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routines at the department of pharmacy

1. Name type Responsible person person Comments Soe oa FRO Siri Johannesen Siri Johannesen Ultrasentrifuge RISK ANALYSIS Risk Preventive measure Remember to use adapters and balance the tubes before Inbalance sentrifugation HSE INSTALLATIONS e NO 89 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 11 11 2010 BL25 477 DNA RNA LAB Room responsible person Training responsible person ROOM ACTIVITIES e PCR e Realtime PCR Beata Mohebi phone 56554 Beata Mohebi e DNA RNA quantitation e Sentrifugation e RNA isolation e Casting gels e Blood sampling INSTRUMENTS AND EQUIPMENT Name type Responsible person Trainin gresponsiblg Comments person RT PCR ABI PRISM 7000 Beata Mohebi Beata Mohebi SnD ORAY STEN Beata Mohebi Beata Mohebi 9700 Dene ato asp Beata Mohebi Beata Mohebi spektrophotometer Qubit Flurometer Hilde Nilsen Hilde Nilsen Heraeus Fresco 21 Beata Mohebi Beata Mohebi sentrifuge Hettich Universal sentrifuge Hilde Nilsen Hilde Nilsen 2 rotorer Jouan sentrifuge Beata Mohebi Beata Mohebi 2 rotorer Techni Dri Block Beata Mohebi Beata Mohebi RISK ANALYSIS Risk Preventive measure Inbalance in the sentrifuge The tubes must be in balance before sentrifugation Bloodsampling Contagues materials HSE INSTALLATIONS e EYEWASH BOTTLE e FIRE EXTINGUIS
2. Power Supply Biorad Mona Gaarder Mona Gaarder EtBr sensor Vilber Lourmat Mona Gaarder Mona Gaarder Light box RISK ANALYSIS Risk Preventive measure Etbr may cause mutation Wear protective goggles labcoat and gloves INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Eye washer 75 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 164 PROTEIN LABORATORY Room responsible person Mona Gaarder Training responsible person Mona Gaarder ROOM ACTIVITIES e Moulding running and blotting acrylic amide gels e Weighing e Use of microwave oven INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments Scale AND Mona Gaarder Mona Gaarder Scale METTLER Mona Gaarder Mona Gaarder Heating block Mona Gaarder Shaker Mona Gaarder Biorad powerpac 300 Mona Gaarder Mona Gaarder Microwave oven Mona Gaarder Whirlpool RISK ANALYSIS Risk Preventive measure Acrylic amide Wear protective goggles labcoat and gloves Work in the fume hood Buffer Wear nitrile gloves and labcoat Work in the fume hood containing methanol Chemicals Read the safety data sheet before handling chemicals Always wear a lab coat and gloves If necessary also use protective goggles and a face mask INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fume cupboard e Eye washe
3. Name type Responsible person Traing responsible Comments person Autoclave Hoai T N Aas Hoai T N Aas May be used during the naight Risk Preventive measure 42 School of Pharmacy HSE Manual Department of Pharmacy BL25167 BL 25168 Room responsible person Helge Gundersen Training responsible person Helge Gundersen ROOM ACTIVITIES e Laboratory e Lab course preparation room INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible Comments person Cenrtifuge IEC Centra MP4 Tove Larsen Tove Larsen Freezedryer Tove Larsen Tove Larsen Can be used during the night Other instruments Helge Gundersen Helge Gundersen RULES The water supply for the the lab washer and the water still must be turned out during the night Risk Preventive measure Strong acids Protective goggles work in a fume hood Organic solvents Store in a chemical locker and work in a fume hood Labwasher and water still Turn out the water supply at the end of the day 43 School of Pharmacy HSE Manual Department of Pharmacy BL25176 STUDENTS LAB COURSES LABORATORY Room responsible person Tove Larsen ROOM ACTIVITIES e Students lab courses laboratory in medicinal formulation INSTRUMENTS AND EQUIPMENT Name type Responsible person Trame ESLOMSINNE Comments person Freezedryer Tove Larsen Tove Larsen Can be used in the night
4. RISK ANALSIS GENERAL LABORATORY RULES Risk Preventive measure Read Safety Data Sheet in advance Use lab coat and F glows Protective glasses and mouth and nose protection Chemicals if necessary Work in fume hood if necessary Clean up spillage of chemicals immediately Biological material Use lab coat and glows Avoid spillage Gass and gassburner in fume hood Use with caution Ensure that the gas burners work properly They must not burn unnecessary and they shall be closed when leaving the laboratory HSE INSTALLATIONS e EYE WASH BOTTLE 72 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 05 11 2010 BL21 156 CENTRIFUGE ROOM Anne Lise Rishovd Reseach groups and Tor Gjgen Room responsible person Training responsible person ROOM ACTIVITIES e Use of centrifuges INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments Beckman Avanti J 25 High Speed Anne Lise Rishovd Reseach groups Sorval RC 3C Plus Low Speed Anne Lise Rishovd Reseach groups Sorval Discovery 100 Ultrasentrifuge Anne Lise Rishovd Tor Gjgen Sorval Crimper Anne Lise Rishovd Reseach groups RISK ANALYSIS Risk Preventive measure Heavy equipment Careful while carring rotor Do not carry if you have a troublesome back Spill
5. RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Always wear eye protective glasses Consult the material safety data sheets for proper handling of Exposure to harmful chemicals chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders Accidents and injuries due to improper handling of pressurized gas cylinders INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency showe e Eye wash flasks e Chemizorb for collecting spillage of chemicals e Fire blanket e Fire extinguisher CO2 e 7 fume hoods 103 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0192 LABORATORY Room responsible person Training responsible person ACTIVITIES Pal Rongved Pal Rongved e Common laboratory work INSTRUMENTS AND EQIUPMENT Name type Responsible Responsible for training Comments 1 IKA rotavapor Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 2 IKA
6. Use the extinguishing equipment at hand Do not leave the fire as the fire may flare up again On leaving the building or the place of fire close doors and windows but do not lock Explain the incident to the area leader or other person responsible Security and alarm centre 22 85 66 66 University of Oslo Technical department September 2001 Meeting place Between the School of Pharmacy building BL 25 and Helga Engs building BL20 11 School of Pharmacy HSE manual Fire instructions FIRE INSTRUCTIONS FOR USERS The University of Oslo Fire Instructions for Users All users of the University buildings have a responsibility to 12 Conduct their activities in such a way that fire does not easily breakout To reduce the fire hazard it is forbidden to use candles and other forms of open flames in all University buildings with the exception of controlled work in laboratories churches and on staffed catering units where the restaurant has a license to sell alcohol Familiarise themselves with the fire safety measures in the building Take particular note of the location of fire exits fire extinguishing equipment and fire alarms Not damage the fire safety measures that have been implemented This applies in particular to fire exits fire alarm installations emergency lighting fire extinguishing equipment fire walls and fire doors Report anything that is faulty or missing to the person responsible for that section
7. Clean up spillage of chemicals immediately Work benches must be cleaned after use INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e emergency shower just outside the door e fire extinguisher just outside the door e fire blanket just outside the door e 6 fume hoods 110 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 284 WASHING ROOM STORAGE ROOM Room responsible person Iuliana Johansen Training responsible person luliana Johansen ROOM ACTIVITIES e Glassware deposit and withdrawal e Use of the lab washer RULES e The lab washer can be used after reservation or when it is free e Everybody must take away their dishes when it is done INSTRUMENTS AND EQUIPMENT Name type Responsible Responsible for training Comments Industrial washing machine Miele Iuliana Johansen Iuliana Johansen RISK ANALYSIS Risk Preventive measure Exposure to detergents or chemical rests Use personal safety equipment when handling chemicals Clean up spillage of chemicals immediately Work benches must be cleaned after use INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Eye washers e Emergency shower just outside the door e Fire extinguisher just outside the door e Fire blanket just outside the door 111 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0285 A CHEMICALS STORA
8. INSTRUMENTS AND EQUIPMENT Name type Responsible person oe neeponsible Comments BioLC DIONEX Ellen Hanne Cohen PC Printer HP Ellen Hanne Cohen Water purifying apparatus Ellen Hanne Cohen Millipore HPLC VARIAN Helle Wangensteen HOLGER PC Printer HP Ellen Hanne Cohen HPLC LaChrom VWR Helle Wangensteen Rotavapor Suthajini Yogarajah Versa Flash Supelco Helle Wangensteen RISK ANALYSIS Risk Preventive measure Cert eae ee je Read SOP or user s manual before use Always wear protective glasses Exposure to harmful chemicals Always store chemicals in the chemical storage locker Clean up spillage of chemicals immediately INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower in the vicinity e Fire extinguisher e Fire Blanket e Eyewash bottles e Chemical storage locker ventilated e Chemical waste containers 136 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 358 BALANCE ROOM Room responsible person Suthajini Yogarajah Training responsible person ROOM ACTIVITIES e Weighing of samples RULES TO FOLLOW e User must keep order and clean up after themselves e The scale should be cleaned after each use e Turn off lights when leaving room for the day Instruments and equipment Training responsible Name type Responsible person person Comments Analytical scale Sartori
9. Room responsible person Suthajini Yogarajah Training responsible person Supervisor ROOM ACTIVITIES e Preparation for students courses RULES TO FOLLOW e User must keep order and clean up after themselves e Safty cabinets that are not in use shall be closed e The weights should be cleaned after each use e Turn off lights when leaving room for the day INSTRUMENTS AND EQUIPMENT Name type Responsible person a responsible Comments Over Weight Suthajini Yogarajah Pipettes RISK ANALYSIS Risk Preventive measure Use safety data sheets for proper handling and E h hemical i xposure to hazardous chemicals storage of each chemical Personal protective equipment must be used when handling chemicals Chemicals should not be stored outside the chemical cupboard fridge Chemical spills should be removed immediately INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Fire Blanket e First aid kit e Eyewash bottles 135 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 344 LABORATORY Room responsible person Ellen Hanne COhen Training responsible person Supervisor ROOM ACTIVITIES e Research Lab courses e Use of instruments RULES e The users must keep things in order and tidy up e Fume cupboards that are not used shall have their hatches closed e Switch off the light in the end of the day
10. Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e 20 fume hoods e 2emergency showers e 2 first aid kits e 2 fire extinguishers CO 34B e 2 fire extinguisher Glutex ABC powder 89B e 1 fire blanket e A fire hose outside each of the entrances to the room e Eye washers at every bench e Absorbent for spilled chemicals and liquids 109 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0283 ROTAVAPOR ROOM Room responsible person Trond Vidar Hansen Training resposible person Trond Vidar Hansen ROOM ACTIVITIES e Solvent evaporation INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments 6 rotavaporr IKA Biichi Iuliana Johansen Supervisor Tuliana Johansen kapir acM pump Iuliana Johansen Supervisor luliana Johansen VacuuBrand 4 water jet pumps Iuliana Johansen Supervisor luliana Johansen RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Exposure to harmful chemicals kade Gf chemicals Consult the material safety data sheets for proper chemicals Use personal safety equipment when handling
11. ROOM ACTIVITIES Trond Vidar Hansen Trond Vidar Hansen e Practical teaching of general and organic chemistry for lower degree students INSTRUMENTS AND EQUIPMENT f Training Name type Responsible person responsible person Comments 4 scal Atle Haugen Atle Haugen Responsible at the Department of Chemistry scales Elhoussine Merrachi F Atle Haugen Atle Haugen Responsible at the Department of Chemistry 4 heating ovens i Elhoussine Merrachi Atle Haugen Atle Haugen Responsible at the Department of Chemistry 1 UV lamp Elhoussine Merrachi Atle Haugen Atle Haugen Responsible at the Department of Chemistry 3 melting point analyzers Elhoussine Merrachi RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Always wear eye protective glasses Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e 20 fume hoods e 2 emergency showers e 2 first aid kits e 2 fire extinguishers CO 34B e 2 fire
12. Room responsible person Anne Lise Rishovd Training responsible person Reseach groups ROOM ACTIVITIES e Use of analytical instruments INSTRUMENTS AND EQUIPMENT Training responsible person Name type Responsible person Comments ChemiGenius Bio Imaging System Anne Lise Rishovd Reseach groups LightCycler 480 qPCR Anne Lise Rishovd Reseach groups Galaxy S PLUS i n e R h Picodrop Anne Lise Rishovd eseach groups RISK ANALYSIS Risk Preventive measure None in particular GLP HSE INSTALLATIONS e None 79 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 169 ELECTROPHORESIS ROOM Last update 21 06 2010 Room responsible person Training responsible person ROOM ACTIVITIES e Electroforese e Drying of acrylamide gels e Nitrogen flask for AFM work RULES e General laboratory rules INSTRUMENTS AND EQUIPMENT Ewa Jaroszewicz Ewa Jaroszewicz Name type Responsible person Training responsible person Notes Electroforese equipment Ewa Jaroszewicz Ewa Jaroszewicz Gel drying apparatus Ewa Jaroszewicz Research group Ida K Hegna Ida K Hegna Nitrogen flask i Ewa Jaroszewicz Ewa Jaroszewicz RISK ANALYSIS Risk Precaution Exposure to Ethydium bromide acrylamid Read Safety Data Sheets in advance Use saefty equipment EtBr is mutagenic Nitrogen gas under
13. Vita data A S 1991 or confer with the advisor or engineer in the medicinal chemistry group Other hazardous chemical waste that cannot be included under organic solvent waste or pharmaceutical waste must be delivered to the chemicals waste store in the pharmacy building room 009 and sorted into the allocated boxes It is important that chemicals containing heavy metals and reactive chemicals are put into the correct box The boxes must be labelled in accordance with the substance categories in the table in section 5 7 Admission to room 009 is permitted only in company with the engineer in the medicinal chemistry group Appointments with the engineer must be arranged in each case Every six months chemical waste will be declared by the person responsible for chemicals disposal and sent to approved special waste collection centres see section 5 7 Other chemical remnants delivery to waste collection centres 5 2 ORGANIC SOLVENTS WASTE Organic solvents waste that contains less than 10 water must be emptied into the appropriate remnants containers for organic solvents waste Low water content facilitates incineration Rests of organic solvents that contains more than 10 water must be emptied along with halogenated solvents into the right tank in the Workshop yard at the Department of Chemistry HALOGENATED SOLVENTS All halogenated solvents must be emptied into dedicated remnants containers found in each lab
14. We have now decided to combine the monitor and cleaning duty lists for the cell lab Therefore two and two of us work together in doing both tasks in a two week period Make sure that each and everyone notices their cleaning duty weeks If needed you can change places with eachother internally After laboratory work the users must tidy up in the lab and put the dishes in tubs and bowls Drawing ink labels should be removed with technical acetone Any specific dishes are addressed by the users themselves The dishes from the laboratories number 407 409 411 475 477 and 483 are to be put in tubs The person who has the cleaning job that week brings these dishes to room 409 where it is washed in the lab washer When this is done the dishes is placed in drying baskets in room 409 When the dishes are dry their openings are covered with tinfoil and the glassware is sorted and placed in the cupboards in room 409 Pipette tips have to be filled up by the one who empties the box Pipette tips for autoclavation should be placed in a cupboard in room 477 Tips that fits these boxes can be found in the drawers there Old adhesive tape must be removed and a new one is put on Empty glasses for eppendorf tubes that are going to be autoclaved must be filled up You will find the eppendorf tubes in room 477 ALWAYS WEAR GLOVES AND MAKE SURE THE WORKING SURFACE IS CLEAN WHEN YOU FILL UP BOXES WITH TIPS AND GLASSES WITH EPPENDORF TUBES A
15. e Head of Department is responsible for ensuring that the routines are known implemented and complied with e Each department must have a Technical member of staff who is responsible for handling chemicals and maintaining a chemicals register Employees and managers who use the chemicals are obliged to follow the routines 17 School of Pharmacy HSE Manual Common routines 3 PROCEDURES FOR USE AND STORAGE OF CHEMICALS 3 1 GENERAL All chemicals must have a dedicated storage location separate from the workplace when not in use The storage location must be tidy and clearly set out The storage location and chemical containers must be marked with the correct hazard symbol Detailed information on the requirements for the individual chemical s storage conditions and labelling can be found in the chemical HSE datasheet which must be registered in the School s database currently ECOonline Chemicals removed from the chemical stores that are not in use daily must be returned to their dedicated storage location Chemicals that are no longer used and chemicals whose shelf life has expired must be removed preferentially from the storage location and handed in for destruction see section 5 Nevertheless these chemicals can continue to be used for technical tests after assessment for safety and quality by chemists with the appropriate competence 3 2 GENERAL REQUIREMENTS FOR STORAGE ROOMS Storage rooms must have floors walls and ceili
16. Risk Preventive measure Isotopes radiation 3H and 14C Use googels and glows Every user has to attend an aproved course in Radiation protection and radioactivity Special procedure for working with isotopes HSE INSTALLATIONS e Riskwaste e Separat riskwaste for radioactiv waste e Eyewash bottle e Fire extinguisher e Fire blanket 84 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 01 12 2010 BL25 408 EXTRACTION THIN LAYER ROOM Room responsible person Arild Rustan phone 56558 Training responsible person Arild Chr Rustan ROOM ACTIVITIES e Extraction of lipids e Thin layer chromography INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Comments Gas bottle Arild Rustan Arild Rustan Termaxs Incubator Cabinet Arild Rustan Arild Rustan RISK ANALYSIS Risk Preventive measure Isotop radiation hazard All users must complete an approved course Solvents Work in the safety bench use gloves HSE INSTALLATIONS e EYE WASH BOTTLE e FIRE EXTINGUISHER 85 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 03 11 2010 BL25 409 CHEMICAL WEIGHT ROOM Room responsible person Training responsible person ROOM ACTIVITIES e Making solutions e pH adjustment e Chemical regist
17. Risk Preventive measure School of Pharmacy HSE Manual Department of Pharmacy BL25179 BL 25179C Room responsible person Tove Larsen Training responsible person Tove larsen ROOM ACTIVITIES e Production of and characterisation of nano particles INSTRUMENTS AND EQUIPMENT Name type Responsible person Ae FESPONSIDIE Comments person All instruments ToveLarsen Tove Larsen SOP for using the equipment can be found in a binder in the bookshelf in room 180b Reservations for using the equipment should be noted on the whiteboard RULES e All dishes should be rinsed with water or alcohol and then be collected in the blue tub to be washed in the lab washer or kept soaking in the blue tub Water should be replaced when needed baut at least every week e Keep things tidy refill commodities when needed e All stored samples should be labelled e Rotavapor and extruder should not be used after normal working hours Risk Preventive measure Rotovapor and extruder vacuum and pressure SOP and attendance PRESSURIZED GAS See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders 45 School of Pharmacy HSE Manual Department of Pharmacy BL25183 Room responsible person Hoai T N Aas Training responsible person H Aas H H Tgnnesen T Larsen ROOM ACTIVITIES e Instruments laboratory
18. e Balance room INSTRUMENTS AND EQUIPMENT Name type Responsible person POSE Comments person 7 pcs Mettler AE 200 Finn RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY 118 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 214 STUDENTS COURSES LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES Finn T nnesen e Students courses laboratory INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Comments UV Beckman DU 520 Finn UV Beckman Du 530 Finn IR Shimadzu IR 408 Finn Tablets press Finn Heating oven Finn Atomic absorption Ramas spectrophotometer Centrifuge Hettich Finn RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanket e Fire extinguisher 2 pcs e Eye washer 119 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 217 LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES e Lab INSTRUMENTS AND EQUIPMENT Name type Responsible person Pre responsible Comments person RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanke
