Folder button Program display screen PROGRAM The Program list MINE screen appears 0001 O002 Ley The selected folder is T Sato Admin P Johnsa imag g surrounded by a blue a 0006 LDL Admin 8 EEN frame f Program No _ Selected folder MENU button The MENU box appears in the Program list screen MENU box Change i Change button PROGRAM No 0008 gt The Program registration screen appears Rotor T 8100 0000001 User name REMARK The registered data is displayed on the screen Next button 3 32 3 3 1 1 Program Registration Procedures Touchscreen operation Screen displays and notes 5 To change run parameters Eerme The run parameter press the Next button mein setting display screen D S OPE steels ee SZ es Edit button Press a field in Step 1 check Moisan t a E The run parameter that the field turns blue and entry display screen press the Edit button appears o When multiple run parameters are registered step mode operation registration press
120. r or the part if the decontamination is checked and judged as insufficient by Thermo Fisher Scientific It is your responsibility to bear the cost of sterilization or decontamination If you have any question please contact your Thermo Fisher Scientific representative Note that Thermo Fisher Scientific cannot repair or inspect the centrifuge the rotor or the accessory unless sterilization or decontamination is completed N CAUTION Do not perform any operation not specified in this manual If any problem is found on your centrifuge contact a Thermo Fisher Scientific authorized sales service representative 4 1 Rotor Chamber 4 1 4 2 Drive Shaft Crown This centrifuge does not require complicated maintenance and inspection For longer and safe use of this centrifuge without trouble observe the following instructions N CAUTION Using cleaning or sterilization method other than recommended in this instruction manual may cause corrosion or deterioration of this centrifuge Refer to chemical resistance chart attached to the rotor or contact Thermo Fisher Scientific CAUTION For sterilization of the surface of the centrifuge and the rotor chamber wipe them with a cloth dampened with 70 ethanol Using the method other than the above method might cause corrosion or deterioration of the centrifuge Refer to the chemical resistance chart provided with the rotor or contact Thermo Fisher Scientific While we recommend that 70 ethanol
121. rating 5 When room temperature is high and the instrument needs to be cooled the cooling fan may continue operating even when economy mode is engaged 6 The waiting time until economy mode is activated can be set to a time between 1 and 180 minutes 7 Since the touchscreen backlight is dimmed in economy mode it may be difficult to realize that the instrument is on So be sure to turn it off when you have completed a run 8 The status display lamp LED indicator can be set to continue operation in economy mode to make it easier to see that the instrument is powered For details refer to 8 Status display lamp LED indicator setting 3 83 3 4 6 Admin Function 3 4 6 Admin Function language This function allows you to access administrator functions such as changing the display Manager Admin Press the Admin button in the MENU screen to open the ADMIN screen shown in Fig 3 4 6 This screen provides icons for controlling functions like user management and user lockout ZIS A a Rotor EI Management vain i al Lapse Remain Lapse Remain sept bls Rue 24 Oct 2013 9 30 User Management it M Speed sor ZONAL evel SPEED LAN Communication Fig 3 4 6 ADMIN screen The functions of the ADMIN screen icons are described below User management Users can be registered or deleted 1 For details refer to Section 3 4 6 1 ser Management User lockout Rotor management l
122. reen 2 2 2 2 1 Touchscreen and External Connection Functions of the Run screen Name and symbol Functions and actions Display areas SPEED area RCFarea TIME area TEMP area Rotor indicator field ROTOR ACCEL DECEL field ACCEL DECEL ACCEL 9 DECEL 9 User area a USER Admin VACUUM button These areas display various types of information The SPEED RCF TIME and TEMP areas display the current status in the upper part and the specified setting in the lower part For settings see section 3 2 1 SPEED Rotational speed indicator Upper part Displays the speed in increment of 10 rom when lower than 5 000 rpm and in increments of 100 rom when 5 000 rpm or more Lower part Used to set and display a speed from 1 000 to the maximum speed in increments of 100 rpm The lower two digits 1 and 10 positions display zeros For details on RCF see section 3 3 2 TIME Running time indicator Upper part Displays the remaining operation time If the running time is set to HOLD the upper part displays the elapsed time Lower part Used to set and display a range from 1 minute to 999 hours 59 minutes in increments of minutes and hours TEMP Temperature indicator Upper part Displays the temperature in increments of 0 1 C Lower part Used to set and display a temperature in the range from O C to 40 C in increments of 0 1 C When the pressure in the rotor cha
