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Centrifuge 5415 D / Centrifuge 5415 R
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1. 37 1 Introduction The Centrifuge 5415 D is anon refrigerated bench top centrifuge and the Centrifuge 5415 Risa refrigerated bench top centrifuge With their space saving design and user friendly operating concept these centrifuges are ideal for everyday routine tasks In both devices 24 or 36 Eppendorf micro test tubes can be centrifuged simultaneously in a 45 fixed angle rotor at 16 100 rcf 13 200 rpm The basic functions can be selected using the SHORT key and the TIME and SPEED dial The values appear in the display in digital form Before starting up Centrifuges 5415 D R for the first time please read the instruction manual The latest version of the manual and the safety instructions in your language can be found on the Internet at www eppendorf com N This sign is found on your centrifuge and on several pages in the operating manual Texts labeled with this sign contain safety notes Read these safety precautions before using the centrifuge for the first time 1 1 Delivery package 1 Centrifuge 5415 R w o rotor Order No 5426 000 018 or 1 Centrifuge 5415 D with rotor Order No 5425 000 219 or 1 Centrifuge 5415 D w o rotor Order No 5425 000 014 with 1 Main power cable 1 Instruction manual 1 Rotor key 1 Set of fuses 1 2 Setting up the device Place the centrifuge onto a stable horizontal work surface Ensure that the working
2. 1 2069 28 Changing the centrifugation parameters during the run 28 Fixed setting of the parameters only for 5415 R aa aa aaa 28 At set rpm only for SS RN Ae der cde ee 29 Short spin Centrifugation sa a ass aara nun EIN EEN wd 29 Continuous centrifugation 4552 1 ee ack 29 Switching to the ref display e EEN NEIER oins iraire ara 29 Cooling 1 5415 2 29 Switching the signal tone on and off only for 5415 2 2 30 Opening the centrifuge in the case of a power failure 30 DEVICE ES 7 7 7 b d s 30 Cleaning and maintenance 4 4 44 849 31 DEVIC 3 3 10 31 7 1 1 1 1 1 31 Asrosol tight r t rs 2 gt 22 Ne 111170 1 0 31 Rotor sterilization ic cs pn tiara a eee P eee ee i E 31 Glass breakage 4 31 Returniof the device ee ne ph 7 7 77 0 32 Troubleshooting 3 7 0 0 33 Technical data 3333 3 0 35 Ordering information
3. Centrifuge 5415 D Centrifuge 5415 R Centrifuge 5415 D R Tastenk rzel Shortcuts Task Lid Press Display Instruction 5415 R manual Display 3 4 Parameter change flashes during centrifugation closed gt 2 sec 5 sec Signal L 3 11 ON OFF T qd open gt 2 sec Parameter lock 7 AN A 3 5 ON OFF a IU open gt 2 sec At set rom L 3 3 6 E K open gt 2 sec No part of this publication may be reproduced without the prior permission of the copyright owner Copyright 2004 Eppendorf AG Hamburg Figur 1 Centrifuge 5415 D Figur 1 Rotor Power switch Time Short Speed Figur 2 Centrifuge 5415 R Figur 2 real FAST TEMP START STOP and TIME SPEED and RPM RCF Contents 22 2 1 2 2 3 1 3 2 3 3 3 4 3 5 3 6 3 7 3 8 3 9 3 10 3 11 3 12 3 13 4 1 4 2 4 3 4 4 4 5 4 6 2 23 2 2 ar 2333 3 23 Delivery package 7 7 1 50 23 21 1 7 0 23 eege te Seiden a aes ah ante rage mec urge ny ee 24 Intendedi se a 5 24 Safety precautions and applicational limitations aaa dan de 24 Operation 307 1111 9090 27 Control elemMents 2 1 1 1 11 1 1 0 27 TO e a Ra 3 27 Centrifugation with time setting 220
4. 003 5425 350 015 5425 351 003 Please use the original accessories recommended by Eppendorf Using spare parts or disposables which we have not recommended can reduce the precision accuracy and life of the centrifuges We do not honor any warranty or accept any responsibility for damage resulting from such action 37 0 01 40 Ordering information 38 7 Ordering information Rotor code All Eppendorf rotors are designated according to a simple logical system which describes the technical specifications as a uniform series of numbers and letters e g Fixed angle Angle of Tube tube adapter rotor adapter bore Adapter Swing bucket rotor bore I I I I I F A 45 30 11 A 4 81 I Aerosol tight Max no tubes Max no tubes version adapters adapters RES et ce ce ce ce e E t Ce ce ce ce c K ce Kr ce C Te ce ce ce ce KE cS CE ett ce C ce ce Ee E EG Konformit tserkl rung Ki o oi EC Confor mity Declaration E er ce C E Das bezeichnete Produkt entspricht den einschl gigen grundlegenden Anforderungen der C aufgef hrten EG Richtlinien und Normen Bei einer nicht mit uns abgestimmten nderung des t E ce Produktes oder einer nicht bestimmungsgem en Anwendung verliert diese Erkl rung ihre G ltigkeit 3 ce The product named below fulfills the relevant fundamental requirements of c vc ci 3 ce CE LC the EC directives and standard
