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AutoSelect AS50 Autosampler (USB) Operator`s

1. To Column To Column Sample Sample Wd de 5 xdi 2 To Waste From Pump To Waste From Pump Figure E 2 Injection Valve Flow Schematics Eluent flows through either the Load or Inject path depending on the valve position Inthe Load position sample flows from the AS50 inject port line through the valve and into the sample loop where it is held until injection Eluent flows from the pump through the valve and to the column bypassing the sample loop Excess sample flows out to waste e In the Inject position sample is swept to the column for analysis Eluent flows from the pump through the sample loop and on to the column carrying the contents of the sample loop with it E 2 Doc 031935 01 4 04 E Simultaneous Injections E 2 Connecting the Injection Valves 2 1 Connecting to Valves Installed in the IC System Modules This section explains how to plumb two IC systems in parallel 1 Locate the Y connector inside the AS50 autosampler compartment Y Connector Figure E 3 Simultaneous Injection Y Connector 2 Route the two lines from the Y connector through the side slot on the 550 3 Connect of these lines to port S 5 on the injection valve of the first ICS 1000 1500 2000 see Figure E 4 4 Connect the other line to port S 5 on the injection valve of the second ICS 1000 1500 2000 Doc 031935 01 4 04 E 3 A
2. To Column in System 1 To Waste From Pump in System 1 Figure E 6 Injection Valve Connections for Simultaneous Injection Valves Installed in the AS50 Doc 031935 01 4 04 E Simultaneous Injections E 3 Specifying the Plumbing Configuration 1 Press Menu 5 and 3 to go to the PLUMBING CONFIGURATION screen 2 Move the cursor to the SAMPLE SYRINGE VOLUME field and press Select A or Select to select the size of the sample syringe installed in the AS50 either 5000 or 10000 uL see Figure E 7 Press Enter When you select a sample syringe size of 5000 or 10000 uL simultaneous injection option functions are automatically enabled For example in the PLUMBING CONFIGURATION screen the LOOP SIZE parameter now displays fields for both injection loops see Figure E 7 3 Move the cursor to the LOOP SIZE V1 field and enter the size of loop installed on injection valve 1 Move the cursor to LOOP SIZE V2 and enter the size of loop installed on injection valve 2 Press Enter PLUMBING CONFIGURATION INJ ECT VALVE PEEK DOOR SIZE Vl 25 0 V2 25 08 SAMPLE SYRINGE VOLUME 5000 uL SAMPLE SYRINGE CONFIG READY PREP SYRINGE VOLUME 5 mL PREP SYRINGE CONFIG READY Help Prompt Figure E 7 Plumbing Configuration Screen Simultaneous Injection Mode NOTE If the ASS0 is controlled by Chromeleon the AS50 communicates the syringe size selected in the PLUMBING CONFIGURATION
3. 3 37 3 11 6 Deleting a Schedule 3 37 3 11 7 Copying a Schedule 3 37 3 12 Choosing the Injection Type 3 38 3 12 1 Full Loop Injections 3 40 Doc 031935 01 4 04 iii AutoSelect AS50 Autosampler 3 12 2 Partial Loop Injections 3 42 3 12 3 Partial Loop Limited Sample Injections 3 44 3 13 Adjusting the Sample Syringe Speed for Different Sample NISCOSIUCS eed dhs wie erady sm UR MNA hee 3 45 3 14 Routine Maintenance 3 46 3 1451 Daly pee ee ee SI 3 46 3 14 2 Periodically He a teaches e dose dene ee Pt td 3 46 3 14 3 Annually 3 47 3 14 4 Manually Flushing the Inject Port 3 48 3 15 System Shutdown 2 iee cee tee eee nn 3 48 4 Troubleshooting 4 1 Error Messages celu eia Sabon pA RING Sees m Kaye mE m Xu hg 4 1 4 2 Liquid Leaks ein rine eim RE AES RA RUN 4 21 4 2 1 Leaking Syringe or Syringe Valve Port 4 21 4 2 3 Leaking Drain Line Connection 4 22 4 23 Leaking Inject Port 0 eee eee 4 22 4 24 Leaking Fitting cee eee eee 4 22 4 25 Liquid Line 4 22 5 Service 5 1 Replacing Tubing and Fi
4. 5 29 Replacing the Prep Syringe Valve 5 31 Changing the Main Power Fuses 5 33 Using the Chromeleon Wellness Panel 5 34 5 15 1 Opening the Wellness 1 5 34 Doc 031935 01 4 04 AutoSelect AS50 Autosampler A Specifications A l A 2 Autosampler sco so cesar es ea UR EE se a VERBO ER EN A 1 All Blecthicals c020 saver an be ME m as 1 1 2 1 1 1 3 Valves 2 AWA Mjetin osos ena bebe A 2 Sample Temperature Control Unit Optional A 3 B Installation B 1 B 2 B 3 Facility B 1 2 Autosampler 3 B 3 1 Before You Begin B 3 B 3 2 Installation B 3 B 3 3 Connecting the Syringe s and Drain Line B 4 B 3 4 Connecting the Injection Valve B 8 B 3 5 Connecting the 550 to the Chromeleon PC Optional B 10 B 3 6 Connecting the Power B 12 3 7 Turning on the Power 0 0 0 cee eee eee eee B 13 B 3 9 Module Setup o zT pe ke ee be ire
5. Sample Syringe Front Fitting Valve Prep Syringe Sample Transfer Line Reagent B Optional Sample Syringe Syringe Reagent C VM Optional Sampling Reagent D Reagent A Flush Reservoir Needle Optional Figure 2 20 Fluid Schematic for Sample Syringe with Optional Prep Syringe 2 32 Doc 031935 01 4 04 2 Description 2 6 8 Understanding the Status Display During a Run During runs the MAIN STATUS and DETAIL STATUS screens display the operation s currently being performed Because of sample prep overlap information on multiple injections may be displayed simultaneously at certain points in the schedule For example in Figure 2 21 the AS50 is injecting the sample and performing the timed events for the first injection from vial 1 while preparing for the second injection Method Timed Events Preparing for Elapsed Time Clock for Injection 1 Injection 2 Since Injection 1 DETAIL FLUSHING VIAL 1 12 5 ram AP WIE INJ 1 3 VODS25 uL METHOD 12 53 TRAY 2 mis INIEY a 25 20 LOOP 100 uL RLY2 COl PARTIAL INJ VLV LOCAL SiG 1 GSD Help Prompt Figure 2 21 Detail Status Screen Example Notes The INJ field is updated when the AS50 loads the loop for the next injection The TIME field and the METHOD timed events clock are reset to zero at injection The TIME field counts up from the injection until the next injection occurs
6. Area Area Area Area Area Peak Table Doc 031935 01 4 04 E 15 AutoSelect AS50 Autosampler E 16 Doc 031935 01 4 04 F Unpacking Instructions Figure 1 Fully packed AS50 with foam spacer on top of geometric foam pack 1 Remove the outer cardboard sheath and Ship Kit spacer see Figure 2 Figure 2 AS50 and Ship Kit with the outer cardboard sheath and Ship Kit spacer removed Figure 3 AS50 with foam top cap installed 2 Remove the foam top cap Doc 031935 01 4 04 F 1 AutoSelect AS50 Autosampler Figure 4 AS50 with sleeve assembled over cradle Note The ASSO is inserted into the bottom foam of the main shipping container The tubing is coiled in front of the AS50 3 Using the hand holes in the cradle place the 550 on the bench 4 Remove the sleeve see Figure 5 Figure 5 AS50 setting on cradle with the sleeve removed Note The rear edge of the cradle and the rear edge of the AS50 are aligned Figure 6 AS50 fully wrapped and taped within polyethylene bag 5 Remove the polyethylene bag see Figure 7 Be careful not to damage the drain tube F 2 Doc 031935 01 4 04 Unpacking Instructions Figure 7 AS50 sitting on foam spacer inside polyethylene bag Note The rear of the 550 is aligned with the rear edge of the foam Figure 6 2 in w
7. Doc 031935 01 4 04 F 5 AutoSelect AS50 Autosampler Figure 16 Polyethylene sleeve assembled clipped to shaft to eliminate sliding of sample arm during shipment 13 Remove the clip and sleeve see Figures 17 and 18 Figure 17 Polyethylene sleeve and clip Figure 16 Shaft with polyethylene sleeve removed F 6 Doc 031935 01 4 04 A A failure has occurred during power up 4 3 A NAK command was received 4 7 Aborting a schedule 2 4 3 11 Adding timed events steps 3 29 Adjusting the sample syringe speed 3 45 Aligning the inject port 5 19 Aligning the sampling needle 5 19 Ambient operating temperature A 1 An attempt was made to move a syringe valve 4 5 An attempt was made to move the syringe 4 5 An attempt was made to set an illegal syringe speed 4 6 An attempt was made to use the sample prep syringe 4 3 An illegal vial number was specified 4 7 Anion E 14 Applications 3 1 Arrow buttons 2 5 Automated control 2 19 See also Chromeleon Automatically flushing the inject port 2 30 Autosampler compartment 2 8 B Backlight C 2 C 20 Battery 5 1 Battery backed up RAM failed 4 9 Baud rate C 45 Beeping when key is pressed C 20 BIOS code revision level C 31 Blank screen C 2 C 20 Bubbles Removing from the syringe 5 4 B 21 Index Buttons Cursor arrows 2 5 Delete 2 5 Door 2 4 Enter 2 5 Help 2 5 2 7 Hold Run 2 4 Home 2 4 I
8. Temperature Statistics screen C 35 Tests Diagnostic C 32 Theory of operation 2 22 Time Setting the time and date B 15 C 23 Time between injections 3 27 Specifying 2 26 3 27 Time Function In screen C 30 C 46 Time since last injection C 5 Time zero step 3 29 C 15 Time Date screen C 23 Timebase For simultaneous injections E 10 Timed events 3 29 C 15 Defining 3 28 Deleting steps 3 29 INIT step 3 29 C 15 Time zero step 3 29 C 15 Timed events clock Display during a run 2 33 During schedule hold 3 10 Starting 3 29 Timed events step took too long 4 11 Timed out waiting for home position to be found 4 12 Timed out waiting for motion complete command 4 8 Timed out waiting for tray temperature to stabilize 4 20 Timeout occurred waiting for syringe 4 4 4 5 Tray Covers for 3 2 Loading 3 1 Types of 2 11 Tray temperature Setting 3 15 3 27 See also Sample temperature control option Tray types 2 11 C 7 Valid vial numbers in methods 3 19 Troubleshooting 4 1 Error messages 4 1 Liquid leaks 4 21 Doc 031935 01 4 04 Index 11 AutoSelect AS50 Autosampler TTL inputs Control of 2 16 C 30 D 2 Function assignments C 30 D 2 TTL signal mode C 30 TTL relay connector 2 15 D 1 Connection instructions D 2 Pin assignments D 1 TTL relay control Direct control of outputs C 7 Method control of outputs 3 31 Module setup for B 16 TTL relay outputs Controlling 2 17 D 5 Specific
9. The METHOD timed events clock counts up until all timed events have been performed for an injection The clock then stops and is no longer displayed Doc 031935 01 4 04 2 33 AutoSelect AS50 Autosampler 2 34 Doc 031935 01 4 04 3 Operation and Maintenance 3 1 Getting Ready to Run The AutoSelect AS50 Autosampler is designed for use with IC ion chromatography and BioLC biocompatible liquid chromatography applications and should not be used for any other purpose Operation of the AS50 in a manner not specified by Dionex may result in personal injury If you have a question regarding appropriate usage contact Dionex before proceeding In the U S call 1 800 346 6390 and select the Technical Support option Outside the U S call the nearest Dionex office 3 1 1 Fill the Vials and Load the Sample Tray 1 Fill the vials to the vial Never fill shoulder or lower see 9 above R this point Figure 3 1 Do not fill to the E top During sampling make sure the needle s vent is not immersed in liquid For 10 mL vials only If necessary raise the needle height inside the vial to position the vent above the liquid see Controlling the Needle s Position in the Vial 2 Needle vent on page 3 27 Do not immerse All vial sizes 10 mL vial Figure 3 1 Maximum Level to Fill Vials Doc 031935 01 4 04 3 1 AutoSelect AS50 Autosampler Screw a cap
10. The temperature is stabilized T temperature is increasing V temperature is decreasing Displays the measured Peltier currents Doc 031935 01 4 04 C 35 AutoSelect AS50 Autosampler Screen Field Description CONTROL Displays the state of the Peltier control bits bit 0 Saturate O duty cycle 1 saturate bit 1 Peltier enable 1 on bit 2 Heat cool O heat 1 cool bits 3 7 Always 0 DUTY CYCLE Displays the Peltier duty cycle from 0 through 255 C 36 Doc 031935 01 4 04 C Display Screens C 9 5 Liquid Control Screen Use the LIQUID CONTROL screen to e Manually control the sample and prep syringes e Manually control the inject valve Manually position the needle at a specified vial The AS50 must be in Local mode Direct Control to manually control these functions ASPIRATE Help Prompt Figure C 25 Liquid Control Screen ASPIRATE Help Prompt Figure C 26 Liquid Control Screen Simultaneous Injection Mode VIAL The position for the sampling needle Press Select A or Select V to cycle through vial numbers and options Use numeric buttons to enter an absolute vial number The range of valid vial numbers depends on the type of tray currently installed Press Enter to move the needle to the selected position Absolute vial numbers default 1 INJ inject port FLU flush port Doc 031935 01 4 04 C 37 AutoSelect AS50 Autosampler Scree
11. Figure 5 21 Removing the Syringe Valve 5 28 Doc 031935 01 4 04 5 Service 5 12 Replacing the Sample Syringe Valve 1 Disconnect the liquid lines from the existing valve 2 Remove the existing valve see Section 5 11 3 When the valve is removed the syringe drive shaft is visible When the syringe is in the initial position the position selected when the valve was removed the slot in the drive shaft should be horizontal see Figure 5 22 Sample Syringe Drive Shaft Initial Position Figure 5 22 Sample Syringe Drive Shaft Initial Position If the slot in the drive shaft is not horizontal the syringe drive may need replacing Contact Dionex for assistance Do not attempt to manually turn the drive shaft 4 Check the back of the new sample syringe valve P N 054242 The valve drive shaft should be perpendicular to valve ports A and D see Figure 5 23 If it is not rotate the valve rotor until the drive shaft is in the correct position Port A On Front Drive Shaft Initial Position NS N i GY Back View c of Valve SS Port D On Front Figure 5 23 Sample Syringe Valve Drive Shaft Initial Position Doc 031935 01 4 04 5 29 AutoSelect AS50 Autosampler 5 10 11 Each valve port must contain a white Teflon washer Check the inside of each port and insert a washer P N 061315 if one is not already prese
12. Go to the SCHEDULE screen Position the cursor in the EDIT field and enter the number of the schedule to be copied Position the cursor in the SAVE TO field and enter a new unused schedule number Press Enter The parameters from the original schedule are copied to the new schedule and the original schedule is unchanged Doc 031935 01 4 04 3 37 AutoSelect AS50 Autosampler 3 12 Choosing the Injection Type Three types of sample injections are possible Full loop the full loop volume is injected e Partial loop a partial volume of the loop is injected e Partial loop limited sample a partial volume of the loop is injected and no extra sample is aspirated from the vial NOTE If the AS50 is set up for simultaneous injections only full loop injections can be performed see Section E 4 The table below summarizes the injection types See the following sections for details Injection Type Injection Cut Sample Volume Volume Volume Volume Used Injected Full loop 2 Loop volume Ignored 4 X Loop volume Loop volume or 2 5 X Loop volume 25 uL whichever is greater Partial loop lt Loop volume 1 30 uL Injection volume Injection 2 X cut volume volume from schedule Partial loop lt Loop volume 0 Injection volume Injection limited sample volume from schedule Injection volume is entered on the SCHEDULE screen see Section 3 11 4 or in the Chromeleon sequence in the Browser For the best a
13. receptacle on the AS50 connector into the USB rear panel port on the Chromeleon PC Figure B 8 Connecting the AS50 Directly to the PC If there are no unused USB ports on the PC connect 550 to an external USB hub P N 060392 and connect the Chromeleon PC to the hub see Figure B 9 NOTE USB connections require standard USB A to B cables Doc 031935 01 4 04 B Installation NOTE If the number of USB devices in the system exceeds the number of available USB ports connect the devices to the system with a USB hub P N 060392 One or more hubs is also required if the AS50 or other USB device is more than 5 meters 16 ft from the PC Refer to the manual provided by the hub vendor for complete installation instructions The USB standard limits the USB cable length to 5 meters 16 ft Each USB device can be separated from the PC by no more than five hubs Thus each USB device can be located no more than 30 meters 32 yds from the PC Other Other USB USB Device Device B USB Cables A Computer Running Chromeleon Plug a USB cable s B connector into the USB receptacle on the AS50 Plug the A connector into an A port on the USB hub Plug a USB cable s A connector into a USB port on the Chromeleon PC Plug the B connector into the B receptacle on the USB hub Figure B 9 Connecting the AS50 to a USB Hub
14. 3 Operation and Maintenance 3 7 Stopping a Running Schedule 1 To halt a schedule press Stop 2 When the following message appears press Stop again Press STOP again to abort Press HOLD RUN to resume Help Prompt Figure 3 8 Stop Message Screen When a schedule is aborted the AS50 performs the following sequence of operations The sampling needle arm moves to the waste port and empties any fluid from the needle The needle arm moves to the flush port and delivers a volume of flush fluid that washes the outside of the needle The needle arm then moves to the inject port where a volume of flush fluid is delivered for flushing the inject port The needle arm moves back to the flush port and the outside of the needle is again flushed The needle arm moves to the home position Doc 031935 01 4 04 3 11 AutoSelect AS50 Autosampler 3 8 Opening the Autosampler Door During a Schedule Normally the autosampler door must remain closed while the AS50 is running a schedule If the door is opened inadvertently the sampling arm stops immediately and the schedule is aborted If you want to open the door during a schedule first press the Door button on the front panel and then follow the on screen instructions If it is not safe to open the door at this time the following message appears STATUS EEUSHEING 250 UJ OPEN DOOR SAFETY SCREEN WARNING Presently it is not safe to open th
15. 8 400 min 10 500 min 10 700 min 12 200 min 15 400 min 18 000 min 0 250 AG External Lin Auto 0 250 AG External 0 250 AG External 5 000 RG External 0 250 AG External 0 250 AG External Lin QOff QOff QOff QOff Auto Auto Auto Auto Auto gt 4 Detection APeak Table A Amount Table Ped gt E 14 Doc 031935 01 4 04 E Simultaneous Injections If two anion or two cation detector channels were being used the measured retention time for each anion or cation would appear in both Ret Time columns as in the following example 5 90 9 _ AS4ASC Simultaneous Simultaneous _ASSHC_AS4ASC Es Peak Hame Ret Time Ret Time Ret Time Standard Int Type Cal Type Peak Type ECD 1 ECD 2 Ei 1 000 3 700 0 100 AG External Area Off Chloride 1 600 min 5 500 min 0 100 AG External Area GOff Auto itri 1 900 min 8 090 min 0 150 AG External QOff Auto 3 000 10 700 min 0 200 AG External QOff Auto 3 600 12 800 min 0 250 External QOff Auto 6 Chiorite 5 087 min 0 250 External QOff Auto 7 Phosphate 5 400 16 400 min 0 250 External QOff Auto 5 770 min 0 250 AG External QOff Auto 7 200 17 800 min 0 250 AG External QOff Auto 10 Chlorate 11 400 min 0 250 AG External Area GOff Auto ne 4 gt Detection Amount Table of Area Area
16. Button y N C Delete P J Select A ON ON Select A A Help CN a Menu Enter Function Delete performs several functions e Cancels an entry that is in progress and restores the previous value Returns a field to its default value if an entry is not in progress Deletes a line in a method or schedule To do this position the cursor in the leftmost field of the line that you want to delete and press Delete twice Deletes an entire method or schedule To do this position the cursor in the method or schedule edit field or in the time field of the INIT step and press Delete twice The Select buttons cycle through predetermined options in entry fields To confirm the selected value press Enter or move out of the field by pressing a cursor arrow button In fields that have predetermined numeric choices Select A increases the value by one unit and Select V decreases the value by one Holding down a Select button increases or decreases the value continuously The arrow buttons move the cursor in the direction of the arrow to the next entry field if one exists At the end of a line the left arrow wraps the cursor around to the next entry field on the line above the right arrow wraps the cursor to the next entry field on the line below The up and down arrows do not wrap around After entering a new value in an entry field pressing an arrow button save
17. Doc 031935 01 4 04 B 11 AutoSelect AS50 Autosampler B 3 6 Connecting the Power Cord Connect the power cord IEC 320 C13 ordered separately from the main power receptacle on the rear panel to a grounded power source The AS50 power supply is auto sensing so no adjustment is required to select the line voltage SHOCK HAZARD To avoid elecirical shock use a grounded receptacle Do not operate the AS50 or connect it to AC power mains without an earthed ground connection The power supply cord is used as the main disconnect device Make sure the socket outlet is located near the AS50 and is easily accessible gt gt 2 o CAUTION Operation at AC input levels outside of the specified operating voltage range may damage the AS50 gt CAUTION DANGER D ELECTROCUTION Pour viter toute lectrocution il faut utiliser une prise de courant avec prise de terre Ne l utilisez pas et ne le branchez pas au secteur C A sans utiliser de branchement mis la AVERTISSEMENT terre gt Le cordon d alimentation principal est utilis comme dispositif principal de d branchement Veillez ce que la prise de base soit situ e install e pr s du module et facilement accessible gt MISE EN GARDE STROMSCHLAGGEFAHR Zur Vermeidung von elektrischen Schlagen ist eine geerdete Steckdose zu verwenden Das Gerat darf nicht ohne Erdung betrieben bzw an Wechselstrom angeschlossen WARNUNG werden gt D
18. details 3 1 2 Turn On the Power Press the power switch in the lower left corner of the front door to turn on the AS50 power When the power is turned on a series of screens are displayed and the autosampler performs a power up sequence The COPYRIGHT NOTICE screen is displayed briefly and then the POWER UP screen appears While the POWER UP screen is displayed the AS50 performs initialization procedures and a series of diagnostic tests If one of the tests fails testing stops and the DIAGNOSTIC TEST screen is displayed See Section C 9 2 if this occurs When the initialization procedures are complete and the 550 has passed the diagnostics the INSTALLED OPTIONS screen appears After a few seconds or if you press a button the MAIN screen is displayed The power up sequence is then complete After power up you can display the POWER UP DIAGNOSTIC TEST and INSTALLED OPTIONS screens by selecting on the MENU of SCREENS See Appendix C Doc 031935 01 4 04 3 3 AutoSelect AS50 Autosampler 3 2 Overview of AS50 Screens Figure 3 3 shows the screens that are available for operating the autosampler Refer to Appendix C for details about each screen Figure 3 3 AS50 Menu Structure MENU of SCREENS 1 2 3 4 5 6 7 8 9 MAIN DETAIL METHOD SCHEDULE MODULE FLUSH TIME DIAGNOSTIC PRINT STATUS STATUS MENU SETUP MENU PRIME MENU FUNCTION OUT 1 SAMPLE 1 INSTALLED POWER UP PREP OPTIONS 1 PRINT SCH
19. 24 IONEX AutoSelect AS50 Autosampler USB Operator s Manual Now sold under the Thermo Scientific brand Thermo SCIENTIFIC Part of Thermo Fisher Scientific Document No 031935 Revision 01 April 2004 2004 by Dionex Corporation All rights reserved worldwide Printed in the United States of America This publication is protected by federal copyright law No part of this publication may be copied or distributed transmitted transcribed stored in a retrieval system or transmitted into any human or computer language in any form or by any means electronic mechanical magnetic manual or otherwise or disclosed to third parties without the express written permission of Dionex Corporation 1228 Titan Way Sunnyvale California 94088 3603 U S A DISCLAIMER OF WARRANTY AND LIMITED WARRANTY THIS PUBLICATION IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND DIONEX CORPORATION DOES NOT WARRANT GUARANTEE OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATIONS REGARDING THE USE OR THE RESULTS OF THE USE OF THIS PUBLICATION IN TERMS OF CORRECTNESS ACCURACY RELIABILITY CURRENTNESS OR OTHERWISE FURTHER DIONEX CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR CHANGES TRADEMARKS BioLC and Chromeleon are registered trademarks and PolyVial is a trademar
20. 3 10 3 Defining Sample Prep Steps Overview Use the SAMPLE PREP screen see Figure 3 13 to specify a sequence of operations to be performed before the method s timed events Six standard sample prep operations are available DELAY NEEDLE PIPET MIX FLUSH and WAIT In addition if the sample preparation option is installed DISPENSE and DILUTE operations are added Prep Operation DELAY NEEDLE PIPET MIX FLUSH DISPENSE DILUTE WAIT Description Specifies for how long the AS50 waits before proceeding to the next step in the sample prep phase The height of the needle above the bottom of the vial Moves a specified volume of sample from one vial to another Mixes the vial contents by repeatedly drawing and expelling a volume of sample Flushes the needle with a specified volume This operation is independent from the flush that takes place before each injection in a schedule see Section 2 6 6 Moves a specified volume of reagent from a reservoir to a vial Dilutes a volume of concentrate with a specified volume of diluent The 550 waits for a continue command before proceeding with the method The command can be from the front panel pressing Hold Run or a TTL input Except for WAIT each operation can be performed multiple times or not at all The sample prep steps must include a single wait step The wait operation can be disabled however from the SYSTEM PARAMETERS screen see Section C 6
21. Doc 031935 01 4 04 2 Description 2 9 1 Syringe and Reservoir Organizer The organizer on the top of the autosampler holds the sample syringe 1 L flush reservoir and sample preparation option prep syringe and reagent reservoirs see Figure 2 5 Sample Syringe Functions The sample syringe performs these operations e Pipettes liquid from one vial to another or to waste Mixes a vial by repeatedly drawing and expelling the vial contents If the AS50 is equipped with the sample preparation option mixing is done with the sample prep syringe instead of the sample syringe e Delivers sample to the injection valve Several sample syringe volumes are available Syringe Volume Part Number 100 uL 055064 250 uL standard 053916 500 uL 055065 1000 uL 055066 5 mL forthe simultaneous 053915 injection option only 10 mL for the 055068 simultaneous injection option only Sample Preparation Option Functions The AS50 can be equipped with a sample preparation option that includes a sample prep syringe a sample prep valve and one 250 mL reagent reservoir Additional reagent reservoirs are available The sample prep syringe performs these operations Dispenses reagents from external reservoirs to any vial Mixes a vial by repeatedly drawing and expelling the vial contents Doc 031935 01 4 04 2 9 AutoSelect AS50 Autosampler 2 3 2 IMPORTANT Performs dilutions allowing preparation of multilevel
22. Figure C 19 Time Function In Screen Description Select the signal mode that corresponds to the signal type of the controlling device NORMAL EDGE the default mode is compatible with the TTL output signals provided by Dionex modules Not active A signal to TTL2 from the controlling device starts the schedule if it is not currently running or continues the current schedule when the AS50 is at the wait step of a method A signal to TTL3 from the controlling device turns the optional sample temperature control unit on and off This TTL is for support of the thermal compartment option which is not currently available MODE EDGE Values NORMAL EDGE INVERTED EDGE NORMAL PULSE INVERTED PULSE Doc 031935 01 4 04 C Display Screens C 9 Diagnostic Menu To go to the DIAGNOSTIC MENU press Menu and 8 Help Prompt Figure C 20 Diagnostic Menu Screen C 9 1 Power Up Screen Use the POWER UP screen to display the revision levels of the Moduleware and USB BIOS code the module s ID number if connected and the serial number of the AS50 This is the same power up screen that displays when you turn on the AS50 Help Prompt Figure C 21 Power Up Screen Doc 031935 01 4 04 C 31 AutoSelect AS50 Autosampler C 9 2 Diagnostic Tests Screen This screen lets you test 550 electronics components PERDU P LEAKS Help Prompt DIAGNOSTIC TESTS P DSP COMM P SYRINGE COMM P
23. PRIME The volume of fluid used for priming 100 to 5000 uL VOLUME default 400 C 28 Doc 031935 01 4 04 C Display Screens Screen Field PRIME RESERVOIR PRIME SYRINGE PRIME ACTION Description Values The reservoir used for the priming cycle If the FLUSH RES A sample preparation option is installed press Select RES B RES C or Select V to cycle through the choices If the RESD sample preparation option is not installed the flush reservoir is always used and this field cannot be edited The syringe used for the prime cycle If the sample SAMPLE PREP preparation option is not installed the sample syringe is always used and this field cannot be edited To start a prime cycle press Select A or Select V to READY PRIME select PRIME and press Enter The AS50 must be in Direct Control mode before starting the prime cycle Doc 031935 01 4 04 C 29 AutoSelect AS50 Autosampler C 8 Time Function In Screen Use the TIME FUNCTION IN screen to Display the AS50 functions that can be controlled via TTL input from another device Select a TTL signal mode for each function See Appendix D for details about TTL controlled functions and connections START CONTINUE 2 TRAY TEMP ON OFF 3 COLUMN TEMP ON OFF 4 Help Prompt Screen Field MODE UNUSED TTL1 START CONTINUE TTL2 TRAY TEMP ON OFF TTL3 COLUMN TEMP ON OFF TTL4 TIME FUNCTION IN yr UNUSED 1 NORMAL
24. Y Y address C 26 Z Z address C 26 Doc 031935 01 4 04 Index 13 AutoSelect AS50 Autosampler Index 14 Doc 031935 01 4 04
25. 0 0 0 Z SENSOR 3 0 0 0 ZZ Ole 4 0 0 0 Help Prompt Figure C 23 XYZ Test Screen Screen Field Description ACTION The action the needle arm should perform HOME Homes all three axes GO TOX Positions the arm at the specified location 1 2 3 or 4 CYCLE Continuously cycles the arm through the four positions PATTERN Continuously cycles through a four corners test pattern with the needle moving about halfway into the vial VIALS Continuously cycles the arm through each position in the currently installed tray with the needle tapping the top of each vial ACTUAL XYZ Displays the needle arm s current XYZ position The numbers indicate the number of motor steps away from the home position XYZ1 2 3 4 Allows entry of XYZ values for four needle arm positions The numbers indicate the number of motor steps away from the home position X SENSOR Indicates the current state of the X and Y sensors The X and Y Y SENSOR sensors detect evenly spaced slots that indicate the home position and mark distance intervals Z SENSOR Indicates whether a vial is present When the needle is down and a vial is present 1 is displayed If no vial is present at the current location when the needle is down 0 is displayed Doc 031935 01 4 04 C 33 AutoSelect AS50 Autosampler Screen Field Description Z HOME Indicates whether the Z axis is at the home position An entry of 0 indicates home To run a test 1 M
26. From the top carefully insert the needle into the needle block see Figure 5 8 making sure the needle is vertical and centered over the opening inside the block Needle Cradle Figure 5 8 Installing the Needle in the Cradle Doc 031935 01 4 04 5 11 AutoSelect AS50 Autosampler IMPORTANT Continue inserting the needle making sure it is lined up with the opening in the cradle The needle stops when the fitting on the needle reaches the bottom of the needle block Insert the bolt into the needle block With one hand hold the tubing in place above the bolt to make sure the needle remains vertical and centered in the needle block and cradle With your other hand tighten the bolt fingertight To check the position of the needle inside the cradle hold the bolt above the needle block and lift the needle up all the way until it stops Then let it back down making sure it enters through the center of the needle block and is centered in the cradle Position the yellow sleeve on the tubing in the position measured in Section 5 3 1 Step 5 Orient the tubing clip up or down depending on the natural curve of the tubing and attach the clip to the tubing where the yellow sleeve is attached Screw the clip back onto the needle arm again Do not shorten the sample transfer line tubing The extra length is required to prevent sample from being pulled into the syringe during operatio
27. Help Prompt Figure B 10 Plumbing Configuration Screen Verify that PEEK is selected in the INJECT VALVE field Move the cursor to the LOOP SIZE field and use the numeric buttons to enter the size of the sample loop installed on the injection valve or on the first injection valve if the simultaneous injection option is installed Move the cursor to the SAMPLE SYRINGE VOLUME field and press Select A or Select V to specify the volume of the sample syringe installed When you select a sample syringe size of 5000 or 10000 uL IMPORTANT simultaneous injection option functions are automatically enabled For example in the PLUMBING CONFIGURATION screen the LOOP SIZE parameter now displays fields for both injection loops 6 If the sample preparation option is installed move the cursor to the PREP SYRINGE VOLUME field and press Select A or Select V to specify the volume of the prep syringe If the simultaneous injection option is installed move the cursor to the LOOP SIZE V2 field and enter the size of the sample loop installed on the second injection valve Press Enter Doc 031935 01 4 04 B Installation Set the Time and Date 1 From the MODULE SETUP MENU press 4 to go to the TIME DATE screen n 56 16 JUNE Help Prompt Figure B 11 Time Date Screen 2 The SET TIME field uses a 24 hour time format hour minutes seconds Press numeric buttons to enter the time directly or press Select A or
28. Method Setup 4 Execute INIT Step Load Loop 4 Events Method Time 0 0 Inject Start Cycle Timing Method Times x x x x Set TTLs Relays Switching Valve etc See Note 4 Method Finished See Note End of Cycle Time 33 Continue Yes See Note 2 Method Starts for Injection 3 4 Continue as Shown for Method 2 Until Last Scheduled Injection Completed 4 Flush Inject Port and Wash Needle 4 Schedule Ends No Sample Prep Do Sample Prep Pipetting Mixing etc 4 Wait for Previous Method to Finish 4 Wait for Continue Command 4 Method Setup 4 Execute INIT Step Load Loop i Wait for Cycle Time Method Time 0 0 Inject 4 Method Times x x x x Set TTLs Relays Switching Valve etc l Method Finished Notes 1 The schedule either stops and waits or skips to the next vial depending on the action selected in the schedule 2 If multiple injections are specified from the same vial the sample prep steps are perfomed once only before the first injection 3 The continue command can be from pressing the Hold Run button or from a TTL input 4 Use of Cycle Time is optional Figure 2 17 Order of Ev
