Akta prime
Contents
1. Chemical Exposure Exposure Comments lt 1 day upto2 months Glycerol 100 OK OK 56 81 5 200 289 5 Guanidinium hy OK OK drochloride Hexane OK Avoid Silicone not resis tant Pressure limit for PEEK decreases Hydrochloric acid OK OK 7647 01 0 231 595 7 Silicone not resis 0 1M tant Hydrochloric acid OK Avoid Silicone not resis gt 0 1M tant Titanium is af fected by long term use Isopropanol 100 OK OK 67 63 0 200 661 7 Methanol 100 OK OK 74 93 1 200 659 6 Nitric acid diluted OK Avoid Silicone not resis tant Nitric acid 30 Avoid Avoid Elgiloy is affected by long term use Phosphoric acid OK Avoid 7664 38 2 231 633 2 Titanium alumini 10 um oxide and glass are affected by long term use Potassium carbon OK OK 584 08 7 209 529 3 ate Potassium chloride OK OK 7447 40 7 231 211 8 Pyridine Avoid Avoid ETFE PP and PE not resistant Sodium acetate OK OK Sodium bicarbon OK OK ate Sodium bisulphate OK OK 54 AkTAprime plus Operating Instructions 28 9597 89 AA 7 Reference information 7 2 Chemical resistance Chemical Exposure Exposure Comments lt 1 day up to 2 months Sodium borate OK OK Sodium carbonate OK OK Sodium chloride OK OK 7647 14 5 231 598 3 Sodium hydroxide OK Avoid 1310 73 2 215 185 5 PVDF and borosili 2M cate glass are af fected by long term use Sodium sulp2. 24 is i a a E D A E T T TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT MIN 00 0 1 02 03 04 05 06 07 08 09 1 0 11 12 13 14 15 16 17 18 19 20 Logbook aj For Help press F1 IO End No watch Controlled By prime The Curves pane displays monitor signal values graphically e The Logbook pane displays all actions e g method start and end base instructions and method instructions and unexpected conditions e g warnings and alarms The log is saved in the result file Selecting curves to be displayed 1 In PrimeView module select View Properties 2 Inthe Properties dialog click the Curves tab 3 In the Display curves list select the curves you want to display 4 Click OK For more information on customizing the view panes see PrimeView User Manual AKTAprime plus Operating Instructions 28 9597 89 AA 35 4 Operation 4 4 Performing a run Ending the run Press OK at the Method Complete prompt This will cause all valves to return to their default positions To stop the run on a system before it is finished 1 Press the end button 2 Select yes and press OK Error indication When a warning or an alarm is issued from a system an error code is displayed See AKTAprime plus User Manual for guidance Evaluate the results PrimeView Evaluation module provides facilities for the presentation and evaluation of separation results To start PrimeView Evaluation module click Pr
3. Change to smaller mixer volume AKTAprime plus Operating Instructions 28 9597 89 AA 47 6 Troubleshooting 6 2 Conductivity curve problems Error symptom Incorrect or unstable reading Possible cause Loose connection of conductivity flow ca ble Corrective action Check that the conductivity flow cell cable is connected properly to the rear of the instrument Incorrect pump and valves function Check that the pump and valves operate correctly Incorrect temperature compensation factor If temperature compensation is being used check that the temper ature sensoris calibrated and that the correct temperature compen sation factor is in use Dirty or incorrectly calibrated column Check that the columnis equilibrat ed If necessary clean the column Incorrect mixer func tion Check the operation of the mixer The mixer function is checked by placing a stirrer bar on top of the mixer housing The stirrer bar should rotate when the system is in Run mode The mixer function can also be checked by running the installation test 6 3 pH curve problems 48 Error symptom No response to pH changes Possible cause Faulty electrode con nection Corrective action Check that the electrode cable is connected properly to the rear of the instrument Damaged electrode The electrode glass membrane may be cracked Replace the elec trode Incorrectly
4. Identity of person authorized to sign DoC Peter L wendahl CE Conformity This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the Declaration of Conformity is available on request 2006 42 EC Machinery Directive MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive International standards This product fulfills the requirements of the following standards Standard Description EN 61010 1 IEC Safety requirements for electrical 61010 1 UL61010 1 equipment for measurement control CAN CSA C22 2 and laboratory use No 61010 1 8 AkTAprime plus Operating Instructions 28 9597 89 AA Standard EN 61326 1 1 Introduction 1 2 Regulatory information Description EMC emissions and immunity require ments for electrical equipment for measurement control and laboratory use Notes Harmonized with 2004 108 EC EN ISO 12100 1 12100 2 Safety of machinery Basic concepts general principles for design Harmonized with 2006 42 EC EN ISO 14121 1 14121 2 Safety of machinery Principles of risk assessment Harmonized with 2006 42 EC CE Marking The CE marking and the corresponding Declaration of conformity is valid for the instrument when it is e used as a stand alone unit or connected to other CE marked inst
5. and optional pH monitor 6 F W From the monitors the flow is directed to the Fraction collector F or the Waste W AkTAprime plus Operating Instructions 28 9597 89 AA 1 Introduction 1 4 Monitoring and evaluation 1 4 Monitoring and evaluation PrimeView software PrimeView is a software that allows real time monitoring evaluation and report generation on an external computer For more information about PrimeView evaluation system see the PrimeView User Manual supplied Paper chart recorder It is possible to connect a chart recorder to AKTAprime plus to get real time monitoring For more information see the AKTAprime plus User Manual AKTAprime plus Operating Instructions 28 9597 89 AA 13 2 Safety instructions 2 Safety instructions This chapter describes safety compliance safety labels general safety precautions emergency procedures power failure and recycling of AKTAprime plus system 2 1 Safety precautions Introduction The KTAprime plus instrument is powered by mains voltage and handles pressurized liquids that may be hazardous Before installing operating or maintaining the system you must be aware of the hazards described in this manual Follow the instructions provided to avoid personal injury or damage to the equipment The safety precautions in this section are grouped into the following categories General precautions e Using flammable liquids e Personal protection Installing and m
6. AKTAprime plus Operating Instructions 28 9597 89 AA 5 1 Introduction 1 1 Important user information 1 1 Important user information Read this before using the AKTAprime plus system ci All users must read the safety instructions in the AKTAprime plus User Documentation to fully understand the safe use of the AKTAprime plus system before installing using or maintaining the system Do not operate the AKTAprime plus system in any other way than described in the user documentation Otherwise you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Intended use AKTAprime plus is a compact liquid chromatography system designed for one step purification of proteins at laboratory scale The AKTAprime plus system is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of the product See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood 6 AkTAprime plus Operating Instructions 28 9597 89 AA 1 Introduction 1 1 Important user information Cautions CAUTION CAUTION indicates a hazardous situation which if not avo
7. Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9597 89 AA 10 2009
8. are not obtained until after 1 hour 4 3 Preparations before start Buffer preparation Prepare buffers according to AKTAprime plus cue cards Sample preparation 1 Adjust the sample composition to the binding buffer by e diluting the sample in binding buffer or buffer exchange using HiTrap Desalting or HiPrep 26 10 Desalting column 2 Filter the sample through a 0 45 um filter Purification setup Removing storage solution from the flow path At delivery and during storage the flow path is filled with 20 ethanol This should be removed before continuing the setup Note Do not use buffer with high salt concentration to flush out the ethanol It might cause too high backpressure To flush out the ethanol using deionized water 1 Put the inlet tubing A1 A8 that is used and B in deionized water Note At delivery only A1 and B are installed 2 Put all waste capillaries W1 W3 in waste Select Templates in the main menu using the A and V buttons and press OK 4 Select Application Template and press OK 5 Select System Wash Method and press OK 6 Choose to wash the A2 A8 inlet tubing that is used by pressing OK at those cursor positions A1 and B will always be washed Note At delivery only A1 and B are installed 7 Scroll to OK and press the OK button AKTAprime plus Operating Instructions 28 9597 89 AA 29 4 Operation 4 3 Preparations before start 30 8 Press OK to start the method 9 When th
9. available from your GE Healthcare representative 24 AkTAprime plus Operating Instructions 28 9597 89 AA 2 Safety instructions 2 4 Recycling information Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment AKTAprime plus Operating Instructions 28 9597 89 AA 25 3 Installation 3 Installation AKTAprime plus is delivered in protective packing material and shall be unpacked with great care Any equipment connected to AKTAprime plus must fulfill applicable standards and local regulations A video describing the installation process is supplied with each AKTAprime plus system For detailed information on Installation see AKTAprime plus Installation Guide 3 1 Site requirements Parameter Requirement Electrical power 100 240 V 50 60 Hz Ambient temperature 4 C to 40 C Placement Stable laboratory bench e g 120 x 80 cm Humidity 20 to 95 non condensing 3 2 Transport The equipment can be transported on a trolley capable of supporting at least 20 kg NOTICE Lift the system in the upright position Do not use the fractionation arm as a lifting handle Before moving the system disconnect all cables and tubing connected to peripheral co
10. the rear of the system Navigation menu The system is operated from the push buttons and LCD display at the front panel feed pause tube con Figure 1 2 Push buttons Button Description Find a specific menu option Aa V i i OK Enter a menu Esc Return one menu level end Interrupt method operation before the run is completed Stop manual operation hold cont Hold method time or volume and the gradient at the cur rent concentration Pump and fraction collector continue uninterrupted Continue the normal method operation pause cont Pause all operation without ending the method All func tions including pump and fraction collector are stopped Continue the normal method operation feed tube Advance the fraction collector one position KTAprime plus Operating Instructions 28 9597 89 AA 11 1 Introduction 1 3 Instrument Basic flow path 12 L Figure 1 5 Basic flow path Stage Part Description 1 P V1 Pump P pumps buffer from a buffer container connected to the buffer valve V1 2 SW B To form a gradient the switch valve SW can be used to pull liquid from buffer container B 3 M The mixer M mixes the buffers 4 V2 Sample is applied from the sample loop connected to injection valve V2 that has been previously filled manually using a syringe 5 UV C pH From the injection valve the flow is directed to the column and then to the UV Conductivity
11. AKTAprime plus Operating Instructions 28 9597 89 AA 33 4 Operation 4 3 Preparations before start Connect a sample loop between port 2 and 6 on the injection valve Make sure that the sample loop is large enough for your sample Connect a luer female 1 16 male union to port 3 Fill a syringe with five loop volumes of deionized water or binding buffer Fit the syringe in the Luer union 1 and carefully inject the buffer Remove the syringe and fill it with at least two loop volumes of the sample Carefully inject the sample into the sample loop Do NOT remove the syringe after the injection because the loop might otherwise be emptied due to self drainage or air may be introduced in the flow path 4 4 Performing arun Selecting template and starting the run 34 Select Templates in the main menu and press OK Select Application Template and press OK Select the appropriate template for example His Tag Purification HisTrap and press OK Set the sample volume and press OK Press OK to start the purification run AkTAprime plus Operating Instructions 28 9597 89 AA 4 Operation 4 4 Performing a run Viewing the run When the pump starts running the progress of the run can be viewed in the two panes in PrimeView 4 Prime iew prime Result C prime Manual Runs prime Manual Run 7 RES Ele view Help UV Cond jH Pressure Temp Conc 40 3 8 3 6 34 32 3 0 2 8 26
12. AKTAprime plus Operating Instructions 28 9597 89 AA 37 5 Maintenance 5 Maintenance 5 1 General Regular maintenance is important for safe and trouble free operation of your instrument The user should perform daily and monthly maintenance Preventive maintenance should be performed on a yearly basis by qualified service personnel 38 This chapter provides instructions for routine component maintenance and a maintenance schedule For maintenance of a specific component carefully read the component manual and follow the instructions To avoid personal injury when performing maintenance on the AKTAprime plus instrument follow the instructions below WARNING Electrical shock hazard All repairs should be done by service personnel authorized by GE Healthcare Do not open any covers or replace parts unless specifically stated in the user documentation WARNING Disconnect power Always disconnect power from the instrument before replacing any component on the instrument unless stated otherwise in the user documentation WARNING Hazardous chemicals during maintenance When using hazardous chemicals for system or column cleaning wash the system or columns with a neutral solution in the last phase or step AkTAprime plus Operating Instructions 28 9597 89 AA 5 Maintenance 5 1 General WARNING Do not perform any type of maintenance work while the system is powered electrically or when the