5. REP amp SLOVAKIA EPPENDORF Czech amp Slovakia s r o Tel 420 323 605 454 Fax 420 323 605 454 E Mail eppendorf eppendorf cz Internet eppendorf cz Internet eppendorf sk FRANCE EPPENDORF FRANCE S A R L Tel 33 1 30 15 67 40 Fax 33 1 30 15 67 45 E Mail eppendorf eppendorf fr Internet www eppendort fr GERMANY Eppendorf Vertrieb Deutschland GmbH Tel 49 2232 418 0 Fax 49 2232 418 155 E Mail vertrieb eppendorf de Internet www eppendorf de HUNGARY Eppendorf Austria GmbH Tel 43 0 1 890 13 640 Fax 43 0 1 890 13 64 20 E Mail bosze z eppendorf at Internet www eppendorf hu INDIA Eppendorf India Limited Tel 91 44 42 11 13 14 Fax 91 44 42 18 74 05 E Mail info eppendorf co in Internet www eppendorf co in ITALY Eppendorf s r l Tel 390 2 55 404 1 Fax 390 2 58 013 438 E Mail eppendorfGeppendort it Internet www eppendort it JAPAN Eppendorf Co Ltd Tel 81 3 5825 2363 Fax 81 3 5825 2365 E Mail info eppendorf jp Internet www eppendorf jp NORDIC Eppendorf Nordic Aps Tel 45 70 22 29 70 Fax 45 45 76 73 70 E Mail nordic eppendorf dk Internet www eppendorf dk SOUTH amp SOUTHEAST ASIA Eppendorf Asia Pacific Sdn Bhd Tel 60 3 8023 2769 Fax 60 3 8023 3720 E Mail eppendorf eppendorf com my Internet www eppendorf com my SPAIN Eppendorf Iberica S L U Tel 34 91 651 76 94 Fax 34 91 651 81 44 E Mail iberica eppendorf es Interne
6. environment is well ventilated According to the requirements of the EN 61010 2 020 standard a safety distance of 30 cm must be observed around the centrifuge during operation No objects whose destruction may cause damage should be positioned in this space Please remove the transport safety device from the rotor chamber and keep it in a safe place in case the centrifuge should be transported in the future If you have ordered a Centrifuge 5415 D including a rotor first remove the upper transport retaining ring open the rotor lid by pulling on the shiny button Loosen the rotor with the provided rotor key and pull it off If the rotor sits tightly push the rotor edge downwards Now remove the lower transport retaining ring from which the flagging strips hang In order to prevent seal damage caused by false transport the Centrifuge 5415 R may first be switched on 4 hours following installation Mains power and frequency must be in agreement with the information given on the device identification plate Fuse protection in the device 5415 DI R 230V 2 5 A time lag 5415 D 120V 5 0 A time lag UL fuse 5415 R 120V 6 25 Atime lag UL fuse Now connect the centrifuge to the power source and switch it on on the right side Insert the rotor and screw it tight with the rotor key The centrifuge is ready to operate when the digits appear in the display field Please don t move the centrifuge while the rotor is running Check that the rotor is sitting co
7. spin centrifugation The SHORT key activates a short spin run at a preset rotational speed or at maximum speed for as long as the key is held down SHORT If the centrifuge lid is closed and you press the key for longer than 2 s the centrifuge will switch the mode to short spin at preselected or maximum speed If the centrifuge lid is open and you press the key for longer than 2 s the centrifuge will switch the mode from maximum speed to preselected speed and vice versa SHORT If the centrifuge lid is open and you press the key briefly the mode you have selected will be displayed 13t Short spin centrifugation at maximum speed 1 13t Short spin centrifugation at preselected speed ashort spin run which has already been interrupted can be continued by pressing this key if the centrifuge has not yet come to a standstill This may be done three times in total After this the lid must be opened 3 8 Continuous centrifugation TIME Using the keys set the dial to above 99 min or below 0 5 min to display 00 co The time is counted upwards in minutes STOP To end continuous operation 3 9 Switching to the rcf display SPEED If the Speed key is pressed the rpm and rcf values appear alternately in the display 3 10 Cooling only for 5415 R Using the TEMP keys it is possible to set the nominal temperature between 0 C and 40 C The nominal temperature can also be changed during the run When the nominal
