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Operation Manual - ASU Shared Resources

1. Fig 6 Leica CM1950 Cryostat 1 2 i The instrument must be transported in an upright position The cover 17 now forms a ramp on which the instrument can now be rolled down off the transport pallet Do not push the device by its hood 16 Use the transport grip points 15 The rollers 14 must run over the cover hinges 2 at the front and rear Danger of tipping Carefully roll the instrument backwards from the pallet Push the instrument to the installation location on the castors 14 2 Safety and Design 23 Safety Features The instruction manual includes important information related to the operating safety and maintenance of the instrument The Operating Manual is an important part of the product and must be read carefully before startup and use and must always be kept near the instrument If additional requirements on accident prevention and environmental protection apply in the country of operation this Operating Manual must be supplemented by appropriate instructions to ensure compliance with such requirements The instrument is equipped with the following safety devices an emergen cy stop switch motorized instruments only a handwheel lock and center ing system motorized instruments only knife guard on the blade and knife holder and a blade ejector To prevent adverse health effects from UV radiation the UV disinfec tion cycle can be started only after the sliding window has been pr
2. 1 2 Qualification of Personnel The Leica CM1950 may be operated by trained laboratory personnel only All laboratory personnel designated to operate the instrument must carefully read the present instruction manual prior to starting work with the instrument i Despite chemical and or UV light dis infection personal safety precautions as per the applicable laboratory regu lations must still be taken i e safety goggles gloves laboratory coat and mask must be worn This type of disinfection reduces the number of germs by at least 99 99 1 3 Designated Use The CM1950 is a high performance cryostat with an encapsulated microtome and separate speci men cooling It features a UV disinfection system an optional integrated extraction system for sec tion waste and an optional motor for motorized sectioning The cryostatis designed to produce frozen sections for biological medical and industrial applications The CM1950 is suitable for in vitro diagnostic IVD applications The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in this manual Any other use of this instrument is considered as improper operation 1 4 Instrument Type All information given in this instruction manual applies only to the instrument type indicated on the title page A name plate indicating the instrument serial number is attached to the b
3. e nsertthe pin 1 of the handwheel shaft into the hole 2 of the handwheel e Tighten the screw 4 using the No 6 Allen key e Place the protective cap on the screw 4 Fig 14 lo dismount proceed in reverse order 22 Operating Manual V 1 1 05 2007 6 Installation 6 3 1 Locking Unlocking the Handwheel Only rotate the handwheel when the refrigeration system is on and 12 o clock the cryochamber is cold position Always lock the handwheel prior to manipulating the knife or specimen changing the specimen or taking a break 6 o clock position To lock the handwheel move its handle to the 12 or 6 o clock position Press the lever 1 fully outward gently rock the handwheel until the locking mech anism clicks into place noticeably Fig 15 To release the handwheel press the lever 2 on the handwheel toward the cryostat housing Fig 16 Centering the Handwheel Optional An important safety device on the cryostat is the feature for center ing the handwheel in motorized sectioning mode To do so pull outthe handwheel s handle and position itin the middle of the handwheel The handle automatically engages in this position Fig 17 6 3 2 Installing the Footswitch Dummy Instruments with Cutting Motor e The footswitch dummy must be installed on the outer right side of the instrument see page 16 General overview if no footswitch optional is used Ifthe red LED in the
4. 8 1 Preparing Cutting Tools Specimen Discs and Preparation Aids The knives are extremely sharp Handle with care Never try to catch a falling knife e Place working materials such as the blade box or knives in the knife case brush pincers or preparation needles and where applicable specimen discs into the cryostat chamber e Thenecessary tools and preparation aids can be precooled on the optional movable shelf making them available at all times in a conve nient position for the user e n addition specimen discs can be precooled and stored in the storage system see page 26 Fig 21 For installation of knife blade holder and installation in the chamber refer to optional accessories on page 50 The instrument must be switched on at least 5 hours before the planned use The circuit breaker is used as power switch The switch must be in the top position for switching on and in the bottom position for switching off The switch must be accessible without obstruction e Close the sliding window Fig 37 i To avoid frost formation always put the cover on the quick freeze shelf Always cover the quick freeze shelf during breaks and overnight 36 Operating Manual V 1 1 05 2007 8 Working with the Instrument 8 3 Configuring the Parameters The instrument must be switched on at least 5 hours before the planned use Leica CM1950 Cryostat Turn the lamp on or off This button activa
