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FORCED SWIM SYSTEM - Med Associates Inc.

1. F gt VV iV I LD FA IOUT AT CO I INN UNCLE LY J VV i Running a Component Analysis To run a component analysis click Component Analysis Run Component Analysis The Component Summary Report screen shown in Figure 5 4 will appear Click on the button next to Component File and browse to the desired cmp file The click on the w button next to Output File select the desired output location and enter an Output File Name Next enter the Upper and Lower Threshold that should be used to calculate the Above Between and Below Threshold Counts and Times Click OK and the Select file s to report on screen shown in Figure 5 5 will appear Figure 5 4 Component Summary Report Screen Component Summary Report Component File Output File E Browse to cmp File Enter csv File Name and Location Enter Upper amp Lower Thresholds to Upper Threshold be used to calculate Counts and Times Lower Threshold J cms Select the fsa file s to run the component analysis on and click OK The screen shown in Figure 5 6 will appear Figure 5 5 Select File s to Report On Select file s to report on Look in Forced Swim 082508 ha m Forced Swim 082508 Rat 010 fsa EF m Forced Swim 082608 Rat 014 analyzed fsa My Recent Forced Swim 082608 Rat 014 fsa Documents m Forced Swim test Mouse HO fsa m Forced Swim test Mouse H1 fsa Forced Swim test Mouse H2 fsa m Forced Swim test Mou
2. Range Cursors To obtain data from a specified interva of time within the session use the Range Cursors Enable the Range Cursors by selecting Graph Show Range Cursors The blue range cursors will appear on the Motion Index Graph and the position of the cursors as well as the time difference Delta between the cursors will appear above the Experiment Data as shown below Adjust the position and length of the specified interval by clicking and dragging the cursors to the desired position in the Motion Index Graph or by entering the desired positions in the Range Cursors 1 and 2 fields Adjusting the position of the Range Cursors will alter the data shown in the Experiment Data portion of the Main Screen Figure 3 1 and Figure 3 10 The figure below shows the Range cursors Note that when the Range Cursors are shown the Experiment Data reflect only the data that exist between the Range Cursors on the Motion Index Graph Figure 3 12 Range Cursors Forced Swim C Documents and Settings Forced SwimWesktop Forced Swim 082508 Forced Swim 082608 Rat 01 E File Graph Zoom Component Analysis Help E Experiment Subject Group Trial Total Time s 300 033 Thresholds Lower BGT upper Range Cursors 1 READER gt Delta 60 007 Time s _ Samples Sum Above Upper Threshold 5 467 164 1517171 Between Thresholds 49 033 1471 7900902 Below Lower Threshold 5 933 166 472727 I i i i 66 66 99 99 133 32 166 65
3. File oooccccccncccncccnnnnnnnnnr anno rr 18 ZOOMING IN on Te Motion Index Crasas 19 APP elo oO 20 Saving a Motion Data fSa File oooooccccccccccnnnnncnana nano 20 CHApterS ansia 21 Component AN GIY SS roscar erat 21 AANO COMPONEN aca is tbaneeen ic agianes N 21 AA ee O IA 22 Deleting COMPONENTS 00cccccccncccncccnnnnnnnnc raro 22 Savilid a Component ANAIYSIS urna eo 22 Loading a COMPOnenitAnalVS Siesta arista 22 Running a Component ANALYSIS 0 ooocccccccccccnnnncnnncna no nan nnnr ran 23 Example EV a ee A EN E ee eer ee eee ee 24 APPEND A ains 25 CHAPTER 1 Introduction The Forced Swim Test is a standard means of testing the pharmacological effects of antidepressants in mice and rats Dose dependent effects on immobility are evident using the Forced Swim Test and the Med Associates software provides researchers with a reliable method of behavioral quantification The MED Associates Forced Swim System includes everything needed to conduct this behavioral assay in rodent species The primary dependent variable is the amount of time spent immobile during the experimental session The software provides an index of average motion and data are displayed in real time The MED Associates Forced Swim System includes the Video Monitor Plug In for Forced Swim as well as the Forced Swim hardware The Video Monitor Plug In for Forced Swim allows the researcher to analyze video recorded using the MED Associates Video M
