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1. 3 z a as Click on the button Acquiring the map image Double click on the position container that you want to acquire the map image Click on the button to start the image acquisition 6 When the map image is completed click on the PWA button Now the map image has been acquired 4 Image Acquisition Selecting the acquisition mode Click on the J button to select the z Z Stack i B tion acquisition mode Double click on the observation target position container that you want to acquire the image Click on the ING navigation button to view the Navigation The following five acquisition modes are available Time Lapse Sequential acquisition of the images of a specified area at the specified time intervals Z Stack Sequential acquisition of the images of a specified area by varying the focused position at constant steps Z Stack Time Lapse Sequential acquisition combining the Z Stack and Time Lapse modes Multi Area Time Lapse Time Lapse acquisition while moving across multiple specified areas Multi Area Z Stack Time Lapse Z Stack Time Lapse combined acquisition while moving across multiple specified areas Hereafter continue operation by following the instructions given by the Navigation Please choose the image size to observe When the operation is completed click on the button to view the next operated position and the instruction on it2. OFF Press and hold the sub switch on the main unit for 3 seconds to turn it off Set the main switch on the power supply unit to O OFF as required F LUOVIEW FVIOia 66666666666666 15 Printed in Japan on February 00 2010 M 000 01
3. specimen holder Press and hold the Water botton It may sometimes take as long as 30 seconds to 1 minute until Please keep the button pressed until water comes out the water has reached the nozzle When water comes out of the water feed nozzle release the Water botton Install the specimen holder in the original position 6 Close the cover From the Setup Dyes list select the z fluorescent dyes for use in observation ff and click on the Apply gt button Up to 4 dyes can be selected oj E PPE MII TEU param zza koza ur a a An marza pz TLD ALLELE TTT Ii junk TUNAN KRAKET papery Click on the gt START button zem UJ it Ty et anana Fae Th PETERA UNA int a1 Now the software setup is complete uononponulj SIOJ U07 UIEW MO 4 uolisinboy uoneAlesqO Observation Acquisition Flow Introduction Main Controls 3 Map Image Acquisition The map image is the overall view of the specimen and used to locate the observation target The description in this manual takes an example in which a fluorescence image is selected and acquired as the map image Setting the map image acquisition condition Select Use Fluorescence ED 09 anana ne na AN a When more than 3 kinds of fluorescent 2 w ZA dyes have been chosen select the wa combination of fluorescent dyes to be Seren used in the map image acquisition
4. 6 Push and hold the cover lock buttons on both sides and lift the cover ym Install the specimen holder carrying the specimen to be observed in the main unit For details refer to the User s Manual Hardware FLUOUIEW EUlo The software cannot be launched unless the specimen holder is installed 8 Push down the cover until it clicks to indicate that it is closed Observation is not available unless the cover is closed completely Stable images can be acquired if acquisition is started after the following warm up period FV10i W 60 minutes FV10i O 30 minutes Now the system setup is complete uononponulj S OJ U07 UIEN MO 4 uolisinboy uoneAlesqO Observation Acquisition Flow Introduction Main Controls 2 Software Setup Launching the software Wait until the left STATUS LED on the main 4 AN unit changes from blinking to steady gt N lighting Double click on the FV10i SW icon on the desktop to launch the software Welcome to FV10 ASW After the splash screen the logon OLYMPUS screen appears on the monitor screen Log on with your own user ID User ID Administrator If you have not yet registered your user NAN ID log on with the factory default ID Factory default ID Administrator Password Administrator The operation preparation screen appears Checking water in the water feed nozzle FV10i W only Open the cover Remove the
