Series 1400 Pulsatile Blood Pumps User`s Manual
Contents
1. Piston Diameter 1 4 in 3 4 in 1 1 8 in 2in Ball Valve Diameter 5 16 in 5 16 in 1 2 in 5 8 in Dimensions 312 x 156 x 250 mm 312 x 156 x 250 mm 500 x 212 x 337 mm 500 x 212 x 337 mm 12 5 x 6 25 x 10 in 12 5 x 6 25 x 10in 20x8 5x13 5in 20x 8 5 x 13 5 in Weight 7 3 kg 16 Ib 7 3 kg 16 Ib 13 6 kg 30 Ib 14 5 kg 32 Ib Power Used 50 W 50 W 85 W 85 W Voltage 115 230 V 115 230 V 115V 115V 50 60 Hz 50 60 Hz 50 60 Hz 50 60 Hz Application Mice Rats Rabbits Dogs Monkeys Large Animals Hemodynamic Studies Publication 5413 001 REV C s Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Operation Installing the Pump Both of the smaller pumps 1405 and 1407 are equipped with a voltage selector switch for either 120 or 220 volt usage BE SURE THIS SWITCH IS SET FOR YOUR VOLTAGE For 220 volt operation the American line cord plug must be cut off and an appropri ate plug installed The line cord is color coded in International standard colors Brown High Blue Neutral Green Ground Observe these polarities PUMPS ARE NOT EXPLOSION PROOF Operation for All Pumps The inlet nozzle is always at the bottom of the pump head Pumps are self priming and will lift liquids from reservoirs up to 50 centimeters below the inlet Since the valves of the pump are passive ball valves liquid will flow thru the pump without the pump running if 1 The inlet reservoir is above the outlet 2 The inlet pressure is higher than2. Elastomer Valves O Rings Aluminum Coupling Nut Publication 5413 001 REV C jagrams D Single Phase Motor Speed Control Models 1405 amp 1407 8l MOVIE 8i MOVIE 8l 3Mma 8 3nd dV ZH 09 05 OWA OF2 SLL dV 2 1 ZH 0S OWA ObZ dWY L ZH 09 OWA SII dV 2 1 ZH 0S OWA OpZ dW 1 ZH 09 OWA SLI dV ZH 09 05 OWA OF2 SLL 1 3MOd L04 Vecrl eel Vier levt SOvl ON I PON zSS6 25 eLee ss goge ss 6eee ss zee ss 8 81 SS ON ojeg SISVHO NIV TANVd LNOYS 9M1 GNNOYD 9M ONNOY9 81 N33Y9 HOLIMS H3MOd Is 81 MOTIJA NIJH9 8 3M8 JIYA OV 9Md E a m 7 8 ILIHM 11H97 LOMd MOVIE 81 NMOUE MOVIE isa M078 MOTIS ISNA ld 8 MOVIE JenueW sasn sdwnd poojg ajiiesind OOF Ss l1ag Ssnjeseddy puealeH Publication 5413 001 REV C 3 Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Data Hemolysis Test Data Models 1421 amp 1423 In these studies a reservoir of 500 to 800 cc of fresh dog blood was used connected to the pump by 3 8 PVC tubing Samples at room temperature were taken at 15 and 30 minute intervals for 4 to 5 hours Samples were spun down and hemolysis meas ured immediately using the method of FLINK amp WATSON Since the rate of hemoly sis depends on the amount of blood in the system and the flow rate the results are reported as mg per pass The flow rate divided by the volume of blo
3. the Standard 10 cc 1405 Pumping Head Follow the instructions below for removing the Head 1 Using the Stroke Rate and Stroke Volume Controls allow the Piston to recede to the furthest end of its travel Note The stroke volume control should be set for maximum volume delivery 2 Remove the thumbscrews attaching the cylinder and the valve head 3 Gently pull the Cylinder away from the black plate until it clears the short bind ing posts extending from this plate 4 Slide the Head and Cylinder assembly off the piston 5 If Piston removal is desired hold the Piston in one hand and use a small open end wrench to loosen the hex Coupling The Piston can then be unscrewed from the Coupling Cleaning and Sterilization For non sterile applications clean parts from all residues with water and a damp cloth Flush the head and valves before reassembly to make sure that all cloth fibers and other materials are cleared For sterile applications either gas or cold steriliza tion agents can be used Lubrication Apply a light coating of the silicone grease provided to the Piston and the inside of the Cylinder Valves The Valves are easily disassembled for cleaning The O rings can be removed for cleaning and replacement Refer to the drawing when the components are to be reassembled Pump Mechanism The entire pump mechanism is available for inspection and lubrication by removing the bottom cover and the control panel assembly Th