29. Operating from 2 16 3 7 Index 4 Doc 031935 01 4 04 Index Front Panel screen C 20 Full loop injection 3 38 Setting up 3 41 Summary of 3 38 Fuses A 1 Changing 5 33 H Halting operation 3 11 Heating the sample tray 2 2 2 12 Help button 2 5 2 7 Hold Run button 2 4 Pausing a schedule 3 10 Starting a schedule 3 9 Home position 2 4 C 33 Home position syringe 5 7 Hub USB B 10 Humidity A 1 I IC systems Connections for simultaneous injections E 4 E 5 ICS 1000 1500 2000 Connections for simultaneous injections E 3 E 5 INIT step 3 29 C 15 Initialization procedures 3 3 B 13 Initializing the syringe 5 5 Inject port 2 11 Aligning 5 19 Calibrating the volume 5 23 Flushing 3 48 Leaking 4 22 Volume of C 25 Inject Port Alignment screen C 26 Injection types 3 38 Displayed C 7 Full loop 3 38 Partial loop 3 38 Partial loop limited sample 3 44 Injection valve Connections B 8 Controlling directly C 7 Controlling in a method 3 30 Controlling with Chromeleon 2 21 Flow schematic E 2 For simultaneous injections 2 13 E 2 Specifications A 2 Specifying PEEK or SST C 21 Injection volume C 18 Choosing the injection type 3 38 Setting with Chromeleon 2 20 Simultaneous injection mode C 18 Specifying 3 33 C 18 Specifying in a schedule 3 36 Injections Controlling the valve 3 30 Running a schedule 3 8 Specifications A 2 Types of 3 38 Valve flow description E
30. SYRINGE SPEED 3 ACTION ASPIRATE 8 Press Enter 9 Select VIAL FLU and press Enter The needle arm moves to the flush port 10 Select ACTION EMPTY and press Enter Doc 031935 01 4 04 5 23 AutoSelect AS50 Autosampler 11 12 13 14 15 16 17 18 19 20 2 Select the following options from FLUSH SYRINGE SPEED 5 ACTION FILL Press Enter Select the following options DISPENSE 50 uL SYRINGE SPEED 5 ACTION DISPENSE Press Enter Select VIAL INJ and press Enter The needle arm moves to the inject port Select the following options DISPENSE 1 uL SYRINGE SPEED 1 ACTION DISPENSE Press Enter The syringe dispenses 1 uL Continue pressing Enter counting each key press until a tiny drop of liquid appears at the end of the needle seal line After each key press wait until the sound of the syringe motor stops before pressing Enter again Record the number of times you pressed Enter including the first time in Step 17 Also notice the size of the liquid drop This will be compared to the size of the liquid drop in Step 32 Select the following options ASPIRATE 60 uL from NEEDLE SYRINGE SPEED 3 ACTION ASPIRATE Press Enter 5 24 Doc 031935 01 4 04 5 Service 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Select VIAL FLU and press Enter The needle arm moves to the flush port Select the fo
31. Start a schedule or continue a schedule that is waiting for a run command Turn the optional tray temperature control on and off e Turn the optional column temperature control on and off Note this option is currently not available for the AS50 USB See Appendix D for details about TTL control Doc 031935 01 4 04 2 Description 2 5 1 Local Direct Control Mode You can directly control the following AS50 functions from the front panel TTL and relay outputs Injection valve position Injection valve 2 position if installed Tray set point if installed Set the above functions from the DETAIL STATUS screen see Figure 2 9 DETAIL IDLE VIAL sf TIME min Trib INJ VOl uL URAY 2 mE INIEY 11 251202 RLY 2 COL C INJ VLV LOCAL DIRECT CONTROL GSH Help Prompt 2 5 2 Figure 2 9 Detail Status Screen Example NOTE The CS VLV field sets the position of a column switching valve This valve option is used in chromatography and thermal compartments which are not available currently for the AS50 USB Local Schedule Control Mode The AS50 runs all injections according to a programmed schedule The schedule defines a series of vials to be sampled and the operating parameters to run on each vial A schedule consists of the following parameters The vials to sample The number of injections to draw from each vial The volume of each injection T
32. Using a flathead screwdriver unscrew and remove the syringe drive connecting screw see Figure 5 1 Hold the syringe at the top and bottom and unscrew it from the valve Remove the white Teflon washer from the syringe valve port Note To remove the washer push a piece of 0 8 mm 0 031 in OD PEEK tubing into the center of the washer and twist sideways to dislodge the washer Syringe Drive Connecting Screw Remove Install a new white Teflon washer P N 061315 into the new syringe Figure 5 1 Removing the Syringe Filling the New Syringe and Removing Bubbles 1 Fill a container with isopropyl alcohol Place the threaded end of the syringe into the container and slowly pull liquid into the syringe Remove the syringe from the liquid and point the threaded end up into the air If air bubbles are present push the syringe plunger up and pull down rapidly with short movements to dislodge the bubbles Repeat until all bubbles are dislodged Place the threaded end of the syringe into the isopropyl alcohol again and draw a full syringe volume up by slowly pulling out the plunger Keep the syringe in the liquid and slowly push the syringe plunger until the bubbles exit the syringe Then fill the syringe completely 5 4 Doc 031935 01 4 04 5 Service 5 Remove the syringe from the liquid While holding it vertically push a small amount of liquid out the top
33. completely Make sure the magnet on the inside lower front edge of the door is present Also make sure the magnet on the lower edge of the autosampler compartment is present If a magnet is missing contact Dionex Doc 031935 01 4 04 4 13 AutoSelect AS50 Autosampler 443 Leak detected in tray syringe area Possible Causes Excess liquid in the waste container is preventing liquid from siphoning through the waste tubing Blocked or crimped waste tubing Leaking inject port Miscalibrated leak sensor Actions Empty the waste container Make sure the waste tubing is not crimped and that it is not pushed against the bottom of the waste container e If the inject port is leaking see Section 4 2 3 e Ifthe tray is dry but the error is still appearing recalibrate the sensor see Section 5 10 4 14 Doc 031935 01 4 04 4 Troubleshooting 445 446 e 447 448 449 Invalid leak sensor reading May be open or short circuit or bad sensor See Leak Sensor Calibration screen for more information Actions The leak sensor card the cables or the sensor must be replaced Contact Dionex Current leak sensor voltage is outside of legal calibration range Attempting to calibrate when the leak sensor voltage reading is outside the normal dry range Possible Causes The sensor is not dry enough for calibration Actions Clean and dry the area thoroughly and reca
34. 04 A Specifications A 1 Autosampler A 1 1 Electrical Main Power 100 to 240 Vac 50 60 Hz 6 amps The AS50 power supply is main voltage auto sensing and requires no adjustment Fuses Two 3 15 amp fast blow 127 fuses P N 954745 1 2 Environmental Physical Ambient Operating 10 to 40 C 50 to 104 F Temperature Operating Humidity 10 to 95 relative humidity non condensing Decibel Level lt 60 db at A WEIGHTING setting Dimensions 58 cm high x 30 cm wide x 46 cm deep 23 in x 12 in x 19 in Weight lt 30 kg lt 65 Ib Doc 031935 01 4 04 1 AutoSelect AS50 Autosampler A 1 3 Valves Optional Injection Two two position six port electrically activated Rheodyne valves simultaneous PEEK with Tefzel rotor seal injection option only A 1 4 Injection Injections Per Vial 1 to 99 Vial Sizes Available 0 3 mL polymer vials P N 055428 package of 100 1 5 mL glass vials P N 055427 package of 100 10 mL PolyVials P N 055058 package of 100 Variable Volume 1 9 to 99 9 uL in 0 1 uL increments Range 100 to 1000 uL in 1 uL increments If the simultaneous injection option is installed 1000 to 5000 uL 5 mL sample syringe 1000 to 8000 uL 10 mL sample syringe Injection Loop Size 25 uL standard other sizes are available simultaneous injection option only A 2 Doc 031935 01 4 04 A Specifications A 2 Sample Temperature Control Unit Optional Temperature C
35. 1 on a pump a signal from the AS50 will turn the pump motor on and off In the TTL and RLY fields on the TIMED EVENTS screen select 1 on or 0 off See Appendix D for details about TTL and relay control Editing a Method After creating a method you can modify it by changing adding or deleting steps and parameters These changes can be made at any time If the method you are editing is currently running changes made to steps that have not yet been completed are performed in the current run Changes made to steps that have already been completed are performed the next time the method runs 1 Goto the METHOD MENU screen press Menu and 3 2 Inthe EDIT field enter the number of the method to be edited 3 Goto the method screen to be changed and edit the parameters as required 4 Savethe method see Section 3 10 2 The changes can be saved to the same method number or to a new unused method number If you save to a new number the original method remains unchanged Deleting a Method 1 Goto the METHOD MENU screen press Menu and 3 2 Position the cursor in the EDIT field and press Delete A confirmation message appears 3 Press Delete again to delete the method Doc 031935 01 4 04 3 31 AutoSelect AS50 Autosampler 3 10 8 Copying a Method 1 2 Go to the METHOD MENU screen press Menu and 3 Position the cursor in the EDIT field and enter the number of the existing method to be copied
36. 127 mm 0 005 in 5 20 Doc 031935 01 4 04 5 Service 10 11 12 13 14 15 16 17 18 19 20 2 Verify that the needle is centered over the inject port opening Adjust the alignment by changing the X ADDRESS and or Y ADDRESS values Change the values in 2 to 5 step increments After each value change press Enter The needle arm moves up and then adjusts to the new X or Y position To move the needle to the right increase the X ADDRESS value To move the needle to the left decrease the X ADDRESS value To move the needle forward increase the Y ADDRESS value To move the needle back decrease the Y ADDRESS value After each adjustment move the cursor to the Z ADDRESS field keep the value at 180 and press Enter The needle arm moves back down Observe the alignment and adjust the position again if needed When the adjustments are complete press Home The needle arm moves to the home position Press Enter Position the cursor in the X or Y ADDRESS field and press Enter The needle arm moves to the selected X Y position Recheck the alignment and adjust the X and or Y position if necessary Position the cursor in the Z ADDRESS field Enter the following value in the Z ADDRESS field 230 Press Enter The needle moves down into the inject port Press Home Press Menu three times to return to the MENU of SCREENS Press 8 to go to the DIAGNOSTICS MENU and press 5 to go t
37. 2 00 3 00 Figure 2 11 Example Chromeleon System Control Panel To control AS50 operation click the Sampler Settings button to open the Injector Settings window This window lets you select various AS50 operating parameters including the vial to be sampled the injection 2 20 Doc 031935 01 4 04 2 Description volume and the position of the injection valve You can also initiate a flush or prime operation from this window Injector Settings Test AMAR x AutoSampler Settings Connected B BHSE 1 Pos 1 gt Load Inject Injection Valve Vol 20 0 pl Inj Wait Flush Volume 100 pl 3 Flush Prime Volume 150 pl 3 Prime Prime Syringe Sample 3 Syringe Speed Prime Reservoir Reservoir A Idle Close Figure 2 12 Example Chromeleon System Control Panel Injector Settings Window Doc 031935 01 4 04 2 21 AutoSelect AS50 Autosampler 2 6 Theory of Operation 2 6 1 Understanding the Wait Operation NOTE This section pertains to AS50 operation in Local Schedule Control mode only The wait operation is a step in the sample prep phase of a method After performing all other sample prep steps the AS50 waits for a continue command before performing the method s timed events The continue command can be from either the front panel Hold Run button or a TTL input signal The wait
38. 2 2 Connecting to Valves Installed in the AS50O E 5 Specifying the Plumbing Configuration E 7 Controlling the Injection Valves from the AS50 Front Panel E 8 E 4 1 Controlling the Injection Valves Directly E 8 E 4 2 Controlling the Injection Valves in a Schedule E 9 viii Doc 031935 01 4 04 Contents E 5 Setting Up Chromeleon E 10 E 5 1 Assigning Unique Names to Devices E 10 E 5 2 Creating Sequences Files and QNT Files E 13 F Unpacking Instructions ix Doc 031935 01 4 04 AutoSelect AS50 Autosampler X Doc 031935 01 4 04 1 1 1 Introduction Overview The Dionex AutoSelect AS50 Autosampler precisely delivers from 1 0 to 99 9 uL in 0 1 uL increments or 100 to 1000 uL in 1 uL increments of sample to an injection valve If the AS50 is equipped with the simultaneous injection option the AS50 delivers sample to two injection valves in two independent ion chromatography IC systems With the simultaneous injection option the AS50 delivers from 1000 uL to 5000 uL of sample if the 5 mL syringe is installed or up to 8000 uL if the 10 mL syringe is installed The sample is delivered equally to the two IC systems half of the volume to each Two types of trays are available for sample vials one for 1 5 mL vials and one for 10 mL vials During sampling
39. 5 mm 1 2 in from the end being careful not to restrict the inner diameter 7 Pushthe end of the green AS50 TRAY line into the small round opening on the right side of the AS50 tray see Figure B 5 Make sure the injection valve waste line is not elevated above the injection valve at any point between the valve and the AS50 drip tray NOTE Do not push the end of the waste line into the large drain tube opening in AS50 tray Doc 031935 01 4 04 B 9 AutoSelect AS50 Autosampler B 3 5 Connecting the AS50 to the Chromeleon PC Optional The AS50 rear panel see Figure B 3 provides a USB receptacle for connecting 550 to a USB port on the Chromeleon PC or a USB hub Before connecting the USB cable and turning on the AS50 power verify that Chromeleon is installed on the PC When Chromeleon is installed first the USB driver information is loaded when the power is turned on Chromeleon then automatically detects the new USB device Refer to Installing the Chromeleon Chromatography Management System with a _ Dionex lon Chromatograph Document No 031883 for installation instructions and for details about USB connections 1 Locate the USB cable P N 960777 in the AS50 Ship Kit P N 061270 or P N 061271 Connect the cable between the AS50 and computer as shown in Figure B 8 USB Cable Computer Running Chromeleon Plug the USB cable s B connector into the USB Plug the USB cable s A
40. 80 of the volume in the vial The sample syringe is flushed with a volume equal to the amount of concentrate plus one full syringe stroke Volume Vial Volume Reservoir Vial DILUTE 12 3 uL S 1 H234uL A 3SMP Concentrate Concentrate Vial Diluent Diluent Destination Vial Volume Volume Reservoir 1 0 to 99 9 uL 1to 100 aluminum tray 100 to RES A B 1 to 100 aluminum tray 100 1000 uL 1 to 49 plastic tray 9999 mL C D 1 to 49 plastic tray SMP S 1 S 9 SMP S 1 S 9 Concentrate Concentrate Diluent Diluent Destination Doc 031935 01 4 04 3 25 AutoSelect AS50 Autosampler 3 10 4 Selecting Method Setup Parameters To go to the METHOD SETUP screen 1 Go to the METHOD MENU 2 Position the cursor on the 2 field SETUP 3 Press Enter Figure 3 15 shows the default setup parameters METHOD SETUP METHOD 25 CYCLES IME min SAMPLE NEEDLE HEIGHT TRAY TEMPERATURE COLUMN TEMPERATURE WAIT FOR TEMP STABLE Help Prompt Figure 3 15 Method Setup Screen Default The table below shows the value ranges for each parameter See the following section for details about each parameter Parameter Value Range Default Cycle Time 1 to 240 minutes Off Sample Needle Height 0 to 46 mm 2 mm Tray Temperature 4 to 60 C Off optional 20 C when on Wait For Temp Stable Yes No No Sample needle height is measured from the bottom of the vial Zero mm is closest to the bottom
41. 9 3 18 3 23 C 11 Modes of operation See Local mode See Remote mode Module setup B 14 Module Setup Menu C 19 Front Panel screen C 20 Inject Port Alignment screen C 26 Installed Options screen C 19 Plumbing Configuration screen C 21 System Parameters screen C 24 Time Date screen C 23 Moduleware revision level C 31 Motion command to DSP was aborted 4 15 Moving the AS50 B 2 Multi level calibration standards 2 10 N Needle Flushing during a method 3 24 Replacing 5 8 Washing the outside 2 30 Needle arm 2 10 Manually controlling C 37 Needle height 2 10 3 18 C 10 Specifying in a method 3 22 3 27 Needle seal assembly Leaking 4 22 Part numbers 5 2 Replacing 5 17 No injection 3 34 Non recoverable error Both internal and external inject valves are connected 4 19 Non recoverable motion error occurred 4 9 Normal edge TTL input control Definition D 3 Selecting C 30 D 3 Normal pulse TTL input control Definition D 4 Selecting C 30 Numeric buttons 2 5 O Opening the door 2 4 3 12 Operating humidity A 1 Operating modes 2 16 Local 2 16 Remote 2 19 Selecting C 5 Operation 3 1 Default parameters 3 6 Filling vials 3 1 Getting ready 3 1 Power up 3 3 Running a schedule of injections 3 8 Selecting the control mode 3 7 Operation could not be completed because tray is not installed 4 6 Operational screens C 1 Options Injection valve E 2 Installed Options screen C 19 Sam
42. AutoSelect AS50 Autosampler D 2 1 Example TTL Relay Connections The following example connections can be used to connect AS50 relay and TTL outputs to TTL inputs on an ICS 1000 1500 2000 system These connections allow the 550 to control the following functions Injection valve position e Pump flow Detector autozero NOTE Refer to the ICS 1000 1500 2000 operator s manual for an example of how to set up the AS50 and the IC system for stand alone operation operation without Chromeleon software ICS 1000 1500 2000 Rear Panel Connections AS50 Rear Panel Connections TTL 2 OUT TTL 1 IN TTL 1 OUT TTL 2 Ni TTL 3 NU RLY 2 OUT Tus rm RLY 1 T uoi 7 8 9 10 11 12 Tale a 9 9 o e o a 412 pos unu 4122 Pump On Off TTL GND pos 11 12 12 Figure D 5 Example TTL Relay Connections ICS 1000 1500 2000 D 6 Doc 031935 01 4 04 E Simultaneous Injections When the AS50 is equipped with the simultaneous injection option a single AS50 can deliver sample to two independent ion chromatography IC systems The IC systems do not need to be the same type The simultaneous injection option includes the following components e A5mLor 10 mL sample syringe Optional Two injection valves installed in the AS50 autosampler compartment If the AS50 does not have two injection valves connection to injection valves in two ICS 1000 1500 2000 systems is required Optional C
43. Communication problem between the DSP and the CPU Actions Turn the power off and then back on Ifthe error message appears again contact Dionex Method does not exist The schedule contains a method that does not exist Possible Causes Incorrect method number entered into the schedule Actions Enter the method number of an existing method in the schedule Create a new method and save it to the method number indicated in the schedule Doc 031935 01 4 04 4 Troubleshooting 348 A failure has occurred during power up The status of all tests run during power up are indicated on the Diagnostic Tests screen which follows this error message At power up AS50 electronics are tested If one or more tests fails this message appears On the DIAGNOSTIC TESTS screen an indicates that a test failed and a P indicates it passed Possible Causes One or more of these tests has failed indicating a problem with that portion of the electronics Actions e See Section C 9 2 for a description of each test Ifthe SYRINGE COMM test failed make sure the sample syringe cable is installed and connected to the correct connector on the AS50 rear panel see Section B 3 3 If the prep syringe is installed check its cable connection also Rerun the test e Ifthe test still fails contact Dionex 375 Anattempt was made to use the sample prep syringe This option is not installed Two of
44. Eres B 14 B 3 9 Configuring the AS50 in Chromeleon B 17 B 3 10 1 1 B 18 B 3 11 Removing Bubbles from the B 21 vi Doc 031935 01 4 04 Contents C Display Screens 1 Menu OF Screens aga eat vee C 2 C 2 Main Status Screen eee C 3 C 3 Detail Status Screens ceceo 6 4 Method Menu iioc Ob ue awe Se et ened es C 9 C 4 1 Sample Prep C 10 C 4 2 Method Setup C 13 C 4 3 Timed Events C 15 C 5 Schedule Screen oo ss pee e eme pes e rU C 17 C 6 Module Setup Menu C 19 C 6 1 Installed Options C 19 C 6 2 Front Panel C 20 C 6 3 Plumbing Configuration Screen C 21 C 64 Time Date Screen s eri eon i o a C 23 C 6 5 System Parameters Screen C 24 C 6 6 Inject Port Alignment Screen C 26 C 6 7 Door Interlock Bypass C 27 Flush Prime Screen 28 8 Time Function In Screen C
45. Figure 2 4 MENU of SCREENS MAIN STATUS 6 FLUSH PRIME DETAIL STATUS 7 TIME FUNCTION IN METHOD MENU 8 DIAGNOSTIC MENU SCHEDULE 9 PRINT MENU MODULE SETUP MENU 10 TIME FUNCTION OUT Help Prompt Figure 2 4 Menu of Screens To select a screen from the menu Press the keypad number button corresponding to the screen s number on the menu or Move the cursor to highlight the screen number and press Enter To display a brief description of each screen Press the Help button See Appendix C for details about each screen To edit a field on the screen 1 Press a cursor arrow button to position the cursor in the field to be edited Fields on the screen that are in reverse video blue letters on white background can be edited Other fields display information only 2 Ifthe field accepts numerical values press the desired numerical buttons to enter the value If the field has predetermined options press the Select A or Select V button to choose the desired option 3 To confirm the selected value press Enter or move the cursor out of the field by pressing an arrow button Doc 031935 01 4 04 2 7 AutoSelect AS50 Autosampler 2 3 Autosampler Compartment Reagent Reservoir Optional Prep Syringe Optional e Sample Syringe Sampling Needle Arm Flush Waste Sample Vials and Tray Figure 2 5 Autosampler Compartment Features 2 8
46. InLocal mode the AS50 receives commands from the front panel buttons and screens When the AS50 is powered up it is always in Local mode In Local mode all 550 operating functions are available In Locked Remote mode Chromeleon software sends commands from the host computer via the USB interface Connecting the AS50 to a Chromeleon Control panel immediately selects the Locked Remote mode Disconnecting from Chromeleon returns the AS50 to Local mode The control mode determines when operating commands are executed e In Direct control the 550 executes commands immediately In Schedule control the AS50 executes commands by running a programmed schedule of injections Schedule control is used in Local mode only The table below summarizes the AS50 operating and control mode configurations Operating Control Mode Autosampler Operation Locked Remote Direct Control Commands are sent from Chromeleon and executed immediately when received Local Direct Control Commands are entered from the AS50 front panel and executed immediately after being entered Local Schedule Control Commands are entered from the AS50 front panel and executed by running a schedule programmed from the front panel TTL input signals from a remote controller such as an integrator can also be used to control the AS50 The AS50 accepts TTL signals when it is in Local or Locked Remote mode The following functions can be controlled via TTL input signals
47. LOAD INJECT If the AS50 is not connected to Chromeleon a TTL ENABLED VALVE TTL1 connection is required between the AS50 TTL DISABLED TTL2 output and a TTL input on the IC system This will default allow you to control the injection valve in the IC TTL1 system from the ASSO front panel See Appendix D ENABLED for TTL connection instructions C 46 Doc 031935 01 4 04 D TTL and Relay Control D 1 TTL and Relay Connections A 12 pin connector strip for TTL relay control is located on the AS50 rear panel The connector provides two relay outputs two TTL outputs and four TTL inputs see Figure D 1 Pin Function RELAY TTL OUT TTL IN TTL GND OUT 1 1 2 1 2 1 2 3 4 1 2 Connector Position 1 2 3 4 56 7 8 9 10 11 12 1 7 EE Solid State Relay Contacts Output 3 aae Solid State Relay Contacts Output 5 TTL Output 1 1 kO pull up to 5 100 mA sink 6 TTL Output 2 1 kO pull up to 5 100 mA sink 7 TTL Input 1 unassigned 8 TTL Input 2 Start Continue Schedule 9 TTL Input 3 Tray Temperature ON OFF 10 TTL Input 4 Thermal Compartment ON OFF 11 Ground 12 Ground Figure D 1 TTL and Relay Connector on Rear Panel Relay loads in excess of 200 mA or with included power supplies over 60 V may damage the relay drivers on the CPU CAUTION Doc 031935 01 4 04 D 1 AutoSelect AS50 Autosampler The outputs can be used to c
48. Position the cursor in the SAVE TO field and enter a new unused method number Press Enter The parameters from the original method are copied to the new method and the original method is unchanged 3 10 9 Example Method NOTE The sample preparation option is required for this method This method uses the sample preparation option to dilute a sample before injection In this method the 550 delivers 600 uL of the concentrated sample from vial 6 and 5400 uL of diluent from reagent reservoir A to the sample vial Default values are used for all other method parameters Figure 3 17 shows the sample prep steps for the method SAMPLE PREP METHOD 1 DILUTE 600uL 6 5400uL A SMP WAIT Help Prompt Figure 3 17 Dilution Method Example Sample Prep Steps 3 32 Doc 031935 01 4 04 3 Operation and Maintenance 3 11 Creating Schedules from the AS50 Front Panel The schedule defines injection parameters for each vial to be sampled A schedule consists of the following parameters The vials to sample The number of injections to draw from each vial The volume of each injection The method to run on each injection The action to take when a scheduled vial is missing You can create a new schedule or edit an existing schedule when the AS50 is running on hold or stopped If the schedule you are editing is currently running changes to steps that have not yet been completed are performed in the current run Changes
49. Remote Direct Control mode In this mode you cannot change any operating parameters from the front panel keyboard To return the AS50 to Local mode disconnect the AS50 from the Chromeleon Control panel or turn off 550 power Two modes of software control are available With direct control you select operating parameters and commands from the Chromeleon menu bar toolbars and Control panels With automated control you create a list of samples a sequence to be processed automatically The sequence includes programs with commands and parameters for controlling the AS50 acquiring sample data and producing reports Control Panels NOTE This section provides a brief overview of how to open and connect to a Chromeleon Control panel For details refer to the Chromeleon Help or user s manual For instructions on how to configure the AS50 in Chromeleon see Section B 3 9 Chromeleon provides two types of Control panels for the AS50 e System Control panels are used for controlling and monitoring 550 operation System Control panels are described below The Wellness panel is used to test and calibrate the leak sensor and diagnostic functions The Wellness panel is described in Section 5 15 System Control panels are used for controlling and monitoring system operation including AS50 operation Control panels are located in the Dionex Templates Panels Dionex IC folder in the Chromeleon Browser Chromeleon provides pane
50. Select A or Select V to cycle through a list of relative vial positions SMP the current sample vial S 1 one position past the sample vial or S 9 9 positions past the sample vial The destination vial list also includes a WST waste option Mixes the vial contents by repeatedly drawing and expelling a volume of sample Mixing is done with the prep syringe if it is installed otherwise the sample syringe is used Enter the position of the vial to be mixed see above default SMP X Enter the number of times to repeat the mixing operation from 1 to 99 default 10 uL Enter the volume to be drawn and expelled from 1 0 to 9999 uL The default is 500 uL if the prep syringe is installed otherwise it is 250 uL The mix volume must be equal to or less than the syringe volume Flushes the needle with a specified volume from 1 to 999 uL default 250 uL The sample syringe and flush reservoir are always used for flushing If the volume specified is greater than the syringe volume the syringe takes multiple strokes to achieve the volume This operation is independent from the flush that takes place before each injection in a schedule see Section 2 6 6 Moves a specified volume of reagent from a reservoir to a vial using the prep syringe DISPENSE is available only if the sample preparation option is installed uL Enter the volume to dispense from 100 to 9999 uL default 1000 uL source Select the reservoir fro
51. a vial is present in the scheduled position in the tray If the vial is missing the AS50 can either stop and wait or skip the vial and continue The action is specified in the schedule 1 In the SCHEDULE screen move the cursor to the MISSING VIAL ACTION field and select either STOP or SKIP STOP The 550 stops when a scheduled vial is missing allowing you to place a vial in position To resume the schedule press Hold Run SKIP The AS50 ignores a missing vial and continues to the next vial 2 Press Enter or a cursor arrow to confirm the choice 3 34 Doc 031935 01 4 04 3 Operation and Maintenance 3 11 4 Defining Schedule Lines Each schedule ine contains operating parameters for one vial Line numbers are automatically generated when you define parameters for the vials in the schedule If the parameters are different for each vial line numbers are shown consecutively If you define identical parameters for a series of vials line numbers are shown in a range format see Figure 3 19 A schedule can include up to 999 lines Each range of vials or one horizontal line on the screen is one schedule step see Figure 3 19 A schedule can have up to 203 steps VIAL INJ INJ LINE START END VIAL VOL uL METHOD Step 14 1 1 1 1 25 0 1 Step2 2 11 5 14 3 12 3 12 Line Numbers in Range Format Figure 3 19 Schedule Screen Example Inserting Steps Position the cursor in the LINE field below whe
52. hazardous situation which if not avoided could result in death or serious injury Indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury Also used to identify a situation or practice that may seriously damage the instrument but will not cause injury Indicates that the function or process of the instrument may be impaired Operation does not constitute a hazard Messages d avertissement en francais Signale une situation de danger imm diat qui si elle n est pas vit e entrainera des blessures graves mortelles Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entrainer des blessures graves mortelles Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entrainer des blessures mineures mod r es galement utilis pour signaler une situation ou une pratique qui pourrait gravement endommager l instrument mais qui n entrainera pas de blessures Doc 031935 01 4 04 1 3 AutoSelect AS50 Autosampler Warnhinweise in Deutsch Bedeutet unmittelbare Gefahr MiBachtung kann zum Tod oder schwerwiegenden Verletzungen f hren ACHTUNG Bedeutet eine m gliche Gef hrdung MiBachtung kann zum Tod oder schwerwiegenden Verletzungen f hren WARNUNG Bedeutet eine m gliche Gef hrdung MiBachtung kann zu kleineren oder mittelschweren Verletzungen f hren Wird auch verwendet wenn eine Situation
53. made to steps that have already completed are performed the next time the schedule runs 3 11 1 Creating a New Schedule 1 Go to the SCHEDULE screen If you are opening the screen for the first time after the power is turned on the screen displays an empty unused schedule see Figure 3 18 Otherwise the screen displays the last schedule that was edited SCHEDULE EDIT SAVE TO RUN MISSING VIAL ACTION STOP VIAL INJ INJ LINE START END VIAL VOL uL METHOD 1 1 Bi 1 25 0 1 Help Prompt Figure 3 18 Schedule Screen Default Settings 2 Ifaschedule is currently displayed position the cursor in the EDIT field Enter the number of an unused schedule or press Select A or Select V to cycle through the choices 1 through 9 Doc 031935 01 4 04 3 33 AutoSelect AS50 Autosampler 3 Press Enter 4 Select an action to take if a vial is missing see Section 3 11 3 5 Define the schedule lines required for your sample injections see Section 3 11 4 NOTE If injections per vial is zero for a particular vial only the sample prep phase of the method is run 6 Save the schedule 3 11 2 Saving a Schedule 1 Position the cursor in the SAVE TO field 2 Do one of the following To save the current schedule press Enter e save to a different schedule enter a new number and then press Enter 3 11 3 Selecting an Action if a Scheduled Vial Is Missing Before beginning each injection the AS50 checks that
54. or 0 3 mL 10 mL plastic 49 Poly Vials Possible Causes The vial number specified in the method sample prep steps or the schedule steps is not available for the type of tray installed in the autosampler compartment Actions e Use a tray type that accommodates the specified vial Change the vial specified in the method or schedule 388 ANAK command was received from the DSP The CPU sent a command to the DSP which the DSP cannot execute Possible Causes An installed option is not being recognized Actions Check the INSTALLED OPTIONS screen see Section C 6 1 to verify that all optional features are being recognized A check mark should appear beside each installed option e If an installed option is not checked turn off the power and then turn on the power and recheck the INSTALLED OPTIONS screen If the problem persists contact Dionex Doc 031935 01 4 04 4 7 AutoSelect AS50 Autosampler 389 390 391 Timed out waiting for motion complete command from DSP The DSP could not complete a motion in the time allowed Possible Causes Mechanical problem with the autosampler needle arm e valve is not being actuated Actions Check for an obstruction in the autosampler compartment Manually test the autosampler needle arm movement using the XYZ TEST screen see Section C 9 3 Manually test the sample syringe the sample prep syringe and the injection valve s using the LIQUID
