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1200 Infinity Series Variable Wavelength Detectors User Manual

1. s a yan Nea DEFAULT D Last Run Snares q Method DEF_LC M aa Sequence DEF_LC S EMF io z mid omg Ma ALTA O AORA ret noDetals DEFAULT D E Adata E 1 1 GB free Figure 18 Turning on the HPLC Module 62 Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 5 Purge the pump For more information Priming and Purging the System on page 57 6 Allow the detector to warm up of at least 60 minutes to provide a stable baseline example Figure 19 on page 63 For reproducible chromatography the detector and lamp should be on for at least one hour Otherwise the detector baseline may still drift depending on the environment MAJ heater control is OFF heater control is ON Figure 19 Stabilization of baseline after turn on of detector ming 7 For the isocratic pump fill the solvent bottle with the mixture of HPLC grade bi distilled water 80 and acetonitrile 70 For binary and quaternary pumps you can use separate bottles Agilent 1200 Infinity Series VWD User Manual 63 4 Using the Detector Setting up an Analysis 8 Click on the Load Method button select DEF_LC M and press OK Alternatively double click on the method in the method window The default LC method parameters are transferred into the modules ax forty chm DEF_LC M e chem32 2 methods D gt
2. HMM WN Solvent cabinet Degasser optional Autosampler ALS Fraction collector Thermostat for the ALS Fraction collector optional Figure 9 Recommended Two Stack Configuration for 1260 Infinity Front View Agilent 1200 Infinity Series VWD User Manual y 3 Installing the Detector Optimizing the Stack Configuration LAN to control software RAN Bus cable to Instant Pilot utosampler Fraction Cu lector C Il fu BELLU Remote cable Figure 10 Recommended Two Stack Configuration for 1260 Infinity Rear View 42 Agilent 1200 Infinity Series VWD User Manual Installing the Detector 3 Optimizing the Stack Configuration Two Stack Configuration for Agilent 1290 Infinity LC In case the autosampler thermostat is added to the system a two stack configuration is recommended which places both heavy modules 1290 Infinity pump and thermostat at the bottom of each stack and avoids high stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 11 on page 43 and Figure 12 on page 44 Instant Pilot Detector Column compartment J l J 1 Solvent cabinet Pump Al ce ee j d q Autosampler Thermostat for the ALS optional Fi
3. Detector Introduction to the Detector 10 Optical System Overview 11 Flow Cell 12 Lamp 13 Source Lens Assembly 13 Entrance SlitAssembly 13 Filter Assembly 14 Mirror Assemblies M1 and M2 15 Grating Assembly 15 Beam Splitter Assembly 15 Photo Diodes Assemblies 15 Photo Diode ADC analog to digital converter 15 System Overview 16 Leak and Waste Handling 16 This chapter gives an introduction to the detector instrument overview and internal connectors ott Agilent Technologies 9 1 Introduction to the Variable Wavelength Detector Introduction to the Detector The Agilent variable wavelength detectors described in this manual are designed for highest optical performance GLP compliance and easy maintenance with data rate up to 20 Hz for standard HPLC G1314D see Table 10 on page 78 higher data rate up to 80 Hz for fast HPLC G1314F see Table 11 on page 78 higher data rate up to 160 Hz for ultra fast HPLC G1314E see Table 12 on page 79 data recovery card DRC G1314E gives unique data never lost insurance Run Recovery Settings on page 80 deuterium lamp for highest intensity and lowest detection limit over a wavelength range of 190 to 600 nm optional flow cell cartridges standard 10 mm 14 uL high pressure 10 mm 14 uL micro 3 mm 2 uL semi micro 6 mm 5 uL are available and can be used depending on the application needs other types may be introduced later easy front access to lamp an
4. Notices Agilent Technologies Inc 2008 2010 2012 2013 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61314 90034 Rev B Edition 11 2013 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written a
5. Create Tab File Edit BootP Addresses Logging BootP Log File ESS ad Sa REG Default Settings Subnet mask GO 0 0 0 Gateway GO 0 0 0 Cancel Help Figure 68 BootP Settings screen 12 In the Default Settings part of the screen if known you can enter the subnet mask and gateway Defaults can be used The default subnet mask is 255 255 255 0 The default gateway is 192 168 254 11 13 On the BootP Settings screen click OK The Agilent BootP Service Setup screen indicates completion 14 Click Finish to exit the Agilent BootP Service Setup screen 15 Remove the DVD from the drive This completes installation 16 Start BootP Service in the Windows services On the Windows desktop click right on Computer icon select Manage gt Services and Applications gt Services Select the Agilent BootP Service and click Start 212 Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Two Methods to Determine the MAC Address Enabling logging to discover the MAC address using BootP If you want to see the MAC address select the Do you want to log BootP requests check box 1 Open BootP Settings from Start gt All Programs gt Agilent BootP Service gt EditBootPSettings 2 In BootP Settings check Do you want to log BootP requests to enable logging Logging V Do you want to log bootP requests BootP Log File Ea TE Figure 69 Enable BootP logging The log file i
6. 3 Click Edit BootP Addresses The Edit BootP Addresses screen appears 4 Click Add The Add BootP Entry screen appears x Mac Address Host Name a IP Address Figure 70 Enable BootP logging 5 Make these entries for the instrument e MAC address e Host name Enter a Hostname of your choice The Host Name must begin with alpha characters i e LC1260 e IP address e Comment optional Agilent 1200 Infinity Series VWD User Manual 215 13 LAN Configuration 216 oo N O e Subnet mask e Gateway address optional The configuration information entered is saved in the Tab File Click OK Leave Edit BootP Addresses by pressing Close Exit BootP Settings by pressing OK After each modification of the BootP settings G e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 217 or Restarting the Agilent BootP Service on page 218 10 Power cycle the Instrument OR If you changed the IP address power cycle the instrument for the changes to take effect 11 Use the PING utility to verify connectivity by opening a command window and typing Ping 192 168 254 11 for example The Tab File is located at C Documents and Settings All Users Application Data Agilent BootP TabFile Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Changing the IP Address of an Instrument
7. Appendix 14 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Agilent 1200 Infinity Series VWD User Manual 237 Index Index 8 bit configuration switch on board LAN 189 A absorbance Lambert Beer 86 Agilent Lab Advisor software 94 Agilent Lab Advisor 94 Agilent oninternet 237 User Interface Software Setup 225 algea information 234 ambient non operating temperature 23 ambient operating temperature 23 analog out put 31 28 25 analog signal 186 analog cable 166 output range 75 output settings 75 apgremote 187 ASTM Drift 122 automatic configuration with Bootp 209 band width 6 5nm 24 27 30 BCD cable 171 beam splitter 15 Beer Lambert absorbance 86 238 bench space 22 boards photodiode boards ADC 15 BootP service installation 211 restart 218 settings 218 stop 217 Bootp amp Store 202 automatic configuration 209 initialization modes 201 using default 203 using stored 203 C cable analog 166 BCD 171 CAN 173 connecting APG remote 42 connecting CAN 42 connecting LAN 42 connecting the ChemStation 42 connecting the power 42 LAN 173 remote 168 RS 232 174 cables analog 164 BCD 164 CAN 165 LAN 165 overview 164 remote 164 RS 232 165 CAN cable 173 cautions and warnings 131 celltest 118 chromatogram 60 cleaning 134 communications 25 28 31 compe
8. If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 23 Forced Cold Start Settings On board LAN Mode Select swi sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 0 0 0 1 192 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself Agilent 1200 Infinity Series VWD User Manual 193 12 Hardware Infor
9. Test 0 Failed Filter motor Test 1 Failed Grating motor During the motor tests the detector moves the motor to the end position while monitoring the end position sensor If the end position is not found the error message is generated Probable cause Suggested actions 1 Motor is not connected Please contact your Agilent service representative 2 Defective motor Please contact your Agilent service representative 3 Defective missing grating or filter Please contact your Agilent service representative 4 Cable connector defective Please contact your Agilent service representative 108 Agilent 1200 Infinity Series VWD User Manual Error Information 7 Wavelength test failed Error ID 7890 The automatic wavelength check after lamp ignition has failed When the lamp is switched on the detector waits 1 min to warm up the lamp Then a check of the deuterium emission line 656 nm via the reference diode is performed If the emission line is more than 3 nm away from 656 nm the error message is generated Probable cause Suggested actions 1 Calibration incorrect Recalibrate the detector Cutoff filter doesn t decrease the light intensity at 250 nm Error ID 7813 The automatic filter check after lamp ignition has failed When the lamp is switched on the detector moves the cutoff filter into the light path If the filter is functioning correctly a decrease in lamp intensity is seen If the expected intensity de
10. Using the Detector 4 Online Spectra 1 To view the online spectra select Online Spectra This online spectrum is taken during a stop flow condition only while the peak is kept in the flow cell see Scanning with the VWD on page 74 E Set up VWD Signal 8 ywo scan y lo x of Control mAU fy Online Spectra S Not Ready Information 200 J Help i 800 400 200 lj ij f r ij ij j f 200 225 250 275 300 325 350 375 nm 100 00 to 1000 0 mau Bal f190 f to nm Figure 29 Online Spectra Window 2 Change the absorbance and wavelength range according your needs Agilent 1200 Infinity Series VWD User Manual 73 4 Using the Detector Scanning with the VWD Access to the scan feature is only possible during run 1 Set up a run Start a run 3 While running on the baseline select from the menu Instrument gt More VWD gt Blank Scan A background scan is stored in the memory System 2 online Method amp Run Control e seod Sequence View EES We Step 1 Blank Scan scan of the background solvent is stored in the memory rn 4 Set up WD Signals a pusi E E Step 2 Sample Scan scan of the peak of interest is Gerik taken while the peak stays in the flow cell stop flow Snapshot condition f fh s i n EKE C CHEMS NOA Online Spectrum Sample Scan minus Blank Scan ab AR CDE System Off i BATCH Sw a DEF_L Revisions amp
11. See also Declaration of Conformity for HOX2 Filter on page 236 When to do the Test e after recalibration as part of the Operational Qualification Performance Verification procedure or after flow cell maintenance or repair Interpreting the Results The test is passed successfully when all three wavelengths are within 1 nm of the expected value This indicates the detector is calibrated correctly Agilent 1200 Infinity Series VWD User Manual Test Functions 8 Holmium Oxide Test Holmium Oxide Test with Agilent Lab Advisor Test Name Holmium Oxide Test Description The test measures the Holmium spectrum from the built in Holmium filter The spectrum is evaluated for peaks at different Module G1314E DE81960002 wavelengths Status Passed Start Time 5 28 2010 10 28 23 AM Stop Time 5 28 2010 10 31 45 AM m Test Procedure Result A 1 Check Prerequisites Accumulated UY Lamp Burn Time 1395 27 h A 2 Perform Holmium Oxide Test UY Lamp On Time O 63h WY 3 Evaluate Data Holmium Deviation to 360 8 nm 000mm Holmium Deviation to 418 5 nm 050nm Holmium Deviation to 536 4 nm Absorbance AU 4 556 4 3 2 1 0 058996 EIF iJ I 1 i 300 400 500 800 Wavelength nm 190 Figure 45 Holmium Oxide Test with Agilent Lab Advisor Holmium Oxide Test Failed Probable cause Suggested actions 1 Detector not calibrated Recalibrate the detector 2 Dirty or defective flow cel
12. G1330B K1330B Yes Yes No THERMOSTAT for G1330B K1330B CAN DC OUT for CAN slaves Yes Yes No Yes 182 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Table 18 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1321C FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed Agilent 1200 Infinity Series VWD User Manual 183 12 Hardware Information Table 18 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G422
13. One Stack Configuration Solvent cabinet Vacuum degasser Pump Instant Pilot Autosampler One Stack Configuration for Agilent 1260 Infinity LC Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration See Figure 5 on page 37 and Figure 6 on page 38 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required Column compartment Detector mni oo 888 Figure 5 Recommended Stack Configuration for 1260 Infinity Front View Agilent 1200 Infinity Series VWD User Manual 37 3 Installing the Detector Optimizing the Stack Configuration INU MAINI PURE TT Remote cable CAN Bus cable to Instant Pilot AC power CAN Bus cable Analog detector signal 1 or 2 outputs portarian LAN to LC ChemStation location depends on detector Figure 6 Recommended Stack Configuration for 1260 Infinity Rear View 38 Agilent 1200 Infinity Series VWD User Manual Instant Pilot Solvent cabinet Detector Column compartment Autosampler Pump Installing the Detector Configuration Optimizing the St One Stack Configuration for Agilent 1290 Infinity LC Ensure optimum performance by installing the modules of the Agilent 1290 Infinity Binary LC System in the following configuration See Figure 7 on p
14. SER MAC REV AC MFG 2140 0820 848728 no info v information on each module S 10 08 Figure 67 206 LAN Setting on Instant Pilot Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or the main board of above mentioned modules Table 27 G1369C LAN Interface Card configuration switch on the card SW 4 SW5 SW 6 SW7 SW 8 Initialization Mode ON OFF OFF OFF OFF DHCP Table 28 LC Modules inclusive 1120 1220 configuration switch at rear of the instru ment SW6 SW7 SW 8 Initialization Mode ON OFF OFF DHCP 3 Turn on the module that hosts the LAN interface 4 Configure your Control Software e g Agilent ChemStation Lab Advisor Firmware Update Tool and use MAC address as host name e g 0030d3177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 205 Agilent 1200 Infinity Series VWD User Manual 207 13 LAN Configuration Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or
15. SSSSS real serial number Serial Number Information 1260 Infinity The serial number information on the instrument labels provide the following information CCXZZ00000 Format CC Country of manufacturing DE Germany JP Japan e CN China X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module 00000 Serial number Agilent 1200 Infinity Series VWD User Manual 181 12 Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 18 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN optional on board RS 232 Analog APG Remote Special Pumps G1310B Iso Pump 2 G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump Yes No G4220A B Bin Pump 2 No G4204A Quat Pump Yes G1361A Prep Pump 2 Yes No Samplers G1329B ALS 2 G2260A Prep ALS Yes No G1364B FC PS 2 G1364C FC AS G1364D FC uS G1367E HiP ALS K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler Yes No G4226A ALS 2 Yes No Yes 1 Yes Yes No CAN DC OUT for CAN slaves Yes Yes No Yes CAN DC OUT for CAN slaves Yes No THERMOSTAT for
16. Therefore the parameters are lost with the next power cycle of the module Bootp Server Figure 62 Bootp Principle Agilent 1200 Infinity Series VWD User Manual 201 13 LAN Configuration 202 Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the module Thus after a power cycle they are still available This enables a kind of bootp once configuration of the module Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server temporarily powers on the module using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the module Then he selects the initialization mode Using Stored and powers on the module again From now on he is able to establish the TCP IP connection to the module with the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter Figure 63 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when the module shall obtain its parameters from a Bootp Server ever
17. Wavelength Verification gt 3 Calibrate Detector amp Wavelength Calibration UY Lamp On Time 2 86 h Time to Wait Before Wavelength Calibration Omn Wavelength Gap of previous O order Calibra 0 100 nm Wavelength Gap of previous alpha line Calib 0 000 nm Wavelength Gap of 0 order Calibration 0 100 nm Wavelength Gap of alpha line Calibration 0 200 nm Do you want to calibrate the detector using the wavelength verification results by Test Name Wavelength Calibration Module G1314E DE81960002 Status Passed Start Time 6 26 2010 12 53 08 PM Stop Time 5 26 2010 12 56 39 PM CEA EA EN CEN CE E TTT Ly r Test Procedure A 1 Check Prerequisites Y 2 Wavelength Verification Y 3 Calibrate Detector Agilent 1200 Infinity Series VWD User Manual Description This procedure performs a Wavelength Verification and Recalibration Result Name Value Accumulated UY Lamp Burn Time 1389 70 h UY Lamp On Time 2 86 h Time to Wait Before Wavelength Calibration 0 00 min Wavelength Gap of previous O order Calibra 0 100 nm Wavelength Gap of previous alpha line Calib 0 000 nm Wavelength Gap of 0 order Calibration 0 100 nm Wavelength Gap of alpha line Calibration 0 200 nm Figure 41 Wavelength Verification Calibration with Agilent Lab Advisor 121 8 Test Functions ASTM Drift and Noise Test ASTM Drift and Noise Test The ASTM Drift and Noise test determines the detector
18. configuration 197 initialization mode selection 201 link configuration selection 208 manual configuration with telnet 220 manual configuration 219 PC and User Interface Software Setup 225 TCP IP parameter configuration 199 using default 203 using stored 203 what you have to do first 198 eak sensor open 101 eak sensor short 101 eak correcting 145 parts 161 ine frequency 23 ine voltage 23 inearity 24 27 30 ink configuration selection 208 ost CAN partner 100 M MAC address determine 214 maintenance correcting leaks 145 definition of 130 exchanging flow cell 138 exchanging lamps 135 exchanging leak handling system 146 240 for parts see parts for maintenance 149 introduction 129 overview 133 replacing firmware 147 147 standard flow cell 140 using the cuvette holder 143 manual configuration of LAN 219 message ADC hardware error 110 calibration failed 106 calibration lost 109 filter check failed 109 grating filter motor defective 108 heater current missing 106 heater failed 111 heater power at limit 112 holmium oxide test failed 107 ignition without cover 103 103 illegal temperature value from sensor atair inlet 111 illegal value from sensor on main board 110 amp current missing 104 amp ignition failed 105 amp voltage missing 105 no run data available in device 112 remote timeout 99 wavelength check failed 109 method load 64 mirror assemblies 15 Noise and Linearity specif
19. 2 pL on page 154 Semi micro Flow Cell 6 mm 5 pL on page 156 e High Pressure Flow Cell 10 mm 14 uL on page 158 The shown cell parts will differ depending upon the flow cell type For detailed parts schematics refer to above mentioned pages 140 Agilent 1200 Infinity Series VWD User Manual Maintenance and Repair 9 1 Cell screw 2 Conical springs 3 Ring 1 PEEK Gasket 1 small hole Window Quartz Gasket 2 large hole Ring 2 PEEK RFID tag o nN oof Figure 46 Standard Flow Cell 1 Disassembling the Flow Cell a Unscrew the cell screw using a 4 mm hexagonal wrench b Remove the SST rings using a pair of tweezers CAUTION Scratched window surfaces by tweezers Window surfaces can easily be scratched by using tweezers for removing the windows gt Do not use tweezers to remove windows c Use adhesive tape to remove the peek ring the window and the gasket d Repeat step a through step c for the other window keep the parts separate otherwise they could be mixed Agilent 1200 Infinity Series VWD User Manual 141 9 Maintenance and Repair 2 Cleaning the Flow Cell Parts a Pour isopropanol into the cell hole and wipe clean with a piece of lint free cloth b Clean the windows with ethanol or methanol Dry it with a piece of lint free cloth Always use new gaskets 3 Reassembling the Flow Cell a Hold the flow cell cassette horizontally and place gasket in