19. monitors etc must be disposed of in an EE waste container must be ordered after agreement with the local person responsible for equipment The person responsible for IT must be notified when disposing of computer equipment HAZARDOUS WASTE Biological waste contaminated single use items and syringe needles must be packed in hazardous waste boxes cardboard boxes with associated thick plastic bag Sharp objects must be packed well before putting in the plastic bag Syringe needles scalpels can for example be packed in sealed plastic boxes Infected materials of all types small quantities of solvents in sealed containers and gels with toxic substances can be disposed of When the boxes are full they must be properly closed labelled and taken to the Pharmacy building Room 009 In the ZEB building the hazardous waste boxes are taken to the waste room next to the lift in ZEB 2U The hazardous waste boxes will then be sent to be incinerated NB Organic solvents should not be put in the hazardous waste boxes as they can cause uncontrolled incineration 32 School of Pharmacy HSE Manual Common routines WASTE ARISING FROM WORK WITH CELLS CLASSIFIED AS BSL 2 BIOSAFETY LEVEL 2 BSL 2 work in cell laboratories involves heightened safety routines compared to BSL 1 normal cell work The most important requirements relate to signage of areas with restricted access when work is ongoing and at waste treatment autoclaving BSL 2 app
20. CUPBOARDS PURPOSE To establish general basic rules for all work in fume cupboards at the School of Pharmacy The rules must contribute to high levels of safety in the laboratory Everybody must comply with the same basic rules and have the same expectations with regard to how to work in and use fume cupboards so that accidents and injury are prevented RESPONSIBILITY The immediate line manager in each laboratory is responsible for ensuring that the rules for working in fume cupboards are followed The line manager is also responsible for pointing out and following up any breach of the rules Each person who works in one of the School s laboratories is responsible for familiarising themselves with the rules for working in fume cupboards and for complying with them The Head of Department line manager is responsible for ensuring that any necessary protective gear is available PROCEDURE STANDARD WORK ROUTINES FOR WORKING IN FUME CUPBOARDS e Personal protective gear must be used as required e Ensure that the fume cupboard is cleared of unnecessary equipment flasks etc before starting work Flasks and equipment impair the laminar airflow in the cupboard and diminish the extracting efficacy e Check the safety height of the sash Normally this is 25 30 cm When working this height must not be exceeded If however this height is exceeded the volume of air from the cupboard must be increased above normal Some cupboards have automatic
21. FPLC Chromography HTJ HTJ MIS ee AY H reservation necessary Multilabel counter RISK ANALYSIS Risk Preventive measures Solvents Work in safety bench Chemicals Use glasses and gloves HSE INSTALLATIONS e EYEWASH BOTTLE 93 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry ROUTINES AT THE DEPARTMENT OF PHARMACEUTICAL CHEMISTRY HSE Health Safety and the Environment in the workplace Version 2010 Universitety of Oslo School of Pharmacy Department of Pharmaceutical Chemistry 94 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BE23 182B O e O E O A 97 BESA aaa gas vou T A a N eae E oman O a o E T T a a N 98 BE2FOK 19 45 Instr ment 008 AR 99 BL24 K82 Chemicals Storage e nae 100 BL24 O18 Students courses laboratory ars aces eames SAA GAAS SAGAR oss 101 BE24 D188 ROtVapOr TOOM ss 5 6 1 251 24 lala dahlia etl Aa are Eaa raana Ubi ane ule as 102 BL24 0191 Master students laboratorysisicissicsaisasiasivesckeaiievisesterrasscivasastevencsavsbvavssasdseeasandeasaeebonenerans 103 BE24 0192 Laboratoryo isre a RU 104 BE24 196 LC MS a a ETEA e T E EET R 105 BREDA T97 Labor Ar TAAN EAr A rni 106 BLAIR in ee E E E TE 107 BL24G199 Ph D stydents Tabs aaa SE ES E otek 108 BL24 282 Students courses laboratory 5621 65 41 ls bedstalassialesababs lars tavs tate lbshaladsiahessbahslaus ustabsabababaaiohis 109 BE24 283 ROA ADOOS LUIS 110 BL24 284 Wash
22. LCMS LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES e LCMS lab Leon Reubsaet INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person pega Comments LCMS Shimadzu 2010 2stk Leon LCMS Finnigan LCQ Leon Sentrifuge Eppendorf 5804 Leon Vannrenseanlegg Barnstead Finn RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanket e Hazardous waste 115 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 203 CAPILLARY ELECTROPHORESIS LABORATORY Room responsible person Stig Pedersen Bjergaard Training responsible person Stig Pedersen Bjergaard ROOM ACTIVITIES e Capillary electrophoresis e Sample processing RULES e The users must keep things in order and tidy up e Material that has been in contact with biological fluids must be put in the biological waste box e Switch off the light and lock the door when you leave at the end of the day INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Comments CE Agilent 2 pcs eae a Enel al CE Beckman 2 pcs er ar HPLC Agilent a O RISK ANALYSIS Risk Preventive measure Exposure to harmful biological material Refer to the chapters on first aid rules and the common routines for safety and the working environme
23. Responsible Responsible for training Comments 2 Rotavapors Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 2 Scales Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Membrane pump Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 High vacuum pump Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 UV lamp Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Oven Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 4 Magnetic stirrers w temp control Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Heater Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Bunsen burner Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Fridge freezer Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Always wear eye protective glasses Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must b
24. Site code 32 20 12 Central Invoicing Office PO 1074 Blindern N 0316 Oslo The number of kilos litres is filled in either by the person responsible for Chemical Disposal or the transport company that collects the waste Number of containers The number with the same substance no that the Declaration form applies to Mark the boxes with the Declaration number to be found on the top right of the form Description Example Remnants of strong acids from the Chemistry laboratory or Mercury thermometers and other mercury remnants from laboratories or similar descriptions Transport classification Tick the ADR class that applies for road transport ADR classes Class 1 Explosive substances and articles Class 2 Gasses compressed liquefied or dissolved under pressure Class 3 Flammable liquids Class 4 1 Flammable solids Class 4 2 Substances liable to spontaneous combustion Class 4 3 Substances which in contact with water emit flammable gasses Class 5 1 Oxidising substances Class 5 2 Organic peroxides Class 6 1 Toxic substances Class 6 2 Infectious substances Class 7 Radioactive materials Class 8 Corrosive substances Class 9 Miscellaneous dangerous substances and articles TRANSPORT TO WASTE COLLECTION STATION Call Ragn Sells tel 08899 or mail to 08899 ragnsells no 6 REVIEW This routine must be reviewed as required 23 School of Pharmacy HSE Manual Common routines CORRECT USE OF FUME
25. The grinders should be cleaned after each use e Turn off lights when leaving room for the day Instruments and equipment Name type Responsible person ee pespansitle Comments BRABENDER Duisburg Suthajini Yogarajah ALPINE Augsburg Suthajini Yogarajah Freezer RISK ANALYSIS Risk Preventive measure Harmful noise from instruments Wear ear protection Risk of injury from mechanical grinding parts of the mills Be careful while operating the mills Use appropriate protective equipment INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Ear protection BL25 037 STORAGE ROOM Room responsible person Ellen Hanne Cohen Training responsible person Ellen Hanne Cohen Activities e Storage of different kinds of goods 113 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 202 HPLC LABORATORY Room responsible person Leon Reubsaet Training responsible person ROOM ACTIVITIES e HPLC lab INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Comments HPLC Dionex Ultimate Leon Stig HPLC for use in the lab courses Leon RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanket e Eye washer 114 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 203
26. and storing chemicals including in house manufactured mixtures solutions Storage in this instance is the storage of chemicals that are not for immediate use Correct storage of chemicals is necessary in order to reduce the potential for chemical impact on the environment and to reduce the extent of harm if an accident should occur Handling in this instance is the handling of chemicals during use in relation to HSE as well as routines for handling chemicals that are to be destroyed 1 DEFINITIONS Term Explanation Incompatible chemicals Chemicals that react with each other and form Poisonous or explosive compounds gasses or heat For example ox red agents acids bases and combinations of these cyanide salts together with acids Secondary containment Secondary containment Containers vessels that capture spills if the primary container is broken The container must be made from resistant materials Flashpoint The temperature at which a fluid in a given flashpoint device at a certain air pressure produces so much vapour that at ignition a flame occurs above the surface of the liquid Fire cell Entire building or sections of a building in which a fire can freely develop without spreading to other buildings or other parts of the building within a given time Class A liquid Flashpoint lt 23 C Class B liquid Flashpoint between 23 C 55 C Class C liquid Flashpoint above 55 C 2 RESPONSIBILITY
27. autoclavation Pack the equipment for autoclavation and place it by the autoclave Run autoclavation when there is enough to fill up an autoclave Pack equipment for dry sterilization and put it in the heating oven that is used for drysterilization Start sterilization when there is a reasonable amount of equipment Put yellow and blue tips Eppendorf tubes and kits for autoclavation If you have any spare time please bring filled risk waste containers down and clear away glassware and clutter that has been forgotten Perhaps you can clean up around for instance the electrophoresis equipment or other things One of the engineers will accompany everyone the first week as a monitor to ensure that everyone are trained in using the autoclave the dry sterilization unit and the lab washers 53 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 2U27 FREEZER ROOM Last update 21 06 2010 Room responsible person Ewa Jaroszewicz Training responsible person Ewa Jaroszewicz Anne Lise Rishovd ROOM ACTIVITIES e Storage of bacteria collection e Storage og reagents RULES e General laboratory rules INSTRUMENTS AND EQUIPMENT Name type Responsible person a responsible Notes Freezer 20 C Ewa Jaroszewicz Ewa Jaroszewicz Freezer 80 C Ewa Jaroszewicz Ewa Jaroszewicz Freezer 80 C Anne Lise Rishovd Anne Lise Rishovd RISK ANALYSIS Risk Precaution Storage of spore fo
28. by prolonged exposure through inhalation in contact with skin and if Very toxic to aquatic organisms may cause long term adverse effects in the aquatic environment Toxic to aquatic organisms may cause long term adverse effects in the aquatic environment Harmful to aquatic organisms may cause long term adverse effects in the aquatic environment Harmful possible risk of irreversible effects through inhalation Harmful possible risk of irreversible effects in contact with skin Harmful possible risk of irreversible effects if swallowed Harmful possible risk of irreversible effects through inhalation and in contact with skin Harmful possible risk of irreversible effects through inhalation and if swallowed Harmful possible risk of irreversible effects in contact with skin and if swallowed Harmful possible risk of irreversible effects through inhalation in contact with skin and if swallowed School of Pharmacy HSE Manual Appendices SAFETY PHRASES At hazard marking the following phrases must be used to describe necessary preventive measures for safe handling and use of the chemicals S1 S2 S3 S4 S5 S6 S7 S8 S9 s12 S13 S14 S15 S16 S17 S18 S20 S21 S22 S23 S24 S25 S26 S27 S28 S29 S30 S33 S35 S36 S37 S38 S39 S40 distributor S41 S42 S43 S45 S46 S47 S48 S49 S50 S51 S52 S53 S56 S57 S59 S60 S61 S62 S63 S64 S210 Keep locked up Keep locked up and out of the reach of childre
29. dangerous bromine Br is reduced by the thiosulphate S203 to the relatively harmless bromide Br ETHIDIUM BROMIDE This chemical crystals and solutions is a potent mutagen e If inhaled or swallowed seek medical advice e Ifin contact with the eyes or skin wash for many minutes with water e Inthe event of spillage wash with 1 2 Deconex PHENOL C H50H ON SKIN e Rinse with water Next wash the skin repeatedly with glycerol or aqueous polyethylene glycol PEG Apply pyrisept ointment liberally to the skin MERCURY Hg SPILLAGE ON WORKBENCHES FLOORS e Draw up the metallic mercury using an eye dropper into a container Spread sulphur powder S over the contaminated area Hg S HgS Leave for a short while before collecting everything together in a container Mercury absorbent can be used instead of sulphur S The container is to be disposed of as chemical waste Heavy metals mercury ACCIDENTS WHEN WORKING WITH RADIOACTIVE SUBSTANCES All employees who work with radioactive substances in the department must complete Kurs i stralevern og radioaktivitet Course in radiation prevention and radioactivity Department of Chemistry UiO before start of the work Other than this there is daily instruction in work routines in the laboratory through personal instruction e g higher degree students PRACTICAL MEASURES IN THE EVENT OF AN ACCIDENT In the event of an accident with radioactive substances me
30. emergency calls as appropriate to Fire Services tel 110 Police tel 112 Oslo A amp E services tel 113 UiO Health Services 22 85 31 74 Ulleval University Hospital A amp E Reception tel 22 11 73 50 Ulleval University Hospital Department of Ophthalmology tel 22 11 85 45 22 11 85 47 The Norwegian Poison Information Centre tel 22 59 13 00 Taxi emergency help tel 22 38 80 50 3 Immediately notify the accident by phone Head of Department or their deputy to The Safety Delegate The Director School of Pharmacy tel 22 85 6586 who forwards the information to the Faculty Administration Section for HSE at UiO tel 22 85 88 95 The Norwegian Labour Inspection Authority 2nd District tel 23 08 05 05 fax 22 17 78 10 4 Secure the site of the accident if possible until the police Norwegian Labour Inspection Authority arrives 14 School of Pharmacy HSE Manual Common routines POST INCIDENT ACTIONS IN THE EVENT OF ACCIDENTS NEAR ACCIDENTS 5 All accidents near accidents must be recorded on internal accident notification forms and sent to the Director School of Pharmacy with a copy to the HSE Section at UiO no later than 3 days after the accident The Director School of Pharmacy must archive a copy in the injured person s employee folder The internal accident notification form minus personal details is sent to the Head Safety Delegate at UiO In the event of serious accidents or as r
31. extinguisher Glutex ABC powder 89B e 1 fire blanket e A water fire hose outside each of the entrances to the room e Eye washers at every bench e Absorbent for spilled chemicals and liquids 101 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0188 ROTAVAPOR ROOM Room responsible person Training resposible person Trond Vidar Hansen Trond Vidar Hansen ROOM ACTIVITIES e Solvent evaporation INSTRUMENTS AND EQUIPMENT Name type Responsible Training Comm nts person responsible person Responsible at the Department of Chemistry 4 rotavapors IKA Atle Haugen Atle Haugen i Elhoussine Merrachi i Ri ible at the Department of Chemistry 2 aphia eni pompa Atle Haugen Atle Haugen fea Sica SE a ieee VacuuBrand Elhoussine Merrachi RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Clean up spillage of chemicals immediately Work benches must be cleaned after use INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e emergency shower in the neighbouring room 187 e fire extinguisher CO just outside the door e fire extinguisher Glutex ABC powder just outside the door
32. magnetic stirrers Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 2 water jet pumps Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 AGA pressureized argon cylinder 200 bar 3000 psi Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Thermaks heating oven Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Ohaus laboratory scale Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Always wear eye protective glasses Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use Accidents and injuries due to improper handling of pressurized gas cylinders See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower 5 meters to the right outside the door e Fire extinguisher e Fire bl
33. manual before use Always wear eye protective glasses Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use Accidents and injuries due to improper handling of pressurized gas cylinders See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower to the right outside the door e Eye wash flasks e First aid kit e Fire extinguisher CO2 and fire blanket e 2 fume hoods 98 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 OK19A INSTRUMENT ROOM Room responsible person luliana Johansen Atle Haugen Training responsible person luliana Johansen Atle Haugen ROOM ACTIVITIES e Deposit and withdrawal of organic solvents and NMR solvents e Use of instruments e Solvent drying RULES Deposit withdrawal of the organic and NMR solvents of most use is exempted from the normal chemicals registration procedures Each laboratory is registrated with a certain constant amount of the organic and NMR solvents of mos
34. must not be stored in attics or basements due to the seasonal variation of the outside temperature combined with a lack of inside temperature control 3 6 FIREPROOF CABINETS FRIDGES IN LABORATORIES In laboratories flammable substances must be stored in dedicated fireproof cabinets The cabinets must have a fire resistance of at least 20 minutes The cabinets must be ventilated and when scrapping old cabinets these must be replaced by ventilated cabinets The doors must always be closed when not in use Fridges that are used to store flammable substances must be specially designed and constructed for this purpose Fireproof cabinets and fridges which are used to store flammable substances must be marked with the correct hazard signs see Appendix in the HSE manual A laboratory with a closed door constitutes a fire cell Up to 40 litres of Class A liquids may be stored here 18 School of Pharmacy HSE Manual Common routines 3 7 SUBSTANCES WITH SEVERAL HAZARD CLASSES Some chemicals come under several hazard classes In such cases the hazard classes are ranked as follows with the greatest hazard listed first 1 Flammable substance 2 Oxidising substance 3 Acids 4 Bases 5 Miscellaneous Chemicals that are both flammable and oxidising must for example always be stored in a fireproof cabinet 3 8 DRY CHEMICALS LIQUIDS When not in use chemicals must be stored in the designated storage location Chemicals that are stored in
35. person Training responsible Comments person 1 Nuire incubator 4500 E Camilla Stensrud Engineer CO2 1 Nuire incubator 4750 E Camilla Stensrud Engineer CO2 1 Forma Scientific Camilla Stensrud Engineer incubator CO2 2 Laf benches Holten Camilla Stensrud Engineer Safe 2000 Sentrifuge Kubota 2010 Camilla Stensrud Engineer Microscope Leica DMIL Camilla Stensrud Engineer Water bath Camilla Stensrud Engineer Refrigerator and freezer Camilla Stensrud Engineer 18K 19 K RISK ANALYSIS Risk Preventive measure Biological material may be contagious Use glows a special coat and shoes Working with virus Special procedyre Gas cylinder The cylinder is secured Gas flame Use carefully Filling the N2 freezer Heavy lifting HSE INSTALLATIONS e Riskwaste e Eyewash bottle e Fire blanket 92 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 08 11 2010 BL25 484 MICROPLATE READER AND PROTEIN CHROMOGRAPHY Room responsible person Harald Thidemann Johansen phone 56595 Training responsible person Harald Thidemann Johansen ROOM ACTIVITIES e For quantitative detection of light emitting and light absorbing marker e Detection of protein and enzymes e Protein chromatography INSTRUMENTS AND EQUIPMENT Name type Responsible person Traning Tesponpibie Comments person
36. pressure Nitrogen flask must be properly secured INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e No 80 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 170 P 2 ROOM Last update 01 11 2010 Room responsible person Training responsible person ROOM ACTIVITIES e Growing of spore forming bacteria e Electroporation e OD measure RULES e General laboratory rules Ewa Jaroszewicz Ewa Jaroszewicz e Procedures for handling of spore forming bacteria INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Notes LAF bench Holten Lamin Ewa Jaroszewicz Air Ewa Jaroszewicz Spectrofotometer Eppendorf Ewa Jaroszewicz Ewa Jaroszewicz Various eqipment Ewa Jaroszewicz Ewa Jaroszewicz Equipment for electroporation from BioRad Macia Mona Gaarder 2 CO incubators Ewa Jaroszewicz Ewa Jaroszewicz 2 Incubators Ewa Jaroszewicz Ewa Jaroszewicz RISK ANALYSIS Risk Precaution Exposure to spore forming bacteria Use safety equipment Work in LAF bench Clean up workplace before and after with 70 EtOH Liquid bacteria waste must be autoclaved INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e No 81 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences CLEANING PROCEDURES IN THE THIRD FLOOR