13. GE Healthcare AKTAprime plus Operating Instructions Original instructions KTA Table of Contents Table of Contents 1 oc doc Orendi ela ooo eerie nom iei bI EM LI E 11 Important user information 1 2 Regulatory information 1 5 Instrument sssss a 1 Monitoring and SVU OM s eerte eae teet i Safety MISTI DOE senc mienne 2 1 Safety precautions 22 Labels ces 2 3 Emergency procedutes sui nn ut e better oor c cH 24 Recycling InformatioE sessinum Installat MERC ests SJ Site TequiFelmiel s sse teo itte erm i ied euin dr 3 2 TIEGIPIS 0101A ER ee rte dant tte ie 32 UAC KUNG EE eerte ettet ot ede tee Du in nr a ra 3 4 Connections ss 3 5 Spare parts and accessories STIG IN SR iut enc te MEI 4 1 Operation OVerview nee 42 Starting the iristrurmerlt use nn nn boe rese uh 4 5 Preparations before start eceecccssssssssessessessccsssssssssssssssssscsssssssssusssessessecssssssssussussssssessesssssssssseseeses 4 4 Performing a run T 45 Procedures after TUN uscite tereti rere Raetia leeren inier E cu EMACCIIM T E E E a EE EEEE 5 2 User maintenance schedule th Mee m u u 5 4 Component maintenance inner 5 5 Disassembly and assembly of components and consumables s De CallBratiomisssi esee oet e ce n undc etn 5 7 St rde m M M Troubleshoot i
14. Oo D N WD OH FF ON N be Put inlet tubing A1 in deionized water Run the pump at 40 ml min for 1 min and press pause cont Move inlet tubing A1 to 100 ethanol Press pause cont run the pump for 10 20 s and press pause cont Set the flow rate to 5 ml min using the arrow buttons Press pause cont run the pump for at least 30 s and press pause cont Move inlet tubing A1 to deionized water Press pause cont and run the pump for 1 min Finish by pressing end and then OK Preparing the tubing and column 1 2 3 Put inlet tubing A1 in the binding buffer Put inlet tubing B in the elution buffer Put the three waste capillaries brown color from port 4 and 5 on the injection valve and port NO on the fraction collector valve in waste Connect a column for example a HisTrap HP 1 ml column between port 1 on the injection valve and the upper port of the UV flow cell Use a suitable length of PEEK tubing and 1 16 male connectors No Description No Description 1 From injection valve 3 HisTrap column 2 1 16 male connector 4 UV cell Note Other unions and connectors might be required for other columns AKTAprime plus Operating Instructions 28 9597 89 AA 1 4 Operation 4 3 Preparations before start Preparing the fraction collector 1 Fill the fraction collector rack with for example 18 mm tubes minimum 40 pcs 2 Adjust the height of the delivery arm using the lock knob 1 so that the bottom o
15. The section also describes the result in the event of power failure Emergency shutdown In an emergency situation do as follows to stop the run Step Action 1 To pause the run without ending the method press the Pause button located at the instrument front 2 If required switch off power to the instrument by pressing the Main power Switch to the 0 position The run is interrupted immediately AKTAprime plus Operating Instructions 28 9597 89 AA 23 2 Safety instructions 2 3 Emergency procedures Power failure The result of a power failure depends on which unit that is affected Power failure to will result in AkTAprime plus system The run is interrupted immediately in an undefined state e The data collected up to the time of the power failure is available in PrimeView Computer e The PrimeView computer shuts down in an undefined state e The run continues but data cannot be saved in PrimeView 2 4 Recycling information The equipment shall be decontaminated before decommissioning and all local regulations shall be followed with regard to scrapping of the equipment Disposal general instructions When taking AKTAprime plus system out of service the different materials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substances AKTAprime plus instrument contains hazardous substances Detailed information is
16. biological agents during run When using hazardous biological agents run System CIP and Column CIP to flush the entire system tubing with bacteriostatic solution e g NaOH followed by a neutral buffer and finally distilled water before service and maintenance 18 KTAprime plus Operating Instructions 28 9597 89 AA 2 Safety instructions 2 1 Safety precautions WARNING There must always be a sample loop connected to ports 2 and 6 of the injection valve This is to prevent liquid spraying out of the ports when switching the valve This is especially dangerous if hazardous chemicals are used for example NaOH and distilled water before service and maintenance NOTICE If the AKTAprime plus is kept in a cold room cold cabinet or similar keep the system switched on in order to minimize the risk of condensation The UV lamp can be turned off to save lamp life time when the system is not in use NOTICE When switching off the cold cabinet make sure that you also switch off the AKTAprime plus system and leave the door to the cold cabinet open to avoid overheating CAUTION Hazardous chemicals in UV flow cell Make sure that the entire flow cell has been flushed thoroughly with bacteriostatic solution Maintenance WARNING Electrical shock hazard All repairs should be done by service personnel authorized by GE Healthcare Do not open any covers or replace parts unless specif
17. connected pH monitor Check that the pH monitor is cor rectly connected according to the User Manual Small response to pH changes Dirty pH electrode Clean the pH electrode as detailed in Monitor pH C 900 User Manual If the problem remains replace the pH electrode AkTAprime plus Operating Instructions 28 9597 89 AA Error symptom Slow pH response or Calibration impossible Possible cause Contaminated elec trode glass mem brane 6 Troubleshooting 6 3 pH curve problems Corrective action Check the electrode glass mem brane If it is contaminated clean the electrode following the instruc tions in Monitor pH C 900 User Manual Membrane has dried out Ifthe membrane has dried out the electrode may be restored by soaking it in buffer overnight AKTAprime plus Operating Instructions 28 9597 89 AA 49 6 Troubleshooting 6 3 pH curve problems 50 Error symptom Incorrect or unstable pH reading Possible cause Corrective action Problem with elec Check that the electrode cable is trode connected properly to the rear of the instrument Check that the electrode is correct ly inserted in the flow cell and if necessary hand tighten the nut Check that the pH electrode is not broken Calibrate the pH electrode Clean the pH electrode if required see Monitor pH C 900 User Manual Compare the response of the pH electrode with that of anothe