5. Leica CM 1950 Cryostat C Operating Manual Leica CM1950 V 1 1 English 05 2007 Always keep this manual near the instrument Read carefully prior to working with the instrument eca MICROSYSTEMS 1 NOTE The information numerical data notes and value judgments contained in this manual representthe current state of scientific knowledge and state of the arttechnology as we understand itfollow ing thorough investigation in this field We are under no obligation to update the present manu al periodically and on an ongoing basis accord ing to the latest technical developments nor to provide our customers with additional copies updates etc of this manual For erroneous statements drawings technical illustrations etc contained in this manual we ex clude liability as far as permissible according to the nationallegal system applicable in each indi vidual case In particular no liability whatsoever Is accepted for any financial loss or consequen tial damage caused by or related to compliance with statements or other information in this manual statements drawings illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products Seica MICROSYSTEMS Published by Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Phone 49 6224 143 0 Fax 49 6224 143
6. 18 x 18 x 6 mm 64 Operating Manual V 1 1 05 2007 11 Optional Accessories Footswitch for use with motorized instruments only Adjustable support individually height adjustable foot rest with 5 set tings Brush shelf for use with blade holder CE Fig 65 Storage system movable for installation in front area of cryostat for cold storage of preparation tools Leica CM1950 Cryostat 65 12 Maintenance and Cleaning 12 1 General Maintenance The microtome is virtually maintenance free To ensure a smooth operation of the instrument over several years we recommend the following e Atleastonce a year have the instrumentinspected by a qualified service engineer authorized by Leica e Enter into a service contract atthe end of the warranty period For further information please contact your local Leica service center e Clean the instrument daily e Every day remove frozen section waste from the cryostat using a cold brush or use the optional extraction device e Remove the section waste tray for emptying e Remove the storage shelves and the brush shelf for cleaning e Remove the closed sliding window from the front by gently lifting it see p 63 Replacing the Fluorescent Lamp cleaning and disinfecting Use only disinfectants listed in this manual such as Leica Cryofect alcohol or other common alcohol based disinfectants i Do not use organic solvents or any other aggr
7. Part 3 2 Limits Limits for harmonic current emissions e EN 61000 3 3 1995 A1 2001 Part 3 Limits Section 3 Limitation of voltage changes voltage fluctuations and flicker in public low voltage supply systems for equipment with rated current lt 16A per phase Electromagnetic compatibility EMC e EN 418 1992 e EN 1037 1995 Emergency stop equipment Safety of machinery Principles for design Prevention of unexpected start up In addition the following in house standards were applied e DIN EN ISO 9001 2000 Quality management system requirements Leica Biosystems Nussloch GmbH Postfach 1120 D 69222 Nussloch October 11 2007 President Biosystems Division 15 Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce dure based on the Leica in house testing standards and that the productis faultless and complies with all technical specifications and or agreed characteristics warranted The scope of the warranty is based on the content of the concluded agree ment The warranty terms of your Leica sales organization or the organiza tion from which you have purchased the contractual product shall apply exclusively Technical service information If you require technical service or replacement parts please contact your Leica sales representative or dealer who sold the product Please provid