8. temperature that has been set has been reached a deviation in excess of 3 C will be indicated by a flashing temperature display 29 0 Operating 30 3 Operation If the temperature deviates by more than 5 C after the nominal temperature has been reached the centrifuge switches off and err 18 appears in the display Fast TEMP starts a temperature control run at a rotor specific speed It enables the new preselected temperature to be attained rapidly in the rotor Cooling down from room temperature to 4 C takes max 16 minutes The run can be stopped automatically or by pressing the Stop key Standby cooling With the lid closed the rotor chamber is cooled down to the preselected nominal temperature before or after the run on condition that this temperature is below the ambient temperature Likewise Centrifuge 5415 R maintains the temperature after a run when the lid remains closed temperature adaptation is slow The nominal temperature appears in the display If the centrifuge is not used for longer than eight hours or if the lid is not opened for this period the refrigeration function switches off in order to protect the device The actual temperature in the rotor chamber appears in the display The desired temperature can be rapidly reattained via Fast TEMP Remove condensation water or ice by thawing from the rotor chamber at regular intervals using a soft absorbent cloth Note For prob
9. tightly closed lid 4 4 Rotor sterilization All rotors are autoclavable 121 C 20 min Aerosol tight rotor lids should be replaced every 10 autoclaving cycles 4 5 Glass breakage When centrifuging glass tubes please observe that high speeds rcf s increase the risk of glass breakage Please follow the manufacturer s instructions concerning the maximum speed rcf of centrifuge tubes In the event of glass breakage carefully remove all splinters and all ground glass from the rotor the buckets the adapters and the rotor chamber You may need to replace the rubber mats and adapters to prevent further damage Fine splinters of glass may otherwise scratch the surface of the rotors and buckets reducing their resistance to chemicals The air turbulences within the rotor chamber produce a very fine black powder of abraded metal In addition to causing damage to the rotor chamber rotor buckets and adapters the powder also contaminates the samples Check the rotor bores regularly for residues and damage 31 4 pue Cleaning and maintenance 32 4 Cleaning and maintenance 4 6 Return of the device When returning centrifuges please ensure that the devices have been fully decontaminated and do not thereby present a health risk to our Service staff You will find additional information and a blank of the decontamination confirmation at www eppendorf com Y
10. ORT Short spin key FAST TEMP Preliminary cooling run key only 5415 R SPEED Turn speed selection dial RPM RCF Press speed selection dia OPEN To open the lid only 5415 R Rotor with 24 or 36 bores for microcentrifuge tubes Rotor lid with central fastening button Mains power switch right hand side Mains power socket with fuse element 6415 D rear 5415 R right hand side Identification plate with power supply values rear E Emergency lid release for 5415 D on the base of device with cord for 5415 R with rotor key from right 3 2 Loading the rotor The micro test tubes with a nominal volume of up to 2 0 ml are loaded into the bores of the rotor The test tubes are positioned in pairs opposite each other and should each contain roughly the same amount of sample The specifications for the maximum permitted weight per bore apply to the adapter test tube contents In order to minimize air friction and noise the rotor should not be operated without the rotor lid The lid must be replaced where there are visible indications of change or damage to the lid 0 640 Operation 28 3 Operation 3 3 Centrifugation with time setting Switch on the power supply The nominal values from the last run appear in the display Load the rotor symmetrically Attach the rotor lid and close the lid of the centrifuge TEMP To set temperature preselection only 5415 R TIME To modify the