18. e method is finished replace the first collection tube It will contain a small amount of water after the system wash Purging the pump and inlet tubing If there are large amounts of air in the tubing or if you suspect air in the pump use the Purge kit to purge the flow path Air bubbles that still are trapped in the pump causing increased pulsation can be removed by flushing 100 ethanol through the pump These two procedures are described in the following two sections Purging the flow path using the Purge kit 1 Remove the stop plug from the pump 2 Connect the Purge kit to the pump Pro axtaprime plus 3 Put the used inlet tubing in the appropriate buffers 4 Run the pump at 0 1 ml min Filling inlet tubing A1 A8 1 Go to Set Buffer Valve using the arrow buttons 2 Set the chosen A inlet and press OK The valve switches to the selected port 3 Draw buffer with the purge syringe until liquid enters the syringe 4 Repeatstep 1 3 until all chosen A inlet tubing is filled Filling inlet tubing B 1 Go to Set Concentration B and set the concentration to 100 2 Press OK The switch valve turns to the inlet B port 3 Draw buffer with the purge syringe until liquid enters the syringe 4 Replace the purge tubing with the stop plug 5 Stop the pump by pressing end and then OK AkTAprime plus Operating Instructions 28 9597 89 AA 4 Operation 4 3 Preparations before start Flushing the pump with 100 ethanol
19. f the tube sensor 2 is about 5 mm below the top of the tubes The tubes should always be below the horizontal mark on the tube sensor 3 If necessary adjust the length of the tubing exposed according to the sequence shown below the hole in the delivery arm used in step 3 and 4 is only used for adjusting the tubing length 32 AkTAprime plus Operating Instructions 28 9597 89 AA 4 Operation 4 3 Preparations before start 4 Check that the tube sensor 1 is in the correct position for the tube size The eluent tubing should be over the center of the collection tube Use the red sensor control knob 2 to position the tube holder 3 tube 6 Press feed tube on the front panel see Figure 1 2 The bowl moves to the correct position to collect the first fraction in the first tube 7 Make sure that drop synchronization is turned on Note Drop synchronization can NOT be used at flowrates above 3 ml min Preparing the monitors 1 Check the UV lamp filter position and the lamp position 2 Calibrate the pH electrode optional See AkTAprime plus User Manual for more information Filling the buffer inlet tubing When running an application templates the buffer inlet tubing will automatically be filled with buffer For other applications fill the inlet tubing manually with buffer as described in the AKTAprime plus User Manual Filling the sample loop Using an injection fill port
20. g L Hub pud Less CROP o9 Od G9 f 9 4 SSSSSGSSSSSSS 10 11 No Connection No Connection 1 RS 232 to computer 7 pH electrode 2 Flow diversion valve 8 Conductivity flow cell 3 Fraction collector 9 Optical unit 4 Measurement data to recorder 10 Power switch 5 On off signals to recorder 11 Power inlet 6 UV lamp Figure A 1 Electrical and communication connections for KTAprime plus KTAprime plus Operating Instructions 28 9597 89 AA 57 B Connection diagram Liquid flow path Appendix B Connection diagram Liquid flow path No Description No Description 1 Buffers 8 Loop 500 ul 2 Buffer valve 9 Waste 3 Gradient switch valve 10 Column 4 System pump 11 Male Male 5 Pressure monitor 12 Flow restrictor 0 2 MPa 6 Stop plug 13 Flow diversion valve 7 Mixer 14 Fraction collector 58 AkTAprime plus Operating Instructions 28 9597 89 AA C Tubing Appendix C Tubing Names in the Label column in Table C 1 refer to tubing labels in the liquid flow path connection diagram see Appendix B Connection diagram Liquid flow path on page 58 Table C 1 Tubing specifications for AKTAprime plus Material Length D Volume mm mm ul Inlet A11 Al Teflon 1250 29 8 2 x 10 Inlet A1 A Teflon 170 29 1 1 x 103 Inlet A2 B Teflon 1250 29 8 2 x 10 Switch valve Pump A3 AB Teflon 510 1 6 1 0 x 10 Pump Pressu
21. hate OK OK 7757 82 6 231 820 9 Sulphuric acid dilut OK Avoid PEEK and titanium ed are affected by long term use Sulphuric acid Avoid Avoid medium concentra tion Tetrachloroethy Avoid Avoid Silicone PP and PE lene are not resistant Tetrahydrofuran Avoid Avoid ETFE CTFE PP and PE are not resistant Toluene OK Avoid Pressure limit for PEEK decreases Trichloroacetic OK OK 76 03 9 200 927 2 acid 1 Trifluoroacetic acid OK OK 176 05 1 200 929 3 1 Urea 8M OK OK 57 13 6 200 315 5 o Xylene and p Xy OK Avoid PP and PE are af lene fected by long term use 7 5 System recommendations Refer to AKTAprime plus User Manual or contact your local GE Healthcare representative for the most current information AKTAprime plus Operating Instructions 28 9597 89 AA 55 7 Reference information 7 4 Literature 7 4 Literature For further information regarding the AKTAprime plus system refer to the following e AKTAprime plus User Manual e AkTAprime plus Installation Guide e PrimeView User Manual 7 5 Ordering information For ordering information visit www gelifesciences com AKTA 56 AkTAprime plus Operating Instructions 28 9597 89 AA Electrical and communication connections Appendix Electrical and communication connections di 7 2 8 A 3 eE I
22. ically stated in the user documentation AKTAprime plus Operating Instructions 28 9597 89 AA 19 2 Safety instructions 2 1 Safety precautions WARNING Disconnect power Always disconnect power from the instrument before replacing any component on the instrument unless stated otherwise in the user documentation WARNING Hazardous chemicals during maintenance When using hazardous chemicals for system or column cleaning wash the system or columns with a neutral solution in the last phase or step WARNING Do not perform any type of maintenance work while the system is powered electrically or when the piping system is pressurized Note that the piping system can be pressurized even when the system is closed down WARNING Only spare parts that are approved or supplied by GE Healthcare may be used for maintaining or servicing the system WARNING Make sure that the piping system is completely leakage free before performing any CIP on the system WARNING NaOH is corrosive and therefore dangerous to health When using hazardous chemicals avoid spillage and wear protective glasses and other suitable personal protective equipment 20 AkTAprime plus Operating Instructions 28 9597 89 AA 2 Safety instructions 2 1 Safety precautions WARNING After assembly the piping system must be tested for leakage at maximum pressure for continued protection against injury ris
23. ided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A Note is used to indicate information that is important for trouble free and optimal use of the product TIP A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 1 2 Regulatory information This section describes the directives and standards that are fulfilled by the AKTAprime plus system AKTAprime plus Operating Instructions 28 9597 89 AA 7 1 Introduction 1 2 Regulatory information Manufacturing information The Declaration of Conformity includes the following information Requirement Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Swe den Name and address of person responsible Peter L wendahl Bj rkgatan 30 for Technical File SE 751 84 Uppsala Sweden Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden May 2009