37. regulation of the air volume constant flow e Choose your working position sitting or standing based on the risk of liquid splash and the duration of the work Never bend your head in the cupboard but re assess the work method or the test set up e Position the work volatile liquids materials that release dust etc as far as possible in the centre of the cupboard Generally suction is strongest here Work using calm movements in the cupboard This stops turbulence and movement of polluted air into the breathing zone TIDINESS AND CLEANLINESS IN FUME CUPBOARDS e Tidy and clean after working The fume cupboard must not be used for storage Inflammable corrosive irritating liquids chemicals and similar must be put back in the chemicals cupboard with ventilation CONDUCT WHEN HANDLING CHEMICALS e Do not expose others or yourself to unnecessary health hazards Use the fume cupboards Transport of chemicals between the chemicals cupboard and the fume cupboards must take place with minimum risk to yourself and others in the laboratory e At tests that involve the use of hazardous chemicals a copy of the HSE datasheet must be easily accessible in the laboratory e At tests that involve the use of hazardous chemicals the work procedure must be designed to take this into account so that necessary technical checks of process equipment and safety equipment are conducted in advance It must be ensured and documented that the equipment and ma
38. shower to the right outside the door e Eye wash flasks e Fire extinguisher CO2 e Fire blanket e 2 fume hoods e Air conditioning system 105 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0197 LABORATORY Room responsible person Trond Vidar Hansen Training responsible person Trond Vidar Hansen ROOM ACTIVITIES e Handling of chemicals e Organic synthesis e Preparative chromatography e Use of instruments rotavapor vacuum pumps e Use of gas under pressure nitrogen and argon INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments 1 Rotavapor Atle Haugen Tuliana Johannesen Atle Haugen Tuliana Johansen 1 Scale Atle Haugen Tuliana Johannesen Atle Haugen Tuliana Johansen 1 Vacuum pump Atle Haugen Tuliana Johannesen Atle Haugen Tuliana Johansen 1 Oven Atle Haugen Tuliana Johannesen Atle Haugen Tuliana Johansen 1 UV lamp Atle Haugen Tuliana Johannesen Atle Haugen Tuliana Johansen oe an wW ate Haug n Taliana Johan es n Atle Haugen Tuliana Johansen 1 Fridge freezer Atle Haugen Tuliana Johannesen Atle Haugen Tuliana Johansen RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Always wear eye protective glasses Exposure to harmful chemicals Consult the material safety data sheets for proper handlin
39. the first week of employment This must be documented in the same way as above This also applies to temporary employees temps and consultants who it is anticipated will work on that floor for more than 1 week Routinely checking the emergency escape routes and other technical fire measures in their area of responsibility If any faults or deficits are found these must be reported to the Fire Safety Officer with a copy for the Head of Department Forms for these reports can be found in the fire safety documentation and on the Technical department s webpage Electronic mail may also be used Knowing the content of the fire safety documentation and where it is located Informing the Head of Department of people with disabilities on their floor in their department Cooperating with the Head of Department Fire Safety Officer and Safety Delegate to ensure the satisfactory safety of people with disabilities in the event of a fire Familiarising at least one deputy with these instructions In the event of a fire alarm Put on the fire safety vest and encourage people on your floor in your department to leave the building Leave the building and ensure that people do not stand right outside the entrance Notify the person in charge at the Fire Services of any people who have not left the building and where they may be found Inform users on your floor in your department of the cause of the fire alarm These tasks may be delegated but the overall
40. to change disposable gloves frequently SWALLOWING CHEMICALS e Drink plenty of water dilution effect Get medical help Call the Norwegian Poison Information Centre Giftinformasjonen tel 22 59 13 00 e If organic solvents hydrocarbons etc are swallowed give fats such as cooking oil milk cream or ice cream e If organic solvents or corrosive liquids are swallowed the person must not vomit as the fluids can enter the lungs and result in chemical pneumonia NB Do not give food or drink if the person is unconscious e If NaOH sodium hydroxide is swallowed give milk if possible e Emetic 2 dessert spoons of table salt NaCl sodium chloride in a lukewarm glass of water INHALATION OF POISONOUS GAS VAPOUR OR DUST e Fresh air and complete rest Contact the doctor ambulance If respiration stops give artificial resuscitation mouth to mouth WOUNDS FROM GLASS OR SIMILAR e Wash the wound thoroughly with cold water to remove any possible contamination and to cool the skin so that the blood vessels contract Try to stop reduce the bleeding by applying a plaster sterile bandage If the wound is large or relatively deep contact A amp E to check whether stitches are necessary BURNS e Cool immediately and continuously in cold water for at least 20 mins and continue during transport to the doctor ELECTRIC SHOCK e Strong blow to the chest if the heart has stopped If necessary immediately commence heart massage an
41. type Responsible person Training responsible person Comments All instruments Solveig Kristensen Risk Preventive measure Rotovapor Acids and solvents Pressureized gas cylinders Work in a fume hood and always wear protective gear See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders 47 School of Pharmacy HSE Manual Department of Pharmacy BL25188 Room responsible person Hoai T N Aas Training responsible person H Aas H H Tgnnesen ROOM ACTIVITIES e Instruments laboratory with gas INSTRUMENTS AND EQUIPMENT Name type Responsible person pene responsive Comments UV and HPLC H Aas H Aas Fluorometer S Kristensen S Kristensen Lifetime spectrophotometer H H T nnesen H H T nnesen Risk Preventive measure Pressureized gas See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders 48 School of Pharmacy HSE Manual Department of Pharmacy BL25224 Room responsible person Training responsible person ROOM ACTIVITIES Hoai T N Aas Jan Karlsen e Instruments laboratory with gas Risk Preventive measure Pressureized gas See section The laboratory Safety and the working environment in this handbook to ensure
42. use hazards and general safety at use of a rotavapor ULTRASOUND BATH Shakes molecules loose Do not put body parts into the vessel UV LAMPS HAND HELD LAMPS WITH AND WITHOUT STAND AND IN INSTRUMENTS Short wavelength light 254nm and long wavelength light 365 366nm UV light must never be directed at people The wavelength and energy are inversely proportional This means that the shorter the wavelength the greater the energy and the greater the danger 28 School of Pharmacy HSE Manual Common routines HOT AIR PISTOL ELECTRIC High temperature strong heat Exercise caution when using Turn off when not in use Hot air pistols can cleave products ignite solvents and cause fires and burns SCALES Must be kept clean to avoid exposure to chemicals or biological materials TOXIC SUBSTANCES AND HAZARDS All users are responsible for checking the safety datasheets prior to using the chemical substance See the School s Substances and Chemicals Register that describes the hazards and preventive measures www ecoonline no DECONTAMINATION OF BIOLOGICAL WASTE AND EQUIPMENT MAIN WASTE GROUPS A Disposable equipment such as pipettes pipette tips Eppendorf tubes syringes and used microscope slides B Solid biological waste e g used agar dishes and cell cultivation equipment C Suspensions of cells bacteria or virus and the equipment these have been in contact with e g cultivation flasks glass pipettes test tub
43. 22856585 instituttkontoret farmasi uio no z Reception Blindern VIP 31494 http www farmasi uio no gt gt a Technical Dept Lower 22 857293 teknisk nedre admin uio no 5 N Blindern http www admin uio no ta E gt Operations office E Department of Chemistry 22 855469 vidar blekastadOkjemi uio no m 8 Head of Safety 913371 73 5 VIP 31154 E Department of Chemistry 22 855446 ekspedisjonen kjemi uio no Reception http www kjemi uio no Section for HSE 22858895 hms post admin uio no at UiO http www uio no om organisasjon ad m opa hms Labour Inspection 23080505 http www arbeidstilsynet no
44. 32 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 339 STUDENTS COURSES LABORATORY Room responsible person Suthajini Yogarajah Training responsible person Supervisor ROOM ACTIVITIES e Students courses e Use of instruments RULES TO FOLLOW e User must keep order and clean up after themselves e Safty cabinets that are not in use shall be closed e The weights should be cleaned after each use e Turn off lights when leaving room for the day INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Comments Over weight Suthajini Yogarajah Analysis weight Suthajini Yogarajah UV photo meter Centrifuge Suthajini Yogarajah pH meter Suthajini Yogarajah Rotary evaporator one f Suthajini Yogarajah organic solution Water bath Suthajini Yogarajah Chemical Cabinets w exhaust Suthajini Yogarajah RISK ANALYSIS Risk Preventive measure Incorrect use of instruments and equipment Read SOP or manual before use Exposure to hazardous chemicals Use safety data sheets for proper handling and storage of each chemical handling chemicals Personal protective equipment must be used when Chemical spills should be removed immediately Chemicals should not be stored outside the chemical cupboard fridge INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower
45. ASSES Acetylene Maroon Carbon dioxide Grey Oxygen White Helium Brown Argon Dark green Hydrogen Red Nitrogen Black Nitrous oxide Blue CYLINDER COLOURS AGA GAS SUPPLIER Industrial gasses Black Acetylene Maroon Food industry gasses Green Special gasses Silver grey Medical gasses White 142 School of Pharmacy HSE Manual Appendices RISK PHRASES At hazard marking of dangerous chemicals the following statements R phrases must be used to describe the hazards R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 R11 R12 R14 R15 R16 R17 R18 R19 R20 R21 R22 R23 R24 R25 R26 R27 R28 R29 R30 R31 R32 R33 R34 R35 R36 R37 R38 R39 R40 R41 R42 R43 R44 R45 R46 R48 R49 R50 R51 R52 R53 R54 R55 R56 R57 R58 R59 R60 R61 R62 R63 R64 R65 R66 R67 R68 Explosive when dry Risk of explosion by shock friction fire or other sources of ignition Extreme risk of explosion by shock friction fire or other sources of ignition Forms very sensitive explosive metallic compounds Heating may cause an explosion Explosive with or without contact with air May cause fire Contact with combustible material may cause fire Explosive when mixed with combustible material Flammable Highly flammable Extremely flammable Reacts violently with water Contact with water liberates extremely flammable gases Explosive when mixed with oxidising substances Spontaneously flammable in air In use may form fla
46. ER WASTE Paper and cardboard Paper waste must be disposed of in the paper waste container by the user Containers for waste paper are located at Pharmacy building platforms in the main stairs on each floor every Thursday at 09 13 Physics building by the east lift on each floor on Fridays at 09 13 Chemistry building by the east lift on each floor on Thursdays at 09 13 ZEB building by the lift on the 3rd floor every Thursday at 09 13 GLASS WASTE Cleaned glass waste broken glass and TLC plates The following are not glass waste Used silica chemical remnants plastic corks and fluorescent light bulbs Used silica must be handed in as chemical waste and contaminated glass must be packed in well and disposed of in the hazardous waste boxes Glass waste must be taken by the user to the glass waste containers located at Pharmacy building Room 025 and the workshop yard in the Chemistry building ZEB building Glass waste must be put in glass waste boxes and placed in the waste room located by the side of the lift in ZEB 2U HAZARDOUS WASTE Waste that cannot be disposed of safely together with consumer waste because it could cause serious pollution or risk of harm to people animals birds and fish Chemical waste is described on page 20 EE WASTE ELECTRIC AND ELECTRONIC WASTE Waste such as white goods cables and leads computer equipment office machines measuring instruments medical equipment circuit cards
47. GE ROOM IR ROOM Room responsible person Iuliana Johansen Training responsible person Iuliana Johansen ROOM ACTIVITIES e Deposit and withdrawal of chemicals and glassware for the lab courses FRM 1000 1010 1030 e Use of the IR instrument INSTRUMENTS AND EQUIPMENT Name type Responsible person Tranne pele Comments person IR instrument Perkin Elmer Iuliana Johansen Iuliana Johansen No hood short of space PC printer connected to the IR instrument Iuliana Johansen Iuliana Johansen 1 scale Iuliana Johansen Iuliana Johansen RISK ANALYSIS Risk Preventive measure Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Always store chemicals in the chemical storage locker Work benches must be cleaned after use Clean up spillage of chemicals immediately INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Eye washer e Emergency shower just outside the door e Fire extinguisher just outside the door e Fire blanket just outside the door 112 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 018 MILL ROOM Room responsible person Suthajini Yogarajah Training responsible person ROOM ACTIVITIES e grind raw materials RULES TO FOLLOW e User must keep order and clean up after themselves e Use appropriate protective equipment e
48. HER 90 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 02 11 2010 BL25 480 ISOTOPIC LABORATORY Arild Chr Rustan phone 56558 Arild Chr Rustan Room responsible person Training responsible person ROOM ACTIVITIES e Work with radioactive sources e Storage of radioactove isotopes e Laboratory is approved as a type C laboratory Anyone working with radioactive isotopes shall be completed before start working with radiation and radioactivity INSTRUMENTS AND EQUIPMENT Training responsible culture microscope STC BI Name type Responsible person person Comments Sentrifuge Sigma 2 15X Arild Rustan Arild Rustan Incubator cabinet Jouan i IGO 150W gas sylinder Arild Rustan Arild Rustan metic puc Te Arild Rustan Arild Rustan RISK ANALYSIS Risk Preventive measure Isotopes All users must complete an approved course Gas under pressure HSE INSTALLATIONS e EYEWASHBOTTLE e FIRE EXTINGUISHER e FIRE BLANKET e GEIGER MULLER COUNTER 91 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL25 481 CELL LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES Camilla Stensrud Engineer or Camilla Stensrud e The major activity is cellgrowth INSTRUMENTS AND EQUIPMENT Name type Responsible
49. HSE Health Safety and the Environment SCHOOL OF PHARMACY HSE MANUAL 07 04 2011 School of Pharmacy HSE manual Contents CONTENTS INTRODUCTION 0 3 FIRST AID RULES at A A seso iese siae 8 FIRE INSTRUCTIONS yeas cities 52 tae cat ceca casicecauicachceciascenidec decascandesunaciceceatdonses ae eecssanusauaesheaatecadicacesbesesasunees 11 COMMON ROUTINES 2 14 ROUTINES AT THE ADMINISTRATION ide 35 ROUTINES AT THE DEPARTMENT OF PHARMACY ccccsssssssssssssscsssssssssssssesssssssssssessssessseeees 37 ROUTINES AT THE DEPARTMENT OF PHARMACEUTICAL BIOSCIENCEG ccscssssssssseees 50 ROUTINES AT THE DEPARTMENT OF PHARMACEUTICAL CHEMISTRY sccccsssssesssseeees 94 APPENDICE Sa 140 LIST OF TELEPHONE NUMBERS AND ADDRESSES sccsscsssssssssssesssscsesssessssssssssssssssssssesesees 148 School of Pharmacy HSE manual Introduction INTRODUCTION PREFACE The University in Oslo and the Faculty of Mathematics and Natural Sciences HSE Manuals apply to the School of Pharmacy The HSE Manual for the School of Pharmacy therefore covers special regulations that apply exclusively to the School as well as information that is useful for HSE work here The Manual contains an organisation chart displaying the responsibility and decision taking structure in HSE work at the School Conditions relating to Local Working Environment Committees are described and references are given to general laws and regulat
50. Heeponsible Notes Autoclave large Thune Mona Gaarder Mona Gaarder Autoclave small HMC Mona Gaarder Mona Gaarder 2 lab washers Miele Mona Gaarder Mona Gaarder MQ og RO water purifying equipment Mona Gaarder Mona Gaarder Millipore RISK ANALYSIS Risk Preventive measure Pathogenic bacteria Wear a labcoat and gloves Avoid any spillage INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Eye washer 68 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 151 CELL CULTIVATION LABORATORY Room responsible person Mona Gaarder Training responsible person ISA group Anne Lise Rishovd Tor Gjgen Neuro group Mona Gaarder Ragnhild Paulsen ROOM ACTITIVIES e Cultivation of virus from fish e Cultivation of cell lines e Preparation of primary cultures INSTRUMENTS AND EQUIPMENT Name type Responsible person a di Hote person s Termo Forma cupboard with Hepafilter and Mona Gaarder og Ragnhild M Gaarder og CO Paulsen R Paulsen Forma Scientic cupboard with CO Incubator for fish 15 og 20 degrees centigrade Anne Lise Rishovd og A L Rishovd og T Termaks Tor Gj en Gj en Water bath Grant Mona Gaarder M Gaarder Mona Gaarder Anne Lise M Gaarder A L Rishovd LAF Bench x 2 Holten Safe 2000 Rishovd Nikon TMS Microscope Mona Gaarder M Gaarder RISK ANALYSIS Risk Preventive measure Pressureized The cylinders are secu
51. LLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e No 62 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 1U53 COOLING ROOM Room responsible person Mona Gaarder tlf 44945 28 ROOM ACTIVITIES Storing material for lab course use RULES Keep things in order 63 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 1U62 LAB COURSE PREPARATION ROOM Room responsible person Training responsible person ROOM AVTIVITIES e Lab course preparation Cultivation of bacteria and fungi RULES e General laboratory rules INSTRUMENTS AND EQUIPMENT Mona Gaarder Mona Gaarder Name type Responsible person Training responsible person Notes PFGE Instrument Bio Rad CHEF DR III system Beckman GeneLine Ewa Jaroszewicz Ewa Jaroszewicz RISK ANALYSIS Risk Preventive measure Pathogenic bacteria Use a labcoat and gloves Avoid any spillage Fungi Use a labcoat and gloves and a face mask Gas jet Be careful when you use it INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Eye washer e First aid kit 64 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 1U64 POLAROID CAMERA ROOM NOT NAMED BY THE ELEVATOR Room responsible person Ole Andreas L chen kstad Training responsible person Ole Andreas L chen kstad ROOM AC
52. Lab course preparation ro in ea eieo ra aN raara anaE ESEESE EAT ERa EE ESEE EEEa 64 BL21 1U64 Polaroid camera room not named by the elevator eee ceeeecceeeeeceeeeesnneeeeeceeeeeeetneeeeeetees 65 B21 1U65 Weighing room o eh iis EEEE EAEE AEE EE eel wees 66 BE21 1U67 Medium culti vation TOMA Aah en A Ae E 67 BL21 1U69 Autoclave cleaning rOOM a i ieten tenti ee iadi e ea a eai ara iae a e aeaa 68 BL21 151 Cell cultvation laboratory e e e iio nnn EEE ea nannies 69 j o BAAI oE Cold TOM AAAA T AAA AAA AAAA AAAA AAAA 70 BL21 153 Micros op TOOM enen cesescdeneenepecehebevscehepeesiducusvisebebeveebsdeveebsoesduceevecdesbenebeyeonsbeoseets 71 BL21 155 Research laboratory imc ida iii aa 72 BL21 156 Centrifuge TOM ia ad dies 73 BE21 163A Film developing r60M ici Gehan Anan tee a en nae ee eee 74 BEZ21 163B Electrophoresis laboratory c 60h WR A ER a 75 BL21 164 Protein laboratory coccion ee RNR AM neta ee nel eee lc 76 BLZ1 166 Research laDOLatOrY ecotocanotacotitatacecida dodo aaa citado ci leledacidadi ci a adi cctalodolebadanid i e a a dot cera ta cido 77 BL2T 167 Research laboratory A AN 78 BL21 168 Instrument TOOM irern en rial levee ce decest edie aiii iii Bates 79 BL21 169 Electrophoresis Tom cole e E AEE EEEN S EREE CEEE Ea e iesea a 80 BEZ2L 170 PLATO A A A E EE Baa RA Hoe 81 Cleaning procedures in the third floor octe ten eto a a et ae aE a E ai ana 82 BL25 310 Nitrogen tankan cold Storage nonn e aa aeaa a
53. MENT AND SECURITY 123 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 229 GC GCMS LABORATORY Room responsible person Finn Tgnnesen Training responsible person ROOM ACTIVITIES e GC GCMS lab INSTRUMENTS AND EQUIPMENT Name type Responsible person Tratomg responsible Comments person GC Fison 8000 series Finn GC Shimadzu 2010 Finn GC Varian Star 3400CX Finn GCMS Fison MD800 Finn GCMS Shimadzu 2010 Stig Finn RISK ANALYSIS Risk Preventive measure Leakage of combustible gases from gas cylinders Risk of Refer to the Laboratory Safety and the working explosion at a fire environment chapter for proper use and handling of pressureized gas cylinders INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanket e Fire extinguisher e Eye washer 124 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 314 COOLING ROOM Room responsible person Suthajini Yogarajah Training responsible person Supervisor ROOM ACTIVITIES e Storage of the gel filtration columns e Kit and special substances e freezing in liquid nitrogen RULES TO FOLLOW e User must keep order and clean up after themselves e Chemical spills should be removed immediately e Turn off lights when leaving room for the day INSTRUMENTS AND EQUIPMENT Name type Responsible person Traning responsib
54. Research e Use of instruments RULES e The users must keep things in order and tidy e Clean up spillage of chemicals immediately e Switch off the lights in the end of the day INSTRUMENTS AND EQUIPMENT Name type Responsible person A responsible Comments person Fraction collector Ellen Hanne Cohen Pipettes Ellen Hanne Cohen Heating oven Room responsible person Ultra sonic bath Room responsible person Rotavapor Suthajini Yogarajah RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and k Read SOP or user s manual before use equipment INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Fire blanket e Eye washer e Chemical waste containers 130 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 334 INSTRUMENTLAB Room responsible person Berit Smestad Paulsen Training responsible person Supervisor ROOM ACTIVITIES e Research lab courses e Use of instruments RULES e The users must keep things in order and tidy up e The users must remove paper and glass waste when it s needed e Fume cupboards that are not used shall have their hatches closed e The lab scales must be cleaned after use e Switch off the light in the end of the day INSTRUMENTS AND EQUIPMENT Name type Responsible person o responsible Comments GC