11. RPM 16 100 RCF 3 100 Nm 13 200 RPM 16 100 RCF 2 740 Nm 7 Ordering information Centrifuge 5415 D 230 V 50 60 Hz without rotor 230 V 50 60 Hz with rotor Centrifuge 5415 R 230 V 50 60 Hz without rotor Standard rotor F 45 24 11 aluminum 24 bores max 11 mm 45 angle for 1 5 2 ml microcentrifuge tubes with polypropylene lid Aerosol tight rotor FA 45 24 11 Aluminum 24 bores max 11 mm 45 angle for 1 5 2 ml microcentrifuge tubes with lid aluminum Rotor lid aluminium aerosol tight for FA 45 24 11 Adapter for 0 5 ml microcentrifuge tubes and 0 6 ml Microtainers for F 45 24 11 set of 6 Adapter for 0 4 ml microcentrifuge tubes for F 45 24 11 set of 6 Adapter for 0 2 ml PCR test tubes for F 45 24 11 set of 6 Special rotor F 45 36 8 aluminum 36 bores max 8 mm 45 angle for 0 5 ml microcentrifuge tubes with polypropylene lid Adapter for 0 2 ml PCR test tubes for special rotor F 45 36 8 set of 6 Accessories Replacement lid for rotor F 45 24 11 polypropylene Replacement lid for rotor FA 45 24 11 aluminum aerosol tight Replacement lid for rotor F 45 36 8 polypropylene Rotor key Set of fuses 5415 D R 230 V 2 x 2 5 A slow acting Important 5425 000 014 5426 000 219 5426 000 018 5425 725 000 5425 737 009 5425 740 000 5425 716 001 5425 717 008 5425 715 005 5425 730 004 5425 723 008 5425 701 004 5425 740 000 5425 733
12. ent during the rest period it is possible with the Centrifuge 5415 R to fix the desired parameter settings To do this simultaneously press the SHORT and OPEN keys for at least 5 seconds with the centrifuge lid open After 5 seconds the dial adjustment will be deactivated and the centrifugation parameters secured against unintentional adjustment The fixed setting of the centrifuge parameters will be represented by a pictogram Din the Centrifuge 5415 R The set parameters can no longer be adjusted neither during the run or in the rest period A centrifuge run can also be started or ended using START STOR It is also possible to switch the signal tones and RCF switchover on and off In order to reactivate the dial adjustment simultaneously press the SHORT and OPEN keys for at least 5 seconds The pictogram W appears in the display 3 Operation 3 6 At set rpm only for 5415 R With the Centrifuge 5415 R it is possible to start the centrifugation time countdown when the preselected rotational speed has been achieved At set rpm Pressing the START STOP key for more than 2 seconds while the centrifuge lid is open results in a switch to At set rpm mode represented by the pictogram In order to leave the At set rpm mode again and to start the centrifugation time countdown immediately after starting the centrifuge press the START STOP key again with the centrifuge lid open for more than 2 seconds until the X pictogram appears 3 7 Short
13. ion caused by commonly used laboratory chemicals by means of an electrolytic coating although this protection cannot be fully guaranteed Please ensure that the rotor is protected from mechanical damage Even slight scratches and cracks can cause severe material damage to the rotor interior Avoid damage caused by aggressive chemicals including concentrated and mild alkalis concentrated acids solutions containing mercury ions copper ions and other heavy metal ions chlorinated hydrocarbons concentrated saline solutions and phenol In the event of contamination caused by aggressive agents the rotor must be cleaned immediately with a neutral detergent Rotors caps or buckets having suffered chemical or mechanical damage or which have exceeded their maximum useful life may no longer be used Centrifuges must be correctly installed and used Repairs may be performed only by an Eppendorf authorized service technician Only original rotors and spare parts from Eppendorf may be used When transferring from a cold room to a normal laboratory the Centrifuge must either be run warm for 30 minutes in the cold room or it must be allowed to warm up for at least three hours in the laboratory before connecting it to the power source to prevent damage caused by condensation When handling toxic or radioactive liquids or pathogenic bacteria belonging to Risk Group II see World Health Organisation Laboratory Biosafety Manual observe national regulati
14. lem free operation at room temperatures below 18 C a warm up time of approximately one hour is necessary 3 11 Switching the signal tone on and off only for 5415 R Press the OPEN key for longer than 2 seconds with the lid open This switches the signal tone on or off alternately The switched on signal tone is represented by the pictogram d 3 12 Opening the centrifuge in the case of a power failure Unplug the device Wait until the rotor comes to a standstill This may take up to 5 minutes The emergency lid release of the 5415 D is activated by pulling a cord on the base of the device A small white plastic button is located in the ground plate behind the front right hand suction foot see E on the fold out page at the front of this manual Pull out this button and pull the cord vertically downwards Emergency lid release 5415 R Remove the round stopper on the right hand side of the housing Insert the rotor key provided through the bore that has been vacated The centrifuge lid is released by rotating to the left 3 13 Device fuses The fuse holder of the 5415 D is located below the mains plug The fuse holder of the 5415 R is located on the left of the main switch These holders may be pulled out backwards and the two fuses may be replaced see Ordering information 4 Cleaning and maintenance 4 1 Device The outer surfaces of the centrifuge and the rotor chamber should be cleaned once a month using a warm de
