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1. Pulse Amplitude Modulated PAM Fluorometer Pulse amplitude modulation is a process that uses varying light intensities delivered in a series of pulses for looking at fluorescence quenching to determine photosynthetic efficiency of algae or plant material There are two measurement modes single and multiple turnover The Ballast Check 2 is a multiple turnover PAM fluorometer in that one saturating pulse of light is delivered to the sample to quickly reach the maximum fluorescence value with the interest of only looking at how efficient algae are at photosynthesizing essentially determining the activity of the organisms High degree of activity means a high survival rate and low activity means the algae aren t capable of efficiently photosynthesizing and have a low survival rate 1 3 Quick View Diagrams The Ballast Check 2 uses four standard AAA user replaceable batteries Ballast Check 2 User s Manual 4 1 4 Inspection Upon receiving your instrument please inspect everything carefully and make sure all accessories are included The Ballast Check 2 package includes The Ballast Check 2 4 AAA batteries Solid Secondary Standard Two Glass 10x10mm Cuvettes One 60cc plastic syringe Five 50u filter capsules Five 10u filter capsules Five 0 2y filter capsules Carrying Case USB Flash Drive with User s Manual and Basic Instructions 1 5 General Information Precautions and Cleaning e Use caution around solvents they may d
2. pressed the following message will display Warning Memory gt 90 Full Indicating that you have reached more than 90 of the total memory allowed You will be allowed to continue sampling but it is highly recommended users stop analyzing samples at this point download data to their PC or computer and clear the data log When memory is full the instrument will display Memory Full When READ is pressed you will not be allowed to analyze samples until data have been downloaded and cleared from memory Ballast Check 2 User s Manual 11 3 Sample Analysis Guidelines e Take care not to spill samples into the sample chamber Wipe up any spills promptly e The cuvette MUST BE DRY on the outside when taking readings Any moisture or condensation on the outside of the cuvette can affect the reading e Fill the cuvette with at least 3 5 mL volume 3 4 full Significant error in readings may result if the cuvette contains less than this minimum volume e Use the same cuvette for your samples it is very important that you thoroughly rinse the cuvette between samples The Ballast Check 2 is very sensitive therefore cross contamination between subsequent samples will skew results Three rinses with the sample intended for measurement will help flush out any residual and decrease the chance for cross contamination e Any bubbles in the sample will affect the readings Take care not to introduce bubbles into samples Remove
3. you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at support turnerdesigns com Turner Designs will provide a WEEE RMA Number a Shipping Account Number and a Ship to Address Package and ship the product back to Turner Designs The product will be dealt with per Turner Designs end of life recycling program in an environmentally friendly way Ballast Check 2 User s Manual 3 1 Introduction 1 1 Description The Ballast Check 2 is configured to provide users with a rapid estimation of algal abundance Corrected Fluorescence or CFI and photosynthetic efficiency Activity of algae in water The CFI see section 4 1 value represents varying algal abundances used for determining gross exceedance of algae in ballast water samples around the set threshold level Activity estimates are logged as a ratio Fv Fm on a scale from 0 00 to 1 00 see section 4 2 Abundance reporting is based on a fluorescence threshold Low Corrected Fluorescence lt 100 High Corrected Fluorescence 2 100 Activity reporting is based on a yield threshold Low Activity lt 0 3 High Activity 2 0 3 1 2 PAM Fluorometry The Ballast Check 2 is a
4. 0 and 50 micron sized filters These filters are used to fractionate samples to measure different sized algae Each fractionated sample will be measured for fluorescence and the corrected fluorescence value calculated at the end of the analysis will represent the 10 50 micron sized algal cells The procedure in section 2 5 outlines how and when to use the filters provided to perform the analysis Ballast Check 2 User s Manual 8 2 5 Measuring a Sample The following materials are required for measuring a sample 60cc plastic syringe with male luer lock end 50 micron mesh filter capsule 10 micron mesh filter capsule 0 2 micron mesh filter capsule Glass Cuvette Ballast Check 2 Solid Secondary Standard Wipes for wiping the outside of the glass cuvette The following procedure is used to measure a sample Turn the Ballast Check 2 ON Set the SHIP Number and TANK Number Using the 60 cc syringe collect 60 ml of sample by aspirating from the source water Attach a 50 micron filter to the end of the plastic syringe Obtain a glass cuvette and filter some of the sample through the 50 micron filter into the glass cuvette rinse the cuvette 2 3 times with the 50 micron filtered sample before filling the glass cuvette with the filtrate 6 Wipe the outside of the glass cuvette insert the cuvette into the Ballast Check 2 and close the sample compartments lid 7 Press lt READ gt to measure the 50 micron filtered sam
