KingFisher Duo Prime Brief User Manual
Contents
1. Navigating This section visualizes navigation in the KingFisher Duo Prime user interface wv n The main view changes according to the selections you make either with the Up or Down arrow keys or the OK button To move into the submenus use the OK button The available buttons and their function are shown on the info text bar The color of the items for example the icon and main view row in the main view changes when they are selected active inactive To move from one menu to another make sure you are in the main view of one of the menus and use 4 b the Left and Right arrovv keys To return from the submenus to the main vievvs use the Left arrovv key The main views of each menu tab are shown in the following figure 14 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific 3 Operation otarting KingFisher Duo Prime 09 04 E 2 4 09 09am s V DNA 4 Run Log 27 Q UV Lamp m RNA EA KF BloodDNA Duo Export Device Report Protein 44 19 01 2015 08 28 am al Export Run Log A Other b b ke Import Protocol MW DNA RNA Export Protocol ky Protein Maintenance Protocols Cell lt lt Information Other Q Language EKF BloodDNA Duo Buzzer 2 Date and Time Barcode Reader Select Start 4 Select Details v Select Ed Details Ed UV Lamp Figure 9 Main views of menu tabs Starting KingFisher Duo Prime You can have2. KingFisher Duo Prime Brief User Manual Rev 1 0 Cat no N16622 2015 Thermo Fisher Scientific Inc All rights reserved KingFisher Duo Prime magnetic particle processor and BindIt Software are registered trademarks of Thermo Fisher Scientific Inc Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history
3. March 2015 First release for KingFisher Duo Prime For Research Use Only Not for use in diagnostic procedures WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equip ment WEEE Directive 2002 96 EC It is marked with the following symbol T in Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product Contact Thermo for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country Contents Uhapter 1 Uhapter 2 Uhapter 3 Uhapter 4 Thermo Fisher Scientific Biri aa n Vil About This Guide 4244009 04 6 Da dur x ob R mm E a vii i RP TUTTI vii Is P MER vii Introduction to KingFisher Duo Prime 1 Intended U Se 2 vik a sospes ded cook Bed OE RCR RUE 7 1 bada 3900099002 sean vetoes 1 INSTA AUOM MEET 3 Unpacking PQuIpMGNt 4 eura 4 Removing Internal dd 5 Releasing Transport Lock r ree PPS Ue Pee Es ERE Pura 6 Connecting Power Supply Cable 22 2 yer Raz Ba a Ras rds 7 Mall PDT 8 lnserting Heating lori cese one ao baa eevee as ti AY 8 Inserting
4. Thermo Scientific User Awareness of Symbols Declaration of Conformity document Note It is recommended to save the user manuals on the installation CD as backup files on your PC Note Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so might cause misalignment and will void the instrument warranty CAUTION The KingFisher Duo Prime weighs approximately 17 kg 37 5 lbs without the transport package and must be lifted with care It is recommended that two persons lift the instrument together taking the proper precautions to avoid injury To lift the instrument place your fingers under the bottom of the device on both sides and lift it with your back straight 4 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific 2 nstallation Unpacking Equipment Removing Internal Cushions When you have opened the package open the front lid of the instrument and remove the two internal cushions First remove the larger cushion in the middle and then remove the smaller cushion which protects the UV lamp To remove the smaller cushion carefully pull it outwards and then down For illustrations see the following figures Figure 2 Removing internal cushions Bs Figure 3 Removing the UV lamp protection Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 5 2 Installation Releasing Transport Loc
5. To install the Bindlt software Insert the BindIt Software installation CD into the CD ROM drive of your PC The Bindlt Installer dialog opens automatically If the dialog does not open start the installation from the CD by double clicking the Setup exe file Close all other programs on your computer and click Install to begin the installation process To cancel the installation and exit the installer click Close Note If you have an existing instance of Microsoft SQL Server 2008 R2 Express Edition on your computer you can choose to use the existing instance or create a new one If you are uncertain of the compatibility create a new instance by clicking Yes If you do not wish to create a new instance click No Note The installation of a new database engine does not affect any other installed database engines oF To start the Setup Wizard click Install The wizard guides you through the installation procedure To continue click Next Read the end user license agreement and tick the selection box to accept the terms Click Next to continue e To print a copy of the license agreement click Print Select the destination folder for the BindIt Software installation files The Setup Wizard suggests a location for the files Using the default file location is recommended e To select another folder or drive click Change e Click Next to continue The Setup Wizard is now ready to install the Bin