15. n Community The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2002 96 EC on waste electrical and electronic equipment WEEE According to these regulations any devices supplied after 13 08 05 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste They are marked with the following symbol to indicate this As disposal regulations within the EU may vary from country to country please contact your supplier if necessary 25 N Kyayes N Safety 26 Declaration concerning the ATEX directive 94 9 EC The present design and ambient conditions inside Eppendorf centrifuges mean that they are not suitable for use in a potentially explosive atmosphere The centrifuges must therefore only be used in a safe environment such as the open environment of a ventilated laboratory or a fully extracted fume hood The use of substances which may contribute to a potentially explosive atmosphere is not permitted The final decision on the risks in connection with the use of such substances is the responsibility of the user of the centrifuge 3 Operation 3 1 Control elements See the front view of the device found on the fold out page at the front of this manual TIME Turn time selection dial STARTISTOP Press time selection dial TEMP Temperature selection keys only 5415 R SH
16. ons If such liquids are spilled in the rotor or the rotor chamber the centrifuge must be cleaned thoroughly and properly Before using cleaning and disinfecting methods other than those specified in Chaper 4 Maintenance and cleaning please check with the manufacturer that these methods do not damage the centrifuge Before centrifugation all the tubes should be subjected to a visual inspection for material damage Damaged tubes must not be centrifuged as if tubes break there may be further damage to the centrifuge and its accessories in addition to loss of the sample A liquid density of 1 2 g ml must not be exceeded at the maximum rotational speed Sample tubes heat up over longer run times Observe the limiting data regarding the load bearing capacity of the centrifuge as specified by the manufacturer The use of organic solvents e g chloroform phenol may have an adverse effect on the stability of plastic test tubes Close the tube lids properly when centrifuging Open tube lids can be torn off during centrifugation and damage the centrifuge When closing the centrifuge lid be sure that your fingers are not beneath the lid d They may otherwise become stuck Transfer If the device is passed on to someone else please include the instruction manual Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the Europea
17. otational speed Leave centrifuge switched on and measurement system wait for 5 minutes Repeat run Error 5 Lid switch Wait for the centrifuge to come to a standstill and open the lid Then close the lid and restart the centrifuge 00 0 1 Troubleshooting 34 5 Troubleshooting Error Cause Solution Error 6 Drive error Repeat run Error 7 Overspeed Check whether rotor is positioned correctly Error 8 Rotor loose Tighten rotor Drive error Error9 25 Electronics error Repeat run Error 18 Temperature error Switch off the centrifuge only 5415 R A 5 C Error 19 only 5415 R Cooling circulation is overheated Make sure that the ventilation slit is not obstructed Error 24 only 5415 R Error with cooling aggregate e g overheating After long period of operation Allow to cool At the beginning of operation Contact Service If the solutions suggested here prove to be unsuccessful please contact Brinkmann Service at 800 645 3050 in USA and 800 263 8715 in Canada 6 Technical data Centrifuge 5415 D Power supply Power requirement Max rotational speed Max centrifugal force Max load Max kinetic energy Max density of material to be centrifuged Ambient temperature Max relative humidity Dimensions Weight without rotor Acceleration time 230 V Deceleration time Accelera