8. eee 0477 40045 Specimen disc 55mm assembly eee 0477 40046 Specimen disc 80x50 mm assembly scese 0477 43714 O ring blue 10 pieces Ii 0477 43247 O ring red 10 pieces 20 mm nnne 0477 43248 O ring blue 10 pieces 30 mm enne 0477 43247 O ring red 10 pieces 30 mm nnns 0477 43248 O ring blue 10 pieces 40 mm nnne 0477 43249 O ring red 10 pieces 40 mm nnne 0477 43250 O ring blue 10 pieces 55 mm enne 0477 43251 O ring red 10 pieces 55 mm nnns 0477 43252 Blade holder base complete 0477 40351 Blade holderextension CE BB complete 0477 43005 Insert for low profile blades for blade holder CE complete 0477 42498 ne 047740387 Anti roll plate 70 mm 50 UM spacer eee 0477 42491 Anti roll plate 70 mm 100um spacer eee 0477 42492 Anti roll plate 70 mm 150um spacer eee 0477 42493 Glass insert 70 mm polished ne 0477 42497 Knife holder base assembly ee 0477 42359 Knife holder CN assembly enne 0477 42358 Knife support CN short ssi Eben Erin Dn c patr imr IEEE RECO 0 0477 42390 Knife support EE ae 0477 42370 Knife holder attachment CNZ assembl
9. sresanrsrrrerervrnervenrevervevervesssvervevssvesvsversevesvesennes 0183 40426 1 Allen EN 0222 10050 1 Allen key No 2 5 0222 04137 SUE SITE Ge 0222 04138 1 Allen key No 4 0 sissconsesinnvsncnsinnevationanennteienctusteassidvucatvdivetentnedtietasensduasaawn 0222 04139 1 Allen key with dome head No 4 0 0222 32131 AEE NO DI rnani N 0222 04140 21 key uM SIS M NO NETT 0194 04760 EE NO O O asserere eerta nd 0222 04141 1 Single head wrench No 13 nnne 0330 33149 1 Single head wrench No 16 emmereservererveresvsvevervevervrveserevesseresseresersene 0330 18595 DONE er ND UT 1 bottle of cryostat oil type 407 50 ml nne 0336 06098 1 bottle of OCT freezing compound 125 ml eee 0201 08926 1 pair of safety gloves size M for cryosectioning 0340 29011 1 user manual plus language OD EE 0708 37115 Note for the Japanese version 100V 50 60 Hz 1 pair of safety gloves size S 0340 40859 is included Basic instrument WITHOUT motor and WITH extraction Standard scope of delivery as above plus 1 accessory set extraction Luanda beer iter SPI a oda i 0477 43300 Hose adapter EEA siini 0477 40293 TLS Adapter EE odii edat 0477 40294 eo nozzle NNI RR RR 0477 40295 Se EE a SE 0477 43302 Silicone Stopper ene 0477 43304 gt Chamber suction nozzle ceste ro ortu reden icto atentos tesbns tds 0477 4377
10. 200 eMail histo infoQleica microsystems com Internet http www histo solutions com Leica CM1950 Cryostat These are determined only by the contract provi sions agreed between ourselves and our customers Leica reservesthe rightto change technical spec ifications as well as manufacturing processes without prior notice Only in this way is it possible to continuously improve the technology and man ufacturing techniques used in our products This documentis protected under copyrightlaws All copyrights to this documentation are held by Leica Biosystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photocopy microfiche web cam or other methods includ ing any electronic systems and media requires express prior permission in writing by Leica Biosystems Nussloch GmbH For the instrument serial number and year of man ufacture please refer to the name plate at the back of the instrument Leica Biosystems Nussloch GmbH Table of contents m Important Information sse nennt na 6 1 1 Symbols Used in This Manual and Their Meaning eene 6 1 2 Qualification of Personel jscusscevacaatesdnonsatnctabrancpdsadeandinineaiesaradinantateeidnadeantninsnnaheeinoisteiaonseracstagnas 6 BENENE E WITNESS CTI IH ERI ON UE TCI ED TI EDI IUE 6 ES ENE NE EE 6 Safety E sse tnnt tentent nn 7 ss AS ed SE DE PU MUR dU ectag
11. 25 bar 3 at 230 V 50 Hz 140 g 5 g refrigerant R 404A at 100 V 50 60 Hz 110 g 5 g refrigerant R 404A 0 4 I alpha 22 Kyodo No 15 min CM1950 60 Hz 0 C to 35 C 5 K adjustable in 1K increments ambient temperature of 20 C approx 5h approx 8h 690 W 25 bar 3 300 g 5 g refrigerant R 404A 0 6 I EMKARATE RL22S ICI Yes hot gas defrost Selectable time 1 defrost in 24 h or manual hot gas defrost 12 minutes at 5 C chamber temperature 42 C 4 5 K at chamber temp 35 C 1542 Manual hot gas defrost 2 17 K at chamber temp of 35 C 10 to 50 C 3 K 320 W 25 bar 3 at 120 V 60 Hz 110 g 5 g refrigerant R 404A 0 4 I alpha 22 Kyodo No 15 min Refrigerant and compressor oil must be replaced by qualified authorized service personnel only Q acc to CECOMAF liquid temperature 45 C evaporation temperature 25 C Leica CM1950 Cryostat 15 4 Standard Delivery 16 Basic instrument WITHOUT motor WITHOUT extraction in the specific voltage variant 1 handwheel manual enn 0477 41346 5 specimen discs MN 0477 40044 1 section waste TER Rio nEe rhon auc Gesn pe cRR I EINE TORUM DE DXNEEE DIXERINT EE 0477 40062 1 position holder for freeze shelf se 0477 40080 1 freeze shelf MEET 0477 43763 IE Er i T 0436 43463 TS en 0183 28642 1 Leica brush w magnet