20. 23x 8 2 Conical springs 3 Ring 2 IN 4 Gasket 2 IN 5 Quartz window 6 Gasket 1 OUT 7 Ring 1 OUT 2 3x 8 RFID Clip 00 1 9 Screw Figure 50 Standard Flow Cell Agilent 1200 Infinity Series VWD User Manual 153 10 Parts and Materials for Maintenance Micro Flow Cell 3 mm 2 pL Item p n G1314 60187 5021 1823 1 79883 22402 5062 8553 3 79883 28801 4 79883 22301 5 1000 0488 6 G1315 68710 7 79883 68702 8 G1314 44010 9 0515 4780 G1314 87301 G1314 87302 G1315 68713 79883 68703 154 Description Micro flow cell 3 mm 2 uL 120 bar with RFID tag Capillary column detector SST 400 mm lg 0 12 mm i d Window screw Washer kit 10 pk Compression washer Window holder Quartz window Gasket FRONT PTFE 1 3 mm hole inlet side 12 pk Gasket BACK PTFE 1 8 mm hole outlet side 12 pk Clip for RFI ID tag Screw for Clip M2 2 4 5 mm long Capillary IN 0 12 mm 310 mm Ig Capillary OUT 0 17 mm 120 mm lg Cell repair kit semi micro includes window screw kit Gasket Kit BACK Gasket Kit FRONT and 4 mm hexagonal wrench Window screw kit includes 2 quartz windows 2 compression washers 2 window holders 2 window screws and 10 washers Agilent 1200 Infinity Series VWD User Manual Parts and Materials for Maintenance 10 Micro Flow Cell 3 mm 2 pL 1 1 Window screw a 2 5x 9 Washers 06 3 8 N 3 Compression washer ES Window holder o Quartz window
21. Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1200 Infinity Series VWD User Manual 169 11 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo o0 S iE Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 170 Agilent 1200 Infinity Series VWD User Manual BCD Cables Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 ee Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital groun
22. IP settings of the PC Agilent 1200 Infinity Series VWD User Manual 225 13 LAN Configuration User Interface Software Setup Install you user interface software according the provided User Interface Software Setup Guide 226 Agilent 1200 Infinity Series VWD User Manual This chapter provides addition information on safety legal and web Agilent 1200 Infinity Series VWD User Manual 14 Appendix General Safety Information 228 Safety Symbols 228 General Safety Information 229 Safety Standards 229 Operation 229 Radio Interference 231 Sound Emission 232 UV Radiation 233 Solvent Information 234 Declaration of Conformity for HOX2 Filter 236 Agilent Technologies on Internet 237 ee Agilent Technologies 227 14 Appendix General Safety Information Safety Symbols Table 31 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up WARNING aii alerts you to situations that could cause physical injury or death gt Do not proc
23. Infinity Series VWD User Manual Using the Detector 4 Table 12 Peak Width Response Time Data Rate G1314E peakwidth at half heigth Response Time s Data Rate Hz min lt 0 0012 lt 0 03 160 gt 0 0012 0 03 160 gt 0 0025 0 06 160 gt 0 005 0 12 80 gt 0 01 0 25 40 gt 0 025 0 5 20 gt 0 05 1 0 10 gt 0 1 2 0 5 gt 0 2 4 0 2 5 gt 0 4 8 0 1 25 Agilent 1200 Infinity Series VWD User Manual 79 4 Using the Detector Run Recovery G1314E CAUTION This feature is not supported with ChemStation B 03 02 SR1 It will be implemented with ChemStation B 04 01 The figures used in this chapater are from the G1315C DAD VL The G1314E VWD figures will look the same Run Recovery Settings For this recovery mode the CompactFlash Card must be in the detector If the LAN communiaction is interruppted no data is stored gt Have the CompactFlash Card always inserted 80 The detector supports run buffering which means that an amount of run data uv and ch files is stored in a storage medium CompactFlash Card in the detector until either it is overwritten or the detector undergoes a power cycle If there is a temporary network failure or the PC is not able to constantly take the data the stored data is transferred to the ChemStation automatically when the network connection is restored or the PC can take the data so that no loss of data occurs If there is a permanent network fail
24. Leak Parts 162 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 11 Identifying Cables Cable Overview 164 Analog Cables 166 Remote Cables 168 BCD Cables 171 CAN LAN Cables 173 RS 232 Cables 174 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ee Agilent Technologies 163 11 Identifying Cables Cable Overview 164 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 168 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose Agilent 1200 Infinity Series VWD User Manual Identifying Cables 11 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables
25. MAC Label 2 Connect the instrument s LAN interface to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable 198 Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 TCP IP parameter configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask Default Gateway The TCP IP parameters can be configured by the following methods e by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol by automatically requesting the parameters from a network based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 206 e by manually setting the parameters using Telnet e by manually setting the parameters using the Instant Pilot G4208A The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch see Table 25 on page 201 Agilent 1200 Infinity Series VWD User
26. Manual 199 13 LAN Configuration Configuration Switches The configuration switch can be accessed at the rear of the module see figure below Te 5 5 C5 i eae CAN GAR ye 85 UN a HUN e Bele COMPACT FLASH Figure 61 Location of Configuration Switch The module is shipped with all switches set to OFF as shown above To perform any LAN configuration SW1 and SW2 must be set to OFF Table 24 Factory Default Settings Initialization Init Mode Bootp all switches down For details see Figure 62 on page 201 Link Configuration speed and duplex mode determined by auto negotiation for details see Link configuration selection on page 208 200 Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Initialization mode selection Table 25 The following initialization init modes are selectable Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default ON OFF OFF DHCP 1 Requires firmware B 06 40 or above Modules without LAN on board see G1369C LAN Interface Card Bootp When the initialization mode Bootp is selected the module tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the module
27. Parts required 146 If the parts are corroded or broken None p n Description 5041 8389 Leak funnel holder 5041 8388 Leak funnel 5062 2463 Corrugated tubing PP 6 5 mm id 5 m Remove the front cover to have access to the leak handling system Pull the leak funnel out of the leak funnel holder Pull the leak funnel with the tubing out of its location Replace the leak funnel and or the tubing Insert the leak funnel with the tubing in its position Insert the leak funnel into the leak funnel holder Replace the front cover Leak funnel Leak funnel holder Leak tubing Figure 49 Replacing Waste Handling System Parts Agilent 1200 Infinity Series VWD User Manual Maintenance and Repair Replacing the Module s Firmware When Tools required OR OR Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and doc
28. Service using Notepad The default location for the logfile is C Documents and Settings All Users Application Data Agilent BootP LogFile e The logfile will not be updated if it is open The contents will be similar to the following 02 25 10 15 30 49 PM Status BootP Request received at outermost layer Status BootP Request received from hardware address 0010835675AC Error Hardware address not found in BootPTAB 0010835675AC Status BootP Request finished processing at outermost layer 3 Record the hardware MAC address for example 0010835675AC The Error means the MAC address has not been assigned an IP address and the Tab File does not have this entry The MAC address is saved to the Tab File when an IP address is assigned 5 Close the log file before turning on another instrument Uncheck the Do you want to log BootP requests box after configuring instruments to avoid having the logfile use up excessive disk space 214 Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Adding each instrument to the network using BootP 1 Follow Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 Uncheck the Do you want to log BootP requests once all instruments have been added The Do you want to log BootP requests box must be unchecked when you have finished configuring instruments otherwise the log file will quickly fill up disk space
29. The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause Suggested actions 1 The temperature sensor is defect e Replace the cable to the main board e Please contact your Agilent service representative 2 Detector is exposed to illegal ambient Verify that the ambient conditions are within conditions the allowed range Heater at fan assembly failed Error ID 1073 Every time the deuterium lamp or the tungsten lamp DAD only is switched on or off a heater self test is performed If the test fails an error event is created As a result the temperature control is switched off Probable cause Suggested actions 1 Defective connector or cable Please contact your Agilent service representative 2 Defective heater Please contact your Agilent service representative Agilent 1200 Infinity Series VWD User Manual 111 7 Error Information Heater Power At Limit Error ID 1074 The available power of the heater reached either the upper or lower limit This event is sent only once per run The parameter determines which limit has been hit 0 means upper power limit hit excessive ambient temperature drop 1 means lower power limit hit excessive ambient temperature increase Probable cause Suggested actions 1 Excessive ambient temperature change Wait until temperature control equilibrates No Run Data Available In Device In
30. WARNING Toxic flammable and hazardous solvents samples and reagents gt Keep solvent path free from blockages gt Keep the flow path closed in case the pump in the system is equipped with a passive inlet valve solvent may leak out due to hydrostatic pressure even if your instrument is off gt Avoid loops gt Tubes must not sag gt Do not bend tubes gt Do not immerse tube end in waste liquid gt Do not intubate tubes in other tubes gt For correct tubing follow instructions on label attached to the module Agilent 1200 Infinity Series VWD User Manual 47 3 Installing the Detector tallation Informe Warning Attention Warnung Attenzione Advertencia Disconnect power cord before moving D branchez le cordon secteur avant tout d placement Entfernen Sie das Netzkabel bevor Sie das Gerat bewegen Scollegare il cavo di alimentazione prima di spostare lo strumento Desconectar el aparato de la red antes de moverlo L 2J I Figure 14 Warning label illustration for correct waste tubing 48 Agilent 1200 Infinity Series VWD User Manual Installing the Detector Parts required 1 1 1 1 1 1 p n G4208A Installing the Detector 3 Description Detector Power cord LAN cable cross over or twisted pair network cable Agilent ChemStation or other control software Instant Pilot CompactFlash Card G1314E only For other cables see below and section Cable Overvi
31. a Gasket front N Gasket back foe RFID clip 9 Screw Figure 51 Micro Flow Cell Agilent 1200 Infinity Series VWD User Manual 155 10 Parts and Materials for Maintenance Semi micro Flow Cell 6 mm 5 pL The semi micro 1 and 2 gaskets items 6 and 7 look very similar Do not mix them up Item p n G1314 60183 5021 1823 1 G1314 20047 G1314 65056 2 79853 29100 3 79853 22500 4 79853 68743 5 79853 68742 6 7 8 G1314 44010 9 0515 4780 156 Description Semi micro flow cell 6 mm 5 pL with RFID tag Capillary column detector SST 400 mm lg 0 12 mm i d Cell screw Semi micro cell kit includes two quartz windows one gasket 1 one 2 and two PTFE gaskets Conical spring kit 10 pk Ring SST 2 pk PTFE gasket round hole i d 2 5 mm o d 8 mm 10 pk Window quartz kit 2 pk Semi micro 1 gasket long hole 1 5 x 3 5 mm PTFE Semi micro 2 gasket long hole 2 x 4 mm PTFE Clip for RFI ID tag Screw for Clip M2 2 4 5 mm long Agilent 1200 Infinity Series VWD User Manual Cell screw 2 Conical springs 3 Ring SST 4 PTFE gasket 5 Quartz window 6 Semi micro gasket 1 7 Semi micro gasket 2 8 Clip for RFI ID tag 9 Screw for clip Figure 52 Semi micro Flow Cell Agilent 1200 Infinity Series VWD User Manual Parts and Materials for Maintenance Semi micro Flow Cell 6 mm 5 pL 10 157 10 Parts and Materials for Maintenance H
32. cells have RFID tags for unambitious and 40 bar 588 psi pressure maximum identification High pressure 14 uL volume cell path length Can be repaired on component level and 400 bar 5880 psi pressure maximum Micro 2 uL volume 3 mm cell path length and 120 bar 1760 psi pressure maximum Semi micro 5 pL volume 6 mm cell path length and 40 bar 588 psi pressure maximum 24 Agilent 1200 Infinity Series VWD User Manual Site Requirements and Specifications 2 Table3 Performance Specifications Type Specification Comments Electronic For improved baseline stability in instable Temperature Control environment ETC Control and data Agilent ChemStation B 03 02 SR1 or above Control and data evaluation evaluation Instant Pilot G4208A with firmware B 02 07 or Control only above Time programmable Wavelength Reference and Sample scan balance steps lamp on off Spectral tools Stop flow wavelength scan Analog outputs Recorder integrator 100 mV or 1 V output range 0 001 2 AU one output Communications LAN card integrated on main board Controller area network CAN RS 232C APG Remote ready start stop and shut down signals Safety and Extensive diagnostics error detection and maintenance display through Instant Pilot and Data System leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas GLP features Early maintenance feedback EMF for
33. continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages Electronic records of maintenance and errors Verification of wavelength accuracy with built in holmium oxide filter RFID for electronics records of flow cell and UV lamp conditions path length volume product number serial number test passed usage Housing All materials recyclable Agilent 1200 Infinity Series VWD User Manual 25 2 Site Requirements and Specifications Specification Conditions G1314D ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions Standard flow cell path length 10 mm flow 1 mL min LC grade methanol Noise 0 15 10 AU at 230 nm TC 2 s RT 2 2 TC Linearity Linearity is measured with caffeine at 265 nm The specification are based on the the standard RFID tag lamp G1314 60101 and may be not achieved when other lamp types or aged lamps are used ASTM drift tests require a temperature change below 2 C hour 3 6 F hour over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Tur
34. from 6 mm to 10 mm When increasing the path length the cell volume usually increases in the example from 5 14 uL Typically this causes more peak dispersion As demonstrated this did not affect the resolution in the gradient separation in the example that is shown below As a rule of thumb the flow cell volume should be about 1 3 of the peak volume at half height To determine the volume of your peaks take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3 Agilent 1200 Infinity Series VWD User Manual How to optimize the detector 5 Analysis of pesticide standard 6 mm optical path length Absorbance T0 mm optical pathlength T i j j i A f I A f h j A id N DO Ua ee WV 2 l a een cee ie oat 1g 20 38 Time min Figure 36 Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards To check photometric accuracy of the detector it is necessary to have more precise information on path lengths of the flow cells The correct response is expected response correction factor Please find below the details of the flow cells Table 14 Correction factors for Agilent VWD flow cells Part number Path length actual Correction factor Standard flow cell 10 mm 14 uL 40 bar G
35. gt one hour ASTM measurements require that the detector should be turned on at least 24 hours before start of testing Agilent 1200 Infinity Series VWD User Manual 29 2 Site Requirements and Specifications Performance Specifications G1314F Table 5 Performance Specifications G1314F Type Specification Comments Detection type Light source Wavelength range Short term noise Drift Linearity Wavelength accuracy Maximum data rate Band width Flow cells Electronic Temperature Control ETC Double beam photometer Deuterium lamp 190 600 nm 0 25 10 AU at 230 nm lt 1 104 AU h at 230 nm gt 2 5 AU 5 at 265 nm 1nm 80 Hz 6 5 nm typical Standard 14 pL volume 10 mm cell path length and 40 bar 588 psi pressure maximum High pressure 14 uL volume cell path length and 400 bar 5880 psi pressure maximum Micro 2 uL volume 3 mm cell path length and 120 bar 1760 psi pressure maximum Semi micro 5 pL volume 6 mm cell path length and 40 bar 588 psi pressure maximum For improved baseline stability in instable environment The UV lamp is equipped with RFID tag that holds lamp typical information Under specified condtions See Specification Conditions G1314F on page 32 below the table Under specified condtions See Specification Conditions G1314F on page 32 below the table Under specified condtions See Specification Conditions G1314
36. is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Agilent 1200 Infinity Series VWD User Manual 21 2 Site Requirements and Specifications Bench Space The detector dimensions and weight see Physical Specifications on page 23 allows you to place the detector on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 8 1 inch in the rear for air circulation and electric connections If the bench
37. lamp is connected Exchange the lamp Please contact your Agilent service representative Please contact your Agilent service representative 105 7 106 Error Information No heater current Error ID 7453 The lamp heater current in the detector is missing During lamp ignition the processor monitors the heater current If the current does not rise above the lower limit within 1 the error message is generated Probable cause 1 Lamp disconnected 2 Ignition started without the top foam in place 3 Defective main board 4 Defective or non Agilent lamp 5 Defective power supply Wavelength calibration setting failed Error ID 7310 Suggested actions Ensure the lamp is connected Please contact your Agilent service representative Please contact your Agilent service representative Exchange the lamp Please contact your Agilent service representative The intensity maximum was not found during wavelength calibration Calibration 0 Failed Calibration 1 Failed Probable cause 1 Lamp is OFF 2 Incorrect flow cell installation 3 Flow cell contamination or air bubbles Zero order calibration failed 656 nm calibration failed Suggested actions Switch on the lamp Ensure the flow cell is installed correctly Clean replace flow cell windows or remove air bubbles Agilent 1200 Infinity Series VWD User Manual Probable cause 4 Intensity too low 5 Current step value
38. noise over a period of 20 minutes The test is done with HPLC grade water flowing through the flow cell at 1 mL min On completion of the test the noise result is displayed automatically Test Name ASTM Drift and Noise Test Description The test performs ASTM Drift and Noise evaluation without Module G1314E DE81980002 reference Status Passed Start Time 5 26 2010 11 59 46 AM Stop Time 5 26 2010 12 19 46 PM m Test Procedure Result W 1 Check Prerequisites Accumulated UY Lamp Bum Time 1388 81 h WY 2 Measure Noise UVY Lamp On Time 1 97 h ZA 3 Evaluate Data Signal Drift value at 254 nm UV 0 062 m amp U h Signal Noise value at 254 nm UV 0 004 m U Absorbance mAU 22 059 22 07 22 08 22 09 22 1 22 107 o 4 6 10 Time min Figure 42 ASTM Drift and Noise Test with Agilent Lab Advisor 122 Agilent 1200 Infinity Series VWD User Manual Test Functions 8 Quick Noise Test Quick Noise Test The noise test measures the noise of the detector with HPLC grade water flowing through the flow cell at 1 mL min in one minute intervals over a total of 5 minutes The noise of the detector is calculated by using the maximum amplitude for all random variations of the detector signal of frequencies greater than one cycle per hour The noise is determined for 5 one minute intervals and is based on the accumulated peak to peak noise for the intervals At least seven data poi