18. ou can also enquire with your person responsible for lab safety regarding a suitable decontamination method Please fill out the decontamination confirmation and place it together with the device when it is to be sent back to Eppendorf 5 Troubleshooting Error Cause Solution No display No main power Plug in mains cable on both sides connection Power failure Check main power fuse of device and of laboratory Lid cannot be opened Power failure Emergency lid release see Sec 3 12 Rotor is still running Wait for the rotor to come to a standstill Centrifuge shakes Rotor not loaded Stop centrifuge run and load rotor during acceleration symmetrically symmetrically Rotor lid does not open Stop centrifuge Close the lid correctly Centrifuge does not Lid not closed Lift the lid and close it again start The centrifuge does 1 Safety function to prevent Do not release key during restart in the Short spin centrifuge from overheating short spin centrifugation mode Flashing temperature Temperature deviation of display only 5415 R gt 3 C from nominal value Display LID Lid latch not disengaged Close lid correctly press Start Stop or activate emergency lid release see Sec 3 12 Lid latch not engaged Lift up lid slightly and close it again INT Power supply interrupted Check main plug during run Repeat run OFF Mains supply is switched off Error 3 Errors in r
19. rrectly prior to the first start 23 0 0 1 N Safety 24 2 1 Intended use The Centrifuge 5415 D R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes This device may only be operated by trained specialist staff They must have carefully read the operating manual and be familiar with the function of the device 2 2 Safety precautions and applicational limitations AN For your own personal safety please observe the following safety precautions The rotor and the rotor lid must always be correctly fastened Centrifugation may only be carried out with a tightly fastened rotor Before each centrifugation operation it is important to check whether the rotor has been tightened up to specification To do this place the rotor key supplied on the rotor nut and turn it clockwise until the rotor nut is firmly tightened up Then push the polypropylene rotor lid with the seal onto the rotor nut or screw the aluminum rotor lid on tightly The rotor must be loaded symmetrically Opposing tubes should be of the same type and should be filled equally Rotors with significant traces of corrosion or indications of mechanical damage may not be used Please inspect the accessories regularly Rotors are high quality components that must be able to withstand extreme mechanical strain Aluminium rotors are protected against corros
20. run time SPEED To modify the speed in increments of 200 rpm or in rcf START To start the run The Il symbol flashes when the rotor is running STOP To abort the run The remaining time appears in the display during the run When the time is changed during a run the time which has already elapsed is taken into account The last minute is counted down in seconds After the set time has expired the centrifuge stops automatically If the time is modified during the braking phase the centrifuge accelerates to the nominal rotational speed 3 4 Changing the centrifugation parameters during the run 5415 R Time setting rotational speed and temperature can first be changed during the run after pressing the SHORT key in order to prevent unintentional adjustment of the parameters during the run To do this briefly press the SHORT key until the display begins to blink You can set the new centrifugation parameters using the TIME and SPEED dials and the TEMP keys The new centrifugation parameters are adopted after 5 seconds 5415 D Time setting rotational speed and temperature can be changed during the run using the dials The new nominal rotational speed is adopted 2 seconds after the change A time change during the braking phase results in renewed acceleration toward the nominal rotational speed 3 5 Fixed setting of the parameters only for 5415 R In order to also secure the centrifugation parameters against unintentional adjustm