4. be advanced or reversed by clicking and dragging the Time Cursor to the desired location on the Motion Index Graph Figure 3 8 Thresholds and Video Location Indicator Labeled Time Cursor Upper Threshold Lower Threshold Figure 3 9 Time Cursor and Time s Correspond A Te 174 29 seconds Experiment Data The Experiment Data portion of the Main Screen shown below displays the data that corresponds to the position of the Upper and Lower Thresholds The Time s column contains the amount of time in seconds that the motion index was above the Upper Threshold between the Upper and Lower thresholds and below the Lower threshold The Samples column contains the number of frames that the motion index was above the Upper Threshold between the Upper and Lower Thresholds and below the Lower Threshold The Sum column contains the sum of the data above the Upper Threshold between the Upper and Lower Thresholds and below the Lower Threshold Figure 3 10 Experiment Data Time 5 samples Sum Above Upper Threshold NN Between Thresholds Below Lower Threshold 13 Jd Experiment Information The Experiment Information portion of the Main Screen shown below allows the user to enter useful information relating to the video file that will be saved with the analysis Figure 3 11 Experiment Information Experiment Forced Swim Experiment 1 Subject Subject 1 Group Group 1 Trial Testing 1
5. the NIR 100 Light Controller to the connector on the bottom of the NIR 100R Light Panel Figure 2 15 Connect the Light Panel to the Light Controller 6 6 6 6 ENON m ANAN senom soon g ane AZAZ SZ SZ Az gt eed Back of NIR 100 EH EH O O Light Controller Control tapi Control iSput To Light Panel 3 Using the power adapter connect the NIR 100 Light Controller to a GFCI outlet Figure 2 16 Connect Light Controller to GFC Outlet Back of NIR 100 Light Controller GFCI Wall Outlet mH mum de MIR Light White Light ra aad Control Input Control Input To Light Panel CHAPTER 3 User Interface This chapter describes the menus and features of the Forced Swim software application Open the Forced Swim software application and the main screen shown below will appear Figure 3 1 Main Screen Labeled Menu Bar Forced Swim Experiment Information Experiment Duration Video Display amp Threshold Locations Tre 5 Samplos Suen f ol of r Experiment Data Video Playback E E Controls Batanon Trecsholis 0 Tr o 0 ES l Below Lower Threshoid 0 Motion Index Graph Menu Bar File Menu Figure 3 2 File Menu Ese Zoom Component Analysis Help Save s Create Motion Data Exit Menu I tem Description For More I nfo Chapter 4 Opening a Motion Data Open Open fsa file fsa File Close Close fsa file N A Save the fsa file using the same Chapter 4 Saving a
6. 199 98 233 31 266 64 299 97 MED ASSOCIATES INC FORCED SWIM Video Display and Playback Controls The Video Display and Playback Controls portion of the main screen is shown below The Jump to Beginning Rewind Fast Forward and Jump to End buttons will function only when the video is Paused If the video is paused the video can be advanced or reversed by clicking and dragging the Time Cursor to the desired location on the Motion Index Graph see the Upper and Lower Thresholds and Time Cursor section of this manual Figure 3 13 Video Display and Playback Controls Video Display Current Place in Video Jump to Beginning Jump to End Play Pause Fast Forward TE CHAPTER 4 Using the Software Creating a Motion Data File The process of creating a motion data file is essentially extracting Motion Index data from a video file The motion data file will be saved as an fsa file and can be opened using the Video Monitor Plug In for Forced Swim To create a motion data file open the software application and the Main Screen Figure 3 1 will appear Select File Create Motion Data and the Open screen shown in Figure 4 1 will appear Figure 4 1 Select wmv File Look in E Forced Swim 082508 a et Eg E Forced Swim 062508 Rat O10 E Forced Swim test Mouse H1 wmv E Forced Swim 082508 Rat 011 wmw E Forced Swim test Mouse H2 wm My Recent E Forced Swim 082508 Rat 012 wmv E Forced Swim t