4. 1 ig 5124 001 Part No Publication 5413 001 REV C Replacement Part Cylinder Head Valve Nut Coupling Nozzle Suction Body Valve Nozzle Discharge Piston Shaft Connecting O Ring Parker 2 126 O Ring Parker 2 18 O Ring Parker 2 119 Set Screw 8 32 x 3 16 Sphere 1 2 D 4 Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Drawing and Parts List 100 cc 100 cc Standard Pumping Head 55 3305 1423 1403 022 Y f gt 1403 019 gt Gi SN 1 1403 020 5013 028 1403 010 1403 014 5115 011 O 5124 002 Publication 5413 001 REV C di f 1403 008 Pr 5013 029 Part No 1403 008 1403 019 1403 010 1403 020 1403 021 1403 022 1403 014 1401 015 5013 028 5013 029 5013 037 5115 011 5124 002 Replacement Part Cylinder Head Valve Nut Coupling Nozzle Suction Body Valve Nozzle Discharge Piston Shaft Connecting O Ring Parker 2 140 O Ring Parker 2 133 O Ring Parker 2 24 Set Screw 8 32 x 3 16 Sphere 3 4 D Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Diagrams Typical Pump Head Output Polycarbonate Nozzle Transparent Acrylic Valvecage Teflon Coated Piston Transparent Acrylic Chamber Silicone __ _ Transparent Acrylic Cylinder Ball Check Fluoro
5. 13 001 REV C 5013 016 1407 013 j 0680 067 Part No 0680 067 1407 013 1405 044 1405 045 1407 012 1405 046 5011 019 5013 016 5013 019 1405 043 Replacement Part Coupling Piston Body Valve Nozzle Suction Cylinder Nozzle Discharge Ball SS Bearing Hard O Ring Parker 0125 070 O Ring Parker 2 13 Head Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Drawing and Parts List 10 cc 10 cc Standard Pumping Head 55 1838 1405 1405 046 g 5013 034 1 C 1405 042 dy 5011 019 1405 045 Publication 5413 001 REV C 1405 011 1405 010 0680 067 5013 018 Part No 0680 067 1405 010 1405 044 1405 045 1405 046 1405 042 5011 019 5013 018 5013 019 Replacement Part Coupling Piston Body Valve Nozzle Suction Nozzle Discharge Assy Valve HD amp Cylinder Ball SS Bearing Hard O Ring Parker 2 16 O Ring Parker 2 13 3 Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Drawing and Parts List 30 cc 30 cc Standard Pumping Head 55 3321 1421 1401 033 AY O 5013 014 1401 032 L a Hh 1401 014 5115 011 CA gt 1401 030 1401 008 Nt H s ah 5013 032 1401 008 1401 031 1 401 030 1401 010 1401 031 1401 032 1401 033 1401 014 1401 015 5013 014 5013 021 5013 032 5115 011 5124 00
6. 1371 using our U S only toll free number 1 800 272 2775 or dial 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for com pletion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If prac tical enclose a sample or drawing We offer a complete reconditioning service AN CAUTION Not for clinical use on human patients Publication 5413 001 REV C s Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Theory of Operation This family of four pumps all similar in concept but differing in capacity are intend ed to pump blood with minimum damage to blood cells All pumps feature a mechan ically activated piston moving back and forth in a transparent cylinder The geometry of the piston actuation is such that the piston travels to the very end of the cylinder regardless of the stroke volume sel
7. Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 5 Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Table of Contents SUBJECT PAGE NO Warranty and Repair Information sss0seee 2 Theory Of Operation cccccsscsssseseeeeeeeeeeneeeeeseeseeeeeees 3 Specifications iiuair seers cenedesctececeutenaleniestee 4 Operation Installing the PUMP csssvcacccittencisiviantcaecenrermaadeuatel 5 Operation for All PUMPS eseeeeeeeeeeeeees 5 Operating Controls Stroke Volume Adjustment ssssssssissrieeeeereee 6 Rate Adjustment sax ovrvccisbinsecabeadepixtes betectvevindss 6 Output Phase Fan caer iota eastern 6 Dual Phase Rate Compensation Chart 7 Models 1405 amp 1407 Pumping Head areca dreecus i teebagencaamecetecteec fin inen 8 Cleaning and Sterilization ccccesseeseseeeeeeeeeeees 8 RSI GCAU OR seecae lag ie tea ee eae Dat tis 8 VANVOS aissent acien aa T a OAE aA 8 Pump Mechanism sssssssssssssssssrrnnerreeeerernrenn 8 Models 1421 amp 1423 PUDDING Fead serisonu ia a 9 Cleaning and Sterilization v ccccssssssssssesssssesseees 9 Lubricator riora a EEEE EE E 9 VaNVOS e Me a AA A A A lata 9 Pump Mechanism ssvsSecterelviennesDepixndbeetecdiantioe