24. imeView Evaluation icon on the Windows desktop 5 PrimeView Evaluation See KTAprime plus User Manual and PrimeView User Manual for how to evaluate the results 4 5 Procedures after a run Cleaning after a run NOTICE Do not allow solutions which contain dissolved salts proteins or other solid solutes to dry out in the UV flow cell 36 AkTAprime plus Operating Instructions 28 9597 89 AA 4 Operation 4 5 Procedures after a run NOTICE Do not allow particles to enter the UV flow cell as damage to the flow cell might occur Buffers not containing any salt can be left in the system for a short time after a run even overnight not in the pH electrode see instructions below NOTICE If a buffer containing salt has been used the flow path must be flushed with deionized water To flush the flow path 1 Filla syringe with five times the sample loop volume of deionized water 2 Rinsethe sample loop by injecting the water through the fill port on the injection valve 3 Put all used inlet tubings in water 4 Inthe Templates menu select Application Template and then System Wash Method 5 Select the used inlet ports Inlets A1 and B will always be washed 6 Press OK to start the method The system flow path is now automatically flushed For information on cleaning and long term storage see Section 5 3 Cleaning on page 41 and Section 5 7 Storage on page 44
25. ks due to fluid jets burst pipes or explosive atmosphere WARNING Before disassembly check that there is no pressure in the piping system WARNING Disconnect power Always disconnect power from the instrument before replacing fuses WARNING Decontaminate the equipment before decommissioning to ensure that hazardous residues are removed CAUTION The system uses high intensity ultra violet light Do not remove the UV lamp while the system is running Before replacing a UV lamp ensure that the lamp cable is disconnected from the rear of the system to prevent injury to eyes gt PPP gt If the mercury lamp is broken make sure that all mercury is removed and disposed according to national and local environmental regulations NOTICE Cleaning Keep the instrument dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cleaning agent Let the instrument dry completely before use AKTAprime plus Operating Instructions 28 9597 89 AA 21 2 Safety instructions 2 2 Labels 2 2 Labels This section describes the safety labels and labels concerning hazardous substances that are attached to the AKTAprime plus instrument For information about marking of the computer equipment refer to the manufacturer s instructions Labels on the instrument The illustration below shows an example of the identification label that is attached to the AKTAprime plus ins
26. l noisy with water Air in the pump or in the UV cell Purge the pump according to Pump User Manual Run a system wash with buffer AKTAprime plus Operating Instructions 28 9597 89 AA 45 6 Troubleshooting 6 1 UV curve problems Error symptom Low sensitivity Possible cause Aging UV lamp Corrective action Check the lamp run time according to and replace if necessary Refer to AKTAprime plus User Manual UV lamp in wrong po sition Check that the lamp position and the filter position are both set to the wavelength to be used 280 nm or 254 nm Refer to AKTAprime plus User Manual The theoretical extinc tion coefficient too low Calculate the theoretical extinction coefficient of the protein If it is ze ro or very low at 280 nm the pro tein cannot be detected 6 2 Conductivity curve problems 46 Error symptom Baseline drift or noisy signal Possible cause Air in the pump orthe flow cell Corrective action Use a flow restrictor after the flow cell Leaking tube connec tions Tighten the clamps If necessary replace the clamps Incorrect mixer func tion Check the mixer function by plac ing a stirrer bar on top of the mixer housing The stirrer bar should ro tate when the system is in Run mode The mixer function can also be checked by running the installa tion test Dirty conductivity cell Clean the conductivity cell by f
27. lus Operating Instructions 28 9597 89 AA 15 2 Safety instructions 2 1 Safety precautions NOTICE Avoid condensation by letting the unit equilibrate to ambient temperature Using flammable liquids When using flammable liquids with the AKTAprime plus instrument follow these precautions to avoid any risk of fire or explosion WARNING Fire Hazard Before starting the system make sure that there is no leakage WARNING A fume hood or similar ventilation system shall be installed when flammable or noxious substances are used Personal protection WARNING Always use appropriate personal protective equipment during operation and maintenance of AKTAprime plus system WARNING Hazardous substances When using hazardous chemical and biological agents take all suitable protective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation and maintenance of the system 16 AkTAprime plus Operating Instructions 28 9597 89 AA 2 Safety instructions 2 1 Safety precautions WARNING Spread of biological agents The operator has to take all necessary actions to avoid spreading hazardous biological agents in the vicinity of the instrument The facility should comply with the national code of practice for biosafety WARNING High pressure The AKTAprime plus inst
28. lushing 1 M NaOH or 20 ethanol Column not equilibrat ed Equilibrate the column If neces sary clean the column using a method plan for column cleaning Conductivity measure ment with the same buffer appears to de crease over time Dirty flow cell Clean the flow cell according to procedure in Monitor User Manual Decrease in ambient temperature Use a temperature compensation factor See Monitor User Manual AkTAprime plus Operating Instructions 28 9597 89 AA Error symptom Waves on the gradi ent Possible cause Incorrect pump func tion 6 Troubleshooting 6 2 Conductivity curve problems Corrective action Check that the pump is operating and is programmed correctly Dirty mixing chamber Check that the mixing chamber is free from dirt or particles Insufficient mixing chamber volume Change to a larger mixing cham ber volume if necessary Incorrect motor func tion Check the motor operation Place a hand on the mixer and start it by starting the pump at a low flow rate You should both hear and feel the mixer motor and stirrer when they are spinning Ghost peaks appear in the gradient profile Air in the flow cell Check for loose tubing connec tions Use the flow restrictor Unlinear gradients or slow response to B changes Dirty tubing Wash the tubing and check pump is operating properly Incorrect mixer vol ume
29. mponents and liquid containers remove any loose items from the top of the system grasp the system firmly by placing the fingers under the base of the main unit and lift For more information on Transport see AKTAprime plus Installation Guide 26 AkTAprime plus Operating Instructions 28 9597 89 AA 3 Installation 3 3 Unpacking 3 3 Unpacking e Check the equipment for damage before starting assembly and installation e Document any damage and contact your local GE Healthcare representative Remove straps and packing material and stand equipment upright before starting installation 3 4 Connections Communication Connect the system according to the electrical drawings in Appendix A Electrical and communication connections on page 57 Ensure that PrimeView software is installed on the computer Only if PrimeView is used as an evaluation system Flow path All parts and tubing are mounted on the system at delivery Connect a waste tube buffer and sample bottles and optional accessories Electrical power Connect the power cord to a grounded power outlet specified in Section 5 1 Site requirements on page 26 5 5 Spare parts and accessories For correct up to date information on spare parts and accessories visit www gelifesciences com AKTA AKTAprime plus Operating Instructions 28 9597 89 AA 27 4 Operation 4 Operation This chapter provides instructions for the use of AKTAprime plus 4 1 O