39. parameters and the flow cell 6 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features Agilent 1200 Infinity Series VWD User Manual 3 In This Guide 7 Error Information This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from error conditions 8 Test Functions This chapter describes the detector s built in test functions 9 Maintenance and Repair This chapter provides general information on maintenance and repair of the detector 10 Parts and Materials for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 12 Hardware Information This chapter describes the detector in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connecting the detector to the Agilent ChemStation PC 14 Appendix This chapter provides addition information on safety legal and web Agilent 1200 Infinity Series VWD User Manual Contents Contents Introduction to the Variable Wavelength Detector 9 Introduction to the Detector 10 Optical System Overview 11 System Overview 16 Site Requirements and Specifications 19 Site Requirements 20 Physical Specifications 23 Performance Specifications G1314D 24 Perfo
40. should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Agilent 1200 Infinity Series VWD User Manual 185 12 Hardware Information 186 Table 19 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 n 2 RX TX 3 X 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 M 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 58 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 APG Remote The APG Remote connector may be used in combination with other analytica
41. switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface Agilent 1200 Infinity Series VWD User Manual 97 7 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message 98 Agilent 1200 Inf
42. the detector Optimizing the Detector Performance The detector has a variety of parameters that can be used to optimize performance The information below will guide you on how to get the best detector performance Follow these rules as a start for new applications It gives a rule of thumb for optimizing the detector parameters 84 Agilent 1200 Infinity Series VWD User Manual How to optimize the detector 5 Match the Flow Cell to the Column The tables below recommend the flow cell that matches the column used If more than one selection is appropriate use the larger flow cell to get the best detection limit Use the smaller flow cell for best peak resolution Standard HPLC Applications Column length Typical peak width Recommended flow cell lt 5 cm 0 025 min Micro flow cell 10 cm Semimicro flow cell 7 cm E 1min gt ETTE mn ie ypical flow rate n 05 0 2 ml min 2 0 4 ml min EER 4 0 8 ml min ene 01 5 ml min Internal column diameter 10mm 21mm 3 0 mm 46mm Figure 34 Choosing a Flow Cell Standard HPLC Applications Ultra fast separation with RRLC systems Column ID 2 1mm 3 0 mm 46mm Configuration No damper Damper Damper No mixer Mixer Mixer Flow cell 2 ul 3mm 5 ul 6mm 14 ul 10 mm Figure 35 Choosing a Flow Cell for G1314E for ultra fast separation with RRLC systems For ultra fast analysis with s
43. too far from maximum 6 Misaligned defective grating assembly 7 Defective main board Wavelength holmium check failed Error ID 7318 Error Information 7 Suggested actions Replace lamp e Repeat the calibration e Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The holmium oxide test in the detector has failed During the holmium test the detector moves the holmium filter into the light path and compares the measured absorbance maxima of the holmium oxide filter with expected maxima If the measured maxima are outside the limits the error message is generated Probable cause 1 Misaligned defective grating assembly Agilent 1200 Infinity Series VWD User Manual Suggested actions Ensure the flow cell is inserted correctly and is free from contamination cell windows buffers and so on Run the filter motor test to determine if the filter motor assembly is defective If defective please contact your Agilent service representative Run the grating motor test to determine if the grating assembly is defective If defective please contact your Agilent service representative 107 7 Error Information Grating or Filter Motor Errors Error ID Grating 7800 7801 7802 7803 7804 7805 7806 7808 7809 Filter 7810 7811 7812 7813 7814 7815 7816 The motor test has failed
44. touch one of the metal housing panels at the front of the instrument Agilent 1200 Infinity Series VWD User Manual 135 Maintenance and Repair Exchanging a Lamp Agilent 1200 Infinity Series VWD User Manual 2 Unscrew the heater assembly and remove it have access to the front area reconnect the lamp 3 Unscrew disconnect and remove the lamp Insert fixand 4 Replace the heater assembly 1 Press the release buttons and remove the front cover to 136 Maintenance and Repair 9 Next Steps Replace the front cover Reset the lamp counter as described in the User Interface documentation required for non RFID tag lamps only Turn the lamp ON Give the lamp more than 10 minutes to warm up oo N O amp O ol Perform Wavelength Verification Calibration on page 120 to check the correct positioning of the lamp If the detector was turned off during the replacement then the detector requires a warm up time of 60 minutes No measurements should be performed during this time Agilent 1200 Infinity Series VWD User Manual 137 9 Maintenance and Repair Exchanging a Flow Cell When If application needs a different type of flow cell or the flow cell needs repair Tools required Description Wrench 1 4 inch for capillary connections Parts required Description 1 Flow cell For flow cell details see e Standard Flow Cell 10 mm 14 uL on page 152
45. 02 Status Passed Start Time 5 26 2010 12 50 42 PM Stop Time 5 26 2010 12 51 10 PM m Test Procedure Result A 1 Check Prerequisites Maximum Sample Intensity Measured 37 Counts 7A 2 Perform Dark Current Test Maximum Reference Intensity Measured 34 Counts A 3 Evaluate Data Intensity Counts 37 20 1 500 Wavelength nm Intensity Counts 34 20 1 500 wavelength nm Figure 44 Dark Current Test with Agilent Lab Advisor 124 Agilent 1200 Infinity Series VWD User Manual Test Functions 8 Dark Current Test Failed Probable cause Suggested actions 1 Defective sample or reference diode Please contact your Agilent service representative 2 Defective sample or reference ADC board Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1200 Infinity Series VWD User Manual 125 Test Functions Holmium Oxide Test 126 This test verifies the calibration of the detector against the three wavelength maxima of the built in holmium oxide filter The test displays the difference between the expected and measured maxima The figure below shows a holmium test spectrum The Holmium Oxide Test is available in Agilent Lab Advisor preferred tool e Agilent Instant Pilot G4208A via More Diagnosis VWD Holmium Spectrum Test The test uses the following holmium maxima e 360 8 nm e 418 5 nm 536 4 nm
46. 0A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 184 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface
47. 1 Index available tests vs interface 93 diagnostic signals 90 error messages 90 97 overview 90 status indicators 90 91 test functions 115 90 U unpacking 34 using analog output settings 75 control settings 71 detector parameters 65 drift 63 EMF 194 load method 64 online plot 68 online spectra 73 peakwidth settings 76 preparing the HPLC system 61 priming and purging the system requirements and conditions 59 running the sample 70 sample info 69 scanning 74 setting up an analysis 57 setup of detector 65 signal plot 67 special setpoints 76 special settings 71 stable baseline 63 stop flow condition 73 the cuvette holder 143 the detector 55 turnon 62 typical chromatogram 60 warmup 63 UV radiation 233 242 57 V voltage range 23 wW warm up of detector 63 warnings and cautions 131 wavelength accuracy 30 27 24 calibration 120 range 190 600 nm 30 27 24 recalibration 90 weight 23 Agilent 1200 Infinity Series VWD User Manual Index Agilent 1200 Infinity Series VWD User Manual 243 www agilent com In This Book This manual contains technical reference information about the Agilent 1290 Infinity Variable Wavelength Detector G1314E the Agilent 1260 Infinity Variable Wavelength Detector G1314F and the Agilent 1200 series Variable Wavelength Detector G1314D obsolete The manual describes the following e introduction and specifications e installation using an
48. 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the module is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the module Table 29 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF manually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full duplex 208 Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Automatic configuration with Bootp All examples shown in this chapter will not work in your environment You need your own IP Subnet Mask and Gateway addresses Assure that the detector configuration switch is set properly The setting should be either BootP or BootP amp Store see Table 25 on page 201 Assure that the detector connected to the network is powered off If the Agilent BootP Service program is not already installed on your PC then install it from your Agilent ChemStation DVD located in folder BootP About Agilent BootP Service The Agilent BootP Service is used to assign the LAN Interface with an IP address The Agilent BootP Service is provided on the ChemStat
49. 11 Identifying Cables Remote Cables 168 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Agilent 1200 Infinity Series VWD User Manual Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground ae NC 2 Brown Prepare run Low o i 3 3 Gray Start Low z NC 4 Blue Shut down Low a NC 5 Pink Not Ls connected Se NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D
50. 1314 60186 10 15 0 19 mm 10 10 15 Semi micro flow cell 6 mm 5 pL G1314 60183 6 10 0 19 mm 6 6 10 Micro flow cell 3 mm 2 uL 120 bar G1314 60187 2 80 0 19 mm 3 2 8 High pressure flow cell 10 mm 14 pL 400 bar G1314 60182 10 00 0 19 mm 10 10 However you have to be aware that there is additional tolerance of gasket thickness and its compression ratio which is supposed to be very small in comparison with the machining tolerance Agilent 1200 Infinity Series VWD User Manual 87 5 How to optimize the detector Set the Detector Parameters 1 Set peakwidth as close as possible to the width at half height of a narrow peak of interest 2 Choose the sample wavelength at a longer wavelength than the cut off wavelength of the mobile phase at a wavelength where the analytes have strong absorptivity if you want to get the lowest possible detection limit at a wavelength with moderate absorptivity if you work with high concentrations and preferably where the spectrum is flat for better linearity 3 Consider to use time programming to further optimization 88 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 6 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 90 Status Indicators 91 Power Supply Indicator 91 Module Status Indicator 92 Available Tests versus Interfaces 93 Agilent Lab Advisor Software 94 Overview about the t
51. 3 Dark Current Test 124 Dark Current Test Failed 125 Holmium Oxide Test 126 Holmium Oxide Test Failed 127 Ee Agilent Technologies 115 Test Functions Intensity Test 116 The intensity test measures the intensity of the deuterium lamp over the full VWD wavelength range 190 600 nm The test can be used to determine the performance of the lamp and to check for dirty or contaminated flow cell windows When the test is started the gain is set to zero To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The shape of the intensity spectrum is primarily dependent on the lamp grating and diode characteristics Therefore intensity spectra will differ slightly between instruments The figure below shows a typical intensity test spectrum The Intensity Test is available in Agilent Lab Advisor preferred tool e Agilent Instant Pilot G4208A via More Diagnosis VWD Lamp Intensity Test Intensity Test Evaluation The Agilent Lab Advisor and the Instant Pilot evaluate three values automatically and display the limits for each value the average the minimum and the maximum of all data points and passed or failed for each value Agilent 1200 Infinity Series VWD User Manual Test Functions 8 Intensity Test Instensity Test with Agilent Lab Advisor Test Name Intensity Test Description The test scans the Intensity spectrum generated by the UV Module G1314E DE81360002 Eai Stat
52. 77 uL Materials in contact with solvent SST quartz PTFE PEEK SST quartz PTFE SST quartz PTFE SST quartz Kapton 12 Agilent 1200 Infinity Series VWD User Manual Introduction to the Variable Wavelength Detector 1 Lamp The light source for the UV wavelength range is a deuterium lamp As a result of plasma discharge in a low pressure deuterium gas the lamp emits light over the 190 600 nm wavelength range The lamp has an integrated RFID tag that contains the lamp specific information e g part number burn time A RFID tag reader reads out this information and transfers it to the user interface Source Lens Assembly The source lens receives the light from the deuterium lamp and focuses it onto the entrance slit Entrance Slit Assembly The entrance slit assembly has an exchangeable slit The standard one has a l mm slit For replacement and calibration purposes to optimize the alignment a slit with a hole is needed Agilent 1200 Infinity Series VWD User Manual 13 1 Introduction to the Variable Wavelength Detector Filter Assembly The filter assembly is electromechanically actuated During wavelength calibrations it moves into the light path Cutoff Filter plus Holmium Oxide Filter Shutter Cutoff Filter Figure 3 Filter Assemby The filter assembly has two filters installed and is processor controlled OPEN nothing in light path at A lt 370 nm CUTOFF cu
53. Cancel E c E chem32 amp 2 BATCH M M DEMOCAL2 M DGALSTST M E gt methods DGCALAS M s DGCALOG1 M DGCALOQ2 M Drives c SYSTEM z Network Fie RunControl Instrument Method Sequence View Abort Help amp Methods LA dy DEF Lem x sequences Ge bed ver ics Fis BA BAG of Method and Run Control EHEJ C CHEM32 2 METHODS T e E 0 etic oer tc R secuence oers E BATCH M Senp Sino QI DEMOCALL M QI DEMOCAL2 M 1 QI DGALSTST M QI DGCALAS M VWD DGCALOQ1 M wu fo 1 QI DecaLog2 m I DGCALOQ3 M QI DGCALOQ4 M QI DecaLogs m QI DECALOge M DEFAULT D QI DGCALPS M QI DGCHPTST M AI DGCPTEST M QI DGFLDTST M QI DGLEAKT M QI DGNOISE M QI DGNPTEST M AI DGTHMTST M QI DGYWDDC M E data Q Isocra m E 1 1 GB free Q ISOPLOT M a Figure 20 Loading Default LC Method no Details 7 64 Agilent 1200 Infinity Series VWD User Manual Method and Run Control Using the Detector 4 ng up an Analysis 9 Click on the module icons Figure 21 on page 65 and open the Setup of these modules Figure 22 on page 66 shows the detector settings do not change the detector parameters at this time DEFAULT D E Adata E 1 1 GB free of Control fu Online Spectra B Not Ready Information Help Figure 21 Open the module menu Agilent 1200
54. Conditions G1314E on page 29 below the table Under specified condtions See Specification Conditions G1314E on page 29 below the table Self calibration with deuterium lines verification with holmium oxide filter All flow cells have RFID tags for unambitious identification Can be repaired on component level Agilent 1200 Infinity Series VWD User Manual 27 2 Site Requirements and Specifications Table4 Performance Specifications G1314E Type Specification Comments Control and data Agilent ChemStation B 03 02 SR1 or Control and data evaluation evaluation above Control only Instant Pilot G4208A with firmware B 02 07 or above Time programmable Wavelength Reference and Sample scan balance steps lamp on off Spectral tools Stop flow wavelength scan Analog outputs Recorder integrator 100 mV or 1 V output range 0 001 2 AU one output Communications LAN card integrated on main board Controller area network CAN RS 232C APG Remote ready start stop and shut down signals Safety and Extensive diagnostics error detection maintenance and display through Instant Pilot and Data System leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas GLP features Early maintenance feedback EMF for continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages Electronic r
55. D Tag is available Use UV lamp anyway Lamp Type Cell Tag use Cell only if RFID Tag is available use Cell anyway Cancel Help Temperature Control the optical unit is kept on constant temperature some degrees above ambient and improves the baseline stability in unstable environments See also note below UV lamp tag Automatic mode for Agilent lamps with RFIDtags If no RFID tag lamp is used detector icon will become gray lamp tag not ready and analysis is disabled The specifications are based on the lamp with RFID tag Use UV lamp anyway here you can select non RFID tag lamps such as of type VWD lor DAD have different heating mechanismn The correct selection is important for optimal performance and lifetime Cell tag for Agilent flow cells with RFID tags If no RFID tag cell is used detector icon will become gray cell tag not ready and analysis is disabled Help online help The detector status shows Cell tag in yellow in case the flow cell with the RFID tag is not inserted The detector icon is gray and the system is not ready WWD Status MPEG cetag Figure 28 Detector Configuration Settings If the flow cell temperature is critical for your chromatography or your environment is stable you may set the Temperature Control to off This will lower the optical unit and flow cell temperature by some degree 72 Agilent 1200 Infinity Series VWD User Manual
56. F on page 32 below the table Self calibration with deuterium lines verification with holmium oxide filter All flow cells have RFID tags for unambitious identification Can be repaired on component level 30 Agilent 1200 Infinity Series VWD User Manual Site Requirements and Specifications 2 Table5 Performance Specifications G1314F Type Specification Comments Control and data Agilent ChemStation B 04 02 SP2 or Control and data evaluation evaluation above Control only Instant Pilot G4208A with firmware B 02 11 or above Time programmable Wavelength Reference and Sample scan balance steps lamp on off Spectral tools Stop flow wavelength scan Analog outputs Recorder integrator 100 mV or 1 V output range 0 001 2 AU one output Communications LAN card integrated on main board Controller area network CAN RS 232C APG Remote ready start stop and shut down signals Safety and Extensive support for troubleshooting maintenance and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Safety related features are leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas GLP features Early maintenance feedback EMF for continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages Electronic records of maintenance and err
57. ING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 134 Agilent 1200 Infinity Series VWD User Manual Maintenance and Repair 9 Exchanging a Lamp When Tools required Parts required Preparations If noise or drift exceeds application limits or lamp does not ignite Description Screwdriver Pozidriv 1 PT3 p n Description 1 G1314 60101 Deuterium lamp with RFID tag Turn the lamp OFF If you want to use an Agilent DAD lamp instead of the VWD lamp you have to change the lamp settings in the VWD Configuration to the required lamp type This ensures that the DAD lamp s filament heating is operated like in the DAD The specification are based on the the standard RFID tag lamp G1314 60101 and may be not achieved when other lamp types or aged lamps are used Injury by touching hot lamp If the detector has been in use the lamp may be hot gt If so wait for lamp to cool down Injury by sharp metal edges gt Be careful when touching the RFI sheet metal at the rear of the fan There are sharp edges CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD gt To prevent accidental electrostatic discharge when coming into contact with components inside the instrument
58. Infinity Series VWD User Manual 65 4 Using the Detector Setting up an Analysis 10 Enter the pump parameters mentioned under Table 9 on page 59 i I 2 Soph ccd aya 5 D Signari ni 1 signal with individual wavelength setting stop and post time can be set if required J254 time table for programmable actions during the run botmin 2a Z Zero Offset Limits 1 to 99 in steps of 1 Attenuation Limits 0 98 to 4000 mAU at discrete values for either 100 mV or 1 V full scale additional signals can be stored with the normal signal for diagnostics autobalance to zero absorbance on the analog output plus offset at begin and or end of run see Special Setpoints on page 76 Figure 22 Detector Settings default 66 Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 11 Pump the water acetonitrile 80 70 mobile phase through the column for 10 minutes for equilibration 12 Click the button and select Change to open the Signal Plot information Select the Pump Pressure and the VWD A Signal 254 as signals Change the Y range for the VWD to 1 mAU and the offset to 20 and the pressure offset to 50 The X axis range should be 15 minutes Press OK to exit this screen Method and Run Control Resa e OI eos oer con ag ec oer ics EME IMO A 2 i PE omg Why TEO TA B C D perro ede Vso E no Details 7 7 Edit Signal Plot ae A