6. two runs ongoing at the same time To start the instrument 1 Select a protocol by using the arrow keys and press START or use the BindIt Software to run the desired protocol on the PC If you have the 2D Barcode reader for KingFisher N16640 connected see Figure 1 on page 2 use the barcode reader to read the barcode of consumables and or samples To check the barcode reader status select the Maintenance menu and then click the Barcode Reader row Use the Up and Down arrows to select the wanted row and to read the respective barcode Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 15 3 Operation otarting KingFisher Duo Prime A bl 7 03 20pm kl KF BloodDNA Duo 044896001798661016 ing 434910 1432810 5 416312 Press W to continue Read barcodes max 1 s v Select Q Stop F Figure 10 Using the barcode reader to read the barcodes 2 Press the OK button 3 Make sure that the front lid is open 4 Load the plate s and elution strip s in the order that the protocol requests e Place the plate into the device so that the A1 corner is in the inner circle in the upper right corner see Figure 11 on page 17 e Use the elution strip holder to hold the elution strip in place illustrated in Figure 11 on page 17 There is a tap that helps placing the elution strip correctly see the following figure 16 Thermo Scientific KingFisher Duo Prime Brief User Manual Th
7. Magnet Head ies edeserteteshoteRIREPQUATAREIQSATAARE ED 8 d MT 9 Installing BindIt Software llle 10 Importing Protocols Using USB Memory Device 11 JI 13 13 ET 14 Starting KingFisher Duo PEIUS S es 15 Switching UV Lampa bra 18 Technical Specifications 19 General Specie ONS es oscar duque ee bt pede pu ERE IG daa da don 19 5410077 ii li ab eant lo po ee pbs 0 aes 19 Thermo Scientific KingFisher Duo Prime Brief User Manual V Contents vi Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific Preface About This Guide This Brief User Guide is intended for the Thermo Scientific KingFisher Duo Prime instrument It TM aims to give you a short introduction on installing the instrument and Thermo Scientific BindIt Software as well as starting a purification protocol from the internal software The complete user manual for the KingFisher Duo Prime instrument is available on the KingFisher User Manuals CD For instructions on the BindIt Software see the BindIt Software for KingFisher Instruments CD Read the user manuals in their entirety before operating the instrument For details and ordering information on plastic consumables such as plates tip combs e
8. ative humidity at 40 C For indoor use only 100 240 Vac 50 60 Hz nominal Automatic voltage detection 96 VA max 10 VA standby Safety specifications are in conformity with the requirements In addition to or in excess of those stated in the operating conditions the safety specifications are also met under the environmental conditions listed in the following table Table 3 Safety specifications Condition Altitude Temperature Humidity Mains supply fluctuations Thermo Fisher Scientific Value Up to 2000 m 4 C to 40 C Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C 10 from nominal Thermo Scientific KingFisher Duo Prime Brief User Manual 19 4 Technical Specifications Safety Specifications Table 3 Safety specifications Condition Value Installation category II according to IEC 60664 1 see Note 1 overvoltage category Pollution degree 2 according to IEC 60664 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories th
9. dIt Software on your computer Click Install to start the installation The installation files are copied to the selected folder The installation progress dialog opens The BindIt Software has now been installed Click Finish to finalize the installation and to configure the database 10 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific 2 Installation Importing Protocols Using USB Memory Device e The BindIt Installer continues by installing the database engine If existing SQL server instances are detected on the computer click Yes to install a new instance or No to use an existing instance Note Creating several concurrent SQL server instances may slow down your computer We recommend using an existing THERMO instance if available f no previous SQL server instances are detected Microsoft SQL Server 2008 R2 Express Edition is installed on to your computer Installing a new instance of the SOL server takes some time 8 Check the server and database names in the Database Configuration dialog 9 Click Configure The installation proceeds with the database configuration Wait until it has finished This may take several minutes 10 When the installation is complete a confirmation message is shown Click OK to complete the installation process Importing Protocols Using USB Memory Device You can import a protocol into the KingFisher Duo Prime using a USB memory device No
10. e expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply Note 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation 20 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific
11. e the process The magnets move on top of the shield plate at the end of the changing process 6 Close the maintenance door by lifting it up to and push it to its slot When you change the magnet head take away the magnet head by turning the two finger screws on top of the magnet head clockwise When you remove the KingFisher Duo Prime head be careful not to damage the magnet rods against the tip comb holder frame Note When changing the magnet head run the Change magnet head protocol under Maintenance Operational Check To verify proper instrument operation it is recommended that you carry out a check run using a maintenance protocol Run the check protocol Check 12 rip or Check 6 tip according to the magnet head under the Maintenance menu If there are no problems proceed with your own runs Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 9 2 installation Installing Bindlt Software Installing Bindlt Software Start the installation of the BindIt software by following the instructions given in this section For detailed instructions on installing and using the BindIt Software see the BindIt Software for KingFisher Instruments User Manual Cat No N07974 Note the following before you start installing the software Bindlt Software cannot be installed on a network drive To be able to install the BindIt Software you must be logged on to your computer with administrator rights