30. nspection Check for external or internal leakage Replace channel plate and distribution plate yearly or when required Refer to the relevant valve in struction sheet 5 3 Cleaning Cleaning before maintenance service Before maintenance service is performed the system owner must first clean the system and complete a Decontamination Report Contact GE Healthcare for further information Cleaning In Place All components in the system are designed for ease of CIP After repeated separation cycles contaminating material might progressively build up in the system and on the column This material may not have been removed by the cleaning step described above The nature and degree of contamination depends on the sample and the chromatographic conditions employed These should be considered when designing a cleaning protocol Routine cleaning should be performed at intervals aimed at prevention rather than cleaning the system from growth or contamination WARNING Make sure that the piping system is completely leakage free before performing any CIP on the system Make sure that the process control method for cleaning flushes all possible flow paths in the system After cleaning rinse the entire system with water or suitable liquid until the piping tubing system is completely free from the CIP solution monitors in the system can be used as detectors Do not leave NaOH or other cleaning agents in the system for long peri
31. ods AKTAprime plus Operating Instructions 28 9597 89 AA 41 5 Maintenance 5 3 Cleaning WARNING Hazardous chemicals during maintenance When using hazardous chemicals for system or column cleaning wash the system or columns with a neutral solution in the last phase or step WARNING NaOH is corrosive and therefore dangerous to health When using hazardous chemicals avoid spillage and wear protective glasses and other suitable personal protective equipment See also Section 5 7 Storage on page 44 5 4 Component maintenance Maintenance and preventive replacement of parts of the major components are described in the respective manuals included in the system documentation The system documentation also includes a spare part list to be used to find common spare parts and their code numbers for ordering This list can also be found online at www gelifesciences com AKTA 5 5 Disassembly and assembly of components and consumables The operator must carefully read and understand the instructions supplied for each component before disassembly and assembly of the component When replacing consumables such as tubing and tubing connectors all neccessary safety precautions must be taken Contact your local GE Healthcare representative if further information or help is needed WARNING Disconnect power Always disconnect power from the instrument before replacing any component on the instrument unless stated othe
32. onths Acetaldehyde OK OK Acetic acid lt 5 OK OK Acetic acid 70 OK OK 64 19 7 200 580 7 Acetonitrile OK OK 75 05 8 200 835 2 FFKM PP and PE swell Acetone 10 OK Avoid PVDF is affected by long term use 52 AKTAprime plus Operating Instructions 28 9597 89 AA 7 Reference information 7 2 Chemical resistance Chemical Exposure Exposure Comments lt 1 day up to 2 months Ammonia 30 OK OK 7664 41 7 231 635 3 Silicone is affected by long term use Ammonium chlo OK OK 12125 02 9 235 186 4 ride Ammonium bicar OK OK bonate Ammonium nitrate OK OK Ammonium sul OK OK 7783 20 2 231 984 1 phate 1 Butanol OK OK 2 Butanol OK OK Citric acid OK OK 29340 81 6 249 576 7 Chloroform OK Avoid Kalrez CTFE PP and PE are affected by long term use Cyclohexane OK OK Detergents OK OK Dimethyl sulphox Avoid Avoid 67 68 5 200 664 3 PVDF is affected by ide long term use 1 4 Dioxane Avoid Avoid ETFE PP PE and PVDF are affected by long term use Ethanol 100 OK OK 75 08 1 200 837 3 Ethyl acetate OK Avoid Silicone not resis tant Pressure limit for PEEK decreases Ethylene glycol OK OK 107 21 1 203 473 3 10096 Formic acid 100 OK OK 64 18 6 200 579 1 Silicone not resis tant AKTAprime plus Operating Instructions 28 9597 89 AA 53 7 Reference information 7 2 Chemical resistance
33. orage the system must first be cleaned as described in Cleaning In Place on page 41 After cleaning the system must be filled with 0 01 M NaOH or 2096 ethanol solution Columns and media shall be stored according to their respective instructions Storage conditions The following conditions shall be maintained while the system is in storage Temperature 2 to 30 C preferably room temperature Relative humidity 0 to 95 non condensing preferably low humidity After storage clean the system calibrate all monitors and perform a leakage test before using the system 44 AKTAprime plus Operating Instructions 28 9597 89 AA 6 Troubleshooting 6 Troubleshooting 6 1 UV curve problems Error symptom Ghost peak Possible cause Dirt or residues in the flow path from previ ous runs Air in the eluents Corrective action Clean the system Make sure air is removed Residue in the column from previous runs Clean the column according to the column instructions Incorrect mixer func tion Check the mixer function by plac ing astirrer bar on top of the mixer housing The stirrer bar should ro tate when the system is in Run mode The mixer function can also be checked by running the installa tion test Noisy UV signal sig nal drift or instability Dirty UV cell Clean the UV cell by flushing De cone 90 Deconex 11 or equivalent Impure buffer Check if the signal is stil
34. oving the instrument e System operation e Maintenance General precautions Always follow these General precautions to avoid injury when using the AKTAprime plus instrument WARNING Do not operate the AKTAprime plus instrument in any other way than described in the AKTAprime plus and PrimeView manuals 14 AkTAprime plus Operating Instructions 28 9597 89 AA 2 Safety instructions 2 1 Safety precautions WARNING Operation and user maintenance of the AKTAprime plus instrument should be performed by properly trained personnel only WARNING Before connecting a column to the AKTAprime plus instrument read the instructions for use of the column To avoid exposing the column to excessive pressure make sure that the pressure limit is set to the specified maximum pressure of the column WARNING Do not use any accessories not supplied or recommended by GE Healthcare WARNING Do not use the AKTAprime plus instrument if it is not working properly nor if it has suffered any damage for example gt P gt gt damage to the power cord or its plug e damage caused by dropping the equipment e damage caused by splashing liquid onto it CAUTION Waste tubes and containers shall be secured and sealed to prevent accidental spillage CAUTION Make sure waste container is dimensioned for maximum possible volume when the instrument is left unattended AKTAprime p