8. Series 1400 Pulsatile Blood Pumps User s Manual Model 1405 PBP for Rabbits Model 1407 PBP for Mice Rats Model 1421 PBP for Dogs Monkeys Model 1423 PBP for Large Animals Hemodynamic Studies MAI 55 1838 MAI 52 9552 MAI 55 3321 MAI 55 3305 HARVARD APPARATUS Publication 5413 001 REV C WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either
9. e bottom cover is removed by loosening the screws within the four rubber feet The control panel assembly is removed by 1 loosening the set screw on the Stroke Volume Control and removing the knob 2 removing the eight Phillips type screws connecting the panel assembly to the housing and 3 lifting the panel assembly clear of the stroke control shaft IMPORTANT Make sure that the wires remain connected to their appropriate ter minals All bearings and points of frictional contact should be lubricated every 30 days with a light machine oil Harvard Part No 0606 060 Publication 5413 001 REV C s Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Operation Models 1421 amp 1423 Care and Maintenance Disconnect from the power source before performing any motor maintenance Pumping Head The Pump Head is easily removed for cleaning sterilization and lubrication Refer to diagrams for part designation on the 1421 and 1423 Pumping Heads respectively Follow the instructions below for removing the head 1 Using the spanner wrench provided insert the wrench pin into one of the holes in the Nut Coupling apply pressure and loosen The coupling can now be eas ily removed from the cylinder 2 Using the same wrench insert the wrench pin into one of the holes on the cylin der apply pressure and loosen Proceed to unscrew the cylinder manually 3 To remove the Piston insert the wrench pin into one of the hole
10. ected This feature insures that blood is completely emptied from the cylinder at each stroke The valve cages and liquid pathway have been carefully designed to provide a stream lined flow with no sharp edge to minimize hemolysis All parts of the liquid pathway can be disassembled for cleaning and sterilization In all pumps the volume per stroke and the strokes per minute can be adjusted while the pumps are running The two smaller pumps 1405 and 1407 have mechanically fixed systole diastole ratios of 33 that is diastole lasts twice as long as systole The larger pumps 1421 and 1423 have electronically variable ratios in which systole can be adjusted to be 25 to 50 of the total pumping cycle Publication 5413 001 REV C Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Specifications The specifications of all pumps are compared in the following chart Harvard Pulsatile Blood Pump Comparison Chart Catalog No 52 9552 55 1838 55 3321 55 3305 Model No 1407 1405 1421 1423 Stroke Volume 0 05 to 1 0 ml 0 5 to 10 0 ml 4 to 30 ml 15 to 100 ml Stroke Rate per min 20 to 200 20 to 200 20 to 200 10 to 100 Minimum Volume vol x rate 1 to 200 ml 5 ml to 2 0 liters 80 ml to 6 liters 150 ml to 10 liters Phasing Fixed Phase Fixed Phase Adjustable Phase Adjustable Phase 35 systole 35 systole 35 to 50 35 to 50 65 diastole 65 diastole of total cycle of total cycle Tube Size ID 5 16 in 5 16 in 1 2 in 5 8 in
11. od in the sys tem determines the number of passes through the pump per minute Hemolysis ranged from 0 114 mg to 0 27 mg per hour pass through the various pumps with an error of 10 To put these results in perspective most physiological perfusions are run with flow rates and total blood primes so that the number of passes through the pump will range from about 1 4 to 3 4 per minute Assuming one pass in two minutes and no physiological removal of the products of hemolysis then hemolysis rates would range from 3 4 mg to 8 7 mg per hour of pump use Publication 5413 001 REV C
12. s 9 CONTON ie 25 25 a E a E a A EE 9 Drawing and Parts List cccecceeeeeeeseeeeeeees 10 13 Di graMS sis cai ccatencacenn aaaea aaraa iaip arapen anaiai 14 15 Data iiin a aar a aa ieia aaaea 16 Publication 5413 001 REV C Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Warranty and Repair Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit s Warranty Harvard Apparatus warranties the instrument s for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit s if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts_01746