123. reen 3 52 ROTOR T 8100 ACCEL DECEL Pra Erpe Es w T display Press the w T display screen The w T setting field screen appears Left area Mantissa Right area Exponent 3 3 3 w T Operation Touchscreen operation screen displays and notes 5 Press the on screen keypad to enter a setting value n s When the mantissa has been CR 12 entered press the exponent So Tae ht entry field enter the exponent and press the a ie sa Enter button pn N Mantissa entry Example To enter 2 81 x rr E field 10 Press the X button on the ern oem he Run screen on screen keypad emg h TEM reappears SET 100 000 mornan o 4 T 8100 oe ee AL Computed run time ACCEL DECEL ie value ACCEL 9 SET 2 8iExp 12 DECEL 9 ro The w T display screen displays the following data Ej o mee BW w T display screen 2 81Exp 12 w T setting Current wT value value The run time 77h07min is computed from set speed and the w T value and displayed in the run time area When the w T setting value and the current w T value are identical deceleration starts and the current wT value shows the total value until the rotor stops 1 If the SPEED and TIME are reset after setting the wT value w T operation is canceled 2 Pressing the X button in the w T display screen will redisplay the Function Selection Area but the wT setting is retained and the
124. resentations and warranties made by any person including dealers and representatives of Thermo Fisher Scientific which are inconsistent or in conflict with the terms of this warranty including but not limited to the limitations of the liability of Thermo Fisher Scientific as set forth above shall not be binding upon Thermo Fisher Scientific unless reduced to writing and approved by Thermo Fisher Scientific NO CLAIM OF ANY KIND WHETHER IT IS TO GOODS DELIVERED OR FOR NON DELIVERY OF GOODS SHALL BE GREATER IN AMOUNT THAN THE PURCHASE PRICE OF THE GOODS IN RESPECT OF WHICH SUCH DAMAGES ARE CLAIMED AND FAILURE TO GIVE NOTICE OF CLAIM WITHIN NINETY 90 DAYS FROM DATE OF DELIVERY OR THE DATE FIXED FOR DELIVERY OR AS OTHERWISE PROVIDED IN THIS WARRANTY POLICY STATEMENT SHALL CONSTITUTE A WAIVER BY BUYER OF ALL CLAIMS IN RESPECT OF SUCH GOODS No charges or expenses incident to any claim will be allowed unless approved by authorized representative of Thermo Fisher Scientific in writing Goods shall not be returned to Thermo Fisher Scientific without Thermo Fisher Scientific s permission 1 2 8 Supply List 8 Supply List The items below are those supplied with the ultracentrifuge Instruction manual de Simulation CD ass y HH Compass Software Supplied in 1 liter Vacuum pump oil 45128 NEO VAC Containers MR100 8 1 Policy WARNING Because of the char acteristics of the samples likely to be processed biolog
125. responsibility to sterilize and or decontaminate the centrifuge rotor or parts roperly before returning them to a Thermo Fisher Scientific authorized sales service representative In such cases copy the decontamination sheet at the end of this manual and fill out the copied sheet then attach it to the item to be returned Thermo Fisher Scientific may ask you about the treatment for the centrifuge rotor or the part if the decontamination is checked and judged as _ insufficient by Thermo Scientific It is your responsibility to bear the cost of sterilization or decontamination Chapter 4 and Chapter 5 Unspecified repairs remodeling or disassembly of the centrifuge that is not listed in Table 5 1 is strictly prohibited by any person other than a Thermo Fisher Scientific authorized sales service representative Section 5 1 Before changing the power voltage by manually selecting desired tapping on the internal transformer turn off power to the ultracentrifuge then unplug the power cord from the wall outlet Changing the voltage without doing so exposes you to the possibility of electrical shock Chapter 6 Your ultracentrifuge must be grounded properly Chapter 6 Do not touch the power cord with wet hands to avoid electrical shocks Chapter 6 Do not hold the cord when disconnecting the power cord from the receptacle Hold the plug when disconnecting it Chapter 6 3 Indication of N CAUTION 1 When
126. rier des mati res radioactives toxiques ou pathog nes S310568 AN WARNING Do not loosen the vacuum release screw during rotation In the event of a power outage operate according to the instruction manual Z WARNING Risk of electrical shock Disconnect equipment from power supply before servicing and wait for at least three minutes AVERTISSEMEN Risque de choc lectrique D branchez le cordon d alimentation avant l entretien et attendre au This cover should only be removed for moins trois minutes 310566 power outages or other situations that are absolutely necessary Always make sure that the power source is off and follow the instructions in the manual A ATTENTION Ce couvercle doit uniquement tre retir en cas de panne de courant et d autres situations qui rendentla manipulation absolument n cessaire Assu rez vous toujours que la source d alimentation est coup e et suivez les instructions du manuel Ne pas ouvrirle couvercle dela chambre a air durantla rotation Si vous tes confront s une panne de courant veuillez vous r f rer aux instructions du manuel 3105582 CHINN OC CCN eT lan aear tutu TOO OOOOOCOONOON CE Aj o Table of Contents Mi Specifications T E E E E T E I E E T E E E E nese E T T 4 1 2 Description a TOE T E E A ie a S E AA ET 2 1 2 1 External View of Ultracentrifuge ee ee ee ee ee 2 1 2 2 Structure ee ee ee ee ee ee ee ee ee ee ee ee