55. Shimadzu Ellen H Cohen GC Carlo Erba Ellen H Cohen PC Laser printer Ellen H Cohen HPLC Shimadzu Ellen H Cohen Radioactive detector HPLC RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of E Read SOP or user s manual before use instruments and equipment Always wear protective glasses Exposure to harmful chemicals Always store chemicals in the chemical storage locker Clean up spillage of chemicals immediately Leakage of combustible gases from gas cylinders Refer to the Laboratory Safety and the working environment Risk of explosion at a fire chapter for proper use and handling of pressureized gas cylinders INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Fire blanket e Eye washer 131 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 336 DRUG COLLECTION Room responsible person Permanent scientific employees at the department Training responsible person Chief of lab courses supervisors ROOM ACTIVITIES e Student s discussion groups e Drug exhibition RULES e Always keep the room tidy INSTRUMENTS AND EQUIPMENT Name type Responsible person seen Fesponpinte Comments Blackboard Large table RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Fire blanket 1
56. TERIALS AND CULTURES When working with biological materials or cultures it is important to wash your hands frequently in order to avoid infection Use hand cream as well Solutions that contain live materials virus or plasmids must be autoclaved prior to disposing of the solution down the sink Bottles and flasks with contents must be labelled The label must correspond to the content Remove the label before the equipment is sent for washing Do not use other people s pipettes without asking CHEMICALS Chemicals must be treated with caution because most of them are poisonous and several of them react with air water alcohols etc Read the safety datasheet prior to opening the chemical container and prior to use The date for receipt of the chemical container from the supplier is to be noted if not already noted on the container Substantial changes in the chemical stocks must be notified to the person responsible for the Substances and Chemicals Register Dangerous chemicals that are not in use must be stored in the chemicals cupboard with ventilation This also applies to remnant cans containing organic solvent remnants The only exception is concentrated hydrochloric acid HCl which can be placed in the fume cupboard to prevent corrosion of the chemicals cupboards All chemical containers must be marked and a list of codes that explain the contents must be easily accessible Chemical spills must be removed immediately Remnant cans m
57. TIVITIES e Photographing documentary proof of results at visible light e Photographing documentary proof of results at UV light RULES e Tidy up e Clean the light box with alcohol when you are finished e Do not use the equipment until you are fully trained INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Notes Polaroid camera and UV light box Ole Andreas L chen Vkstad RISK ANALYSIS Risk Preventive measure Do not expose your skin or eyes to UV light Wear a face shield gloves and a lab coat INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e None 65 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 1U65 WEIGHING ROOM Last update 21 06 2010 Room responsible person Training responsible person ROOM ACTIVITIES e Storage of chemicals e Making of solutions and media e pH measure e Storage of Safety Data Sheets RULES e General laboratory rules INSTRUMENTS AND EQUIPMENT Ewa Jaroszewicz Ewa Jaroszewicz Name type Responsible person Training responsible person Notes pH meter 744 Metroholm Ewa Jaroszewicz Ewa Jaroszewicz Weights Ewa Jaroszewicz Ewa Jaroszewicz Heating plates Ewa Jaroszewicz Ewa Jaroszewicz RISK ANALYSIS Risk Precaution Exposure to harmful chemicals Read Safety Data Sheets in advance Use safet
58. Y TOXIC ETSENDE OXIDISING EXTREMELY FLAMMABLE HIGHLY FLAMMABLE CORROSIVE TOXIC DANGEROUS FOR THE ENVIRONMENT HARMFUL IRRITANT The hazard symbols must be printed in black on an orange background The hazard designation must be denoted together with the hazard symbol The hazard code that is above the symbols e g F Xi is not to be included on the hazard label HAZARD CLASSES BASED ON RISK PHRASES Hazard class 3 and Risk phrases Hazard Hazard symbol Hazard 6 code designation Explosive R2 R3 E Explosive Explosive Oxidising R7 R8 R9 O Oxidising Oxidising Extremely flammable R12 F Flame symbol Extremely flammable Highly flammable R11 R15 R17 F Flame symbol Highly flammable Flammable R10 None None None Very toxic R26 R27 R28 R39 T Skull and Very toxic crossbones Toxic R23 R24 R25 R39 R48 T Skull and Toxic crossbones Harmful R20 R21 R22 R68 R48 R65 Xn X cross Harmful Corrosive R34 R35 C Corrosive Corrosive pictogram Irritant R36 R37 R38 R41 Xi X cross Irritant Sensitiser R42 Xn X cross Harmful 140 School of Pharmacy HSE Manual Appendices R43 Xi X cross Irritant Carcinogenic R45 R49 T Skull and Toxic crossbones R40 Xn X cross Harmful Mutagenic R46 T Skull and Toxic crossbones R68 Xn X cross Harmful Harmful to R60 R61 T Skull and Toxic reproduction crossbones R62 R63 Xn X cross Har
59. aaea aaa alliance east 83 BL25 407 Isotope laboratory a a a a ar aaa aaa a aT a a aa T A a aa aa naa aia ara a a ea a dro ce ent hati 84 BL25 408 Extraction thin layer r00M oooonnonccccnnoocnnnnonanonnnononannnnnnnnnnonnnnnnnonnn ono ono rnnn ocre ron anne rnnn nac rnnn ano nncnnnnnss 85 BL25 409 Chemical weight room iis 2 ccc cedecececetsee ita A ida tata 86 BESA HP rooms ieee iy seca Aaa o ees 87 BL25 413E Western electrophoresis lab cuina olaa 88 BL25 413J outside Sorval Ultra Centrifuge ooooccncnnnnononoccnonnnancnnnannnnnnncnnonnnnnnnncnnnnnnn ceeeeesaaaeeeceeeeeeeeeaeeeees 89 BSAF DNA RNA Tab stocisst e tins suits nie ee ees ee aia Hetil 90 BL25 480s Isotopic laDOrdtOL Y isictootericotatoricadicadiosn dente idolo a a a a a dad Ta a a ena a aan a Aaa arara a a na annie 91 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL25 481 Cell laboratory ooonoooocccnnnccnnnocccnncnnaccnnnnnnnns BL25 484 Microplate reader and protein chromography 52 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 CLEANING ROUTINES IN THE ZEB BUILDING Responsible person Mona Gaarder tlf 44928 45 RULES Bring the wash down each morning or when the baskets are filled enough Do the washing in the lab washer Put the wash in the electric drying cabinet After drying distribute to the cabinets autoclavation and dry sterilization Bring down floating bacterial waste from all groups for
60. achines should not be unattended while in use e Use a hood and a labcoat e Face shield protective goggles and ear protection Rests from the production is classified as hazardous waste e Evacuate the air from the units under pressure before disconnection Risk Preventive measure High power processing equipment pressureized air Coat hood ear protection face shield dust noise 39 School of Pharmacy HSE Manual Department of Pharmacy BL25011 Room responsible person Helge Gundersen Training responsible person Helge Gundersen ROOM ACTIVITIES e Fluid bed for coating granulating RULES e Protective goggles e Use ear protection very noisy room Risk Preventive measure Pressureized air dust noise Ear protection face shield 40 School of Pharmacy HSE Manual Department of Pharmacy BL25012 Room responsible person Training responsible person ROOM ACTIVITIES Helge Gundersen Helge Gundersen e Temperature controlled cupboard and air compressor RULES e Protective goggles e Use ear protection very noisy room Risk Preventive measure Pressureized air dust noise Ear protection face shield 41 School of Pharmacy HSE Manual Department of Pharmacy BL25029 Room responsible person Hoai T N Aas Training responsible person Hoai T N Aas ROOM ACTIVITIES e Laboratory for sterile production INSTRUMENTS AND EQUIPMENT
61. age of bacterial culture Avoid spillage Handling spillage with disinfectant thoroughly and clean all surfaces HSE INSTALLATIONS e None 73 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 05 11 2010 BL21 163A FILM DEVELOPING ROOM Room responsible person Anne Lise Rishovd Training responsible person Anne Lise Rishovd ROOM ACTIVITIES e Develop films e Use of UV box INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person Per n Comments AGFA Curix 60 Anne Lise Rishovd Anne Lise Rishovd UV Translink 20 S Anne Lise Rishovd Reseach groups RISK ANALYSIS Risk Preventive measure Chemical solutions gloves Use safety glasses handling chemicals and rubber me cutting gels Use safety glasses and gloves no bare skin while HSE INSTALLATIONS e Protective plexiglas mask 74 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 163B ELECTROPHORESIS LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES Mona Gaarder Mona Gaarder e DNA separation using normal electrophoresis INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments Easy Cast Elektrophoresis system Mona Gaarder Mona Gaarder Pharmacia Elektrophoresis equipment Mona Gaarder Mona Gaarder
62. aia 131 BE25 336 Drug collection inn ana e eii a E e EEEE e P a E E Oe aT ERE n AE 132 BL25 339 Students courses labora iirmearnincrnia ana nn nnn never woes dr 133 BL25 339 B Chromatography labs ic cece ee iglesia aiii 134 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 340 Preparation TOOI COUESES 00 A A E a eiae 135 Joi BAE Bl O thle 136 BL25 338 Balance TOM AA 137 BL23 30237 LADO A dd estad aten ao Dio ds 138 BLEZ5 363 Conference 100 Ml inona e an seta ca evita ansias Giiyestaectesshs sodas arneses 139 96 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL23 V182B LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES e Handling of chemicals e Organic synthesis e Preparative chromatography Jo Klaveness Jo Klaveness e Use of instruments rotavapor vacuum pumps e Use of gas under pressure nitrogen and argon INSTRUMENTS AND EQUIPMENT Name type Responsible Responsible for training Comments 2 Rotavapors Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 2 Scales Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Membrane pump Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 High vacuum pump Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 UV lamp Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Oven Atle H
63. andling of nitrogen 70 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 153 MICROSCOPY ROOM Room responsible person Karen A Boldingh Debernard Training responsible person Each research group ROOM ACTIVITIES e Microscopy RULES e You are not allowed to use the instruments without any training e Do not fix anything if you do not know what it is e See the instructions at the microscopy room for lighting and extinction of UV lamps cleaning of the lenses and so on e As for confocal see separate instruction explaining how to switch the instruments on and off INSTRUMENTS AND EQUIPMENT Name type Responsible person Training do Comments person Nikon Eclipse TE300 microscope with Ragnhild Paulsen Each research group Intensilight C HGFI Tor Gjgen Training course Nikon Elipse TE 2000 E microscope Ragnhild Paulsen or similar type of with D Eclipse C1 confocal Tor Gjgen experience is 3 lasers necessary before you are allowed INU Tokai hit touse th TMC air table confocal Gas cylinders microscope Ida Hegna Ewa Labophot 2 fluorescence mikroskop Jaroszewicz RISK ANALYSIS Risk Preventive measure As a general rule there is a low risk of being injured when the equipment in this room is used as it is meant to be The confocal microscope uses lasers Authorized users only are allowed to use the instrument Never look in class 3B into the l
64. anket e PLUM eye washer e First aid kit e Gas mask e 1 fume hood 104 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0196 LC MS LABORATORY Room responsible person P l Rongved Training responsible person P l Rongved ROOM ACTIVITIES e Chemical compound analysis by high pressure liquid chromatography mass spectrometry e Student teaching and instruction INSTRUMENTS AND EQUIPMENT Name type Responsible person Trine responsible Comments person High pressure liquid chromatography mass Iuliana Johansen Atle Haugen Tuliana Johansen spectrometry Agilent Nitrogen generator Agilent Iuliana Johansen Atle Haugen Tuliana Johansen Air compressor Jun Air Iuliana Johansen Atle Haugen Tuliana Johansen Vacuum pump Edwards luliana Johansen Atle Haugen Tuliana Johansen Samp need elin DOT luliana Johansen Atle Haugen Tuliana Johansen cylinder RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of F Read SOP or user s manual before use instruments and equipment Never conduct any experiments involving open fumes of harmful chemicals neither in the fume hoods or in the room itself Exposure to harmful chemicals due to insufficient ventilation Use protective hearing gear during lasting work in this Health injury due to noise room INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency
65. ansen Atle Haugen 2 heating cabinets Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen 50 L Nitrogen pressure tank Iuliana Johansen Atle Haugen luliana Johansen Atle Haugen 50 L Argon pressure tank luliana Johansen Atle Haugen luliana Johansen Atle Haugen RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Always wear eye protective glasses Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use Accidents and injuries due to improper handling of pressurized gas cylinders See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower outside the door e Fire extinguisher e Fire blanket e 3 fume hoods e Chemical storage cabinet e Refridgerator 108 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0282 STUDENTS COURSES LABORATORY Room respons
66. armasi Avdelingsleder Avdelingsleder Avdelingsleder Director of the School of Pharmacy Administration Studies Finances IT Department of Department of Department of Pharmacy Pharmaceutical Pharmaceutical Chemistry Biosciences Head of Department Head of Department Head of Department LOCAL WORKING ENVIRONMENT COMMITTEE L AMU SIZE AND COMPOSITION Employer representatives 2 Director School of Pharmacy and one employer representative elected by management School of Pharmacy HSE manual Introduction Employee representatives 2 Head Safety Delegate and one employee representative elected by the employees Employer representative for student matters 1 Secretary of L AMU Student representative 1 One student elected by the Pharmacy Subject Committee WORK TASKS L AMU is responsible for updating the School s HSE Manual School of Pharmacy HSE manual Introduction HSE MANUALS The HSE Manuals for UiO the Faculty of Mathematics and Natural Sciences and the School of Pharmacy apply to everyone on the premises of the School of Pharmacy A declaration confirming familiarity with the HSE Manuals and signed by the employee must be archived in the Department A declaration signed by students undertaking a laboratory based master thesis is to be archived with the student advisor responsible for the 2nd department HSE TRAINING The HSE course run by the Faculty of Mathematics and Natural sciences is co
67. aser beam Do not detach anything Cool the lasers after use Mercury lamps are used for UV Never look into the UV light Refer to the separate instruction on light replacing lamps working hours etc In the case of an accident pull out the plug leave the room ventilate thoroughly for at least 30 minutes before tidying Pressureized gas cylinders The cylinders are securely tightened to the wall 71 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 155 RESEARCH LABORATORY Room responsible person Hanne C Winther Larsen Training responsible person Hanne C Winther Larsen Anne Lise Rishovd ROOM ACTIVITIES e Research activitites e RNA work e Use of instruments INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments Mini centrifuge 7M Hanne Winther Larsen He ae Sanit bale Ane Lise Rishovd Mini centrifuge Galaxy Panne Winer Tarsi Hanne C Winther Larsen Anne ministar Lise Rishovd Incubator Innova 4230 Hanne Winther Larsen Ha mec Winther LarsenAnne Lise Rishovd Fume hood Hanne Winther Larsen Ha mee Winther Larsen Anne Lise Rishovd Gel electrophoreses for Ewa Jaroszewicz Ewa Jaroszewicz RNA in fume hood Research groups Bosch 20 C freezer Mona Gaarder Mona Gaarder Combi fridge freezer Hanne Winther Larsen ma nne C nine Tasca Ae Lise Rishovd Simens 20 C freezer Anne Lise Rishovd Anne Lise Rishovd
68. asures must be initiated promptly to limit spread and unnecessary exposure The contaminated area must be cordoned off and access must be blocked Protective gear must be used gloves coats disposable shoecovers and measuring devices If equipment and or workplace have been contaminated the decontamination procedure must be performed Washing must be carried out for example with 5 Deconex preferably several times If it is not possible to remove the contamination in this way the HSE department must be notified A contaminated surface that cannot be decontaminated must be removed or screened if necessary Contaminated clothes must be changed and School of Pharmacy HSE manual First aid rules washed separately Contaminated skin must be washed immediately with soap and water All accidents with radioactive substances must be notified immediately to the responsible persons in the department Safety delegate Radiation Safety Coordinator and Head of Department 10 School of Pharmacy HSE manual Fire instructions FIRE INSTRUCTIONS Alert Assist Extinguish Control damage Guide Fire alarm 110 Police 112 Ambulance 113 General rules in case of fire If you discover fire or you hear a firealarm you are obliged to leave the building Depending on circumstances you may Everybody in the area should be alerted Use fire alarms when provided Call 110 Help people who need assistance to the nearest safe place
69. augen Tuliana Johansen Atle Haugen Tuliana Johansen 4 Magnetic stirrers w temp control Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Heater Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Bunsen burner Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Fridge freezer Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Always wear eye protective glasses Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use Accidents and injuries due to improper handling of pressurized gas cylinders See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower to the left outside the door e Eye wash flasks e First aid kit e Fire extinguisher CO2 and fire blanket e fume hood 97 S
70. ays wear protective glasses Exposure to harmful chemicals Always store chemicals in the chemical storage locker Clean up spillage of chemicals immediately INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Fire Blanket e Eyewash bottles e Chemical waste containers 138 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 365 CONFERENCE ROOM Room responsible person Ellen Hanne Cohen Training responsible person ROOM ACTIVITIES e Conference room e Common room for the Department e Library room for the Department RULES e The users must keep things in order and tidy up e Switch off the light in the end of the day INSTRUMENTS AND EQUIPMENT Name type Responsible person eae responsible Comments Common PC Ellen H Cohen Overhead projector Ellen H Cohen Wall screen Ellen H Cohen RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e The room nearby 139 School of Pharmacy HSE Manual Appendices APPENDICES PRODUCT LABELLING OF CHEMICALS HAZARD SYMBOLS WITH HAZARD DESIGNATION The following hazard codes hazard symbols and hazard designation apply at labelling of chemicals C E O F F EKSTREMT MEGET EKSPLOSIV OKSIDERENDE BRANNFARLIG BRANNFARLIG T T N Xn Xi MEGETGIFTIG GIFTIG MILJOSKADELIG HELSESKADELIG IRRITERENDE VER
71. centrifuge tubes must be open Only centrifuge tubes in PP plastic can be autoclaved Soft agar tubes and corex tubes can be placed in autoclaving trays INACTIVATION PROCEDURES METHODS FOR CHEMICAL DISINFECTION 1 SODIUM HYDROXIDE PEARLS Inactivation of cultures e g E coli 2 pearls 100ml 2 5 CHLORAMINE Disinfection at spills on benches 3 VIRKON Disinfection of equipment that cannot be autoclaved Removal of any spills on benches 4 DECONEX Primarily for difficult cleaning but has disinfectant properties 5 RECTIFIED ALCOHOL 70 Disinfection of equipment that cannot be autoclaved Removal of any spills on benches 31 School of Pharmacy HSE Manual Common routines WASTE HANDLING The user is responsible for ensuring that all waste they generate is destroyed and or taken to the nearest collection point See in addition Routines for handling and storing chemicals at the School of Pharmacy Rutine for h ndtering og lagring av kjemikalier ved Farmas ytisk institutt under Common routines NORMAL WASTE REMNANT WASTE Only non hazardous waste is to be handled without gloves Plastic waste food waste dirty paper and similar Normal waste must not contain sharp objects etc that could injure cleaning staff Larger quantities of plastic and or polystyrene are to be taken to the outside waste remnants containers by the user The remaining normal waste is collected by the cleaning staff PAP
72. chool of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL23 0184 LABORATORY Room responsible person Training responsible person Jo Klaveness Jo Klaveness ROOM ACTIVITIES e Handling of chemicals e Organic synthesis e Preparative chromatography e Use of instruments rotavapor vacuum pumps e Use of gas under pressure nitrogen and argon INSTRUMENTS AND EQUIPMENT Name type Responsible Responsible for training Comments 2 Rotavapors Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 2 Scales Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Membrane pump Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 High vacuum pump Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 UV lamp Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Oven Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 4 Magnetic stirrers w temp control Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Heater Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Bunsen burner Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen 1 Fridge freezer Atle Haugen Tuliana Johansen Atle Haugen Tuliana Johansen RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s