55. screen press Menu 5 and 5 2 Position the cursor in the SYRINGE SPEED field 3 Press Select A or Select V to select the desired speed or enter the speed by pressing a numeric button 1 5 1 is the slowest speed and 5 is the fastest The default speed is 3 Select a slower setting for more viscous samples The syringe speed selected on the SYSTEMS PARAMETERS screen is used for the following operations e Aspirating and dispensing sample during loop loading Aspirating and dispensing sample during sample prep operations except for mixing and dilution Aspirating from and dispensing to the flush reservoir during sample prep operations and loop loading Aspirating an air bubble Factory set speeds are used for the following operations Aspirating and dispensing sample during mixing e Aspirating and dispensing concentrate during dilution Aspirating and dispensing reagent e Flushing Doc 031935 01 4 04 3 45 AutoSelect AS50 Autosampler 3 14 Routine Maintenance This section describes routine maintenance procedures that the user can perform Any other maintenance procedures must be performed by Dionex personnel 3 14 1 Daily A CAUTION AN MISE EN GARDE VORSICHT 3 14 2 Check for air bubbles in the syringe s and tubing and remove them if they appear see Section B 3 11 Check the volume of liquid in the flush bottle and reagent bottle s if installed and refill when needed After
56. screen to the software If the selected size is 5000 or 10000 uL simultaneous injection option functions are enabled in Chromeleon Doc 031935 01 4 04 E 7 AutoSelect AS50 Autosampler E 4 Controlling the Injection Valves from the AS50 Front Panel In Local mode you can control the injection valves either directly or in a schedule NOTE See Section E 5 for how to use Chromeleon to control the valves E 4 1 Controlling the Injection Valves Directly To change the position of the injection valves when a schedule is not running 1 Gotothe DETAIL STATUS screen press Menu and 2 see Figure E 8 2 Move the cursor to the INJ VLV 2 or INJ VLV 1 field press Select A or Select V to toggle the position and press Enter DETAIL IDSE VIAL DUME i INJ VOL 12 TRAY NIE dL 25 20 LOOP 100 25 uL 2 GO Paar C VLV 2 LOCAL DIRECT CONTROL INJ 1 Help Prompt Figure E 8 Detail Status Screen Simultaneous Injection Mode E 8 Doc 031935 01 4 04 E Simultaneous Injections E 4 2 Controlling the Injection Valves in a Schedule To change the positions of the injection valves during a schedule run set the positions on the method TIMED EVENTS screen By default both valves are in the Load position at INIT and in the Inject position at time zero see Figure E 9 INIT LOAD LOAD 0 00 INJECT INJECT Help Prompt Figure E 9 Timed Events Screen Sim
57. starts the Action On Doc 031935 01 4 04 D 3 AutoSelect AS50 Autosampler schedule if it is not currently running or continues the current schedule if it is on hold The action of the positive rising edge depends on the function For the START CONTINUE function the rising edge has no effect For the TRAY TEMP ON OFF and COLUMN TEMP ON OFF functions the rising edge turns off sample temperature control and column temperature control Note The column temperature control option is not currently available for the AS50 USB Inverted Edge The inverted Action On edge mode works identically to ee AM the normal edge mode except INVERTED that the positive and negative FOE a ond is edges are reversed in function Action Off T No Effect Normal Pulse In normal pulse operation the negative falling TTL 5 V edge of the TTL signal is the ree ay active edge and the positive ae Action Action Off rising edge is ignored dd A pulse width of 50 ms or more is guaranteed to be detected A pulse width of 4 ms or less is guaranteed to be ignored The action for pulse widths that are greater than 4 ms and less than 50 ms is undefined Inverted Pulse The inverted pulse mode operates identically to the normal pulse mode except that the positive and negative edges are reversed in function Action On _Action Off Y INVERTED D 4 Doc 031935 01 4 04 D T
58. the Chromeleon Wellness panel see Section 5 15 If you are calibrating an existing not a new leak sensor remove the drip tray from the autosampler see Section 5 4 and thoroughly clean and dry the tray and sensor Replace the sensor in the tray and reinstall the drip tray see Section 5 5 Press Menu 8 and 0 to go to the LEAK SENSOR CALIBRATION AND STATUS screen Press Select A or Select V to select CAL and then press Enter The current measured value becomes the new calibration value Doc 031935 01 4 04 5 27 AutoSelect AS50 Autosampler 5 11 Removing the Sample or Prep Syringe Valve 1 From the MENU of SCREENS press 5 and 3 to go to the PLUMBING CONFIGURATION screen 2 Move the cursor to the SAMPLE SYRINGE CONFIG field or the PREP SYRINGE CONFIG field depending on which syringe requires initialization 3 Press Select A to select the INITIALIZE option Valve Screws 4 Press Enter Remove The syringe plunger moves to the initial position and the syringe motor shuts off 5 Unscrew and remove the syringe drive connecting screw and the two screws on the syringe valve see Figure 5 21 6 Pull the valve and syringe assembly straight out to remove it Syringe Drive Connecting Screw Remove 7 Holding the syringe at the top fitting unscrew it from the valve 8 Set the syringe aside in a safe place
59. the METHOD SETUP screen to set the initial conditions and default parameters for a method METHOD SETUP SAMPLE NEEDLE HEIGHT SAMPLE TRAY TEMPERATURE COLUMN TEMPERATURE WAIT FOR TEMP STABLE Help Prompt METHOD 25 123 min 46 mm 20 C eG YES Screen Field CYCLE TIME SAMPLE NEEDLE HEIGHT Figure C 7 Method Setup Screen Description Values The time between injections When a method is 1 to 240 assigned a cycle time its timed events are not minutes executed until the specified time has elapsed See default off Section 2 6 5 for details Cycle time is ignored in the following situations If WAIT FOR TEMP STABLE is enabled and the tray or column temperature has not stabilized by the end of the cycle time Ifthe wait step of the method s sample prep phase is enabled and a continue command has not occurred by the end of the cycle time Controls the height of the sample needle above 0 to 46 mm the bottom of the vial default 2 NOTE For 0 3 mL and 1 5 mL vials use a maximum needle height of 2 mm to ensure the vent in the needle is positioned inside the vial Doc 031935 01 4 04 C 13 AutoSelect AS50 Autosampler Screen Field SAMPLE TRAY TEMPERATURE COLUMN TEMPERATURE WAIT FOR TEMP STABLE Description The sample tray temperature set point Pressing Select A or Select V toggles the tray temperature control on and off Available only when
60. the injection valve E 2 Loading the loop 3 30 Loading the tray 3 1 Local mode 2 16 Selecting 2 16 3 7 C 5 Locked Remote mode 2 16 Functions performed by Chromeleon 2 19 Selecting 3 7 Logs Menu screen C 39 Loop loading 3 30 Loop size B 14 C 7 C 21 For simultaneous injections E 7 Loop volume 3 38 M Main Status screen 2 6 C 3 Maintenance 3 46 Annual 3 47 Periodic 3 46 Manual control of syringes valves and needle arm C 37 Maximum number of steps reached 4 1 Memory is full 4 2 Menu button 2 5 Menu of Screens 2 7 C 2 Selecting screens from 3 5 Menu structure chart 3 4 C 1 Message Log screen C 40 Messages See Error messages Method does not exist 4 2 Method Menu C 9 Method Setup C 13 Sample Prep screen C 10 Timed Events screen C 15 Method Setup screen 3 26 C 13 Method Schedule database has been corrupted 4 13 Methods Controlling the injection valve 3 30 Controlling TTL and relay outputs 3 31 Copying 3 32 Creating 3 16 Defining sample prep steps 3 18 3 19 Defining the INIT step 3 29 Definition of 3 16 Deleting 3 31 Index 6 Doc 031935 01 4 04 Index Description of 2 18 Editing 3 31 Example 3 32 Saving 3 17 Selecting sample prep parameters 3 18 C 10 Selecting setup parameters 3 26 C 13 Selecting timed event parameters C 15 Specifying in a schedule 3 36 Specifying the needle height 3 22 3 27 Missing vial action 3 34 C 17 Mixing vials 2
61. the injection valve One cut segment volume 20 uL passes to waste and the next 50 uL is loaded into the loop At injection 50 uL is injected After injection the remaining cut segment volume 20 uL flows to waste Doc 031935 01 4 04 3 43 AutoSelect AS50 Autosampler 3 12 3 Partial Loop Limited Sample Injections When performing a partial loop limited sample injection the AS50 draws only the volume to be injected from the sample vial see Figure 3 24 NOTE If the 550 is set up for simultaneous injections only full loop injections can be performed Sample Y Syringe Y B I Inject Port Waste Waste Waste Waste WY R JF ar Pump Column INJECT INJECT INJECT LOAD 1 Draw air 2 Draw sample 3 Draw air again to 4 Deliver sample cushion the sample to the loop KEY Deionized Water wm Air mm Eluent wm Sample Figure 3 24 Partial Loop Limited Sample Injection Sequence 3 44 Doc 031935 01 4 04 3 Operation and Maintenance Setting Up a Partial Loop Limited Sample Injection 1 When defining injection parameters on the SCHEDULE screen enter a sample injection volume that is less than the volume of the sample loop 2 the SYSTEM PARAMETERS screen enter a CUT SEGMENT VOLUME of 0 3 13 Adjusting the Sample Syringe Speed for Different Sample Viscosities 1 Go to the SYSTEM PARAMETERS
62. the method sample prep operations DISPENSE and DILUTE can be used only when the sample preparation option is installed See Section 3 10 3 for details Possible Causes A DISPENSE or DILUTE operation was specified in the sample prep steps of the current method although the sample preparation option is not installed Actions Remove the step s requiring the sample preparation option Select a method that does not require the sample preparation option Doc 031935 01 4 04 4 3 AutoSelect AS50 Autosampler 376 377 Time out occurred waiting for syringe to complete an action Every syringe action is assigned a maximum time in which the action should be completed The syringe did not complete its task in the allotted time indicating either a mechanical or a communication problem Possible Causes Faulty syringe Faulty cable Actions Check the syringe motion If the syringe action is faulty the syringe may need to be replaced see Section 5 11 e Ifthe syringe does not move in response to a command the cable may need to be replaced Contact Dionex Time out occurred waiting for syringe serial port txrdy Possible Causes Blocked syringe lines Faulty syringe Faulty cable Actions e Check the lines to the syringe for crimps or other blockages Replace the lines if necessary see Section 5 1 Check the syringe motion If the syringe action is faulty the syringe may need to be rep
63. the sample temperature control option is installed This field is for support of the thermal compartment option which is not currently available Specifies whether the 550 should wait until the temperature of the sample tray has stabilized before continuing Values 4 to 60 C default off default set point 20 YES NO Doc 031935 01 4 04 C Display Screens C 4 3 Timed Events Screen Use the TIMED EVENTS screen to specify a sequence of events after the method s sample prep phase is completed TIMED EVENTS METHOD 25 TIME VALVE CSV TTL1 TTL2 RLY1 RLY2 INIT LOAD A 0 0 0 0 0 00 INJ ECT Help Prompt Figure C 8 Timed Events Screen When entering timed event parameters Press Select or Select V to select a value Press Delete to clear a value from a field When a field is blank the value from the previous step remains in effect Screen Field Description Values TIME The elapsed time at which the method step starts 0 to 999 99 Every method begins with an INIT initial step and minutes a TIME 0 00 step You can edit the events for these steps but cannot delete them The initial step occurs when the timed events for the previous injection have ended VALVE Controls the position of the injection valve in the IC LOAD system module An AS50 TTL output must be INJECT connected to a TTL input on the IC system module See Appendix D for details about TTL connections Only one LOAD and on
64. the tray remains stationary and a sampling needle arm moves from vial to vial Up to 99 injections can be taken from each vial The AS50 can be controlled locally from the front panel keypad and display or remotely with a personal computer running Windows 2000 or Windows XP and Chromeleon amp Chromatography Data Management System software Release 6 6 or later Limited remote control is also available using TTL and relay signals For communication between the AS50 and Chromeleon the AS50 is connected to a USB Universal Serial Bus port on the computer a USB hub or on another Dionex module for example an ICS 2000 Doc 031935 01 4 04 1 1 AutoSelect AS50 A utosampler Chapter 1 Introduction Chapter 2 1 2 About This Manual Introduces the AS50 explains the conventions used in this manual including safety related information Describes the 550 operating features and system Features Overview components provides the theory of operation Chapter 3 Operation and Maintenance Chapter 4 Troubleshooting Chapter 5 Service Appendix A Specifications Appendix B Installation Chapter C Display Screens Appendix D TTL and Relay Control Appendix E Simultaneous Injection Appendix F Unpacking Instructions NOTE Provides operating instructions using the 550 front panel buttons and screens and describes routine preventive maintenance procedures Lists problems and presents step by step
65. to make sure no air remains 5 2 3 Connecting the New Syringe and Flushing 1 Position the syringe under the valve 2 Holding the syringe at the top and bottom screw it back into the valve and tighten fingertight 3 Align the syringe piston with the connecting screw mount on the syringe drive 4 Insertthe syringe drive connecting screw removed in Section 5 2 1 and tighten fingertight 5 Perform a flush cycle a Press Menu Menu and 6 to go to the FLUSH PRIME screen b Move the cursor to the ACTION field and press Select A or Select V to select FLUSH Press Enter 6 Check the syringe for bubbles If any exist remove the syringe and remove the bubbles see Section 5 2 2 Reconnect the syringe and flush again 5 2 4 Initializing the Syringe 1 From the MENU of SCREENS press 5 and 3 to go to the PLUMBING CONFIGURATION screen PLUMBING CONFIGURATION Bu C 25 SAMPLE SYRINGE VOLUME 250 uL SAMPLE SYRINGE CONFIG READY PREPS CRINGE VOLUME OE Minis PREP SYRINGE CONFIG READY Help Prompt Figure 5 2 Plumbing Configuration Screen Doc 031935 01 4 04 5 5 AutoSelect AS50 Autosampler 2 Move the cursor to the SAMPLE SYRINGE CONFIG field or the PREP SYRINGE CONFIG field depending on which syringe is to be removed Press Select A to select the INITIALIZE option 4 Press Enter 5 The syringe plunger moves down a small amount This is its initial positi
66. zu schweren Schaden am Gerat f hren kann jedoch VORSICHT keine Verletzungsgefahr besteht Notes Informational messages also appear throughout this manual These are labeled NOTE and are in bold type NOTE NOTES call attention to certain information They alert you to an unexpected result of an action suggest how to optimize instrument performance etc 1 4 Doc 031935 01 4 04 1 Introduction 1 2 2 Safety Labels The TUV GS C US Mark safety label and the CE Mark label on the ASS50 indicate that the AS50 is in compliance with the following standards EN 61010 1 2001 safety CAN CSA C22 2 No 1010 1 92 A2 97 safety UL 61010C 1 2002 R8 02 safety and EN 61326 1997 A1 1998 EMC susceptibility and immunity The symbols below appear on the AS50 or on AS50 labels Alternating current Protective conductor terminal earth ground Power supply is on Power supply is off Indicates a potential hazard Refer to the operator s manual for an explanation of the hazard and how to proceed Doc 031935 01 4 04 1 5 AutoSelect AS50 Autosampler 1 6 Doc 031935 01 4 04 2 Description 2 1 Overview of Features Figure 2 1 illustrates the main features of the AutoSelect AS50 Autosampler Syringe and Reservoir Organizer Autosampler Compartment Figure 2 1 AS50 Operating Features Doc 031935 01 4 04 2 1 AutoSelect AS50 Autosampler Front Panel The front panel contai
67. 1 4 04 5 15 AutoSelect AS50 Autosampler 5 Insert the injection valve waste line into the small round opening at the right front corner of the drip tray see Figure 5 12 6 Route the needle seal line out the autosampler right side slot Route the needle seal line to the injection valve in the IC system Insert the waste line from the IC system s injection valve here Figure 5 12 Routing the Injection Valve Waste Line to the Drip Tray Valve Installed in Another Module To connect the waste line when two valves are installed in 550 autosampler compartment for simultaneous injections 1 Insert the waste line from the bottom injection valve into the small round opening at the right front corner of the drip tray 2 Insert the waste line from the top injection valve into the larger drain line opening see Figure 5 13 Insert the waste line from the bottom injection valve here Insert the waste line from the top gt 2 N injection valve SA here Figure 5 13 Injection Valve Waste Line Connections for Dual Valves 5 16 Doc 031935 01 4 04 5 Service 5 6 Replacing the Needle Seal Assembly 1 Remove the drip tray see Section 5 4 2 Disconnect the needle seal line fitting from port S 5 on the injection valve Using a 5 16 in wrench turn the fitting counterclockwise to loosen it and then complete the removal with your fingers If the 550 is e
68. 2 Injections per vial Specifying in a schedule 3 36 C 18 Zero 3 34 Inserting lines in a method 2 4 Inserting steps in a schedule 2 4 3 35 Installation B 1 Injection valve connections B 8 Injection valve connections for simultaneous injection E 3 Module setup B 14 PC connections B 10 Power cord B 12 Priming B 18 Reagent reservoir connections B 7 Syringe cable connections B 6 Syringe liquid line connections B 4 TTL relay connections D 2 USB connections B 10 Installation checklist B 3 Installed Options screen 3 3 B 13 C 19 With simultaneous injection option E 1 Installing the drip tray 5 15 Doc 031935 01 4 04 Index 5 AutoSelect AS50 Autosampler Integrator 2 16 Invalid leak sensor reading 4 15 Inverted edge TTL input control C 30 D 4 Inverted pulse TTL input control C 30 D 4 Isopropyl alcohol 5 4 K Key sound Turning on and off C 20 Keyboard Test screen C 42 Keypad 2 3 Keypad buttons 2 4 See also Buttons L LCD 2 2 2 3 2 6 Leak detected in tray syringe area 4 14 Leak sensor Calibrating 5 27 Replacing 5 27 Leak Sensor Calibration and Status screen C 41 Leak sensor needs recalibration 4 15 Leaks 4 21 Drain line connection 4 22 Fittings 4 22 Inject port 4 22 Liquid line 4 22 Syringe 4 21 Syringe valve port 4 21 Limited sample injections 3 38 Line numbers 3 35 C 17 Liquid Control screen C 37 Liquid liquid extractions 2 10 Lithium battery 5 1 Loading
69. 3 To select a prep operation move the cursor one field to the right and press Select A or Select V to scroll through the list of operations As you select each type of operation the parameters for that operation are displayed to the right 4 Press Enter or a cursor arrow to select an operation 5 After selecting an operation enter its parameters Refer to the following sections for details about each sample prep operation NOTE To delete a sample prep step position the cursor in the step s leftmost column and press Delete twice 3 20 Doc 031935 01 4 04 3 Operation and Maintenance Using the Wait Operation The wait operation puts the method on hold until the AS50 receives a run command from the front panel Hold Run button or a TTL input signal The AS50 inserts a single wait operation at the end of every method s sample prep phase e You cannot delete the wait operation from the sample prep phase but you can change its position or disable it The wait operation s position in the sample prep phase determines whether samples are run overlapped or sequentially When the wait is the last sample prep step the default samples are overlapped see Section 2 6 2 When the wait is the first sample prep step samples are run sequentially see Section 2 6 3 Changing the Position of the Wait Operation in the Sample Prep Phase insert a step above the wait line position the cursor on the wait line and p
70. 3 5 Main Screen Local Control Doc 031935 01 4 04 3 7 AutoSelect AS50 Autosampler 3 5 Running a Schedule of Injections from the Front Panel This section describes how to run a schedule of injections from the AS50 front panel To run a schedule sequence of injections from the computer refer to the Chromeleon Help or user s guide Before you can run a schedule you must first create a method and schedule See Section 3 10 for details about creating methods and Section 3 11 for details about creating schedules Notes e The ASSO can run a schedule while you are entering or editing any method or schedule even the one that is currently running Changes made to the currently running method or schedule will be implemented on the next injection When saving changes to the currently running method or schedule or switching to a different schedule the clock continues running unaffected Only those parameter changes which affect the schedule after the current time will be implemented in the current run The 550 can store up to nine 1 through 9 schedules in memory Methods and schedules are retained in memory even after the AS50 power is turned off 3 8 Doc 031935 01 4 04 3 Operation and Maintenance 3 5 1 Selecting and Starting the Schedule 1 Goto the MAIN STATUS or DETAIL STATUS screen 2 Position the cursor in the control mode field and press Select A or Select V to toggle to SCH sch
71. 30 431 438 Format of method schedule database has changed Reinitializing to default values Possible Causes Dead battery on CPU board Malfunctioning power supply Actions Contact Dionex Format of calibration database has changed Reinitializing to default values Possible Causes Dead battery on CPU board Malfunctioning power supply Actions Contact Dionex Timed out waiting for home position to be found Possible Causes e Autosampler compartment door open at power up e DSP malfunction e Sampling arm malfunction Actions Close the autosampler compartment door and then press the Home button e If closing the door does not eliminate the problem try turning the power off and then on again If the problem persists contact Dionex 4 12 Doc 031935 01 4 04 4 Troubleshooting 439 Method Schedule database has been corrupted Reinitializing database Possible Causes Dead battery on CPU board Malfunctioning power supply Actions Contact Dionex 441 Sampler door opened Possible Causes e Autosampler compartment door accidentally opened Door not closing properly Door sensor malfunction Door magnet missing Actions The autosampler door must be closed to operate the AS50 Close the door and restart the schedule if necessary e Ifthe door appears closed but the message still occurs check for an obstruction that is preventing the door from closing
72. 30 C9 Diagnostic Menu 0 cece een eee eee C 31 C 9 Power Up Screen osre ccceceia cie eee eee eee C 31 C 9 2 Diagnostic Tests Screen C 32 9 3 XYZ Vest Screens aute d aede C 33 C 9 4 Temperature Statistics C 35 Doc 031935 01 4 04 vii AutoSelect AS50 Autosampler C 10 C 11 C 9 5 Liquid Control Screen C 37 9 6 Eogs M nus te due C 39 C 9 7 Leak Sensor Calibration and Status Screen C 41 C 9 8 Keyboard Test C 42 C 9 9 Code Versions 43 Print Menu arise reete temet ct e np beg eM C 44 Time Function Out Screen C 46 D TTL and Relay Control D 1 D 2 TTL and Relay Connections D 1 1 1 Connecting a TTL or Relay D 2 D 1 2 Selecting TTL Input Control D 3 Controlling TTL and Relay D 5 D 2 1 Example TTL Relay Connections D 6 E Simultaneous Injections E 1 E 2 E 3 E 4 Injection Valves Optional E 2 Connecting the Injection E 3 E 2 1 Connecting to Valves Installed in the IC System Modules E 3 E
73. 46 mm is closest to the top of the vial 3 26 Doc 031935 01 4 04 3 Operation and Maintenance Conirolling the Time Between Injections Cycle Time The CYCLE TIME parameter is an optional feature that can be used to control the time between injections See Section 2 6 5 for details 1 Onthe METHOD SETUP screen position the cursor in the CYCLE TIME field 2 Ifthe field displays press Select A or Select V to toggle the cycle time to on Press numeric buttons to enter the desired time between injections 4 Press Enter Conirolling the Needle s Position in the Vial The SAMPLE NEEDLE HEIGHT parameter positions the tip of the needle at the specified distance above the bottom of the vial This height is always used for sample injections It is also the default height for mixing pipetting and dispensing unless a NEEDLE operation is specified in the sample prep steps see Section 3 10 3 1 the METHOD SETUP screen position the cursor in the SAMPLE NEEDLE HEIGHT field 2 Press numeric buttons to enter the height setting 3 Press Enter Setting the Tray Temperature The TRAY TEMPERATURE parameter is available only when the sample temperature control option is installed Otherwise this field will display and will not be illuminated 1 Onthe METHOD SETUP screen position the cursor in the TRAY TEMPERATURE field 2 Ifthe field displays tray temperature control is currently tu
74. 5 When multiple injections are scheduled from a single vial the sample prep phase is performed once only before the first injection The method setup and timed events are performed for each injection Doc 031935 01 4 04 3 Operation and Maintenance Specifying Vial Positions Vial positions in prep operations can be specified by an absolute number or a relative position specify an absolute number press the numeric button s for the desired position The valid vial positions depend on which type of tray is installed Tray Type Valid Vial Numbers 1 5 mL aluminum 1 to 100 10 mL plastic 1 to 49 To enter a relative position press Select A or Select V to cycle through the choices SMP is the sample vial specified for the current injection in the schedule S 1 is one vial past the sample vial S42 is two vials past the sample vial and so on up to S 9 WST is the waste port Defining Sample Prep Steps 1 METHOD MENU move the cursor to the 1 field SAMPLE PREP and press Enter to display theSAMPLE PREP screen see Figure 3 13 SAMPLE PREP METHOD 25 Help Prompt Figure 3 13 Sample Prep Screen Default Doc 031935 01 4 04 3 19 AutoSelect AS50 Autosampler 2 Press Insert to add a new step above the wait step A DELAY step of 0 minutes is added by default see Figure 3 14 SAMPLE PREP METHOD 25 0 0 min Help Prompt Figure 3 14 Example Sample Prep Screen
75. ATUS screen to Display detailed information about the status of AS50 operating parameters This screen displays all the information shown on the MAIN STATUS screen plus additional parameters Set several parameters for Direct Control operation NOTE See Section C 2 for descriptions of the parameters common to the MAIN STATUS and the DETAIL STATUS screens DETAIL INJ ECTING VIAL 2 1 TIME 0 0 imilin INJ 1 3 MOIS uL METHOD 01 TRAY P10mL 25 20 Cf LOOP 25 0 uL CON PARTIAL LOCAL SCH 1 LINE 1 Help prompt Figure C 4 Detail Status Screen NOTE If the simultaneous injection option is installed the CS VLV field is replaced by a second INJ VLV field to allow you to set the position of both injection valves See Appendix E for details C 6 Doc 031935 01 4 04 C Display Screens Screen Field TRAY C COL C TRAY LOOP Injection Type TTL1 TTL2 RLY1 RLY2 INJ VLV Description If the sample temperature control option is not installed is displayed and the field is not backlighted If the sample temperature control option is installed this field allows you to set the tray temperature set point in C The actual measured temperature and a symbol indicating the temperature status are displayed to the right of this The temperature is stabilized The temperature is increasing The temperature is decreasing The temperature control option is off lt I
76. Au moins deux personnes peuvent soulever l AS50 qui p se plus de 18 kg 40 Ib Ne soulevez l AS50 que par chaque c t du fond de l armoire Son soul vement par porte du panneau avant GARDE endommagera les de porte Der AS50 wiegt ber 18 kg Daher sollte das Ger t nur von zwei oder mehr Personen angehoben werden Greifen Sie dazu an beiden Seiten unter das Gerat Heben Sie den Autosampler nicht an der Vordert r VORSICHT an da dadurch die T rangeln besch digt werden k nnten Doc 031935 01 4 04 B 1 AutoSelect AS50 Autosampler B 2 Unpacking 1 Remove the top of the 550 shipping box 2 Follow the instructions in Appendix F to unpack the module After unpacking save the shipping restraints To stabilize the sampling arm and prevent damage always reattach these shipping restraints before moving or shipping the AS50 3 If desired set the 550 on the bench sideways while installing it to allow easy access to the rear panel connections Recommended System Layout Place the AS50 on the workbench to the left of the other system module s This allows the shortest liquid line connections between the AS50 and the chromatography components injection valve pump column etc B 2 Doc 031935 01 4 04 B Installation B 3 Autosampler Installation B 3 1 Before You Begin Install the chromatography system components pump detector co
77. B C andD PRIME All 2000 uL Repeat the priming procedure for each reservoir Reagent Holding Loop Front Fitting Back Fitting To From Needle Sample Syringe p Front Fitting Valve Sample Transfer Line Sample Syringe Syringe Reagent C TM MM Optional Sampling Reagent D ReagentA Flush Reservoir Needle Optional Figure B 14 Sample Preparation Option Fluid Schematic Doc 031935 01 4 04 B 19 AutoSelect AS50 Autosampler Priming Procedure 1 On MAIN screen set AS50 to LOCAL mode and DIRECT CONTROL 2 Press Menu and 6 to go to the FLUSH screen see Figure B 15 3 Enter the desired PRIME VOLUME See the previous guidelines for the suggested volumes 4 Ifthe sample preparation option is installed a Move the cursor to the PRIME RESERVOIR field and select the reservoir b Move the cursor to the PRIME SYRINGE field and select the PREP syringe If the sample preparation option is not installed the PRIME RESERVOIR and PRIME SYRINGE fields default to FLUSH and SAMPLE respectively and cannot be edited 5 Move the cursor to the PRIME ACTION field and select PRIME 6 Press Enter to start the prime cycle FLUSH PRIME FLUSH PRIME VOLUME 400 uL VOLUME 2000 uL RESERVOIR RESA SYRINGE SAMPLE SYRINGE PREP ACTION READY ACTION PRIME Help Prompt Figure B 15 Initiating a Priming Cycle 7 Repeat the procedure as
78. C 16 inject Port Alignment Screen X ADDRESS The X axis position of the needle arm 1290 to 1390 default 1340 Y ADDRESS The Y axis position of the needle arm 1880 to 1950 default 1930 Z ADDRESS The Z axis position of the needle arm 180 to 280 default 230 C 26 Doc 031935 01 4 04 C Display Screens C 6 7 Door Interlock Bypass Screen Use the DOOR INTERLOCK BYPASS screen to temporarily disable the autosampler compartment door lock DOOR INTERLOCK BYPASS DOOR INTERLOCK BYPASS YES NOTE Afer setting bypass you havea 15 minute window to perform operations with the sample door open Help Prompt Figure C 17 Door Interlock Bypass Screen Be aware of unexpected sampling arm movement when operating with the door open CAUTION Soyez conscient de la possibilit de mouvements impr vus du bras d chantillon lorsqu il est utilis avec la porte ouverte MISE EN GARDE Achten Sie beim Betrieb mit gedffneter T r auf unerwartete Bewegungen des Probenarms VORSICHT Screen Field Description DOORINTERLOCK Press Select A or Select V to select YES bypass the door BYPASS lock or NO do not bypass the door lock and press Enter The default setting is NO After selecting YES and pressing Enter the door lock alarm is disabled for 15 minutes If you open the autosampler compartment door during this time the sampling arm continues its current operation if a schedule is running it continues also To extend the
79. CONTROL screen see Section C 9 5 Ifthe problem persists contact Dionex Received invalid command from DSP Possible Causes Communication between the CPU and the DSP was corrupted Actions Turn off the power and then turn on the power again If the message reappears contact Dionex Schedule error Specified schedule does not exist Possible Causes The schedule selected to run has not been saved Actions Create the new schedule or select an existing schedule Doc 031935 01 4 04 4 Troubleshooting 398 408 409 Battery backed up RAM failed data validation test Setting system parameters to defaults and initializing schedule and method database Possible Causes Temporary short on CPU board Dead battery on CPU board Malfunctioning power supply Actions If a temporary short is suspected the AS50 can still be operated You will need to re enter any method and schedule information and customized system setup parameters If the problem persists contact Dionex Recoverable motion error occurred in X axis Possible Causes An error occurred during X axis movement of the sampling arm The DSP was able to recover from the error and continue the motion Actions Occasional occurrences of this error can be ignored If the problem persists contact Dionex Non recoverable motion error occurred in X axis Clear any physical obstructions and then press any key to reset system Pos
80. DISPLAY PANEL BACKLIGHT KEY ACTUATION SOUND ENTRY ERROR SOUND HELP LANGUAGE Figure C 12 Front Panel Screen Description Selects the brightness of the display panel backlight The off option darkens the display completely When off press any button to turn the light on again Toggles the key sound on and off Toggles the error sound on and off Selects the language for help and error messages Values LOW MEDIUM HIGH OFF default HIGH ON OFF default OFF ON OFF default OFF ENGLISH JAPANESE C 20 Doc 031935 01 4 04 C Display Screens C 6 3 Plumbing Configuration Screen Use the PLUMBING CONFIGURATION screen to specify the plumbing options installed in the 550 SAMPLE SYRINGE VOLUME SAMPLE SYRINGE CONFIG PREP SYRINGE VOLUME PREP SYRINGE CONFIG PLUMBING CONFIGURATION PEEK INJ ECT VALVE 25 Help Prompt Screen Field INJECT VALVE LOOP SIZE SAMPLE SYRINGE VOLUME SAMPLE SYRINGE CONFIG Description The injection valve material The size of the loop s installed The volume of the sample syringe installed Press Select A or Select V to cycle through the choices If the AS50 is equipped with the simultaneous injection option 5000 and 10000 LL sizes are added See Appendix E for details The action to be performed by the sample syringe These actions are used to configure the syringe at initial setup or