13. s on the Piston apply pressure and loosen Remove the Piston manually Cleaning and Sterilization For non sterile applications clean parts from all residues with water and a damp cloth Make certain that all cloth fibers are cleared from the system before it is reassembled For sterile applications either gas or cold sterilization agents can be used Lubrication Apply a light coating of the silicone grease provided to the Piston and the inside of the Cylinder Valves The Valves are easily disassembled for cleaning The O rings can be removed for cleaning and replacement Refer to the drawing when the components are to be reassembled Pump Mechanism The entire Pump Mechanism is available for inspection and lubrication by removing the blank side panel All bearings and points of frictional contact should be lubricat ed every 30 days with a light machine oil Harvard Part No 0606 060 Control The solid state dual phase Motor Control needs no special attention If a problem seems to exist in the motor control the unit should only be examined by a qualified technician A safety fuse 1 amp slo blo type is provided for overload protection DO NOT REPLACE with a higher rated fuse Publication 5413 001 REV C Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual 5 Drawing and Parts List 1 cc 1 cc Standard Pumping Head 52 9552 1407 1405 046 g 5013 034 l 1 O 1405 043 Publication 54
14. th a control to adjust the ratio of systole to dias tole This control is located adjacent to the rate control The control is continuously variable from 25 75 to 50 50 In the 25 75 position systole takes place in 25 of the cardiac cycle while diastole takes place in 75 This is observed by watching the pis ton cylinder eject liquid quickly and fill slowly in the 25 75 position In the 50 50 position the piston speed is the same in filling and emptying The phase ratio has an affect on the rate control see figure 1 on next page The observed stroke rate varies considerably from the set rate as the phase ratio reaches its outer limits Publication 5413 001 REV C Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Operation 50 50 50 45 55 40 60 35 65 30 70 40 25 75 lt E a 30 N g a D amp 20 T lt 10 0 0 10 20 30 40 50 Rate on strokes per min dial Figure 1 Dual Phase Rate Compensation Chart Phase ratio systole typical plot of actual stroke rate at various ratios and strokes Publication 5413 001 REV C 3 Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Operation Models 1405 amp 1407 Care and Maintenance Disconnect from the power source before performing any motor maintenance Pumping Head The Pumping Head is easily removed for cleaning sterilization and lubrication Refer to the diagram for part designation on
15. the outlet pressure 3 The outlet tubing is below the inlet this can cause siphoning or gravity induced flow The volume pumped per minute is called the minute volume It is the product of the stroke volume times the stroke rate This is only valid if there is a normal amount of back pressure or resistance to flow Since the pumped liquid has inertia liquid will continue to flow even in the filling cycle This can be prevented by having adequate resistance in the output circuit Publication 5413 001 REV C 4 Harvard Apparatus Series 1400 Pulsatile Blood Pumps User s Manual Operation Operating Controls Stroke Volume Adjustment On smaller pumps the volume is set by turning the stroke volume control located at the top of the pump Volume is read from the decal fastened to the pump cylinder Volume is determined by watching the excursion of one O ring Volume should be adjusted while the pump is running A thumb screw is built in to the volume control to provide positive locking once the volume is set On larger pumps the volume control is at the top while the actual volume is read from a calibrated plate on the side of the pump A locking knob next to the volume pointer provides positive locking Rate Adjustment strokes per min On small pumps the rate control is at the top of the pump on large pumps the rate control is at the end of the pump opposite the pump head Output Phase Ratio The larger pumps are equipped wi
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