Rainin Classic™
Contents
1. PR 5000 PR 10ML do not use tip ejectors Volume Indicator The volume indicator is read from top to bottom Up to PR 200 black digits indicate microliters and red digits tenths and hun dredths of microliters For PR 1000 and PR 5000 red digits indi cate milliliters and black digits microliters For PR 1OML black digits indicate milliliters and red digit tenths of a milliliter PR 2 PR 10 PR 20 PR 100 PR 200 PR 1000 PR 5000 PR 10ML 1 0 1 0 1 0 1 0 1 4 2 7 2 7 2 7 2 7 Black Digits Red Digits 5 5 5 5 5 5 5 5 125uL 7 5pl 12 5pL 75pL 125uL 075mL 125mL 75mL Sample values volume ranges and smallest increments for each Rainin Classic model are shown below Range Smallest Model Adjustable Recommended Increment pL PR 2 0102 0 1102 0 002 PR 10 01010 0 5 to 10 0 02 PR 20 0 to 20 2 to 20 0 02 PR 100 0 to 100 10 to 100 0 2 PR 200 0 to 200 20 to 200 0 2 PR 1000 0 to 1 000 100 to 1 000 2 0 PR 5000 0 to 5 000 500 to 5 000 2 0 PR 10ML Oto 10 mL 1 mL 10 mL 20 0 Safety Filter PR 5000 and PR 10ML Models PR 5000 and PR 10ML use a safety filter in the shaft to help prevent liquid entering the shaft and contacting the piston if the plunger snaps up during aspiration This is particularly important using large volumes If the filter gets wet replace it For PR 5000 insert the small diameter into the shaft for PR 10ML insert the large diamet2. Return excess sample in the tip to the original sample con tainer if desired Discard the used tip Pipetting Liquids of Varying Density Rainin Classic lets you compensate for solutions of density much different from water by setting the volume slightly higher or lower than that required The compensation amount must be determined empirically E g if pipetting pL of CsCl solution you determine that the volume delivered is actually 8 5 uL average of 5 samples Try increasing the volume setting to 11 8 uL and repeat the measure ments If the volumes delivered are still not close enough to uL make another slight volume adjustment until the measure ments are as desired Very dense liquids may not be suitable for air displacement pipet ling Use Rainin Pos D positive displacement pipettes for these liquids Temperature Considerations Warm or cold liquids can be measured with good precision by using a consistent pipetting rhythm This will help minimize any differences in heating or cooling effects within the pipette Use a new tip each time for best accuracy and precision when measur ing samples with temperatures greatly different from ambient and do not pre rinse You will get best results if there is no delay between picking up the sample and dispensing it If working in a cold room allow the pipette to stablize at ambient temperature before operation Acids and Corrosives After pipetting concentrated acids
3. above mentioned facilities For pipettes under warranty please note that the warranty will be voided if the pipette has been damaged as a result of physical or chemical abuse or if the pipette has been repaired or recalibrated by any service facility which is not authorized by Rainin Contact Technical Support at 800 543 4030 for further information Removing Replacing Tip Ejector Arm 1 Fully depress the Tip Ejector Button I E d 2 Press in the Quick Release Tabs and pull the Tip Ejector Arm off Figure 3 Removing the Tip Ejector Arm Removing the Tip Ejector Arm 1 2 Press and hold the tip ejector button fully down With the other hand press in the quick release tabs on the tip ejector arm and pull the tip ejector arm straight down Replacing the Tip Ejector Arm 1 2 Press and hold the tip ejector button fully down Hold Rainin Classic in one hand and the tip ejector arm in the other hand Insert the end of the shaft through the large opening in the tip ejector arm Align the top end of the tip ejector arm with the pushrod inside the handle and push the tip ejector arm into the han dle until the tip ejector arm snaps in place Make sure that tips fit securely on the shaft Performance Specifications Each Rainin Classic is factory calibrated and carefully checked gravimetrically before shipment using distilled water and an ana lytical balance Water temperature