7. 46 68 6 2 51 53 2 27 Forced Swim Test Rati Group 1 Testing 1 3 179 1481 140 5 97 49 37 4 67 Forced Swim Test Rat1 Group 1 Testing 1 4 178 1587 35 5 93 52 9 1 17 Forced Swim Test Rat1 Group 1 Testing 1 5 432 1278 90 14 4 426 3 Forced Swim Test Rat1 Group 1 Testing 1 Total 1046 7357 597 34 87 245 23 19 9 MED ASSOCIATES INC FORCED SWIM APPENDIX A Software Installation Insert the Forced Swim CD into the CD ROM drive and the screen shown in Figure A 1 will appear Figure A 1 Forced Swim Main Screen A Forced Swim med ASSOCIATES ING Welcome to Forced Swim Install Forced Swim Extras Browse the CD Click Install Forced Swim and the screen shown in Figure A 2 will appear Figure A 2 Click Install to Begin Forced Swim Install Wizard med ASSOCIATES ME Welcome to the install wizard for Forced Swim The wizard will install Forced Swim on your computer To continue click Install Forced Swim Cancel 25 The Customer Information screen shown below will appear next Enter the desired User Name and Company Name as well as the Password that was issued by MED Associates when the software was registered Click Next to continue Figure A 3 Customer Information Screen Forced Swim InstallShield Wizard Customer Information Please enter your information Please enter your name the name of the company for which you work and the product serial number User Name User Mame Com
8. FORCED SWIM SYSTEM USER S MANUAL SOF 842 3 and Forced Swim Hardware User s Manual DOC 216 Rev 1 0 Copyright 2008 All Rights Reserved MED Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com MED ASSOCIATES INC FORCED SWIM TABLE OF CONTENTS Chapter L iia darian 1 Ji cunts tesa av A E A E eraser astenteaacey an 1 General Computer Env iROmMVeliilss secsscesecenecureeesceuscuesess sectannaseustensnseeurdiens reiini 1 CRADECD Zoraida 2 PASSO INSIrUCHONS rra 2 rar CO e A 2 Mia AA PPP e El o An 4 Installing the Camera Molero 5 Hardware ASSIM Oy cs ca in ias 6 Adjusting the Camera Focus and Aperture occcccccnncccononcnncnonnnnnanonrnna rr rra rare r ranas 7 Wiring INSTRUCTIONS nasorrrairc nietas telde rara 8 CRADLE Sian o 9 Boce aE e E E E E A tc 9 MENU Baca os 9 PING ICING earar ro T AE ose 9 Sra Mr 10 ZOOM Men Usain oia opi adobo iniciados 11 Component Analysis Men sec roscemestco robaron revistan ento 11 o g sear sess ctr A o O 12 MOON MIS ODA seras cas rocas 13 Upper and Lower Thresholds and Time Cursor occccccccnnonccnnccanonnnnnnna ran rra 13 AMALIA CD 1 PPP o o 5 yUue e ono 14 EXPern mento Mad scared abel 14 ad ALO GC C SONG MU II set E E AE 15 Video Display and Playback CONtrolS oooccccccccccnnnccconnnccononnnncno nr nnra nor rnrr anno 16 Ehapterd4 naaa iia 17 USINO ENS SOI usd o da a da 17 Creating a Motion Data Pluna rice 17 Opening a Motion Data fSa
9. Motion Data eau File Name and Location fsa File Menu I tem Description For More I nfo Save the fsa file using a different File Name and or Location Chapter 4 Saving a Motion Data A paves fsa File Create Motion Data Create an fsa file from a wmv file Chapter 4 Creating a Motion Data File Exit Close the software application N A Graph Menu Figure 3 3 Graph Menu File zoom Component Analysis Help Time Cursor to Center Thresholds to Center Show Range Cursors Range Cursors to Center Move Cursors Pan Y Pan Pan Y Menu Item Time Cursor to Center Thresholds to Center Show Range Cursors Range Cursors to Center Move Cursors Pan XY Pan X Pan Y Ctrl C Ctrl F Description Moves the Time Cursor to the center of the Motion Index Graph Moves the Threshold Cursors to the center of the Motion Index Graph Displays the Range Cursors on the Motion Index Graph Moves the Range Cursors to the center of the Motion Index Graph Mouse pointer will move cursors only Mouse pointer will pan the motion index graph and cursors along both the X and Y axes Mouse pointer will pan the motion index graph and cursors along the X axis only Mouse pointer will pan the motion index graph and cursors along the Y axis only For More Info Chapter 3 Upper and Lower Thresholds and Time Cursor Chapter 3 Upper and Lower Thresholds and Time Cursor Chapter 3 Upper and Lower Thresh