12. E STOP field in control panel field 3 is illuminated either e emergency stop function active or e footswitch dummy optional footswitch connected incorrectly or not at all Fig 18 Leica CM1950 Cryostat 23 6 Installation 6 4 Connection to Power Supply System After transporting wait at least 4 hours before turning the instrument on This waiting period is necessary to allow the compressor oil which may have been displaced during transport to re turn into its original position Furthermore any condensation that has formed during this time due to temperature fluctuations must be allowed to dry completely Failure to comply with this can cause severe damage to the instrument During the start up of the compressor the nominal voltage must not drop below the values specified in the Technical Data Please note that the compressor requires a start up current between 25 and 35 A Therefore the electric circuit at the installation site must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument Failure to comply with the above will cause severe damage to the instrument e Check mains voltage and mains frequency to comply with the specifica tion on the type plate e Donotconnectany other consumers to this electric circuit Never connect the instrument to a power socket that does not have a protective conductor terminal Only for instruments s
13. chamber temperature select higher temperature Remove cause select lower temperature Trim the specimen parallel Increase section thickness Reposition anti roll plate Align correctly set correct angle Use different part of the knife Clean with dry cloth or brush Replace anti roll plate Use different part of the knife Readjust correctly Readjust correctly Operating Manual V1 1 05 2007 9 Troubleshooting Problem Ridged sections Chatter during sectioning Condensation on anti roll plate and knife during cleaning Anti roll plate damaged after adjustment Thick thin sections Leica CM1950 Cryostat Causes Knife damaged Edge of anti roll plate damaged Specimen insufficiently frozen onto the specimen disc Specimen disc not clamped tightly Knife not clamped tightly enough Specimen has been sectioned too thickly and has detached from disc Very hard inhomogenous specimen Blunt knife Knife profile inappropriate for specimen cut Incorrect clearance angle Brush forceps and or cloth too warm Anti roll plate too high above the knife edge The adjustment was carried out in the direction of the knife Temperature incorrect for the tissue cut Knife profile inappropriate for the specimen cut Ice buildup on the knife back Handwheel speed not uniform Knife not clamped tightly enough Specimen disc not clamped tightly enough Cryocompound applied to cold speci
14. in the VAC switch goes off to prevent icing To continue working you now have to reactivate the VAC 2 Trimming with Extraction Brush Technique finger rest installed Remove the silicone stopper E from the filter cover and keep itin a safe place Insert the extraction hose with the black adapter Fasten the suction nozzle to the pressure plate using 4 magnets on the rear side of the nozzle as far as possible towards the blade The tension acting on the hose can be minimized by turning the red ring Fig 28 on page 28 clockwise so that the suction nozzle presses againstthe pressure plate Check that the suction nozzle is seated optimally by gently turning the handwheel Operating Manual V 1 1 05 2007 8 Working with the Instrument Activate trimming mode selectthe trimming thickness Switch on the VAC and select a low extraction level between 1 and 2 Start trimming by manually moving the handwheel or press the RUN STOP and ENABLE buttons simultaneously to start motorized sectioning Use a brush to apply the section to a precooled specimen slide then warm it from below using your finger Move the knife guard forward after removing the section Remove the knife or disposable blade using the blade ejector Insert the knife into the knife case 8 4 3 Cutting with Extraction Anti roll Guide installed Leica CM1950 Cryostat Shut off VAC LED in VAC button goes out Switch from trim
15. the installation site Call technical service Check lamp and replace it if necessary Call technical service Replace UV tube following the manufacturer s instructions Contact technical service and follow the instructions given 51 10 Temperature Selection Chart in minus C Tissue type Chamber temperature Specimen head temperature Spleen 15 C to 20 C 11 C Liver 10 C 20 C 15 C off to 15 C Intestine 10 C 20 C 15 C A off to 20 C E 20 C Heart 10 C A 20 C E 20 C to 30 C 15 C off to 20 C Ovaries 10 C E 20 C 15 C off to 15 C Fallopian tubes 10 E 20 C 15 C off to 15 C Kidney 10 C 20 C 15 C A off to 15 C 20 C 20 C Muscle 18 C to 20 C 15 C Skin with fat 19 C 32 C to 40 C Hard fat 19 C 21 C to 25 C Stomach 10 C 20 C 15 C off to 15 C Brain 15 C 10 C E A blocked E complete The temperature values given above are based on long term experience however these are only approximate values as any tissue may require particular adjustments 52 Operating Manual V 1 1 05 2007 11 Optional Accessories 11 1 Ordering Information Leica CM1950 Cryostat Specimen disc 20mm assembly ee 0477 43739 Specimen disc 30mm assembly ee 0477 40044 Specimen disc 40mm assembly