127. ries Centrifuge 7 Warranty for the Thermo Scientific Sorvall WX Plus Ultra Series Centrifuge THERMO FISHER SCIENTIFIC MAKES NO WARRANTY OF ANY KIND EXPRESSED OR IMPLIED INCLUDING THAT OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE EXCEPT AS STATED IN THIS WARRANTY POLICY STATEMENT Subject to the exceptions and upon the conditions specified in this Warranty Policy Statement Thermo Fisher Scientific warrants each Sorvall WX Plus Ultra series centrifuge instrument to be free from defects in material or workmanship for a period of one 1 year from the date of installation of any such instrument Thermo Fisher Scientific agrees to correct either by repair or at Thermo Fisher Scientific s selection by replacement any defects of material or workmanship which develop within one 1 year after installation of any such instrument provided that investigation and or factory inspection by Thermo Fisher Scientific discloses that such defect developed under normal and proper usage The exceptions and conditions mentioned above are the following 1 Some components and accessories by their nature are not intended to and will not function for the warranty period If any such component or accessory manufactured by Thermo Fisher Scientific and part of the instrument sold fails to give reasonable service for a reasonable period of time Thermo Fisher Scientific will at its selection replace or repair such component or accessory What constitut
128. s to adjust screen brightness Press lt to darken the screen Press gt to brighten the screen Press the CUSTOM tab or the X button to return to the Disable CUSTOM screen and confirm the settings Fig 3 4 5 4a Backlight setting screen When the dimming backlight mode is disabled 2 Setting dimming backlight mode The dimming backlight mode allows you to dim es unnecessary touchscreen lighting for example when ECOM 5 i neout ALT ad you are not using it or during operation D A wi n gyPinming even while Each press of the button either enables or disables this function operation Fig 3 4 5 4b Dialog box that appears Enabling dimming backlight mode opens the dimming When dimming backlight mode is enabled backlight mode dialog box shown in Fig 2 4 5 4b for setting the time when dimming mode will be engaged 3 74 3 4 5 Customizing Pressing ___ min when the dimming backlight mode is enabled opens the screen shown in Fig a a aa 3 4 5 4c that allows you to set the time when the Dim backlight timeout setting dimming backlight mode will be engaged You can set a time between 1 and 180 minutes in one minute increments if you do not touch the screen for the set time period the backlight will be set to its darkest level Touching the screen in dimming backlight mode will return normal brightness to the screen adjusted in step 1 Pressing Dimming only while stop will
129. s to display only programs set in red folders on the screen Yellow folder button Press to display only programs set in yellow folders on the screen gaz Green folder button Press to display only programs set in green folders on the uga New folder button Press to register new programs aii Previous page button Press to display the previous a page of the program list Use the lt lt button to move several pages back _ Next page button Press to display the next page of the a program list Use the gt gt button to move several pages ahead Program list display Displays the program number Displays the set number of steps Displays the user name or remark Displays the folder design Depends on color of program folder 3 24 3 3 1 1Program Registration Procedures Press the New Folder button To register the folder press the Enter button To select a different number use the on screen keypad to select the desired number and press the Enter button The Enter button will not accept the entry of an already registered program No Press the white area in the rotor model display registration field 1 to select a rotor You can omit this operation Touchscreen operation Screen displays and notes New Program The Program No entry screen appears Program No entry field The unregistered program with the lowest number is displayed when the s
130. se of rotors other than zonal rotors will lead to small deviations in temperature indication If you intend to use the TCF 32 continuous flow rotor a black painted rotor for zonal operation contact a Thermo Fisher Scientific sales service representative 1 Zonal Operation Procedures Step Touchscreen operation Screen displays and notes Prepare zonal operation as described in the Zonal rotor instruction manual NORMAL button ZONAL Stop button 2 Press the ZONAL setting In screen TAN AEOEUESEEN Zonal setting button in the MENU screen EON SERE NE Ea screen appears ROIT TT aS Zs _ SAE l ZONAL button 3 66 3 4 3 Zonal Operation Procedures Touchscreen operation Instrument operations and notes 3 Press the ZONAL button in eee Th jd The Set Validation the ZONAL setting screen Validation No No screen appears 4 Enter 30 for the PIN on the jn Sener TN on screen keypad and press ZONAL Setting enabled when the he TE b ZONAL button is the Enter button surrounded by a FT FNE yellow frame 3 0 Enter ma NORMAL 5 Press the Run screen tab lara WEEER The Run screen IME hh appears Ce oo FUNC DON aa D000001 F ACCEL IDECE _ ME J Zonal operation En See oha USER Admin Wi o The ZONAL tab is added to the Function Selection Area and the zonal operation chart is displayed o The MENU screen will reappear if the MENU tab or the X button is pressed instea