10. dd New to add the new component and keep the Component screen open Once the Component screen is closed the Component Setup screen will display any components that have been added An example Setup Component screen with several components added is shown in Figure 5 3 Figure 5 3 Example Component Setup Screen Setup Component test cmp Component Mame Start Time 00 00 00 000 560 000 00 01 00 000 60 000 00 02 00 000 60 000 00 03 00 000 60 000 00 04 00 000 60 000 00 00 00 000 300 000 Save Os Editing Components To edit the Name Start Time or Duration of a component highlight the desire component and click Edit The Component screen shown in Figure 5 2 will appear Make the necessary changes and click OK Deleting Components Highlight the desired component and click Delete and the component will be removed from the Setup Component list Saving a Component Analysis Once all of the necessary components have been added click Save and enter a file name and location The component analysis will be saved as a cmp file Loading a Component Analysis To load an existing component analysis cmp file click Load and select the file This file may now be added to or edited If any changes are made to this file click Save to save the cmp file using the same file name and location or click Save As to use a different file name or location MENM NCCMNFIATEC Alf CECEnNDFCTM CANIM VI F A XA lt a A ES NI FI RK
11. e the ENV 590 Cylinder in the desired location for the assembled system as it is very difficult to move once it Is filled with water 2 Fill the ENV 590 Cylinder with water 3 Place the ENV 591 Test Cubicle around the ENV 590 Cylinder as shown in Figure 2 10 Figure 2 10 Cylinder Inside the Enclosure 4 Orient the Camera Plate as shown in Figure 2 11 Figure 2 11 Camera Plate Aligned MED ASSOCIATES INC FORCED SWIM 5 Secure the Camera Plate in place using the Alignment Plate Two of the pins on the Alignment Plate fit into the holes in the Camera Plate and the other two fit into the Cylinder base as shown in Figure 2 12 Figure 2 12 Assembled Hardware Adjusting the Camera Focus and Aperture Open the Video Monitor software application and open the Camera Window refer to the Video Monitor Users Manual To adjust the zoom loosen the thumbscrew on the ring and make the necessary adjustment Once proper image clarity is achieved tighten the thumbscrew on the ring It is recommended that the lens aperture be in the maximal open O position as shown below Figure 2 13 Camera Zoom and Aperture Rings ER MED ASSOCIATES INC FORCED SWIM Wiring Instructions 1 Using the FireWire cable connect the FireWire camera to the FireWire card Figure 2 14 FireWire Cable Connection Back of the Computer 2 Using the NIR 101 cable connect the To Light Panel connector on the back of