4. and ambient conditions are stabilized at 21 5 C 1 C Volumetric corrections are made for both the density of water and evaporation where applicable Consult the Rainin publication Procedure for Evaluating Pipette Accuracy and Precision AB 15 for further information or down load a copy from the RAININ website http www rainin com pdf ab 1 5 pdf When used in accordance with the pipetting procedures in this manual and with Rainin tips Rainin Classic pipettes will perform fo the following specifications These manufacturer s specifications should be used as guide lines when establishing your own performance specification Volume Increment Accuracy Precision ul L ul uL 0 2 12 0 024 6 0 012 1 0 2 7 0 027 1 3 0 013 2 0 1 5 0 030 0 7 0 014 1 2 5 0 025 1 2 0 012 5 j 1 5 0 075 0 6 0 03 10 1 0 1 0 4 0 04 2 7 5 0 15 2 0 04 5 3 0 15 0 9 0 045 10 1 5 0 15 0 5 0 05 20 1 0 2 0 3 0 06 10 3 5 0 35 1 0 1 50 E 0 8 0 4 0 24 0 12 0 8 0 8 0 15 20 2 5 0 5 1 0 0 2 0 8 0 8 0 25 0 8 1 6 3 3 0 6 0 8 4 0 2 0 8 8 2 4 12 0 6 0 6 15 0 2 0 6 30 5 50 0 6 1 50 0 2 0 6 60 Specifications are subject to change without notice gt cT O nmuo e T Replacement Parts Part Model PR 2 PR 1O PR 20 100 PR 200 PR 1000 PR 5000 PR lOML Plunger button 6191 329 6191 330 6191 331 6191 332 6191 333 6191 334 6191 335 6191 3
5. 3 Fax 858 320 0556 Massachusetts Rainin Road Woburn MA 01801 Tel 800 662 7027 Fax 781 935 7631 Canada 6419 Northam Drive Mississauga ONT L4V 1J2 Tel 866 823 4022 Fax 866 758 3991 Japan 4 1 11 Bunkyo Ku Tokyo 113 0033 Tel 03 5689 8311 Fax 03 5689 2670 METTLER TOLEDO Pipette Repair and Calibration facilities Belgium N V Mettler Toledo s a B 1932 Zaventem Tel 02 334 02 11 Fax 02 334 03 34 Germany Mettler Toledo GmbH D 35353 Giessen Tel 0641 50 70 Fax 0641 507 128 Denmark Mettler Toledo A S DK 2600 Glostrup Tel 43 270 800 Fax 43 270 828 Spain Mettler Toledo S A E E 08038 Barcelona Tel 93 223 76 00 Fax 93 223 02 71 France HTS F 28000 Chartres Tel 02 37 88 31 00 Fax 02 37 88 31 09 Italy Mettler Toledo S p A 1 20026 Novate Milanese Tel 02 333 321 Fax 02 356 29 73 Netherlands Mettler Toledo B V NL 4004 JK Tiel Tel 0344 63 83 63 Fax 0344 63 83 90 Sweden Mettler Toledo AB S 12008 Stockholm Tel 08 702 50 00 Fax 08 642 45 62 Service is also available in many other countries through autho rized RAININ distributors See www rainin global com It is recommended to use only genuine RAININ replacement parts such as seals and shafts It is NOT necessary to recalibrate the pipette after changing the seal or shaft Recalibration of the pipette is only necessary when the piston is replaced and should be done only by qualified factory trained personnel in one of the
6. 36 Plunger rod 6191 198 6191198 6191 198 6191 198 6191 198 6191 198 6191 198 6191 198 91 295 6191 295 6191 295 6191 295 6191 295 6191 295 2 2 Shaft coupling 6 Shaft 44816 44819 23353 44602 23305 123371 6191 355 6192 016 Tip ejector 6191 196 6191 196 6191 197 10200 148 6200 1566200 163 n a na O ring 400071 400071 400013 400067 400001 400003 400006 6192 015 Seal 44815 44818 6191 298 6191 299 6191 300 6191 301 302 n a o Seal assy 44817 44817 23354 44603 123300 t t t holder Small spring 6191 303 6191 303 6191 303 6191 303 6191 303 Small spring 2387 23871 23871 2387 23871 t i t positioner Lorge spring 44214 44214 44214 44214 44214 t positioner Large spring 6191 304 6191 304 6191 304 6191 305 6191 305 t T Filters 100 n a n a n a 6190 164 6190 164 Filters 1000 n a n d n d na 6190 165 6190 165 Part of one piece piston assembly Replacement will require recalibration Rainin is the only organization authorized to calibrate Rainin Classic pipettes Please call 800 662 7027 for information See replacement parts diagrams on page 12 and 13 Accessories Rainin Classic is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation Sev
7. 81 3 5689 8311 Fax 81 3 5689 2670 METTLER TOLEDO GmbH Switzerland Phone 41 1 944 45 45 Fax 41 1 944 45 10 Other International Offices and Distributors See www rainin global com E mail global amp rainin com from outside the U S Web www rainin global com from outside the U S RAININ Rainin Instrument LLC 7500 Edgewater Drive Box 2160 Oakland CA 94621 0060 Prices and specifications are subject to change without notice Copyright 2005 Rainin Instrument LLC a METTLER TOLEDO Company 9920 332 Rev C