131. ss the X button in the program display screen 20 Check the setting and press the Register button When a normal operation is registered PROGRAN The Program list me e me screen appears T Sato GEL IP Johnso a fv n n 0004 0005 F l Lung 0007 0008 ag A e E o gt manana Vma Program registered in this example When a step mode operation is registered PROGRAM No 00068 T 8100 Tee erneer 100 000 ie ao sji a g0 ooaj 1 00 E 40 ajf T sf i o a pi 6 30 e amin z a Sa ee e _ _ a coj dT Sat if _ _ 1a _ _ er re of a i Ew o The Check registered condition screen appears X button o Pressing the X button in the Check registered parameter screen opens the Program list screen eter he Run screen reappears om hh mm Lapse The Function Selection Area reappears in the Program display screen 3 31 3 3 1 1 Program Registration Procedures 2 Procedures for Changing Program run parameters The following describes how to change run parameters for registered programs Touchscreen operation screen displays and notes 1 Press the PROGRAM button in the Run screen in the Function Selection Area Press the Folder button in the Program display screen and press the No of the program in the program list you want to change The exam
132. ssage delete this user Press the YES button to o Enter the PIN of the user you want to delete and press the open the PIN field Enter button o The user field will be deleted when the User management screen is opened the next time 3 88 3 4 6 Admin Function Touchscreen operation screen displays and notes 4 2 Press the Change the Access level button to change the access level Press the access level you want to change to open the PIN field 4 3 Press the Change the PIN Pui Sereen TET eke The field for entering button to change the PIN Current PIN the current PIN appears Loree The Select access User Wanagement level field appears User name Access level Last run Administrator 23 Oct 2013 15 45 02 Get 2013 13 20 PF Johnson Administrator 15 Oet 2013 9 15 Please select the Access level Administrator Access level dialog box o Enter the user PIN you want to change and press the Enter button o The access level of the user field will be changed when the User management screen is opened the next time 1 A field for entering the current PIN appears Enter the current PIN and press the Enter button EAEEREN A field for entering a new PIN appears 2 Ascreen for entering a new PIN appears Enter the new PIN and press the o Pero A field for checking the Enter button Bed AEA new PIN appears 3 A field for confirming the PIN appears Enter the PIN ent
133. t pce cea E rai swear ltr a ana i ima Siar wel ah eter wha a an 3 90 3 Rotor management CTC eT ee eee Te eT eR ee ee a eee re er ee eRe ee ee cere eat 3 92 4 Run time indication ee A SR RR ee Oe a ee E ee ee ee re ee ee Re ee 3 98 5 Actual run timer srretrtreee tree terrence eeer ee eer eerie 3 98 6 Vacuum level Lech eT eee ee Se ee eee ee ee Ce ree ene ere ea en er eee ee eee enn ace ee arn 3 99 7 Zonal speed st ertett treet eee ee ten ttre tener eter tere 3 99 8 LAN communications lt 111rttrrtttttr etter teeter terre 3 100 3 4 7 Instrument ID Service Contact Information ay ereicaratannier aria oa tal da ae wer er a ar wer atc eyes a aterm 3 101 3 5 Happenings when Power Failure Occurs Scale safaneivan eure Beet arava a a aa A a aim snctaudsatecelahe 3 103 4 Maintenance seul tice AA ANE AE ac carta E AEE chs ete E AE ronda debs N E e E AAEE hE EEE E DEE AEE EE E AT 4 1 4 1 Rotor Chamber ee eT eC ee E E E E A a eee eet ATE ee ee a E E a are ee 4 2 4 2 Drive Shaft Crown U eA eo eR ee ee Le a E E S 4 2 4 3 Cabinet EEE ER a Ae Ree ere ee aoe ae Ree LA A A a A a R a L E A Ar 4 3 4 4 Others A acai E E EE E A as cme arn A Sve al 2k Re A E E E A E A E ae Sosa E A E E A E E A aa E ER ae 4 3 5 Troubleshooting a tue eve hao ve ice Mica rte Pee dais a ange Pig ns a rae a ee Ba a ea 5 1 5 1 Alert Indicators Teeter TP reer ee a ee ee eee eee ee ee a eee ee aa erences 5 2 5 2 Diagnosed Problems Requiring Maintenance het eat sees Sea eae hahaa
134. t indicates that this rotor reaches the final life after it is operated for twenty times or 100 hours or the less Rotor Life 2 Rotor Life 3 Rotor Life 4 1 The rotor life almost reaches the primary life When a Rotor life 2 alert signal occurs it indicates that this rotor reaches the primary life after it is operated for twenty times or 100 hours or the less 1 Information of the registered rotors is too much to enter the data on the additional rotors 1 The rotor life almost reaches the final life secondary life 5 3 and hours on the Rotor Management screen Do not use the rotor any more when it reaches the final life Refer to the instruction manuals of each rotors e Check that the total number of runs and hours on the Rotor Management screen If the rotor life has reached the primary life contact a Thermo Fisher Scientific authorized sales service repre sentative to inspect the rotor e Register the additional rotors after deleting unnecessary registered rotors on the Rotor Management screen e Do not use the rotor any more when it reaches the final life Scrap this rotor 5 2 Diagnosed Problems Requiring Maintenance 5 3 User Corrected Problem If any alert message between E11 and E86 appears this ultracentrifuge requires maintenance by a service representative When ordering a repair inform us of the alert code you have received NOTE When the E13 Unexpected MPG pulse al