12. est Mouse H3 wmv Documenta E E Forced Swim 082508 Rat 013 E Forced Swim test Mouse 10 wmv E Forced Swim 082508 Rat Dit wmy E Forced Swim best Mouse 11 unn Forced Swim 082508 Rat 015 wmv El Forced Swim test Mouse I2 wmv E Forced Swim 082608 Rat 010 ww El Forced Swim test wms E Forced Swim 082608 Rat 011 worm Forced Swim 082608 Rat 012 ww Forced Swim 082608 Rat 013 wmv E Forced Swim 082608 Rat 014 wms Forced Swim 082608 Rat 015 wrnw E Forced Swim test Mouse HO wmv by Network Files of type video files wiv Cancel Places e Open as read only Next select the video wmv file to analyze and click Open The Analyzing screen shown in Figure 4 2 will appear When this screen disappears an fsa file has been created using the same file name and location as the wmv file Figure 4 2 Analyzing Screen with Status Bar Analyzing File 1 OF 1 File Name Forced Swim kest Mouse Hz wms Complete Opening a Motion Data fsa File Once a motion data fsa file has been created it can be opened using the Video Monitor Plug In for Forced Swim From the Main Screen Figure 3 1 select File Open The screen shown in Figure 4 3 will appear Select the file to open and click Open The Main Screen will appear as shown in Figure 4 4 Figure 4 3 Select the Motion Data fsa File to Open Select the force swim data file to open A Forced Swim 082508 Ra
13. menu item For the example selecting Zoom Rect XY allows the user to use the cursor to click and drag over the area to zoom in on as shown in Figure 4 5 This will result in the data being zoomed in on along both the X and Y axes as shown in Figure 4 5 Zoom Rect XY ay Forced Swim C Documents and Settings Forced Swim Desktop Forced Swim 082508 Forced Swim test Mouse 10 fsa x Experiment Subject Group Trial Total Time s 359 9 Thresholds Lower A upper B Time s Samples Sum y Above Upper Threshold 1 633 49 168501 a Between Thresholds 93 533 2806 5219513 gt Time s pee Below Lower Threshold 264 733 7942 5510981 4000 lr 3000 2000 4 1 1000 AA Experiment Subject Group Trial Total Time s 359 9 Thresholds Lower SA upper B Time s Samples Sum Above Upper Threshold 1 633 49 168501 Between Thresholds 93 533 2806 5219513 Below Lower Threshold 264 733 7942 5510981 Analyzing Data With the fsa file open and the Motion Index Graph properly zoomed in place the Upper and Lower Thresholds in the appropriate locations If the Range Cursors are being used they should be placed in the desired locations on the Motion Index Graph The data contained in the Experiment Data portion of the main screen are calculated using the Threshold and Cursor positions and are updated each time a Threshold or Cursor Is moved Any relevant text ma
14. olds and Time Cursor Chapter 3 Upper and Lower Thresholds and Time Cursor Chapter 3 Motion I ndex Graph Chapter 3 Motion Index Graph Chapter 3 Motion Index Graph Chapter 3 Motion Index Graph Zoom Menu Figure 3 4 Zoom Menu File Graph Component Analysis Help Previous Chri 2 Extents Ctrl E 4 Extent Y Extent Reck sY Ctrl F s Axis Onl Y Axis Only Menu Description Item P Previous Return to the previous zoom selection Extents Automatically zooms so that the motion data fills the graph area X Extent Automatically zooms so that all of the x axis time data is visible in the graph area Y Extent Automatically zooms so that all of the y axis motion data is visible in the graph area Allows the user to click and drag over the desired view area to zoom along both the Rect XY X and Y axes X Axis Allows the user to click and drag over the desired view area to zoom along the X axis Only only Y Axis Allows the user to click and drag over the desired view area to zoom along the Y axis Only only Component Analysis Menu Figure 3 5 Component Analysis Menu Component Analysis Help Fil Graph Zoom Setup Run Component Analysis Menu Item Description For More I nfo Chapter 5 Setup Opens the Component Setup screen l Component Analysis Chapter 5 Running a Run Component Analysis Opens the Component Summary Report screen Component Analysis Help Menu Figure 3 6 Helo Menu Fie Graph Zoom Component Anal