8. Rainin Classic continuously adjustable digital microliter pipettes 8 models for volume ranges from 0 1 pL to 10 mL PR 2 PR 10 PR 20 PR 100 PR 200 PR 1000 PR 5000 PR 10ML Made in the USA by Rainin Instrument LLC Rainin Classic PR 200 shown RAININ Rainin Classic continuously adjustable digital pipettes CONTENTS DOSCIIPT OM ies etse teretes i eirca 1 o eo roe vero editor Ads ns 2 AUTOGIOVING cett daie ret Pee eed na 2 Vol me IndiCatOr aii pete e 2 Safety Filter 5000 2 e gae ratu ter eter or 3 TIP SEIEN i i eoe tote bes e ec erem 4 Tip Immersion Depth eb b cete 4 Pipetting Guidelines amp 5 Pre Rinsing Recommended 5 Reverse Mode Pipeltting eeenee 5 Pipetting Liquids of Varying 6 Temperature Considerations 6 Acids and 6 MIOLOGC ood ee 7 Troubleshooting and 7 Sample Splash ee 7 Leaks Inaccurate Sampling 7 Service Calibr
9. and reassemble after inspecting the interior of the shaft for contaminants 4 If staining and or corrosion of the piston is evident do not use the pipette Return to Rainin for service see p 8 Never grease any Rainin Classic components The only exception Model PR 10ML uses a grease seal Leaks Inaccurate Sampling 1 Loosened shaft Tighten coupling by hand 2 Split or cracked shaft Remove the tip ejector and inspect the shaft for fracture or split end Replace if necessary If the shaft was dropped remove it and the seal assembly to see if the piston is bent If so you should return the instrument call 800 543 4030 for assistance 3 Worn seal and or o ring Dismantle as described in Sam ple Splash Replace the seal and o ring referring to the drawing on page 12 or 13 All models except PR 10ML use a polyethylene teflon seal and o ring Pull off the old seal and o ring position the new seal and o ring on the pis ton as shown the drawing and reassemble the pipette 4 Bent plunger damaged friction ring Call 800 543 4030 5 Improper reassembly Remove the tip ejector and shaft Check the position of the internal assemblies especially the seal against the illustrations Service Calibration and Repair RAININ Pipette Repair and Calibration facilities California 7500 Edgewater Drive Oakland CA 94621 Tel 800 662 7027 Fax 510 564 1683 5955 Mira Mesa Blvd Ste B San Diego CA 92121 Tel 858 320 044
10. ation and 8 Removing Replacing Ejector Arm 9 Performance Specifications 10 Replacement Parts 11 FIGURES Figure 1 Rainin Classic 200 1 Figure 2 Operating the Classic 3 Figure 3 Removing Tip Ejector 9 DIAGRAMS FOR REPLACEMENT PARTS Models PR 2 PR 10 PR 20 100 12 Models PR 200 PR 1000 PR 5000 PR 10ML 13 TABLES SpeGITICOTIOnS sc UL Pha DE eT AM age Maso 10 Technical Assistance 800 543 4030 Call this toll free number for technical consultation and product information for Rainin Classic other Rainin pipettes and dispos able tips E mail tech service rainin com Rainin Classic is made in the USA by Rainin Instrument LLC Copyright 2006 Rainin Instrument LLC Rainin and Rainin Classic are trademarks of Rainin Instrument LLC Rainin Classic pipettes are manufactured under U S Patent 5 614 153 Prices and specifications are subject to change without notice Description Eight models of the Rainin Classic continuously adjustable digital pipette cover the entire volume range from 0 1 uL to 10 mL Rainin Classic pipettes are not limited to fixed
11. er into the shaft Part numbers 6190 164 pack of 100 and 6190 165 pack of 1000 END OF END OF 5000 pL SHAFT 10 mL SHAFT M Operation 1 Turn the plunger button or the volume adjustment knob until the volume indicator is revolution above the desired set ting then turn slowly clockwise until the desired volume shows on the indicator ALWAYS dial down to the desired volume This prevents mechanical backlash from affecting accuracy If you pass the desired setting turn the dial 1 3 revolution higher than desired and dial down to reset the volume The friction ring prevents unintentional volume changes Attach a new disposable tip to the pipette shaft Press into the tip with only enough force to make a positive airtight seal Press the plunger fo the First stop This part of the stroke is the volume displayed on the indicator Holding Rainin Classic vertically immerse the tip into the sample to the proper depth see table on page 4 Allow the pushbutton to return slowly to the up position Never let it snap up See Figure 2A below Pause briefly to ensure that the full volume of sample is drawn into the tip Withdraw the tip from the sample liquid If any liquid remains on the outside of the tip wipe it carefully with a lint free tissue avoiding the tip orifice To dispense sample touch the tip end against the side wall of the receiving vessel and depres