16. the sliding window must be completely closed e Press UVC key once briefly to start the 30 min mode e Press UVC key once for a longer time UVC key to activate deac approx 4 sec to start the 180 min mode tivate the disinfection cycle and or to acknowledge in Fig 35 terruption of a disinfection cycle i The germicidal UV lamp is a simple and safe disinfection device which considerably reduces the risk of infection Nevertheless UV light disinfection cannot replace chemical disinfection of the cryochamber at regular intervals UV light disinfection is effective only on those surfaces that are directly illuminated Specimens and section waste must be thoroughly removed from the cryochamber first e g using the optional vacuum extractor or a paper towel soaked with Cryofect or alcoholic disinfectant Before UV disinfection move the anti roll guide to the side to allow complete disinfection e Leica Cryofect Is not available in all countries i i Leica CM1950 Cryostat Opening the sliding window cancels the disinfection cycle Press the UVC key to acknowledge this When the keypad lock is activated via the key button the UV lamp can be shut off only by opening the glass as the UV keys are locked The cancellation can be acknowledged only if the keypad lock is disabled Only then can the UV lamp be switched back on 35 8 Working with the Instrument
17. wise so that the suction nozzle presses against the pressure plate I Fig 39 an e Check that the anti roll guide is parallel and correctly adjusted sect Readjust if necessary see page 58 e Activate trimming mode e Select the trimming thickness VAC e Switch on the VAC and select a low extraction level between 1 and 2 e Start trimming by manually moving the handwheel or press the RUN STOP and ENABLE keys simultaneously to start motorized sectioning Leica CM1950 Cryostat 43 8 Working with the Instrument For motorized sectioning always begin at low speed for safety reasons Optimize the vacuum settings if necessary Press the VAC button to enable the vacuum extractor The LED in the VAC button is lit while the extractor is on Press the button again to disable it Use the knob to adjust the intensity of the vacuum Optimal area for trimming and sectioning Trimming Handwheel position 12 6 o clock valve open Handwheel position 6 12 o clock valve closed Cutting Handwheel position 12 3 o clock valve open all the way Handwheel position 3 6 o clock valve half open Handwheel position 6 12 o clock valve closed Optimal area for extraction from the chamber To clean the chamber turn the knob to the red range If the handwheel is not moved for approx 5 sec the valves close and the fan remains on If the handwheel is moved for approx 1 min the valves close the fan shuts off the LED
18. with Extraction Anti roll Guide Installed sse 45 HR eR Ea EEA 48 91 Problems Durno EN EEE 48 Temperature Selection Chart in minus C ou sse 52 Optional Accessories ccrte arret oder tese bUE PIU surdi HE Aeneas 53 LI Ordering UA LOTMA CIO Mi sessies rannen ar d ird Er dira mcr Prat E RU ERE 93 Maintenance and Cleaning 5 5 5 5 seen 66 121 General Maintenance usse teilte ERO nubi pL Xe RE DRE RIS YR UL RM rubei kn dE UU EY ERR tmp CPI TED 66 l2 SSUES EE EEE ON 67 12 3 Replacement of the Lamp UVC Lamp eeresvrrrerorververvrververvesrrvervesvesesvesvesvesesvesvesvevssvesvervevenne 68 Decontamination Certificate master corrrvrrrrvrrrrererrererrererserersererserersererrererserersererserernn 70 EC Declaration of Conformity seen 72 Warranty and Service seen nennen tenente 13 Peoles Republic of China 5 sse nennen nnne tanen 74 Leica CM1950 Cryostat 5 1 Important Information 1 1 Symbols Used in This Manual and Their Meaning Warnings appear in a gray box and are marked by a warning triangle Notes i e important user information appear in a gray box and are marked by an in formation H symbol Caution UVC radiation AN 5 Fig 5 Figures in brackets refer to item num bers in drawings or to the drawings themselves
19. 5 A eff 15A M3 2 1900 J s CE c CSA us 700 mm 835 mm 850 mm 1215 mm 1025 mm 193 kg 185 kg 183 kg 175 kg 165 kg 145 kg 18 C to 35 C 5 C to 55 C max 60 non condensing lt 60 Operating Manual V 1 1 05 2007 3 Technical Data Refrigeration system Cryochamber Temperature range Cooling time to 25 C Cooling time to 35 C Refrigeration capacity D Cut out pressure Safety factor Refrigerant Compressor oil Defrosting of cryochamber Automatic defrosting Programmable Defrosting intervals Defrost time Automatic shutoff Defrost Quick freeze shelf Minimum temperature Number of freezing stations Defrost Peltier element Number of freezing stations Max temperature difference Specimen cooling Temperature range Refrigeration capacity Cut out pressure Safety factor Refrigerant and quantity Compressor oil Defrosting of specimen head Automatic defrost Manual defrost Defrost time CM1950 50 Hz 0 C to 35 C 5 K adjustable in 1K increments at ambient temperature of 20 C approx 5h approx 8h 690 W 25 bar d 300 g 5 g refrigerant R 404A 0 6 EMKARATE RL22S ICI Yes hot gas defrost Selectable time 1 defrost in 24 h or manual hot gas defrost 12 minutes at 5 C chamber temperature 42 C 5 K at chamber temp 35 C 15 2 Manual hot gas defrost 2 17 K at chamber temp of 35 C 10 to 50 C 3 K 320 W