73. correct handling of pressurized gas cylinders 49 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences ROUTINES AT THE DEPARTMENT OF PHARMACEUTICAL BIOSCIENCES HSE Health Safety and the Environment in the workplace Version 2010 Universitety of Oslo School of Pharmacy Department of Pharmaceutical Biosciences School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences TABLE OF CONTENTS BL21 Cleaning routines in the ZEB building onr aer eetet e EE A erea pere cono n nro E cnc cnn nanannnass 53 BL21 2U27 Freezer TOO Micenas cello E E ronca miis 54 BL21 2U53 ISotope foom clasg Bihan dee ANA A a eee A einen 55 BL21 2U54 Freezing room 80 centigrade cooooocccnnonncccnnonnncnnnnconnnocnnnnnnnnnnnnnnonnanonnn anar nnno ronca acc cnannanannnnss 56 BL21 2U63A Storage TOO Minsa a lead as rd aie An da Da bad 57 BL21 2U63B Atomic force microscopy TO0M ccccoooocccnnononnnnnonnnononnnoonnnnnnnnnnncrnnnnnnennnn nen nan teisia eeir eiia 58 BL21 2U67 Freezer cold TO Mii eee ene ae 59 BL21 2U68 Molecular dynamics storm 860 errore eeii riers rr e ue e e Pr E E E E EE S Eiee 60 BE2V VS Course TOM o A I ada a a e a tod a ed 61 BL21 1U53 Centrifuge bacteria growing TO0M cooooocccnnnononcnnnonncnnnnnnnonccnnnnnnnnnnnnnnnnnnnn nec nnn ceaaeeceesaaeeceessaeeeesaas 62 BEZI TUSZ COON a TOOM aa a a ar a a T a a a a Ta a a aaa E a en aana conti debate ta a ea boleto ld 63 BL21 1U62
74. d artificial respiration mouth to mouth resuscitation FAINTING e Ensure the airways are free place the person in a comfortable position stable side position loosen any tight clothes and check the pulse and breathing As necessary Cardiopulmonary resuscitation with heart massage and artificial respiration mouth to mouth resuscitation SHOCK e Try to calm the injured person The injured person should preferably lie or sit comfortably with their feet above the level of their head Make sure they have fresh air and are comfortably warm School of Pharmacy HSE manual First aid rules ACCIDENTS INVOLVING BIOLOGICAL MATERIALS Spillage of cultures or other biological material Spills must be disinfected immediately Using gloves remove the spillage using paper wetted with 70 alcohol Wet the dried surface with 70 alcohol and leave to stand for a few minutes Wipe dry Dispose of the paper and gloves in a hazard waste box ACCIDENTS WITH CHEMICALS SOME SPECIFIC In general refer to the safety data sheets in the Substances and Chemicals Register ECOonline See the dedicated section on Safety datasheets and the Substances and Chemicals Register under The Laboratory Safety and the Working Environment BROMINE Br ON SKIN e Rinse the skin with plenty of sodium thiosulphate solution Na25205 Afterwards rinse with 60 ethanol and polyethylene glycol Or use pyrisept ointment with polyethylene glycol as the ointment base The
75. e 56538 Training responsible person Siri Johannesen ROOM ACTIVITIES e Freezing of cells INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments Locator JR Cryo Biological Storage Systems Xxxx Siri Johannesen Thermo Liquidnitrogen Siri Johannesen monitor RISK ANALYSIS Risk Preventive measure Tubes may explode when taken out of the cryo Use mask and mittens when you fetch out the cells HSE INSTALLATIONS e NO 83 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 12 11 2010 BL25 407 ISOTOPE LABORATORY 1 Room responsible person Camilla Stensrud Training responsible person Arild Rustan ROOM ACTIVITIES e Radioactive isotopes are measured in a scintilation countermachine e Sonication e Use of utrationbath INSTRUMENTS AND EQUIPMENT Name type Responsible person Traning TEPO IDI Comments person Scintillation counter Arild Rustan Arild Rustan 1450 Microbeta plus Scintillation Betacounter Arild Rustan Arild Rustan model TRI CARB 1900 TR Ultrasonic Processor Engineer Engineer Ultrabad Sonorex AK Engineer Engineer 100 Ultrabad Bransonic 220 Engineer Engineer Termax serie 4000 Engineer Engineer Laserprinter Hilde Nilsen Engineer Refridigator freezer K8 F9ogF 10 RISK ANALYSIS
76. e cleaned after use Accidents and injuries due to improper handling of pressurized gas cylinders See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower to the left outside the door e Eye wash flasks e First aid kit e Fire extinguisher CO2 e 3 fume hoods 107 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 199 PH D STUDENTS LAB Last template update 11 05 2010 Room responsible person Training responsible person ROOM ACTIVITIES Trond Vidar Hansen Trond Vidar Hansen e Synthetic organic chemistry INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments 2 rotary evaporators Iuliana Johansen Atle Haugen luliana Johansen Atle Haugen 1 membrane pump VacuuBrand Iuliana Johansen Atle Haugen luliana Johansen Atle Haugen 2 water jet pumps luliana Johansen Atle Haugen Iuliana Johansen Atle Haugen 4 magnetic stirrer plates Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen UV lamp Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen Refrigerator with chemicals Iuliana Johansen Atle Haugen luliana Johansen Atle Haugen Laboratory scale Iuliana Johansen Atle Haugen luliana Joh
77. e person Ida K Hegna Ewa Jaroszewicz ROOM ACTIVITIES e Research e Use of instrument in nanotechnology RULES e General laboratory rules INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Notes Atomic For ce Microscop Ida K Hegna Ida K Hegna Nano Wizard from JPK with microskop from Nicon Ewa Jaroszewicz Ewa Jaroszewicz Active vibration isolation Ida K Hegna l a K Hegna system MOD 1 i HALEYONICS Ewa Jaroszewicz Ewa Jaroszewicz RISK ANALYSIS Risk Precaution No INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e No 58 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 2U67 FREEZER COLD ROOM Last update 21 06 2010 Room responsible person Ewa Jaroszewicz Training responsible person Ewa Jaroszewicz ROOM ACTIVITIES e Storage of various reagents at 4 C and 20 C RULES e General laboratory rules INSTRUMENTS AND EQUIPMENT Name type Responsible person A responsible Notes person No RISK ANALYSIS Risk Precaution INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e No 59 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 2U68 MOLECULAR DYNAMICS STORM 860 Room responsible person Tor Gj en tlf 44943 ROOM ACTIVITIES Phosphor Imager cassette reading and blot scanning chemolu
78. e water fire hose just outside the door e 6 fume hoods e first aid kit 102 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0191 MASTER STUDENTS LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES Trond Vidar Hansen Trond Vidar Hansen e Teaching and supervision of master students e Practical organic synthesis as part of master theses e Sample purification using liquid chromatography INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments 2 rotavapors Iuliana Johansen Atle Haugen luliana Johansen Atle Haugen Liquid chromatograph Flash Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen 3 scales Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen 3 heating ovens Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen 2 UV lamps luliana Johansen Atle Haugen luliana Johansen Atle Haugen 4 magnetic stirrers with heating Iuliana Johansen Atle Haugen luliana Johansen Atle Haugen 2 vacuumpumps Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen 2 cylinders of pressurized N2 50 L Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen 1 heating mantle Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen 1 fridge freezer Iuliana Johansen Atle Haugen Iuliana Johansen Atle Haugen
79. eer RISK ANALYSIS Risk Precaution Work with bacteria and fungi Use safety equipment lab coat Gloves and googgles are used when work requires that Clean up spillage of bacteria or fungi with 70 EtOH Exposure to harmful chemicals Use safety equipment Work in the fume hood Read Safety Data Sheets in advance Work with an open gas flame Students must familiarize themselves with placement of fire equipment fire alarms emergency exits emergency showers INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower e Fire equipment e Fire blanket e Eye wash bottles 61 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 1U53 CENTRIFUGE BACTERIA GROWING ROOM Last update 21 06 2010 Room responsible person Training responsible person ROOM ACTIVITIES e Centrifugation e Growing of bacteria Ewa Jaroszewicz Ewa Jaroszewicz INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Notes Centrifuge Beckman Ewa Jaroszewicz Ewa Jaroszewicz Table incubator New Brunswick Scientific Ewa Jaroszewicz Ewa Jaroszewicz RISK ANALYSIS Risk Precaution Exposure to spore forming bacteria and fungi Use safety equipment Clean up spillage of bacteria with 70 EtOH All bacterial waste must be autoclaved INSTA
80. eless happen TIDINESS Everybody is responsible for keeping their laboratory tidy and for clearing up after themselves in other rooms The laboratories must be kept clean and tidy at all times Contact the person responsible for the room if necessary The laboratories must be locked and windows closed when they are vacated to prevent access by unauthorised persons Normal laboratory working hours are from 8 00 16 00 The name of the person responsible for the room must be posted at the entrance to all special rooms The person responsible for the room has a particular responsibility for following up the activities in the room and for ensuring that users tidy up after themselves It is not their responsibility to tidy up after others Chemicals equipment must not be lent out removed from the place of use without the permission of the person responsible for the room or for the instruments The person responsible for the room or for the instruments must be notified of any defective equipment Each laboratory must have the School s HSE Manual relevant sections and folder with HSE datasheets visible and easily accessible chemicals cupboards with ventilation as required for storage of dangerous chemicals and fume cupboards as required for working with these chemicals Breach of HSE regulations the UiO HSE Manual the Faculty of Mathematics and Natural Sciences HSE Manual the School of Pharmacy HSE Manual and the Working Environment Ha
81. equested supplementary information on the course of the incident must also be sent to the Director School of Pharmacy the HSE Section at UiO and the Norwegian Labour Inspection Authority 2nd District The Director School of Pharmacy reports an injury to the injured person s Social Security Office RTV Form IA 13 07 05 when indicated by the injury and always when the injured person has received medical treatment the injury results in incapacity the injured person wishes this the Social Security Office requests this The notification must be sent as soon as possible and no later than 3 days after the incident Remember to report all injuries Seemingly minor injuries can develop into serious problems with major economic consequences The injured person must contact their local Social Security Office Pension Fund for reimbursements Reimbursement is conditional on notification of the injury to the appropriate body Consent must be obtained from the injured person to enable use of the personal details from the accident in internal accident prevention efforts The Working Environment Act 20 If consent is not given the form must be stored confidentially in the injured person s company medical records 15 School of Pharmacy HSE Manual Common routines REGISTERING ACCIDENTS AND NEAR ACCIDENTS PURPOSE To secure the rights of the injured person s when an undesired incident has occurred To prevent new accidents and inj
82. es and centrifuge tubes with lids DISPOSAL OF WASTE TYPES A AND B Such waste is to be placed in specially marked cartons for hazardous waste The cartoons must be lined with correspondingly marked waterproof yellow black plastic sacks NB Do not overfill the cartons as they must be closed without compressing the contents When the boxes are full they are to be brought down to the waste disposal room next to the lift in the 2nd lower floor Ragn Sells will take them from here to be incinerated DISPOSAL OF WASTE TYPE C There are two ways of treating the solution before it is disposed of down the sink Hazard class I 1 Autoclave 2 Chemical destruction Addition of sodium hydroxide pearls leave for 30 mins Example MAXI PREP Culture remnants autoclaved Supernatant Tipped back into the culture flask and autoclaved Centrifuge tubes Inactivated with alcohol MINI PREP SMALL VOLUME IN SEALED TUBE IN HAZARDOUS WASTE LDL E 29 School of Pharmacy HSE Manual Common routines Hazard class II 2 and sporogenous bacteria Mandatory autoclaving 30 School of Pharmacy HSE Manual Common routines AUTOCLAVING PROCEDURES Bacteria and cell cultures that are to be autoclaved are placed in the washroom in metal trays marked area Test tubes are placed in separate suitable racks in empty autoclaving trays Centrifuge tubes are filled with water and put in the same place To avoid deformation the
83. eventive measure Chemicals Use googles gloves and back bench HSE INSTALLATIONS e FIRE EXTINGUISHER e FIRE BLANKET e EYEWASH BOTTLE 87 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 02 11 2010 BL25 413E WESTERN ELECTROPHORESIS LAB Room responsible person Hilde Nilsen tlf 56547 Training responsible person Hilde Nilsen and Siri Johannesen ROOM ACTIVITIES e Electropheresis e Casting protein gels e Casting agarose gels e Developing INSTRUMENTS AND EQUIPMENT Name type Responsible person tere Teponsihle Comments Electropheresis equipment Hilde and Siri Hilde and Siri Hoefer gel washer Hilde and Siri Hilde and Siri Spectrophotometer Hilde and Siri Hilde and Siri Polaroid gel Kamera Beata Mohebi RISK ANALYSIS Risk Preventive measure Acrylamide Cast gels in safety bench use gloves Mercaptoethanol Pippette in safety bench use gloves Eth bromide Pippette in safety bench use gloves HSE INSTALLATIONS e EYE WASH BOTTLE 88 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 11 11 2010 BL25 413J OUTSIDE SORVAL ULTRA CENTRIFUGE Room responsible person Training responsible person ROOM ACTIVITIES e Sentrifugation Siri Johannesen phone 56554 Siri Johannesen INSTRUMENTS AND EQUIPMENT Training responsible
84. f Pharmacy HSE Manual Common routines The shed with the waste tanks 5 3 PHARMACEUTICAL WASTE Pharmaceuticals in small quantities may be handed into the chemicals waste stores in the Pharmacy building Room 009 the key can be obtained from the office in Reception Larger quantities must be packed in hazard waste boxes and placed in Room 009 in the Pharmacy building 5 4 COMBUSTIBLE PAPER WASTE Paper towels and similar that could self ignite must be rendered safe before discarding The paper must be placed in a fume cupboard to evaporate off Waste containers for flammable waste can be provided red metal containers that the users empty 5 5 GLASS WASTE Glass waste is collected together and brought to special containers in the Workshop yard NB Chemical bottles must be completely empty and cleaned before disposal NB Very important Only clean glass is to be put in this container No ceramics plastics metals paper or fluorescent light bulbs 5 6 SYRINGE NEEDLES CONTAINING SOLVENT REMNANTS ETC These are placed in special dispenser boxes can be obtained from the Central Stores at the Faculty of Chemistry or empty chemical jars If the syringe needles do not contain hazardous materials the boxes can be screwed tightly and disposed of with normal waste Otherwise they are disposed of in the Hazard waste boxes that are then taken to the Pharmacy building Room 009 They are collected from here by a company that takes them directl
85. f Pharmacy HSE Manual Department of Pharmacy TABLE OF CONTENTS Installations regarding health security and envirOnMentt eeesecceescecceesceseeeeeessaeeecesssaeecssaeecescessaeeeeeeeaes 39 BL25010B Tablet rooms aii e EE dorada eliana Ees 39 BEZSO M TE E EEE E EE EA 40 BL2Z30 NAAA AAAA AAAA AAE AAAA AAAA A AAN AAAA AAAA AAAS 41 A EENE TAS 42 BL25167 BL 25168 ui at A A A RR AA RR A A AE Ad ee 43 BL25176 Students lab courses laboratory oooooocccnnonocccnonacnnnnononannonononnnncnonnnnnnnnn ano cnc nana cnc nan nana cnannncrnnnncnns 44 BE2S1790BLZLSTIOC cidad AAA Ai Ae 45 BLSE ae at el e E a e io 46 BLZILSO rito tilo E A soloorbr onto crint toto dario EE E coto t Ario toi 47 BICQ5 1B Bracers tatesisetatate tebelenetatade seta ta bottle tates cal toco A AN AAAA atleta denia do 48 Bla 220A nek ea ee EE A ARRAN Ga ee eA Rea 49 38 School of Pharmacy HSE Manual Department of Pharmacy INSTALLATIONS REGARDING HEALTH SECURITY AND ENVIRONMENT e Emergency shower in the womens lavatory in the ground floor room 172 e Eye washer e Fire blanket e First aid kits in room 010B 029 167 176 224 BL25010B TABLET ROOM Room responsible person Helge Gundersen Training responsible person Helge Gundersen ROOM ACTIVITIES e Rooms containing larger pharmaceutical processing equipment e Tablets pellets spraydrying e Itis not allowed to use the instruments after normal working hours e Tablet producing m
86. floor Report all damage accidents and near accidents that have or could have led to fire or outbreak of fire This must be reported on the standard form on the web pages of the Section for HSE All users have a duty to familiarise themselves with the fire safety measures in the building This applies in particular to the location of fire exits fire extinguishing equipment and fire alarms In the event of a fire alarm Follow the general fire instructions Leave the building through the nearest fire exit and once outside do not stand by the entrance I have read and understood my instruction as a user of the buildings at the University of Oslo UiO Building Section Date User School of Pharmacy HSE manual Fire instructions FIRE INSTRUCTIONS FOR FIRE SAFETY SECTION LEADERS THE UNIVERSITY OF OSLO Fire instructions for floor and or section leaders Floor and or section leaders are responsible for Knowing the general fire instructions the instructions for abnormal and varying risk and local fire instructions if applicable Familiarising employees and students with fixed workplaces on their floor section with the general fire instructions and the user instructions In addition all escape routes and technical fire measures must be demonstrated When this information has been received this document must be signed by the recipient and then sent to the Head of Unit Providing new employees with the information above within
87. g of chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use Sashes of fume hoods may fall down and injure the person working in front of the hood Check the steel lines supporting sashes regularly Report immediately when there is damage with any lines and ask for repairing Occasional stop of ventilation may cause leak of toxic vapors from fume hood Be aware of the air flow with the fume hood all the time If ventilation stops lower down the sash and leave the room INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Emergency shower outside the door to the right e Eye wash flasks above the sink e Fire blanket Brannteppe above the sink e Fire extinguisher CO2 beside the sink e 3 fume hoods 1 occupied by rotavapor and vacuum pump 106 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0198 LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES e Handling of chemicals e Organic synthesis e Preparative chromatography Trond Vidar Hansen Trond Vidar Hansen e Use of instruments rotavapor vacuum pumps e Use of gas under pressure nitrogen and argon INSTRUMENTS AND EQUIPMENT Name type
88. g the supervision of electrical installations School of Pharmacy HSE manual Introduction Lov om vern mot brann eksplosjon og ulykker med farlig stoff og om brannvesenets redningsoppgaver brann og eksplosjonsvernloven Act relating to the prevention of fire explosion and accidents involving hazardous substances and the fire service The Fire and Explosives Act J Forurensningsloven The Pollution Control Act Genteknologiloven The Gene Technology Act Str levernloven Act on radiation protection and use of radiation School of Pharmacy HSE manual Introduction RESPONSIBILITIES AND DECISION TAKING STRUCTURE IN HSE ISSUES In general an attempt shall be made to resolve HSE related matters at the lowest possible level in the line in order to achieve local participation Responsibility follows the line from the Director School of Pharmacy to the Head of Department The immediate line manager at each level is responsible for ensuring that the instructions are known and complied with The immediate line manager is a scientific employee in a permanent position with responsibility for own research and or as student advisor for Master students research fellows guest researchers or who uses technical personnel in their research Instituttdirektor Administrasjonen Studie Vkonomi IT Avdeling for farmas ytisk biovitenskap Avdeling for farmas ytisk kjemi Avdeling for f
89. graphic chemicals 200117 7250 Asbestos insulation 170601 7091 Inorganic chemical remnants 160507 7030 Oil emulsions 120109 PACKING The chemicals are packed in boxes The box must first be equipped with a solid plastic bag Each box must only contain chemicals that have the same substance no The boxes containers must be tightly closed and packed vertically Boxes with plastic bags Waste containers for hazardous waste can be obtained from VWR low type with bag 50 L permitted weight 12 5 kg pack of 10 article no 129 0042 The Technical Department pays for these Several layers of small bottles can be packed in each box but a powder must also be added that not only neutralises but also absorbs water and knocks Perlite can be used which can be bought by the sack from Renor First place a little Perlite in the base of the box then spread a little between each layer of small bottles and finally a little on the top before closing NB Do not mix chemicals that can react with each other and develop poisonous gasses The worst case scenario is acids and cyanides that could generate hydrocyanic acid if the contents of the box break 22 School of Pharmacy HSE Manual Common routines DECLARATION The form Declaration of special waste can be bought from NORSAS Ui0 s organisation number is 971035854 Invoicing address Universitetet 1 Oslo Stedkode 32 20 12 Sentralt fakturamottak pb 1074 Blindern 0316 Oslo University of Oslo