81. EDULE 2 2 2 _ METHOD FRONT DIAGNOSTIC SETUP PANEL TESTS 2 PRINT METHOD 3 3 3 PENES PLUMBING XYZ TEST CONFIGURATION 4 4 I TIME DATE TEMP Available only STATISTICS with the sample temperature option 5 SYSTEM PARAMETERS CONTROL 6 INJ ECT PORT 6 1065 ALIGNMENT MENU 1 USAGE 7 LOG INTERLOCK 7 BYPASS LEAK CAL amp STATUS 2 MESSAGE LOG 8 KEYBOARD TEST 9 cope VERSIONS 3 4 Doc 031935 01 4 04 3 Operation and Maintenance NOTE If the simultaneous injection mode is enabled several screens change to allow control of two injection valves Refer to Appendix E for details To access AS50 screens Press the Menu button to display a menu of screens select a screen from the menu press the keypad number button corresponding to the screen s number on the menu or move the cursor to highlight the screen number and press Enter Example To go to the SYSTEM PARAMETERS screen from the MAIN STATUS screen 1 Press the Menu button to display the MENU of SCREENS 2 Press 5 to go to the MODULE SETUP MENU 3 Press 5 again to go to the SYSTEM PARAMETERS screen To return to the MAIN STATUS screen 1 Press Menu twice 2 Press Enter or 1 To enter or change a value in a screen field NOTE You can enter or change screen field values only when the AS50 is in Local mode see Section 3 4 1 Press a cursor arrow button to position the cursor in the field 2 Enter the desired value
82. I Pressing Select A or Select V toggles the tray temperature control on and off The default is off When on the default set point is 20 C This field is for support of the thermal compartment option which is not currently available The tray type in use The size of the loop in microliters Note If the AS50 is equipped with two injection valves two loop sizes are shown The type of injection FULL for full loop injections PARTIAL for partial loop PARTIAL LS for partial loop limited sample and SIM for simultaneous injection This field is updated when the initial conditions of the method s timed events are executed Provides TTL and relay control of other devices In Direct Control select 1 on or 0 off In Schedule control set the TTL and relay fields from the TIMED EVENTS screen for the method being run Appendix D describes TTL and relay control Displays and lets you set the position of the injection valve Values 4 to 60 C default 20 NONE 2 mL P10 mL 10 mL plastic FULL PARTIAL PARTIAL LS SIM 1 on 0 off L load I inject Doc 031935 01 4 04 C 7 AutoSelect AS50 Autosampler Screen Field CS VLV INJ VLV 1 INJ VLV 2 simultaneous injection mode only Description Displays and lets you set the position of the optional column switching valve Note The column switching valve option is not currently available for the AS50 USB If the AS50 is equi
83. J VOL uL non simultaneous injection mode INJ VOL uL simultaneous injection mode METHOD Description The first and last vial numbers for the schedule step The vials in this range are sampled according to the parameters specified in the step The starting vial does not have to be a smaller number than the ending vial To sample just one vial enter the same vial number for the start and end The number of injections performed on each vial If zero injections is specified only the sample prep portion of the method will be performed The volume of sample delivered for each injection The maximum injection volume depends on the loop size installed The volume entered determines whether the injection is a full loop partial loop or partial loop limited sample See Section 3 12 for details If the AS50 is equipped with two injection valves for simultaneous injections the injection volume is loaded into the sample syringe and then delivered equally to the two sample loops See Appendix E for details about the simultaneous injection option The method number to run on each vial in the range Values 1 to 100 1 5 mL tray 1 to 49 plastic 10 mL tray 0 to 99 default 1 1 0 to 99 9 uL 100 to 1000 uL default 25 1000 to 5000 uL with 5 mL syringe 1000 to 8000 uL with 10 mL syringe default 1000 uL 1 to 99 Doc 031935 01 4 04 C Display Screens C 6 Module Setup Menu M
84. NEX vo 20 0 u TRAY COL 20 METHOD 01 LOCAL SCH jf LINE 1 Help Prompt EK c lt gt ocr Coen n v Adjust screen contrast Figure 2 2 AS50 Front Panel Information is displayed on the LCD or screen To adjust the screen contrast use the knurled knob in the recess below the keypad see Figure 2 2 From the keypad you can Directly control AS50 operation Create and modify programmed series of operating steps called methods Create and modify programmed series of injections called schedules Refer to Section 2 2 2 for a summary of how to select screens and edit parameters Doc 031935 01 4 04 2 3 AutoSelect AS50 Autosampler 2 2 1 Keypad Button Functions Button Function The following buttons directly control AS50 operation Fo St S op P Hold Ru A M Hold Hold Ru Run a A n os ne Home Door oA Stops a schedule if the AS50 is in Local mode Schedule control A confirmation message appears press Stop again to abort the schedule or Hold Run to resume If the AS50 is in Direct Control pressing Stop stops the current syringe and or sampling arm movement Note Pressing Stop while the AS50 is in Direct Control Locked Remote mode Chromeleon control is not recommended Use the controls on the Chromeleon Control panel to stop a sequence At power up the AS50 is on
85. ODULE SETUP MENU INSTALLED OPTIONS 6 INJECT PORT ALIGN FRONT PANEL 7 INTERLOCK BYPASS PLUMBING CONFIGURATION TIME DATE SYSTEM PARAMETERS elp Prompt Figure C 10 Module Setup Menu Screen C 6 1 Installed Options Screen The INSTALLED OPTIONS screen lists the optional features installed in the AS50 The AS50 automatically detects which options are installed and displays a check mark in the appropriate fields This screen is displayed briefly at power up INSTALLED OPTIONS SAMPLE TRAY TEMPERATURE CONTROL COLUMN TEMPERATURE CONTROL SAMPLE PREPARATION Iv COLUMN SWITCHING VALVE INJ ECTION VALVE v PEEK Help Prompt Figure C 11 Installed Options Screen NOTES The COLUMN TEMPERATURE CONTROL and COLUMN SWITCHING VALVE options are not currently available for the AS50 USB Ifthe simultaneous injection option is installed two injection valves are shown on the screen The injection valve option is always enabled even if a valve is not installed in the autosampler compartment This allows all injection valve commands issued from Chromeleon to control the valve in the IC system ICS 1000 ICS 1500 ICS 2000 Doc 031935 01 4 04 C 19 AutoSelect AS50 Autosampler C 6 2 Front Panel Screen Use the FRONT PANEL screen to select display options FRONT PANEL DISPLAY PANEL BACKLIGHT HIGH KEY ACTUATION SOUND ENTRY ERROR SOUND HELP LANGUAGE ENGLISH Help Prompt Screen Field
86. ON D 2 Doc 031935 01 4 04 D TTL and Relay Control c Plug the connector into the 12 pin connector on 550 rear panel d Connect the wires from 550 connector plug to the TTL or relay connector pins on the other module s Additional connector plugs are provided with other Dionex modules NOTE Check the polarity of each connection Connect signal wires to signal pins and ground wires to ground pins D 1 2 Selecting TTL Input Control Types The AS50 TTL inputs respond to four types of signals to accommodate different controlling devices The default control type Normal Edge is compatible with the output signals provided by Dionex modules If the device connected to the AS50 does not send a normal edge signal select the appropriate control type Refer to the documentation provided with the controlling device and the information below to select the correct type Select a different control mode from the TIME FUNCTION IN screen see Figure D 3 TIME FUNCTION IN MODE UNUSED 1 START CONTINUE 2 TRAY TEMP ON OFF 3 COLUMN TEMP ON OFF 4 Help Prompt NORMAL EDGE Figure D 3 Time Function In Screen Selecting Signal Modes TTL Input Control Types Normal Edge In normal edge operation the negative falling Action Off or No Effect 5 V edge of a signal turns on the TUUS function For example for the EDGE o y sss START CONTINUE function the negative edge
87. PLE PREP METHOD 25 SMP to 5 1 MIX 250 uL 10 times FLUSH 250 uL WAIT Help Prompt Figure C 6 Sample Prep Screen Prep Operation Description DELAY Specifies for how long the AS50 waits 0 0 to 999 9 minutes before proceeding to the next step in the sample prep phase default 0 0 NEEDLE The height of the needle above the bottom of the vial from 0 to 46 mm default 2 mm The needle height is used for all pipet dispense and mix operations until a new height is specified NOTE For 0 3 mL and 1 5 mL vials use a maximum needle height of 2 mm This ensures the vent in the sampling needle is positioned correctly inside the vial PIPET Moves a specified volume of sample from one vial to another The sample syringe is always used for pipetting uL Enter the volume to pipet from 1 0 to 99 9 uL in 0 1 uL increments or 100 to 1000 uL in 1 uL increments default 20 pL Enter the source vial position the vial from which to pick up the specified volume default S 1 See below for how to enter vial positions tos X Enter the destination vial position the vial to receive the volume of sample default SMP C 10 Doc 031935 01 4 04 C Display Screens Prep Operation MIX FLUSH DISPENSE Description To enter vial positions Press numeric buttons to specify an absolute vial position from 1 to 100 depending on the number of vial positions available in the currently installed tray or Press
88. Select V to increase or decrease the current values Move the cursor to the DATE field 4 Press numeric buttons to enter the day and year directly or press Select or Select V to increase or decrease the current values Press Select or Select V to select the month 5 After selecting the last option press Enter before exiting the screen Doc 031935 01 4 04 B 15 AutoSelect AS50 Autosampler Enable or Disable the Wait Operation NOTE Do not complete this section if the AS50 is controlled by Chromeleon The software enables the wait function automatically and the setting on the SYSTEM PARAMETERS screen is ignored If the AS50 will be controlled by another instrument through TTL or relay connections set the WAIT field to ENABLED If the AS50 will control other modules system master through TTL or relay connections set the WAIT field to DISABLED 1 From the MODULE SETUP MENU press 5 to go the SYSTEM PARAMETERS screen SYSTEM PARAMETERS WAIT ENABLED SYRINGE CUT SEGMENT VOLUME 10 uL INJ ECT PORT VOLUME 43 uL SRS POWER SWITCHING DISABLED Help Prompt Figure B 12 System Parameters Screen 2 Position the cursor in the WAIT field and press Select A or Select V to select either ENABLED or DISABLED 3 Press Enter Notes When Wait is enabled the AS50 waits for a continue command either from the front panel Hold Run button or from a TTL input before performing the next lin
89. Sie das Netzkabel aus der Steckdose und der Netzbuchse auf der R ckseite des AS50 WARNUNG 2 Arecessed lock is located on each side of the fuse holder see Figure 5 26 Using a small screwdriver push each lock toward the center to release it The fuse holder pops out approximately 0 16 cm 1 16 in when the locks release When both locks are released pull the fuse holder straight out of its compartment Main Power Receptacle Fuses 2 pm Locking Spring Fa Key Insert screwdriver Fuse Holder and twist to release side view each side Fuse Holder Figure 5 26 Autosampler Compartment Main Power Fuse Holder 3 The holder contains two fuses Replace both with new 3 15 amp fast blow IEC 127 fuses P N 954745 Dionex recommends replacing both fuses Doc 031935 01 4 04 5 33 AutoSelect AS50 Autosampler 4 Reinsert the fuse holder into its compartment The fuse holder is keyed to fit only in its proper orientation Apply sufficient pressure evenly against the holder to engage the two locks The holder is flush against the panel when both locks are engaged 5 Reconnect the main power cord and turn on the power 5 15 Using the Chromeleon Wellness Panel 5 15 1 Opening the Wellness Panel 1 Open the Chromeleon Browser and expand the Dionex Templates folder 2 Expand the Panels folder and then the Wellness folder 3 On the right pane of the Browser window double click Dionex_autosampler_w
90. TL and Relay Control D 2 Controlling TTL and Relay Outputs The AS50 provides two TTL outputs and two relay contacts for control of functions in external devices such as an integrator The relay outputs can be used to switch any low voltage control Switched current must be less than 200 mA and 60 V peak blocking The relay contact closures are normally open When the relay Is closed current flows to the connected device Relay loads in excess of 200 mA or with included power supplies over 60 V may damage the relay drivers on the CPU CAUTION The TTL outputs are normally at 5 volts Setting a TTL output to 0 volts turns on the action in the connected device After connecting the TTL and Relay outputs see Section D 1 toggle the output states on and off in Direct Control from the DETAIL screen see Figure D 4 or in Schedule Control during the timed events portion of the method You can also use Chromeleon to control the outputs Toturn ona TTL or relay output set the corresponding output field on the DETAIL screen or method TIMED EVENTS screen to 1 on e To turn off a TTL or relay output set the corresponding output field to 0 off DETAIL DIRE VIAL min Wea INJ MOE uL METHOD TRAY 2 mL REY TRAY 25 20 el LOOP 100 uL RLY2 COLES INJ VLV LOCAL DIRECT CONTROL VIRA Help Prompt Figure D 4 Detail Status Screen Direct Control of TTL and Relays D 5 Doc 031935 01 4 04
91. TO 1 RUN 1 MISSING VIAL ACTION SKIP VIAL INJ INJ LINE START END VIAL VOL uL METHOD 1 4 1 4 3 25 0 5 5 5 1 25 0 6 6 6 1 5 0 Help Prompt Figure C 9 Schedule Screen Screen Field Description Values EDIT The schedule number to edit Press a numeric 1 to 9 button to select the number or press Select A or Select V to cycle through the choices Press Enter to display the selected schedule SAVE TO The schedule number to save the current schedule 1to9 to It does not have to be the EDIT number RUN The schedule number to run Note Entering a 1 to 9 value in this field does not start the schedule Press Hold Run or send a TTL RLY run command to start Missing Vial The action the AS50 takes if a vial is missing from SKIP STOP Action the tray Select SKIP to ignore the missing vial and default STOP move to the next vial in the schedule Select STOP to stop the schedule To resume press Hold Run LINE Each line contains the parameters for one vial in 1 to 999 the schedule Line numbers are assigned automatically automatically when you insert steps A step is assigned one horizontal line on the SCHEDULE screen If consecutive vials have identical parameter values line numbers are shown in a range format see the example above A schedule can have a maximum of 203 steps containing up to 999 lines Doc 031935 01 4 04 C 17 AutoSelect AS50 Autosampler Screen Field VIAL START END INJ VIAL IN
92. XYZHOME P INTERLOCK P INJ ECT VALVE Figure C 22 Diagnostic Tests Screen At power up these tests are run automatically If any tests fail the screen is displayed and indicates the status of each test The test was not run gt The test is in progress P The test passed F The test failed To run a test manually position the cursor in the edit field next to the test press Select A or Select V to select the asterisk and press Enter Test CPU LEAKS DSP COMM XYZ HOME SYRINGE COMM INTERLOCK INJECT VALVE INJECT VALVE 1 INJECT VALVE 2 simultaneous injection mode Description Checks the CPU internal configuration and the Moduleware checksum Checks whether the leak sensors detect a leak Checks communication between the CPU and the DSP Digital Signal Processor hardware Tells the DSP to search for the XYZ home position Checks the communication between the CPU and the sample syringe Checks that the autosampler door is closed Checks that the injection valve operates correctly If the AS50 is equipped with two injection valves for simultaneous injections these tests check that each valve operates correctly C 32 Doc 031935 01 4 04 C Display Screens C 9 3 XYZ Test Screen Use the XYZ TEST screen to test operation of the sampling needle arm 5 X i Z ACTION CYCLE ACTUAL 1234 5678 901 X SENSOR 0 fee 2340856788 901 Y SENSOR 2
93. adjusted allowing operations such as liquid liquid extraction 2 10 Doc 031935 01 4 04 2 Description 2 3 3 Flush Waste and Inject Ports The flush port flushes the outside of the sampling needle with fluid from the flush reservoir Excess fluid flows to the waste port The waste port accepts waste fluid from the sampling needle and the flush port and directs it out the drain line The inject port see Figure 2 6 accepts the sample from the sampling needle and delivers it to the injection valve Waste Port Flush Port Inject Port Figure 2 6 Flush Waste and Inject Ports 2 3 4 Sample Vials and Trays Vials The following vials are available 0 3 mL polymer P N 055428 package of 100 1 5 mL glass P N 055427 package of 100 10 mL PolyVial P N 055058 package of 100 Vial Trays The following tray types are available Tray Material Vial Size Held Tray Capacity Part Number Plastic 10 mL 49 055056 Aluminum 0 3 mL 1 5 mL 100 055057 Insulated Aluminum 0 3 mL 1 5 mL 100 061407 For use with the sample temperature control option only Doc 031935 01 4 04 2 11 AutoSelect AS50 Autosampler The AS50 automatically detects the type of tray installed Dionex does not recommend using the 10 mL plastic tray with the sample temperature control option because the plastic tray is inefficient at heating and cooling the vials In addition the tray is not designed for tempe
94. after a new syringe is installed INITIALIZE brings the syringe to an initial position SET HOME sets the topmost position the syringe will reach during operation READY is displayed when syringe configuration is complete no action occurs See Section 5 2 for details uL 250 uL READY 2 5 READY Figure C 13 Plumbing Configuration Screen Values PEEK 2 to 99 9 uL 100 to 1100 uL default 25 100 uL 250 uL 500 uL 1000 uL default 250 For simultaneous injection mode only 5000 uL 10 000 uL default 5000 SET HOME INITIALIZE READY Doc 031935 01 4 04 C 21 AutoSelect AS50 Autosampler Screen Field PREP SYRINGE VOLUME PREP SYRINGE CONFIG Description The volume of the prep syringe installed Press Select A or Select V to cycle through the choices The action to be performed by the prep syringe See the SAMPLE SYRINGE CONFIG section for details Values 2 5 mL 5 mL 10 mL default 5 SET HOME INITIALIZE READY C 22 Doc 031935 01 4 04 C Display Screens C 6 4 Time Date Screen Use the TIME DATE screen to set the time and date on the real time clock A 56 16 JUNE 04 Help Prompt Figure C 14 Time Date Screen ACTUAL TIME Displays the time on the real time clock SET TIME Sets the 24 hour real time clock Enter the hour first followed by minutes and then seconds For example if the time is 8 35 a m and 50 seconds enter 08 35 50 If t
95. ait for Cycle Time 1 The schedule either i stops and waits or skips to the next vial depending on Method Time 0 0 Inject the action selected in the schedule 2 If multiple injections are Method Times x x x x Set TTLs Relays etc specified from the same vial the sample prep steps are perfomed once only 4 before the first injection 3 Use of Cycle Time is Method Finished optional Figure 2 18 Order of Events During a Schedule Run Wait Disabled Doc 031935 01 4 04 2 29 AutoSelect AS50 Autosampler 2 6 7 Operating Events During Flushing and Priming The 550 automatically flushes the inject port and washes the outside of the needle at the beginning and end of a schedule as well as before each injection while the schedule is running Flushing can also be performed manually when the AS50 is idle or as a step in the sample prep phase of a method Priming of the flush and reagent lines is done manually after refilling a reservoir or after the AS50 has been idle for a period of time Flushing The AS50 performs the following operations during flushing e The sampling needle arm moves to the waste port and expels any fluid from the needle The needle arm then moves to the inject port and delivers a volume of fluid for flushing the inject port If the injection valve is set to the Load position the sample loop is also flus
96. ar panel see Figure B 3 10 Ifthe sample preparation option is installed connect the cable from the prep syringe to the PREP SYRINGE connector on the rear panel Sample Syringe Cable Prep Syringe Cable Optional Figure B 3 Rear Panel Connections B 6 Doc 031935 01 4 04 B Installation Sample Preparation Option Setup 1 Hill the reagent reservoir s P N 054808 with the desired reagent s and place them in the organizer 2 If you are installing a single reagent reservoir connect the line that extends from port A on the prep syringe valve to the reservoir cap 3 If you are installing additional reagent reservoirs connect the lines from ports B C and or D to the additional reservoirs NOTE Port letters A through D on the prep syringe valve correspond to reagent reservoirs A D on the AS50 front panel screens and in Chromeleon software Reagent Reservoir A Figure B 4 Sample Preparation Option Connections Doc 031935 01 4 04 B 7 AutoSelect AS50 Autosampler B 3 4 Connecting the Injection Valve NOTE If your AS50 is equipped with the simultaneous injection option refer to Appendix E for set up instructions 1 Open the 550 and IC system module doors 2 The needle seal line that extends from the inject port in the autosampler is coiled inside the AS50 door on the right side Route this line through the side slot on the AS50 see Figure B 5 and then thr
97. as System 2 on the Control panel Dionex ICS 2000 System Solvents Eluent Generator State Devices Error Levels Demo Chromatogram TTL Inputs Trend Calibration Diagnostics General Options Signals HeadTwee amp Limis Device Name Pump ECD 2 Mode Live Module Serial No e 1 0 0 Download C Virtual Use Live mode to control a physical module connected to the server Virtual mode can be used to demonstrate the software support for the module and allows one to create methods and panels without having a physical module attached to the server Doc 031935 01 4 04 E 11 AutoSelect AS50 Autosampler In addition to renaming the device name on the General tab page you will also need to rename signals and other devices associated with System 2 For example if System 2 is an ICS 2000 as in the previous example select the Signals tab click Change and add _2 to the signal name Repeat for the remaining signals See the following example NOTE You only need to rename the enabled signals i e the signals with checks next to their names Dionex ICS 2000 System x Solvents Eluent Generator State Devices Error Levels Demo Chromatogram TTL Inputs Trend Calibration Diagnostics General Options Signals Head Type amp Limits Factor us 1 00000 ECD Total 2 us 1 00000 Channel Pressure 2 psi 1 00000 Deactivate check boxes to
98. as Netzkabel ist das wichtigste Mittel zur Stromunterbrechung Stellen Sie sicher daB sich die Steckdose nahe am Ger t befindet und leicht zuganglich ist gt VORSICHT B 12 Doc 031935 01 4 04 B Installation B 3 7 Turning on the Power Press the power switch in the lower left corner of the front door to turn on the AS50 power When the power is turned on a series of screens are displayed and the autosampler performs a power up sequence The COPYRIGHT NOTICE screen is displayed briefly and then the POWER UP screen appears While the POWER UP screen is displayed the AS50 performs initialization procedures and a series of diagnostic tests If one of the tests fails testing stops and the DIAGNOSTIC TEST screen is displayed See Section C 9 2 if this occurs e When the initialization procedures are complete and the 550 has passed the diagnostics the INSTALLED OPTIONS screen appears After a few seconds or if you press a button the MAIN screen is displayed The power up sequence is then complete Doc 031935 01 4 04 AutoSelect AS50 Autosampler B 3 8 Module Setup Select Plumbing Configuration Options 1 2 Press Menu 5 to go to the MODULE SETUP MENU Press 3 to go to the PLUMBING CONFIGURATION screen PLUMBING CONFIGURATION INJ ECT VALVE PEEK 25 MU SAMPLE SYRINGE VOLUME 250 uL SAMPLE SYRINGE CONFIG READY PREP SYRINGE VOLUME 2 5 mL PREP SYRINGE CONFIG READY
99. as made to move a syringe valve to an illegal position 4 5 An attempt was made to move the syringe to an illegal position 4 5 An attempt was made to set an illegal syringe speed 4 6 An attempt was made to use the sample prep syringe 4 3 An illegal vial number was specified 4 7 Battery backed up RAM failed data validation test 4 9 Cannot run schedule No DSP detected 4 18 Cannot run schedule No sample syringe detected 4 19 Doc 031935 01 4 04 Index 3 AutoSelect AS50 Autosampler Cannot use sample syringe to aspirate from specified source reservoir 4 6 Current leak sensor voltage is outside of legal calibration range 4 15 Cycle time expired before INIT step was completed 4 10 DSP does not acknowledge 4 2 DSP not able to home XYZ motor arm 4 11 Format of calibration database has changed 4 12 Format of global database has changed Reinitializing to default values 4 11 Format of method schedule database has changed 4 12 Invalid leak sensor reading 4 15 Leak detected in tray syringe area 4 14 Leak sensor needs recalibration 4 15 Maximum number of steps reached 4 1 Memory is full Cannot save additional method or schedule 4 2 Method does not exist 4 2 Method Schedule database has been corrupted 4 13 Motion command to DSP was aborted 4 15 Non recoverable error Both internal and external inject valves are connected 4 19 Non recoverable motion error occurred in X axis 4 9 Ope
100. ations D 1 Tubing Replacing 5 2 U Unpacking B 2 Unpacking instructions F 1 Usage Log screen C 39 USB 1 1 Cable specifications B 11 Connecting to a hub B 10 B 11 Connecting to the PC B 10 Rear panel receptable 2 15 V Valve injection Connections for simultaneous injections E 3 E 5 Controlling 3 30 C 15 For simultaneous injections E 2 Specifications A 2 Valve prep syringe Removing 5 28 Replacing 5 31 Valve sample syringe Removing 5 28 Replacing 5 29 Vial trays 2 11 Vials Defining sampling parameters for 3 35 Filling 3 1 Loading in tray 3 2 Maximum fill level 3 1 Missing in a schedule 3 34 Specifying a range 3 36 Specifying positions in a method 3 19 Types of 2 11 A 2 Viscosity 3 45 C 24 Volume of prep syringe B 14 C 22 Volume of sample injected 3 33 3 41 Volume of sample syringe B 14 C 21 W Wait For Temp Stable option 2 26 3 28 C 14 Wait operation 3 18 3 21 C 12 Changing the position of 3 21 Cycle time and 2 26 Disabling 2 25 3 21 C 24 Enabling 3 21 C 24 Overview of 2 22 Waiting between sample prep steps Delay 3 18 C 10 Waiting for cycle time to expire 3 13 Washing the needle 2 30 Waste lines Connection to AS50 B 8 Leaking connection 4 22 Waste port 2 11 Weight A 1 Wellness panel 2 19 Windows 2000 1 1 Windows XP 1 1 X X address C 26 X and Y sensors C 33 XYZ Test screen C 33 Index 12 Doc 031935 01 4 04 Index
101. atus Line top of screen IDLE PREPARING TO RUN SCHEDULE HOLDING CHECKING FOR SAMPLE VIAL PRESENCE PIPETTING DISPENSING MIXING The top line on the screen displays the instrument s current operating status The following status values are displayed as they occur No method is currently running The AS50 is performing pre schedule operations such as flushing The schedule and method are on hold At the beginning of a method the AS50 checks that the specified sample vial is present The AS50 is pipetting from one vial to another during the method s sample prep steps The AS50 is dispensing from a reservoir during the method s sample prep steps The AS50 is mixing the vial contents during the method s sample prep steps Doc 031935 01 4 04 C 3 AutoSelect AS50 Autosampler Screen Field Description FLUSHING DELAYING xx x min DILUTE WAITING WAITING FOR RUN TO FINISH EXECUTING SETUP AND INIT STEP WAITING FOR CYCLE TIME TO EXPIRE WAITING FOR TEMP TO STABILIZE EXECUTING TIMED EVENTS LOADING LOOP INJECTING ABORTING PLEASE WAIT The needle is being flushed either during sample prep or between schedule lines The AS50 is pausing for the period of time specified in the sample prep phase of the method The AS50 is performing a dilution as part of the sample prep phase of the method The AS50 is waiting for a command to continue running a met
102. blies Tubing and Fittings Part Number 057301 061290 054271 061267 044777 043276 043275 042857 Description PFA tubing 1 55 mm 0 062 in ID 1 8 in flangeless ferrule fitting 1 4 28 flangeless nut Description Needle seal assembly 18 in Needle seal assembly 18 in for the simultaneous injection option Sampling needle assembly 2 mL Sampling needle assembly 2 mL for the simultaneous injection option PEEK tubing 0 75 mm 0 030 in ID Double cone ferrule fitting 10 32 double cone bolt Sample loop 25 uL Syringe and Reservoir Connections Used For Connecting the flush reservoir to the sample syringe valve Used For Connecting the AS50 inject port to the injection valve Connecting the sampling needle arm to the sampling valve Connecting the injection valve port 6 waste to the 550 drip tray Connecting ports 1 and 4 on the injection valves if installed Doc 031935 01 4 04 5 Service 5 2 Replacing the Sample or Prep Syringe The table below lists part numbers for the available syringe sizes Syringe Type Syringe Size Part Number Sample 100 uL 055064 250 uL 053916 500 uL 055065 1000 uL 055066 5mL 053915 10 mL 055068 Prep 2 5 mL 055067 5mL 053915 10 mL 055068 A 5 or 10 mL syringe is required for the simultaneous injection option Doc 031935 01 4 04 5 3 AutoSelect AS50 Autosampler 5 2 1 5 2 2 Removing The Existing Syringe 1
103. bypass for another 15 minutes press Enter again This restarts the 15 minute window When the 15 minutes is up the DOOR INTERLOCK BYPASS setting automatically reverts to NO When NO is selected opening the autosampler compartment door stops the sampling arm operation and aborts the currently running schedule Doc 031935 01 4 04 C 27 AutoSelect AS50 Autosampler Flush Prime Screen Use the FLUSH PRIME screen to Set the parameters and initiate a flush cycle that flushes the inject port and washes the outside of the needle Set the parameters and initiate a prime cycle that primes the lines to the reservoir s and the sampling needle FLUSH PRIME PRIME 250 VOLUME 2000 uL RESERVOIRS IEUSK RESERVOIR RES A SYRINGE SAMPLE SYRINGE PREP ACTION READY ACTION READY Help Prompt Figure C 18 Flush Prime Screen Screen Field Description Values FLUSH The volume of fluid used to flush the inject port The 100 to 9999 uL VOLUME inject port is flushed using the volume specified and default2250 then the exterior of the needle is washed using a factory set volume FLUSH The flush reservoir is always used for flushing FLUSH RESERVOIR FLUSH The sample syringe is always used for flushing SAMPLE SYRINGE FLUSH To start a flush cycle press Select A or Select V to READY FLUSH ACTION select FLUSH and press Enter The 550 must be in Direct Control mode before starting the flush cycle
104. c Sample prep 2 32 Sample syringe 2 31 Screen contrast 2 3 Screen lighting C 20 Screens Overview of 3 4 See also Display screens Select buttons 2 5 Selecting options 2 5 Self Regenerating Suppressor Switching the power in dual channel systems C 25 Sensors Calibrating the leak sensor 5 27 Replacing the leak sensor 5 27 Vial present C 33 X and Y C 33 Septa 3 2 Sequence 2 19 E 13 Server Configuration program Starting B 17 Service 5 1 Aligning the inject port 5 19 Aligning the sampling needle 5 19 Calibrating the inject port volume 5 23 Calibrating the leak sensor 5 27 Changing the main power fuses 5 33 Installing the drip tray 5 15 Removing the drip tray 5 14 Removing the sample or prep syringe valve 5 28 Replacing the leak sensor 5 27 Replacing the needle seal assembly 5 17 Replacing the prep syringe valve 5 31 Replacing the sample or prep syringe 5 3 Replacing the sample syringe valve 5 29 Replacing the sampling needle 5 8 Replacing tubing and fittings 5 2 Setting the syringe home position 5 7 Setting up a method 3 26 Ship Kit B 1 Shipping instructions F 1 Shutdown 3 48 Signals Renaming E 12 Simultaneous injection option 1 1 E 1 Components E 1 Connecting to injection valves 3 5 Direct control of valves E 8 Injection valves E 1 Loop size E 7 Overview of 2 13 E 1 Sample syringe volume 2 9 E 7 Schedule control of valves E 9 Setting up Chromeleon E 10 Speci
105. calibration standards e Performs liquid liquid extractions by adjusting the height of the needle in the vial The following sample prep syringe volumes are available Syringe Volume Part Number 2 5 mL 055067 5 mL standard 053915 10 mL 055068 The reagent reservoir connects to Port A on the sample prep valve Additional reservoirs can be connected to ports B C and D Commands from the front panel screens or from Chromeleon let you select the reservoirs Sampling Needle Arm The autosampler door must remain closed during operation If the door is opened during operation the sampling arm stops immediately If a schedule is running it is aborted To safely open the door during a schedule press the Door button See Section 3 8 for details During operation the sampling needle arm moves the sample needle along X Y and Z axes To sample from a vial the arm moves to the vial s position in the tray and lowers the needle into the vial The sampling needle pierces the top of the sample vial to allow fluid to be drawn or delivered When sampling is complete the arm raises the needle out of the vial The vial tray is stationary throughout the sampling process When running a schedule of injections vials can be sampled in any order During a flush operation the sampling needle arm delivers flush fluid to the flush port see Figure 2 6 The needle height the distance from the tip of the needle to the bottom of the vial can be
106. ccuracy when performing partial loop injections specify an injection volume equal to half the loop volume or less For example if the loop is 100 uL use an injection volume of 50 uL or less For the best accuracy when performing partial loop limited sample injections specify an injection volume no greater than the loop volume minus the air bubble volume 8 uL For example if the loop is 25 uL use an injection volume of 17 uL or less Note The sample is positioned between two air bubbles when it is loaded into the loop 3 38 Doc 031935 01 4 04 3 Operation and Maintenance During an injection the DETAIL STATUS screen displays the type of injection REMOTE Help Prompt Injection Type Figure 3 20 Detail Status Screen Partial Loop Injection Type Doc 031935 01 4 04 3 39 AutoSelect AS50 Autosampler 3 12 1 Full Loop Injections When performing a full loop injection the 550 draws four times the loop volume from the sample vial and delivers it to the injection valve The middle portion of the sample is positioned in the loop and injected see Figure 3 21 NOTE For very small loop sizes less than 17 uL 2 5 times the loop volume plus 25 uL is delivered to the valve Sample Syringe D inject Port Waste Waste DEA NM R Z a Pump Column Column Column INJECT INJECT INJECT LOAD 1 Draw air 2 Draw sample 3 Draw air again
