CO2/pH Controller User`s Manual
Contents
1. repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service CAUTION This pH controller is not registered with the FDA and is not for clinical use on human patients J N caution Not for clinical use on human patients Publication 5405 001 REV C s Harvard Apparatus CO pH Controller User s Manual Specifications Specifications Range Resolution Accuracy Input Resistance Calibration Offset Slope CO Flowmeter Gas Input Output Connectors pH Analog Output Electrode Response Time Electrode Slope Electrode Impedance Electrode Stability Electrode Dimensions L x D System Power Dimensions H x W x D Weight Publication 5405 001 REV C 0 to 14 pH 0 01 pH 0 02 pH 10 Q 2 pH through offset trimmer 80 to 110 through slope trimmer 2 L per min Quick disconnect fittings BNC connector 4 20 mA 0 sec 55 mV pH in pH range of 1 to 14 200 MQ 0 05 pH day 1 5 x 0 03 in 38 1 x 0 75 mm 12 VDC Universal 90 240 VAC 50 60 Hz adapter 12 2 x 15 2 x 12 7 cm 4 8 x 6 x 5 in 0 8 kg 1 75 Ib Harvard Apparatus CO pH Controller User s Manual Unpacking Remove the pH controller and its components from the packing material Inspect the controller unit and components for damage If any damage is found contact Harvard Apparatus and do not use equipment unless otherwi2. 00 MQ Stability 0 05 pH day Dimensions L x D 1 5 x 0 03 in 38 1 x 0 75 mm Catalog No Product BS4 69 0494 Combination pH Electrode for Micro Wells Publication 5405 001 REV C
3. Basics Turning pH Controller On Make sure all connections have been properly made to the pH controller unit e 12 VDC connection e CO input hose e Analog Out cable to recorder or other pH Electrode e CO output hose Depress the I side of the switch on the rear of the pH controller unit When the pH controller is powered the Power On LED should illuminate pH Probe Calibration The CO 2 pH Controller has been calibrated with an OFFSET for 7 0pH and a SLOPE for 4 0pH with a set up point of 6 50pH Refer to the pH meter s user manual included for instructions on performing a 2 point or 3 point calibration Required Materials for Calibration Three pH buffer solutions with pH of 4 0 7 0 and 9 21 The micro pH probe provided is extremely sensitive and should be calibrated daily to ensure accurate readings Due to the high sensitivity of the probe electro static dis charges can create false spikes in the pH readings For best results use the probe in a shielded environment and make sure that operator uses an anti static discharge device when ever possible Another method to wrap the electrode probe with aluminum foil shield If needed the aluminum foil can be grounded Electrostatic Discharge Mats Anti Static Wrist Strap Kit pH Set Point Configuration This CO2 pH controller is a unilateral pH controller which means that it only controls pH in one direction in this case it can only prevent the pH from bec
4. CO2 pH Controller User s Manual CO Gas pH Controller MAI 70 2116 HARVARD APPARATUS Publication 5405 001 REV C WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes a
5. oming too basic With this controller a pH set point can be entered which will tell the pH controller to prevent medium s pH from rising above this set point by dosing and acid carbon dioxide To learn how to configure a pH set point refer to the pH meter s user manual included Flow Control Configuration The flow control is located on the front of the pH controller It determines the flow rate at which the CO is delivered to the medium Increasing the flow rate will reduce the response time of the pH controller and increase the pH overshoot of the con troller The overshoot of the pH controller is defined as the amount of pH that the controller will reduce the medium s pH below that of the configured set point There will always be some overshoot that occurs when CO is delivered because Publication 5405 001 REV C Harvard Apparatus CO pH Controller User s Manual Operating Basics once the CO flow to the medium is shut off some CO will still diffuse into the medi um thus further reducing the pH The desired flow rate for a particular medium will depend heavily on the buffering capabilities of the medium itself Mediums with weak basic buffering capabilities will respond quickly to the addition of the acid and will have a larger overshoot Mediums with strong basic buffering capabilities will respond slower and have a smaller pH overshoot There is also a flow control switch which controls the internal gas flo