135. t run parameters in memory for later use in repeated operation Memory gt Call Normal operations can be combined in a sequence of operations N The centrifuge automatically computes RCF values from set speed or speed from set values and then displays the result of the computation on the control panel IY Time RCF gt _ The centrifuge computes run time from speed and sets the w T value Time 3 1 Reference La Section 3 2 Basic Operation EE Section 3 3 How to Use the Function Selection Area Section 3 3 1 Programmed Operation LF Section 3 3 1 3 Step Mode Operations Eg Section 3 3 2 Displaying and Setting RCF BE Section 3 3 3 w2T operation 3 Operation This function is used to perform a run between a set start time and end time ty aoe set Section 3 3 4 RTC RTC operation Press the run time SPolauen Real Time Control START button Function 3 31 PM5 00 4 1 AM9 30 Time Special operation An operation that uses a zonal rotor SE Section 3 4 Features of the MENU Screen Zonal operation Section 3 4 3 Zonal Operation Procedures Zonal speed 3 2 3 1 Run Preparation 3 1 1 Starting up the centrifuge 3 1 Run Preparation A WARNING 1 This centrifuge is not explosion proof centrifuge Never use explosive or flammable samples or materials that chemically react vigorously Do not centrifuge such materials in th
136. t to set If the color of the initial digits are blue go to step 3 Press the on screen keypad to enter a numeric value Each new digit that is entered is scrolled to the left eb p a gt O O O p u Q Cc Q prar 4 Ses ab Q O Check the entered value To continue making other settings press the field of the desired setting When complete press the Enter button Use the CE button to cancel incorrect entries Setting range and units 100 000 rpm 000 100 000rpm 2 2 hours 30 minutes 30 2 hours 30 minutes Color Blue Color Blue The last two digits are fixed Press the button to move the cursor to the minutes position To start a continuous run press the Hold button 0 SET 100 000 SET 20 0 Set it to 2 30 2 hours 30 minutes Set it to 100 000 Set it to 4 C TIME hh mm SET 100 000 Set a value between 1 000 rpm to maximum speed in 100 rpm increments Set a value up to 999 hours and 59 minutes in 1 minute increments Temperatures in the 0 to 40 C range can be set in 0 1 C increments 3 8 3 2 1 Setting Run Parameters Setting acceleration and deceleration mode Settina Item Acceleration Deceleration 3 ACCEL DECEL 1 essen ACCEL 5 DECEL 5 Press a field to display the on screen keypad Press the field whose value you want to set The numeric values are displayed in blue Color Blue co
137. terns for the status display lamp Step Touchscreen operation Instrument operations and notes 1 Press the LED button rose LED 2 Press the Set pattern button for the operating condition you want to change Example To change from the Steady lighting pattern to the fade pattern 3 Press the Select pattern button for the desired pattern in the pattern palette Then confirm that the selected button is surrounded by a white frame before pressing the SET button You must press the SET button to complete the setting 4 o Press the CUSTOM tab the X button the Run screen tab or the MENU tab NOTE LED Indicator Setting rop MU WHITE BLINKING Ho I sly BLUE BLINKING LE pa J BLUE Pattarn Pallet STOP LIGHTING FADING JBLINKING BLINKING SLOW FAST z BLINKING H agw O BLUE BLINKING o H sLow ae Lok show The LED indicator setting screen appears Set pattern button The Pattern palette screen appears Select pattern button SET button The LED indicator setting screen appears Lighting test button Set pattern screen BLINKING L_ SLOW BLINKING SLOW o The appearance of the Set pattern button changes to the pattern selected fade in the pattern palette screen o The Lighting test button to the right changes to an object in a double orange colored frame and the status display lamp LED indicator is test
138. the STEP you want to change and press the Edit button Press the field of the run o To add new run parameters press the empty area STEP 2 parameter you want to in this example after the registered STEP and press the change and change the Edit button registration as described in steps 10 to 18 in 1 Procedures for registration of run parameters Check the changes and press the Register button NOTE NOTE 1 When changes are registered the previous run parameter is deleted and the changed parameter becomes effective 2 Run parameters cannot be recorded during operation when the rotor is turning Perform this operation when the centrifuge is not running 3 33 3 3 1 1 Program Registration Procedures 3 Procedure for deleting programs The following describes how to delete registered programs Touchscreen operation screen displays and notes 1 Open the Program list SROGRAM The program list screen and press the ra Li a appears and the program folder you want to 5 Sato fa al D fo soins ce lili delete as described in step 1 6 in 2 Procedures for fe _ Lune Changing Program run parameters Program No The example shows how to gt me i MENU button delete program No 0008 Press the MENU button a The MENU box appears in the Program list screen x I m JA Are your sure you want to delete this program Jd z i a Pr
139. the TIME iy Tite comin value 2 or TEMP 3 setting display field 4 a 5 a 3 TEMP setting value value display fields and display field make similar settings Isplay tie 4 ACCEL setting value display field DECEL setting value display field 6 RCFmax setting value display field 7 RCFavg setting value display field 8 w T setting value display field 5 Enter SPEED TIME and HARE GRE GE a Er aE ees TEMP run parameters Tn a a iia A E a on screen keypad Example 10 00 yp SPEED 100 000 rpm SET 10 00 TIME 10 00 TEMP 10 0 C 1 ASPEED entry is not required when an RCF o Press the display field of the item you want to set enter run value is set parameters using the on screen keypad and press the 2 A TIME entry is not Enter button required when a w T o The Enter button is replaced by the OK button value is set o For details refer to Section 3 2 1 Setting Run Parameters 3 27 3 3 1 1 Program Registration Procedures Touchscreen operation screen displays and notes 12 Check the entry and press the OK button 13 Press the white area in the ACCEL setting display field 4 or the DECEL setting value display field 5 and set the acceleration and deceleration mode Example ACCEL 5 DECEL 5 14 To set an RCF value press the white area in the RCFmax setting display field 6 or the RCFavg setting display field 7 Operatio