5. TURNER W DESIGNS Reliable Instruments for an Unreliable World Ballast Check 2 Handheld PAM Fluorometer TURNER We DESIGNS December 5 2014 P N S 0156 Revision DRAFT TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 Table of Contents 1 Introduction 1 1 Description 1 2 PAM Fluorometry 1 3 Quick View Diagrams 1 4 Inspection 1 General Information Precautions amp Cleaning 2 Instrument Operation and Calibration 2 1 Button Identification and Function 2 2 Instrument Power Up 2 3 Calibration Verification 2 4 Filtering Samples 2 5 Measuring a Sample 2 6 Data Downloading Sample Analysis Guidelines Measurement Parameters 4 1 Corrected Fluorescence CFI 4 2 Photosynthetic Efficiency Activity 4 3 Calculations 5 Warranty 5 1 Terms 5 2 Warranty Service 5 3 Out of Warranty Service Appendixes A Specifications ankA A Ballast Check 2 User s Manual WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT WEEE DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well being of our environment Accordingly we are concerned with preserving the surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products WEEE Return Process To arrange the return of an end of life product proceed as follows If
6. any bubbles by lightly tapping with your finger on the outside cuvette wall or cover the top of the cuvette and tilt the sample to help dissipate bubbles e Wait at least 3 seconds between consecutive measurements to allow the optics to return to normal state Ballast Check 2 User s Manual 12 4 Measurement Parameters 4 1 Abundance Corrected Fluorescence CFI The Ballast Check 2 is configured for detecting fluorescence from chlorophyll in live algal cells i e in vivo detection of Chlorophyll Light from the fluorometer is absorbed by algal cells and fluorescence emitted by the cells is detected quantified and displayed as a digital number CFI estimating the abundance of algae in the sample Environmental conditions presence of interfering compounds cellular physiology and light history can influence abundance estimates The procedure used to analyze samples on Ballast Check 2 accounts for most of the interferences that may skew results and provides an accurate estimate of algal abundance for 10 50 micron sized cells 4 2 Activity Photosynthetic Efficiency The Ballast Check 2 uses two measuring LEDs to estimate photosynthetic efficiency The first LED monitoring is used to excite the sample with very low light intensity so as not to induce a change in chlorophyll reaction centers While continuously monitoring the sample using the monitoring LED the second LED saturating blasts the sample with a high intensity of l
7. computer after they ve been logged Date Time Ship Number MM DD YYYY hh mm IMO Sample Number Abundance Corrected Fluorescence Activity Photosynthetic Efficiency Sample XX CFI XXXX XX Activity X XX Tank Number XXXXXXXXXX Figure 1 2 6 Data Downloading The Ballast Check 2 can log a total of 1000 data points that can be downloaded directly to your PC or computer using the provided serial cable A terminal program e g Hyperterminal Terra Term MotoCross is required for downloading these stored ASCII data from the instrument The following settings are required for connecting the Ballast Check 2 with your terminal program Baud Rate 57600 Ballast Check 2 User s Manual 10 Stop Bits 1 Data Bits 8 Parity None Flow Control None Once connected to the Ballast Check 2 _ Set the terminal program to capture or save text Press the DATA button Use the up and down arrow keys to select SEND CLEAR DATA Press ENTER oa W PP Select SEND DATA Press ENTER Stop text capture in the terminal program Note txt files can be viewed using most all programs including Microsoft Excel and can be saved as csv or other file formats N O Confirm data are saved to your PC before proceeding to clear data from the Ballast Check 2 The Ballast Check 2 s memory starts at 100 for 1000 data points If the memory is more than 90 full and the READ button is
8. e for all customers in the contiguous continental United States For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 5 3 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs 845 W Maude Avenue Sunnyvale CA 94085 Ballast Check 2 User s Manual 14 Ballast Check 2 User s Manual 15 Appendix A Specifications Ballast Check 2 Maximum Range Sensitivity Warm Up Time Auto Power Off Light Source Detector LCD Display Error messages Resolution Case Temperature Internal Memory Data Output Format Weight Size 100 cells ml lt 10 cells ml 5 seconds After 3 minutes of inactivity LED Photodiode 2 x 16 characters Low battery Low Data Memory 12 bits Meets IP 67 Standard dustpr