90. he gas cylinder Turn 1 clockwise inwards turn 2 counter clockwise outwards Opening the gas cylinder Turn 1 counter clockwise outwards turn 2 clockwise inwards ACETYLENE If strange noises can be heard from a gas cylinder containing acetylene a chain reaction could be occurring inside the cylinder that can increase the pressure such that the cylinder bursts Acetylene is an extremely flammable gas Evacuate the building Contact the Fire services 27 School of Pharmacy HSE Manual Common routines INSTRUMENTS AND HAZARDS DEWAR VESSELS Danger of implosion due to internal vacuum Cold solutions must not be poured on the edge of the vessel HPLC HIGH PRESSURE LIQUID CHROMATOGRAPHY Overpressure can burst tubes resulting in solvent sample splash FRIDGES STANDARD Must not be used to store flammable chemicals due to the fact that sparks from the thermostat and light bulb present a fire and explosion hazard COMPRESSOR AND FLUID BED APPARATUS Take care to de pressurise tubes before dismantling LABORATORY DISHWASHERS Keep the area around the machine tidy Remove clean glass equipment when the machine has finished Examine and clean base and base filter Broken glass must be disposed of in the waste glass container NMR NMR instruments are surrounded by a magnetic field which can damage cards with magnetic strips e g mini bank cards key cards borrowing cards laptops strips data from the ha
91. ible person Training resposible person ROOM ACTIVITIES Trond Vidar Hansen Trond Vidar Hansen e Practical teaching of general and organic chemistry for lower degree students RULES e Synthesis products have to be labelled by date name producer product no journal no total amount chemical name and or structure and should be stored for later use INSTRUMENTS AND EQUIPMENT Training responsible person Name type Responsible person Comments 4 scales Tuliana Johansen Iuliana Johansen 3 heating ovens Iuliana Johansen Tuliana Johansen 1 UV lamp Tuliana Johansen Tuliana Johansen 2 melting point analyzers Tuliana Johansen Tuliana Johansen 20 magnetic stirrers w heating plate Tuliana Johansen Iuliana Johansen 20 heating mantles w magnetic stirring Iuliana Johansen Tuliana Johansen 40 cupboards of glassware Tuliana Johansen Tuliana Johansen 8 pH meters Tuliana Johansen Iuliana Johansen 6 desiccators w vacuum Iuliana Johansen Iuliana Johansen RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of A Read SOP or user s manual before use instruments and equipment Always wear eye protective glasses Consult the material safety data sheets for proper handling of Exposure to harmful chemicals chemicals Use personal safety equipment when handling chemicals
92. ing room storage A do o 111 BL24 285 A Chemicals Storage Room IRA dios 112 BE25 O18 Mill TN 113 BL25 037 Storage room coccion ouch siiis ineeie ee o eeen E EAEE E E E eE EEEE e e ri 113 BC25202 HPLC laboratory A ele hii sheets hein EAE does ete et 114 B25 203 LEMS laboratory aaa a a ile ule Glad ie ele enaa a aent hee ed 115 BL25 203 Capillary electrophoresis laboratory ooonnnnnccccnonoccnononcnnncnnonannononnnanoconnnnc conc ono nocnnnn ana crannnnncnnnes 116 BL25 207 Students lab courses laboratory oooooocccnnonocccnonaccnnnnnnnannonnnnnanocnnnnnnonon anno nn cnnnn ono cnnnn acc n rana ncnnnass 117 BE25 212 cl AS a ANE E EA EE TT R E a a 118 BE25 214 Students courses laboratoryo cinta A AR Ra he 119 A A e A iiei iti EAEE EE EREE EEE E E EEE AES SEES EE e TEE ESEE 120 AAA TA a a hotel hh see seeds ashen A Ee Eaa th 121 ALIAS ls AAA A AAA A AAAA 122 BL25227Conference TOM aria 123 BE25229 GC GCMS laboratory oortree TEA iii A A Atte 124 BLE25 314 Cooling TOM cola naaa a EE ETE E an ER Oise 125 BL25 316 Research laboratory cido oli NEE E V op T SEE EE n UP I SpE 126 BL25 318 Instrument laboratory cooooocccnnonccccnonanccnnnnonanncnnnonnnonnnnna nono nnnonnonnnnnno cnn nana c rana nora nano nernnnanicnnns 127 BL235 3307 KltcheVofliCe iii lo folle iio Sonn liso eh a 128 BL25 331 Laboratory cpu ueali AAAA thle loco tela AAAA AAA 129 B25 333 Laboratory aaa pd 130 BE25 3342 Instrumenta bierne ii AA A A aer VTEC S ESEE
93. ions in the applicable health safety and environment legislation The HSE Manual contains a chapter for each department in the School and for Administration The HSE Manual will be updated as required and as agreed with the secretary of the Local Working Environment Committee L AMU All employees and students are responsible for staying updated on the HSE regulations applicable at any time at the School School Of Pharmacy 19 12 2008 HSE GOALS AT THE SCHOOL OF PHARMACY The School of Pharmacy endorses the HSE goals of the University of Oslo UiO In order to achieve its goal of being a leading research university UiO must also be at the forefront within HSE with hazards under control and experiences under continuous development Hazards under control Experiences under continuous development This can be achieved by working towards Working environments for employees and learning environments for students that are completely safe and nurturing An organisation that is action taking including and learning Buildings that are accessible functional and safe Operations that are completely safe for the exterior environment LEGISLATION A few central laws that apply to HSE for additional laws refer to the HSE Manual for the Faculty of Mathematics and Natural Sciences Arbeidsmiljgloven The Working Environment Act Lov om tilsyn med elektriske anlegg og elektrisk utstyr Law of 24 May 1929 regardin
94. ith skin and if swallowed Very toxic danger of very serious irreversible effects through inhalation in contact with skin and if swallowed May cause sensitisation by inhalation and skin contact Harmful danger of serious damage to health by prolonged exposure through inhalation Harmful danger of serious damage to health by prolonged exposure in contact with skin Harmful danger of serious damage to health by prolonged exposure if swallowed Harmful danger of serious damage to health by prolonged exposure through inhalation and in contact with skin Harmful danger of serious damage to health by prolonged exposure through inhalation and if swallowed Harmful danger of serious damage to health by prolonged exposure in contact with skin and if swallowed Harmful danger of serious damage to health by prolonged exposure through inhalation in contact with skin and if Toxic danger of serious damage to health by prolonged exposure through inhalation Toxic danger of serious damage to health by prolonged exposure in contact with skin Toxic danger of serious damage to health by prolonged exposure if swallowed Toxic danger of serious damage to health by prolonged exposure through inhalation and in contact with skin Toxic danger of serious damage to health by prolonged exposure through inhalation and if swallowed Toxic danger of serious damage to health by prolonged exposure in contact with skin and if swallowed Toxic danger of serious damage to health
95. le Comments person Liquid nitrogen tank N2 Siri Johannesen 4 etg Cradle RISK ANALYSIS Risk Preventive measure Be careful when working with and filling of Risk of suffocation by emissions in confined liquid nitrogen BRACES Wear eye protection and appropriate gloves Need regular maintenance and check leakage INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Fire Blanket e Eyewash bottles 125 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 316 RESEARCH LABORATORY Room responsible person Hilde Barsett Training responsible person Supervisor ROOM ACTIVITIES e Research e Use of instruments REGLER e The users must keep things in order and tidy up e The users must remove paper and glass waste when it s needed e Fume cupboards that are not used shall have their hatches closed e The lab scales must be cleaned after use e Switch off the light in the end of the day INSTRUMENTS AND EQUIPMENT Name type Responsible person nai a Comments Rotavapor IKA VWR Suthajini Yogarajah Fraction collector Ellen Hanne Cohen Phenol sulfuric acid test equipment Laboratory scale RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of Read SOP or user s manual before use instruments and equipment Always wear eye protective glasses Exposure to harmful chemicals Consult
96. lies to pathogens e g virus that are associated with disease in humans Preventive or therapeutic measures are often available Work routines standard microbiological practice for BSL 2 Access to the laboratory is restricted when work with virus virus infect cells is ongoing The door into the laboratory and the LAF bench biosafety cabinet must be marked with the BSL 2 sign the name of the study and the duration of the study Personnel must have received training in working with viruses prior to granting authorisation a contract is drawn up that is signed contact the person responsible Work in the cell lab is performed in an LAF bench that is approved for such work Liquid waste such as medium solutions containing virus etc is disposed of into flasks containing Virkon solution that are situated in the LAF bench For suction the Virkon solution is added to the collection chamber liquid waste is autoclaved and can then be disposed of down the sink Pipettes pipette tips etc are placed in a dedicated waste bucket see below Pipettes must be rinsed in Virkon solution before they are put in the waste bucket Mark incubators with the virus sign during incubation Work with the cells in accordance with the BSL 2 standard until they have been lysed with sodium hydroxide or other SDS solution chloroform methanol Frozen cells in water should be treated as BSL 2 Disinfection decontamination Virus waste is disinfected wi
97. m responsible person Training responsible person ROOM ACTIVITIES e Research e Use of instruments RULES e General laboratory rules INSTRUMENTS AND EQUIPMENT Ewa Jaroszewicz Ewa Jaroszewicz Name type Responsible person Training responsible person Notes 2 PCR blocks Ewa Jaroszewicz Ewa Jaroszewicz 3 Table centrifuges Heraeus Ewa Jaroszewicz Ewa Jaroszewicz UV transmission apparatus Fluo Link from Vilber Lourmat Ewa Jaroszewicz Ewa Jaroszewicz Fume hood Normodul Ewa Jaroszewicz Ewa Jaroszewicz Distillation apparatus Ewa Jaroszewicz Ewa Jaroszewicz Water bath Ewa Jaroszewicz Ewa Jaroszewicz Microwave Ewa Jaroszewicz Ewa Jaroszewicz Heating blocks Ewa Jaroszewicz Ewa Jaroszewicz RISK ANALYSIS Risk Precaution Exposure to harmful chemicals Read Safety Data Sheets in advance Use safety equipment Work in fume hood Clean up spillage of chemicals immediately Work with an open gas flame Use with coution Ensure that the gas burners work properly They must not burn unnecessary and they shall be closed when leaving the laboratory INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY Emergency shower Fire equipment Fire blanket Eye wash bottles 78 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 05 11 2010 BL21 168 INSTRUMENT ROOM
98. mful Dangerous for the R50 R50 53 R51 53 R54 R55 N Dangerous for the Dangerous for the environment R56 R57 R58 R59 environment environment pictogram R52 R52 53 R53 None None None 141 School of Pharmacy HSE Manual Appendices HAZARD CLASSES CRITERIA FOR HSE ASSESSMENT OF CHEMICALS oo MS ve Not acceptable HSE HAZARD CLASS Factor datasheet Lowest hazard class Highest hazard class assessed section NO L abe X Harmful T Toxic E ee 15 i Xi Irritant C Corrosive T Very toxic A mandatory mandatory R43 R42 F Extremely F Highly flammable Ee zata flammable O Oxidising E Explosive YL group 15 x N Dangerous External ee ol for the 15 52 R5 for the environment ama RaD environment R50 YL Occupational Health and Hygiene ventilation requirements GAS CYLINDERS COLOUR CODES UiO s gas cylinder supplier AGA has introduced shoulder marking on the gas cylinders in accordance with the new European standard EN 1089 3 The shoulder marking only provides information about the gasses properties in air but most pure gasses have been allocated a dedicated colour More detailed information on the gas cylinder content is to be found on the product label SHOULDER COLOUR EN 1089 3 GENERAL Toxic and or corrosive gasses Yellow Flammable gasses Red Oxidising gasses Light blue Inert gasses Bright green SHOULDER COLOUR EN 1089 3 PURE G
99. minescence or fluorescence quantifying western northern and southern blots RULES New users have to contact Tor Gj en for training and a review of the procedures You have to keep your own cassettes They are expensive and are easily broken Makes sure that western southern blot are wrapped in plastic before they are put in the cassette or are scanned Refer to the instrument manual for information on functions sensitivity and use of the scanner before you plan to scan your plots You will find the manual at the instrument Do not scan with higher resolution than 200 um Your data files will get bigger but they will not give you better quality Log on and save your scans on your own directory Install the image editing software on your own computer and edit your images in your office INSTRUMENTS Fume cupboard with a drain Molecular Dynamics Storm 860 scanner Computer Lamp for resetting cassettes Instrument responsible person Tor Gjgen 60 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 1U51 COURSE ROOM Last update 21 06 2010 Room responsible person Training responsible person ROOM ACTIVITIES e Courses RULES e General laboratory rules INSTRUMENTS AND EQUIPMENT Ewa Jaroszewicz Course supervisor Engineer Name type Responsible person Training responsible person Notes Various equipment Ewa Jaroszewicz Cours supervisor Engin
100. mmable explosive vapour air mixture May form explosive peroxides Harmful by inhalation Harmful in contact with skin Harmful if swallowed Toxic by inhalation Toxic in contact with skin Toxic if swallowed Very toxic by inhalation Very toxic in contact with skin Very toxic if swallowed Contact with water liberates toxic gas Can become highly flammable in use Contact with acids liberates toxic gas Contact with acids liberates very toxic gas Danger of cumulative effects Causes burns Causes severe burns Irritating to eyes Irritating to respiratory system Irritating to skin Danger of very serious irreversible effects Limited evidence of a carcinogenic effect Risk of serious damage to eyes May cause sensitisation by inhalation May cause sensitisation by skin contact Risk of explosion if heated under confinement May cause cancer May cause heritable genetic damage Danger of serious damage to health by prolonged exposure May cause cancer by inhalation Very toxic to aquatic organisms Toxic to aquatic organisms Harmful to aquatic organisms May cause long term adverse effects in the aquatic environment Toxic to flora Toxic to fauna Toxic to soil organisms Toxic to bees May cause long term adverse effects in the environment Dangerous for the ozone layer May impair fertility May cause harm to the unborn child Possible risk of impaired fertility Possible risk of harm to the unborn child May cause harm to brea
101. mpulsory for all employees Net based laboratory safety courses are compulsory for all Master thesis students The person responsible for the substances and chemicals register can provide training in the use of the net based substances and chemicals register HSE datasheet All types of training and times are listed on the School s HSE web page HSE WEB PAGES UiO HSE web page http www uio no for_ansatte ansatt arbeidsmiljo Faculty of Mathematics and Natural Sciences web page School of Pharmacy HSE web page http www farmasi uio no ansatte HMS html FORMS Forms can be found via the School of Pharmacy s HSE web page or in the appropriate HSE Manual School of Pharmacy HSE manual First aid rules FIRST AID RULES GENERAL RULES IN THE EVENT OF INJURY Immediately inform a member of staff In the event of a chemical accident you must report the chemical s involved SPLASHES IN THE EYES Rinse continuously with water preferably from an eye douching bottle Continue rinsing while en route to the doctor and while waiting at Ulleval University Hospital until seen by a doctor in the Ophthalmology department Remember to take additional eye douching bottles with you SPILLAGE ON SKIN AND CLOTHES Rinse with water for at least 15 mins Wash with soap and water Remove soiled clothes shoes and jewellery Skin contact with wet clothes can often cause worse injury than spills directly on the skin Therefore it is important
102. n Keep in a cool place Keep away from living quarters Keep contents under appropriate liquid to be specified by the manufacturer importer or distributor Keep contents under inert gas to be specified by the manufacturer importer or distributor Keep container tightly closed Keep container dry Keep container in a well ventilated place Do not keep the container sealed Keep away from food drink and animal feeding stuffs Keep away from incompatible materials to be indicated by the manufacturer importer or distributor Keep away from heat Keep away from sources of ignition No smoking Keep away from combustible material Handle and open container with care When using do not eat or drink When using do not smoke Do not breathe dust Do not breathe gas fumes vapour spray appropriate wording to be specified by the manufacturer importer or distributor Avoid contact with skin Avoid contact with eyes In case of contact with eyes rinse immediately with plenty of water and seek medical advice Take off immediately all contaminated clothing After contact with skin wash immediately with plenty of to be specified by the manufacturer importer or distributor Do not empty into drains Never add water to this product Take precautionary measures against static discharges This material and its container must be disposed of in a safe way Wear suitable protective clothing Wear suitable protective gloves In case
103. ndbook for Students must be notified to the person responsible for reporting remedy All orders from the Safety Delegate must be complied with UNAUTHORISED PERSONS Notify the Security Centre if unknown persons are discovered in the laboratory or on other premises to which they do not have access From and including 01 10 2002 employees are permitted to restrain request proof of identity NB Minors are not permitted in the laboratories 25 School of Pharmacy HSE Manual Common routines SAFETY DATASHEETS AND THE SUBSTANCES AND CHEMICALS REGISTER Safety datasheets must be read prior to opening any chemicals container Be aware that the contents of a new chemical container of a specific product can be more reactive than the contents of an old one The School of Pharmacy will via a person responsible for the Substances and Chemicals Register in each department maintain the School s collection of electronic safety datasheets and localisation of the School s chemicals ECOonline Substances and Chemicals Register The user must inform the person responsible for the Substances and Chemicals Register of any changes in the chemicals stock The users groups must maintain a separate or joint binder with material safety data sheets in hard copy on site If the updated safety data sheets are missing in their own binder a new version should be printed from the School s electronic Substances and Chemicals Register ECOonline BIOLOGICAL MA
104. nearby e Fire extinguisher e Fire Blanket e First aid kit e Eyewash bottles e Chemical Cabinets w exhaust 133 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 339 B CHROMATOGRAPHY LAB Room responsible person Training responsible person ROOM ACTIVITIES Suthajini Yogarajah Supervisor e Student Course e Research e Use of instruments RULES TO FOLLOW User must keep order and clean up after themselves Safety cabinets that are not in use shall be closed Chemicals should not be stored outside the chemical cupboard fridge Chemical spills be removed immediately e Turn off lights when leaving room for the day INSTRUMENTS AND EQUIPMENT Training Name type Responsible person responsible person Comments TLC tank Suthajini Yogarajah TLC spray Camag Suthajini Yogarajah UV equipment Suthajini Yogarajah Ultrasonic VWR Suthajini Yogarajah Heating cabinet Heraeus Suthajini Yogarajah eee Karl Malterud RISK ANALYSIS Risk Preventive measure Exposure to hazardous chemicals Use safety data sheets for proper handling and storage of each chemical INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY 134 Emergency shower nearby Fire extinguisher Fire Blanket Eyewash bottles Chemical Cabinets w exhaust School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 340 PREPARATION ROOM COURSES
105. ngs made from resistant materials The room must be dry and well ventilated Emergency showers eye douches adsorption agents and fire extinguishing devices of the correct type must be located in the immediate vicinity of the storage room The storage room must be inspected regularly The storage room must be marked in accordance with the regulations on safety signs and signals in the workplace 3 3 GENERAL SAFETY REGULATIONS In general chemicals must be stored on the first in first out principle to avoid exceeding their expiry date If the chemicals do not have a date stamp on the packaging the date of reception must ALWAYS be noted on the chemical before placing in stores 3 4 INCOMPATIBLE CHEMICALS Incompatible chemicals must be stored apart in a dedicated storage facility A dedicated storage facility is an arrangement that prevents contact between the chemicals in the event of leakage Small amounts of incompatible chemicals lt 100 ml may be stored in the same facility if a risk assessment has been conducted and suitable secondary containment is used 3 5 FLAMMABLE SUBSTANCES LIQUIDS AND GASSES Flammable substances liquids and gasses must be stored in fireproof cabinets or rooms The cabinet or room must be marked with a fire hazard sign The container for storing flammable goods must be in a secure location Accessibility must be facilitated in connection with fire fighting status checks and maintenance Flammable gasses