20. 9 Set of filters b pieces ssssssseennnnnnnnnnnnnnnnnnnnnne 0477 43792 Compare the delivered components with the parts list and your order Should you find any discrepancies please contact your Leica sales office without delay i A choice of different knife holders is available for the CM1950 Operating Manual V 1 1 05 2007 4 Standard Delivery Basic instrument WITH motor WITHOUT extraction in the specific voltage variant 1 handwheel motorized eeenennnnnnnnnnnn nnne 0477 41347 5 specimen discs IN 0477 40044 1 section waste Tray EEE nance 0477 40062 1 position holder for freeze shelf e 0477 40080 1 freeze shelf re 0477 43763 Boe SOL E ean A E E E AAE E E E ai 0436 43463 ee EE 0183 28642 1 Leica brush w magnet senes 0183 40426 1 Allen key PIN RINT 0222 10050 1 Allen key NO 2 5 0222 04137 SPESSO CUM NO 2 0 arn E E 0222 04138 1 Allen key EEE qu ritmi rune 0222 04139 1 Allen key with dome head No 4 0 ne 0222 32131 1 Allen key No 5 0 0222 04140 ME with handle NO D MR TT 0194 04760 MN 0222 04141 1 Single head wrench No 13 nnne 0330 33149 1 Single head wrench No 16 eene 0330 18595 TiVO VALE 5 OE eem 1 bottle of cryostat oil type 407 50 ml nne 0336 06098 PFIN Verv 0443 30420 1 bottle of OCT freezing compound 125 m
21. 950 Cryostat 59 11 Optional Accessories Knife holder CN with anti roll guide Moving the clamping chucks The specimen disc 50x80mm is suitable only for section thicknesses up to approx 5um due to the large specimen size Thelarge specimen disc 80x50 mm should pref erably be used with knife holder CN and the 16 cm C profile steel knife The clamping jaws are factory installed in the knife holder with a clearance of 64 mm If neces sary both clamping chucks can be offset with a clearance of 84 mm Perform the following steps Use a3 mm Allen key to loosen the screw over the clearance angle adjustment 1 and remove the segment arch 2 from the knife holder base Use a3 mm Allen key to loosen the screws 4 on the underside of the segment arch Carefully pull the clamping jaw 5 on the right upward and insert it in the neighboring hole 6 Tighten the screws from the underside of the segment arch Repeat on the left side Insert the long knife support 3 on the side over the knurled screw 3a so thatthe recess faces the user turn the height adjustment knurled screws until the lower stop Is reached The knife can now be inserted from the side and its height adjusted via the knurled screws 3a instance Never work with only one clamping jaw as this does not ensure the stability required for the sectioning process Also a long knife will not be sufficiently
22. Dirt on knife back Power plug not properly connected Defective fuses or circuit breaker has triggered Moisture on the underside cause the specimen to freeze to the freezing shelf or specimen head Remedies Use different part of the knife select correct section thickness set correct angle Dry microtome Prepare new specimen Cool down anti roll plate or reposition correctly Remove fat from anti roll plate Fix correctly Remove rust Cool down anti roll plate Increase temperature and wait Remove cause Replace anti roll plate Clean Check if properly connected Replace fuses or switch circuit breaker back on If not possible call technical service Apply concentrated alcohol to the contact point Operating Manual V1 1 05 2007 9 Troubleshooting Problem No or insufficient refrigeration of the cryochamber sliding window collects condensation No or insufficient refrigeration of the specimen Lamp does not work Both disinfection LEDs flash alternately Leica CM1950 Cryostat Causes Cooling system or electronic drive defective Air humidity and room temperature too high Cooling system or electronic drive defective Lamp defective Switch defective UV radiation provided by UV tube no longer sufficient Image of an open ended wrench appears due to fault to be rectified Remedies Call technical service Comply with the requirements for
23. T No Quantity A Yes This equipment has not been in contact with unfixed biological samples B 1 This equipment has been exposed internally or externally to hazardous materials as Yes No Indicated below Please provide further detail here Blood body fluids pathological samples BEN Other biohazards RT Chemicals substances hazardous to health No Other hazards This equipment has been cleaned and decontaminated If yes give details of the method Please provide further detail here If no please indicate why not Such equipment must not be returned without the written agreement of Leica Microsystems 10 Operating Manual V 1 1 05 2007 13 Decontamination Certificate master 3 Yes No The equipment has been prepared to ensure safe handling transportation Whenever possible please use the original transportation case box Important to avoid refusal of shipment Place one copy in the unit prior to packaging or hand it over to the service engineer Customer assumes all responsibility for the immediate return shipment of articles sent to Leica without proper decontam ination documentation If you have any further questions please call your local Leica organization Leica Internal Use f applicable note corresponding Job and RAN RGA Number Job Sheet No SU Return Goods Authorisation BU Return Authorization Number Signature Date Institute Department Name Address Position eMail Phone F