59. Intensity Ratio 057 Figure 39 Cell Test with Lab Advisor Checking the Photocurrent with the Instant Pilot Diagnosis Intensity VWD SL At 254 nm sample cts measured 4018723 Done At 254 nm reference cts measured 3612108 Done Max intens gt 320 000 cts measured 6039917 Passed Min intens gt 6 400 cts measured 25507 Passed Avrg intens gt 160 000 cts measured 884724 Passed Finished Passed Y G Exit Figure 40 Checking the Photocurrent with the Instant Pilot Agilent 1200 Infinity Series VWD User Manual 119 Test Functions Wavelength Verification Calibration 120 Wavelength calibration of the detector is done using the zero order position and 656 nm emission line position of the deuterium lamp The calibration procedure involves two steps First the grating is calibrated on the zero order position The stepper motor step position where the zero order maximum is detected is stored in the detector Next the grating is calibrated against the deuterium emission line at 656 nm and the motor position at which the maximum occurs is stored in the detector In addition to the zero order and 656 nm alpha emission line calibration the beta emission line at 486 nm and the three holmium lines are used for the complete wavelength calibration process These holmium lines are at 360 8 nm 418 5 nm and 536 4 nm The wavelength verification calibration take
60. Limits No real limit The reason is that it depends on the position alignment of the reference side beam splitter reference slit reference diode Therefore the reference side value can be higher smaller than the sample side value With a clean cell the counts for sample and reference photocurrent are in the same range If the sample side shows much lower values than the reference side the flow cell might have a problem Pre requisite Flush the flow cell with a flow of 1 mL min for at least 10 minutes Probable Cause Suggested Action Cell contaminated Flush flow cell Cell windows are contaminated Clean replace cell windows Mechanical problem Check cell position In the Agilent Instant Pilot G4208A the photocurrent readings are available via More gt Diagnosis gt VWD gt Lamplntensity Test see Figure 40 on page 119 Agilent 1200 Infinity Series VWD User Manual Test Functions Cell Test Test Name Cell Test Description Calculate the ratio of the sample signal and the reference signal measured in the zero order of the grating Module G1314C DE60555128 Status Passed Start Time 7 6 2011 1 24 55 PM Stop Time 7 6 2011 1 26 18 PM a Test Procedure Result B Name Value WY 1 Check Prerequisites Accumulated UV Lamp Burn Time 60 49h 7 2 Flush Fow Cel UV Lamp On Time 436h 7 3 Measure Sample and Reference Intensity Intensity Sample 241 908 Counts y A Eia Intensity Reference 422 625 Counts aa a
61. PC and User Interface Software Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see also Initialization mode selection on page 201 FIXED IP address Local Area Connection Properties 2 x Internet Protocol General General Connect using You can get IP setings assigned automatically if your network supports this capability Otiierwise you need to ask your network administrator for the appropriate IPiisettings a Xircom CreditCard Ethemet 10 100 Modem 56 _Lertgse C_Dbierrarrtatiireseautomatically Use the following IP address IP adiere 192 168 254 1 Subnet mask 255 255 248 0 Default gateway p Cc Install Uninstall Properties Ji ER Use the folo General Transmission Control Protocol Intemet Protocol The default pEenered ONS You can get IP settings assigned automatically if your network supports ie ne cali Dre is De ies connie Altemate DNS this capability Otherwise you need to ask your network administrator for the appropriate IP settings Show icon in taskbar when connected Obtain an IP address automatically C Demeter scares automatic IP address via DHCP tli I requires special IT setup of the network Obtain DNS server address automatically C Use the following DNS server addresses Figure 81 Changing the TCP
62. Serial s 3 DGNOT Columns i INSTPE a LOADT Configure 1100 1200 Access 5 ml min 4 When the peak of interest enters the flow cell stop the flow set flow rate to zero or open the purge valve and wait a few moments to stabilize the concentration Turning off the pump would stop the run and no access to the sample scan is possible 5 Select from the menu Instrument gt More VWD gt Sample Scan A sample scan is taken in the range defined under Special Setpoints on page 76 and the Online Spectra window see Online Spectra on page 73 displays the result Sample Scan minus Blank Scan 74 Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 Special Settings of the Detector Analog Output Settings 1 To change the Output Range of the analog outputs select VWD Control 2 To change the offset and the attenuation select VWD Signal gt More WD Control System 2 Analog Output Range can be set to either 100 mV or 1 V full scale Zero Offset can be set to either 100 mV or 1 V full scale Attenuation Limits 0 98 to 4000 mAU at discrete values for either 100 mV or 1 V full scale Figure 30 Analog Output Settings 2 Not Ready Information Figure 31 Analog Output Settings 3 Change the values if required Agilent 1200 Infinity Series VWD User Manual 75 4 Using the Detector Special Setpoints 1 To change the offset and the attenuation select VWD
63. Signal gt More gt Special Setpoints WD Special Setpoints Instrument 2 xj QE Set up YWD Signal Signal Polarity can be switched to negative if required Negative Enable analysis when lamp is off if the VWD is not used in a dual detector setup lamp off the not ready condition is not stopping the analysis Scan Range Step Used for stop flow scanning Scan Range 190 to 400 nm Step 2 nm see Scanning with the VWD on page 74 aa Control Fu Online Spectra Signal Polarity 3 Not Ready Information Help I Enable analysis when lamp is off Restore Defaults Cancel Help Figure 32 Special Setpoints Peakwidth Settings Do not use peak width shorter than necessary see details below 1 To change the Peakwidth settings select Setup Detector Signals 2 In the section Peakwidth Responsetime click on the drop down list 3 Change the Peakwidth according to your needs 76 Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 x Peakwidth enables you to select the peak Heme peee Time width response time for your analysis The fu Online Spectra i peak width is defined as the width of a peak Tre oil ae in minutes at half the peak height Set the Help as no Lint 3 min peak width to the narrowest expected peak in Peakwidth Responsetime Posttime your chromatogram The peak width sets the gt O1min 2s X Off 4 min optimum res
64. Using the Agilent BootP Service Agilent BootP Service starts automatically when your PC reboots To change Agilent BootP Service settings you must stop the service make the changes and then restart the service Stopping the Agilent BootP Service 1 From the Windows control panel select Administrative Tools gt Services The Services screen appears RE Be aton yew teb s PDBIQ gt s n Select an tem to view ts description Figure 71 Windows Services screen 2 Right click Agilent BootP Service 3 Select Stop 4 Close the Services and Administrative Tools screen Agilent 1200 Infinity Series VWD User Manual 217 13 LAN Configuration Editing the IP address and other parameters in EditBootPSettings 1 Select Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 When the BootP Settings screen is first opened it shows the default settings from installation 3 Press Edit BootP Addresses to edit the Tab File x Host Name IP Address Comment Gateway 006000111999 Agier LC1 1011 10 Agiert LET ngtt 285 255 2550 0000 005000222883 Ager C2 101 1 1 102 Agiert LC2 left 252552550 0000 Figure 72 Edit BootP Adresses screen 4 In the Edit BootP Addresses screen press Add to create a new entry or select an existing line from the table and press Modify or Delete to change the IP address comment subnet mask for example in the Tab F
65. WD User Manual 10 Parts and Materials for Maintenance Overview of Maintenance Parts 150 Standard Flow Cell 10mm 14pL 152 Micro Flow Cell3mm 2 pL 154 Semi micro Flow Cell6mm 5pl 156 High Pressure Flow Cell 10mm 14pL 158 Cuvette Holder 160 Kits 151 Leak Parts 161 This chapter provides information on parts for maintenance Apg Agilent Technologies 149 10 Parts and Materials for Maintenance Overview of Maintenance Parts p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m G1314 60101 Deuterium lamp with RFID tag G1314 60186 Standard flow cell 10 mm 14 pL 40 bar with RFID tag G1314 60187 Micro flow cell 3 mm 2 uL 120 bar with RFID tag G1314 60183 Semi micro flow cell 6 mm 5 pL with RFID tag G1314 60182 High pressure flow cell 10 mm 14 pL 400 bar with RFID tag G1314 60200 Cuvette Holder 5067 4691 Front Panel DAD VWD FLD 1260 1290 5065 9982 Front Panel DAD VWD FLD 1200 For details on flow cells refer to 150 Standard Flow Cell 10 mm 14 uL on page 152 Micro Flow Cell 3 mm 2 uL on page 154 Semi micro Flow Cell 6 mm 5 uL on page 156 and High Pressure Flow Cell 10 mm 14 uL on page 158 Agilent 1200 Infinity Series VWD User Manual Kits HPLC System Tool Kit Parts and Materials for Maintenance 10 HPLC System Tool Kit G4203 68708 contains some accessories and tools needed for installati
66. a very rare case the capacity of the CompactFlash Card is not sufficient This could happen for example when the interrupt of LAN communication takes longer and the detector uses special settings e g full data rate at 80 Hz plus full spectra plus all signals during data buffering Probable cause Suggested actions 1 CompactFlash Card is full Correct communication problem Reduce data rate 112 Agilent 1200 Infinity Series VWD User Manual Error Information 7 Cover Violation Error ID 7461 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed while the lamps are on or if an attempt is made to switch on for example the lamps with the foam removed the lamps are switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative Agilent 1200 Infinity Series VWD User Manual 113 7 Error Information Detector Error Messages 114 Agilent 1200 Infinity Series VWD User Manual This chapter describes the detector s built in test functions Agilent 1200 Infinity Series VWD User Manual 8 Test Functions Intensity Test 116 Intensity Test Failed 117 Cell Test 118 Wavelength Verification Calibration 120 ASTM Drift and Noise Test 122 Quick Noise Test 12
67. acing firmware 147 147 RFID Radio Frequency Identification 10 RS 232C cable 1 74 run recovery automatic 81 manual 82 no run data available in 112 running the sample 70 S safety class 229 safety general information 229 standards 23 symbols 228 sample info 69 sampling rate data rate 30 27 24 scanning 74 serial number 181 181 setting up an analysis 57 information settings analog output settings 75 peakwidth 76 setup of detector 65 shutdown 98 signal diagnostic 90 plot 67 site requirements environment 22 power considerations 20 power cords 21 solvent information 234 sound emission 232 source lens assembly 13 special interfaces 188 Agilent 1200 Infinity Series VWD User Manual Index special setpoints 76 special settings boot resident 191 forced cold start 192 specification physical 23 specifications 26 29 32 noise and linearity 32 29 26 performance 24 physical 23 spectra online 73 tools 31 28 25 stable baseline 63 stack configuration front view 41 rearview 42 status indicators 90 91 status indicator 92 stepper motor 15 stop flow condition 73 T TCP IP parameter configuration 199 telnet configuration 220 temperature control configuration 72 temperature sensor 100 test functions 90 115 tests available tests vs interface 93 holmium oxide 126 intensity of deuterium lamp 116 wavelength calibration 120 timeout 98 tool kit hplc system 151 troubleshooting 24
68. age 39 and Figure 8 on page 40 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required The Agilent 1290 Infinity Binary Pump should always be installed at the bottom of the stack ofS o ooo 838 888 ao c c5 f j f SST Figure 7 Recommended stack configuration for 1290 Infinity with binary pump front view Agilent 1200 Infinity Series VWD User Manual 39 3 Installing the Detector Optimizing the Stack Configuration LAN to LC ChemStation INAV WINN CAN Bus cable to Instant Pilot Analog detector signal optional AC Power CAN Bus cable Figure 8 Recommended stack configuration 1290 Infinity with binary pump rear view 40 Agilent 1200 Infinity Series VWD User Manual Installing the Detector 3 Optimizing the Stack Configuration Two Stack Configuration Two Stack Configuration for Agilent 1260 Infinity LC To avoid excessive height of the stack when the autosampler thermostat is added to the system it is recommended to form two stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 9 on page 41 and Figure 10 on page 42 Instant Pilot f Irre Detector Column compartment
69. ata Analysis Resume Injection Run Sequence F6 r Data File Sample Info System 2 Operator Name wolfgang x Pause Sequence Resume Sequence Stop Runfinject Sequence Manual Filename fiso_o1 D wg DGCALAS M Prefix Counter Path JE CHEMSTATION 2 DATAS z Subdirectory ISOTEST m Sample Parameters Location vial 1 blank run if no entry Sample Name isocratic test sample Isocratic test sample 1 ul 30 70 H2O Acetonitrile 1 5 ml min Sample Amount 0 Multiplier fi ISTD Amount fo Dilution fi Comment P Run Method OK Cancel Help Figure 25 Sample Information 4 15 Fill the content of an isocratic standard sample ampoule into a vial and seal the vial with a cap and place the vial into autosampler tray position 1 Agilent 1200 Infinity Series VWD User Manual 4 Using the Detector Running the Sample and Verifying the Results 1 To start a run select the menu item RunControl gt Run Method 2 This will start the modules and the online plot on the Agilent ChemStation will show the resulting chromatogram DAD1 A Sig 254 4 Ref 500 100 041014410140003 D mau pot es ae 5 ST a a a a Da aa a ee he pate ae fp min Figure 26 Chromatogram with lsocratic Test Sample Information about using the Data Analysis functions can be obtained from the Using your ChemStation manual supplied w
70. bulences around one minute or less can be ignored Performance tests should be done with a completely warmed up optical unit gt one hour ASTM measurements require that the detector should be turned on at least 24 hours before start of testing 26 Agilent 1200 Infinity Series VWD User Manual Site Requirements and Specifications 2 Performance Specifications G1314E Table4 Performance Specifications G1314E Type Specification Comments Detection type Double beam photometer Light source Wavelength range Short term noise Drift Linearity Wavelength accuracy Maximum data rate Band width Flow cells Electronic Temperature Control ETC Deuterium lamp 190 600 nm 0 15 10 AU at 230 nm lt 1 104 AU h at 230 nm gt 2 5 AU 5 at 265 nm 1nm 160 Hz 6 5 nm typical Standard 14 pL volume 10 mm cell path length and 40 bar 588 psi pressure maximum High pressure 14 uL volume cell path length and 400 bar 5880 psi pressure maximum Micro 2 uL volume 3 mm cell path length and 120 bar 1760 psi pressure maximum Semi micro 5 pL volume 6 mm cell path length and 40 bar 588 psi pressure maximum For improved baseline stability in instable environment The UV lamp is equipped with RFID tag that holds lamp typical information Under specified condtions See Specification Conditions G1314E on page 29 below the table Under specified condtions See Specification
71. condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 191 Then try a firmware update see Replacing the Module s Firmware on page 147 If this does not help a main board replacement is required Agilent 1200 Infinity Series VWD User Manual Available Tests versus Interfaces Troubleshooting and Diagnostics 6 Depending on the used interface the available tests and the screens reports may vary Preferred tool should be the Agilent Lab Advisor see Agilent Lab Advisor Software on page 94 In future a user interface may not show the Diagnostics Tests anymore Then the Agilent Lab Advisor must be used instead The Agilent ChemStation may not include any maintenance test functions Table 15 Available Tests versu
72. crease is not detected the error message is generated Probable cause Suggested actions 1 Motor is not connected Please contact your Agilent service representative 2 Defective motor Please contact your Agilent service representative 3 Defective missing grating or filter Please contact your Agilent service representative 4 Cable connector defective Please contact your Agilent service representative Agilent 1200 Infinity Series VWD User Manual 109 7 Error Information ADC Hardware Error Error ID 7830 7831 A D Converter hardware is defective Probable cause Suggested actions 1 A D Converter hardware is defective Please contact your Agilent service representative Illegal Temperature Value from Sensor on Main Board Error ID 1071 This temperature sensor located on the detector main board delivered a value outside the allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause Suggested actions 1 Defective sensor or main board Please contact your Agilent service representative 2 Detector is exposed to illegal ambient Verify that the ambient conditions are within conditions the allowed range 110 Agilent 1200 Infinity Series VWD User Manual Error Information 7 Illegal Temperature Value from Sensor at Air Inlet Error ID 1072 This temperature sensor delivered a value outside the allowed range
73. d 105 amp voltage missing 105 eak sensor open 101 eak sensor short 101 eak 100 ost CAN partner 100 no run data available in device 112 remote timeout 99 shutdown 98 timeout 98 wavelength check failed 109 F fan failed 103 features GLP 31 28 25 safety and maintenace 31 28 25 firmware description 176 main system 1 6 resident system 176 update tool 177 updates 177 147 147 upgade downgrade 147 upgrade downgrade 147 flow cell configuration 72 correction factors 87 high pressure parts 158 micro parts 154 semimicro parts 156 standard parts 152 types anddata 30 27 24 with RFID tag 12 flow cells matching for application 85 Agilent 1200 Infinity Series VWD User Manual Index frequency range 23 G general error messages 98 grating assembly 15 holmium oxide declaration of conformity 236 filter 14 test 126 humidity 23 l information on cuvette holder 143 on solvents 234 on sound emission 232 on UV radiation 233 initialization mode selection 201 installation bench space 22 of flow connections 52 of the detector 49 Instant Pilot G4208A 10 instrument layout 193 interfaces 182 internet 237 introduction 10 to optical system 11 L lamp configuration 72 initial drift 68 intensity test 116 type 30 27 24 with RFIDtag 13 239 Index LAN automatic configuration with Bootp 209 Bootp amp Store 202 Bootp 201 cable 173 configuration switches 200
74. d Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low Agilent 1200 Infinity Series VWD User Manual 171 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module 1 1 BCD 5 20 2 2 BCD 7 80 Be 15 Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s S e 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 172 Agilent 1200 Infinity Series VWD User Manual CAN LAN Cables Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Agilent 1200 Infinity Series VWD User Manual 173 11 Identifying Cables RS 232 Cables 174 p n G1530 60600 RS232 61601 5181 1561 Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cabl
75. d flow cell for fast replacement electronic identification of flow cell and lamp with RFID Radio Frequency Identification tag for unambiguous identification lamp information part number serial number production date ignitions burn time cell information part number serial number production date nominal path length volume maximum pressure built in electronic temperature control ETC for improved baseline stability and built in holmium oxide filter for fast wavelength accuracy verification For specifications refer to Table 3 on page 24 10 Agilent 1200 Infinity Series VWD User Manual Introduction to the Variable Wavelength Detector 1 Optical System Overview The optical system of the detector is shown in the figure below Its radiation source is a deuterium arc discharge lamp for the ultraviolet UV wavelength range from 190 to 600 nm The light beam from the deuterium lamp passes through a lens a filter assembly an entrance slit a spherical mirror M1 a grating a second spherical mirror M2 a beam splitter and finally through a flow cell to the sample diode The beam through the flow cell is absorbed depending on the solutions in the cell in which UV absorption takes place and the intensity is converted to an electrical signal by means of the sample photodiode Part of the light is directed to the reference photodiode by the beam splitter to obtain a reference signal for compensation of intensity fluc