35. peration overview Workflow The typical workflow in AKTAprime plus after turning on the system can be divided into a number of steps Step Action Section 1 Prepare the system for a run Section 4 3 Preparations before start on page 29 2 Start a run using a method Section 4 4 Performing a run on page 34 3 During a run view and change parameters Viewing the run on page 35 4 Procedures after a run Section 4 5 Procedures after a run on page 36 5 Evaluate the results See PrimeView user documentation Liquid flow path See Appendix B Connection diagram Liquid flow path on page 58 for an illustration of the liquid flow path in AKTAprime plus 4 2 Starting the instrument If the system is not already turned on 1 Turn on the system using the Power switch at the rear panel The system now performs a self test 2 Firstthesystem name and software version number are displayed Several messages are then shown during the self test If an error is detected during the self test an error message is shown 3 All parameters are automatically set to factory default values during the self test 4 The self test takes about 30 40 seconds When the test is completed the display shows the Templates menu 28 AkTAprime plus Operating Instructions 28 9597 89 AA 4 Operation 4 2 Starting the instrument Note The system can be used for most applications after 15 min of lamp warm up but the full specifications
36. piping system is pressurized Note that the piping system can be pressurized even when the system is closed down for correct installation of a new UV lamp If the lamp is not installed properly it may be overheated and cause a fire hazard NOTICE When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system CAUTION Fire hazard Follow instructions in the AKTAprime plus User Manual NOTICE Cleaning Keep the instrument dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cleaning agent Let the instrument dry completely before use 5 2 User maintenance schedule Table 5 1 provides a guide to maintenance operations and intervals at which these operations should be performed by the user The user is however responsible for deciding the type of operations and length of intervals necessary to maintain system function and safety Table 5 1 User maintenance schedule AKTAprime plus Operating Instructions 28 9597 89 AA 39 5 Maintenance 5 2 User maintenance schedule 40 Interval Action Instructions reference Daily Leak inspection Visually inspect the system for leaks Wash the system flow 4 For cleaning the flow path see Cleaning In path Place on page 41 2 For leaving the s
37. r pH electrode If the response differ greatly the electrode may require cleaning or replacement In organic solvents such as ethanol methanol and acetonitrile stable pH measurements are not possible since dehydration of the membrane will occur It is recom mended that the pH electrode is not used in applications using or ganic solvents Mount the dummy electrode instead Incorrect pump or Check that the pump and valves valve operation operate correctly Air in the flow cell If air in the flow cell is suspected tap the flow cell carefully or tilt it to remove the air Alternatively flush the cell with buffer at 20 ml min E 100 system or 10 ml min E 10 system for 1 2 min Use the flow restrictor FR 902 after the pH electrode Static interference There may be interference from static fields Connect the pH flow cell and the rear panel of the monitor using a standard laborato ry 4 mm banana plug cable AkTAprime plus Operating Instructions 28 9597 89 AA Error symptom Possible cause 6 Troubleshooting 6 3 pH curve problems Corrective action pH values vary with varied back pressure Problem with the electrode Replace the pH electrode Error symptom Erratic flow noisy baseline signal irregu lar pressure trace 6 4 Pressure curve problems Possible cause Air bubbles passing through or trapped in the pump Corrective action Check all connections for leak
38. re monitor G1 PEEK 150 0 75 66 Pressure monitor Mixer G2 PEEK 120 0 75 53 Mixer Valve G3 PEEK 300 0 75 133 Valve UV Column G4 PEEK 650 0 75 287 UV Cond G5 PEEK 250 0 75 110 Cond Flow restrictor Union PEEK 38 0 50 7 1 16 male 1 16 male Flow restrictor Frac coll G6 PEEK 550 0 75 243 Frac tubing F1 PEEK 410 0 75 181 Waste W1 W2 W3 PEEK 1000 1 0 785 AKTAprime plus Operating Instructions 28 9597 89 AA 59 For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com AKTA imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Drop Design HiPrep HisTrap HiTrap PrimeView KTA and KTAprime are trademarks of GE Healthcare companies All third party trademarks are the property of their respective owners 2009 General Electric Company All rights reserved First published Aug 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information PrimeView Any use of this software is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products GE Healthcare Europe GmbH
39. rument operates under high pressure Wear protective glasses at all times Installing and moving the instrument WARNING Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before connecting the power cord WARNING Protective ground The AKTAprime plus instrument must always be connected to a grounded power outlet WARNING Power cord Only use power cords delivered or approved by GE Healthcare AKTAprime plus Operating Instructions 28 9597 89 AA 17 2 Safety instructions 2 1 Safety precautions WARNING Access to power switch and power cord Do not block the rear and side panel of the instrument The Power switch must always be easy to access The power cord must always be easy to disconnect WARNING Installing the computer The computer should be installed and used according to the instructions provided by the manufacturer of the computer NOTICE Disconnect power To prevent equipment damage always disconnect power from the KTAprime plus instrument before an instrument module is removed or installed or a cable is connected or disconnected System operation WARNING Hazardous chemicals during run When using hazardous chemicals run System CIP and Column CIP to flush the entire system tubing with distilled water before service and maintenance WARNING Hazardous