107. cle Cycles MIX SMP 250 uL 10 times Vial to Mix Volume Cycles 1to 100 aluminum tray 1to 9999 uL 1to 99 1 to 49 plastic tray SMP S 1 519 Doc 031935 01 4 04 3 23 AutoSelect AS50 Autosampler Flushing the Needle The sample syringe is used to flush the needle with the specified volume of liquid If you specify a volume greater than the syringe volume multiple flushes are done FLUSH 250 uL Volume to Flush Through Needle f Flush Volume 1 to 999 uL Dispensing from a Reagent Reservoir DISPENSE is available only if the sample preparation option is installed During dispensing the prep syringe moves a volume of reagent from a reservoir to a vial DISPENSE 1000 uL RESC to 100 Volume to Dispense Source Reservoir Destination Vial Volume Reservoir Destination Vial 1 0 to 99 9 uL RES A B 1to 100 aluminum tray 100 to 1000 uL C D 1 to 49 plastic tray SMP S 1 5 9 WST 3 24 Doc 031935 01 4 04 3 Operation and Maintenance Diluting DILUTE is available only if the sample preparation option is installed The following steps occur during a dilution The sample syringe picks up a volume of concentrate from the concentrate vial and dispenses it to the destination vial prep syringe dispenses a volume of diluent to the destination vial The sample syringe mixes the destination vial five times using
108. cle time and delays injection until the INIT step is complete See Section 2 6 5 for details Possible Causes The selected cycle time was too short Actions Select a longer cycle time see Section 3 10 4 4 10 Doc 031935 01 4 04 4 Troubleshooting 418 e 421 429 DSP not able to home XYZ motor arm Possible Causes Physical blockage of the sampling arm DSP malfunction Actions 1 Turn off the power 2 Check for an obstruction in the autosampler compartment 3 Check for freedom of movement in all three axes of the sampling arm 4 Turn on the power again If the problem persists contact Dionex Timed events step took so long to execute that at least one subsequent step was missed Possible Causes Insufficient time allowed for loading the sample loop Actions On the method s TIMED EVENTS screen set the injection valve to the load position in the INIT step the default e If the valve is not set to load in the INIT step be sure to allow enough time between the LOAD and the INJECT parameters for the loop to be completely loaded The time needed depends on the sample syringe volume and the selected syringe speed see Section 3 13 Format of global database has changed Reinitializing to default values Possible Causes Dead battery on CPU board Malfunctioning power supply Actions Contact Dionex Doc 031935 01 4 04 AutoSelect AS50 Autosampler 4
109. cut segment volume is a portion of sample that is discarded from each end of the aspirated sample The middle portion of the sample is positioned in the loop and injected see Figure 3 23 NOTE If the 550 is set up for simultaneous injections only full loop injections can be performed Sample j Syringe Y i I 1 9 I Inject Port I 1 Waste Waste Waste Waste Saw Mw 1 if N is AN ar Pump Column Pump Column INJECT INJECT INJECT 3 Draw air again to cushion the sample 1 Draw air 2 Draw sample LOAD 4 Deliver sample to the loop KEY Deionized Water wm Air mm Eluent wm Sample Figure 3 23 Partial Loop Injection Sequence 3 42 Doc 031935 01 4 04 3 Operation and Maintenance Setting Up a Partial Loop Injection 1 When defining injection parameters on the SCHEDULE screen enter a sample injection volume that is less than the volume of the sample loop installed on the injection valve On the SYSTEM PARAMETERS screen see Section C 6 5 enter a CUT SEGMENT VOLUME greater than zero The cut volume amount will be subtracted from both ends of the injected sample volume Example Sample loop volume 100 uL Injection volume 50 uL Cut segment volume 20 uL To perform the injection the AS50 aspirates 90 uL from the sample vial the inject volume plus double the cut volume and delivers it to
110. d when the valve was removed the slot in the drive shaft should be in the 11 o clock position see Figure 5 24 Prep Syringe Drive Shaft Initial Position Figure 5 24 Prep Syringe Drive Shaft Initial Position If the slot in the drive shaft is not in this position the syringe drive may need replacing Contact Dionex for assistance Do not attempt to manually turn the drive shaft 4 Checkthe back of the new prep syringe valve P N 054243 The valve drive shaft should be perpendicular to valve ports N and D see Figure 5 25 Back View of Valve Drive Shaft Initial Position Port D 4 On bottom of valve Not visible in drawing a Port N On top of valve Not visible in drawing Figure 5 25 Prep Syringe Valve Drive Shaft Initial Position If it is not rotate the valve rotor until the drive shaft is in the correct position To check the position insert the end of a paper clip into valve port N The paper clip should be inserted fully into the port Doc 031935 01 4 04 5 31 AutoSelect AS50 Autosampler 5 10 11 Each valve port must contain a white Teflon washer Check the inside of each port and insert a washer P N 061315 if one is not already present The washers are supplied with the syringe valve and with the AS50 Preventive Maintenance Kit Holding the syringe at the top fitting screw it into the bottom of th
111. e INJECT command can be specified in a method Sample loading must occur before the injection By default LOAD occurs at the INIT step and INJECT occurs at TIME 0 00 NOTE _ Ifthe schedule specifies an injection per vial of 0 then only the method s sample prep phase of steps occurs Doc 031935 01 4 04 C 15 AutoSelect AS50 Autosampler Screen Field VALVE1 VALVE2 simultaneous injection mode only CSV TTL1 TTL2 RLY1 RLY2 Description If the AS50 is equipped with or connected to two injection valves for simultaneous injections these fields are used to select the position of each valve Injection valve 1 is the bottom valve and injection valve 2 is the top valve Specify one LOAD and one INJECT command for each valve Sample loading must occur before the injection By default LOAD occurs at the INIT step and INJECT occurs at TIME 0 00 See Appendix E for details about the simultaneous injection option The position of the optional column switching valve Note The column switching valve option is not currently available for the AS50 USB Use the default setting A for this field Provides TTL and relay control of other devices See Appendix D for details Values LOAD INJECT A column A default B column B 1 on 0 off Doc 031935 01 4 04 C Display Screens C 5 Schedule Screen Use the SCHEDULE screen to program a sequence of injections SCHEDULE EDIT 1 SAVE
112. e WAIT field to DISABLED see Figure 2 15 SYSTEM PARAMETERS WAIT DISABLED SYRINGES REEDS CUT SEGMENT VOLUME O uL IN ECT RORY OLUIME 2 5 SRS POWER SWITCHING DISABLED Help Prompt Figure 2 15 System Parameters Screen For details about the order of events during a schedule when the wait is disabled see Figure 2 18 Doc 031935 01 4 04 2 25 AutoSelect AS50 Autosampler 2 6 5 Understanding the Cycle Time Cycle time is an optional feature that can be used to control the time between injections When a method is assigned a cycle time the AS50 delays sample injection until the specified time has elapsed since the previous injection Cycle time is measured from the start of the previous method s timed events see Figure 2 16 Assuming injection occurs at time zero for each method the default specifying a cycle time allows a consistent time interval between injections regardless of differences in sample prep and timed events among methods qvin m mee Cycle Time i Cycle Time Start First Method injection 1 WASH f ust auma nep ero TIMED EVENTS 4 Start Next Method Injection 2 i WASH mM nep nr vo EEN Next Method i Wait for end of cycle time WASH sanpLe nep sero ee eae FLUSH 00 Figure 2 16 Cycle Time Example A cycle s duration is never shorter than the specified time However cycle time will be ignored thus delaying
113. e a problem contact Dionex for help In the U S call Dionex Technical Support at 1 800 346 6390 Outside the U S call the nearest Dionex office Error Messages When an error occurs a beep sounds and a message appears Each message is identified by a number in brackets in the lower right corner of the AS50 screen To clear the message press any key Most error messages occur because you have pressed an incorrect key or attempted to enter an incorrect value for a particular field These types of messages generally do not need additional explanation and they are not listed here 232 Maximum number of steps reached A method can have a maximum of 100 steps including the sample prep steps the timed events steps and one step for the method setup parameters Possible Causes The method includes 100 steps Actions No action is required at this time However before trying to create a new step in the method delete an existing step Doc 031935 01 4 04 4 1 AutoSelect AS50 Autosampler 237 250 331 Memory is full Cannot save additional method or schedule The current method or schedule occupies all available memory Possible Causes Too many complex methods or schedules have been saved Actions To free memory for future use delete a method or schedule that is no longer used DSP does not acknowledge The DSP digital signal processor did not respond to a command from the CPU Possible Causes
114. e autosampler door Please wait Check the AS50 status above NOTE any key press will exit this mode Figure 3 9 Open Door Safety Screen Not Safe to Open Door This message remains until the AS50 reaches the correct status and the sampling arm is in the home position The screen then displays the message that it is safe to open the door see Figure 3 10 STATUS WAITING OPEN DOOR SAFETY SCREEN ATTENTION It is now safe to open the autosampler door Please note time left to safely open door Time 1 47 NOTE any key press will exit this mode Figure 3 10 Open Door Safety Screen Safe to Open Door The TIME field counts down the amount of time remaining in which it is safe to open the door When the door is opened the schedule goes to hold The timer continues counting down until the door is closed The schedule then resumes 3 12 Doc 031935 01 4 04 3 Operation and Maintenance If the door is still open when the timer reaches zero the schedule continues holding for up to 2 hours until the door is closed If the door is still open after 2 hours the schedule is aborted Notes e Ifthe 550 is operating under front panel control the door can be opened only when the sampling arm is at the home position and the status displays WAITING FOR CYCLE TIME TO EXPIRE The cycle time controls the time between injections see Section 2 6 5 and Section 3 10 4 For most runs selecting a cycle time greater than 4 minu
115. e in a schedule of injections See Section 2 6 1 for details When Wait is disabled a schedule of injections is started by pressing the Hold Run button The 550 then performs all injections in the schedule without waiting for any continue commands See Section 2 6 4 for details B 16 Doc 031935 01 4 04 B Installation B 3 9 Configuring the AS50 in Chromeleon If the 550 is connected to a Chromeleon PC follow the guidelines below to add the 550 to a Chromeleon timebase and connect it to a Control panel Refer to Installing the Chromeleon Chromatography Management System with a Dionex Ion Chromatograph IC Document No 031883 for detailed instructions Turn on the computer power and log onto Windows 2000 or Windows XP as an administrator If the Chromeleon Server is not running the Server Monitor icon on the taskbar is crossed out in red ak start the Server by right clicking the Server Monitor icon and selecting Start Server Start Chromeleon When the Unassigned Dionex USB Devices Detected dialog box appears add the AS50 to an existing timebase or create a new timebase In the Chromeleon Browser open a template Control panel go to the Dionex Templates gt Panels gt Dionex IC folder for the timebase Connect the AS50 timebase to the panel and then save the panel to a different folder Doc 031935 01 4 04 AutoSelect AS50 Autosampler B 3 10 Priming the Liquid Lines e See below for prim
116. e of vials in a schedule 3 36 Reagent reservoirs 2 9 Connecting B 7 Dispensing from 3 24 Real time clock C 23 Rear panel Features 2 14 RS 232 connector 2 14 TTL relay connectors 2 15 USB receptacle 2 15 Received invalid command from DSP 4 8 Recoverable motion error occurred 4 9 Reference Library CD ROM B 1 Relay connectors 2 15 Relay outputs Controlling 3 31 D 5 Voltage and current specifications D 2 Remote mode 2 19 Selecting 2 16 C 5 Removing Bubbles from the syringe 5 4 B 21 Drip tray 5 14 Sample or prep syringe valve 5 28 Syringes 5 4 Renaming devices E 11 Renaming signals E 12 Index 8 Doc 031935 01 4 04 Index Replacing Leak sensor 5 27 Needle seal assembly 5 17 Prep syringe valve 5 31 Sample or prep syringe 5 3 Sample syringe valve 5 29 Sampling needle 5 8 Tubing and fittings 5 2 Repsonse from syringe was too long 4 5 Reservoir organizer 2 2 2 9 Resuming a schedule 2 4 Retention time E 15 RS 232 interface Communication parameters C 45 Printer connection 2 14 Running a schedule of injections 3 8 C 17 S Safety messages 1 3 Sample delivery 1 1 2 9 Sample injection See Injections Sample loop E 2 For simultaneous injection 5 2 Loading E 2 See also Loop size and Loop volume Sample needle height See Needle height Sample prep Defining method steps 3 18 3 19 Delay operation 3 22 Dilute operation 3 25 Dispense operation 3 24 Flush operation 3 24 M
117. e valve at port S and tighten fingertight With valve ports L and B at the 12 o clock position and the syringe at the 6 o clock position push the valve onto the syringe drive shaft Replace the two valve thumbscrews and partially tighten Carefully align the syringe piston with the connecting screw mount on the syringe drive Insert the syringe drive connecting screw and tighten fingertight Finish tightening the valve thumbscrews Reconnect the valve liquid lines Tighten the fittings fingertight IMPORTANT If a fitting leaks when operation is resumed tighten it slightly Do not 12 13 overtighten Overtightening can damage the inside of the valve From the MENU of SCREENS press 5 and 3 to go to the PLUMBING CONFIGURATION screen Set the syringe home position see Section 5 2 5 On the PLUMBING CONFIGURATION screen move the cursor to the PREP SYRINGE VOLUME field Press Select A or Select V to select the syringe volume Press Enter 5 32 Doc 031935 01 4 04 5 Service 5 14 Changing the Main Power Fuses The fuse holder is part of the main power receptacle on the rear panel of the AS50 autosampler compartment 1 Turn off the main power HIGH VOLTAGE Disconnect the main power cord from its source and also from the rear panel of the AS50 WARNING HAUTE TENSION D branchez le cordon d alimentation principal de sa source et du panneau arriere du AS50 AVERTISSEMENT HOCHSPANNUNG Ziehen
118. eagents 2 9 3 18 3 24 C 11 Display screen backlight C 20 Display screens 2 6 C 1 Backlight C 2 Code Versions C 43 Detail Status 2 17 C 6 Diagnostic Menu C 31 Diagnostic Tests C 32 Flush Prime C 28 Front Panel C 20 Inject Port Alignment C 26 Keyboard Test C 42 Leak Sensor Calibration and Status C 41 Liquid Control C 37 Logs Menu C 39 Main Status 2 6 C 3 Menu of Screens 2 7 C 2 Message Log C 40 Method Menu C 9 Method Setup C 13 Module Setup Menu C 19 Plumbing Configuration C 21 Power Up 3 3 B 13 C 31 Print Menu C 44 Sample Prep C 10 Schedule 2 18 C 17 System Parameters 2 25 C 24 Temperature Statistics C 35 Time Function In C 30 C 46 Time Date C 23 Timed Events C 15 Usage Log C 39 XYZ Test C 33 Door button 2 4 Door opening during a schedule 2 4 3 12 Drain line B 4 Drain line leaks 4 22 Drip tray Installing 5 15 Removing 5 14 DSP does not acknowledge 4 2 DSP not able to home XYZ motor arm 4 11 Dual analyses E 1 See also Simultaneous injection option Duplicate column command E 14 E Editing a method 3 31 Editing a schedule 3 8 3 37 Editing screen fields 2 7 Electrical specifications A 1 Enabling the Wait operation 3 21 Enter button 2 5 Entering values in screen fields 3 5 Error message log C 40 Error messages 4 1 A failure has occurred during power up 4 3 A command was received from the DSP 4 7 An attempt w
119. ection valve to either LOAD or INJECT A method s timed events can include only one Load and one Inject command The Load command must precede the Inject By default the valve is set to LOAD in the INIT step and to INJECT in the time zero step This allows the valve to be loaded before timed events begin Then you do not need to include the time for loading the loop in the timed events You can clear the LOAD and INJECT parameters from the INIT and or time zero steps and then move them to other steps if desired To clear a LOAD or INJECT parameter position the cursor in the field and press Delete To move the LOAD or INJECT parameter to a different step position the cursor in the VALVE field of the desired step and press Select A or Select V to toggle to LOAD or INJECT Notes e Ifyou move the LOAD and or INJECT parameters to different steps be sure to allow enough time between the LOAD and the INJECT for the loop to be completely loaded The time needed depends on the sample syringe volume and the syringe speed see Section 3 13 Ifthe simultaneous injection option is installed you can set the positions of both valves in the timed events see Section E 4 3 30 Doc 031935 01 4 04 3 Operation and Maintenance 3 10 6 3 10 7 Setting TTL and Relay Outputs The AS50 has two TTL and two relay outputs for control of functions in other devices For example if you connect TTL output 1 on the AS50 to TTL input
120. ections from the Front Panel 3 8 Doc 031935 01 4 04 Contents 3 5 1 Selecting and Starting the Schedule 3 9 3 6 Putting a Schedule on 1 3 10 3 7 Stopping a Running Schedule 3 11 3 8 Opening the Autosampler Door During a Schedule 3 12 3 9 Running Under Direct Control from the Front Panel 3 14 3 10 Creating Methods from AS50 Front Panel 3 16 3 10 1 Creating a Method 3 17 3 10 2 Saving a 3 17 3 10 3 Defining Sample Prep 3 18 3 10 4 Selecting Method Setup Parameters 3 26 3 10 5 Defining Timed Events 3 28 3 10 6 Editing a Method 3 31 3 10 7 Deleting a Method 3 31 3 10 8 Copying a Method 3 32 3 10 9 Example Method 3 32 3 11 Creating Schedules from 550 Front Panel 3 33 3 11 1 Creating a New Schedule 3 33 3 11 2 Saving aSchedule eee cee ee eee 3 34 3 11 3 Selecting an Action if a Scheduled Vial Is Missing 3 34 3 11 4 Defining Schedule Lines 3 35 3 11 5 Editing a Schedule
121. edule control see Figure 3 6 LOCAL Help Prompt Control Mode Field Figure 3 6 Main Status Screen Schedule Control Mode Press Enter 4 Press a numeric button to select the desired schedule number or press Select A or Select V to cycle through the numbers 1 through 9 Press Enter 6 To start the schedule press the Hold Run button NOTE You can also select the schedule from the SCHEDULE screen see Figure 3 7 Enter the number in the RUN field and press Enter Press the Hold Run button to start the schedule Help Prompt Figure 3 7 Schedule Screen Doc 031935 01 4 04 3 9 AutoSelect AS50 Autosampler 3 6 Putting a Schedule on Hold To put a running schedule on hold press Hold Run or Stop The AS50 will finish the current method step before suspending operation While the schedule is on hold the time since the last injection continues to count up If the schedule is put on hold during a method s timed events the timed events clock is suspended until the schedule resumes See Section 2 6 8 for details about the clock displays To resume running the schedule press Hold Run NOTE If wait is enabled on the SYSTEM PARAMETERS screen see Section C 6 5 the schedule is automatically put on hold when it reaches the wait step in a sample prep phase The schedule will resume running only if a TTL signal is received see Section D 2 or if the Hold Run button is pressed 3 10 Doc 031935 01 4 04
122. efault sample needle height specified on the METHOD SETUP screen see Section 3 10 4 3 22 Doc 031935 01 4 04 3 Operation and Maintenance Pipetting Liquid from One Vial to Another The PIPET operation uses the sample syringe to transfer liquid from a source vial to a destination vial After pipetting mix the destination vial before sampling from it Be sure to specify enough mix cycles to completely mix the vial contents see Mixing a Vial below Volume to Pipet Source Vial Destination Vial PIPET 20 0 uL 5 1 to SMP Volume to Pipet Source Vial Destination Vial 1 0 to 99 9 uL 1 to 100 aluminum tray 1to 100 100 to 1000 uL 1 0 49 plastic tray 1 to 49 SMP S 1 5 9 SMP S41 S49 WST NOTE Unlike the DILUTE operation the PIPET operation does not include an automatic mixing step If the sample preparation option is installed use the DILUTE operation not PIPET for dilutions see Diluting on page 3 25 Mixing a Vial The 550 mixes the vial by repeatedly drawing up and expelling a volume of the contents The prep syringe is used if the sample preparation option is installed otherwise the sample syringe is used When specifying the volume enter a value that is less than or equal to the volume of the syringe If the volume is greater than the syringe volume an error message occurs and the syringe volume is used Volume to Aspirate and Number of Vial to Expel per Cy
123. ellness pan The Wellness panel appears 4 Open the Control menu and click Connect to Timebase Connect to the timebase in which the AS50 is configured When connected the controls on the Wellness panel are enabled see Figure 5 27 Autosampler Timebase System_A Computer name AMAR Calibration Diagnostic Calibration Details leak detector tray 245V 2 18 2004 external inject port volume 20 pl 2 18 2004 Diagnostic Tests leak detector tray 33 2 18 2004 external Figure 5 27 Chromeleon Wellness Panel 5 34 Doc 031935 01 4 04 5 Service 5 6 7 To calibrate the leak detector click the tray button under Calibration The AS50 calibrates the sensor and then uploads the new value to Chromeleon Chromeleon stores the new calibration value as the current value To test the leak sensor click tray under Diagnostic Tests The AS50 tests the sensor and then uploads the results to Chromeleon To enter a new inject port volume after calibration see Section 5 8 type the new volume into the volume field under Calibration inject port and press Enter Chromeleon downloads the value to the autosampler and stores this calibration value as the current value Doc 031935 01 4 04 5 35 AutoSelect AS50 Autosampler 5 36 Doc 031935 01 4
124. ents During a Schedule Run Wait Enabled 2 28 Doc 031935 01 4 04 2 Description Schedule Started 4 Preparing to Run Flush Inject Port and Wash Needle 4 Method Starts for Injection 1 4 Flush Inject Port and Wash Needle I Yes i See Note 1 Prosoni Temperature s Wait for Temperature to Stabilize No Method Starts for Injection 2 Method Starts for Injection 3 i i Flush Inject Port and Wash Needle Continue as Shown for Method 2 Until Last Scheduled V See Note 1 am No Vial Present gt p Sample Prep Overlap See Note 2 Do Sample Prep Pipetting Mixing etc 4 Set Up Method 4 Execute INIT Step Load Loop Method Time 0 0 Inject Start Cycle Timing Timed Events Yes Method Times x x x x Set TTLs Relays etc i Method Finished See Note 3 Yes See Note 2 Injection Completed i Flush Inject Port and Wash Needle Schedule Ends Sample Prep Overlap Do Sample Prep Pipetting Mixing etc i Wait for Previous Method to Finish i Setup Method 4 Execute INIT Step Load Loop i Notes W
125. enu displays the MENU of SCREENS which provides top level access to the menu structure MENU of SCREENS MAIN STATUS 6 FLUSH PRIME DETAIL STATUS 7 TIME FUNCTION IN METHOD MENU 8 DIAGNOSTIC MENU SCHE DUIME 9 PRINT MENU MODULE SETUP MENU 10 TIME FUNCTION OUT Help Prompt Figure C 2 Menu of Screens There are two ways to select a screen from a menu Move the cursor to the field containing the screen number and press Enter Press the numeric button on the front panel keypad that corresponds to the screen number on the menu To display a brief description of each screen press Help NOTE If no keypad buttons are pressed within a 2 hour period the backlight automatically turns off To turn the backlight on again press any button on the keypad Press the button a second time if you want to activate its function NOTE If the simultaneous injection mode is enabled several screens change to allow control of two injection valves Refer to Appendix E for illustrations of these screens and for details about simultaneous injections C 2 Doc 031935 01 4 04 C Display Screens C 2 Main Status Screen Use the MAIN STATUS screen to Display the status of basic operating parameters Select operating and control modes VIAL 1 INJ TRAY 20 C COL LOCAL Help Prompt 9C IDSE TIME 99 9 min VOL 20 0uL METHOD 01 MSINE Figure C 3 Main Screen Screen Field Description St
126. f the problem persists contact Dionex 4 16 Doc 031935 01 4 04 4 Troubleshooting 452 453 454 Non recoverable motion error occurred in Z axis Clear any physical obstructions and then press any key to reset system Possible Causes e Vial obstructing motion Bent sampling needle Misaligned sampling needle arm Incorrect X Y position not over a vial Actions Remove the vial obstruction Ifthe needle is bent replace the sampling needle assembly see Section 5 3 Align the sampling needle arm in the inject port see Section 5 7 Turn off the power and check for freedom of movement of all three axes of the sampling arm Contact Dionex Recoverable motion error occurred in inject valve Possible Causes e Valve in wrong position Actions Turn off the power and then turn on the power again If the message reappears contact Dionex Non recoverable motion error occurred in inject valve Clear any physical obstructions and then press any key to reset system Possible Causes Valve in wrong position Actions Turn off the power and then turn on the power again If the message reappears contact Dionex Doc 031935 01 4 04 AutoSelect AS50 Autosampler 455 Recoverable motion error occurred in auxiliary valve Possible Causes Valve in wrong position Actions Turn off the power and then turn on the power again If the message reappears contact Dio
127. fying injection volume C 18 Specifying plumbing configuration E 7 Valve flow schematic E 2 Valve options for 2 2 Site requirements B 1 Skipping a missing vial 3 34 C 17 SMP 3 19 Software See Chromeleon Specifications A 1 Facility requirements B 1 Specified volume is larger than size of syringe 4 10 Specifying vial positions 3 19 Speed of syringe 3 45 Index 10 Doc 031935 01 4 04 Index Starting a schedule 3 9 Status during operation 2 6 2 33 Steps in a schedule 3 35 Stop button 2 4 Stopping Schedules 2 4 3 11 When a scheduled vial is missing 3 34 C 17 Syringe leaks 4 21 Syringe reports error 4 18 Syringe reports fatal error 4 18 Syringe speed 3 45 C 24 Syringes 2 2 2 9 Connecting the liquid lines B 4 Filling 5 4 Flow rates delivered by 3 45 Initializing 5 5 Manually controlling C 37 Prep syringe description of 2 9 Removing 5 4 Removing bubbles 5 4 B 21 Removing the valve 5 28 Replacing 5 3 Replacing the prep syringe valve 5 31 Replacing the sample syringe valve 5 29 Sample syringe description of 2 9 Setting the home position 5 7 Specifying the volume of B 14 System layouts B 2 System master 2 25 Setup for B 16 System Parameters screen 2 25 B 16 C 24 System shutdown 3 48 T Technical Support 5 1 Temperature Operating specifications A 1 Temperature control Sample 2 2 2 12 Setting the tray temperature 3 27 Temperature stabilization 3 28
128. h all components for each detector channel included in one component list Use the Duplicate Column command on the Peak Table Amount Table and Peak Tracking pages of the method to create two extra Ret Time columns associate each column with a different channel NOTE To add a column right click in the Ret Time column and select the Columns gt Duplicate Column command on the context menu See the Chromeleon Help topic How to Actions in the QNT Editor Defining the QNT Method for Several Detectors for detailed instructions In the following example Peak Table the Peak Name column includes the components from two detector channels one for cations and one for anions The Ret Time ECD 1 column is associated with the cation detector channel and displays only the cations The Ret Time ECD 2 column is associated with the anion detector channel and displays only the anions AMAR local System A Shared CC80 x Peak Hame Ret Time Standard Int Type Cal Type Peak 4 Lin Auto 3 267 min 0 250 AG External 3 700 min 0 100 AG External QOff Auto 3 900 min 0 250 AG External Lin Auto 4 400 min 0 250 AG External QOff Auto 5 087 min 0 250 AG External GOff Auto 5 550 min 0 250 AG External Lin Auto 5 770 min 0 250 External GOff Auto 6 500 min 0 100 External GOff Auto 7 300 min 0 150 AG External QOff Auto 8 100 min 0 200 AG External Qott Auto Magnesium Calcium Chlorate Nitrate Phosphate Sulfate
129. he method to run on each injection The action to take when a scheduled vial is missing Doc 031935 01 4 04 2 17 AutoSelect AS50 Autosampler You program save and edit schedules from the SCHEDULE screen see Figure 2 10 The 550 can store up to nine schedules in memory The stored schedules are retained in memory when the AS50 is turned off Section 3 11 describes how to create schedules SCHEDULE EDIT SAVE TO RUN MISSING VIAL ACTION STOP VIAL INJ INJ LINE START END VIAL VOL uL METHOD 1 1 1 25 0 1 Help Prompt Figure 2 10 Schedule Screen Each injection in a schedule is assigned a method a series of operating instructions that tells the AS50 how to perform a single injection A method consists of the following three phases Method Phase Used To Examples Sample prep Prepare the sample for injection Pipetting mixing diluting Method setup Set parameters that remain Sample needle height column constant throughout the method temperature Timed events Perform functions at a specific Loading the sample loop time in the method injecting When the method runs the AS50 performs the sample prep steps first followed by the method setup and then the timed events steps Section 3 10 describes how to create methods 2 18 Doc 031935 01 4 04 2 Description 2 5 3 Locked Remote Direct Control Mode Chromeleon Control Connecting the AS50 to a Chromeleon Control panel immediately selects the Locked
130. he time is 8 35 p m and 50 seconds enter 20 35 50 Press numeric buttons to enter the time directly or press Select A or Select V to increase or decrease the current value DATE Sets the date on the real time clock Press numeric buttons to enter the day and year directly or press Select A or Select V to increase or decrease the current values Press Select A or Select V to select the month Doc 031935 01 4 04 C 23 AutoSelect AS50 Autosampler C 6 5 System Parameters Screen Use the SYSTEM PARAMETERS screen to set default system operating parameters SYSTEM PARAMETERS WAIT ENABLED SYRINGE SPEED 4 CUT SEGMENT VOEUME 310 0 INJ ECT PORT VOLUME 43 uL SRS POWER SWITCHING DISABLED Help Prompt Screen Field WAIT SYRINGE SPEED Figure C 15 System Parameters Screen Description Enables or disables the wait step in the sample prep phase of the method When wait is enabled the 550 waits for a run command from either the front panel Hold Run button or a TTL input signal before performing a method s timed events steps This allows another instrument or Chromeleon to control the timing of each injection in a schedule When wait is disabled a run command is required to start a schedule Thereafter the AS50 performs the schedule lines without requiring additional run commands Disabling wait allows the AS50 to act as a system master The AS50 can send TTL signals to other modules in the system thereb
131. hed The needle arm moves to the flush port and delivers a volume of flush fluid This washes the outside of the needle The flush reservoir and sample syringe are always used to perform the flush The needle is washed with a factory set volume Select the volume of liquid to flush through the inject port on the FLUSH PRIME screen see Section C 7 If you specify a flush volume that is greater than the sample syringe volume the AS50 performs multiple syringe strokes until the volume is reached 2 30 Doc 031935 01 4 04 2 Description Priming The AS50 performs the following operations during priming The sampling needle arm moves to the waste port and expels any fluid from the needle The needle arm then delivers a volume of fluid to the flush port This primes the lines from the flush reservoir to the syringe and through the sample transfer line to the sampling needle see Figure 2 19 When the sample preparation option is installed additional priming is required to prime the lines from the reagent reservoir s to the sample syringe see Figure 2 20 See Section B 3 10 for details Sample Syringe Valve Sample Transfer Line Sample Syringe Sampling Flush Reservoir Needle Figure 2 19 Fluid Schematic for Sample Syringe Doc 031935 01 4 04 2 31 AutoSelect AS50 Autosampler Reagent Holding Loop Front Fitting Back Fitting To From Needle
132. hod The command can be from the front panel pressing Hold Run from Chromeleon or from a TTL or relay input The AS50 has completed overlapped sample prep and is waiting for the timed events of the previous method to complete The AS50 is executing the method setup conditions and the INIT step of the method s timed events The AS50 is waiting for the cycle time to expire before starting the method s timed events The AS50 is waiting for the column and or tray temperature to stabilize before starting the method s timed events The AS50 is executing the method s timed events This status message is displayed until a load or inject step is executed Sample is being loaded into the loop during the method s timed events Sample is being injected during the method s timed events The AS50 is performing operations necessary to abort the schedule Doc 031935 01 4 04 C Display Screens Screen Field VIAL INJ TRAY C COL C TIME VOL Operating Mode LOCAL or LOCKED RMT remote Control Mode SCH schedule or DIRECT CONTROL Description The number of the vial from which the injection is occurring The AS50 updates the vial number after executing the INIT step of the method s timed events The number of injections completed using the current vial on the current schedule line followed by the total number of injections scheduled for this vial If the sample temperature control opti
133. hold the left LED is illuminated After you select a schedule to run pressing Hold Run starts the schedule the right LED is illuminated When the schedule is complete the AS50 returns to hold When running press Hold Run to pause the schedule This button functions only when the 550 is in Local mode Note If the wait operation is enabled see Section 2 6 1 the AS50 pauses when it reaches the wait step in the method Press Hold Run to continue You can disable the wait see Section 2 6 4 Sends the needle arm to its home position This button functions only when the AS50 is in Local mode If a schedule is in progress it must be on hold If you press Home when a schedule is on hold and then resume the schedule the needle arm returns to where it was when you pressed Home When the Door button is pressed a message screen indicates whether it is currently safe to open the door If it is a timer counts down the remaining time in which it is safe to open the door Opening the door without first pressing this button or when the message indicates it is not safe aborts the currently running schedule The following buttons control screen functions 4 Insert NV D Inserts a new step into a method or schedule Move the cursor to the leftmost field on the SAMPLE PREP TIMED EVENTS or SCHEDULE screen and press Insert The new step is added above the cursor position Doc 031935 01 4 04 2 Description