21. s listed In the case of unauthorized modifications to the product ce e por ce or an unintended use this declaration becomes invalid ce ce Produktbezeichnung Product name C Wi 98 79 EG EN 14971 EN 61010 2 101 EN 61326 2 6 Projektmanagement Project Management ce C Ss et 900 ar 1 Pa ce CGE eppendorf KA LE D CE 6 ce c er c Eppendorf AG Barkhausenweg 1 22339 Hamburg Germany te ce H H 9 Le D Se K Se ey CR ce 7 mm K Se Mt ce gt ce Ar ze ce Ce Go OE rer eS Eck CE e OS Gas ce C ce i ra CE w e CE ce G G Egs 302 CE Se Ee Ee CE VE CE CECT Se CE e CE E C ee C e C ET C Eppendorf Offices AUSTRALIA NEW ZEALAND Eppendorf South Pacific Pty Ltd Tel 61 2 98 89 50 00 Fax 61 2 98 89 51 11 E Mail Info eppendorf com au Internet www eppendorf com au AUSTRIA Eppendorf Austria GmbH Tel 43 0 1 890 13 640 Fax 43 0 1 890 13 64 20 E Mail office eppendorf at Internet www eppendorf at BRAZIL Eppendorf do Brasil Ltda Tel 55 11 3095 9344 Fax 55 11 3095 9340 E Mail eppendorf eppendorf com br Internet www eppendorf com br CANADA Eppendorf Canada Ltd Tel 1 905 826 5525 Fax 1 905 826 5424 E Mail canada eppendorf com Internet www eppendorfna com CHINA Eppendorf China Ltd Tel 86 21 38560500 Fax 86 21 38560555 E Mail market info eppendorf cn Internet www eppendorf cn CZECH
22. t www eppendorf es SWITZERLAND Vaudaux Eppendorf AG Tel 41 61 482 1414 Fax 41 61 482 1419 E Mail vaudaux vaudaux ch Internet www eppendorf ch UNITED KINGDOM Eppendorf UK Limited Tel 44 1223 200 440 Fax 44 1223 200 441 E Mail sales eppendorf co uk Internet www eppendorf co uk USA Eppendorf North America Inc Tel 1 516 334 7500 Fax 1 516 334 7506 E Mail info eppendorf com Internet www eppendorfna com OTHER COUNTRIES Internet www eppendorf com worldwide eppendorf In touch with life 8 5425 901 011 0710909 Printed in Germany Printed on chlorine free bleached paper eppendorf is a registered trademark Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 538 01 0 Fax 49 40 538 01 556 E mail eppendorf eppendorf com Eppendorf North America Inc 102 Motor Parkway Suite 410 Hauppauge NY 11788 5178 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E mail info eppendorf com Application Support Europe International Tel 49 1803 666 789 E mail support eppendorf com North America Tel 1 800 645 3050 ext 2258 E mail support_na eppendorf com Asia Pacific Tel 60 3 8023 6869 E mail support_asiapacific eppendorf com
23. tergent Before doing so disconnect the main power plug Remove the rotor and clean separately The rotor chamber should only be cleaned using a moist cloth Use a mild cleaning agent for cleaning the accessible surfaces of the device Choose disinfection methods that correspond to the current legal regulations and guidelines for your area of application Use for example alcohol ethanol isopropanol or alcohol based disinfectants Please ensure that no liquid enters the slit on the outlet of the motor shaft If condensation water collects in the rotor chamber dry the chamber using a soft absorbent cloth Switch off the centrifuge after use and leave the lid open 4 2 Rotors Rotors must be cleaned at regular intervals in order to prevent that centrifugation residue causes material changes The rotor must be removed to do this In the case of a tightly stuck rotor push the rotor downwards on one side Please check the rotor bores for residue and corrosion The rotor is then inserted again and screwed tight with the supplied rotor key Please check the rotor and rotor bores regularly for corrosion and mechanical damage AN Centrifugation should not be carried out vvith damaged rotors AN 4 3 Aerosol tight rotors the case of the aerosol tight rotor FA 45 24 11 the rotor lid should be replaced vvith signs of vvear Regular care of the sealing ring is necessary to protect the rotors The aerosol tight rotor may not be stored with
24. tion time 100 120 V Deceleration time Centrifuge 5415 R Power supply Power requirement Max rotational speed Max centrifugal force Max load Max kinetic energy Max density of material to be centrifuged 230 V 50 60 Hz 120 V 50 60 Hz see identification plate 180 W 13 200 rpm 16 110 rcf 24 x 2 0 ml Safe Lock Micro Test Tubes or 36 x 0 5 ml tubes 3 100 Nm 1 2 g ml 2 C 35 C 75 Height 230 mm Depth 310 mm Width 230 mm 8 5 kg lt 13 sec lt 11 sec lt 15 sec lt 13 sec 230 V 50 60 Hz 120 V 50 60Hz see identification plate 300 W 13 200 rpm 16 100 rcf 24 x 2 0 ml Safe Lock Micro Test Tubes or 36 x 0 5 ml tubes 3 100 Nm 1 2 g ml 35 0 1 a Technical data 6 Technical data Ambient temperature Max relative humidity Dimensions Weight without rotor Degree of pollution Overvoltage category Acceleration time 230 V Deceleration time Acceleration time 100 120 V Deceleration time Rotors Standard rotor F 45 24 11 Max rotational speed Max centrifugal force Max energy content Special rotor F 45 36 8 Max rotational speed Max centrifugal force Max energy content Technical specifications subject to change 2 C 35 C 75 Height 249 mm Depth 450 mm Width 290 mm 21 2 kg 2 I lt 13 sec lt 11 sec lt 15 sec lt 13 sec 13 200
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