12. ermo Fisher Scientific 3 Operation otarting KingFisher Duo Prime Figure 11 Loading the plate and the elution strip 5 When you have loaded the requested plates into their respective plate positions press the OK button The instrument functions with either one or two plates depending on the number of steps The tip comb always has to be placed manually onto the KingFisher DW plate row B see the previous figure Only one tip comb can be placed onto a KingFisher plate during one run Note When the instrument is in its basic position plate position 1 is under the Thermo Scientific KingFisher Duo Prime head At the end of a protocol run the turntable may stop in a different position than the starting position e The tip comb automatically locks onto the tip comb holder from the plate e When the turntable rotates the magnet head moves on top of the fixed shield plate that forms a protective cover 6 Close the front lid The front lid protects the instrument against environmental contamination Note You can leave the front lid open if desired This action does not break the run Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 17 3 Operation otarting KingFisher Duo Prime 7 When the run is finished remove the plate s and strip s according to the protocol request CAUTION Remove any plates strips or tip combs still in the instrument Dispose of all microplates
13. isher Duo Prime magnetic particle processor is intended for professional research use by trained personnel The instrument is intended for automated transfer and processing of magnetic particles in a microplate format Use for self testing is excluded It is recommended that Good Laboratory Practice GLP is followed to guarantee reliable analyses Operation Principle The patented technology of the KingFisher Duo Prime system is based on the use of magnetic rods covered with a disposable specially designed tip comb and plates The instrument functions without any dispensing or aspiration parts or devices Samples and reagents including magnetic particles are dispensed into the plates according to the corresponding instructions The protocol that is selected by the user with the keypad and display has already been transferred onto the onboard software BindIt Software can be used to create and run protocols Figure 1 illustrates the KingFisher Duo Prime instrument and its parts listed in the table that follows Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 1 1 Introduction to KingFisher Duo Prime Operation Principle Figure 1 KingFisher Duo Prime magnetic particle processor Item Item Item Item number number 1 Maintenance door 6 Front lid closed USB port also at the back of the device Cooling air outlet 8 On off switch 4 Display 9 UV lamp 5 Keypad 10 USB port and barcode reader connecti
14. k Releas ng Transport Lock The instrument comes with one transport lock Release the transport lock before you put the instrument into operation To release the transport lock unscrew the transport lock finger screw with spring suspension counterclockwise see the following figure T he loosened finger screw stays in the same location by spring force It is possible to use a suitable tool such as a screwdriver or a coin to unscrew the transport lock Figure 44 Releasing the transport lock 6 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific 2 Installation Connecting Power Supply Cable Connecting Power Supply Cable WARNING Make sure that the main switch see point 8 in Figure 1 on page 2 on the front panel is in the OFF position Never operate your instrument from a power outlet that has no ground connection Never use a power supply cable other than the Thermo Scientific power supply cable designed for your region To connect power supply cable 1 Connect the power supply cable to the power supply connector and plug in the instrument see the following figure EY WU N 7 e x 44 Figure b Povver supply cable connected 2 Connect the power supply to a correctly installed line power outlet with a grounded conductor Turn the power switch to the ON position to perform the next task The instrument performs initialization tests and adjust
15. lution strips and such used with the KingFisher Duo Prime instrument see the List of accessories and consumables in the Thermo Scientific KingFisher Duo Prime Technical Manual Cat no N16621 Reagents There is a wide selection of optimized Thermo Scientific KingFisher Pure Kits available for purification of DNA or RNA A large variety of starting materials can be used such as blood cells tissues or cell free body fluids The DNA or RNA purified with the KingFisher Pure Kits is of high quality and free of proteins nucleases and other contaminants or inhibitors For more information see the list of KingFisher Pure Kits in the KingFisher Duo Prime Technical Manual Thermo Scientific Pierce Protein Research Products are available for protein applications For more information on available kits visit www thermoscientific com pierce The KingFisher Duo Prime is an open system vvhich also enables the use of other magnetic particle kits The optimal magnetic particle size for the KingFisher Duo Prime is approximately 0 8 10 um Contacting Us For the latest information on products and services visit our website at http www thermoscientific com kingfisher Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual vii Preface viii Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific M Introduction to KingFisher Duo Prime Intended Use The KingF