15. onitor software General Computer Environment e 3 4 GHz Processor e 1 GB RAM e Windows 2000 SP4 XP or Vista e FireWire Card e FireWire Camera s MED ASSOCIATES INC CHAPTER 2 Assembly Instructions Hardware Guide FORCED SWIM Figure 2 1 ENV 590 Cylinder MED ASSOCIATES INC Figure 2 3 Camera Plate Figure 2 4 Alignment Plate Figure 2 5 NIR 100 Light Controller Front Panel med JSSOCIJ ES INE White Light NIR Light Remote e ON Remote On NIR 100 FORCED SWIM MED ASSOCIATES INC FORCED SWIM Figure 2 7 NIR 100R Light Panel Light Panel Installation Refer to Figure 2 8 when completing these steps Figure 2 8 Installing the Light Panel 1 Orient the NIR 100R Light Panel so that the light is facing into the test cubicle and the connector is facing down 2 Place the NIR 100R Light Panel inside the groove on the back of the test cubicle 3 Use the four brackets and included screws to secure the light panel to the back of the test cubicle MED ASSOCIATES INC FORCED SWIM Installing the Camera Mount Refer to Figure 2 9 when completing these steps Figure 2 9 Installing the Camera Mount 1 Orient the ENV 598 Camera Mount over the pre drilled holes in the Camera Plate 2 Secure the ENV 598 Camera Mount in place using the included screws 3 Thread the camera onto the Camera Mount MED ASSOCIATES INC FORCED SWIM Hardware Assembly 1 Plac
16. pany Mame Company Name Password Password Issued When Software Registered The Choose Destination Location screen shown below appears next Verify the Destination Folder If this folder is acceptable click Next to continue If it is not click Browse and browse to the desired folder Figure A 4 Choose Destination Location Screen Forced Swim InstallShield Wizard Choose Destination Location Select folder where setup will install files Setup will install Forced Swim in the following folder To install to this folder click Nest To install to a different folder click Browse and select another folder Destination Folder C Program Files MED Associates Forced Swims MED ASSOCIATES INC FORCED SWIM The Ready to Install the Program screen shown below will appear next Click Install to begin installation Figure A 5 Ready to Install the Program Screen Forced Swim InstallShield Wizard Ready to Install the Program The wizard ts ready to begin installation Click Install to begin the installation IF you want to review or change any of pour installation settings click Back Click Cancel to exit the wizard InstallShield The software installation is now complete Click Finish to close this screen Figure A 6 Installation Complete Forced Swim Install Wizard med ASSOCIATES ME Install Wizard Complete The Install Wizard has successfully installed Forced Swim Click Finish to e
17. se H3 fsa m Forced Swim test Mouse 10 Fsa Forced Swim test Mouse I1 Fsa Forced Swim test Mouse 12 Fsa D My Network Files of type Forced Swim Analysis Files fsa Cancel Places Open as read only The component analysis is now complete The output csv file can be viewed using Microsoft Excel An example Component Analysis csv file is shown in Figure 5 7 Figure 5 6 Component Analysis Complete Forced Swim Example csv File The Component Analysis file contains the Counts and Times Below the Lower Between and Above the Upper Thresholds for each Component Any text that was entered in the Experiment Subject Group and Trial fields on the Main Screen will appear in this file as well as the Name Start Time Duration Start Frame and Duration Frames for each Component Figure 5 7 Example Component Analysis File Date 9 3 2008 13 31 Threshold 1 3444 Threshold 2 8453 Component Details Component Name Start Time Duration s Start Frame Duration Frames 1 00 00 0 60 0 1800 2 01 00 0 60 1800 1800 3 02 00 0 60 3600 1800 4 03 00 0 60 5400 1800 5 04 00 0 60 7200 1800 Total 00 00 0 300 0 9000 Samples Time Experiment subject Group Trial Component Name Below Low Threshold Between Thresholds Above Upper Threshold Below Threshold 1 Between Thresholds Above Threshold 2 Forced Swim Test Rat1 Group 1 Testing 1 1 71 1465 264 2 37 48 83 8 8 Forced Swim Test Rat1 Group 1 Testing 1 2 186 15