9. egrade the plastic case of the Ballast Check 2 e fa sample is accidentally spilled inside the sample compartment invert the Ballast Check 2 to drain out the excess liquid Then wipe the inside area dry with a clean soft towel or tissue e f extra cleaning is needed use a mild detergent to dampen the towel for cleaning e Although the Ballast Check 2 floats do not submerge it in water e Do not expose the Ballast Check 2 to temperatures outside the specified range of 5 to 40 C or damage may occur to the unit that will not be covered under warranty Ballast Check 2 User s Manual 2 Instrument Operation and Calibration 2 1 Button Identification and Function SHIP Displays the ship number as a 10 digit alphanumeric value always beginning with the IMO prefix Only the 7 digits following the IMO prefix can be adjusted to set the ship number After setting the ship number ENTER is pressed to save the value If ESC is pressed at any time the screen is exited and nothing is saved Saved values are appended to a data point after a sample is analyzed The sample number will count sequentially beginning with Sample 1 for each set SHIP number For example if the SHIP number is changed the sample count will reset to start with Sample 1 for that newly set SHIP number TANK Displays the tank number as a configurable 10 digit alphanumeric value All characters in the alphanumeric value can be adjusted to set a desi
10. ight to effectively close chlorophyll reaction centers and bring algae to a maximum fluorescence state Fm The difference between the monitoring LED s measurement of the maximum Fm and minimum Fo fluorescence states is called variable fluorescence Fv The ratio Fv Fm is a good measure of the algal activity as it estimates the photosynthetic efficiency of algae Activity is measured on a range from 0 00 1 00 typical values for active eukaryotic algal cultures in the 10 50 micron size range can vary from 0 50 0 70 4 3 Calculations Corrected Fluorescence CFH 50 um RFU 0 2 um RFU 10 um RFU 0 2 um RFU Where 50 um RFU is the fluorescence response from the 50 um filtered sample 10 um RFU is fluorescence response from the 10 um filtered sample 0 2 um RFU is fluorescence response from the 0 2 um filtered sample The 50 and 10 um filtration steps are used to isolate the desired size class and the 0 2 um filtration is used to correct for background fluorescence Fm 50 um RFU 0 2 um RFU Fo 50 um RFU 0 2 um RFU Fm 50 um RFU 0 2 um RFU Activity Where Fm represents the maximum fluorescence value resulting from a saturation pulse Fo is fluorescence response of the algal sample using low light intensity Ballast Check 2 User s Manual 13 5 Warranty 5 1 Terms Turner Designs warrants the Ballast Check 2 PAM Fluorometer and accessories to be free from defects in materials a
11. is pressed the instrument will send all logged data out as an ASCII file see section 2 6 o If CLEAR DATA is selected the following message will display ENTER TO CONFIRM CLEAR DATA when ENTER is pressed all logged data will be deleted If SET DATE TIME is selected press ENTER and date and time will display on the screen as MM DD YYYY and hh mm Use the up down arrow keys to select either parameter to set a marker will indicate selection o If MM DD YYYY is selected press ENTER and you will be allowed to adjust the month day and year using the up down left right arrow keys Press ENTER to save the set date If ESC is pressed at any time the screen is exited and nothing is saved Date is logged per sample o If hh mmis selected press ENTER and you will be allowed to adjust the hour and minutes using the up down left right arrow keys Press ENTER to save the set time If ESC is pressed at any time the screen is exited and nothing is saved Time is logged per sample ESC Exits to the previous screen or menu CHK STD Is used to determine instrument performance PASS or FAIL will display after measuring the solid standard using the CHK STD button indicating whether the Ballast Check 2 can continue to be used for sample analysis or should be returned to the factory Ballast Check 2 User s Manual 7 2 2 Instrument Power Up Press the lt ON OFF gt button to power on the Ballast Check 2 After a 5 second warm up the Bal
12. last Check 2 is ready for operation Pressing the lt ON OFF gt button again will turn the unit off or if left idle for 3 minutes the unit will automatically turn off to save battery power HOME SCREEN The home screen will display the current date time Ballast Check 2 firmware version and remaining battery power as a percentage These parameters will be displayed after powering the instrument on and warm up has completed as well as every time ESC button is pressed to exit to the home screen 01 06 2000 11 30 FWO0 01 Batt 20 If the batteries have low power the following warning message will display Battery Low Power Replace Battery You will not be able to make any measurements until batteries are replaced 2 3 Calibration Verification The Ballast Check 2 is factory calibrated and should remain calibrated for many years Calibration can be verified using the Solid Secondary Standard SSS included with the instrument To check instrument calibration 1 Press the lt ON OFF gt button to turn the Ballast Check 2 on 2 Insert the SSS after warm up has completed and close the sample compartment lid 3 Press the CHK STD button a If the calibration is valid the Ballast Check 2 will display PASS b If the Ballast Check 2 is out of calibration and needs to be sent back to the manufacturer FAIL will display 2 4 Filtering Samples The Ballast Check 2 comes with 3 types of filters that are used to analyze a sample 0 2 1