12. eral hangers are available to hold Rainin Classic in a clean safe place when not in use Fy zm HU M3 Set of magnetic HU S3 3 Hang Ups attached Hang Ups for mounting on fo a clamp which fits onto a ferrous surfaces Includes shelf adhesive disks i CR 7 Free standing carousel GR 3 Clear stand holds 3 holds 7 Rainin pipettes Rainin pipettes 10 100 PR PR Part numbers are listed on page 11 12 PR 1000 PR 10ML PR 200 PR 5000 13 Limited Warranty See the enclosed Limited Warranty and Limitations of Liability Statement Please complete and return the Warranty Registration Card on receipt of your pipette RAININ pipettes are calibrated with RAININ tips To assure excel lent reproducibility and performance use only RAININ tips as rec ommended in this manual Specified performance is guaranteed only when RAININ tips are used Contacting RAININ Direct Order Line Phone 800 472 4646 Fax 510 564 1617 E mail pipets rainin com Technical Information Phone 800 543 4030 Fax 510 564 1617 E mail tech supportGrainin com Pipette Service Phone 800 662 7027 Fax 510 564 1683 E mail service rainin com Web www rainin com online ordering 24 7 From outside the U S Main Office 001 510 564 1600 RAININ Japan Phone
13. or highly corrosive solutions you should disassemble Rainin Classic and inspect and clean if nec essary the piston shaft and seal assemblies Extensive contact with corrosive fumes may corrode the piston This will result in premature seal wear and may require refinishing or replacement of the piston Exposure of internal components to corrosive fumes can be reduced by using aerosol resistant tips These tips have an internal filter which acts as an aerosol barrier Storage Rainin Classic is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation Several hangers are available to hold Rainin Classic securely when not in use shown in the Accessories Section page 11 HU M3 Magnetic Hang Ups 53 Shelf Hang Ups CR 7 Carousel GR 3 Clear Pipet Stand Troubleshooting and Repairs Rainin Classic pipettes give excellent performance and long term service Use these procedures in the case of physical or chemical damage Note that PR 2 and PR 10 models have small fragile components that can be broken or misplaced Sample Splash liquid inside the mechanism 1 Remove the tip ejector if fitted see page 9 2 Unscrew the shaft coupling nut holding the shaft to the body 3 Remove the shaft and inspect the seal assembly and piston for contamination The piston should be shiny and free of corrosion Clean with distilled water or isopropyl alcohol Dry with a lint free tissue
14. s the plunger slowly to the first stop See Figure 2B TOP OF STROKE 1 1f 2ND STOP 7 Figure 2 Operating Rainin Classic Wait 1 second PR 2 PR 10 PR 20 PR 100 PR 200 1 2 secs PR 1000 2 3 secs PR 5000 PR T1OML Longer for viscous solutions Then press the plunger to the secon stop bottom of stroke expelling any residual liquid in the tip 10 With the plunger fully pressed withdraw the pipette from the vessel carefully with the tip against the vessel wall 11 Allow the plunger to return to the up position 12 Discard the tip by depressing the tip ejector button A fresh tip should be used for each sample fo prevent sample carryover Tip Selection m Tips must seal properly on the shaft to assure an air tight seal and avoid leaks or poor accuracy m Tips must be soft and flexible so that the shaft is not scratched or worn prematurely m Tips must be free from microscopic flash and particulates Flash gives poor precision and accuracy m The tip orifice must be the correct size and orifice size and geometry must be consistent from tip to tip Otherwise accura cy and precision will be affected m Interior and exterior surfaces must be clear smooth and hydrophobic to avoid retention of liquid Too much retention results in poor accuracy and reproducibility Specified performance is guaranteed only when Rainin dispos able tips are used as recommended in this man