134. hromeleon software Release 6 6 or later The two IC systems can be controlled independently for calibration or simultaneously for unknown sample injections Dual analyses can be performed with only one sample thus increasing sample throughput and eliminating the need to fill track and label two sample vials Because the systems are plumbed in parallel contamination that may result when systems are plumbed in series is prevented In Chromeleon the two IC systems and the AS50 are configured into a single timebase and each system is assigned a unique device name and channel This lets you monitor and control both systems from one Control panel and run all samples in one sequence When the AS50 is equipped with the simultaneous injection option the INSTALLED OPTIONS screen lists two injection valves see Figure E 1 INSTALLED OPTIONS SAMPLE TRAY TEMPERATURE CONTROL COLUMN TEMPERATURE CONTROL SAMPLE PREPARATION INJ ECTION VALVE 2 INJ ECTION VALVE 1 PEEK Help Prompt Figure E 1 Installed Options Screen Simultaneous Injection Option Installed Valves Installed in the AS50 Compartment Doc 031935 01 4 04 E 1 AutoSelect AS50 Autosampler E 1 Injection Valves Optional The injection valves are six port electrically activated Rheodyne valves The valves have two operating positions Load and Inject see Figure E 2 LOAD POSITION INJECT POSITION Sample Ins Sample Ins
135. ide masking tape securing tubing within the recess at the rear of the AS50 6 Remove the masking tape 7 Remove the coiled tubing inside of the recess see Figure 9 Be careful not to damage the tubing Figure 9 Coiled inlet tubing inserted into the recess in the rear of AS50 Doc 031935 01 4 04 F 3 AutoSelect AS50 Autosampler Figure 10 Typical items shipped in Ship Kit geometric foam pack Note All items are in polyethylene bags and wired closed with sturdy ties 8 Remove the items from the geometric foam pack Note For a single syringe a foam insert is placed on the left e Figures and 12 Dual syringes with pigtails bagged and wired closed with sturdy ties Syringes packed with foam insert placed between them Both handles are tied together with sturdy ties 9 Remove the sturdy ties and the polyethylene bag 10 Open the front of the sheath and use the handles to set the pack and syringes on top of the main AS50 11 Slide the syringes into positions see Figure 13 Doc 031935 01 4 04 Unpacking Instructions Figure 13 Fully assembled dual syringes with bottle Figure 14 Shipping bracket hooked into frame and attached to sample arm and chassis with screws 12 Remove the sturdy tie the two cap screws and the bracket see Figure 15 Figure 15 Shipping bracket with M4 Allen cap screws
136. ing guidelines if the sample preparation option is not installed See page 19 if the sample preparation option is installed e See page 20 for the priming procedure Guidelines for AS50s Without the Sample Preparation Option The priming procedure primes the flush reservoir line to the sampling valve and the sample transfer line from the valve to the sampling needle see Figure B 13 The priming volume required depends on the size of sampling needle installed See the table below Sample Syringe Size Suggested Priming Volume lt 1000 pL 2000 to 2500 uL 5000 pL 10 000 uL 10000 uL 20 000 uL Sample Syringe Valve Sample Transfer Line Sample Syringe Sampling Flush Reservoir Needle Figure B 13 Sample Syringe Fluid Schematic System without the Sample Preparation Option B 18 Doc 031935 01 4 04 B Installation Reagent B Optional Guidelines for AS50s with the Sample Preparation Option The priming procedure consists of two parts 1 Prime the flush reservoir line reagent holding loop and sample transfer line see Figure B 14 Reservoir Syringe Syringe TotalSuggested Number of Priming Size Priming Volume Cycles Required FLUSH PRIME 25mL 5000 uL 1 5 mL 10 000 uL 2 10 mL 20 000 uL 4 For each priming cycle enter a PRIME VOLUME of 5000 uL 2 Prime the lines from each installed reagent reservoir Reservoir Syringe Syringe Size Suggested Priming Volume A
137. injection if any of the following events occurs WAIT FOR TEMP STABLE is enabled and the column temperature did not stabilize by the end of the cycle time The sample prep wait operation is enabled and a run command did not occur by the end of the cycle time The previous method s timed events extend beyond the cycle time If the cycle time expires before the injection occurs a warning message is displayed and the message is logged on the MESSAGE LOG screen see Section C 9 6 2 26 Doc 031935 01 4 04 2 Description 2 6 6 Operating Events During a Schedule Figure 2 17 shows the order of events during a schedule when the wait operation is enabled Figure 2 18 shows the order of events during a schedule when the wait operation is disabled See Section 2 6 1 for details about the wait operation Doc 031935 01 4 04 2 27 AutoSelect AS50 Autosampler Schedule Started 4 Preparing to Run Flush Inject Port and Wash Needle 4 Method Starts for Injection 1 4 Flush Inject Port and Wash Needle See Note 1 No Yes T Yes lt Temperature s Wait for No Temperature to Stabilize Method Starts for Injection 2 4 Flush Inject Port and Wash Needle See Note 1 e No Vial Present Sample Prep Overlap See Note 2 Do Sample Prep Pipetting Mixing etc 4
138. ion in the AS50 Properties dialog box in the Server Configuration program determines whether samples are overlapped Sample overlap is enabled by default in Chromeleon For instructions on how to configure the AS50 in Chromeleon see Section B 3 9 Doc 031935 01 4 04 2 23 AutoSelect AS50 Autosampler 2 6 3 Running Samples Sequentially Placing the wait at the start of the sample prep steps allows samples to be run sequentially The AS50 completes the sample prep and timed events for an injection before starting the next see Figure 2 14 Start First Method Start Next Method WASH SAMPLE PREP ISETUPIINIT TIMED EVENTS WASH WAITISAMPLE PREP SETUP INIT TIMED EVENTS WASH FLUSH FLUSH FLUSH Run Continue Continue Figure 2 14 No Sample Prep Overlap Wait Operation at Beginning of Sample Prep Phase 2 24 Doc 031935 01 4 04 2 Description 2 6 4 Using the 550 as the System Master Disabling the Wait Operation When the wait operation is disabled a single run command for example pressing the Hold Run button starts the schedule Thereafter the AS50 performs the schedule lines without requiring additional commands This allows the AS50 to act as the system master the AS50 controls the system by sending TTL signals to start the pump and detector methods To disable the wait operation press Menu and 5 to go to the MODULE SETUP MENU and press 5 to go to the SYSTEM PARAMETERS screen Set th
139. isting white Teflon washer from inside the valve port and replace it with a new washer P N 061315 The washers are supplied with the syringe valve and with the AS50 Preventive Maintenance Kit Reinstall the syringe and valve Make sure the syringe is tightened To tighten hold the syringe at the top and bottom fittings and turn it clockwise as viewed from the top see Figure 4 1 Tighten fingertight only do not overtighten wl Figure 4 1 Tightening the Syringe Doc 031935 01 4 04 4 21 AutoSelect AS50 Autosampler 4 2 2 4 2 3 4 2 4 4 2 5 Leaking Drain Line Connection Make sure the drain line is not crimped or otherwise blocked Make sure the line is not submerged in liquid in the waste container Empty the container if needed e For autosamplers without sample temperature control make sure the line is pushed tightly onto the drip tray fitting and that it is not elevated at any point after it exits the autosampler For autosamplers with sample temperature control Leaking Inject Port Check for a leaking fitting and tighten Check for a damaged or worn needle seal Replace the needle seal assembly if necessary see Section 5 6 If fluid appears on the top of the inject port during injection the needle may not be reaching the correct depth in the port or may not be aligned over the inject port Try aligning the injec
140. itting off the end of the tubing and remove the bolt Tape one end of a piece of string or tubing to the end of the sample transfer line This will help you thread the new line through the autosampler later in the procedure Syringe Valve To Port C on Sample Clamp Bracket 2nd Yellow Sleeve Bd uw Sample Note This coupler is Transfer included on simultaneous Line injection models only Right Side of Sampling Autosampler Needle Compartment Figure 5 5 Sample Transfer Line Bracket and Clamp Right Side of Compartment Doc 031935 01 4 04 5 9 AutoSelect AS50 Autosampler 11 From the inside of the autosampler pull the tubing into the autosampler compartment along with the end of the attached string or tubing 12 Turn the needle fitting above the needle block see Figure 5 6 counterclockwise until it is loose 13 Pull the needle assembly up and out of the needle block and cradle 14 Remove the needle assembly from the autosampler Figure 5 6 Removing the Needle from the Cradle 5 10 Doc 031935 01 4 04 5 Service 5 3 2 Installing the New Sampling Needle Assembly 1 Figure 5 7 illustrates the sampling needle assembly Yellow Sleeves Sample Transfer Line Tubing S o A Ferrule Needle Bolt Shipped unattached Figure 5 7 Sampling Needle Assembly 2
141. ix operation 3 23 Needle operation 3 22 Overlap 2 22 2 23 2 33 Pipet operation 3 23 Running sequentially 2 24 Wait step 2 22 Sample Prep screen C 10 Example 3 20 Sample prep syringe See Prep syringe Sample preparation option 2 9 Diluting 3 25 C 12 Dispensing reagent C 11 Dispensing reagents 3 24 Example method 3 32 Fluid schematic 2 32 Functions 2 9 Priming B 19 Setup B 7 Sample syringe Fluid schematic 2 31 B 18 Functions of 2 9 Specifying the volume of B 14 C 21 Volumes of 2 9 Sample temperature control option 2 2 2 12 Installation B 5 Specifications A 3 Tray covers for 3 2 Tray for 2 11 Sample tray See Tray Sample viscosity 3 45 Sampler door opened 4 13 Sampling needle 2 10 Aligning 5 19 Replacing 5 8 Sampling needle arm 2 10 Sampling process Description of 2 10 Saving a method 3 17 Saving a schedule 3 34 Schedule control mode 2 16 2 17 Selecting C 5 Schedule error Schedule does not exist 4 8 Schedule lines Defining 3 35 Schedule screen 2 18 C 17 Schedule steps 3 35 Doc 031935 01 4 04 Index 9 AutoSelect AS50 Autosampler Schedules Copying 3 37 Creating 3 33 Deleting 3 37 Deleting steps 3 35 Editing 3 37 Editing a running schedule 3 8 Inserting steps 3 35 Number allowed 3 8 Pausing 2 4 Resuming 2 4 Running from the front panel 3 8 Saving 3 34 Selecting 3 9 Skipping a missing vial 3 34 Starting 3 9 Stopping 2 4 3 11 Schemati
142. k of Dionex Corporation Teflon and Tefzel are registered trademarks of E I duPont de Nemours amp Co Windows is a registered trademark of Microsoft Corporation PRINTING HISTORY Revision 01 April 2004 Contents 1 Introduction 1 1 1 2 OVerVIQGW ERE ecient a area aa EA UR T a age a 1 1 About This Manual eeeeeeeee 1 2 1 2 1 Safety Messages and Notes 1 3 1 2 2 Safety Labels 4 Le ET Ies 1 5 2 Description 2 1 Overview of Features 0 0 eee eee eh 2 1 2 2 Front Panel Features cscri iot rerea eee ees 2 3 2 2 1 Keypad Button Functions 2 4 2 2 2 Display Screens 2 6 2 3 Autosampler Compartment 2 8 2 3 1 Syringe and Reservoir Organizer 2 9 2 3 3 Sampling Needle Arm 2 10 2 3 3 Flush Waste and Inject Ports 2 11 2 3 4 Sample Vials and Trays 2 11 2 3 5 Sample Temperature Control Option 2 12 2 3 6 Simultaneous Injection Option 2 13 2 4 Rear Panel Features i eme eee hae Eam aie es 2 14 24 1 RS 232ConnectOr uere IRR EE 2 14 242 USB Receptacle ilc Roos poe eyes 2 15 Doc 031935 01 4 04 i AutoSelect AS50 Autosampler 2 5 2 6 243 TTL Relay Co
143. l calibration range 4 15 Cursor arrow buttons 2 5 Cut segment volume Definition 3 42 Example 3 43 Range allowed 3 38 Specifying for partial loop injections 3 45 C 25 Cycle time Example 2 26 Expiring 2 26 Overview of 2 26 Specifying in the method 3 27 C 13 Cycle time expired 4 10 D Daily maintenance 3 46 Date Setting B 15 C 23 Decibel level A 1 Default field values 2 5 Default operating parameters 3 6 Defining sample prep steps 3 18 3 19 Defining schedule lines 3 35 Delay between sample prep steps 3 18 3 22 C 10 Delay of injections 2 26 3 28 Delete button 2 5 Deleting Lines in a method 2 5 Methods 2 5 3 31 Sample prep steps 3 20 Schedules 2 5 3 37 Steps in a schedule 3 35 Values from screen fields 2 5 Detail Status screen 2 17 C 6 Example 2 33 Devices Assigning unique names to E 10 Diagnostic Menu C 31 Diagnostic screens Code Versions C 43 Diagnostic Tests C 32 Leak Sensor Calibration and Status C 41 Liquid Control C 37 Logs menu C 39 Power Up C 31 Temperature Statistics C 35 XYZ Test C 33 Diagnostic tests At power up 3 3 B 13 Index 2 Doc 031935 01 4 04 Index Diagnostic Tests screen C 32 Dilutions 2 10 3 18 3 25 C 12 Dimensions AS50 autosampler compartment 1 Dionex Contacting 5 1 Direct control mode 2 16 Description of 2 17 Selecting 3 14 C 5 With Chromeleon 2 19 Disabling the wait operation 2 25 3 21 Dispensing r
144. l conditions are executed immediately after the method setup second step is the time zero step which is executed after the INIT step When the time zero step is executed the timed events clock begins counting up from zero and any additional timed events run at their specified times NOTE When a cycle time is specified the AS50 delays executing the time zero step until the specified time has elapsed since the previous injection see Section 2 6 5 for details Adding Timed Events Steps Useone of the following methods to add a step Move the cursor to the empty TIME field below the last step Enter the elapsed time at which to start the new step and press Enter or a cursor arrow button e Move the cursor to any TIME field and press Insert This adds new step before the cursor position Enter the elapsed time at which to start the new step and press Enter or a cursor arrow button After you add a step the steps are reorganized in chronological order 2 Enterthe remaining parameters for the new step see the following sections To retain a value set in the previous step leave the field blank A blank field means there is no change from the previous step Deleting Timed Events Steps To delete a step move the cursor to the step s TIME field and press Delete twice Doc 031935 01 4 04 3 29 AutoSelect AS50 Autosampler Controlling the Injection Valve The VALVE parameter sets the position of the inj
145. laced see Section 5 11 e Ifthe syringe does not move in response to a command the cable may need to be replaced Contact Dionex Doc 031935 01 4 04 4 Troubleshooting 378 Time out occurred waiting for syringe to respond to command Possible Causes Communication problem Actions Turn off the AS50 power check the syringe cable connections and then turn on the power again If the message reappears contact Dionex 380 Response from syringe was too long Receive buffer overflowed Possible Causes Communication problem Actions Turn off the AS50 power and then turn on the power again If the message reappears contact Dionex 381 Anattempt was made to move the syringe to an illegal position Most likely the specified volume was larger than the remaining syringe capacity Valid positions for the syringes are programmed into the AS50 instrument control Moduleware There was an internal instruction to move a syringe to a position that was not programmed Possible Causes Problem with the Moduleware Actions Turn off the 550 power and then turn on the power again If the message reappears contact Dionex e 382 An attempt was made to move a syringe valve to an illegal position Valid positions for the syringe valves are programmed into the AS50 instrument control Moduleware There was an internal instruction to move a syringe valve to a position that was not programmed Possible Causes Prob
146. lect AS50 Autosampler 2 4 Rear Panel Features Thermal or Chromatography Compartment Cable Connector not used RS 232 Printer Connector USB Receptacle TTL Relay Q Connectors Syringe 9 Connectors Main Power Q Receptacle and Fuse Holder Auxiliary 7 Power Connector 2 4 1 Figure 2 8 AS50 Rear Panel RS 232 Connector The RS 232 connector allows a serial printer to be connected to the AS50 for printing of schedules methods setup and configuration information or the message log 2 14 Doc 031935 01 4 04 2 Description 2 4 2 2 4 3 USB Receptacle The USB receptacle provides a connection to the Chromeleon computer To enable computer control of the autosampler connect a USB cable between the USB receptacle on the AS50 rear panel and a USB port on the computer or USB hub See Section B 3 5 for USB connection instructions TTL Relay Connectors The eight relay and TTL connectors interface with Dionex and non Dionex modules The input connectors allow for relay and TTL control of the autosampler by a connected module s The output connectors allow the AS50 to control other module s Appendix D describes the relay and TTL functions and the connections between the AS50 and other modules Doc 031935 01 4 04 2 15 AutoSelect AS50 Autosampler 2 5 Operating and Control Modes The operating mode determines how 550 receives operating commands
147. lem with the Moduleware Actions Contact Dionex Doc 031935 01 4 04 4 5 AutoSelect AS50 Autosampler 383 384 386 An attempt was made to set the syringe speed to an illegal value Valid speeds for the syringes are programmed into the AS50 instrument control Moduleware There was an internal instruction to move a syringe at a speed that was not programmed Possible Causes Problem with the Moduleware Actions Contact Dionex Cannot use sample syringe to aspirate from specified source reservoir Possible Causes The sample syringe can aspirate only from the flush reservoir or from the sampling needle Actions Select a different source for the aspirate function or select the prep syringe if installed Operation could not be completed because vial tray is not installed Magnets on the vial trays indicate to a magnetic sensor in the autosampler compartment when a tray is installed and the type of tray Possible Causes assuming a tray is installed but is not recognized by the sensor Tray magnets are missing or incorrectly installed e Sensors are not working Actions Replace the tray Replace the tray sensor card Contact Dionex Doc 031935 01 4 04 4 Troubleshooting 387 An illegal vial number was specified The type of tray installed determines which vial numbers can be used in methods and schedules Tray Type Tray Capacity 1 5 mL aluminum 100 vials 1 5
148. librate see Section 5 10 Leak sensor needs recalibration Go to Leak Sensor Calibration screen to perform recalibration operation Actions See Section 5 10 for calibration instructions Motion command to DSP was aborted Needle may not have homed properly Possible Causes Loose coupling in the Z axis The needle does not stay up Actions Try the motion again If the problem persists contact Dionex Recoverable motion error occurred in Y axis Possible Causes An error occurred during Y axis movement of the sampling arm The DSP was able to recover from the error and continue the motion Actions Occasional occurrences of this error can be ignored If the problem persists contact Dionex Doc 031935 01 4 04 4 15 AutoSelect AS50 Autosampler 450 Non recoverable motion error occurred in Y axis Clear any 451 physical obstructions and then press any key to reset system Possible Causes Vial obstructing motion Misaligned sampling needle arm Actions Remove the vial obstruction Align the sampling needle arm in the inject port see Section 5 7 Turn off the power and check for freedom of movement of all three axes of the sampling arm Recoverable motion error occurred in Z axis Possible Causes An error occurred during Z axis movement of the sampling arm The DSP was able to recover from the error and continue the motion Actions Occasional occurrences of this error can be ignored I
149. llowing options SYRINGE SPEED 5 ACTION EMPTY Press Enter Select the following options from FLUSH SYRINGE SPEED 5 ACTION FILL Press Enter Select the following options DISPENSE 50 uL SYRINGE SPEED 5 ACTION DISPENSE Press Enter Select VIAL INJ and press Enter The needle arm moves to the inject port Select the following options DISPENSE of key presses microliters recorded in Step 19 SYRINGE SPEED 3 ACTION DISPENSE Press Enter Verify that a drop of liquid appears at the end of the needle seal tubing If no drop appears or if the size of the drop is different repeat Step 7 through 32 Select VIAL FLU and press Enter Select the following options SYRINGE SPEED 5 ACTION EMPTY Press Enter Doc 031935 01 4 04 5 25 AutoSelect AS50 Autosampler 36 37 38 39 40 41 42 Press Home to move the needle arm to the home position Press Menu twice to go to the MENU of SCREENS Press 5 twice to go to the SYSTEM PARAMETERS screen SYSTEM PARAMETERS WAIT ENABLED SYRINGE SPEED SA CUT SEGMENT VOLUME 10 uL INJECT PORT VOLUME 43 uL SRS POWER SWITCHING DISABLED Help Prompt Figure 5 20 System Parameters Screen In the INJECT PORT VOLUME field enter the number of key presses microliters recorded in Step 19 NOTE You can enter the inject port volume on the Chromeleon Wellness panel instead of on the SYSTEM PARAMETERS screen see Section 5 15 Pre
150. ls for various IC system configurations To open a Control panel expand the folder for the type of system to which the AS50 is connected for example Dionex_IC ICS 2000 System Panels and double click a panel that includes an AS50 For Doc 031935 01 4 04 2 19 AutoSelect AS50 Autosampler example double clicking ICS 2000_System_AS50 pan opens the Control panel shown in Figure 2 11 After opening the Control panel connect it to the timebase in which the AS50 is configured select Control Connnect to Timebase Km ICS 2000 System with Column Heater Eluent Generator and 4550 AutoSampler Test AMAR System Log Audit Trail on Te 11 36 35 User AMar from AMAR has acquired control over timebase Test ICS 2000 Pum Sample mu Datasource E Sequence Sample Pump Settings rmm ICS 2000 Eluent Generator Cartridge Elapsed Program Time Total Program Time System Connected Startup Shutdo EXER EG Settings i ICS 2000 Injector and 550 AutoSampler si LoadPosiion 80 Sampler Settings ICS 2000 Column Heater 20 0 pl from Pos 1 Temperature Heater Settings ICS 2000 Detector 0 049 uS Suppressor 1 0 Detector Settings 0 00 0 50 1 00 1 50
151. lumn suppressor etc before installing the autosampler Before connecting the AS50 to a Chromeleon PC make sure Chromeleon is installed on the PC Refer to Installing the Chromeleon Chromatography Management System with a Dionex Ion Chromatograph Document No 031883 for instructions B 3 2 Installation Checklist Oooovoovoo Connect the syringe s and drain line Connect the injection valve Optional Connect the AS50 to the Chromeleon PC Plug the power cord into a grounded power source Turn on the system power Select the module setup options Prime the liquid lines Remove bubbles from the syringe Doc 031935 01 4 04 B 3 AutoSelect AS50 Autosampler B 3 3 Connecting the Syringe s and Drain Line 1 Remove the acorn nut from the fitting on the sample transfer line which extends from the AS50 rear panel Gently pull the line out the rear panel until there is enough free tubing about 45 cm 18 in to route the line to the front of the sample syringe Remove the 1 4 28 plug from port C on the sample syringe valve Make sure the white Teflon washer remains installed inside the port Connect the sample transfer line to port C see Figure B 1 Flush Reservoir Sample Syringe Figure B 1 Syringe Connections Fill the flush reservoir P N 057408 with flush fluid normally deionized water and place it in the organizer Remove the 1 4 28 plug from port E on the sample syringe Make su
152. m which to dispense the reagent RES A RES B RES C or RES D default RES A to Enter the destination vial position the vial to receive the dispensed volume default SMP Doc 031935 01 4 04 C 11 AutoSelect AS50 Autosampler Prep Operation Description DILUTE Dilutes a volume of concentrate with a specified volume of diluent DILUTE is available only if the sample preparation option is installed uL Enter the concentrate volume from 1 0 to 99 9 uL or 100 to 1000 mL default 10 uL Enter the source vial The sample syringe picks up the concentrate from the source vial and dispenses it to the destination vial default vial S 1 uL Enter the diluent volume default 1000 uL source Select the reservoir from which to dispense the diluent RES A RES B RES C or RES D default RES A to Enter the destination vial position the vial to receive the concentrate and diluent volumes default vial SMP WAIT The AS50 waits for a continue command before proceeding with the method The command can be from the front panel pressing Hold Run or a TTL input Each method must have one wait step and no more Sample prep operations can be added before and after the wait step By default the wait step is at the end of the sample prep phase The wait step can be disabled from the SYSTEM PARAMETERS screen see Section C 6 5 C 12 Doc 031935 01 4 04 C Display Screens C 4 2 Method Setup Screen Use
153. n The tubing must curve away from the arm assembly when the arm is moved toward the right front of the autosampler Adjust the tubing length to the needle when the needle is all the way down 8 10 11 Close the autosampler door and turn on the power Press Home to confirm the tubing moves correctly Turn off the power and open the door Position the second yellow sleeve on the tubing in the position measured in Section 5 3 1 Step 7 Loop the tubing at the second yellow sleeve and attach it to the center bracket on the upper right side of the autosampler compartment see Figure 5 5 Use a screwdriver to push in the bracket slightly and secure the tubing NOTE The front and back brackets on the upper right side of the compartment are not used Loop the remaining tubing and hang it on the rear clamp leaving about 45 cm 18 in free 5 12 Doc 031935 01 4 04 5 Service 12 13 Tape the string or tubing that was pulled into the compartment in Section 5 3 1 Step 11 to the free end of the new tubing Pull the string or tubing along with the new tubing out the back of the compartment Slide the bolt large end first onto the end of the tubing and then slide the ferrule small end first onto the tubing see Figure 5 9 Slide the ferrule about 10 mm 3 8 in past the end of the tubing Tubing Bolt f Ferrule 14 15 16 17 Figure 5 9 Installi
154. n Field ASPIRATE from DISPENSE SYRINGE ACTION NEEDLE HEIGHT SYRINGE SPEED INJ VALVE CS VALVE INJ VLV 1 INJ VLV 2 simultaneous injection mode Description The volume of liquid to be drawn during an aspirate action The source for the action The choices depend on the syringe in use Sample NEEDLE FLUSH Prep NEEDLE FLUSH RES A RES B RES C RES D AIR The volume of liquid to dispense during a dispense action The syringe to use for an action The action to perform Press Select A or Select V to cycle through the choices press Enter to start the action The height of the needle above the bottom of the vial If 0 is selected a small distance is added to prevent the needle from touching the bottom of the vial The syringe speed to use Use a value of 5 for water More viscous liquids require slower speeds The injection valve position The position of the optional column switching valve Note The column switching valve option is not currently available for the AS50 USB If the AS50 is equipped with two injection valves for simultaneous injections these fields select the position of each valve Injection valve 1 is the bottom valve and injection valve 2 is the top valve Values 1 0 to 99 9 uL or 100 to 1000 uL default 100 uL 1 0 to 99 9 uL or 100 to 1000 uL default 100 uL SAMPLE PREP ASPIRATE DISPENSE EMPTY FILL 0 to 46 mm default 46 mm 1
155. n the inject port and push the needle seal into the inject port 8 Rotate the bottom fitting until the slots are perpendicular to the projections on the inject port see Figure 5 17 Inject Port Projection Slot Figure 5 17 Inject Port 9 Reinstall the drip tray see Section 5 5 5 18 Doc 031935 01 4 04 5 Service 10 Align the sampling needle in the inject port see Section 5 7 and then calibrate the inject port volume see Section 5 8 NOTE Do not connect the needle seal line to the injection valve until after aligning the inject port and calibrating the inject port volume 5 7 Aligning the Sampling Needle in the Inject Port Align the sampling needle in the inject port after replacing the sampling needle assembly and or the needle seal assembly or if the needle becomes misaligned after a period of operation NOTE Before aligning the sampling needle in the inject port always inspect the needle to make sure it is not bent If the needle is bent replace the sampling needle assembly see Section 5 3 NOTE This procedure has numerous steps To help maintain your place in the procedure check off each step after completing it 1 Close the autosampler door and turn on the power 2 Forabetter view of the needle and inject port during the alignment procedure you can temporarily disable the door lock to allow operation with the door open a From the MENU of SCREENS press 5 and then 7
156. nex 457 474 Syringe reports fatal error 475 Syringe reports error Possible Causes Syringe errors can result from various mechanical problems or from a communication failure between the syringe and the AS50 electronics Actions Check the syringe cable connections For fatal errors press a key to reset the system e Ifthe error persists contact Dionex Cannot run schedule No DSP detected Possible Causes Electronic malfunction Actions Turn off the power and then turn on the power again If the message reappears contact Dionex 4 18 Doc 031935 01 4 04 4 Troubleshooting 476 479 Cannot run schedule No sample syringe detected Possible Causes e Syringe cable not plugged into the rear of AS50 Faulty syringe cable or syringe Actions Check the syringe cable connection Replace the cable Contact Dionex Non recoverable error Both internal and external inject valves are connected Please power down and disconnect one inject valve before continuing Possible Causes An injection valve is installed in both the autosampler compartment and in another module Actions Disconnect one of the valves Doc 031935 01 4 04 4 19 AutoSelect AS50 Autosampler 480 Timed out waiting for tray temperature to stabilize Either temperature control is broken or set temperature is too far away from ambient The sample temperature control option was no
157. ng screw mount on the syringe drive Doc 031935 01 4 04 B 21 AutoSelect AS50 Autosampler 11 Insert the syringe drive connecting screw removed in Step 2 and tighten fingertight 12 Perform a flush cycle a Press Menu Menu and 6 to go to the FLUSH PRIME screen b Move the cursor to the ACTION field and press Select A or Select V to select FLUSH Press Enter B 22 Doc 031935 01 4 04 C Display Screens This appendix illustrates and describes the screens available for display on the front panel of the 550 Figure C 1 illustrates the hierarchy of screens MAIN 2 DETAIL SCHEDULE STATUS STATUS MENU 1 SAMPLE PREP 2 METHOD SETUP TIMED EVENTS MENU of SCREENS MODULE 6 FLUSH SETUP MENU PRIME 1 INSTALLED OPTIONS FRONT PANEL 3 PLUMBING CONFIGURATION 4 5 system PARAMETERS 6 IN ECT PORT ALIGNMENT 7 INTERLOCK BYPASS 7 qme pmewosrc print FUNCTION IN MENU MENU FUNCTION OUT 1 POWER UP 1 PRINT SCHEDULE 2 DIAGNOSTIC TESTS 2 PRINT METHOD 3 XYZ TEST 4 TEMP Available only STATISTICS with the sample temperature option 5 LIQUID CONTROL 6 106 MENU 1 USAGE LOG 7 LEAK CAL amp STATUS 2 MESSAGE LOG 8 KEYBOARD TEST 9 VERSIONS Figure C 1 AS50 Menu Structure Doc 031935 01 4 04 AutoSelect AS50 Autosampler C 1 Menu of Screens Pressing M
158. ng the Ferrule Fitting and Bolt Insert the tubing with the bolt and ferrule into port C on the sample syringe Push the tubing snugly into the bottom of the port Close the autosampler door and turn on the power As the arm moves to the idle position verify there is enough slack in the sample transfer line to prevent the tubing from being pulled Turn off the power and open the door While holding the tubing with one hand to avoid twisting it screw the bolt into the port fingertight This seats the ferrule onto the tubing If the fitting leaks when operation is resumed tighten the bolt slightly Do not overtighten Overtightening can damage the inside of the valve 18 Realign the new sampling needle in the inject port See Section 5 7 for instructions Doc 031935 01 4 04 5 13 AutoSelect AS50 Autosampler 5 4 Removing the Drip Tray NOTE This section is provided as a reference when performing service procedures that require removing the drip tray Using a 2 5 mm Allen wrench remove the screw that secures the drip tray autosampler compartment and remove the injection valve waste line from the front of the tray see Figure 5 10 Pull out the tray toward the front of the autosampler about 6 mm 1 4 in Tilt the bottom of the tray out slightly and push the tray down slightly until the leak sensor on the inside of the tray see Figure 5 10 clears the overhang on the front of the autosampler Pull the tray ou
159. nnectors 2 15 Operating and Control Modes 2 16 2 5 Local Direct Control Mode 2 17 2 5 2 Local Schedule Control Mode 2 17 2 5 3 Locked Remote Direct Control Mode Chromeleon Control e BE Se Sedo hU PU ASHER ee 2 19 Theory of Operation 2 22 2 6 1 Understanding the Wait Operation 2 22 2 6 2 Overlapping Sample 2 23 2 6 3 Running Samples Sequentially 2 24 2 6 4 Using the AS50 as the System Master Disabling the Wait Operation iceri eror niire eR aS tee ner RR dex a 2 25 2 6 5 Understanding the Cycle Time 2 26 2 6 6 Operating Events During a Schedule 2 27 2 6 7 Operating Events During Flushing and Priming 2 30 2 6 8 Understanding the Status Display During aRun 2 33 3 Operation and Maintenance 3 1 3 2 3 3 3 4 3 5 Getting Ready to 3 1 3 1 1 the Vials and Load the Sample Tray 3 1 3 1 2 Turn On the Power 3 3 Overview of 50 3 4 Default Operating Parameters 3 6 Selecting Computer or Front Panel Local Control 3 7 Running a Schedule of Inj