140. the value here is 355 000 x g Calculates and displays in the SPEED display the SPEED from the entered RCFmax value and the rotor set in step 1 the value here is 355 000 x g Use the same procedure as for RCFmax to set RCFavg Press the X button to return TIEN A The Run screen to the Run screen mie diti perating condition OM teem dl display field ae EEE Setting value display ROTOR field T 8100 BET 475 000 c DECeL S i screen fo coe __ ee Es z p RCF display o The RCF display screen shows the set RCFmax RCFavg values o The SPEED setting value display field sets and displays the value 76 900 rpm calculated from the RCF value 3 51 3 3 3 w T Operation 3 3 3 w T Operation This function is used to indicate wT a value obtained by adding run time to the angular velocity squared the centrifugal effect When the same rotor is used setting the w T makes it possible to produce centrifugation that is easily reproducible Touchscreen operation screen displays and notes 1 Set the speed according to Hid aes arama the instructions in Section j i P 3 2 1 Setting run parameters i ACCEL g gt DECEL 3 a 5 Press the w T button in the 7 Function Selection Area in the i om eel Al Selection Area 2 changes to the w T Run screen 3 4 Press the field of the item you 7 The setting turns want to set aimed emmmches hamden display sc
141. this operation with care and be sure to follow the instructions in the instruction manual A dialog box appears asking whether it is OK to decelerate and stop 24 Oct 2013 13 17 Do you want to stop 7 32 rotation oe O000001 ACCEL DECEL AGCEL 9 DECEL 9 a Rl a fF ai i e l 4 USER Admin o Check that samples have been unloaded and press the YES button o The rotor decelerates and stops Remove the rotor o Gently remove the rotor when it has stopped turning CEM The ZONAL setting ERIS SERAI NE screen appears NORMAL button ZONAL Stop button o The display again shows the Run screen o The Zonal tab is hidden and the Function Selection Area reappears 3 69 3 4 4 Defrost 3 4 4 Defrost The presence of frost or moisture in the rotor chamber especially in the bowl prior to DEF a run will considerably lengthen the time it will take to reach a high vacuum level This centrifuge comes with a defrost function that heats and evacuates the bowl to quickly remove frost and moisture DEFROST Enable the defrost function in the following situations to remove frost and dry the rotor chamber 1 Defrost and dry the rotor chamber before rotor operation and after removing the rotor 2 To prevent condensation in the rotor chamber after ending a run when the rotor is removed 1 Defrost method Touchscreen operation screen displays and notes Press the DEFROST button
142. time and number of runs must be registered After registration and use in this centrifuge the rotor is used in another centrifuge Before the rotor can be used in this centrifuge again the performance results from the other centrifuge must be added to enable correct management of run time and number of runs Touchscreen operation Screen displays and notes Press the Rotor m TIE AEORNENEE The added rotor is Management button select Ena EBE displayed in the Rotor Name Krom Ru Hours a rotor to register and enter p management screen the serial number STEPSAVERTOVE S N DOO0001 Check that the field of the fun scree rotor registered in step 1 is appears in the Rotor blue and then press the Te management screen MENU button If the field of Delate Roto the rotor registered in step 1 is not blue press the field of the rotor registered in step 1 Check that the field of the Pia ARAR rotor registered in step 1 is blue and then press the MENU button 3 95 3 4 6 Admin Function Touchscreen operation screen displays and notes Press the Edit Runs button in the menu box From the Rotor log check the total number of runs the rotor was used in the other centrifuge enter it and press the Enter button If usage data has already been entered add the usage data for the other centrifuge A value that is lower than the currently registered number of times cannot be entered Again se
143. tions to the centrifuge are strictly prohibited unless performed by a Thermo Fisher Scientific authorized sales service representative J N SAFETY NOTICES N CAUTION e f the centrifuge is exposed to ultraviolet rays for a long time the color of the covers may be changed or the coating may be peeled off After use cover the centrifuge with a cloth to protect it from direct exposure o Electrical Safety A N WARNING Your centrifuge must be grounded properly to avoid electrical shock hazards N CAUTION Do not place containers holding liquid in the rotor chamber or on or near the instrument If they spill liquid may get into the instrument and damage electrical components lf the machine will not be used for a long time turn off the main circuit breaker o Safety against Risk of Fire WARNING e This centrifuge is not explosion proof centrifuge Never use explosive or flammable samples or materials that chemically react vigorously Do not centrifuge such materials in this instrument nor handle or store them near the instrument o Chemical and Biological Safety N WARNING Take all necessary safety measures before using samples that are toxic or radioactive or blood samples that are pathogenic or infectious You use such samples at your own responsibility eTake all necessary safety measures when Risk Group I materials as identified in the World Health Organization Laboratory Biosafety Manual are handled and that