5. Note This manual introduces a simplified operating procedure of the product so that even the person who uses it for the first time can complete the operations For detailed operating procedures refer to the separate User s Manuals Safety and Hardware as well as the Navigation Online Help of the software Retain this manual in an easily accessible place near the work desk for future reference AX7923 TYPE 2 Introduction Main Controls Observation Acquisition Flow AN SAFETY PRECAUTIONS During observation and acquisition the cover is kept closed to avoid the risk on the user However when replacing the specimen holder the cover should be opened so care is required in this operation Also note that the laser beam is screened by a shutter and therefore safe while the cover is opened g A A a a iia FV10i W only FLUOVIE This section becomes hot for keeping the incubator temperature Caution against finger being caught This section accommodates motorized modules and the unit should not be operated while the cover is open Also be careful not to have your hand or finger caught by the cover when closing it Caution for specimen replacement Always remove the specimen together with the specimen holder Observation Methods Fluorescence observation Phase contrast observation This method utilizes the fluorescence This method makes it possible to observe a generated when the specimen is i
6. on Main Controls This section describes the basic operation flow in sequence together with the monitor screen displays System Setup Software Map Image Setup Acquisition gt bp 6 gt bp 8 gt p 10 Image Acquisition gt p 11 LJ 4 A a 2 a Ba t a R CAUTION The description in this section assumes that the following preparation work has been completed e he cables are connected properly e he tubes are connected FV10i W only For the connection methods refer to the separate User s Manual Hardware FLUO ena SW Image Editing System Termination Analysis gt p 13 gt p 14 uononponulj SIOJ U07 ulEW MO 4 uonis inboy uonessqo Observation Acquisition Flow Introduction Main Controls 1 System Setup Starting the system Set the main switch on the power supply unit to ON Set the sub switch on the main unit to turn it on To start up the main unit without turning the heater ON press and hold the sub switch until a short beep is generated for about 3 seconds Set the main switch of the display to ON Open the front cover of the controller and set the main switch to ON Log on Windows Vista with your own user ID If you have not yet registered your user ID log on with the factory default ID Factory default ID fluoview Password fluoview Installing the specimen holder
7. rradiated non stained specimen living specimen or with strong light to observe the specimen nearly transparent specimen which is hard structure or identify the substance according to observe in brightfield observation by to the presence absence and color tone of enhancing the contrast between brightness the fluorescence and darkness Nomenclature Incubator temperature indicator FV1i0i w only The indicator blinks during warm up and light steadily when the optimum temperature 34 to 38 C is reached The temperature indicator shows when the heater is OFF Cover lock buttons Provided on both sides Push and hold the bottoms on the left and right sides to open the cover Cover FV10i W main unit FV10C W2 FV10i O main unit i Q FV10C 02 STATUS LEDS mg Sub switch When both the main and sub switches are ON LEDs p 4 Left Right Laser Software Malin switch ZE5 Power Supply Unit FV10C PSU NG BG Initializing ON Blinking Lighting OFF e Not run Lighting Lighting 4m Standby 4 Running Lighting Blinking ZO S 4 i gus Both the left LED and right LED blink when the cover is open The operations other than those available with the above controls can be adjusted or set from the software The operations are easy using the Navigation gt p 11 uononponul MO uonis Inboy uonessqo S OJJUOD UIEW Observation Acquisition Flow Introducti
8. uononponul SIOJ U07 ulEW MO 4 uonis inboy uonessqo ISITION 5 Flow of Image Acqu UONONPOIUT SIOJ UOD UIEW Time Lapse Z Stack Z Stack Time Lapse A mo Wn U v Q Vv 2 zi jae r ab aD SE SHE e gt NE MO 4 uonis inboy uonessqo Unnecessary As required Mandatory 6 Image Editing Analysis Switching to the Review Software y Click on the ML MH button on the toolbar to switch to the Review software i os c Ha Rs ma wane Bim GITA aic awe too ege j2 The Review software provides the following functions e LUT modification e Tile image display e Maximum Intensity Projection image creation e Annotation addition e 3D image building e Time lapse image file editing using Partial Open e Stitch image creation using MATL Viewer e File export e Addition of user ID The software also incorporates various other functions For details open the Help menu and launch the Online Help uononpoul SIOJ U07 ulEW MO 4 uonis inboy uonessqo Observation Acquisition Flow Introduction Main Controls 7 System Termination gt Theoretical Infoermetian Click on the P button on the toolbar to exit the software PFFF UEF LOG w IP urg LPL ire Zoom Z Stack Stes Other Parameter Shut down Windows Vista Lapin a End 120 Set the main switch of the display to
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