15. slowly holding the pipette 20 from vertical For PR 5000 and PR 10ML always use the special safety filters supplied Part numbers 6190 164 pack of 100 and 6190 165 pack of 1000 Pre Rinsing Recommended Some solutions e g serum protein containing solutions and organic solvents can leave a film on the inside tip wall resulting in an error larger than the tolerance specified Since this film remains relatively constant in successive pipettings with the same tip excellent precision may be obtained by refilling the tip and using the refilled volume as the sample Successive samples from this same tip will exhibit good reproducibility low variance Reverse Mode Pipetting Another way of reducing error due to film retention is reverse mode pipetting the operating sequence is reversed 1 Mount a disposable tip on the pipette shaft 2 Press the pushbutton fully to the SECOND STOP 3 Immerse the tip in liquid and return the plunger slowly to the full up position Wait a moment for the liquid column to reach equilibrium in the tip 4 Wipe any excess liquid from the outside of the tip without touching the orifice 5 To dispense rest the end of the tip against the vessel wall and press the plunger to the First stop only Hold at the First STOP for a few seconds long enough for the liquid column to reach equilibrium again 6 Remove the tip from the receiving vessel without blowing out the remaining liquid 7
16. ual Rainin cannot accept responsibility for poor performance resulting from the use of tips by other manufacturers Rainin tips are mold ed from premium grade virgin polypropylene plastic Samples from each lot of tips are inspected microscopically to ensure that every lot meets Rainin s high standards Tip Immersion Depth The recommended depth for tip insertion into the sample for each Rainin Classic model is shown in the table below Model Immersion Depth 2 10uL 1 2 20 100 uL 2 3mm 200 1000 pL 3 6mm 5000 uL 10 mL 6 10 mm Tip immersion depth is important If exceeded the volume measured will be inaccurate possibly out of specification Tip angle is also important Hold the pipette vertically or within 20 degrees of vertical 4 Pipetting Guidelines amp Precautions Consistency in all aspects of pipetting procedure will significantly contribute to optimum reproducibility Use a 1 Consistent pickup dispense rhythm while pipetting 2 Consistent speed and smoothness when you press and release the pushbutton 3 Consistent pushbutton pressure at the first stop 4 Consistent immersion depth 5 Minimal angle lt 20 from vertical Prevent liquids from being drawn into the shaft by taking the fol lowing precautions 1 Use Rainin aerosol resistant tips with an internal filter which acts as a barrier to aerosols and liquids Never invert or lay the pipette down if liquid is in the tip Pipette
17. volume increments e g 5 uL or 10 uL They can be set to any volume in range e g 6 6 133 3 377 2228 Piston stroke is set by adjusting a micrometer coupled directly to a digital volume indicator which reads in microliters The digital volume indicator simplifies volume setting and virtually eliminates calculation errors All Rainin Classic models have a highly polished stainless steel piston polyethylene teflon seal and o ring except PR 10ML which uses a grease seal Rainin Classic requires no routine lubrication and will give years of trouble free service A stainless steel tip ejector is provided with all models except PR 5000 and PR 10ML for safe disposal of used tips The ejec tor has a quick release mechanism see page 9 Plunger button volume adjustment Fingerhook N A Tip ejector button Friction ring except PR 5000 amp volume adjustment PR 10ML Precision micrometer B High impact Digital volume low thermal indicator conductivity body Ejector arm quick release Shaft Stainless steel tip ejector arm not present on PR 5000 PR 1OML Polypropylene disposable tip Figure 1 Rainin Classic PR 200 1 Contents Rainin Classic as ordered Sample tips Instruction Manual Rainin Test Report Performance Assurance Brochure If any item is missing call Customer Service 800 472 4646 Autoclaving The shaft and tip ejector are autoclavable 121 C 1 atm 15 20 minutes
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