13. nd workmanship under normal use and service for a period of 12 months from the data of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for incidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Damage incurred in shipping is not covered 5 2 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 3 If proper performance is not obtained you will be issued a Return Authorization number RMA Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charg
14. oof and waterproof 41 104 F 5 40 C 1000 records ASCII 0 87 lbs 0 4kg 1 75 x 3 5 x 7 25 4 45cm x 8 9cm x 18 4cm Ballast Check 2 User s Manual 16
15. ove the glass cuvette from the Ballast Check 2 discard the sample and rinse the glass cuvette with pure water 20 Rinse the syringe with pure water Values for Abundance and Activity are defined by the set fluorescence threshold which correlates to the IMO D2 discharge standard of less than 10 living organisms of the 10 50 micron size class in 1 milliliter of ballast water The Abundance reporting is based on the numerical part of the discharge standard 10 organisms of the 10 50 micron size class per milliliter and termed Corrected Fluorescence CFI If the CFI measured is greater than the set fluorescence threshold then HIGH and the associated fluorescence value are displayed indicating a gross exceedance of algal abundance If the CFI measured is less than the set threshold then LOW and the associated fluorescence value are displayed indicating a very low abundance of algae The Activity reporting of either LOW or HIGH is based on the activity part of the discharge standard living or active organisms and is measured as photosynthetic efficiency of the algae If the calculated Activity is greater than 0 3 then HIGH is displayed indicating a high degree of algal activity i e live algae If the calculated Activity is less than 0 3 then LOW is displayed indicating low algal activity or algae that have little activity and very low survival rate The following parameters are recorded for each single measurement and can be downloaded to a
16. ple 8 Remove the glass cuvette and discard the sample 9 Remove the 50 micron filter from the syringe and attach a 10 micron filter to the end of the 60cc syringe 10 Filter some of the remaining sample in the syringe through the 10 micron filter into the glass cuvette rinse the cuvette 2 3 times with the 10 micron filtered sample before filling the glass cuvette with the filtrate 11 Wipe the outside of the glass cuvette insert the cuvette into the Ballast Check 2 and close the sample compartments lid 12 Press lt READ gt to measure the 10 micron filtered sample 13 Remove the glass cuvette and discard the sample 14 Remove the 10 micron filter from the syringe and attach a 0 2 micron filter to the end of the 60cc syringe 15 Filter some of the remaining sample in the syringe through the 0 2 micron filter into the glass cuvette rinse the cuvette 2 3 times with the 0 2 micron filtered sample before filling the glass cuvette with the filtrate 16 Wipe the outside of the glass cuvette insert the cuvette into the Ballast Check 2 and close the sample compartments lid 17 Press lt READ gt to measure the 0 2 micron filtered sample OFT Ballast Check 2 User s Manual 9 18 After the final sample is measured the screen will display results as Abund LOW or HIGH and the associated fluorescence value as CFI Activity LOW or HIGH AII values in Figure 1 will be logged internally and can be downloaded at a later time 19 Rem
17. red TANK number After setting the TANK number ENTER is pressed to save the value If ESC is pressed at any time the screen is exited and nothing is saved Saved values are appended to a data point after a sample is analyzed The sample number will count sequentially beginning with Sample 1 for each set TANK number For example if the TANK value is changed the sample count will reset to start with Sample 1 for that new set of TANK values READ Begins the sample analysis procedure detailed in section 2 5 only if SHIP and TANK values are saved If SHIP and TANK values are not saved pressing READ will display the message First Set SHIP and TANK Values and you will not be allowed to continue with measuring a sample ON OFF Turns the instrument ON or OFF ENTER This button is used to save set values when setting SHIP or TANK values and allows users to proceed to the next screen menu Ballast Check 2 User s Manual 6 DATA When the DATA button is pressed users can choose between either sending clearing data or setting date time Use the up down arrow keys to choose between the two options a marker will indicate the selection made If SEND CLEAR DATA is selected press ENTER and a submenu will display Users can choose using up down arrow keys to either send data to a connected computer see section 2 6 or clear logged data a marker will indicate the selection made o If SEND DATA is selected and ENTER

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