16. me head in close proximity to magnetic tapes computer discs or other magnetic storage systems such as credit cards as these can be damaged by the strong magnetic field of the KingFisher Duo Prime heads Do not hold the KingFisher Duo Prime heads close to a PC display as this may cause damage to the display Do not use metal tools when handling the KingFisher Duo Prime heads WARNING Risk of burns The heating block and or the elution block surface can be hot The warning lights are lit if the temperature rises above 50 C Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 3 2 Installation Unpacking Equlpment Unpacking Equipment Move the packed instrument to its site of operation To prevent condensation leave the instrument in its protective antistatic plastic wrapping until the ambient temperature has been reached Unpack the KingFisher Duo Prime instrument and accessories carefully with the arrows on the transport package pointing upwards Remove the instrument from the package and place it on a level surface The following notes and instructions are sent with the instrument and are immediately available when you open the package Bindlt Software installation CD including Thermo Scientific BindIt Software for KingFisher Instruments User Manual Manuals CD containing the following documents Brief User Manual this manual with translations e KingFisher Duo Prime Technical Manual e
17. ments Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 7 2 Installation oetting Up Setting Up Before running KingFisher Duo Prime you need to set up the instrument by inserting heating blocks and the magnet head Inserting Heating Blocks To insert the heating blocks 1 Insert the first heating block according to the used plate type Set it into the plate position and snap it into place see the following figure Figure 6 Inserting the heating block 2 Press the ROTATE button to get the other heating block in the front 3 Insert the second new heating block by setting it and snapping it into place 4 To change the heating blocks take away the heating block by lifting it and repeat Steps 1 3 Inserting Magnet Head To insert the magnet head 1 Open the maintenance door by lifting it up and turning it to the front 2 To fit the magnet head choose the Change magnet head protocol under the Maintenance menu using the Up and Down arrow keys Follow the instructions shown on the screen 3 Press the START key The procedure stops to a pause 8 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific 2 Installation Operational Check 4 nsert the magnet head by slotting it into the tip comb holder and by fitting the two finger screws on top of the magnet head see the following figure Figure 7 nserting the magnet head 5 Continu
18. on preferred location To keep the instrument in the best condition for maximum reliability follow the preventative maintenance instructions provided in KingFisher Duo Prime Technical Manual A poorly maintained instrument does not give the best results 2 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific Installation This chapter briefly describes the installation of the KingFisher Duo Prime instrument WARNING This product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact 10 cm or 3 9 inches with a strong magnetic field CAUTION The heating blocks are specifically designed for the plastic consumables listed to ensure even heating during the sample process Using other plastics than those recommended may damage the instrument diminish the application performance and cause cross contamination due to the divergent well volume and bottom height of the plate CAUTION Do not place the KingFisher Duo Prime heads on any metal surfaces Keep the KingFisher Duo Prime heads always in their respective plastic boxes when not in use It is very important to keep the KingFisher Duo Prime heads away from each other and other magnets at all times Clashing of the magnets together may cause serious damage to the magnets CAUTION Do not keep the KingFisher Duo Pri
19. strips and tip combs as biohazardous waste Tip You can start another run normally while the previous run is ongoing Switching UV Lamp On You can use the UV lamp to decontaminate the process chamber If you open the front lid the UV lamp shuts down Note that UV treatment is not a substitute for cleaning the instrument 1 Hr CO N To switch on the UV lamp Go to the Maintenance menu Select the UV Lamp row and press the OK button To set the time of the UV lamp select the Set time row and press the OK button Use the Up and Down arrows to set the wanted duration To accept the setting and to move to the minute setting press the OK button You can set the duration in hours between 00 and 16 and the duration in minutes between 00 and 59 When you open the setting the time shown is the previously set time for the UV lamp usage The default time is 00 30 18 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific Technical Specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program General Specifications Table 2 General specifications Condition Operating conditions indoor use Mains power supply Power consumption Safety Specifications Value 4 C to 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 rel
20. te If you export device reports run logs and or protocols to a blank USB device the exported items go to the KingFisher Duo Prime folder which is automatically created on the USB memory device If you import protocols from the USB memory device to the instrument they must already be in the correct folder named KingFisher Duo Prime Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 11 2 installation mporting Protocols Using USB Memory Device 12 Thermo Scientific KingFisher Duo Prime Brief User Manual Thermo Fisher Scientific Operation Keypad The keys and control buttons are shown in the following figure and described in Table 1 KINGFISHER DUO PRIME Figure 8 KingFisher Duo Prime keypad Table 1 Keys and control buttons Key Description Use the Up Down Left and Right arrow keys to select a protocol and to navigate on the display A 4 Use the OK button to accept the selection and to confirm a 22 performed step in the protocol for example plate loading or removal Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Brief User Manual 13 3 Operation Navigating Table 1 Keys and control buttons Key Description Use the Start button to initiate the run Use the Stop button to terminate the protocol s Use the Pause button to stop the run The run pauses at the end of the ongoing processing step Use the Rotate button to rotate the turntable
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