6. rence and it is designed to fit small perfu sion chambers without effecting fluid exchange rate The electrode is pre filled with reference solution 3M NaCl solution in 25 glycerol There is no need to refill the reference electrode If the tip of the reference electrode is blocked open the luer cap and apply pressure to the luer port The reference solu tion will appear at the tip seen under a 10x magnification lens Fill the Tee fitting and put the luer cap back in its place Calibration 1 Connect the pH electrode to the BNC input of the pH meter 2 Place the electrode tip in a pH 7 00 buffer and set the meter OFFSET to pH 7 00 3 Rinse the electrode tip with distilled water 4 Place the electrode tip in a pH 4 00 buffer and set the meter SLOPE to pH 4 00 5 Place the electrode tip in the pH 7 00 buffer to confirm the settings One can choose the mV mode and plot the pH vs the corresponding mV values Luer Cap Reference Electrolyte Plastic Holder Reference Electrode Coax Cable BNC 4 ft a Shaft 1 5 in L pH Electrode Tee Luer Fitting 0 5 mm OD 0 75 mm OD This miniature combination pH reference electrode has been designed to fit small perfusion chambers without effecting fluid exchange rates It does not require an external reference electrode The reference solution can be replaced The electrode is stored wet Specifications Response Time 10 sec Slope 55 mV pH range of 1 to 14 Impedance 2
7. s they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 5 Harvard Apparatus CO2 pH Controller User s Manual Table of Contents SUBJECT PAGE NO Warranty and Repair Information cc 2 Specifications iviena innin enine daekan a dances 3 Unpacking ssssssssnnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn mnnn 4 Connections PIOMDIMNG sisicrrisiupiniciodertaritannniecniatedeeeiiibiciadiass 5 Electrical sinenstensttetesensdininanthabsceriubereretsiacetiooiages 5 Operating Basics Turning pH Controller On ccceeeeeeeeeeeeeeeeeees 6 pH Probe Calibration ccccccccsssssssseeeeseeeeeeees 6 pH Set Point Configuration cceeeeeeee 6 Flow Control Configuration 6 7 9 lt 1 21110 Naenae ee ee enn nO aN er T OT 7 Combination pH Electrode for Micro Wells 8 Publication 5405 001 REV C Harvard Apparatus CO2 pH Controller User s Manual Warranty and Repair Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This
8. se specified by Harvard Apparatus Your pH Controller System Includes e 1 Controller unit e 1 Micro pH probe 69 0494 Twin axial BNC connector for analog out 1 Output Hose I D medical grade tubing 1 AC DC power adapter 1 Power Cord Required for Use Length of I D tubing to connect CO Gas source and CO Input on rear of the pH controller unit Publication 5405 001 REV C s Harvard Apparatus CO2 pH Controller User s Manual Connections Plumbing CO In CO input This port requires a 4 ID flexible tubing Input pressure source should be regulated to 10psi Max e CO Gas Output This connection requires an output hose with a quick discon nect fitting supplied with the unit The output gas should be delivered directly to the cell culture this is best accomplished by using Harvard s PDMD Electrical 12 VDC The pH controller requires 12 VDC power Use only the adapter pro vided with the pH controller unit pH Electrode Input This is an isolated BNC connector that receives the input signal from the micro pH probe 69 0494 Analog Out This twin axial BNC connector carries the data out signal 0 to 20 mA or 4 to 20 mA for use with data acquisition system or chart recorder CO5 pH Controller Front View HARVARD COo pH Controller Back View Publication 5405 001 REV C 4 Harvard Apparatus CO2 pH Controller User s Manual Operating
9. w valve There are three possible settings for this switch Manual Auto and Momentary Manual Up Regardless of the pH set point the flow of CO gas is continu ous The flow rate will be controlled by adjusting the regulator LED will remain lit Auto Center The CO pH controller will use the set point to activate the on off flow of COz gas automatically LED will lit only when the valve is acti vated and delivering CO gt Momentary Down CO will flow as long as the switch is depressed When the switch is released it will return to the automatic mode the LED will lit as long as the switch is depressed Operation Configuration and Setup 1 AW WN Make all of the appropriate connections to the CO2 pH controller unit Power Hoses and Probe Calibrate the micro pH probe Configure the pH set point Set the flow control Place the flow control switch in the Auto position Ensure that CO can be delivered directly to the medium We recommend using Harvard Apparatus s PDMI to guarantee proper CO distribution over the medium Other than the initial configuration and setup the pH controller needs no additional input to perform The CO 2 pH controller will monitor the pH and automatically make adjustments depending on the pH set point Publication 5405 001 REV C 3 Harvard Apparatus CO2 pH Controller User s Manual Combination pH Electrode for Micro Wells The pH electrode requires no external refe
10. warranty does not extend to damage resulting from misuse neglect or abuse nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and
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