160. ns a liquid crystal display LCD and membrane keypad see Section 2 2 The front panel permits manual control of all AS50 functions Autosampler Compartment The autosampler compartment contains a stationary sample vial tray and a sampling needle arm that moves from vial to vial during operation see Section 2 3 Syringe and Reservoir Organizer The organizer on top of the autosampler holds the sample syringe the flush liquid reservoir the optional prep syringe and the optional reagent reservoir s See Section 2 3 1 for details Options Not Pictured Sample Temperature Control The optional sample temperature control unit mounts under the autosampler and provides uniform heating and cooling of the vial tray See Section 2 3 5 for details Simultaneous Injection The simultaneous injection option lets a single AS50 deliver sample to two independent ion chromatography IC systems See Appendix E for details Injection Valve The AS50 connects to an injection valve installed in another chromatography system module Commands from the AS50 front panel or from Chromeleon are used to control injection valve operation With the simultaneous injection option the AS50 connects to two injection valves installed in two ion chromatography system modules or in the AS50 autosampler compartment 2 2 Doc 031935 01 4 04 2 Description 2 2 Front Panel Features AS50 Autosampler IDLE VIAL 10 TIME 99 9 DIO
161. nsert 2 4 Menu 2 5 Numeric 2 5 Select 2 5 Stop 2 4 C Cables Syringe B 6 USB B 10 Calibration Inject port volume 5 23 Leak sensor 5 27 C 41 Calibration standards 2 10 Canceling an entry 2 5 Cannot run schedule No DSP detected 4 18 Cannot run schedule No sample syringe detected 4 19 Cap 3 2 Cation E 15 Changing values in screen fields 3 5 Choosing the injection type 3 38 Chromeleon 1 1 2 16 Connecting to a Control panel B 17 Connecting to a timebase B 17 Control panel description 2 19 2 20 Locked remote direct control 2 19 Menu 2 19 Method E 14 Doc 031935 01 4 04 Index 1 AutoSelect AS50 Autosampler Programs 2 19 Setting up simultaneous injections E 10 Toolbars 2 19 Chromeleon Server Starting B 17 Clock Display during a run 2 33 During schedule hold 3 10 Starting timed events 3 29 Code Versions screen C 43 Column switching valve 3 15 Commands Chromeleon 2 19 Computer control 1 1 2 15 Selecting 3 7 Connectors Rear panel 2 15 Contacting Dionex 5 1 Control modes 2 16 Direct 2 17 3 14 Schedule 2 16 2 17 Selecting C 5 Control panel 2 19 See Front panel Wellness panel 2 19 Control panel Chromeleon 2 19 For simultaneous injections E 10 System panel 2 19 Cooling the sample tray 2 2 2 12 Copying a method 3 32 Copying a schedule 3 37 Creating a method 3 16 3 17 Creating a schedule 3 33 CS VLV 3 15 Current leak sensor voltage is outside of lega
162. nt The washers are supplied with the valve and with the 550 Preventive Maintenance Kit Holding the syringe at the top fitting screw it into the bottom of the valve at port D and tighten fingertight With valve ports A and D at the 12 o clock and 6 o clock positions respectively push the valve onto the syringe drive shaft Replace the two valve thumbscrews and partially tighten Carefully align the syringe piston with the connecting screw mount on the syringe drive Insert the syringe drive connecting screw and tighten fingertight Finish tightening the valve thumb screws Reconnect the valve liquid lines Tighten the fittings fingertight IMPORTANT If a fitting leaks after operation is resumed tighten it slightly Do not 12 13 overtighten overtightening can damage the inside of the valve From the MENU of SCREENS press 5 and 3 to go to the PLUMBING CONFIGURATION screen Set the syringe home position see Section 5 2 5 On the PLUMBING CONFIGURATION screen move the cursor to the SAMPLE SYRINGE VOLUME field Press Select A or Select V to select the syringe volume Press Enter 5 30 Doc 031935 01 4 04 5 Service 5 13 Replacing the Prep Syringe Valve 1 Disconnect the liquid lines from the existing valve 2 Remove the existing valve see Section 5 11 3 When the valve is removed the syringe drive shaft is visible When the syringe is in the initial position the position selecte
163. o the LIQUID CONTROL screen Select VIAL INJ and press Enter The needle moves into the inject port Position the cursor in the FROM field and select FLUSH Doc 031935 01 4 04 5 21 AutoSelect AS50 Autosampler 22 23 24 25 26 27 28 29 30 31 32 Position the cursor in the SYRINGE field and select SAMPLE Position the cursor in the ACTION field and select FILL Press Enter The syringe fills Select SYRINGE SPEED 5 This speed ensures a fast enough flow rate to detect a leak Select ACTION EMPTY and press Enter Repeat the FILL and EMPTY actions until the new needle seal tubing is filled Once the tubing is filled and fluid reaches the needle empty the syringe again and monitor the top of the inject port for fluid If fluid appears first wipe up the leak and then adjust the needle s Z position down see the next step To adjust the needle s Z position a Press Menu Menu 5 and 6 to return to the INJECTION PORT ALIGNMENT screen b Increase the Z ADDRESS field value 5 steps and press Enter c Repeat Step 17 through 26 above and check again for leaks d Ifaleak still occurs continue increasing the Z address in 5 step increments checking for leaks after each increase until no more leaks occur NOTE Increase the Z address only until no more leaks occur If the Z address is too large the needle will hit the bottom of the port during operation and can damage the needle
164. od definition always includes the parameters from all three of the screens Notes The method phases are run in the order listed above See Section 2 5 2 for details e method can have a total of 100 steps including the sample prep steps the method setup which counts as one step and the timed events steps You can create a new method when the AS50 is running on hold or stopped 3 16 Doc 031935 01 4 04 3 Operation and Maintenance 3 10 1 Creating a Method 1 Press Menu and 3 to go to the METHOD MENU screen see Figure 3 12 METHOD MENU EDIT 25 SAVE 25 1 S AMIPISESPIRIEIP 2 3 TIMED EVENTS Help Prompt 5 Figure 3 12 Method Menu In the EDIT field enter the number of an unused method Press Enter Go to each method screen and enter the method parameters For details about defining sample prep steps see Section 3 10 3 For details about selecting method setup parameters see Section 3 10 4 For details about defining timed events see Section 3 10 5 Save the method see Section 3 10 2 3 10 2 Saving a Method 1 From any of the three method screens press the Menu button to return to the METHOD MENU Position the cursor in the SAVE TO field Do one of the following e To save the current method press Enter e To save to a different method enter a new number and then press Enter Doc 031935 01 4 04 3 17 AutoSelect AS50 Autosampler
165. on see Figure 5 3 View A aN View A Initial he Position Home du Position Set Home Thumbwheel View B Figure 5 3 Initializing the Syringe and Setting the Home Position 5 6 Doc 031935 01 4 04 5 Service 5 2 5 Setting the Syringe Home Position 1 Move the set home thumbwheel to the left to raise the syringe plunger until it is approximately 1 to 2 mm from the top of the syringe see Figure 5 3 View B When the syringe is in operation this set position will be the uppermost stopping point for the syringe plunger Move the cursor to the SAMPLE SYRINGE CONFIG field or the PREP SYRINGE CONFIG field on the PLUMBING CONFIGURATION screen Press Select V to select the SET HOME position Press Enter The syringe moves down to the initial position and then back up to the home position Doc 031935 01 4 04 5 7 AutoSelect AS50 Autosampler 5 3 Replacing the Sampling Needle Assembly 5 3 1 Removing the Old Sampling Needle Assembly 1 Press Menu 8 and 5 to go to the LIQUID CONTROL screen 2 With the cursor in the VIAL field press Select V to select FLU and press Enter The needle arm moves to the flush port 3 Turn off the power and open the autosampler door 4 The sample transfer line tubing that connects the sampling needle to the sam
166. on is installed this field displays the current tray temperature A symbol to the right of this field indicates the temperature status see the description in COL C below If the sample temperature control option is not installed the field displays and it is not backlighted This field is for support of the thermal compartment option which is not currently available The number of minutes since the last injection The clock counts up until the next injection begins or until reaching the maximum time of 999 9 minutes The volume of sample to be injected Select LOCAL mode for control from the front panel When the AS50 is connected to Chromeleon software connecting the AS50 to a Chromeleon Control panel automatically selects LOCKED RMT In Locked Remote mode the front panel keypad is disabled to prevent any changes to operating parameters To return the 550 to Local mode disconnect the AS50 from the Chromeleon Control panel or turn off the AS50 power Note The REMOTE setting is not used Press Select A or Select V to select SCH schedule or DIRECT CONTROL mode When the AS50 is in LOCKED RMT mode Direct Control is always selected In Schedule mode the screen displays the number of the currently running schedule the schedule line and the method Enter the schedule number to run 1 to 9 in the SCH field Doc 031935 01 4 04 C 5 AutoSelect AS50 Autosampler C 3 Detail Status Screen Use the DETAIL ST
167. onto each vial and tighten To prevent septa from falling into the vials when they are pierced by the sampling needle make sure the cap septas are pushed fully into the caps and the caps are securely tightened NOTE If the sample temperature control option is installed Dionex recommends using vial caps with slit septa only P N 055427 package of 100 1 5 mL glass vials caps and slit septa Load the vials in the tray in the order required for the schedule if running under Local control or sequence if running under Chromeleon control See Section 3 11 for instructions on how to create a schedule of injections for Local control If the sample temperature control option is installed position tray covers P N 061010 over any empty positions on the tray see Figure 3 2 This ensures temperature stability and minimizes condensation especially at low temperature set points Tray Covers for empty positions Sample Vials Figure 3 2 Installing Tray Covers Sample Temperature Control Option Only Doc 031935 01 4 04 3 Operation and Maintenance 5 Install the tray in the autosampler compartment 6 Close the autosampler compartment door Make sure the door remains closed during operation If the door is opened during operation the sampling arm stops IMPORTANT immediately If a schedule is running it is aborted To safely open the door during operation press the Door button see Section 3 8 for
168. ontrol Range Operating Humidity Dimensions Sample Temp Autosampler Weight Sample Temp Autosampler 4 to 60 C in 1 C increments at 24 C 75 F ambient stable to 0 2 C 5 to 90 relative humidity noncondensing Cooling capacity is slightly reduced at high humidity 65 cm high x 30 cm wide x 46 cm deep 2 75 in x 12 in x 19 in 38 kg 81 Ib Doc 031935 01 4 04 AutoSelect AS50 Autosampler A 4 Doc 031935 01 4 04 B Installation This chapter provides installation instructions for the initial setup of the AutoSelect AS50 Autosampler Instructions for installing optional components such as the sample preparation option and USB connections for computer control are also provided If the installation instructions refer you to a manual for another Dionex instrument or chromatography component you can find the manual on the Dionex Reference Library CD ROM P N 053891 located in the AS50 Ship Kit P N 061270 or P N 061271 B 1 Facility Requirements Make sure the AS50 meets the power and environmental specifications listed in Appendix A Install the AS50 on a sturdy workbench at a height that ensures convenient viewing of the front panel display and access to the interior Two or more persons should lift the AS50 which weighs more than 18 kg 40 Ib Lift the AS50 only from each side of the cabinet bottom Lifting from the front door will damage the door hinges CAUTION
169. ontrol functions in external devices such as a Dionex IC system module When connected to a controlling device the inputs can be programmed to perform the following AS50 functions Start and continue a schedule e Turn the sample tray temperature control option on off NOTE The thermal compartment option is not currently available Relay outputs 1 and 2 can be programmed to switch any low voltage control Switched current must be less than 200 mA and 42 V peak D 1 1 Connecting a TTL or Relay 1 Locate the twisted pair of wires Locking Screws P N 043598 and the 12 position connector plug P N 923686 see Figure D 2 in the AS50 Ship Kit Position 1 Position 12 P N 061270 or P N 061271 Figure D 2 12 Position Connector Plug 2 Follow these basic steps to connect the TTL or relays a Foreachrelay or TTL to be used connect an active wire red and a ground wire black to the 12 position connector plug at the appropriate pin locations Refer to Figure D 1 or the label on the 850 rear panel for the connector pin assignments b To attach a wire to the plug strip the end of the wire insert it into the plug and use a screwdriver to tighten the locking screw If necessary multiple ground wires can be attached to a single TTL input output ground pin When attaching wires to the connector plug be careful not to allow stray strands of wire to short to the adjoining position on the connector CAUTI
170. ontrol panels for simultaneous injection are in the Dionex Templates Panels Dionex_IC folder 8 Create sequences programs PGMs and quantification methods QNTs for simultaneous injections See Section E 5 2 for details E 5 1 Assigning Unique Names to Devices When you add a device to a timebase the Chromeleon Server Configuration program assigns a default name to the device For example Pump for an analytical pump ECD for a conductivity detector Pump ECD for combined pump and detector and Sampler for an autosampler In addition other components for example relays TTLs and injection valves are assigned device names E 10 Doc 031935 01 4 04 E Simultaneous Injections If you set up a timebase that includes multiple devices of the same type you will need to edit the device names to ensure that each one is unique The Chromeleon Audit Trail displays error messages that identify duplicate device names in a timebase for example 11 45 59 The symbol Pump_ECD already exists NOTE Chromeleon automatically assigns unique names to the two injection valves in the AS50 To rename a device open the device Properties dialog box by double clicking the device in the timebase The General tab page displays the device name In the Device Name edit box add _2 to the existing name In the example below the device name for the second ICS 2000 in a timebase is renamed Pump ECD 2 This device will be identified
171. or port When all adjustments are complete press Home Factory calibration settings are recorded on a label on the inside of the autosampler compartment door If you changed the X Y or Z inject port coordinates during alignment record the new value s on the label If you are performing this procedure after replacing the needle seal assembly calibrate the inject port volume see Section 5 8 5 22 Doc 031935 01 4 04 5 Service 5 8 Calibrating the Inject Port Volume Calibrate the inject port volume after replacing the needle seal assembly or if reproducibility between injections is poor NOTE This procedure has numerous steps To help maintain your place in the procedure check off each step after completing it 1 Disconnect the needle seal line fitting from port 5 of the injection valve 2 Press Menu 8 and 5 to go to the LIQUID CONTROL screen 3 With the cursor in the VIAL field press Select V to select INJ and press Enter The needle arm moves to the inject port 4 Select the following options Note Throughout the procedure retain the current or default settings for options that are not listed from FLUSH SYRINGE SPEED 5 SYRINGE SAMPLE ACTION FILL 5 Press Enter Note Throughout the procedure to initiate the selected action the cursor must be in the ACTION field when you press Enter 6 Select ACTION EMPTY and press Enter 7 Select the following options ASPIRATE 60 uL from NEEDLE
172. ough the side slot on the IC system module to the injection valve Do not cut the needle seal line to shorten it and do not replace it with a different line The needle seal line is specially constructed and the autosampler will not function correctly if this line is cut Route the AS50 needle seal line to the injection valve Insert the waste line from the injection valve here Figure B 5 AS50 Drip Tray 3 Connect the needle seal line from the AS50 s C L 9 ASSOtoportS 5 on Needle the injection valve see Figure B 6 4 Locatethe green waste line connected to port W 6 on the injection valve see Figure B 6 Figure B 6 AS50 Injection Valve B 8 Doc 031935 01 4 04 B Installation This line labeled AS50 TRAY is connected with a union to a Teflon waste line see Figure B 7 which is routed through the tubing chase to the IC system module rear panel 5 Disconnect the green AS50 TRAY line from the union and route the line to the drip tray in the a autosampler AS50 TRAY compartment Waste Line Disconnect j Waste Union from the union Figure B 7 Injection Valve Waste Line Union ICS 1000 1500 2000 You can either leave the disconnected Teflon waste line and union in the tubing chase or pull the line out from the front 6 ensure the green AS50 TRAY line stays in place in the opening in the drip tray sharply bend the line about
173. ove the cursor to the ACTION field and select an action to perform For the GO TO action enter the desired xyz positions 2 Press Enter C 34 Doc 031935 01 4 04 C Display Screens C 9 4 Temperature Statistics Screen Use the TEMPERATURE STATISTICS screen to e Manually control the temperature of the optional tray compartment Monitor the status of temperature related values NOTE This screen is available only when the temperature MRE EIER control option is installed TEMPERATURE STATISTICS MIN 0 1 A 10 2 TIME 12 34 56 RAY SET 435 COL Sly WielMiiPs Sc ACTUAL 35 0 ACTUAL 10 0 A CONTROL HEX CONTRO IU Help Prompt 1125 DIU IC Cres Figure C 24 Temperature Statistics Screen Screen Field MIN MAX TIME TRAY SET TEMP COL SET TEMP ACTUAL PELTIER Description Displays the minimum and maximum values measured for the selected value and the length of time the value has been monitored An asterisk indicates the value being monitored To select a value to monitor move the cursor to the desired field and press Enter The temperature to maintain in the tray Note The COL SET TEMP fields are for support of the thermal compartment option which is not currently available Displays the measured temperature The reading is updated every 3 seconds A symbol to the right of the reading indicates the temperature s status
174. phase of the method is run Ifyou enter multiple injections per vial the sample prep phase of the method is performed once only before the first injection The method setup and timed events are performed for each injection INJ VOL Enter the volume of sample to deliver for each injection Number of Valves Syringe Size Inject Volume Ranges Default 1 1000 uL or 1 0 99 9 uL 25 uL smaller 100 1000 uL 2 simultaneous 5mL 1000 5000 uL 1000 uL injection 10 mL 1000 8000 uL The volume entered determines whether the injection is a full loop partial loop or partial loop limited sample See Section 3 11 5 for details Simultaneous injection is always full loop METHOD In the METHOD field enter the method to run 1 through 99 on each vial in the range 3 36 Doc 031935 01 4 04 3 Operation and Maintenance 3 11 5 Editing a Schedule who dE 5095 Go to the SCHEDULE screen In the EDIT field enter the number of the schedule to be edited Press Enter Change the schedule parameters Save the schedule The changes can be saved to the same method number or to a new unused method number If you save to a new number the original method remains unchanged 3 11 6 Deleting a Schedule 1 2 Go to the SCHEDULE screen Position the cursor in the EDIT field and press Delete A confirmation message appears Press Delete again to delete the schedule 3 11 7 Copying a Schedule 1 2
175. ple preparation 2 9 Sample temperature control 2 2 2 12 Organizer for reservoirs 2 2 2 9 Overlapping sample prep 2 23 P Packing instructions F 1 Panels Chromeleon Control panel 2 19 Doc 031935 01 4 04 Index 7 AutoSelect AS50 Autosampler Parameters Chromeleon operating commands 2 19 Part numbers Needle seal assembly 5 2 Syringes 5 3 Teflon washer 5 4 Tubing assemblies 5 2 USB cable B 10 USB hub B 10 Partial loop injection 3 38 Setting up 3 43 Summary of 3 38 Partial loop limited sample injection 3 44 Setting up 3 45 Summary of 3 38 Pausing a schedule 2 4 Pausing between sample prep steps 3 22 Peak Name E 14 Peak Table E 14 PEEK injection valve B 14 Periodic maintenance 3 46 PGM E 10 E 13 Pipetting between vials 2 9 3 18 3 23 C 10 Plumbing Configuration screen C 21 For simultaneous injection E 7 Selecting options B 14 Port Flush 2 11 Inject 2 11 Waste 2 11 Power Specifications A 1 Power cord B 12 Power receptacle B 12 Power Up screen 3 3 B 13 C 31 Power up sequence 3 3 B 13 Prep syringe Fluid schematic 2 32 B 19 Functions of 2 9 Specifying the volume of B 14 C 22 Preparing samples See Sample preparation Priming the liquid lines B 18 C 28 Sequence of operation 2 31 Print Menu C 44 Printer connector 2 14 Printing reports C 44 Programs Chromeleon 2 19 Putting a schedule on hold 3 10 Q QNT E 10 E 13 R Rack See Tray Rang
176. ple syringe is clipped to the upper right side of the needle arm see Figure 5 4 Using a 2 5 mm Allen wrench remove the M3 screw that attaches the clip to the sampling arm Remove the clip and then remove the tubing from the clip NOTE Some sampling arms have a clip that is open at the top This type of clip does not need to be removed To remove the tubing from the clip pull the clip out slightly and push the tubing up and out of the clip M3 Screw Tubing Clip Needle Arm Figure 5 4 Sample Transfer Line and Tubing Clip 5 8 Doc 031935 01 4 04 5 Service 10 Measure the distance from the yellow sleeve on the sample transfer line see Figure 5 4 to the needle When installing the new sample transfer line position the sleeve on the new line the same distance from the needle The sample transfer line is coiled and held by a bracket and clamp on the upper right side of the autosampler compartment see Figure 5 5 To remove the tubing use a flathead screwdriver to gently pry the center bracket apart Then lift the tubing out of the bracket and clamp Measure the distance from the second yellow sleeve see Figure 5 5 to the needle When installing the new sample transfer line position the sleeve on the new line the same distance from the needle Disconnect the sample transfer line from port C on the sample syringe valve Pull the ferrule f
177. pped with two injection valves for simultaneous injections these fields display and let you set the position of each valve Injection valve 1 is the bottom valve and injection valve 2 is the top valve See Appendix E for details about the simultaneous injection option Values A column A B column B L load I inject Doc 031935 01 4 04 C Display Screens C 4 Method Menu Use the METHOD MENU to Enter the number of the method to be created or edited Enter the number to save the method to Access the three method editing screens METHOD MENU 2 5 SAVE 25 1 S AMIBIISESPIRIEIP 2 ES ETUR 3 Help Prompt Figure C 5 Method Menu To open a method editing screen move the cursor to the screen s number and press Enter To return to this screen press Menu NOTE A method can have a maximum of 100 steps The total includes the sample prep steps the method setup which counts as one step and the timed events steps For example a default method would have a total of four steps one for the sample prep Wait step one for the method setup and two timed events INIT and time 0 00 Doc 031935 01 4 04 C 9 AutoSelect AS50 Autosampler 4 1 Sample Prep Screen Use the SAMPLE PREP screen to specify a sequence of operations to be performed before the method s timed events NOTE See Section 3 10 3 for details about how to define sample prep steps SAM
178. procedures for how to isolate and eliminate the cause of each problem Provides step by step instructions for routine service and parts replacement procedures that the user can perform Lists the 550 specifications and installation site requirements Describes how to install the AS50 Describes the front panel operational and diagnostic display screens Describes the 550 TTL and relay control features Describes the features of the simultaneous injection option and provides setup and operating instructions Provides step by step instructions for removing the AS50 from its shipping box and packing materials For instructions on using Chromeleon software to operate the AS50 refer to the Chromeleon user s guide or online Help The user s guide is on the Dionex Reference Library CD ROM P N 053891 located in the AS50 Ship Kit P N 061270 or P N 061271 Doc 031935 01 4 04 1 Introduction 1 2 1 gt DANGER gt WARNING gt CAUTION IMPORTANT gt DANGER gt AVERTISSEMENT gt MISE EN GARDE Safety Messages and Notes This manual contains warnings and precautionary statements that can prevent personal injury and or damage to the AS50 when properly followed Safety messages appear in bold type and are accompanied by icons as shown below Indicates an imminently hazardous situation which if not avoided will result in death or serious injury Indicates a potentially
179. ption not currently available All off 0 Stop 1 1 1 25 0 uL 1 mL for simultaneous injection 1 Enabled 3 10 uL varies each inject port is individually calibrated 8 uL 60 uL for simultaneous injection cannot be changed by user 250 uL Flush always Sample always 400 uL Flush Sample Doc 031935 01 4 04 3 Operation and Maintenance 3 4 Selecting Computer or Front Panel Local Control When the AS50 is powered up it is always in front panel Local control To select computer control start Chromeleon and open the Control panel for the timebase to which the AS50 is assigned Connect the AS50 timebase to the Control panel When the AS50 is under computer control the front panel MAIN and DETAIL screens indicate this by displaying LOCKED RMT and DIRECT CONTROL in the operating and control mode fields See the example in Figure 3 4 IDLE VIAL S TIME 99 9 min INJ 1 1 VOL 25 0u TRAYS e 9 COL METHOD 0 LOCKED RMT DIRECT CONTROL Help Prompt Operating Mode Field Control Mode Field Figure 3 4 Main Screen Computer Control To return the AS50 to Local mode disconnect the AS50 from the Chromeleon Control panel clear the Connected check box The operating mode field on the MAIN and DETAIL screens now displays LOCAL See the example in Figure 3 5 IDLE VIAL 5 TIME 99 9 min INJ 1 1 VOL 259 TRAY s COL C METHOD 0 LOCAL DIRECT CONTROL Help Prompt Figure
180. quipped with the simultaneous this line injection option disconnect the needle seal a e line from the Y connector e E in the autosampler 1 compartment see Figure 5 14 IN Figure 5 14 Simultaneous Injection Option Disconnecting the Needle Seal Line Disconnect 3 Rotate the fitting below the inject port until the slots in the needle seal align with the projections on the top of the inject port see Figure 5 15 Needle Seal and Inject Port see top view below Rotate this fitting Align slots with inject port projections Top View Needle Seal afa Figure 5 15 Hemoving the Needle Seal Assembly Doc 031935 01 4 04 5 17 AutoSelect AS50 Autosampler 4 Push up from underneath and slide the entire needle seal assembly up and out of the inject port 5 Remove the ferrule and bolt fitting from the end of the needle seal line Save the fitting Do not cut the needle seal line to shorten it and do not replace it with IMPORTANT a different line The needle seal line is specially constructed and the autosampler will not function correctly if this line is cut 6 Thread the new needle seal line see Figure 5 16 down through the top of the inject port prs P Needle f Seal Line 18 in Ns PEEK version Needle j i shown Seal Line A Needle Seal Figure 5 16 Needle Seal Assembly 7 Align the slots on the needle seal with the projections o
181. r Notes The TRAY field is available only when the sample temperature control option is installed Tray temperature can be set to between 4 C and 60 C Dionex does not recommend using the 10 mL plastic tray with the sample temperature control option because the plastic tray is inefficient at heating and cooling the vials In addition the tray is not designed for temperatures above 40 C 104 F and may become deformed over time if used above 40 C The CS VLV field is used to switch the position of a column switching valve The column switching valve option is not currently available for the AS50 USB Keep the default setting A for this field e If the simultaneous injection option is installed the CS VLV field is replaced with a field for controlling the second injection valve See Appendix E for details about simultaneous injections Doc 031935 01 4 04 3 15 AutoSelect AS50 Autosampler 3 10 Creating Methods from the AS50 Front Panel NOTE This section describes how to create methods from the front panel Local mode To create methods from the computer refer to the Chromeleon user s guide or online Help A method defines a series of operating instructions that tells the AS50 how to perform a single injection A method consists of three phases e Sample prep Section 3 10 3 Method setup Section 3 10 4 e Timed events Section 3 10 5 Parameters for these are defined on separate screens A meth
182. ration could not be completed because vial tray is not installed 4 6 Received invalid command from DSP 4 8 Recoverable motion error occurred in X axis 4 9 Response from syringe was too long 4 5 Sampler door opened 4 13 Schedule error Specified schedule does not exist 4 8 Specified volume is larger than size of sample syringe 4 10 Syringe reports error 4 18 Syringe reports fatal error 4 18 Timed events step took so long to execute that at least one subsequent step was missed 4 11 Timed out waiting for home position to be found 4 12 Timed out waiting for motion complete command from DSP 4 8 Timed out waiting for tray temperature to stabilize 4 20 Timeout occurred waiting for syringe serial port txrdy 4 4 Timeout occurred waiting for syringe to complete an action 4 4 Timeout occurred waiting for syringe to respond to command 4 5 Error sound Turning on and off C 20 Example method 3 32 Extractions 2 10 F Facility requirements B 1 Factory set default 3 6 Filling a new syringe 5 4 Fittings Leaking 4 22 Replacing 5 2 Flush fluid B 4 Flush port 2 11 Flush Prime screen C 28 Flushing the inject port 2 30 3 48 C 28 Sequence of operations 2 30 Flushing the needle 3 18 3 24 C 11 Flushing the sample loop 2 30 Format of calibration database has changed 4 12 Format of global database has changed 4 11 Format of method schedule database has changed 4 12 Front panel 2 2 2 3
183. ratures above 40 C 104 F and may become deformed over time if used above 40 C 2 3 5 Sample Temperature Control Option The optional sample temperature control unit provides heating and cooling of the sample vial tray You can program the temperature set point to between 4 C and 60 C Vials in the tray are uniformly heated or cooled to the programmed set point to a minimum of 20 C below ambient and a maximum of 40 C above ambient The sample temperature control unit sits on the workbench below the autosampler compartment see Figure 2 7 Sample Temperature Control Option Figure 2 7 AS50 with Sample Temperature Control 2 12 Doc 031935 01 4 04 2 Description 2 3 6 Simultaneous Injection Option The AS50 simultaneous injection feature lets a single AS50 deliver sample to two independent IC ion chromatography systems The two IC systems are plumbed in parallel and connected to two injection valves installed either in the ASSO autosampler compartment or in the IC system modules Dual analyses can be performed with only one sample thus increasing sample throughput and eliminating the need to label fill and track two sample vials The simultaneous injection option requires a 5 mL or 10 mL sample syringe Full loop injections are required with this option See Appendix E for detailed simultaneous injection setup and operation instructions Doc 031935 01 4 04 2 13 AutoSe
184. re the white Teflon washer remains installed inside the port Remove the acorn nut from the line that extends from the flush reservoir cap and connect the line to port E see Figure B 1 Doc 031935 01 4 04 B Installation 7 Ifthe sample temperature control option is installed locate the drain line P N 055075 in the AS50 Ship Kit P N 061271 and push the line onto the fitting on the lower right front corner of the sampler temperature control unit 8 Place the end of the drain line that extends from the lower right front corner of the autosampler compartment see Figure B 2 or sample temperature control unit into a waste container Drain Line Figure B 2 Autosampler Compartment Drain Line For correct drainage make sure the tubing is not bent pinched or IMPORTANT elevated at any point Do not allow the end of the waste line to be submerged in waste liquid Neutralize acidic and caustic wastes before disposal Dispose of all wastes in accordance with local regulations CAUTION Doc 031935 01 4 04 B 5 AutoSelect AS50 Autosampler Neutralisez les d chets acides ou caustiques avant de les jeter Jetez les d chets aux r glements locaux MISE EN GARDE Neutralisieren Sie saurehaltige und atzende Abfalle vor ihrer Entsorgung Entsorgen Sie alle Abfalle entsprechend den lokalen Bestimmungen VORSICHT 9 Connect the cable from the sample syringe to the SAMPLE SYRINGE connector on the re
185. re you want the new step to appear and press Insert Notes e The new step is inserted above the cursor The START and END vial numbers are set to one more than the previous end number Other parameters injections per vial injection volume and method number are copied from the previous step e If you insert a new step at the beginning of a schedule parameters are copied from the first step To add a step at the end of a schedule position the cursor on the blank step at the bottom of the screen and press Insert Deleting Steps Position the cursor in the step s LINE field and press Delete twice Doc 031935 01 4 04 3 35 AutoSelect AS50 Autosampler LINE After you enter the START and END vial numbers the LINE field displays the appropriate schedule line numbers You cannot edit the LINE field START and END Enter the starting and ending vials for a range of vials to be sampled Tray Type Tray Capacity Valid Vial Numbers 1 5 mL aluminum 100 vials 1 5 or 0 3 mL 1 100 10 mL plastic 49 PolyVials 1 49 e A range is a group of consecutively numbered vials that will be sampled using the same schedule parameter values injections per vial injection volume and method number The starting vial can be a higher number than the ending vial e Fora single vial enter the same number for START and END INJ VIAL Enter the number of injections 0 to 99 to perform on each vial Ifyou enter 0 only the sample prep