144. to change the stop signal This function eae x provides a selection of six sounds including 5 tunes and an electric beep stop Signal l Pressing the selected stop signal field will sound the signal and the field turns blue Press the Enter button to store the settings To make other CUSTOM screen settings press the X button or the CUSTOM tab For Elise Fig 3 4 5 2 Stop signal setting screen 3 73 3 4 5 Customizing 3 Sound volume adjustment 1 Adjusts the sound volume of the stop signal Volume More green segments in the indicator bar means a larger sound volume The stop signal does not sound when all segments in the indicator bar are black Volume Setting Use the sound volume adjustment buttons as described below Middle Press lt to lower the sound volume Press gt to raise the sound volume Press i to mute the sound Press 1 to set full volume Press the CUSTOM tab or the X button to return to the CUSTOM screen and confirm the settings Fig 3 4 5 3 Sound volume setting screen 4 Backlight adjustment and dimming backlight mode setting siya Adjusts screen brightness we and sets the dimming backlight mode 4 j hS Backlight 1 Adjusting screen brightness More green segments in the indicator bar means a brighter screen When all segments are black the backlight is set to its darkest level Backlight Setting Middle Use the following button
145. tor is not rotating If it is still rotating immediately close the door AN WARNING Never touch the rotor while it is rotating 6 Remove the rotor After the removal close the air vent and put the front cover back to the ultracentrifuge Insert the top surface of the front cover in the bottom surface of the front side of the top deck and put the bottom surface of the front cover on the support plate of the front cover by reversing the removing procedure Push the front cover against the frame not to make a space between the front cover and the frame Then fix the front cover on the frame with four M5 hexagon head bolts 3 104 3 5 Happenings when Power Failure Occurs Handle Vacuum release screw Vacuum chamber Door unlock wre MS hexagon head bolt for fixing the front cover 4 pcs Fig 3 5 3 Installing the front cover 7 Turn on the power breaker A CAUTION When the centrifuge will not be used for a long time keep the power breaker off 3 105 4 Maintenance 4 Maintenance Be sure to read and keep in mind the following cautionary information before maintenance AN DANGER To avoid electrical shock hazards follow below when servicing the centrifuge 1 Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord Then wait at least three minutes and then remove the covers from the centrifuge 2 Be sure to tur
146. trifuge totals the run time for all programmed operation steps and calculates the RTC start time Programs cannot be called after setting RIC 5 Ifa step stores a speed exceeding the maximum allowable speed for your rotor the incorrect SPEED setting alert will be triggered Check the speed settings in all steps and correct as required 6 Press STOP button if the run has to be stopped This will stop the rotor and prevent the centrifuge from proceeding to the next step 3 44 3 3 1 3 Step Mode Operation Procedures 3 Procedures for Registering Step Mode Operation that Includes Stops Follow the instructions in 1 Registration Procedures for Step Mode Operation Perform the registration procedures described below Setting example The following describes the setup procedures for continuous operation under the five run parameters including a 0 rpm settings shown in the figure below The operations described in Fig 3 3 1 2 if performed according to this example will be performed automatically ae Step 1 Step 2 Step 3 Step 4 Step 5 15 0 C 15 0 C 15 0 C 190 C 15 0 C Acceleration mode Deceleration D a 7 gt fo fo End of run 1 hour The door 4 hour The door can be can be opened opened Fig 3 3 1 2 Example of Step Mode Operations that include stops Touchscreen operation screen displays and notes 1 As described in steps 1 to 8 in 1 Procedures for registration of run parameters in S
147. tton Space Delete Check the entry and press fun ses ri Cece The Register PIN the Enter button screen appears PIN field O Enter a PIN four numeric digits and press the L registration screen Enter button Bp sare PIN check field 3 86 3 4 6 Admin Function Touchscreen operation screen displays and notes Again enter the PIN entered tun Screen Tern Semen An Access level in step 5 and press the PIN confi rn dialog box appears Enter button dialog box o Anaccess level setting makes it possible to control access at three different levels o The user lockout function has to be enabled to allow user lockout For details refer to Section 3 4 6 2 User Lockout o The following access levels are provided Administrator Allowed to perform any operation Supervisor Allowed to set and change run parameters select programs and other operations User Allowed to perform START STOP and VACUUM operations For details refer to Fig 3 4 6 2 Operations permitted by each access level Select an access level OEE The User User Management User name Access level Last run Ei Management screen i Administrator 23 Oct 2013 15 45 reappears T 8ato 02 Get 2013 13 20 PF Johnson Administrator 15 Ost 2013 9 15 Supervisor 18 Jun 2013 14 00 _ Added user The added user name and access level are displayed again Press the ADMIN tab the X Press the ADMIN tab or the
