Richter Optica U2 Microscope User`s Manual
Contents
1. Avoid placing the instrument in locations exposed to direct sunlight dust vibration high humidity and where it is difficult to unplug the power supply cord ASSEMBLING THE MICROSCOPE 1 Objectives Lower the stage completely 1 Screw the objectives into the revolving turret nosepiece 2 so that each clockwise rotation brings the next highest magnification objective into position 2 Condenser Raise the stage by turning the coarse focus knob 1 Raise the condenser carrier by turning the condenser focus knob 2 Insert the condenser into the mount with aperture scale facing forward towards the user Secure with the condenser clamp screw 3 Turn the condenser focus knob to raise the condenser as far as it will go 3 Eyepieces Use the same magnification eyepieces for both eyetubes To lock the eyepiece insert each eyepiece into the eyetube completely and tighten the small clamping screws 4 Power Cord p Connect the socket of the power cord 1 into the AC inlet on the rear of the base of the microscope 2 Plug in the other end of the cord to an AC AG outlet with ground conductor 5 RICHTER Eer ca MICROSCOPY 1 Coarse and Fine Focusing Focusing is done with coarse and fine focus knobs at the left and right of the microscope stand The direction of vertical movement of the stage corresponds to the direction the focus knobs are turned Never attempt either of th2. RICHTER PTICA Richter Optica info richter optica com Instructions for Models U2B U2T U2 LCD U2D University Laboratory Biological Microscope RICHTER Eer ca Trinocular Port Interpupillary Adjustment Scale Eyepieces Diopter Adjustment Ring Binocular Eyepiece Tube Revolving Nosepiece Specimen Holder Objective Stage Condenser Stage Y Axis Focusing Knob Travel Knob Stage X Axis Travel Knob Fine Focus Knob Lamp Housing Coarse Focus Knob Illumination on off switch Light Intensity Control RICHTER pprica Thank you for your purchase of a Richter Optica microscope The information in this manual is provided to answer most questions that can arise when operating your microscope and to help you avoid unneccesary maintenance expenses in the future Please carefully read instructions before operating microscope Nomenclature used to describe components and controls are identified on opposite page UNPACKING Do not discard styrofoam container or packing materials until you are sure shipment is complete and undamaged retain styrofoam shipping container to store your microscope when it is not in use Remove all tape and packing material used to protect microscope during shipment Make certain lens surfaces do not come in contact with dirt fingerprints or oil Damage of lens surfaces occur when they come in contact with such contaminants and image quality is reduced SETTING UP THE INSTRUMENT
3. ake sure the microscope is off and unplugged Remove the power cord Remove the fuse Zag groups 1 from the microscope oz base 2 by inserting a coin or N flathead screwdriver and twisting counter clockwise Install anew fuse and replace fuse groups Fuse 250V 3 15A es A gags RE za EWE 4 en j DAD RICHTER rrica TROUBLESHOOTING Optical Possible Cause Dust or dirt in field of view Dust or dirt on objective filter condenser or eyepiece Poor image low contrast or resolution Condenser is set too low or too high Aperture diaphragm closed too far Incorrect illumination Uneven Focus Specimen holder not fixed securely on stage Specimen not secured in position Specimen tilted on stage Image tinged yellow Lamp voltage is set too low Blue filter is not being used Focusing is not possible with high magnification objectives Slide is upside down Cover glass is too thick Eyepiece diopter not adjusted Insufficient pafocality of objectives Eyepiece diopter not adjusted Magnification or field of view of left and righ eyepiece No cohesion of binocular image differ Interpupillary distance not adjusted Eyepiece diopter not adjusted RICHTER rrica Optical Possible Cause High magnification objectives strike the specimen when changing over from low to high magnification Slide is upside down Cover glass is too thick Insuf
4. and off 5 For stubborn dirt dampen a piece of gauze with diluted neutral detergent and wipe lightly Disinfecting the Microscope Follow the standard procedures for your laboratory When notin Use 1 When not in use cover instrument with dust cover and store in a place in low humidity where mold is not likely to form 2 Proper handling of the microscope will ensure years of trouble free service 3 If repair becomes necessary please contact Richter Optica directly at info richter optica com RICHTER rrica Uy o Evepicee pe FS uf OF am Chi scive ay ana cD Quick Assembly Guide 1 Install condenser 2 Screw in objective lenses into turret nosepiece 3 Insert and secure eyepieces 4 Connect power cord Power Cod