76. d optimizing e troubleshooting and diagnose maintenance and repair parts identification e hardware information e safety and related information Agilent Technologies 2008 2010 2012 2013 Printed in Germany 11 2013 G1314 90034 Rev B EE Agilent Technologies
77. d reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Eye damage by detector light 0 Eye damage may result from directly viewing the UV light produced by the lamp of the optical system used in this product gt Always turn the lamp of the optical system off before removing it WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module Agilent 1200 Infinity Series VWD User Manual 131 9 Maintenance and Repair Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent prod
78. depends on the data load Run in progress Data Analysis WWD Status Prerun Rawdata REMAN Rawdats Elapsed run time stops a Wavelength 254 nm ChemStation continues to add missing data Ready wo status Run finished o Peun Ready Prerun Ready Wavelength 254 nm If the detector status window is not opened you will realize only the Power Fail error and the long Run In Progress information until the data is recovered from disk Agilent 1200 Infinity Series VWD User Manual 81 4 Using the Detector Special Settings of the Detector Manual Run Recovery in case of permanent communication failures DAD Run Recovery G1315C see Note below 2 Mot Ready Information Start a recover DAD Run Recovery G1315C After a recover DAD Run Recovery G1315C NOTE When during recovery an error Method Sequence stopped appears the instrument logbook shows an entry No Run data available in device In this case refer to No Run Data Available In Device on page 112 82 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual ee 5 r How to optimize the detector Optimizing the Detector Performance 84 Match the Flow Cell to the Column 85 Set the Detector Parameters 88 This chapter gives hints on how to select the detector parameters and the flow cell ott Agilent Technologies 83 5 Howto optimize
79. e Micro Flow Cell 3 mm 2 uL on page 154 Semi micro Flow Cell 6 mm 5 pL on page 156 e High Pressure Flow Cell 10 mm 14 uL on page 158 Preparations Turn the lamp OFF 1 Press the release buttons and remove the front cover to 2 Disconnect the inlet and outlet capillaries have access to the flow cell area 138 Agilent 1200 Infinity Series VWD User Manual Maintenance and Repair 9 3 Unscrew both thumb screws parallel and remove the flow cell If you want to maintain flow cell parts see Overview of Maintenance Parts on page 150 or the information provided with your flow cell 4 Replace the flow cell and fix the thumb screws Reconnect the inlet and outlet capillaries to the flow cell 5 Replace the front cover Next Steps 6 To check for leaks establish a flow and observe the flow cell outside of the cell compartment and all capillary connections Insert the flow cell Perform Wavelength Verification Calibration on page 120 to check the correct positioning of the flow cell 9 Replace the front cover Agilent 1200 Infinity Series VWD User Manual 139 9 Maintenance and Repair Repairing the Flow Cells Parts required Description 1 Flow cell For details on flow cells see Standard Flow Cell 10 mm 14 uL on page 152 Micro Flow Cell 3 mm
80. e representative Please contact your Agilent service representative 101 7 102 Error Information Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Agilent 1200 Infinity Series VWD User Manual Error Information 7 Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board
81. e with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 12 Hardware Information Firmware Description 176 Electrical Connections 179 Rear View of the Module 180 Information on Instrument Serial Number 181 Interfaces 182 Overview Interfaces 185 Setting the 8 bit Configuration Switch 189 Special Settings 191 Instrument Layout 193 Early Maintenance Feedback EMF 194 EMF Counter 194 Using the EMF Counters 195 This chapter describes the detector in more detail on hardware and electronics ott Agilent Technologies 175 12 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections e a non instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument function
82. e BootP service Add the BootP service as an exception e Is the LAN Interface using the BootP mode instead of Using Stored or Using Default modes Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Installation of BootP Service Before installing and configuring the Agilent BootP Service be sure to have the IP addresses of the computer and instruments on hand 1 2 3 Log on as Administrator or other user with Administrator privileges Close all Windows programs Insert the Agilent ChemStation software DVD into the drive If the setup program starts automatically click Cancel to stop it 4 Open Windows Explorer 5 Go to the BootP directory on the Agilent ChemStation DVD and double click BootPPackage msi If necessary click the Agilent BootP Service icon in the task bar The Welcome screen of the Agilent BootP Service Setup Wizard appears Click Next The End User License Agreement screen appears Read the terms indicate acceptance then click Next The Destination Folder selection screen appears Install BootP to the default folder or click Browse to choose another location Click Next The default location for installation is C Program Files Agilent BootPService 10 Click Install to begin installation Agilent 1200 Infinity Series VWD User Manual 211 13 LAN Configuration 11 Files load when finished the BootP Settings screen appears BootP Settings a BootP Tab File
83. e with the standard e g NIST certified holmium oxide sample Preparations Remove the normal flow cell Have cuvette with standard available Agilent 1200 Infinity Series VWD User Manual 143 9 Maintenance and Repair 1 Locate the cuvette holder on the desk 2 Unscrew the bracket 3 Insert the cuvette with the sample into the holder The clear side of the cuvette must be visible Light path Clear side 4 Replace the bracket and fix the cuvette Next Steps 5 Install the cuvette holder in the instrument 6 Perform your Wavelength Verification Calibration Wavelength Verification Calibration on page 120 to check the correct position of the cuvette holder 144 Agilent 1200 Infinity Series VWD User Manual Maintenance and Repair 9 Correcting Leaks Correcting Leaks When If a leakage has occurred in the flow cell area or at the capillary connections Tools required Description Tissue Wrench 1 4 inch for capillary connections 1 Remove the front cover 2 Use tissue to dry the leak sensor area Observe the capillary connections and the flow cell area for leaks and correct if required 4 Replace the front cover Waste outlet eak sensor assembly Figure 48 Drying the Leak Sensor Agilent 1200 Infinity Series VWD User Manual 145 9 Maintenance and Repair Replacing Leak Handling System Parts When Tools required
84. e8 What you will need Agilent 1200 Pump plus degassing Infinity Series system Autosampler Detector standard flow cell installed Degasser optional Column Compartment optional Agilent ChemStation or Instant Pilot G4208 optional for basic operation System should be correctly set up for LAN communication with the Agilent ChemStation Column Zorbax Eclipse XDB C18 4 6 x 150 mm 5 pm 993967 902 or an equivalent column Standard Agilent isocratic checkout sample 01080 68704 Conditions A single injection of the isocratic test standard is made under the conditions given in Table 9 on page 59 Table9 Conditions Flow 1 5 mL min Stoptime 8 min Solvent 100 30 water 70 Acetonitrile Temperature Ambient Wavelength sample 254 nm Injection Volume Tul Column Temperature optional 25 C or ambient Agilent 1200 Infinity Series VWD User Manual 59 4 Using the Detector Typical Chromatogram A typical chromatogram for this analysis is shown in Figure 16 on page 60 The exact profile of the chromatogram will depend on the chromatographic conditions Variations in solvent quality column packing standard concentration and column temperature will all have a potential effect on peak retention and response DAD1 A Sig 254 4 Ref 500 100 04101410140003 D mAU yt a he gL a e EE Tm CC Cm CC CC E min Figure 16 Typical Chromatogram with UV de
85. ecords of maintenance and errors Verification of wavelength accuracy with built in holmium oxide filter RFID for electronics records of flow cell and UV lamp conditions path length volume product number serial number test passed usage Housing All materials recyclable 28 Agilent 1200 Infinity Series VWD User Manual Site Requirements and Specifications 2 Specification Conditions G1314E ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions Standard flow cell path length 10 mm flow 1 mL min LC grade methanol Noise 0 15 10 AU at 230 nm TC 2 s RT 2 2 TC Linearity Linearity is measured with caffeine at 265 nm The specification are based on the the standard RFID tag lamp G1314 60101 and may be not achieved when other lamp types or aged lamps are used ASTM drift tests require a temperature change below 2 C hour 3 6 F hour over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Performance tests should be done with a completely warmed up optical unit
86. eed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 228 Agilent 1200 Infinity Series VWD User Manual Appendix 14 General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals exte
87. er at fan assembly failed 111 Heater Power At Limit 112 Apg Agilent Technologies 95 7 Error Information No Run Data Available In Device 112 Cover Violation 113 This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from error conditions 96 Agilent 1200 Infinity Series VWD User Manual Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by
88. erature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 0 55 C 32 131 F 40 70 C 40 158 F lt 95 rh at 40 C 104 F Up to 2000 m 6562 ft Up to 4600 m 15091 ft Installation category II Pollution degree 2 Non condensing For storing the module For indoor use only Agilent 1200 Infinity Series VWD User Manual 23 2 Site Requirements and Specifications Performance Specifications G1314D Performance Specifications G1314D Table 3 Performance Specifications Type Specification Comments Detection type Double beam photometer Light source Wavelength range Short term noise Deuterium lamp 190 600 nm 0 15 10 AU at 230 nm The UV lamp is equipped with RFID tag that holds lamp typical information Under specified condtions See Specification Conditions G1314D on page 26 below the table Drift lt 1 104 AU h at 230 nm Under specified condtions See Specification Conditions G1314D on page 26 below the table Linearity gt 2 5 AU 5 at 265 nm Under specified condtions See Specification Conditions G1314D on page 26 below the table Wavelength 1nm Self calibration with deuterium lines accuracy verification with holmium oxide filter Maximum sampling 20 Hz rate Band width 6 5 nm typical Flow cells Standard 14 pL volume 10 mm cell pathlength All flow
89. est settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 e For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode For normal operation use the default best settings AN CONFIG CAN CAN j USB J 7 a A Ie A SO i O f D Aylan Tecdnsioges Figure 59 Location of Configuration Switch example shows a G4212A DAD Agilent 1200 Infinity Series VWD User Manual 189 12 Hardware Information To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration Table 21 8 bit Configuration Switch with on board LAN Mode Function swt sw2 sw3 sw4 sws swe sw7 sws LAN 0 0 Link Configuration Init Mode Selection Auto negotiation 0 x xX x x x 10 MBit half duplex 1 0 0 x X xX 10 MBit full duplex 1 0 1 x xX X 100 MBit half duplex 1 1 0 X xX xX 100 MBit full duplex 1 1 1 x X X Bootp x X x 0 0 0 Bootp amp Store x x x 0 0 1 Using Stored x x x 0 1 0 DHCP x xX x 1 0 0 Using Default x X x 0 1 1 TEST 1 1 System NVRAM Boot Resident System 1 X Revert to Default Data Coldstart x Xx Xx 1 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Re
90. etely This is required to avoid any problem with helicoil insert in the casting 3 Press the flow cell completely into the slot and tighten the cell screws both parallel until the mechanical stop 4 Assemble the column detector capillary Depending on the flow cell type it may be a PEEK or SST capillary Agilent 1200 Infinity Series VWD User Manual 53 3 Installing the Detector 5 Connect the newly assembled fitting of the capillary to 6 Connect the PEEK waste capillary to the outlet the inlet connector and connect the other end of the connector capillary to the column 8 Replace the front cover The installation of the detector is now complete The detector should be operated with the front cover in place to protect the flow cell area against strong drafts from the outside 54 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 00 ee 4 7 e Using the Detector amp Leak and Waste Handling 56 Setting up an Analysis 57 Before Using the System 57 Requirements and Conditions 59 Optimization of the System 61 Preparing the HPLC System 61 Running the Sample and Verifying the Results 70 Special Settings of the Detector 71 Control Settings 71 Configuration Settings 72 Online Spectra 73 Scanning withthe WWD 74 Analog Output Settings 75 Specia
91. ew on page 164 Instant Pilot G4208A is optional Preparations Other LC modules must have appropriate firmware installed to work with the detector Locate bench space Provide power connections Unpack the detector Before adding the detector into an existing system assure that the existing modules have been updated to firmware revision that is supported by the control software If you want to use the data never lost feature then assure that the CompactFlash Card is installed in the rear of the G1314E VWD Agilent 1200 Infinity Series VWD User Manual 49 3 Installing the Detector 1 Note the MAC address of the LAN interface rear of the module under the configuration switch see figure below It s required for LAN Configuration see chapter LAN Configuration REMOTE 5 gt IK IC gt SS gp CONFIG CAHN Rg 282 o LAN LR Co k TT 1314 65080 vee Part number of board B 01 ZZ 0444 Revision Code Vendor Year and Week of assembly MAC 000001000001 MAC address LL Country of Origin Figure 15 Rear View of Detector 2 Check the setting of the DIP switch at the rear of the detector All switches should be in down position BOOTP mode If another boot mode is required refer
92. g the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 99 7 100 Error Information Lost CAN Partner Leak Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Bro
93. gilent 1200 Infinity Series VWD User Manual 10 Parts and Materials for Maintenance 149 Overview of Maintenance Parts 150 Standard Flow Cell10mm 14uL 152 Micro Flow Cell3mm 2puL 154 Semi micro Flow Cell6mm 5uL 156 High Pressure Flow Cell 10mm 14pL 158 Cuvette Holder 160 Kits 151 Leak Parts 161 1 Identifying Cables 163 Cable Overview 164 Analog Cables 166 Remote Cables 168 BCD Cables 171 CAN LAN Cables 173 RS 232 Cables 174 12 Hardware Information 175 Firmware Description 176 Electrical Connections 179 Interfaces 182 Setting the 8 bit Configuration Switch 189 Instrument Layout 193 Early Maintenance Feedback EMF 194 13 LAN Configuration 197 What you have to do first 198 TCP IP parameter configuration 199 Configuration Switches 200 Initialization mode selection 201 Dynamic Host Configuration Protocol DHCP 205 Link configuration selection 208 Automatic configuration with Bootp 209 Manual Configuration 219 PC and User Interface Software Setup 225 Agilent 1200 Infinity Series VWD User Manual Contents Contents 14 Appendix 227 General Safety Information 228 Radio Interference 231 Sound Emission 232 UV Radiation 233 Solvent Information 234 Declaration of Conformity for HOX2 Filter 236 Agilent Technologies on Internet 237 8 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 00 ee 1 7 e Introduction to the Variable Wavelength
94. gilent 1200 Infinity Series VWD User Manual 51 3 Installing the Detector Flow Connections to the Detector Tools required Parts required Hardware required Preparations Description Wrench 1 4 5 16 inch for capillary connections p n Description 1 G1314 68755 Accessory kit Other modules depend on system setup Detector is installed in the LC system WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 52 The flow cell is shipped with a filling of isopropanol also recommended when the instrument and or flow cell is shipped to another location This is to avoid breakage due to subambient conditions Agilent 1200 Infinity Series VWD User Manual Installing the Detector 3 1 Press the release buttons and remove the front cover to have access to the front area 2 Loose the screws of the flow cell dummy plate by turning each screw one turn Then unscrew the screws compl
95. greement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or asa commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to
96. gure 11 Recommended two stack configuration for 1290 Infinity with binary pump front view Agilent 1200 Infinity Series VWD User Manual 43 3 Installing the Detector Optimizing the Stack Configuration LAN to LC ChemStation CAN Bus cable to Instant Pilot Analog detector signal tional optional EN i PA CAN Bus cable Thermo cable optional AC Power Figure 12 Recommended two stack configuration for 1290 Infinity with binary pump rear view 44 Agilent 1200 Infinity Series VWD User Manual Installing the Detector 3 Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the max
97. he following at the system DOS prompt c gt telnet lt IP address gt or c gt telnet lt host name gt cx C WINDOWS system32 cmd exe C gt telnet 134 40 27 104 Figure 74 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle a configuration session with the Local Controller or the default IP address see Configuration Switches on page 200 When the connection was established successfully the module responds with the following cx Telnet 134 40 27 104 Agilent Technologies Gi314E DE81966662 Figure 75 A connection to the module is made 3 Type and press enter to see the available commands cv Telnet 134 40 27 104 P Technologies G1314E DE81900002 syntax description display help info display current LAN settings set IP Address set Subnet Mask set Default Gateway exit shell Figure 76 Telnet Commands 220 Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Table 30 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X xX gt sets new ip address SM lt X X X X gt sets new subnet mask gw lt X X X x gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 27 230 Then press Enter where parameter refers to the configuration parame
98. he status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 7 Error Information What Are ErrorMessages 97 General Error Messages 98 Timeout 98 Shutdown 98 Remote Timeout 99 Lost CAN Partner 100 Leak 100 Leak Sensor Open 101 Leak Sensor Short 101 Compensation Sensor Open 102 Compensation Sensor Short 102 Fan Failed 103 Open Cover 103 Detector Error Messages 104 UV lamp no current 104 UV lamp no voltage 105 Ignition Failed 105 No heater current 106 Wavelength calibration setting failed 106 Wavelength holmium check failed 107 Grating or Filter Motor Errors 108 Wavelength test failed 109 Cutoff filter doesn t decrease the light intensity at 250nm 109 ADC Hardware Error 110 Illegal Temperature Value from Sensor on Main Board 110 Illegal Temperature Value from Sensor at Air Inlet 111 Heat
99. his connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1200 Infinity Series VWD User Manual 179 12 Hardware Information Electrical Connections Rear View of the Module SE REMOTE e a a a J Sc 7c oe O e223 O at iss RS 232 TC BEE 0 e mao cme nae ane SSS N a e E Figure 57 Rear View of Detector NOTE The Compact Flash card slot is used for the G1314E VWD only 180 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Information on Instrument Serial Number Serial Number Information 1200 Series and 1290 Infinity The serial number information on the instrument labels provide the following information CCYWWSSSSS Format CC country of manufacturing DE Germany e JP Japan e CN China YWW year and week of last major manufacturing change e g 820 could be week 20 of 1998 or 2008