24. ack ofthe instrument aria E 1900 V CHaopik hung pe e E aimi R404 A eer Hogtsgi leica Mic VES semi Nu issloch CmbH 26 Fig 1 m AESA Operating Manual V 1 1 05 2007 2 Safety and Design 2 1 General Safety Instructions The CM1950 is a cryostat with an encapsulated microtome and separate specimen cooling It Is primarily used for work in the area of fast cut diag nostics The displays and instrument controls are easy to operate due to their largely self explanatory symbols LED displays make it easy to read The cryochamber is made of seamlessly welded high quality stainless steel thatis free of difficult to access corners and thus easy to clean and disinfect This instrument has been built and tested in accordance with the following safety regulations on electrical measuring control regulating and labora tory devices In order to maintain this condition and to ensure safe opera tion the operator must observe the instructions and warnings contained in this instruction manual For current information about applicable standards please refer to the CE declaration of conformity on our Internet site WWW histo solutions com 22 Unpacking and Installation e To ensure proper function of the instrument it must be set up with a minimum distance on all sides from walls and furniture see Site Requirements p 20 e The instrument may only be transported in an uprig
25. acsimile Seica MICROSYSTEMS Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 69226 Nussloch Germany Phone 49 0 6224 143 0 Fax 49 0 6224 143 200 www leica microsystems com Leica CM1950 Cryostat 71 14 EC Declaration of Conformity Ce Leia EC Declaration of Conformity need We herewith declare in exclusive responsibility that the Leica CM1950 Cryostat was developed designed and manufactured to conform with the e Council Directive 2006 95 EC Low Voltage e Council Directive 89 336 EEC Appendix Electromagnetic Compatibility and e European council Directive 98 79 EC IVD including their amendments up to the date mentioned below The following harmonized standards were applied e EN 61010 1 2001 safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements e EN 61010 2 101 2002 safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment e EN 591 2001 Instruction for use for in vitro diagnostic instruments for professional use e EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements e EN 61326 2 6 2006 Electrical equipment for measurement control and laboratory use EMC requirements e EN 61000 3 2 2000 Electromagnetic compatibility EMC
26. amber remove all samples e Before defrosting the specimen head remove all samples Never leave samples in the cryochamber The instrument is not suitable for storing frozen specimens as the refrigeration dehy drates the specimens 2 1 Removal of the Microtome e The microtome is encapsulated and therefore does not require removal by the user 28 Maintenance Replacing the fuses e Turn the instrument off and disconnectthe power plug before replacing the fuses Use only the fuse types specified in chapter 3 Technical Data Failure to comply with this can cause severe damage to the instrument Replacing the fluorescent lamp UVC lamp e Turn the instrument off and disconnect the power plug before replacing the lamps It is possible to break the UVC lamp during replacement If this happens the lamp change must be completed by Technical Ser vice If any metallic mercury is released handle it carefully and dispose of it properly i If both disinfection indicator lights are blinking alternately the UV tube must be replaced 9 ZNSS Leica CM1950 Cryostat 13 3 Technical Data i All specifications related to temperature are valid only up to an ambient temperature of 18 C to 35 C and a relative humidity of no more than 60 Type Nominal voltage 10 Nominal frequency Power draw Max start up current for 5 sec Protective class Circuit breaker MDA made