18. t 010 fsa ES Forced Swim 082608 Rat 014 analyzed fsa My Recent 3 Forced Swim 082608 Rat 014 fsa Dec Forced Swim test Mouse HO Fsa Forced Swim test Mouse H1 Fsa Forced Swim test Mouse H2 Fsa Desktop Forced Swim test Mouse H3 fsa Forced Swim test Mouse I0 fsa Y Forced Swim test Mouse I1 fs Forced Swim test Mouse 12 Fsa My Documents hy ansia a File name Y My Network Files of type Force Swim Data fsa Cancel Pl aces Open as read only Figure 4 4 Open fsa File Forced Swim C Wocuments and Settings Forced SwimWesktop Forced Swim 082508 Forced Swim test Mouse 10 fsa ES File Graph Zoom Component Analysis Help Experiment Subject Group Trial Total Time sh 359 9 Thresholds Lower BF Upper O B Time 5 Samples Sum Above Upper Threshold 1 633 49 168501 Between Thresholds 23 533 2506 5219513 w b l l gt H Time 5 0 03 Below Lower Threshold abi EES Pde 2910981 Zooming in on the Motion Index Graph When a motion data fsa file is initially opened the Motion Index Graph will be displayed so that all of the Motion Index data is contained within the Graph Display as shown in Figure 4 4 It may be necessary to adjust the zoom to better view the data There are several options on the Zoom menu that allow the user to optimize data viewing Please refer to Chapter 3 Zoom Menu for definitions of each
19. xit the wizard Forced Swim frumentation and software for research Finish Cancel 27
20. y be entered in the Experiment Subject Group and Trial fields Saving a Motion Data fsa File Saving a Motion Data fsa files will retain the current Threshold and Cursor positions as well as any text entered in the Experiment Subject Group and Trial fields To save the Motion Data file using the same file name and location click File Save To save the Motion Data file using a different file name and or location click File Save As CHAPTER 5 Component Analysis One may choose to compare data across different events within a single experimental session For example one may choose to report immobility in one minute increments over the course of a session This can be done using Component Analysis Adding Components To begin open an fsa file and select Component Analysis Setup The Component Setup screen shown in Figure 5 1 will appear To define each component click on the Add button and the Component screen shown in Figure 5 2 will appear Figure 5 1 Component Setup Screen Component Setup Component Name Start Time Mame Start Time 5 0 CO Add New Cancel Name each component and enter the Start Time s and the Duration s The Counts and Times Above Between and Below the Thresholds will be calculated for each component and the Start Time and Duration of the component determine the parameters for the calculation Click OK to add the new component and close the Component screen or click A
21. ysis About Menu Item Description For More I nfo Displays the software version and copyright About E information Screen shown in Figure 3 7 Figure 3 7 About Screen About Forced Swim Forced Swim version 1 0 Copyright fc 2008 MED Associates Inc med All rights reserved Motion Index Graph The subject s behavior is quantified as an ndex of motion All movement within the forced swim chamber is registered by the software as a change in video pixel composition over time Therefore robust and fast movements will be recorded as a large relative changes in video pixel composition and small refined and slow movements will be registered as somewhat smaller relative changes in pixel composition During analysis movement is represented as a graph motion index vs time in a non cumulative manner Upper and Lower Thresholds and Time Cursor The Motion Index Graph also contains Upper and Lower Thresholds and the Time Cursor The location of the Upper and Lower Thresholds is user defined and can be adjusted by either clicking and dragging the threshold lines to the desired location be sure that Move Cursors is selected on the Graph menu or by entering the motion index values into the Thresholds Lower and Upper fields on the main screen Figure 3 1 The Time Cursor is used to indicate the current place in the video and its location corresponds to the Time s field under the Video Display Window Figure 3 13 The video can

FORCED SWIM SYSTEM - Med Associates Inc.

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