100. ications Noise Test 122 noise short term 24 27 30 2 298 26 non operating altitude 23 non operating temperature 23 0 online plot 68 spectra 73 operating Altitude 23 operating temperature 23 optical unit beam splitter assembly 15 entrance slit assembly 13 ilter assembly 14 filter 14 flow cell 12 grating assembly 15 lamp 13 mirrors 15 photodiode assemblies 15 photodiode boards 15 source lens assembly 13 stepper motor 15 i optimization detector performance 84 ofthe system 61 overview optical path 11 optical system 11 system overview 11 P packaging damaged 34 parameters detector 65 parts for maintenance cuvette holder 160 high pressure flow cell 158 leak parts 161 micro flow cell 154 Agilent 1200 Infinity Series VWD User Manual semimicro flow cell 156 standard flow cell 152 parts and materials for maintenance 149 PC and User Interface Software Setup 225 peakwidth settings 76 performance optimization 84 specifications 24 photodiode assemblies 15 boards 15 photometric accuracy 87 physical specifications 23 physical specifications 23 power consumption 23 power cords 21 power considerations 20 preparing the HPLC system 61 Q quick noise test 123 Radio Frequency Identification flow cell and lamp 10 radio interference 231 recalibration of wavelength 90 remote cable 168 repairs cautions and warnings 131 introduction 129 overview of simple repairs 133 repl
101. igh Pressure Flow Cell 10 mm 14 pL Item p n Description G1314 60182 High pressure flow cell 10 mm 14 pL 400 bar with RFID tag G1315 87311 Capillary ST 0 17 mm x 380 mm S S 1 G1314 20047 Cell screw G1314 65054 Cell kit Agilent comprises two windows two KAPTON gaskets and two PEEK rings 2 Ring PEEK kit 3 Window quartz kit 4 Gasket kit KAPTON 5 G1314 44010 Clip for RFI ID tag 6 0515 4780 Screw for Clip M2 2 4 5 mm long 158 Agilent 1200 Infinity Series VWD User Manual Parts and Materials for Maintenance 10 High Pressure Flow Cell 10 mm 14 pL Cell screw N Ring PEEK wo Quartz window 4 Gasket KAPTON 5 RFID Clip 6 Screw Figure 53 High Pressure Flow Cell Agilent 1200 Infinity Series VWD User Manual 159 10 Parts and Materials for Maintenance Cuvette Holder For information the use of the cuvette holder refer to Using the Cuvette Holder on page 143 p n Description G1314 60200 Cuvette Holder Figure 54 Cuvette Holder 160 Agilent 1200 Infinity Series VWD User Manual Leak Parts Item p n 3 5041 8388 4 5041 8389 5 5041 8387 6 5062 2463 7 5062 2463 Parts and Materials for Maintenance 10 Leak Parts Description Leak funnel Leak funnel holder Tube clip Corrugated tubing PP 6 5 mm id 5m Corrugated tubing PP 6 5 mm id 5m Figure 55 Leak Parts Agilent 1200 Infinity Series VWD User Manual 161 10 Parts and Materials for Maintenance
102. ile If you change the IP address it will be necessary to power cycle the instrument for the changes to take effect 5 Leave Edit BootP Addresses by pressing Close 6 Exit BootP Settings by pressing OK Restarting the Agilent BootP Service 1 In the Windows control panel select Administrative Tools gt Services The Services screen appears see Figure 71 on page 217 2 Right click Agilent BootP Service and select Start 3 Close the Services and Administrative Tools screens 218 Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Non Volatile RAM Stored Parameter Ld Control Module Figure 73 Manual Configuration Principle Agilent 1200 Infinity Series VWD User Manual 219 13 LAN Configuration With Telnet Whenever a TCP IP connection to the module is possible TCP IP parameters set by any method the parameters may be altered by opening a Telnet session 1 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and press OK 2 Type t
103. imum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets Agilent 1200 Infinity Series VWD User Manual 45 3 Installing the Detector Installation Information on Leak and Waste Handling Figure 13 Leak and waste handling overview typical stack configuration as an example 46 Agilent 1200 Infinity Series VWD User Manual Installing the Detector 3 1 Solvent cabinet Leak pan 3 Leak pan s outlet port A leak funnel B and corrugated waste tube C 4 Waste tube of the sampler s needle wash 5 Condense drain outlet of the autosampler cooler 6 Waste tube of the purge valve 7 Waste tube 1 Stack the modules according to the adequate stack configuration The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module see Figure 13 on page 46 2 Connect data and power cables to the modules see section Installing the Module below 3 Connect capillaries and tubes to the modules see section Flow Connections to the module below or the relevant system manual
104. ining strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1200 Infinity Series VWD User Manual 235 14 Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter 236 We herewith inform you that the Declaration of Conformity Holmium Oxide Glass Filter used in Agilents absorbance detectors listed in the table below meets the requirements of National Institute of Standards and Technology NIST to be applied as certified wavelength standard According to the publication of NIST in J Res Natl Inst Stand Technol 112 303 306 2007 the holmium oxide glass filters are inherently stable with respect to the wavelength scale and need no recertification The expanded uncertainty of the certified wavelength values is 0 2 om Agilent Technologies guarantees as required by NIST that the material of the filters is holmium oxide glass representing the inherently existent holmium oxide absorption bands Test wavelengths Product Number 79883A 79854A G1306A G1315A G1365A G1315B C G1365B C G1600A G7100A G1314A B C G1314D EF G4286 90A B C 1120 1220 Wavelength Optical Accuracy Bandwidth The variation in Measured Wavelength depends on the different Optical Bandwidth P N 89550 90501 May 19 2010 Agilent 1200 Infinity Series VWD User Manual
105. inity Series VWD User Manual Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Remote Timeout Error ID 0070 Error Information 7 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Agilent 1200 Infinity Series VWD User Manual Suggested actions Ensure the instrument showin
106. ion DVD The Agilent BootP Service is installed on a server or PC on the LAN to provide central administration of IP addresses for Agilent instruments on a LAN The BootP service must be running TCP IP network protocol and cannot run a DHCP server Agilent 1200 Infinity Series VWD User Manual 209 13 LAN Configuration 210 How BootP Service Works When an instrument is powered on an LAN Interface in the instrument broadcasts a request for an IP address or host name and provides its hardware MAC address as an identifier The Agilent BootP Service answers this request and passes a previously defined IP address and host name associated with the hardware MAC address to the requesting instrument The instrument receives its IP address and host name and maintains the IP address as long as it is powered on Powering down the instrument causes it to lose its IP address so the Agilent BootP Service must be running every time the instrument powers up If the Agilent BootP Service runs in the background the instrument will receive its IP address on power up The Agilent LAN Interface can be set to store the IP address and will not lose the IP address if power cycled Situation Cannot Establish LAN Communication If a LAN communication with BootP service cannot be established check the following on the PC e Is the BootP service started During installation of BootP the service is not started automatically Does the Firewall block th
107. ith your system 70 Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 Special Settings of the Detector In this chapter special settings of the detector are described Control Settings set up WHO Sina x Lamp gt _ Enror Method gt Lamp turn on and off of UV lamp tuOnline Spectra ere At Power On automatic lamp on at power on Not Ready Information Pie Take current method Error Method take error method or current method in pil ES case of an error Analog Output Range can be set to either 100 mV or M Analog Output Range in 1 V full scale see Analog Output Settings on At Power On rine CAN page 75 J Tum lamp on Automatic Turn On lamps can be programmed detector must be on for this Automatic Turn On r Help online help I Turn lamp on at Date 21 05 2008 lt ddimmyyyy Time 155647 lt hh mmiss Cancel Help Figure 27 Detector control settings Agilent 1200 Infinity Series VWD User Manual 71 4 Using the Detector Configuration Settings Instrument Method Sequence View Abort Help Set up ViWD Signals Se Control Snapshot Configuration Sample Sca System On Blank Sca HANK Je System Off i D Configuration Instrument 2 x M Temperature control Temperature control on Temperature control off gt U lamp Tag Automatic Mode only if RFI
108. ken capillary Exchange defective capillaries 3 Leaking flow cell Exchange flow cell components Agilent 1200 Infinity Series VWD User Manual Leak Sensor Open Error ID 0083 Error Information 7 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component Leak Sensor Short Error ID 0082 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective leak sensor 2 Leak sensor incorrectly routed being pinched by a metal component Agilent 1200 Infinity Series VWD User Manual Suggested actions Please contact your Agilent servic
109. l Repeat the test with the flow cell removed If the test is OK exchange the flow cell components 3 Dirty or defective holmium oxide filter Run the holmium oxide filter test If the test fails contact your Agilent service representative 4 Optical misalignment Please contact your Agilent service representative Agilent 1200 Infinity Series VWD User Manual 127 8 Test Functions Holmium Oxide Test 128 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 9 Maintenance and Repair Introduction to Maintenance 130 Warnings and Cautions 131 Overview of Maintenance 133 Cleaning the Module 134 ExchangingaLamp 135 Exchanging a Flow Cell 138 Repairing the Flow Cells 140 Using the Cuvette Holder 143 Correcting Leaks 145 Replacing Leak Handling System Parts 146 Replacing the Module s Firmware 147 This chapter provides general information on maintenance and repair of the detector Apg Agilent Technologies 129 9 Maintenance and Repair Introduction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack There are no serviceable parts inside Do not open the module 130 Agilent 1200 Infinity Series VWD User Manual Maintenance and Repair 9 Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples an
110. l Setpoints 76 Run Recovery G1314E 80 This chapter provides information on how to set up the detector for an analysis and explains the basic settings eh Agilent Technologies 55 4 Using the Detector Leak and Waste Handling WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regular
111. l instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or Lwhen between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Agilent 1200 Infinity Series VWD User Manual 187 12 Hardware Information Table 20 Remote Signal Dis
112. l is reached The leak pan s outlet port 8 A guides excessive overfill from one module to the next as the solvent flows into the next module s leak funnel 8 B and the connected corrugated waste tube 3 C The corrugated waste tube guides the solvent to the next lower positioned module s leak tray and sensor The waste tube of the sampler s needle wash port 4 guides solvents to waste The condense drain outlet of the autosampler cooler 5 guides condensate to waste The waste tube of the purge valve 6 guides solvents to waste The waste tube connected to the leak pan outlet on each of the bottom instruments 7 guides the solvent to a suitable waste container Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 00 e 7 e Site Requirements and Specifications amp i Site Requirements 20 Physical Specifications 23 Performance Specifications G1314D 24 Performance Specifications G1314E 27 Performance Specifications G1314F 30 This chapter gives information on environmental requirements physical and performance specifications ott Agilent Technologies 19 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Consideration The detector power supply has wide ranging capabilities see Physical Specifications on page 23 It accepts any line v
113. ly gt To achieve maximal safety check the correct installation regularly 56 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets For details on correct installation see Installation Information on Leak and Waste Handling on page 45 Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 Setting up an Analysis This chapter can be used for e preparing the system e to learn the set up of an HPLC analysis and to use it as an instrument check to demonstrate that all modules of the system are correctly installed and connected It is not a test of the instrument performance e Learn about special settings Before Using the System Solvent Information Observe recommendations on the use of solvents in chapter Solvents in the pump s reference manual Priming and Purging the System When the solvents have been exchanged or the pumping system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel between the solvent reservoir vacuum degasser when available in the system and the pump Solvents containing volatile ingredients will slightly lose these Therefore priming of the pumping system is required before starting an application Agilent 1200 Infinity Series VWD User Manual 57 4 Using the Detector Table 7 Choice of Priming Solvents for Different Pu
114. mation Early Maintenance Feedback EMF Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counter The detector module provides an EMF counter for the lamp The counter increments with lamp use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Dependig on the lamp type the counter can be reset to zero after the lamp is exchanged Lamp Type Counter Reset Comment lamp with RFID tag NO lamp without RFID tag YES via Lab Advisor or Instant Pilot The detector provides the following EMF counters e Deuterium Lamp On Time e Number of UV lamp ignitions 194 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Using the EMF Counters The user settable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The useful lamp burn time is dependent on the requirements for the analy
115. nsation sensor open 102 compensation sensor short 102 condensation 22 configuration switch location 200 configuration one stack 37 37 39 RFID tag lamp and cell 72 stack 36 temperature control 72 two stack front 43 two stack rear 44 twostack 41 43 control and data evaluation 25 28 31 correction factors for flow cells 87 cutoff filter 14 cuvette holder 143 parts 160 D data rate sampling rate 30 27 24 data recovery DRC 80 declaration of conformity 236 defect on arrival 34 Agilent 1200 Infinity Series VWD User Manual delivery checklist 35 detection type 24 27 30 detector error messages 104 DHCP general information 205 setup 206 diagnostic signals 90 test functions 115 dimensions 23 DRC runrecovery 80 drift 24 27 30 63 initial 68 E electrical connections descriptions of 179 EMF early maintenance feedback 194 setting limits 195 using counters 195 entrance slit assembly 13 environment 22 error message ADC hardware error 110 error messages calibration failed 106 compensation sensor open 102 compensation sensor short 102 detector 104 fan failed 103 filter check failed 109 grating filter motor defective 108 heater current missing 106 heater failed 111 heater power at limit 112 holmium oxide test failed 107 ignition without cover 103 103 illegal temperature value from sensor at air inlet 111 illegal value from sensor on main board 110 amp current missing 104 amp ignition faile
116. nsferred to the detector main board The photo diode ADC boards are located close to the photo diodes Agilent 1200 Infinity Series VWD User Manual 15 1 Introduction to the Variable Wavelength Detector System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed 16 Agilent 1200 Infinity Series VWD User Manual Introduction to the Variable Wavelength Detector 1 System Overview Figure 4 Leak and waste handling concept overview typical stack configuration as an example The solvent cabinet 1 is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 5 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet Agilent 1200 Infinity Series VWD User Manual 17 1 18 Introduction to the Variable Wavelength Detector The leak pan 2 individually designed in each module guides solvents to the front of the module The concept covers also leakages on internal parts e g the detector s flow cell The leak sensor in the leak pan stops the running system as soon as the leak detection leve
117. nsion cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for Agilent 1200 Infinity Series VWD User Manual 229 14 Appendix 230 replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a defini
118. nt is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 34 Agilent 1200 Infinity Series VWD User Manual Installing the Detector 3 Delivery Checklist Ensure all parts and materials have been delivered with the detector The delivery checklist is shown below Please report missing or damaged parts to your local Agilent Technologies sales and service office Table 6 Variable Wavelength Detector Checklist Description Quantity Variable wavelength detector 1 Power cable 1 Flow cell As ordered User Manual on Documentation CD part of the 1 per order shipment not module specific Accessory kit 1 CompactFlash Card G1314E 1 Detector Accessory Kit Contents The module is shipped with Accessory kit G1314 68755 see Accessory Kit on page 151 Agilent 1200 Infinity Series VWD User Manual 35 3 Installing the Detector Optimizing the Stack Configuration If your detector is part of a complete Agilent 1200 Infinity Series system you can ensure optimum performance by installing the following configuration This configuration optimizes the system flow path ensuring minimum delay volume 36 Agilent 1200 Infinity Series VWD User Manual Installing the Detector 3 Optimizing the Stack Configuration
119. nts per cycles are used in the calculation The cycles in the noise determination are not overlapping In order to obtain reliable results the lamp should be turned on for at least 10 minutes prior to measurement Test Name Quick Noise Test Description The test performs a quick Noise Evaluation without reference Module G1314E DE81960002 Status Passed Start Time 5 26 2010 12 34 42 PM Stop Time 5 26 2010 12 39 43 PM m Test Procedure Result k 4 A x 7 Name A Accumulated UY Lamp Burn Time 1389 39 h UY Lamp On Time 2 56 h Signal Noise value at 254 nm UV 0 003 m4U A 1 Check Prerequisites UW 2 Measure Noise Y 3 Evaluate Data Absorbance mAU 22 062 22 064 22 066 22 068 22 07 4 0 as 1 2 5 Time min Figure 43 Quick Noise Test with Agilent Lab Advisor Agilent 1200 Infinity Series VWD User Manual 123 8 Test Functions Dark Current Test Dark Current Test The dark current test measures the leakage current from the sample and reference circuits The test is used to check for defective sample or reference diodes or ADC circuits which may cause non linearity or excessive baseline noise During the test the shutter is moved into the light path Next the leakage current from both diodes is measured Test Name Dark Current Test Description The test measures the dark current from the detector optic Module G1314E DE819600
120. nts require that the detector should be turned on at least 24 hours before start of testing 32 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual e Installing the Detector J Unpacking the Detector 34 Damaged Packaging 34 Delivery Checklist 35 Detector Accessory Kit Contents 35 Optimizing the Stack Configuration 36 One Stack Configuration 37 Two Stack Configuration 41 Installation Information on Leak and Waste Handling 45 Installing the Detector 49 Flow Connections to the Detector 52 This chapter describes the installation of the detector ea Agilent Technologies 33 3 Installing the Detector Unpacking the Detector CAUTION Condensation within the detector Condensation will damage the system electronics gt Do not store ship or use your detector under conditions where temperature fluctuations could cause condensation within the detector gt If your detector was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agile