186. refilling the flush reservoir manually flush the inject port see Section 3 14 4 Check the volume of liquid in the waste container and empty when needed Neutralize acidic and caustic wastes before disposal Dispose of all wastes in accordance with local regulations Neutralisez les d chets acides ou caustiques avant de les jeter Jetez les d chets aux r glements locaux Neutralisieren Sie s urehaltige und atzende Abf lle vor ihrer Entsorgung Entsorgen Sie alle Abfalle entsprechend den lokalen Bestimmungen For correct drainage make sure the end of the waste line is not submerged in waste liquid Periodically Check the alignment of the sampling arm needle If the needle is not centered in the inject port opening when it enters the port realign the needle See Section 5 7 for instructions Remove the drip tray see Section 5 4 rinse the tray and the leak sensor dry thoroughly and replace see Section 5 5 Doc 031935 01 4 04 3 Operation and Maintenance Check for leaks from the inject port the syringe s the syringe valve s and the sampling needle line See Section 4 2 if leaks occur 3 14 3 Annually Perform the AS50 preventive maintenance procedure An AS50 Preventive Maintenance Kit is available for this purpose The kit provides parts and instructions for performing the procedure Doc 031935 01 4 04 3 47 AutoSelect AS50 Autosampler 3 14 4 Manually Flushing the Inject Port Flu
187. remove unused channels from Online Change Apply Help 12 Doc 031935 01 4 04 E Simultaneous Injections Also select the State Devices tab and add _2 to the name of each enabled device Press F2 to edit a device name See the following example Dionex ICS 2000 System Demo Chromatogram TTL Inputs Trend Calibration Diagnostics General Options Signals Head Type amp Limits Solvents Eluent Generator State Devices Emorlevels Name Pump ECD Relay 1 2 Pump ECD Relay 2 2 Pump ECD TTL 1 2 Pump ECD TTL 2 2 Pump InjeciValve 2 Pump ECD Analog Out 2 Deactivate check boxes to remove unused relays from Online Hit F2 to edit selection Cancel Apply Help E 5 2 Creating Sequences PGM Files QNT Files Follow these guidelines when creating sequences PGM files and QNT files for simultaneous injection Create one sequence that includes calibration standards for both systems and the unknown samples e Create a file for the unknowns that includes commands for controlling both systems Calibrate each system separately by creating separate calibration PGM files Createasingle QNT file that includes the components from both systems Doc 031935 01 4 04 E 13 AutoSelect AS50 Autosampler Creating a Method File for Systems Sharing an AS50 For simultaneous injection analyses create a single method file wit
188. required see the previous priming guidelines 8 When priming is complete there are often small air bubbles in the top area of the syringe Remove the bubbles before proceeding See Section B 3 11 for instructions B 20 Doc 031935 01 4 04 B Installation B 3 11 Removing Bubbles from the Syringe 10 Fill a container with isopropyl alcohol Using a flathead screwdriver unscrew and remove the syringe drive connecting screw see Figure B 16 Hold the syringe at the top and bottom and unscrew it from the valve Point the threaded end up into the air Push the syringe plunger up and pull down rapidly with short movements to dislodge the bubbles Repeat until all bubbles are dislodged Place the threaded end of the syringe into the isopropyl alcohol and slowly pull out the plunger to draw a full syringe volume into the syringe Syringe Drive Connecting Screw Remove Figure B 16 Removing the Syringe Keeping the syringe in the liquid slowly push the syringe plunger until the bubble s exits the syringe Then fill the syringe Remove the syringe from the liquid While holding it vertically push a small amount of liquid out the top to make sure there is no air present Position the syringe under the valve Holding the syringe at the top and bottom screw it back into the valve and tighten fingertight Align the syringe piston with the connecti
189. ress the Insert button e To insert a step below the wait line position the cursor on the blank line below wait and press the Insert button Enabling and Disabling the Wait Operation 1 If you are editing a method save the current settings see Section 3 10 2 2 Go to the MODULE SETUP menu and press 5 to go to the SYSTEM PARAMETERS screen 3 Position the cursor in the WAIT field and press Select A or Select V to toggle the field to ENABLED or DISABLED 4 Press Enter Doc 031935 01 4 04 3 21 AutoSelect AS50 Autosampler Specifying a Delay Between Prep Steps The DELAY operation specifies the number of minutes 550 waits before proceeding to the next step in the sample prep phase DELAY 0 0 min Number of Minutes to Delay Before Performing Next Step Delay Time 0 0 to 999 9 min Specifying a Needle Height The NEEDLE operation positions the tip of the needle at the distance above the bottom of the vial Zero is closest to the bottom 46 is closest to the top of the vial The height applies to subsequent PIPET MIX and DISPENSE operations When pipetting the height applies only to the source vial NOTE For 0 3 mL and 1 5 mL vials use a maximum needle height of 2 mm to ensure the needle s vent is positioned correctly inside the vial NEEDLE 2 mm Height of Needle from Bottom of Vial Needle Height 0 to 46 mm NOTE The needle height specified here does not change the d
190. rned off Press Select A or Select V to toggle tray temperature control to on Press numeric buttons to enter the desired temperature setting 4 Press Enter Doc 031935 01 4 04 3 27 AutoSelect AS50 Autosampler Waiting for the Temperature to Stabilize The WAIT FOR TEMP STABLE parameter delays the start of the method until the temperature of the sample tray has reached the specified set point s 1 Position the cursor in the WAIT FOR TEMP STABLE field 2 Press Select A or Select V to select either YES wait or NO do not wait 3 Press Enter 3 10 5 Defining Timed Events Steps To go to the TIMED EVENTS screen 1 Go to the METHOD MENU 2 Position the cursor in the 3 field TIMED EVENTS 3 Press Enter Figure 3 16 shows the default screen TIMED EVENTS METHOD 25 TIME VALVE CSV TTLI TTE2 RLY1 RLY2 INIT LOAD A 0 0 0 0 0 00 IN ECT Help Prompt Figure 3 16 Timed Events Screen Default NOTE If the simultaneous injection option is installed the CS VLV field is replaced with a field for controlling the second injection valve See Appendix E for details about simultaneous injections 3 28 Doc 031935 01 4 04 3 Operation and Maintenance Understanding the INIT and Time Zero Steps The method timed events always start with two steps The parameters in these steps can be changed but the steps cannot be deleted e first step is the INIT initial conditions step The initia
191. s and or executes the change Displays a help screen specific to the current entry field Displays a list of the available screens The numeric buttons enter the selected number into the current entry field From a menu pressing a numeric button opens the corresponding screen Saves and or executes changes made in entry fields If a menu screen is displayed pressing Enter opens the highlighted screen Doc 031935 01 4 04 2 5 AutoSelect AS50 Autosampler 2 2 2 Display Screens The LCD displays status information and allows access to all AS50 operations When the power is turned on the 550 performs an initialization sequence see Section 3 1 2 and then displays the MAIN STATUS screen Figure 2 3 is an example of the MAIN STATUS screen during AS50 operation Current Vial Operating Status LOADING LOOP un Current Injection ast Injection Ts Injections VIAL l 0 1 Volume to be cheduled for Injected This Vial 1 2 3 Y 20 C COL C 3 Current Method Operatin Mode Schedule Line Current Tray Control Current Currently in Temperature Mode Schedule Progress Figure 2 3 Main Status Screen NOTE TRAY temperature is displayed only when the sample temperature control option is installed The column COL temperature option is not currently used 2 6 Doc 031935 01 4 04 2 Description To access other AS50 screens Press the Menu button The MENU of SCREENS appears see
192. s position in the sample prep steps determines whether samples are run overlapped or sequentially When the wait is the last step in the sample prep phase the default samples are overlapped see Section 2 6 2 e When the wait is at the beginning of the sample prep phase samples are run sequentially see Section 2 6 3 NOTE The wait operation can be disabled see Section 2 6 4 2 22 Doc 031935 01 4 04 2 Description 2 6 2 Overlapping Sample Preparation When the wait is at the end of the sample prep phase the default the AS50 overlaps sample preparation This means that while data collection is occurring for one injection the AS50 performs the sample prep steps for the next injection see Figure 2 13 For a detailed description of the schedule run flow of operations see Section 2 6 6 FLUSH NOTE Start First Method Injection 1 WASH SAMPLE PREP SETUP INIT TIMED EVENTS The WAIT is ignored for Start Next Method Injection 2 the first injection in a pt Schedule WASH SAMPLE PREP WAIT SETUP INIT TIMED EVENTS L Overlap FLUSH i ee Overlap Wait for Continue CONTINUE Start Next Methpd Command WASH SAMPLE PREP WAIT FLUSH Figure 2 13 Sample Prep Overlap Wait Operation at End of Sample Prep Phase Default NOTE If Chromeleon is controlling the AS50 front panel settings for the wait operation are ignored Instead an opt
193. setup and configuration information or a message log In addition automatic schedule printouts can be enabled or disabled from here The communication parameters for setting up the serial printer are listed on the next page PRINT MENU PRINT SCHEDULE PRINT METHOD PRINT SETUP CONEIG PRINT MESSAGE LOG AUTOMATIC PRINTING DISABLED elp Prompt Screen Field PRINT SCHEDULE PRINT METHOD PRINT SETUP CONFIG PRINT MESSAGE LOG AUTOMATIC PRINTING Figure C 33 Print Menu Description Displays a prompt screen on which you enter the schedule number to be printed Displays a prompt screen on which you enter the method number to be printed Prints setup and configuration information Prints the message log up to 20 messages Toggles between DISABLED and ENABLED When enabled schedule information will print automatically while the schedule is running C 44 Doc 031935 01 4 04 C Display Screens RS 232 Interface Communication Parameters Parameter Baud Mode Data Bits Parity Stop Bits Flow Control Connector Specification 9600 Full duplex 8 None 1 XON XOFF DB9 male jack Doc 031935 01 4 04 C 45 AutoSelect AS50 Autosampler C 11 Time Function Out Screen Use the TIME FUNCTION OUT screen to enable TTL control of an injection valve installed in an ICS 1000 ICS 1500 or ICS 2000 ENABLED DISABLED Help Prompt Figure C 34 Time Function Out Screen
194. sh the inject port after refilling the flush reservoir The AS50 must be idle to initiate a flush cycle 1 Set the 550 to LOCAL mode and DIRECT CONTROL 2 Goto the FLUSH PRIME screen press Menu and 6 and enter the desired FLUSH VOLUME or keep the default value 3 Move the cursor to the ACTION field and select FLUSH see Figure 3 25 4 Press Enter to start the flush FLUSH PRIME FLUSH PRIME VOLUME 400 uL VOLUME 400 uL RESERVOIR RESA SYRINGE SAMPLE SYRINGE PREP ACTION FLUSH ACTION READY Help Prompt Figure 3 25 Initiating a Manual Flush Cycle 3 15 System Shutdown No special system shutdown procedure is required To automatically shut down if you are using Chromeleon to control the 550 load a shutdown program at the end of the schedule that turns off the pump flow and the suppressor current 3 48 Doc 031935 01 4 04 4 1 4 Troubleshooting This chapter is a guide to troubleshooting minor problems that may occur while operating the AutoSelect AS50 Autosampler e Section 4 1 describes error messages and how to troubleshoot them e The remaining sections in this chapter describe other operating problems Turn to the section that best describes the operating problem There possible causes of the problem are listed in order of probability For additional help refer to Appendix C for instructions on running the AS50 diagnostics program If you are unable to eliminat
195. sible Causes An error occurred during X axis movement of the sampling arm The DSP was not able to recover from the error Actions 1 Turn off the power 2 Check for an obstruction in the autosampler compartment 3 Check for freedom of movement in all three axes of the sampling arm 4 Turn on the power again If the problem persists contact Dionex Doc 031935 01 4 04 4 9 AutoSelect AS50 Autosampler 414 415 Specified volume is larger than size of sample syringe During normal operation not during diagnostics the syringe can perform up to four multiple strokes to aspirate or dispense a volume up to four times larger than the syringe size Possible Causes During a schedule or flush prime operation the specified volume is more than four times the size of the sample syringe Onthe LIQUID CONTROL diagnostics screen either the specified volume exceeds the size of the sample syringe or the cumulative volume when performing multiple actions exceeds the size of the syringe Actions Reduce the volume specified or if the sample preparation option is installed use the prep syringe not the sample syringe for certain operations See Sample Preparation Option Functions on page 2 9 for details Onthe LIQUID CONTROL screen select a different operation or reduce the volume specified Cycle time expired before INIT step was completed When the cycle time expires the AS50 ignores the cy
196. slowest to 5 fastest default 4 INJECT LOAD A column A default B column B INJECT LOAD C 38 Doc 031935 01 4 04 C Display Screens C 9 6 Logs Menu Figure C 27 Logs Menu Screen Usage Log Screen Use the USAGE LOG screen to Display for how long various AS50 components have been in use Reset counters after replacing a component The status of each parameter is updated in real time Figure C 28 Usage Log Screen MODULE ON The number of hours the AS50 has been powered up BACKLIGHT The number of hours the backlight has been on NUMBER OF INJ The total number of injections Reset the count after replacing the injection valve SAMP SYRINGE The total number of aspirate dispense cycles the sample syringe USE has performed Reset the count after replacing the syringe Doc 031935 01 4 04 C 39 AutoSelect AS50 Autosampler Screen Field Description PREP SYRINGE The total number of aspirate dispense cycles the prep syringe USE has performed Reset the count after replacing the syringe RESET To reset the NUMBER OF INJ SAMP SYRINGE USE or PREP SYRINGE USE counter to 0 move the cursor to the corresponding asterisk field and press Enter Message Log Screen Displays a list of errors that have occurred MESSAGE LOG 112233 29 SEPTEMBER 04 Incorrect key pressed 12 13 14 29 SEPTEMBER 04 Missing vial detected 1567 02 29 SEPTEMBER 04 Method does not exist Help Promp
197. ss Enter Factory calibration settings are recorded on a label on the inside of the autosampler compartment door Record the new inject port volume on the label If you are performing this procedure after replacing the needle seal assembly a Slide the bolt and ferrule removed from the old needle seal line onto the end of the new needle seal line or install a new bolt and ferrule fitting b Connect the needle seal line fitting to the injection valve Tighten the fitting fingertight and then tighten it an additional one quarter turn using the 5 16 in wrench 5 26 Doc 031935 01 4 04 5 Service 5 9 5 10 Replacing the Leak Sensor Remove the drip tray from the autosampler see Section 5 4 Unhook the leak sensor from the drip tray and pull the leak sensor cable out until the connector is visible Unplug the old leak sensor and connect a new leak sensor P N 053669 Attach the sensor to the drip tray push the cable inside the autosampler compartment and reinstall the drip tray see Section 5 5 NOTE Make sure the bottom wire on the leak sensor is not touching the tray There should be at least a paper width gap between it and the tray NOTE After installing the drip tray make sure the tray is not tilted to the left if it is liquid will not drain properly during operation 5 Calibrate the new sensor see Section 5 10 Calibrating the Leak Sensor NOTE You can also calibrate the leak sensor from
198. t Figure C 29 Message Log Screen Each message includes the time and date the message was recorded the error code and a brief description of the error To see a full screen description of an error move the cursor to the error s entry number and press Help Press Select A or Select V to go to the beginning or ending of the log respectively C 40 Doc 031935 01 4 04 C Display Screens C 9 7 Leak Sensor Calibration and Status Screen Use this screen to display the status of the leak sensors and to calibrate them LEAK SENSOR CALIBRATION AND STATUS TRAY OVEN MEASURED VALUE 2 48 P CURRENT CONDITION DRY CALIBRATION VALUE 2 49 LOW LEAK THRESHOLD 2 29 ENABLE LEAK DETECTION YES Help Prompt Figure C 30 Leak Sensor Calibration and Status Screen Screen Field MEASURED VALUE CURRENT CONDITION CALIBRATION VALUE LOW LEAK THRESHOLD Description Reports the current measured voltages for the two leak sensors TRAY is the sensor installed in the autosampler drip tray The OVEN fields are for support of the thermal compartment option Note The thermal compartment option is not currently available Reports the current state of the sensors DRY if the sensor reading is within the dry range WET if the sensor reading is within the wet range or ERR if the sensor is out of range If the measured value drops 0 02 volts below the calibration value the current condition is wet If the measured val
199. t able to reach the temperature set point within the time allotted Possible Causes Ambient temperature too high or low e Airflow to cool the Peltier devices is restricted Malfunction of the sample temperature control option Actions e Go to the TEMPERATURE STATISTICS screen press Menu 8 and 4 and check the following parameters under TRAY SET TEMP PELTIER The Peltier value should be above zero If it is not contact Dionex DUTY CYCLE A value of 255 means the sample temperature control unit is working to capacity The ambient temperature may be too high or too low or the airflow to the Peltier device may be restricted Select a temperature that is closer to ambient If possible adjust the ambient temperature before running the 550 Check the fan intake under the ASSO and the fan exhaust in the back for any obstructions restricting airflow Make sure there is at least 6 cm 2 5 in free space behind the AS50 for ventilation Ifthe problem persists contact Dionex 4 20 Doc 031935 01 4 04 4 Troubleshooting 4 2 Liquid Leaks 4 2 1 Leaking Syringe or Syringe Valve Port Possible Causes Cracked syringe Missing or worn Teflon amp washer in the leaking valve port Loose syringe Actions e Check fora crack in the syringe Replace the syringe if necessary see Section 5 11 e Ifthe leak is from one of the valve ports remove the valve and syringe see Section 5 11 Remove the ex
200. t of the autosampler The leak sensor is hooked onto the tray and its cable is connected inside the autosampler To remove the drip tray completely from the autosampler unhook the leak sensor from the tray Injection Valve Waste Line Drip Tray Needle Seal Line Figure 5 10 Removing the Drip Tray 5 14 Doc 031935 01 4 04 5 Service 5 5 Installing the Drip Tray NOTE This section is provided as a reference when performing service procedures that require removing and reinstalling the drip tray 1 Hook the leak sensor on the tray as shown in Figure 5 11 make sure it is pushed all the way down onto the tray edge 7 ERE l Leak Sensor LS I Figure 5 11 Drip Tray with Leak Sensor Installed NOTE Make sure the bottom wire on the leak sensor is not touching the tray There should be at least a paper width gap between it and the tray 2 Tilt the top of the tray in and the bottom out and partially slide the tray back into the autosampler make sure the leak sensor clears the front overhang on the autosampler 3 Make sure the needle seal line is in the front of the tray see Figure 5 10 4 Straighten the tray and push it back into the autosampler until it is flush with the front Replace the screw NOTE After installing the drip tray make sure the tray is not tilted to the left if it is liquid will not drain properly during operation Doc 031935 0
201. t port see Section 5 7 Leaking Fitting Locate the source of the leak Tighten or replace the liquid line connection see Section 5 1 Refer to Installation of Dionex Ferrule Fittings for tightening requirements Broken Liquid Line Replace the line and fittings see Section 5 1 4 22 Doc 031935 01 4 04 5 Service This chapter describes AS50 service and repair procedures that the user can perform All procedures not included here including electronics related repair procedures must be performed by Dionex personnel For assistance contact Dionex Technical Support In the U S call 1 800 346 6390 Outside the U S call the nearest Dionex office Before replacing any parts refer to the troubleshooting information in Chapter 4 to isolate the cause of the problem a S CR The CPU card contains a lithium battery If the CPU card is replaced dispose of the used battery according to the manufacturer s instructions ens IMPORTANT Substituting non Dionex parts may impair AS50 performance thereby voiding the product warranty Refer to the warranty statement in the Dionex Terms and Conditions for more information Doc 031935 01 4 04 5 1 AutoSelect AS50 Autosampler 5 1 Replacing Tubing and Fittings The following tables list the tubing fittings and tubing assemblies used to plumb the AS50 autosampler compartment 5 1 1 Part Number 052112 048949 052267 5 1 2 Assem
202. tes provides an adequate wait period between each injection for opening the door If a method has a long sample prep phase however a longer cycle time may be required e Ifthe 550 is operating under Chromeleon control the door can be opened only when the sampling arm is at the home position and the status displays WAITING This occurs during the wait step of the sample prep portion of a method e Ifthe 550 never reaches a status that allows opening the door check for one of the following e Ifthe AS50 is operating under front panel control verify that a cycle time was specified for each method in the schedule and that the cycle time is long enough to provide a wait period between injections If the AS50 is operating under Chromeleon control check the run length specified for the Chromeleon Method It may need to be increased to allow a longer wait period between injections To cancel the OPEN DOOR SAFETY screen press any front panel button If the door is open a message cautions you that pressing a button again will abort the schedule Doc 031935 01 4 04 3 13 AutoSelect AS50 Autosampler 3 9 Running Under Direct Control from the Front Panel NOTE This section describes how to control the AS50 directly from the front panel Local mode To run under direct control from the computer Locked Remote mode refer to the Chromeleon user s guide or online Help The 550 must be in Direct Control to initiate a man
203. to 4 Deliver sample cushion the sample to the loop KEY Deionized Water mm Air mm Eluent Sample Figure 3 21 Full Loop Injection Sequence The maximum loop size for full loop injections is 300 uL 1100 uL for simultaneous injections If a larger loop size is used sample can contaminate the flush bottle because the sample volume drawn exceeds the sample transfer line volume of 1200 uL 8200 uL for simultaneous injections 3 40 Doc 031935 01 4 04 3 Operation and Maintenance Setting Up a Full Loop Injection When defining injection parameters on the SCHEDULE screen see Figure 3 22 enter an injection volume equal to or greater than the volume of the loop installed on the injection valve NOTE Entering an injection volume greater than the sample loop volume sets up the 550 for a full loop injection The actual volume of sample injected will equal the sample loop volume NOTE The loop volume is entered on the PLUMBING CONFIGURATION screen see Section C 6 3 SCHEDULE EDIT SAVE TO RUN MISSING VIAL ACTION STOP VIAL INJ INJ LINE START END VIAL VOL uL METHOD 1 181i 1 25 0 1 Help Prompt Enter the injection volume Figure 3 22 Schedule Screen Doc 031935 01 4 04 3 41 AutoSelect AS50 Autosampler 3 12 2 Partial Loop Injections When performing a partial loop injection the AS50 draws the volume to be injected from the sample vial plus two times the cut segment volume The
204. to go to the DOOR INTERLOCK BYPASS screen DOOR INTERLOCK BYPASS DOOR INTERLOCK BYPASS YES NOTE Afer setting bypass you have a 15 minute window to perform operations with the sample door open Help Prompt Figure 5 18 Door Interlock Bypass Screen Doc 031935 01 4 04 5 19 AutoSelect AS50 Autosampler b Press Select A or Select V to select YES bypass the door lock and press Enter The door lock will be disabled for 15 minutes You can now perform the alignment with the door open To extend the bypass for another 15 minutes select YES and press Enter again This restarts the 15 minute window Be aware of unexpected sampling arm movement when operating with the door open CAUTION Soyez conscient de la possibilit de mouvements impr vus du bras d chantillon lorsqu il est utilis avec la porte ouverte MISE EN GARDE Achten Sie beim Betrieb mit gedffneter T r auf unerwartete Bewegungen des Probenarms VORSICHT 3 Press Menu and 6 to go to the INJECT PORT ALIGNMENT screen INJ ECT PORT ALIGNMENT X ADDRESS 1340 Y ADDRESS 1930 Z ADDRESS 230 Help Prompt Figure 5 19 Inject Port Alignment Screen 4 Record the current values in the X Y and Z ADDRESS fields X Y Z 5 Verify that the cursor is in the X ADDRESS field and press Enter The needle arm moves to the inject port 6 Move the cursor to the Z ADDRESS field and enter 180 Press Enter The needle moves down 180 steps Note Each step is 0
205. ttings 5 2 5 1 1 Syringe and Reservoir Connections 5 2 5 1 2 Assemblies Tubing and Fittings 5 2 iv Doc 031935 01 4 04 Contents 5 2 5 3 5 4 5 5 5 6 5 7 5 8 5 9 5 10 5 11 5 12 5 13 5 14 5 15 Replacing the Sample or Prep Syringe 5 3 5 2 1 Removing The Existing Syringe 5 4 5 2 2 Filling the New Syringe and Removing Bubbles 5 4 5 2 3 Connecting the New Syringe and Flushing 5 5 5 24 Initializing the Syringe 5 5 5 2 5 Setting the Syringe Home Position 5 7 Replacing the Sampling Needle Assembly 5 8 5 3 1 Removing the Old Sampling Needle Assembly 5 8 5 3 2 Installing the New Sampling Needle Assembly 5 11 Removing the Drip Tray 5 14 Installing the Drip 5 15 Replacing the Needle Seal Assembly 5 17 Aligning the Sampling Needle in the Inject Port 5 19 Calibrating the Inject Port Volume 5 23 Replacing the Leak Sensor 5 27 Calibrating the Leak Sensor 5 27 Removing the Sample or Prep Syringe Valve 5 28 Replacing the Sample Syringe Valve
206. ual flush or priming cycle or to perform maintenance or diagnostic procedures The following AS50 functions can also be controlled directly not as part of a schedule TTL and relay outputs Injection valve position Injection valve 2 position if installed Tray temperature set point if installed Selecting Direct Control Parameters 1 Goto the DETAIL STATUS screen press Menu and 2 2 Ifthe AS50 is not in Direct Control mode position the cursor in the control mode field and press Select A or Select V to toggle to DIRECT CONTROL Press Enter DETAIL IDLE VIAL 4 MUME i INJ VOL TRAY RLY 1 TRAY 3 25 20 867 100 iE RLY 2 LLL INJ VLV LOCAL DIRECT CONTROL CS VLV Help Prompt Figure 3 11 Detail Status Screen Example 3 Position the cursor in the field for the parameter to be changed 3 14 Doc 031935 01 4 04 3 Operation and Maintenance 4 To set the TRAY temperature 0 a Ifthe field is displaying temperature control is currently turned off Press Select A or Select V to turn on temperature control b Press numeric buttons to enter the desired temperature setting 5 For other parameters press Select A or Select V to select the desired setting TTL and relay fields Select 1 for on or 0 for off e Inject valve s Select L to set the valve to load or I to set the valve to inject 6 After setting a parameter press Ente
207. ue goes 0 06 volts above the calibration value the sensor needs to be recalibrated To calibrate a sensor press Select A or Select V to select CAL and then press Enter The current measured value becomes the new calibration value See Section 5 10 for detailed instructions Reports the voltage values set for the calibration Reports the minimum voltage reading that will be interpreted as a dry sensor A reading below this voltage indicates a wet sensor Doc 031935 01 4 04 C 41 AutoSelect AS50 Autosampler C 9 8 Keyboard Test Screen Use the KEYBOARD TEST screen to conduct an interactive test of the front panel keypad buttons Figure C 31 Keyboard Test Screen To test the buttons 1 Press a button on the keypad Its display changes to reverse video confirming proper operation of that button 2 Continue pressing all buttons in turn Only the most recently pressed button shows in reverse video 3 o end the test and return to the DIAGNOSTIC MENU press Menu twice C 42 Doc 031935 01 4 04 C Display Screens C 9 9 Code Versions Screen Displays revision numbers for the current digital signal processor DSP code and syringe operating codes Help Prompt Figure C 32 Code Versions Screen Doc 031935 01 4 04 C 43 AutoSelect AS50 Autosampler C 10 Print Menu When a printer with a serial RS 232 interface is connected the PRINT MENU can be used to print a schedule a method
208. ultaneous Injection Mode On the SCHEDULE screen see Figure E 10 specify the method number of the simultaneous injection method and enter the injection volume The specified injection volume is loaded into the sample syringe and then delivered equally to the two sample loops Help Prompt Figure E 10 Schedule Screen Simultaneous Injection Mode 5 mL 1000 to 5000 uL 1000 uL 10 mL 1000 to 8000 uL 1000 uL Table E 1 Injection Volumes for Simultaneous Injections Doc 031935 01 4 04 E 9 AutoSelect AS50 Autosampler E 5 Setting Up Chromeleon This section provides an overview of the tasks required to set up Chromeleon to control simultaneous injections For detailed instructions on installing Chromeleon and connecting to the PC refer to Installing the Chromeleon Chromatography Management System with a Dionex Ion Chromatograph IC Document No 031883 The manual is available on the software CD and the Dionex Reference Library CD 1 Install Chromeleon on the PC start the Chromeleon Server and install the software license 2 Connect the 550 and both IC systems to the PC Turn on the power to each system and the AS50 4 Add a timebase in the Chromeleon Server Configuration program that includes both systems and the AS50 5 Assign a unique name to each device in the timebase See Section E 5 1 for details 6 Save the configuration 7 Start Chromeleon and connect to a Control panel Example C
209. using the following buttons e To select from a list of predetermined options press Select A or Select V repeat to cycle through the choices To enter a numeric value press the numeric buttons e To cancel an entry that is in progress and restore the previous value press Delete e To return a field to its default value if an entry is not in progress press Delete 3 IMPORTANT Press Enter or a cursor arrow button to confirm the entry Doc 031935 01 4 04 3 5 AutoSelect AS50 Autosampler 3 3 Default Operating Parameters The following table lists the factory set values for AS50 operating parameters Operating Screen Method Sample Prep Method Setup Method Timed Events Schedule System Parameters Flush Prime Parameter Step Cycle Time Sample Needle Height Column Temperature Wait for Temp Stable Time Init Inject Valve CSV TTL Relays Time 0 00 Inject Valve CSV TTL Relays Missing Vial Action Vial Start End Injections per Vial Injection Volume Method Number Wait Syringe Speed Cut Segment Volume Inject Port Volume Air Bubble Volume Flush Volume Flush Reservoir Flush Syringe Prime Volume Prime Reservoir Prime Syringe Default Value 1 Wait step only Off 2mm Off No Load for simultaneous injection both valves at Load position A CSV option not currently available All off 0 Inject for simultaneous injection both valves at Inject position A CSV o
210. utoSelect AS50 Autosampler AS50 Autosampler Sample In AS50 Needle Seal Line po IC System Module 2 To Column To Waste From Pump IC System Module 1 To Column To Waste From Pump Figure E 4 Injection Valve Connections for Simultaneous Injection Valves Installed in the IC System Modules Doc 031935 01 4 04 E Simultaneous Injections E 2 2 Connecting to Valves Installed in the AS50 1 Route the outlet lines from the pump and column in both IC systems through the slot on the right side of the AS50 see Figure E 5 Valve 2 Lines from column and pump in system 2 Lines from column and pump in system 1 Figure E 5 Routing Pump and Column Lines AS50 Injection Valves 2 Connect the pump outlet line from system 1 to port P 2 on the bottom injection valve valve 1 and connect the column outlet line from system 1 to port C 3 on valve 1 see Figure E 6 3 Connect the pump outlet line from system 2 to port P 2 on the top injection valve valve 2 and connect the column outlet line from system 2 to port C 3 on valve 2 see Figure E 6 Doc 031935 01 4 04 E 5 AutoSelect AS50 Autosampler Sample In Seal Line AS50 Needle Valve 2 To Column in System 2 To Waste ko From Pump in System 2 Valve 1
211. y controlling system functions starting the pump starting the detector method etc The syringe speed to use Select the default value of 5 for water more viscous liquids require slower speeds Values ENABLED DISABLED 1 slowest to 5 fastest default 4 C 24 Doc 031935 01 4 04 C Display Screens Screen Field Description Values CUT For partial loop injections specifies the volume to 0 to 30 uL SEGMENT cut from each side of the sample during the default 10 VOLUME injection process See Section 3 11 5 for details about injection types INJECTPORT The volume of tubing between the inject port and 1 to 999 uL VOLUME injection valve This represents the amount of extra default 25 liquid to be dispensed when loading the loop in order to accurately position the sample This value is determined at the factory and should not be changed unless a new needle seal assembly is installed SRS POWER Specifies whether to switch the power from ENABLED SWITCHING SRS to the other at the same time that the column DISABLED switching valve position is switched Note The column switching valve option is not currently available for the AS50 USB Doc 031935 01 4 04 C 25 AutoSelect AS50 Autosampler C 6 6 Inject Port Alignment Screen Use the INJECT PORT ALIGNMENT screen to align the sampling needle in the inject port See Section 5 7 for detailed instructions Help Prompt Figure

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