106. nt and for decontamination INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanket e Fire extinguisher e First aid kit 116 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 207 STUDENTS LAB COURSES LABORATORY Room responsible person Finn Tgnnesen Training responsible person ROOM ACTIVITIES e Students lab courses INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Comments pH meter Metrohm 827 Finn Scale Mettler Toledo Finn Laboratory washing machine Finn Ultrasonic bath Branson 5510 Finn RISK ANALYSIS Risk Preventive measure Health hazard by doing mistake while working in a fume hood Refer to the Common Routine chapter Correct Use of Fume Cupboards Risk of water leakages Turn out the water supply to the water distillating apparatus at the end of the day Do not use the distillating apparatus or the lab washer after normal working hours Exposure to harmful chemicals Consult the material safety data sheets for proper handling of chemicals INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanket e Fire extinguisher e Eye washer 117 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 212 BALANCE ROOM Room responsible person Finn Tgnnesen Training responsible person ROOM ACTIVITIES
107. nts injuries 99 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 K82 CHEMICALS STORAGE ROOM Room responsible person Atle Haugen Training responsible person Atle Haugen ROOM ACTIVITIES e Deposit and withdrawal of chemicals e Local reception of chemical waste RULES e Deposit withdrawal and destruction of chemicals has to be registered in accordance with the lists on the door INSTRUMENTS AND EQUIPMENT Name type Responsible person Training POE Comments person RISK ANALYSIS Risk Preventive measure Use personal safety equipment when handling Exposure to harmful chemicals b chemicals Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use Synthesis products have to be labelled by date name producer product no journal no total amount chemical name and or structure Smell and unwanted incidents accidents in the Containers of chemicals should be transported in secure corridors vessels or trolleys HSE INSTALLATIONS e Emergency shower right outside the door e Fire extinguishing equipment and fire blanket e Eye wash flasks e Absorbent for spilled chemicals and liquids 100 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL24 0187 STUDENTS COURSES LABORATORY Room responsible person Training resposible person
108. of insufficient ventilation wear suitable respiratory equipment Wear eye face protection To clean the floor and all objects contaminated by this material use to be specified by the manufacturer importer or In case of fire and or explosion do not breathe fumes During fumigation spraying wear suitable respiratory equipment appropriate wording to be specified by the manufacturer importer or distributor In case of fire use the manufacture importer or distributor must indicate in the space the precise type of fire fighting equipment If water increases the risk add Never use water In case of accident or if you feel unwell seek medical advice immediately show the label where possible If swallowed seek medical advice immediately and show this container or label Keep at temperature not exceeding C to be specified by the manufacturer importer or distributor Keep wet with appropriate material to be specified by the manufacturer importer or distributor Keep only in the original container Do not mix with to be specified by the manufacturer importer or distributor Use only in well ventilated areas Not recommended for interior use on large surface areas Avoid exposure obtain special instructions before use Dispose of this material and its container at hazardous or special waste collection point Use appropriate containment to avoid environmental contamination Ensure safe packaging to prevent environmental c
109. ontamination Refer to manufacturer importer or distributor for information on recovery recycling This material and its container must be disposed of as hazardous waste Avoid release to the environment Refer to special instructions HSE safety data sheet for further information If swallowed do not induce vomiting seek medical advice immediately and show this container or label In case of accident by inhalation remove casualty to fresh air and keep at rest If swallowed rinse mouth with water only if the person is conscious Use compressed air or fresh air line breathing apparatus in confined spaces 145 School of Pharmacy HSE Manual Appendices SAFETY PHRASES IN COMBINATION When several phrases are combined in one sentence the phrases must be as specified below The combination 1s considered to be one sentence s1 2 S3 7 S3 9 14 S3 9 14 49 S3 9 49 S3 14 distributor S7 8 S7 9 S7 47 820 21 824 25 827 28 829 35 S29 56 S36 37 36 37 39 36 39 837 39 S47 49 146 Keep locked up and out of the reach of children Keep container tightly closed in a cool place Keep in a cool well ventilated place away from to be specified by the manufacturer importer or distributor Keep only in the original container in a cool well ventilated place away from incompatible materials to be indicated by the manufacturer importer or distributor Keep only in the original container in a cool well ventilated
110. oratory These containers must be labelled Halogenated organic solvent remnants Examples of such substances are carbon tetrachloride chloroform methylene chloride trichlorethylene chlorbenzene etc as well as nitrobenzene NON HALOGENATED SOLVENTS Remnants of non halogenated organic solvents must be emptied into the remnants containers located in each laboratory These containers must be labelled Non halogenated organic solvent remnants VOLATILE ORGANIC SOLVENT REMNANTS BENZENE ETHER ETC Small quantities can be evaporated off in the fume cupboard without application of heat or outdoors in an isolated area When the organic solvent waste remnant containers are full they must be emptied into the collection tanks in the Workshop yard at the Department of Chemistry see picture Cans bottles with non halogenated solvent remnants are emptied into the left tank Cans bottles with halogenated solvent remnants are emptied into the right tank Remember to note what you have emptied in the books in the cupboard by the tanks Use protective eye goggles also to be found in the cupboard The key to the tanks can be obtained from Central Stores or the engineer in the Medicinal Chemistry group If the collection tank for the non halogenated waste is full the waste can be disposed of together with the halogenated waste Halogenated waste must NEVER be disposed of into the collection tank for non halogenated waste 20 School o
111. place Keep in a cool place away from incompatible materials to be indicated by the manufacturer importer or Keep container tightly closed and dry Keep container tightly closed and in a well ventilated place Keep container tightly closed and at temperature not exceeding C to be specified by the manufacturer importer or distributor When using do not eat or drink or smoke Avoid contact with skin and eyes After contact with skin take off immediately all contaminated clothing and wash immediately with plenty of to be specified by the manufacturer importer or distributor Do not empty into drains dispose of this material and its container in a safe way Do not empty into drains dispose of this material and its container at hazardous or special waste collection point Wear suitable protective clothing and gloves Wear suitable protective clothing gloves and eye face protection Wear suitable protective clothing and eye face protection Wear suitable gloves and eye face protection Keep only in the original container at temperature not exceeding C to be specified by the manufacturer importer or distributor School of Pharmacy HSE Manual Appendices ROUTINE TEMPLATE FOR ROOM BL2X XXX XXXXX The routine was last updated on 11 05 2010 Main Room responsible xxxxx Training responsible XXXXX ACTIVITIES XXXXX RULES XXXXX INSTRUMENTS EQUIPMENT Responsible for Name type Instrumen
112. r 76 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 166 RESEARCH LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES e Research work Mona Gaarder Mona Gaarder INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments Centrifuge Biofugo pico Mona Gaarder Mona Gaarder Centrifuge Biofugo fresco Mona Gaarder Mona Gaarder Gel doc Biorad Mona Gaarder Mona Gaarder 2 water baths Mona Gaarder PTC machine Anne Lise Rishovd Anne Lise Rishovd ABI 6100 Nucleic Acid Anne Lise Rishovd Anne Lise Rishovd Prepstation ABI 2720 Thermed Cycler Anne Lise Risovd Anne Lise Rishovd RISK ANALYSIS Risk Preventive measure Gas jet Be careful in use Read the safety data sheet before handling chemicals Always wear a lab coat and gloves If Chemicals necessary also use protective goggles and a face mask Biological Use labcoat and gloves Avoid any spillage material Pathogenic Use labcoat and gloves Avoid any spillage bacteria INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanket and fire extinguisher e Eye washer e Fume hood e Ventilated chemical locker 77 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 167 RESEARCH LABORATORY Last update 21 06 2010 Roo
113. ration INSTRUMENTS AND EQUIPMENT Hilde Nilsen phone 56547 Hilde Nilsen Name type Responsible person ae fesponsible Comments Sartorius basic x2 Hilde Nilsen Hilde Nilsen Sartorius 1205 P Hilde Nilsen Hilde Nilsen Ohaus Model TS 120S Hilde Nilsen Hilde Nilsen Mettler Toledo Hilde Nilsen Hilde Nilsen pH meter PHM 210 Hilde Nilsen Hilde Nilsen Miele oppvaskmaski Hilde Nilsen Hilde Nilsen RISK ANALYSIS Risk Preventive measure Chemicals Use mask and gloves HSE INSTALLATIONS e EYE WASH BOTTLE e FIRE EXTINGUISHER 86 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 08 11 2010 BL25 411 HPLC ROOM Room responsible person Training responsible person ROOM ACTIVITIES e Sentrifugation e Microsome isolation e Cell experiments Siri Johannesen office 475B phone 56554 Siri Johannesen e Metabolism experiments e Sample preparation e HPLC INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Comments HPLC Perkin Elmer Siri Johannesen Siri Johannesen HPLC LKB Siri Johannesen Siri Johannesen Supelco Phase extraction Siri Johannesen Siri Johannesen Optima ultra sentrifuge Siri Johannesen Siri Johannesen Agilent HPLC Anders Aasberg Anders Aasberg RISK ANALYSIS Risk Pr
114. rd disc clocks electronic equipment People with pacemakers and those who have implants implanted parts of magnetic metal in the body are not allowed to enter the NMR lab This is for their own safety since the pacemaker may stop functioning ROTAVAPOR Read the entire user instructions prior to using the rotavapor Follow the safety instructions in the SOP The rotavapor must only be used by employees and master students who either through training or own professional experience have a sound understanding of the dangers that can arise at use of the equipment Those who have not received training must not use the rotavapor by themselves Round bottomed flasks with cracks hairline cracks must not be used as they can implode explode inwards due to the vacuum Rotavapors should preferably be connected to membrane pumps with cold traps and exhaust to the fume cupboard and should not be connected to a water jet pump where much of the solvents that evaporate will disappear down the drain Personal protective equipment such as eye protection and lab coats must always be used when working with a rotavapor Hard copies of the user instructions and the SOP standard operating procedure for the type of rotavapor must always be available next to the equipment Refer to the School of Pharmacy common area NAHMS for the following user instructions for use of the rotavapor Biichi Rotavapor Operation Manual pdf This contains a good description of correct
115. rely tightened gas Gas jet Be careful when you use it e Read the safety data sheet before handling chemicals Always wear a lab coat and gloves If Chemicals necessary also use protective goggles and a face mask Biological Use a lab coat and gloves material INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguishing equipment e Eye washer 69 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 152 COLD ROOM Room responsible person Training responsible person ROOM ACTIVITIES e Reasearch activites e Store room e N2 tank Anne Lise Rishovd Research groups INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments BioRad Power Pac 300 Anne Lise Rishovd Research groups Horisontal shaker GFL 3005 Anne Lise Rishovd Research groups Roller Mixer SRT2 Stuart Scientific Anne Lise Rishovd Research groups Centrifuge Wifug Anne Lise Rishovd Research groups Roller Labinco Anne Lise rishovd Research groups LS4800 Taylor Mona Gaarder Mona Gaarder Wharton Nitrogen tank RISK ANALYSIS Risk Preventive measure Fluid nitrogen Protective plexiglas mask insulated gloves coat Methanol contaning solutions Nitril gloves protective glasses In general GLP Good Laboratory Pratice HSE INSTALLATIONS e Safety equipment for h
116. responsibility remains with the floor department leader I have read and understand the instructions for floor department leader Building floor unit Date Floor and or department leader 13 School of Pharmacy HSE Manual Common routines COMMON ROUTINES ACTION PLAN IN THE EVENT OF SERIOUS ACCIDENTS INCLUDING MANDATORY NOTIFICATION TO THE NORWEGIAN LABOUR INSPECTION AUTHORITY PURPOSE To ensure that necessary first aid is given and to ensure that the correct units are notified in the event of serous personal injury including notification to the Norwegian Labour Inspection Authority To prevent new accidents and injury RESPONSIBILITY The immediate line manager at the site of the accident incident is responsible for following up the accident incident both with regard to the injured and with regard to notification on the appropriate form DEFINITION e Serious accident An undesired incident that results in serious injury to people the environment or materials e Accident An undesired incident that results in minor injury to people the environment or materials e Near accident An undesired incident that in slightly different circumstances would have resulted in injury to people the environment or materials PROCEDURE ACTION PLAN IN THE EVENT OF SERIOUS ACCIDENTS INJURY 1 Give first aid UiO Health Services Helsetjenesten tel 22 85 31 74 2 Notify the Security Centre at UiO tel 22 85 66 66 who will forward
117. responsible Comments Dishwasher Ing farmakognosi PC Ellen Hanne Cohen Skanner Ellen Hanne Cohen Printer Ellen Hanne Cohen RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY 128 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 331 LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES e Research e Use of instruments RULES Berit Smestad Paulsen Supervisor e The users must keep things in order and tidy e Switch off the lights in the end of the day INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Comments Rotavapor Suthajini Yogarajah Plateleser Suthajini Yogarajah RISK ANALYSIS Risk Preventive measure Exposure to harmful chemicals Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Fire blanket e Eye washer e Chemical waste containers 129 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 333 LABORATORY Room responsible person Berit Smestad Paulsen Training responsible person Supervisor ROOM ACTIVITIES e
118. rmacy Administration 35 School of Pharmacy HSE Manual Administration TABLE OF CONTENTS ERSA a T e D AEE ER PEPE A E E E E E 36 Receptionen nr np e r a S V A is 36 Staff ISSUES nann aco a apa ios bd 36 Co rses examinati esiritta anaiei e e eeina a a a a aa a a r Eeen e ar ineeie ENET 36 Curricular aaa AA anna eee daras 36 IN GENERAL This is an overview of the routines in the Administration that are relevant as for the HSE work You will find an overall presentation of the routines in the Administration at N Administrasjonens_Rutinehandbok_kopi RECEPTION 1 2 Admission card 1 3 Keys 1 9 Dealing with pure alcohol 1 13 Preparing the teaching rooms 1 15 Students events 1 16 Fire instructions at events in the room BL25 054 055 1 20 Contact with the Technical Department STAFF ISSUES 3 3 Registering new employees 3 4 Absence registration 3 6 Self certified sick leave doctor s certificate 3 7 The handling of issues involving staff and teaching problems 3 8 Quitting approvement 3 10 Reporting deaths COURSES EXAMINATION 6 7 Fire alarm during examination 6 11 Complaint about the censorship CURRICULUM 7 4 Evaluation of subjects 36 School of Pharmacy HSE Manual Department of Pharmacy ROUTINES AT THE DEPARTMENT OF PHARMACY HSE Health Safety and the Environment in the workplace Version 2010 Universitety of Oslo School of Pharmacy Department of Pharmacy 37 School o
119. rming bacteria Use safety equipment INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e No 54 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 2U53 ISOTOPE ROOM CLASS B Last update 01 11 2010 Room responsible person Ewa Jaroszewicz Training responsible person Ewa Jaroszewicz local radiation protection coordinator for ZEB ROOM ACTIVITIES e Work with isotop Loj 2p 355 RULES e General rules for isotoplaboratory class B INSTRUMENTS AND EQUIPMENT 3 Training responsible Name type Responsible person 8 resp Notes person Beta Gamma counter o E Ewa jaroszewicz Ewa Jaroszewicz scintallation counter Geiger Muller detector Ewa Jaroszewicz Ewa Jaroszewicz Vertical electroforese Ewa Jaroszewicz Research group RISK ANALYSIS Risk Precaution Exposure to radioactive materials All users must have completed an approved course Read Safety Data Sheet and radiation protection regulations in advance All users have to wear personaldosimeter Use safety equipment when handling radioactive materials Use 1 cm perspex Plexiglass as shilding Work in fume hood Regular monitoring for contamination is required INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Eye wash bottles e Plexiglass shield e Plexiglass boxes for radioactive waste 55 School of Pharmacy HSE Manual Depar
120. secondary containment Stock solutions in small amounts can be stored in the laboratories 3 13 CORROSIVE SUBSTANCES Corrosive substances must always be stored as low as possible and in all circumstances below head height This is to reduce the risk of personal injury in the event the container should tip over 3 14 GASSES Gas cylinders must always be stored upright and must be secured with a chain or similar to avoid tipping of the cylinder The chain must be affixed above the cylinder s centre of gravity Rooms in which pressurised gas is stored must be marked with the correct hazard symbol This is to avoid the risk of personal injury in particular in the event of a fire to fire fighting personnel 4 TRAINING The Head of Department must ensure that all employees are familiar with the requirements and regulations for storing chemicals and that necessary instruction is given 19 School of Pharmacy HSE Manual Common routines 5 DISPOSAL OF CHEMICAL WASTE 5 1 GENERAL One of the engineers in the subject group Medicinal Chemistry Department of Pharmaceutical Chemistry is responsible for the handling of chemicals and implementation of the routines described in Chapter 5 Chemical waste that can be destroyed simply in an environmentally and responsible way in the unit must not be handed in but is to be destroyed locally Destruction in the laboratory Use the procedures described in Kjemiske stoffer Chemical substances
121. ssess whether preventive measures are to be instigated 5 Ifthe scope of the incident is greater than that outlined above the routines for mandatory notification to the Norwegian Labour Authorities of accidents in the workplace with serious personal injury must be followed 6 For all personal injury requiring medical assistance sick leave and for injuries that could result in future incapacity even if medical assistance is not called the immediate line manager must ensure that RTV Form 13 07 05 is sent to the Norwegian Labour and Welfare Administration The form can be obtained from the Social Security Office or the HSE Section PO 1071 Blindern 7 The Norwegian Labour and Welfare Administration s RTV Form 13 07 05 must also be sent to the State Pension Fund if the employee is a member there in accordance with the guidelines in section 5 8 Consent must be obtained from the injured person to enable use of the personal details from the accident in internal accident prevention efforts The Working Environment Act 20 If consent is not given the form must be stored confidentially in the injured person s company medical records 16 School of Pharmacy HSE Manual Common routines THE LABORATORY SAFETY AND THE WORKING ENVIRONMENT ROUTINES FOR HANDLING AND STORING CHEMICALS AT THE SCHOOL OF PHARMACY UNIVERSITY OF OSLO Version 17 04 2009 P Rongved PURPOSE The routines describe the general guidelines for handling
122. st fed babies Harmful may cause lung damage if swallowed Repeated exposure may cause skin dryness or cracking Vapours may cause drowsiness and dizziness Possible risk of irreversible effects 143 School of Pharmacy HSE Manual Appendices RISK PHRASES IN COMBINATION When several phrases are combined in one sentence the phrases must be as specified below The combination 1s considered to be one sentence R14 15 R15 29 R20 21 R20 22 R20 21 22 R21 22 R23 24 R23 25 R23 24 25 R24 25 R26 27 R26 28 R26 27 28 R27 28 R36 37 R36 38 R36 37 38 R37 38 R39 23 R39 24 R39 25 R39 23 24 R39 23 25 R39 24 25 R39 23 24 25 R39 26 R39 27 R39 28 R39 26 27 R39 26 28 R39 27 28 R39 26 27 28 R42 43 R48 20 R48 21 R48 22 R48 20 21 R48 20 22 R48 21 22 R48 20 21 22 swallowed R48 23 R48 24 R48 25 R48 23 24 R48 23 25 R48 24 25 R48 23 24 25 swallowed R50 53 R51 53 R52 53 R68 20 R68 21 R68 22 R68 20 21 R68 20 22 R68 21 22 R68 20 21 22 144 Reacts violently with water liberating extremely flammable gasses Contact with water liberates toxic extremely flammable gases Harmful by inhalation and in contact with skin Harmful by inhalation and if swallowed Harmful by inhalation in contact with skin and if swallowed Harmful in contact with skin and if swallowed Toxic by inhalation and in contact with skin Toxic by inhalation and if swallowed Toxic by inhalation in contact with skin and if swallowed Toxic in contact