148. urs 3 5 Happenings when Power Failure Occurs A N DANGER To avoid electrical shock hazards follow below when servicing the centrifuge 1 Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord Then wait at least three minutes and then remove the covers from the centrifuge 2 Be sure to turn off the POWER switch and unplug the power cord from the receptacle if your centrifuge is equipped with a power cord with a plug Then wait at least three minutes and then remove the covers from the centrifuge N WARNING 1 Never open the door during rotation 2 Never touch the rotor during rotation AN CAUTION Never conduct operations in a manner other than as described in this operation manual 1 Rotation of the rotor The rotor coasts to a stop If the power is restored when the current speed is more than 500 rpm the rotor will be automatically accelerated to the set speed If it is restored when the current speed is less than 500 rpm the rotor will decelerate to a stop 2 Displays on the panel During the power failure all the displays on the control panel are off When the power is restored the centrifuge will restart the control of the run with the set parameters that were in effect before the power failure battery backed and will report the occurrence of the power failure by lighting up the alert message 3 Removing the rotor fro
149. utputting and Reusing Run History Display and Run Parameters 1 The centrifuge can automatically store a run history containing up to 5 120 run O parameters The run history can be used for checking operation and for reuse R n verify user and rotor performance and output data in the CSV format History 1 Checking and reusing Run History Touchscreen operation Screen displays and notes Press the Run History button in the MENU screen 2 Press the row for the history item in the Run History you want more information on Run ise OT Details Stop Status Completed CESEN The Run History screen appears Run History 2 Date l 23 Oct 13 15 45 100000 T 8100 l Qet 13 14 25 100000 T 8100 317 Oct 13 15 20 100000 T 8100 415 ct 13 9 15 41000 TH 641 5 10 Oct 13 10 40 41000 TH 641 Page 0001 The Run History is displayed in date order Press the lt and gt buttons to page through the Run History screen page by page Press the lt lt and gt gt buttons to browse the Run History screen 10 pages at a time Use the Top button to move to the top of the Run History screen ee emees The Run History Details screen appears User Admin f Speed Set Speed 100000 lt FActual Speed 700 000 ACCEL 4 DECEL T Total Rev 11 54 x 10E Run time Load for a new run button o When a rotor is selected when loading for a new run the speed field will be displayed in gray
150. value entry and the field is display field ready to accept your next entry Setting values are displayed in the setting value display field Fig 3 2 2 Setting value display 3 6 3 2 1 Setting Run Parameters Methods for setting run parameters are described on the next page 1 If you enter the wrong value press the CE button and enter the correct value If you have already pressed the Enter button repeat the process and enter the correct value 2 When more than one SPEED TIME or TEMP run parameter is selected you do not need to press the Enter button When you touch the field of another run parameter the currently entered item is confirmed 3 To set continuous run HOLD and specify the time during operation enter the sum of time elapsed so far plus the desired remaining run time For example if the centrifuge has been running for 5 hours and you want operation to stop 1 hour and 30 minutes later touch the TIME field and enter 6 3 0 3 7 3 2 1 Setting Run Parameters Setting speed run time and temperature Operation procedures will be described using examples Setting It Speed run time Temperature eee SPEED TIME E Setting example example H Press the SPEED TIME or TEMP field to display the on screen keypad Each field displays the value set for the previous run If the color of the initial digits of the setting are white again press the field of the item you wan
151. y button in Eemu The Program No entry the MENU field and enter the screen appears number of the program you want to register Program No entry field o The unregistered program with the lowest number is displayed when the screens change o To register to another number press the program no entry field and enter the desired number on the on screen keypad 3 35 3 3 1 1 Program Registration Procedures Touchscreen operation Screen displays and notes Press the Enter button PROGRAM The Program list screen appears l Sato fun Johnsa t The Enter button will not x accept the entry of an already i ie registered program No S P 00 D er yi The copied and newly m do Ex am registered program folder o The copied program is registered under a new number 3 36 3 3 1 2 Programmed Operation Procedures 3 3 1 2 Programmed Operation Procedures The following describes procedures for programmed operations by calling registered run parameters This is convenient for using the same run parameters repeatedly 1 If the registered program no is known Press the PROGRAM button in the Function Selection Area S PROGRAM button Program display screen PROGRAM button Press the Program No HTT The Program No entry button in the Program display screen appears screen Program No entry field Use the on screen keypad to enter the number of the progra

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