121. oltage in the above mentioned range Consequently there is no voltage selector in the rear of the detector There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Instrument is partially energized when switched off The power supply still uses some power even when the power switch on the front panel is turned OFF Repair work at the detector can lead to personal injuries e g shock hazard when the detector cover is opened and the instrument is connected to power gt To disconnect the detector from the power line unplug the power cord Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 20 Agilent 1200 Infinity Series VWD User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord
122. on and maintenance of the module Accessory Kit Accessory kit G1314 68755 contains some accessories and tools needed for installation and repair of the module p n 0100 1516 5062 8535 5063 6527 5181 1516 Description Fitting male PEEK 2 pk Waste accessory kit PEEK capillary 0 25 mm i d 1 16 o d 500 mm long plus 2 MT PTFE tubing i d 0 8 m 1 16 o d Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste CAN cable Agilent module to module 0 5 m Agilent 1200 Infinity Series VWD User Manual 151 10 Parts and Materials for Maintenance Standard Flow Cell 10 mm 14 pL Item p n Description G1314 60186 Standard flow cell 10 mm 14 pL 40 bar with RFID tag 5062 8522 Capillary column detector PEEK 600 mm lg 0 17 mm i d 1 16 inch o d G1314 65061 Cell Repair Kit includes 2x Gasket 1 2x Gasket 2 2x Window Quartz 1 G1314 65062 Cell screw kit 79853 29100 Conical spring kit 10 pk 3 G1314 65066 Ring 2 kit IN small hole i d 1 mm PEEK 2 pk 4 G1314 65064 Gaskets 2 IN small hole i d 1 mm KAPTON 10 pk 5 79853 68742 Window quartz kit 2 pk 6 G1314 65063 Gasket 1 kit OUT large hole i d 2 4 mm KAPTON 2 pk 7 G1314 65065 Ring 1 kit OUT large hole i d 2 4 mm PEEK 2 pk 8 G1314 44010 Clip for RFI ID tag 9 0515 4780 Screw for Clip M2 2 4 5 mm long 152 Agilent 1200 Infinity Series VWD User Manual Parts and Materials for Maintenance 10 Standard Flow Cell 10 mm 14 pL Y 1 Cell screw
123. on of BootP Service 211 Two Methods to Determine the MAC Address 213 Assigning IP Addresses Using the Agilent BootP Service 214 Changing the IP Address of an Instrument Using the Agilent BootP Service 217 Manual Configuration 219 With Telnet 220 With the Instant Pilot G4208A 224 PC and User Interface Software Setup 225 PC Setup for Local Configuration 225 User Interface Software Setup 226 This chapter provides information on connecting the detector to the Agilent ChemStation PC ote Agilent Technologies 197 13 LAN Configuration What you have to do first The module has an on board LAN communication interface 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear of the module underneath the configuration switch REMOTE 5 Cao SS CONFIG WAN RS 282 o LAN m LIL Joe G COMPACT FLASH 1314 65080 part number of board B 01 ZZ 0444 Revision Code Vendor Year and Week of assembly MAC 000001000001 MAC address Made in Germany Country of Origin Figure 60 Location of Configuration Switch and
124. or position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 232 Agilent 1200 Infinity Series VWD User Manual Appendix 14 UV Radiation Emissions of ultraviolet radiation 200 315 nm from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs Threshold Limit Values according to the American Conference of Governmental Industrial Hygienists Table 32 UV radiation limits Exposure day Effective irradiance 8h 0 1 uW cm 2 10 min 5 0 W cm Typically the radiation values are much smaller than these limits Table 33 UV radiation typical values Position Effective irradiance Lamp installed 50 cm distance average 0 016 W cm Lamp installed 50 cm distance maximum 0 14 pW cm 2 Agilent 1200 Infinity Series VWD User Manual 233 14 Appendix Solvent Information 234 Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Prevent any crystallization of buffer solutions This will lead into a blockage damage of the flow cell If the flow cell is transported while temperatures are below 5 C it must be assured that the cell is filled with alcohol Aqueous
125. ors Verification of wavelength accuracy with built in holmium oxide filter RFID for electronics records of flow cell and UV lamp conditions path length volume product number serial number test passed usage Housing All materials recyclable Agilent 1200 Infinity Series VWD User Manual 31 2 Site Requirements and Specifications Specification Conditions G1314F ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions Standard flow cell path length 10 mm flow 1 mL min LC grade methanol Noise 0 25 10 AU at 230 nm TC 2 s RT 2 2 TC Linearity Linearity is measured with caffeine at 265 nm The specification are based on the the standard RFID tag lamp G1314 60101 and may be not achieved when other lamp types or aged lamps are used ASTM drift tests require a temperature change below 2 C hour 3 6 F hour over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Performance tests should be done with a completely warmed up optical unit gt one hour ASTM measureme
126. ory of the module He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the module are not cleared automatically If not changed by the user they are still available when switching back to the mode Using Stored Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Dynamic Host Configuration Protocol DHCP General Information DHCP The Dynamic Host Configuration Protocol DHCP is an auto configuration protocol used on IP networks The DHCP functionality is available on all Agilent HPLC modules with on board LAN Interface or LAN Interface Card and B firmware B 06 40 or above When the initialization mode DHCP is selected the card tries to download the parameters from a DHCP Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d3177321 It is the DHCP server s responsibility to forward the hos
127. p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection RS 232 cables p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m Agilent 1200 Infinity Series VWD User Manual 165 11 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog an Center Center Analog 166 Agilent 1200 Infinity Series VWD User Manual Agilent Module to General Purpose Identifying Cables 11 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog S e Agilent 1200 Infinity Series VWD User Manual 167
128. ponse time for your detector The ane min i peak detector ignores any peaks that are inf gt 0 0025 min 0 06 s considerably narrower or wider than the mee peak width setting The response time is the time between 10 and 90 of the output 0 1 min 2 s Append signal in response to an input step function gt O 2min 4s Cut gt 0 4min 8s cut Copy Limits When you set the peak width in lt inutes the corresponding response time is _ gt Paste minu set automatically and the appropriate data rate for signal acquisition is selected as shown in Table 10 on page 78 Table 11 on Cancel Help More gt gt page 78 and Table 12 on page 79 Balance Scan Table Graphic Figure 33 Peakwidth Setting Agilent 1200 Infinity Series VWD User Manual 77 4 Using the Detector Table 10 Peak Width Response Time Data Rate G1314D peakwidth at half heigth Response Time s Data Rate Hz min lt 0 005 lt 0 12 20 gt 0 005 0 12 20 gt 0 01 0 25 20 gt 0 025 0 5 20 gt 0 05 1 0 10 gt 0 10 2 0 5 gt 0 20 4 0 2 5 gt 0 40 8 0 1 25 Table 11 Peak Width Response Time Data Rate G1314F peakwidth at half heigth Response Time s Data Rate Hz min lt 0 003125 lt 0 0625 80 gt 0 003125 0 0625 80 gt 0 00625 0 125 80 gt 0 0125 0 25 40 gt 0 025 0 5 20 gt 0 05 1 10 gt 0 1 2 5 gt 0 2 4 2 5 gt 0 4 8 1 25 78 Agilent 1200
129. position Ensure both cell holes can be seen through the holes of gasket The semi micro 1 and 2 gaskets items 6 and 7 Semi micro Flow Cell 6 mm 5 pL on page 156 look very similar Do not mix them up b Place the window on gasket c Place the peek ring on the window d Insert the conical springs Make sure the conical springs point towards the window Otherwise tightening the cell screw might break the window TI Ring Window Gasket Arrangement Figure 47 Orientation of conical springs e Screw the cell screw into the flow cell and tighten the screw Repeat the procedure for the other cell side Reconnect the capillaries Perform a leak test If OK insert the flow cell y Oo 1 A Perform Wavelength Verification Calibration on page 120 to check the correct positioning of the flow cell 8 Replace the front cover 142 Agilent 1200 Infinity Series VWD User Manual Maintenance and Repair 9 Using the Cuvette Holder This cuvette holder can be placed instead of a flow cell in the variable wavelength detector Standard cuvettes with standards in it for example National Institute of Standards amp Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications ff C2 f Li When If your own standard should be used to checkout the instrument Parts required p n Description 1 G1314 60200 Cuvette Holder 1 Cuvett
130. r current limit the error message is generated Probable cause Suggested actions 1 Lamp disconnected Ensure the lamp connector is seated firmly 2 Top foam removed while lamp is on Please contact your Agilent service representative 3 Defective or non Agilent lamp Exchange the lamp 4 Defective main board Please contact your Agilent service representative 5 Defective power supply Please contact your Agilent service representative 104 Agilent 1200 Infinity Series VWD User Manual UV lamp no voltage Error ID 7451 Error Information 7 The lamp anode voltage is missing The processor continually monitors the anode voltage across the lamp during operation If the anode voltage falls below the lower limit the error message is generated Probable cause 1 Defective or non Agilent lamp 2 Defective power supply 3 Defective main board Ignition Failed Error ID 7452 Suggested actions Exchange the lamp Please contact your Agilent service representative Please contact your Agilent service representative The lamp failed to ignite The processor monitors the lamp current during the ignition cycle If the lamp current does not rise above the lower limit within 2 5 s the error message is generated Probable cause 1 Lamp disconnected 2 Defective or non Agilent lamp 3 Defective power supply 4 Defective main board Agilent 1200 Infinity Series VWD User Manual Suggested actions Ensure the
131. rents of lamps that can be used for diagnosing baseline problems Refer to the Service Manual for details Agilent 1200 Infinity Series VWD User Manual Troubleshooting and Diagnostics 6 Status Indicators Two status indicators are located on the front of the detector The lower left indicates the power supply status the upper right indicates the detector status Status indicator green yellow red Dee 7 ia ay Ld r Line power switch with green light Figure 37 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Agilent 1200 Infinity Series VWD User Manual 91 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions 92 When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error
132. rmance Specifications G1314E 27 Performance Specifications G1314F 30 Installing the Detector 33 Unpacking the Detector 34 Optimizing the Stack Configuration 36 Installation Information on Leak and Waste Handling 45 Installing the Detector 49 Flow Connections to the Detector 52 Using the Detector 55 Leak and Waste Handling 56 Setting up an Analysis 57 Special Settings of the Detector 71 How to optimize the detector 83 Optimizing the Detector Performance 84 Match the Flow Cell to the Column 85 Set the Detector Parameters 88 Agilent 1200 Infinity Series VWD User Manual Contents Troubleshooting and Diagnostics 89 Overview of the Detector s Indicators and Test Functions 90 Status Indicators 91 Available Tests versus Interfaces 93 Agilent Lab Advisor Software 94 Error Information 95 What Are Error Messages 97 General Error Messages 98 Detector Error Messages 104 Test Functions 115 Intensity Test 116 Cell Test 118 Wavelength Verification Calibration 120 ASTM Drift and Noise Test 122 Quick Noise Test 123 Dark Current Test 124 Holmium Oxide Test 126 Maintenance and Repair 129 Introduction to Maintenance 130 Warnings and Cautions 131 Overview of Maintenance 133 Cleaning the Module 134 ExchangingaLamp 135 Exchanging a Flow Cell 138 Repairing the Flow Cells 140 Using the Cuvette Holder 143 Correcting Leaks 145 Replacing Leak Handling System Parts 146 Replacing the Module s Firmware 147 A
133. roubleshooting and diagnostic features Apg Agilent Technologies 89 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 90 Status Indicators The detector is provided with two status indicators which indicate the operational state prerun run and error states of the detector The status indicators provide a quick visual check of the operation of the detector Status Indicators on page 91 Error Messages In the event of an electronic mechanical or hydraulic failure the detector generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided by the user interface Refer to the Service Manual for details Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components Refer to the User Interface and or the Service Manual for details Wavelength Verification Recalibration Wavelength recalibration is recommended after repair of internal components and on a regular basis to ensure correct operation of the detector The detector uses the deuterium alpha and beta emission lines for wavelength calibration see Wavelength Verification Calibration on page 120 Diagnostic Signals The detector has several signals internal temperatures voltages and cur
134. rposes Activity Solvent Comments After an installation lsopropanol Best solvent to flush air out of the system When switching between reverse lsopropanol phase and normal phase both times Best solvent to flush air out of the system After an installation Ethanol or Methanol Alternative to lsopropanol second choice if no lsopropanol is available To clean the system when using Bidistilled water Best solvent to re dissolve buffer buffers crystals Bidistilled water After a solvent change Best solvent to re dissolve buffer crystals After the installation of normal phase Hexane 5 lsopropanol Good wetting properties seals P N 0905 1420 The pump should never be used for priming empty tubings never let the pump run dry Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump 1 Open the purge valve of your pump by turning it counterclockwise and set flow rate to 3 5 mL min 2 Flush all tubes with at least 30 mL of solvent Set flow to required value of your application and close the purge valve Pump for approximately 10 minutes before starting your application 58 Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 Requirements and Conditions What You Will Need The table below lists the items you need to have for the set up of the analysis Some of these are optional not required for the basic system Tabl
135. s Interfaces Interface Test Agilent Lab Advisor Agilent ChemStation Instant Pilot G4208A Wavelength yes Tests Maintenance Verification Re calibration Lamp Intensity yes Tests Diagnosis Holmium Test yes Tests Diagnosis Cell Test yes Tests n a D A Converter Test yes Tests n a Filter Grating Motor Test yes Tests command line Test Chromatogram yes command line command line Spectrum Blank Sample Holmium yes Service Dialog for service only n a n a Control for service only interface provides passed fail information or a plot requires a comand via comand line requires a comand via comand line in Service Mode Agilent 1200 Infinity Series VWD User Manual 93 Troubleshooting and Diagnostics Agilent Lab Advisor Software 94 The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor t
136. s about 2 5 min and is disabled within the first 10 min after ignition of the lamp because initial drift may distort the measurement When the lamp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically The Wavelength Verification Calibration is available in e Agilent Lab Advisor preferred tool e Agilent Instant Pilot G4208A via More Diagnosis VWD Calibration When to Calibrate the Detector The detector is calibrated at the factory and under normal operating conditions should not require recalibration However it is advisable to recalibrate e after maintenance flow cell or lamp e after repair of components in the optical unit e after exchange of the optical unit or VWM board e at a regular interval at least once per year for example prior to an Operational Qualification Performance Verification procedure and e when chromatographic results indicate the detector may require recalibration Agilent 1200 Infinity Series VWD User Manual Test Functions 8 Wavelength Verification Calibration Wavelength Verification Calibration with Agilent Lab Advisor Test Name Wavelength Calibration Description This procedure performs a Wavelength Verification and Recalibration Module G1314E DE81960002 Approx Time 3 min Status Running IIT IIT TTT TT r Test Procedure m Result Name Value W 1 Check Prerequisites Accumulated UV Lamp Bum Time 1389 70 h A 2
137. s located in C Documents and Settings All Users Application Data Agilent BootP LogFile It contains a MAC address entry for each device that requests configuration information from BootP 3 Click OK to save the values or Cancel to discard them The editing ends 4 After each modification of the BootP settings i e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 217 or Restarting the Agilent BootP Service on page 218 5 Uncheck the Do you want to log BootP requests box after configuring instruments otherwise the log file will quickly fill up disk space Determining the MAC address directly from the LAN Interface card label 1 Turn off the instrument 2 Read the MAC address from the label and record it The MAC address is printed on a label on the rear of the module It is the number below the barcode and after the colon and usually begins with the letters AD see Figure 60 on page 198 3 Turn on the instrument Agilent 1200 Infinity Series VWD User Manual 213 13 LAN Configuration Assigning IP Addresses Using the Agilent BootP Service The Agilent BootP Service assigns the Hardware MAC address of the instrument to an IP address Determining the MAC address of the instrument using BootP Service 1 Power cycle the Instrument 2 After the instrument completes self test open the log file of the BootP
138. s that are divided into common functions like e run synchronization through APG remote error handling diagnostic functions e or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 176 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Firmware Updates Firmware updates can be done using your user interface PC and Firmware Update Tool with local files on the hard disk e Instant Pilot G4208A with files from a USB Flash Disk Agilent Lab Advisor software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 56 Firmware Update Mechanism Agilent 1200 Infinity Series VWD User Manual 177 12 Hardware Information 178 Some mod
139. should carry an Agilent 1200 Infinity Series system make sure that the bench is designed to bear the weight of all modules The detector should be operated in a horizontal position Environment CAUTION Your detector will work within specifications at ambient temperatures and relative humidity as described in Physical Specifications on page 23 Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 22 Agilent 1200 Infinity Series VWD User Manual Physical Specifications Site Requirements and Specifications 2 Table 2 Physical Specifications Type Specification Comments Weight 11 kg 25 Ibs Dimensions 140 x 345 x 435 mm height x width x depth 5 5 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 85 W 290 BTU Maximum Ambient operating temp
140. sident System and Revert to Default Data Coldstart refer to Special Settings on page 191 190 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 22 Boot Resident Settings On board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 sws TEST BOOT 1 1 1 0 0 0 0 0 Agilent 1200 Infinity Series VWD User Manual 191 12 Hardware Information Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start
141. sis high or low sensitivity analysis wavelength etc therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary take note of the values displayed by lamp counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled This function is only available via Agilent Lab Advisor or Instant Pilot Agilent 1200 Infinity Series VWD User Manual 195 12 Hardware Information Early Maintenance Feedback EMF 196 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series VWD User Manual 13 LAN Configuration What you have to do first 198 TCP IP parameter configuration 199 Configuration Switches 200 Initialization mode selection 201 Dynamic Host Configuration Protocol DHCP 205 General Information DHCP 205 Setup DHCP 206 Link configuration selection 208 Automatic configuration with Bootp 209 About Agilent BootP Service 209 How BootP Service Works 210 Situation Cannot Establish LAN Communication 210 Installati
142. solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents for example acetonitrile or methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents e Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on e High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly O gt gt 2COCl 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Agilent 1200 Infinity Series VWD User Manual Appendix 14 Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions conta
143. t off filter in light path at A gt 370 nm HOLMIU holmium oxide filter for wavelength check M SHUTTER for measurement of dark current of photo diodes A photo sensor determines the correct position 14 Agilent 1200 Infinity Series VWD User Manual Introduction to the Variable Wavelength Detector 1 Mirror Assemblies M1 and M2 The instrument contains two spherical mirrors M1 and M2 The beam adjustable is vertically and horizontally Both mirrors are identical Grating Assembly The grating separates the light beam into all its component wavelengths and reflects the light onto mirror 2 The stepper motor reference position is determined by a plate fitted onto the motor shaft interrupting the beam of a photo sensor The wavelength calibration of the grating is done at the zero order light position and at 656 nm which is the emission line of the deuterium lamp Beam Splitter Assembly The beam splitter splits the light beam One part goes directly to the sample diode The other part of the light beam goes to the reference diode Photo Diodes Assemblies Two photo diode assemblies are installed in the optical unit The sample diode assembly is located on the left side of the optical unit The reference diode assembly is located in the front of the optical unit Photo Diode ADC analog to digital converter The photo diode current is directly converted to digital data direct photo current digitalization The data is tra
144. te safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 1200 Infinity Series VWD User Manual Appendix 14 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1200 Infinity Series VWD User Manual 231 14 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operat
145. tector Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 Optimization of the System The settings used for this analysis are specific for this purpose For other applications the system can be optimized in various ways Please refer to the section Optimizing the Detector Performance on page 84 Preparing the HPLC System 1 Turn on the Agilent ChemStation PC and the monitor 2 Turn on the modules 3 Start the Agilent ChemStation software If the pump autosampler thermostatted column compartment and detector are found the Agilent ChemStation screen should look like shown in Figure 17 on page 61 The System status is red Not Ready System status Method and Run Control Last Run es Method DEF_LcM Gj Sequence DEF Lcs EM smn SO 0 bar gt 0 000 ml min I oe en ATE O DEFAULT D ea eal no Details E data E 1 1 GB free Online plot window details window Figure 17 Initial Agilent ChemStation screen Method and Run Control Agilent 1200 Infinity Series VWD User Manual 61 4 Using the Detector 4 Turn on the detector lamp pump and autosampler by clicking the System On button or the buttons below the module icons on the graphical user interface GUI After some time the pump thermostatted column compartment and detector module will turn to green Method and Run Control ee E 002 ccc ters EME
146. tep gradients the micro flow cell 2 uL 3 mm gives the best performance e In high resolution analysis time is not the highest priority Higher delay volumes are accepted Therefore we recommend to use the damper plus mixer for a highest signal to noise If longer columns gt 50 mm for higher resolution are used then the next larger flow cell is the preferred choice for higher sensitivity Agilent 1200 Infinity Series VWD User Manual 85 5 86 How to optimize the detector Flow Cell Path Length Lambert Beer s law shows a linear relationship between the flow cell path length and absorbance Absorbance log T log exCxd where T is the transmission defined as the quotient of the intensity of the transmitted light divided by the intensity of the incident light Ip e is the extinction coefficient which is a characteristic of a given substance under a precisely defined set of conditions of wavelength solvent temperature and other parameters C is the concentration of the absorbing species mol L d m is the path length of the cell used for the measurement Therefore flow cells with longer path lengths yield higher signals Although noise usually increases little with increasing path length there is a gain in the signal to noise ratio For example in Figure 36 on page 87 the noise increased by less than 10 but a 70 increase in signal intensity was achieved by increasing the path length
147. ter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return Agilent 1200 Infinity Series VWD User Manual 221 13 LAN Configuration 5 Use the and press Enter to list the current settings cx Telnet 134 40 27 104 Agilent Technologies G1314E DE81966062 gt 4 LAN Status Page MAC Address information about the LAN interface 6636D30611BD MAC address initialization mode Initialization mode is Using Stored IP Address 134 40 27 104 Subnet Mask 255 255 248 0 active TCP IP settings Def Gateway 134 48 24 1 TCP IP Status TCP IP status here ready no connections connected to PC with controller software gt ip 134 40 27 99 gt LAN Status Page here not connected Figure 77 Telnet Current settings in Using Stored mode 6 Change the IP address in this example 134 40 27 99 and type to list current settings change of IP setting to Initialization mode is Using Stored TCP IP Properties active IP Address Subnet Mask Def Gateway stored IP Address Gateway 222 134 40 27 104 255 255 248 0 i i 134 40 34 1 active TCP IP settings 134 48 27 99 o 255 255 248 0 stored TCP IP settings in non volatile memory 134 490 24 1 TCP IP status here ready no connact zons connected to PC with controller software here not connected Figure 78 Telnet Change IP set
148. the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1200 Infinity Series VWD User Manual In This Guide In This Guide This manual covers e the Agilent 1290 Infinity Variable Wavelength Detector G1314E e the Agilent 1260 Infinity Variable Wavelength Detector G1314F and e the Agilent 1200 series Variable Wavelength Detector G1314D obsolete Find information on other Agilent Variable Wavelength Detectors in separate manuals 1 Introduction to the Variable Wavelength Detector This chapter gives an introduction to the detector instrument overview and internal connectors 2 Site Requirements and Specifications This chapter gives information on environmental requirements physical and performance specifications 3 Installing the Detector This chapter describes the installation of the detector 4 Using the Detector This chapter provides information on how to set up the detector for an analysis and explains the basic settings 5 How to optimize the detector This chapter gives hints on how to select the detector
149. tings Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 7 When you have finished typing the configuration parameters type exit and press Enter to exit with storing parameters cx C WINDOWS system32 cmd exe Agilent Technologies G1314E DE81966002 gt exit Connection to host lost GsN gt Figure 79 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted Agilent 1200 Infinity Series VWD User Manual 223 13 LAN Configuration With the Instant Pilot G4208A To configure the TCP IP parameters before connecting the detector to the network the Instant Pilot G4208A can be used 1 From the Welcome screen press the More button 2 Select Configure 3 Press the VWD button 4 Scroll down to the LAN settings Q Configure VWD SL Stays off at power on Temperature Control ON UV Lamp Type Auto detect from RFID tag info Cell Tag Use only cells with RFID tag LAN Subnet Mask 255 255 248 0 LAN Def Gateway 134 40 24 1 System I Controller VWD SL I Instant Pilot LAN Configuration Figure 80 5 Press the Edit button only visible if not in Edit mode perform the required changes and press the Done button 6 Leave the screen by clicking Exit 224 Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13
150. tname address information to the Domain Name Server The card does not offer any services for hostname resolution e g NetBIOS DHCP Active Server Parameter Figure 66 DHCP Principle 1 It may take some time until the DHCP server has updated the DNS server with the hostname information 2 It may be necessary to fully qualify the hostname with the DNS suffix e g 0030d3177321 country company com 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions Agilent 1200 Infinity Series VWD User Manual 205 13 LAN Configuration Setup DHCP Software required LONTIQ ation Frotocol L The modules in the stack must have at least firmware from set A 06 34 and the above mentioned modules B 06 40 or above must from the same firmware set 1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Instant Pilot the MAC address can be found under Details in the LAN section System Info Controller DE12345678 G4208A Main Revision DAD EX RPA Cn 64260019 G1315D Main Revision _ Resident Revison B 06 41 0002 B 06 40 0007 On time 3d 01 33h Installed Options Dhcp LAN TCP IP Mode DHCP LAN TCP IP Address 130 168 132 219 0030D314F89E TYPE G61315 66565
151. to Link configuration selection on page 208 The detector was shipped with default configuration settings all switches down Place the detector in the stack or on the bench in a horizontal position 4 Ensure the line power switch at the front of the detector is OFF Connect the power cable to the power connector at the rear of the detector 6 Connect the CAN cable to other modules 50 Agilent 1200 Infinity Series VWD User Manual Installing the Detector 3 7 Connect the LAN cable e g from a Agilent ChemStation as controller to the detector s LAN connector In multi detector configurations the LAN of the Agilent detector with the highest data rate must be used due to its higher data load 8 Connect the analog cable s optional 9 Connect the APG remote cable optional for non Agilent 1200 Infinity Series instruments 10 Turn on power by pushing the button at the lower left hand side of the detector The status LED should be green Status indicator green yellow red E r ine te i i 4 with green light The detector is turned on when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned off when the line power switch is protruding and the green light is off To disconnect the detector from line unplug the power cord The power supply still uses some power even if the power switch at the front panel is turned off A
152. to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Open Cover Error ID 0205 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service representative operation 2 Foam not activating the sensor Please contact your Agilent service representative 3 Defective sensor or main board Please contact your Agilent service representative Agilent 1200 Infinity Series VWD User Manual 103 7 Error Information Detector Error Messages These errors are detector specific UV lamp no current Error ID 7450 The lamp anode current is missing The processor continually monitors the anode current drawn by the lamp during operation If the anode current falls below the lowe
153. tribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 188 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Setting the 8 bit Configuration Switch The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e Default is ALL switches DOWN b
154. tuation of the light source A slit in front of the reference photodiode cuts out light of the sample bandwidth Wavelength selection is made by rotating the grating which is driven directly by a stepper motor This configuration allows fast change of the wavelength The cutoff filter is moved into the lightpath above 370 nm to reduce higher order light Deuterium lamp a i Filter assembly Entrance slit Mirror M1 Lens Sample diode f Flow cell Grating Mirror M2 Beam splitter Reference diode Figure 1 Optical Path of the Variable Wavelength Detector Agilent 1200 Infinity Series VWD User Manual 11 1 Introduction to the Variable Wavelength Detector Flow Cell A variety of flow cell cartridges can be inserted using the same quick and simple mounting system The flow cells have an integrated RFID tag that contains the flow cell specific information e g part number cell volume path length A RFID tag reader reads out this information and transfers it to the user interface RFID tag S Figure 2 Flow Cell with RFID tag Table1 Flow Cell Data STD Semi micro Micro High Pressure Maximum pressure 40 4 40 4 120 12 400 40 bar Path length 10 conical 6 conical 3 conical 10 conical mm Volume 14 5 2 14 uL Inlet i d 0 25 0 17 0 12 0 25 mm Inlet length 750 250 310 750 mm Outlet i d 0 30 0 17 0 17 0 17 mm Outlet length 120 120 120 120 mm Total volume 60 77 14 49 14 00 60
155. ucts only in the manner described in the Agilent product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 132 Agilent 1200 Infinity Series VWD User Manual Overview of Maintenance Maintenance and Repair 9 The following pages describe maintenance simple repairs of the detector that can be carried out without opening the main cover Table 16 Simple Repairs Procedures Typical Frequency Notes Deuterium lamp If noise and or drift exceeds your application limits or A VWD test should be performed after exchange lamp does not ignite replacement Flow cell exchange If application requires a different flow cell type A VWD test should be performed after replacement Cleaning flow cell If leaking or if intensity drops due to contaminated flow A pressure tightness test should be parts cleaning or cell windows done after repair exchange Leak sensor drying If leak has occurred Check for leaks Leak handling system If broken or corroded replacement Check for leaks Agilent 1200 Infinity Series VWD User Manual 133 9 Maintenance and Repair Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARN
156. ules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 Agilent 1200 Infinity Series VWD User Manual Hardware Information 12 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection T
157. umentation from Agilent web site Read update documentation provided with the Firmware Update Tool 9 To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Agilent 1200 Infinity Series VWD User Manual 147 9 Maintenance and Repair Module Specific Information Table 17 Module Specific Information G1314D G1314E G1314F Initial firmware B 06 20 B 06 20 B 06 30 Compatibility with When using the G1314D ina When using the G1314E ina When using the G1314F in a 1100 1200 series system all other modules system all other modules system all other modules modules must have firmware revision must have firmware revision must have firmware revision A 06 10 or B 06 10 or above A 06 10 or B 06 10 or above A 06 30 or B 06 30 or above main and resident Otherwise main and resident Otherwise main and resident Otherwise the communication will not the communication will not the communication will not work work work Conversion to Not possible due to different emulation of G1314B hardware and electronic or G1314C platform 148 Agilent 1200 Infinity Series VWD User Manual Agilent 1200 Infinity Series V
158. ure the Run Recovery dialog box allows you to restore the stored data to the data directory From there you can copy the files to the directory where the files are corrupted or not complete On very large recovery files it may take a long time to restore it to the Agilent ChemStation A sequence will be stopped in case of a network problem When during recovery an error Method Sequence stopped appears the instrument logbook shows an entry No Run data available in device In this case refer to No Run Data Available In Device on page 112 Agilent 1200 Infinity Series VWD User Manual Using the Detector 4 Automated Run Recovery in case of temporary communication failures Table 13 Automated Run Recovery in case of temporary communication failures Situation Reaction On ChemStation All OK Run in progress Data Analysis vWD Status Run Rawdata Rawdata Elapsed run time is running Data are stored on PC and on card LAN breaks e Run in progress Data Analysis vw Status Run Rawdata Fawdata EOI LAN recovers Stop time elapsed Run ends Error Power Fail Wavelength 254 nm Elapsed run time stops Data continues to be stored on card Run in progress Data Analysis Run Rawdata Rada Error Power Fail cleared e Sim Elapsed run time continues at actual time Spectra counter continues Data continues to be stored on PC and on card ChemStation tries already to add missing data
159. us Passed Start Time 5 26 2010 12 31 17 PM Stop Time 5 26 2010 12 33 17 PM m Test Procedure Result A 1 Check Prerequisites Accumulated UV Lamp Bum Time 1389 33 h AW 2 Perform Intensity Test UV Lamp On Time 3 Evaluate Data Lowest Intensity 22 970 Counts 735 739 Cans Highest Intensity 5 211 248 Counts Intensity Counts 5 2112E 06 4E 06 2E 06 22970 4 1 500 Wavelength nm Figure 38 Intensity Test with Agilent Lab Advisor Intensity Test Failed Probable cause Suggested actions 1 Empty flow cell Ensure the flow cell is filled with water 2 Flow cell windows dirty Repeat the test with the flow cell removed If the test passes exchange the flow cell windows 3 Optics defect Please contact your Agilent service representative 4 Defective lamp or optics Exchange the lamp Agilent 1200 Infinity Series VWD User Manual 117 8 Test Functions Cell Test 118 The cell test compares the intensity of the deuterium lamp measured by the sample and reference diodes unfiltered and not logarithmized when the grating is in the zero order position The resulting intensity ratio sample reference is a measure of the amount of light absorbed by the flow cell The test can be used to check for dirty or contaminated flow cell windows When the test is started the gain is set to 1 To eliminate effects due to absorbing solvents the test should be done with water in the flow cell
160. vailable Signals Selected Signals 60 2 40 uaternary Pump Flow 30 aternary Pump oe 204 uaternary Pump B E Adata 4104 uaternary Pump C 4 waternary Pump D E E 1 1 GB free column Thermostat Temperature l column Thermostat Temperature ri WD B Signal w o Reference at 254 Y gt Window _ WWD amp Absorbance at 254 nm gt x axis 15 Hmn Type acquired y axis range 1 mau T draw zero line T auto y adjust Offset 10 m Method Settings J Use method settings Apply to Method OK Cancel Help Figure 23 Edit Signal Plot Window Agilent 1200 Infinity Series VWD User Manual 67 4 Using the Detector Setting up an Analysis The Online Plot Figure 24 on page 68 shows both the pump pressure and the detector absorbance signals Pressing Adjust the signals can be reset to the offset value and Balance would do a balance on the detector i i Online Plot Quaternary Pump Pressure Figure 24 Online Plot Window 13 If both baselines are stable set the Y range for the detector signal to 100 mAU If you start with a new UV lamp for the first time the lamp may show initial drift for some NOTE j time burn in effect 68 Agilent 1200 Infinity Series VWD User Manual 14 Select the menu item RunControl gt Sample Info and enter information about this application Figure 25 on page 69 Press OK to leave this screen Offline D
161. y time it is powered on the recommended initialization mode is Bootp Agilent 1200 Infinity Series VWD User Manual LAN Configuration 13 Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Non Volatile RAM Active Parameter Stored Parameter Figure 64 Using Stored Principle Using Default When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 26 on page 204 Default Active Parameter Parameter Figure 65 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immediately Agilent 1200 Infinity Series VWD User Manual 203 13 LAN Configuration 204 Table 26 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the module must reside in the same subnet The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile mem

1200 Infinity Series Variable Wavelength Detectors User Manual

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