123. t e Fire extinguisher e Eye washer 120 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 220 CONFERENCE ROOM Room responsible person Finn Tgnnesen Training responsible person ROOM ACTIVITIES e Conference room INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible Comments person RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY 121 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 222 LABORATORY Room responsible person Training responsible person ROOM ACTIVITIES e Lab INSTRUMENTS AND EQUIPMENT Name type Training a responsible person Comments Atomic absorption spectrophotometer Ragnar RISK ANALYSIS Risk Preventive measure INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire blanket e Fire extinguisher 122 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 227 CONFERENCE ROOM Room responsible person Finn Tgnnesen Training responsible person ROOM ACTIVITIES e Conference room INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible Comments person RISK ANALYSIS Risk Preventive measurement INSTALLATIONS REGARDING HEALTH ENVIRON
124. t use refer to ECOonline INSTRUMENTS AND EQUIPMENT Training r nsibl Name type Responsible person aiming responsible Comments person NMR instrument 2 65 MHz Juliana Johansen Atle P l Roneved Bruker MiniSpec Haugen 8 C system VersaFlash luliana Johansen luliana Johansen Sentrifuge Heraeus Sepatech luliana Johansen Atle luliana Johansen Atle Haugen Haugen Water bath w shaking luliana Johansen Atle luliana Johansen Atle Haugen Haugen Water purifying instrument To prevent any water leakages ELGA Prima and Maxima Tuliana Johansen Tuliana Johansen please close the dispenser properly SA n Labsonic U ultra sonic luliana Johansen luliana Johansen Coulter Electronics rolling turning robot Atle Haugen Atle Haugen Metrohm 748 Stat Titrino Atle Haugen Atle Haugen titrator RISK ANALYSIS Risk Preventive measure Exposure to harmful s a chemicals Use personal safety equipment when handling chemicals Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker Work benches must be cleaned after use Synthesis products have to be labelled by date name producer product no journal no total amount chemical name and or structure HSE INSTALLATIONS e Emergency shower to the left outside the door e Fire extinguishing equipment and fire blanket e Eye wash flasks e Labelled emergency exit e List of phone numbers at accide
125. terials are appropriate and that necessary safety equipment is available and functioning e The quantity of chemicals in the fume cupboard must be limited to that necessary for the work in progress 24 School of Pharmacy HSE Manual Common routines PERSONAL PROTECTIVE EQUIPMENT Personal protective equipment must be available in the laboratory and must be used if conditions dictate this 1 e eye protection gloves and laboratory coat Face protection is shared between the laboratories as required Other personal protective equipment e g breathing apparatus can be obtained as required Use a fume cupboard if the work requires this Contact lenses can be worn 1f they are used in combination with eye protection FIRST AID EQUIPMENT First aid equipment must be easily accessible in each laboratory 1 An emergency shower must be installed just outside every laboratory Eye douches Research laboratories Minimum one per person Course laboratories Minimum one per 3 persons 3 First aid cupboard box must as a minimum contain Plasters scissors all in one packs bandages 1 bottle chlorhexidine 4 Fire blankets Minimum 1 fire blanket in each laboratory LABORATORY TESTS Laboratory tests must undergo risk assessment and be recorded Risk assessment A systematic assessment to find out which undesired events could occur what could be done to prevent these and what is necessary to minimise the impact if something should neverth
126. th Virkon solution Follow the instructions for use www puls norge no Suction and tubes must be rinsed with Virkon solution and then with 70 ethanol The LAF bench must be first washed with 2 SDS solution and then with 70 ethanol Leave the LAF bench under UV light overnight BSL 2 waste Waste pipettes cell flasks medium must be put in red bags marked hazardous waste The bags are placed in stainless steel metal containers that must be used only for this type of waste All waste must be autoclaved After autoclaving the waste can be disposed of as hazardous waste in yellow boxes When the boxes are full they must be closed properly labelled and taken to the Pharmacy building Room 009 33 School of Pharmacy HSE Manual Common routines RADIOACTIVE WASTE All waste that has been in contact with radioactivity must be put in special waste boxes There must be one box for C and one for H waste Non radioactive hazardous waste must not be disposed of together with radioactive waste Waste from work with P must be disposed of in plexiglass containers For collection of the waste contact the local Arild Rustan for FI Ewa Jaroszewicz for ZEB or central Radiation Safety Coordinator Elin Agathe Hult at UiO 34 School of Pharmacy HSE Manual Administration ROUTINES AT THE ADMINISTRATION HSE Health Safety and the Environment in the workplace Version 2010 Universitety of Oslo School of Pha
127. the laboratories must be stored on designated shelves or in designated cupboards Impermeable packaging must be used Liquids oils must be stored in ventilated cupboards Chemicals must not be stored on workbenches or in fume cupboards 3 9 CHEMICALS STORED IN A FRIDGE FREEZER Fridges are primarily to be used for chemicals that are temperature sensitive If the fridge does not have ventilation the chemicals must be stored in suitable containers jars Packaging must as far as possible be impermeable Round bottomed flasks and Erlenmeyer flasks must if necessary only be used for a limited period days and must be closed Chemicals with a limited shelf life must be destroyed at expiration of the shelf life not stored At least once very six months fridges and cold rooms must be emptied of chemicals that are not going to be used in the near future 3 10 IN HOUSE MANUFACTURED CHEMICALS A suitable closed container must be used to store in house manufactured chemicals The container must be labelled with the name of the substance its structure register no production no date and the identity of the person responsible 3 11 ETHANOL Ethanol must be stored in a locked cupboard Stock solutions in small amounts can be stored in the laboratories 3 12 ACIDS AND BASES Acids and bases must be stored in dedicated cupboards that are marked with the correct hazard symbol Acids and bases must always be stored separately and be placed in suitable
128. the material safety data sheets for proper handling of chemicals Use personal safety equipment when handling chemicals Never conduct any experiments outside the fume hoods Clean up spillage of chemicals immediately Always store chemicals in the chemical storage locker HMS INSTALLASJONER e Emergency shower in the vicinity e Fire extinguisher e Fire blanket e Eye washer e Chemical waste containers 126 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 318 INSTRUMENT LABORATORY Room responsible person Suthajini Yogarajah Training responsible person Supervisor ROOM ACTIVITIES e Research e Use of instruments RULES TO FOLLOW e User must keep order and clean up after themselves e Chemical spills should be removed immediately e Sign in logbok using instruments e Turn off lights when leaving room for the day Instruments and equipment Name type Responsible person Traning responsible Comments person 2 pcs Freeze Drying ins Christchurch 1 4 Suthajini Yogarajah Metanol Bath Suthajini Yogarajah NecuinpEmpe tor Suthajini Yogarajah evaporation Water distilling apparatus Suthajini Yogarajah Lab Dishwasher Suthajini Yogarajah Vacuum centrifuge Anders Asberg 4 etg RISK ANALYSIS Risk Preventive measure Incorrect use of instruments and equipment Read SOP or manual before use Always check the flasks to find an
129. tment of Pharmaceutical Biosciences BL21 2U54 FREEZING ROOM 80 CENTIGRADE Room responsible person Ragnhild Paulsen RP office no 253 tlf 44936 ROOM ACTIVITIES Sample storage at minus 80 degrees centigrade in two separate freezers RULES The door into the room must always be locked Place the samples in labelled boxes which again are stored in racks Each rack should be named Be careful not to damage the polystyrene covers The freezers alarms are for the time being not connected to the alarm central We have to be alerted 1f the alarmis activated INSTRUMENTER 2 Forma Scientific 86C Freezer ultrafreezers Responsible person Ragnhild Paulsen Training responsible person Ragnhild Paulsen 56 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 2U63A STORAGE ROOM Last update 21 06 2010 Room responsible person Ewa Jaroszewicz Training responsible person Ewa Jaroszewicz ROOM ACTIVITIES e Storage of various equipment INSTRUMENTS AND EQUIPMENT Training responsible Name type Responsible person person Notes RISK ANALYSIS Risk Precaution No risk INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e No 57 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 2U63B ATOMIC FORCE MICROSCOPY ROOM Last update 22 06 2010 Room responsible person Ewa Jaroszewicz Training responsibl
130. ts Responsible for eS Comments Training Xxxx RISK ASSESSMENT Risk Preventive measure HSE INSTALLATIONS XXXXX 147 LIST OF TELEPHONE NUMBERS AND ADDRESSES Authority 2 District Name Address Tel no E mail Web page Fire Services emergency 110 no 5 o Fire safety at UiO Technical 22 856725 brannvernOta uio no a 7 Fire safety advisor Department http www uio no for PO 1077 ansatte drift sikkerhet brannvern Blindern Ambulance emergency no 113 Oslo A amp E 22 93 2293 http www oslolegevakt no Ulleval University Switchboard 02770 Hospital 22 11 80 80 O Emergency 22 11 73 50 Z reception E ioe 53 Ophthal 22 11 8545 3 mology Dept 22 11 85 47 lt Health Services UiO 22 853174 http www sio no wps portal sio hels Emergency help e reset true Norwegian Poison 22591300 http www giftinfo no Information Centre Taxi Emergency help 22 38 80 50 Police emergency no 112 Security and alarm centre 22 85 66 66 m UiO a EMERGENCY tel O a Security and alarm centre 22855007 vaktalarm admin uio no UiO https www uio no for Non emergency messages ansatte drift sikkerhet beredskapsplan School of Pharmacy PO 1068 22 85 65 86 Director School of Blindern Pharmacy School of Pharmacy PO 1068
131. ury RESPONSIBILITY The immediate line manager at the site of the accident incident is responsible for following up the accident incident both with regard to the injured and with regard to notification on the appropriate form DEFINITION OF INJURY ACCIDENT AND NEAR ACCIDENT The form for Internal Notification of Injury must be used in the following injuries accidents and near accidents e Accidents that result in absence or medical treatment e All injuries or accidents that require first aid e All material damage and or environmental damage fire chemical spill etc e All undesired incidents dangerous conditions that could have resulted in harm to persons and or materials and the environment near accidents e All near accidents PROCEDURE 1 All personal injury accidents and near accidents are notified on the form for Internal Notification of Injury The immediate superior at the incident site is responsible for completing and distributing the form One 1 form must be used per person 2 A copy of the completed form must be sent to the Head of Department for the involved department the Director School of Pharmacy and the HSE Section PO 1071 Blindern 3 Reports are processed continuously at the School s L AMU meetings and in the department in which the injury occurred 4 The School s L AMU meetings undertake a six month audit of all notified injuries accidents and near accidents and in particular a
132. us Suthajini Yogarajah RISK ANALYSIS Risk Preventive measure Exposure of harmful toxic substances when Po Wear protective equipment Chemical spills should be removed immediately INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher 137 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 362 LABORATORY Room responsible person Training responsible person Anne Berit Samuelsen Supervisor ROOM ACTIVITIES e Research e Use of instruments RULES e The users must keep things in order and tidy up e Fume cupboards that are not used shall have their hatches closed e Switch off the light in the end of the day INSTRUMENTS AND EQUIPMENT Name type Responsible person Training responsible person Comments FPLC AKTA Kari Inngjerdigen Ingvild Austarheim Kari Inngjerdingen Ingvild Austarheim FPLC Pharmacia Anne Berit Samuelsen RI Detektor Shimadzu Ingvild Tom Erik BioRad Elektroforese Ellen H Cohen Ellen H Cohen SDS Page Ellen H Cohen Heating oven Hetosic Smi pipette Ellen H Cohen Rovapor Suthajini Yogarajah Heating block for evaporation Phenol sulfuric acid test equipment RISK ANALYSIS Risk Preventive measure Accidents and injuries due to improper use of instruments and equipment Read SOP or user s manual before use Alw
133. ust not be so full that they overflow The scales must be clean Laboratory chemicals must not be stored or used in premises designed for other use e g offices Fume cupboards must be used when working with dangerous chemicals See in addition Routines for handling and storing chemicals at the School of Pharmacy Rutine for handtering og lagring av kjemikalier ved Farmas ytisk institutt under Common routines PHARMACEUTICALS Pharmaceuticals and medicine formulations are handled in accordance with the safety datasheets for the active ingredients Narcotic substances Group A must be stored in a safe GAS CYLINDERS Gas cylinders must be securely affixed so that they cannot tip over The outside of the laboratory doors must be marked with a warning sign for gas cylinders Transport of gas cylinders must only take place after the gas regulator has been removed from the cylinder The gas cylinder is highly pressurised normal max pressure is about 200 bar which is enough to send the cylinder through the wall like a rocket if the gas cylinder falls and the gas regulator is knocked off GAS REGULATOR NB When mounting the gas regulator the threads must not be lubricated with grease as oxygen in particular can ignite the grease Be sure that all valves are switched off closed prior to opening the cylinder 26 School of Pharmacy HSE Manual Common routines Valves Gas cylinder main valve 1 Gas regulator main valve 2 Closing t
134. utoclavation of tips eppendorf tubes and the metal tubes for the Pasteur pipettes is done by the persons who have their cleaning week duty If you need training in using the autoclave ask one of the engineers at the department Equipment that is autoclaved should be placed in the cupboard in room 477 Autoclaved Pasteur pipettes should be put in the cupboard in the cell lab e Also remember to bring hazardous waste to room 025 in the basement when the box is filled up Do not leave it in the corridor in the third floor e Polystyrene boxes should be disposed of in the container at the Department of Chemistry e Glass waste is disposed of by one of the engineers when it is needed The cleaning week duties are as follows Collect the dishes in the tubs start the lab washer put the dishes on the drying racks put on tinfoil and place the dried equipment in the cupboards in room 409 The chemicals and the scales must be kept in order Autoclaving various equipment Waste disposal and making ready with new waste boxes A list with the cleaning week shift rotation should be placed in the washing room and in the cell lab The list should be signed when the work is done The tasks in the cell lab is described outside the door in the cell lab 82 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences Last template update 08 11 2010 BL25 310 NITROGEN TANK IN COLD STORAGE Room responsible person Siri Johannesen phon
135. with gas INSTRUMENTS AND EQUIPMENT Name type Responsible person Traning response Comments person Suntest chromometer H H T nnesen H H T nnesen DSC T Larsen T Larsen HPLC H Aas H Aas Risk Preventive measure Rotovapor and extruder vacuum and pressure SOP and attendance PRESSURIZED GAS See section The laboratory Safety and the working environment in this handbook to ensure correct handling of pressurized gas cylinders 46 School of Pharmacy HSE Manual Department of Pharmacy BL25186 Room responsible person Training responsible person Hoai T N Aas ROOM ACTIVITIES e Wet laboratory and monochromator room RULES e Keep chemicals and solvents in the locker e Solvents should be disposed of in the containers in the fume hood in room 160 Use the dedicated buckets for transporting solvent bottles e Howto clean the glassware Put it in a tub with diluted Deconex solution compounds insoluble in water are rinsed with alcohol Wash the glassware in the lab washer After drying the glassware should be sorted and placed in their respective drawers and cupboards Glassware should not be left on the drying rack when it s dry e Do not leave the lab without refilling commodities e Always alert the buyer in due time when commodities have to be ordred e The water supply for the water still must be turned out during the night INSTRUMENTS AND EQUIPMENT Name
136. with skin and if swallowed Very toxic by inhalation and in contact with skin Very toxic by inhalation and if swallowed Very toxic by inhalation in contact with skin and if swallowed Very toxic in contact with skin and if swallowed Irritating to eyes and respiratory system Irritating to eyes and skin Irritating to eyes respiratory system and skin Irritating to respiratory system and skin Toxic danger of very serious irreversible effects through inhalation Toxic danger of very serious irreversible effects in contact with skin Toxic danger of very serious irreversible effects if swallowed Toxic danger of very serious irreversible effects through inhalation and in contact with skin Toxic danger of very serious irreversible effects through inhalation and if swallowed Toxic danger of very serious irreversible effects in contact with skin and if swallowed Toxic danger of very serious irreversible effects through inhalation in contact with skin and if swallowed Very toxic danger of very serious irreversible effects through inhalation Very toxic danger of very serious irreversible effects in contact with skin Very toxic danger of very serious irreversible effects if swallowed Very toxic danger of very serious irreversible effects through inhalation and in contact with skin Very toxic danger of very serious irreversible effects through inhalation and if swallowed Very toxic danger of very serious irreversible effects in contact w
137. y cracks before you use the freeze dryer Always wear protective goggles Cracks in the freeze dryer flasks may lead to either explosion or implosion Closing the water supply to the water distilling Risk of water leakage apparatus outside normal working hours Do not use the water distilling apparatus and washing machine outside normal working hours INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Fire extinguisher e Fire blanket e Eye washer 127 School of Pharmacy HSE Manual Department of Pharmaceutical Chemistry BL25 330 KITCHEN OFFICE Room responsible person Engineer at the Pharmacognosy group Training responsible person ROOM ACTIVITIES e Simple food making e Copying scanning RULES KITCHEN Everyone is obliged to keep things in order in the kitchen If you store food in the kitchen take care to remove it before it gets poor After making sandwiches or any other food you have to tidy Dirty dishes have to be put in the dishwasher If there is a posting with the word WASHED you may put the cleaned dishes in the kitchen cupboard If the diswasher is full of dirty dishes you may add detergent and start the dishwasher If you don t know how to do this ask the room responsible person There are some postings in Norwegian at the kitchen s cupboard doors RULES OFFICE INSTRUMENTS AND EQUIPMENT Name type Responsible person oe
138. y equipment when handling chemicals Work in fume hood Clean up spillage of chemicals immediately INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e No 66 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 1U67 MEDIUM CULTIVATION ROOM Room responsible person Mona Gaarder Training responsible person Mona Gaarder ROOM ACTIVITIES e Lab couse preparation e Manufacturing of cultivation dishes e Preparations before autoclavation INSTRUMENTER UTSTYR Name type Responsible person Training responsible person Notes Disc distributing machine Mona Gaarder Mona Gaarder Dry sterilization unit Mona Gaarder Mona Gaarder Water bath Mona Gaarder Mona Gaarder Micro wavw oven Mona Gaarder Beckman spectophotometer Mona Gaarder Mona Gaarder Genius PCR machine Mona Gaarder RISK ANALYSIS Risk Preventive measure None INSTALLATIONS REGARDING HEALTH ENVIRONMENT AND SECURITY e Eye washer e Fire extinguisher 67 School of Pharmacy HSE Manual Department of Pharmaceutical Biosciences BL21 1U69 AUTOCLAVE CLEANING ROOM Room responsible person Mona Gaarder Training responsible person Mona Gaarder ROOM ACTIVITIES e Cleaning equipment in the lab washer e Autoclavation of equipment fluids and bacterial waste INSTRUMENTS AND EQUIPMENT Name type Responsible person ei
139. y to incineration 21 School of Pharmacy HSE Manual Common routines 5 7 OTHER CHEMICAL REMNANTS DELIVERY TO WASTE COLLECTION CENTRES 5 7 1 NB Explosive self igniting infection hazard radioactive substances or organic peroxides are not accepted This type of waste must be handled individually and separately 5 7 2 Packing and sorting Sorting must first be undertaken by the person responsible for the Chemicals routine at the School of Pharmacy as stipulated on the NORSAS forms The form and guidelines must be ordered from NORSAS AS see www norsas no The following are the most used substance categories with corresponding EAL codes at the School of Pharmacy Substance Waste category EAL code no 7041 Organic solvents halogenated 140602 7042 Organic solvents non halogenated 140603 7043 Paints adhesives varnishes solvent based 080111 7053 Paints adhesives varnishes water based 080111 7055 Pressurised cans 160504 7081 Mercury containing waste 060404 7083 Cadmium containing waste 060405 7091 Inorganic salts containing heavy metals 060313 7092 Lead accumulators 160601 7122 Highly reactive substances 160506 7123 Organic peroxides 160508 7131 Acids inorganic 200114 7132 Bases inorganic 200115 7133 Cleaning agents 200129 7134 Acidic organic waste 160508 7151 Organic waste halogenated 160508 7152 Organic waste non halogenated 160508 7220 Photo

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