5. e following actions since doing so will damage the focus mechanism Rotate the left or right knob while holding the other stationary Turning the coarse and fine focus knobs further than their limit 2 Coarse Focus Tension Adjustment To increase the tension turn the tension adjustment ring located on the inside of the coarse focus knob in the direction indicated by the arrow 3 Interpupillary Distance Adjustment Before adjusting the interpupillary distance bring a specimen into focus using the 4x objective While looking through the eyepieces move the eyetubes either further apart or closer together until the right and left field of view become one On either side of the scale 2 line up the dots 1 This adjustment will enable the user to see the specimen with both eyes 4 Diopter adjustment Diopter adjustment will compensate for the differences in vision between the left and right eyes In addition to making observation with both eyes easier it will also help reduce the extent to which focusing is lost when the objective magnification is changed In particular this occurs when a low magnification objective is used Rotate the nosepiece until the 4x objective clicks into position Using the fine and or coarse focus knobs focus the microscope until the image is at its sharpest The left and right eyepieces have separate focusing provisions to compensate for slight differences in the focusing of each eye Using t
6. ficient parfocality of objectives Eyepiece diopter not adjusted Interpupillary distance not adjusted Eye strain or fatigue Diopter adjustment not made Field of view of left and right eyepiece differ Inadequate illumination Electrical Lamp does not light Power supply not plugged in Lamp not installed Lamp burned out Inadequate brightness Specified lamp not being used Lamp blows out immediately Specified Lamp not being used Lamp flickers Connectors are not securely connected Lamp near end of service life Lamp not securely plugged into socket CARE AND MAINTENANCE Do not disassemble 1 Disassembly may significantly affect the performance of the instrument and may result in electric shock or injury and will void the terms of the warranty 2 Never attempt to dismantle any parts other than described in this manual If you notice any malfunction contact your Richter Optica representative Cleaning the microscope 1 Do not use organic solvents such as ether alcohol or paint thinner on painted surfaces or plastic components Doing so could result in discoloration of painted or plastic surfaces 2 When cleaning lenses do not use any solvents other than absolute alcohol as they may damage lens bonding cement 3 Do not use petroleum benzene when cleaning components such as filters or lenses 4 Absolute alcohol and petroleum benzene are highly flammable Keep away from open flames and when turning power switch on
7. he right eye only and viewing through the right hand eyepiece adjust the diopter until the image is clearly in focus Using the left eye only and viewing through the left hand eyepiece with its independent diopter focusing ring focus until the specimen is at its sharpest The microscope should now be ready for binocular viewing When switching between users note the setting on the scale 2 on the eyepieces and you can quickly change the proper setting back for each user RICHTER rrica MICROSCOPY 5 Adjust Light Intensity When the power switch is turned on adjust the light adjustment knob 1 on the side of the microscope to increase the voltage and brightness 6 Specimen Holder Push the finger lever 1 on the specimen holder back to place the slide 2 on the mechanical stage Control the X Y axis with the knobs on the right side of the microscope 3 to move the sample left to right and forward and backward on the stage 7 Adjust the Iris Diaphragm Images will have the best contrast and resolution when the numerical aperture NA of the iris diaphragm is set to match the NA of the objec tive lens being used Move the lever 3 on the iris diaphragm so that it lines up with the corresponding objective setting 4 8 Using the Filter Place the filter over the illuminator see image at left to increase contrast or change the background lighting of your microscopy image 9 Replacing the Fuse M
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