40. ruments or e connected to other products recommended or described in the user documentation and e usedinthe same state as it was delivered from GE Healthcare except for alterations described in the user documentation The Declaration of conformity is valid only for systems that are marked with the CE marking CE Regulatory compliance of connected equipment Any equipment connected to the AKTAprime plus system should meet the safety requirements of EN 61010 1 IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be CE marked AKTAprime plus Operating Instructions 28 9597 89 AA 1 Introduction 1 3 Instrument 1 3 Instrument Product description AKTAprime plus is a compact liquid chromatography system designed for one step purification of proteins at laboratory scale 17 1 r ne 16 2 4 5 Figure 1 1 The main parts of the instrument Part Function Part Function 1 Fraction collector 10 Switch valve 2 Monitor and controller 11 Conductivity cell 3 LCD display 12 Flow restrictor 4 Push buttons 13 UV flow cell 5 Pump 14 Column 6 Pressure sensor 15 Sample loop iC Mixer 16 Flow diversion valve 8 Injection valve 17 Column holder 10 AkTAprime plus Operating Instructions 28 9597 89 AA 1 Introduction 1 3 Instrument Part Function Part Function 9 Buffer valve The Power switch is located at
41. rwise in the user documentation 42 AkTAprime plus Operating Instructions 28 9597 89 AA 5 Maintenance 5 5 Disassembly and assembly of components and consumables WARNING Before disassembly check that there is no pressure in the piping system WARNING After assembly the piping system must be tested for leakage at maximum pressure for continued protection against injury risks due to fluid jets burst pipes or explosive atmosphere 5 6 Calibration The table below lists the type and frequency of calibrations that can be done on the instrument Refer to PrimeView user documentation and to the individual component User Manuals and Instructions for descriptions of how to perform these calibrations The calibrations are performed from PrimeView by selecting System Calibrate in System Control Component How often pH monitor if applicable Every day Pump if applicable When required Pressure reading When required Conductivity flow cell Cell constant Only necessary if specific conductivity with high accuracy is measured Cond_Calib Temperature Must be done when changing the conductivity flow cell Temp Entering a Must be done when changing the conductivity new cellcon flow cell Cond Cell stant AKTAprime plus Operating Instructions 28 9597 89 AA 43 5 Maintenance 5 7 Storage 5 Storage General recommendation For st
42. s Check that there is sufficient eluent present in the reservoirs Use degassed solutions Purge the pump Follow the instructions in AK TAprime plus User Manual Inlet or outlet check valves not functioning correctly Clean the valves in according to AKTAprime plus User Manual Piston seal leaking Replace the piston seal according to the instructions in AKTAprime plus User Manual Blockage or part blockage of flowpath Flush through to clear blockage If necessary replace tubing Check inlet tubing filter It can be come clogged if unfiltered buffers or samples are applied See instruc tions for flushing through at the end of the run in AKTAprime plus User Manual AKTAprime plus Operating Instructions 28 9597 89 AA 51 7 Reference information 7 Reference information This chapter contains technical data regulatory and other information 7 1 Specifications Parameter Value Ingression protection Housing IP20 Flow cells IP44 Supply voltage 100 120 220 240 V 50 to 60 Hz Power consumption 90 VA Fuse specification T 1 0 AH 250 V Dimensions H x W x D 530 x 400 x 450 mm Weight 13 kg Ambient temperature 4 to 40 C Relative humidity tolerance non condensing 10 to 95 Atmospheric pressure 84 to 106 kPa 840 to 1060 mbar 7 2 Chemical resistance Chemical Exposure Exposure CAS no Comments lt 1 day upto2 m
43. s EN E 6 1 AV CUPVE problems etate ett o tr ERR 62 Conductivity curve problems ee tecti rtr n E ERR ERR 6 3 pH curve problems s 6 4 Pressure curve problems in PRTC SMU UMNO nm ne VAST Telle Dac c AKTAprime plus Operating Instructions 28 9597 89 AA Table of Contents 42 Chemical r sistance nn nan rennes 52 7 3 System recommendations 55 7 4 Literature sss 56 25 Ordering informations rii ener e noe rre EAT DR DUREE N 56 A Electrical and communication connections eese 57 B Connection diagram Liquid flow Dotnet 58 MIT nan dd nn ann nus 59 4 AKTAprime plus Operating Instructions 28 9597 89 AA 1 Introduction 1 Introduction Purpose of the Operating Instructions The Operating Instructions provides you with the instructions needed to handle the AKTAprime plus system in a safe way Prerequisites In order to operate the system in the way it is intended the following pre requisites must be fulfilled e You should have a general understanding of how the PC and Windows works if a computer is used e You should understand the concepts of liquid chromatography You must read and understand the Safety Instructions e The instrument and software should be installed configured and calibrated according to the Installation Guide In this chapter This chapter contains important user information and a general description of the AKTAprime plus system and its intended use
44. trument XX XXXX XX O xxxxx Sample Code No XXXXXXXX Voltage Serial No XXXXXXX Frequency Mfg Year 2009 Power max Fuse AN Qoy C C TELLE MIA Made in Sweden GE Healthcare Bio Sciences AB en 751 84 Uppsala Sweden 36 J Symbols used in safety labels Label Meaning Warning Read the user documentation before using the system Do not N open any covers or replace parts unless specifically stated in the user documentation The system complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand The system complies with applicable European directives 22 AkTAprime plus Operating Instructions 28 9597 89 AA 2 Safety instructions 2 2 Labels Labels concerning hazardous substances Label Meaning This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufac mummm turer for information concerning the decommissioning of equipment This symbol indicates that the product contains hazardous materials in Lay excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronics 2 3 Emergency procedures This section describes how to do an emergency shutdown of the AKTAprime plus system
45. ystem for a few days see Section 5 7 Storage on page 44 Calibrate pH electrode Calibrate the pH electrode if applicable accord optional ing to Monitor pH C 900 User Manual Weekly Check inlet filters Check the inlet filters visually and replace them if necessary Replace on line filter Replace the on line filter if applicable Monthly Flow restrictor Check that flow restrictor generates the follow ing back pressure FR 904 0 4 0 05 MPa FR 902 0 2 0 05 MPa Check the back pressure as follows 1 Disconnect the flow restrictor 2 Connect a capillary approx 1 m i d 1 mm to the waste port port 5 on the injection valve Set the injection valve manually to Waste position Put the open end in a waste container 3 Runthe pump manually at 10 ml min with water Note the back pressure Bp1 on the pump display or in the Run Data window 4 Set the system to Pause and connect the flow restrictor to the open end of the capil lary lobserve the IN marking Put the flow restrictor in the waste container 5 Press Continue so that the pump run at 10 ml min with water Note the back pressure Bp2 on the pump display or in the Run Data window 6 Calculate the back pressure generated by the flow restrictor Bp2 Bp1 Replace it if it is not within limit AkTAprime plus Operating Instructions 28 9597 89 AA 5 Maintenance 5 2 User maintenance schedule Interval Instructions reference Yearly Valve i
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