27. by Schurter Pollution degree 1 100 V AC 50 60 Hz 1900 VA 35A eff 15A M3 2 Overvoltage installation category ll Heat emission max Approval according to IEC 1010 UL 3101 Microtome Type section thickness range Trimming range Clinic Research Total specimen feed Vertical stroke Specimen retraction Maximum specimen size Cutting speed Specimen orientation For additional details see p 32 Electric coarse feed 1900 J s CE c CSA us Rotary microtome encapsulated 1 to 100 um 10 40 um 1 600 um 25 mm I mm 59 mm 0 5 mm 20um can be deactivated 50 x 80mm Slow 0 50 strokes min Fast 0 85 strokes min Vmax 85 90 strokes min 8 x y axis Slow 300 um s Fast 900 um s For Site Requirements refer to item 6 1 page 20 Lamp 50 60 Hz Version Osram 14 DULUX L 18 W 840 2 120 VAC 60 Hz 1900 VA 35 A eff 15A M3 2 1900 J s CE c_CSA_us Cryostat Dimensions Width w o handwheel Width with handwheel Depth cabinet only Height total Working height armrest Weight Weight w motor and suct Weight w motor w o suct Weight w o motor w suct Weight w o motor w o suct Weight w specimen head cool Weight w o specimen head cool General information Operating temperature range Temperature range during storage Relative humidity Storage humidity 3 230 V AC 50 Hz 1900 VA 2
28. d e Fold the anti roll guide away and to the side Insert a precooled knife or blade e Approach the specimen with the knife or blade holder e To do so open the clamping lever of the base approach the specimen and close the lever again e Open the lever of the orientation Orient the specimen move it into a favorable position relative to the knife blade and close the lever again e Approach the knife or blade holder using the coarse feed buttons and gentle movements of the handwheel If the sections are cracked the specimen head temperature is too cold Set a warmer temperature If the sections smear the specimen head temperature is too warm Set a colder temperature 42 Operating Manual V 1 1 05 2007 8 Working with the Instrument 8 12 Trimming with Extraction Anti roll Guide Installed e Remove the silicone stopper from the filter cover and keep it in a safe place e nsertthe extraction hose with the black adapter e Fold the anti roll guide to the side and fasten the suction nozzle to the pressure plate see mark Fig 39a using 4 magnets on the rear side of the nozzle e Fold the anti roll guide back into position Fig 39b Fig 39c Ensure that the hose with the nozzle is not installed against its natural cur vature on the pressure plate of the blade holder The tension acting on the hose can be minimized by turning the red ring Fig 39 top right clock
29. d secure it in the bore 3 on the base 2 using the No 4 Allen key e Push the knife holder base 2 on the dovetail guide 4 from the front and tighten it using the clamping lever 5 To move the base open the clamping lever only a little to prevent acciden tal sliding in the direction of the specimen head e fthe clamping distance is not sufficient the clamping lever 3 can be moved To do so pull the lever out and move it to the next position When removing the knife holder base 2 from the refrigerated cryostat chamber hold it by the grip points a front and rear to keep your fingers from freezing Leica CM1950 Cryostat 21 6 Installation 65 7 Inserting Changing the Bacteria Filter Fig 26 6 5 8 Assembling the Filter Bag Fig 27 Fig 28 28 The holder for the bacterial filter optional is visible in the front of the instrument The filter must be disposed of accord ing to valid laboratory specifications Toinsertthe filter hold it with one hand press on the right of the socket then guide the filter into the tube from the left To change the filter follow the opposite proce dure press the filter to the right then pull it to the left and out of the tube The filter must be changed approx every 3 months we recommend writing the date on the filter using a marker Set the mark A of the extraction opening 1 and pull it out Plug the fi
30. e rest ruler for low profile to blade holder with finger rest alates e Screw off the anti roll guide e Unscrew left Allen screw using 2 5 mm Allen key and remove base of anti roll guide The knife guard is folded away in the detail view Knife guard folded away e Attach the finger rest 6 from the left tighten Four possible fr the hexagon head screw using the No 2 5 settings n F key be careful of the blade ejector If you are working with the brush technique the knife guard must he folded upwards 56 Operating Manual V 1 1 05 2007 11 Optional Accessories Inserting the blade into knife holder CE Fig 45 Anti roll guide system functions as a knife guard here Fig 46 Leica CM1950 Cryostat 1 Fold the anti roll guide system 4 to the left while doing so hold the lever 11 not the adjusting screw of the anti roll guide so that the height of the anti roll guide remains unchanged 2 Openthe clamping lever 10 by turning it coun terclockwise 3 Carefully insert the blade 9 from above or from the side between the pressure plate and the blade rest Ensure that the blade is insert ed so that it is centered 4 Rotate lever 10 clockwise to clamp 5 Fold the anti roll guide system 4 back to the side using the lever 11 Lateral shift for Blade Holder CE If the sectioning results are not satisfactory the knife holder can be shifted sideways in order t
31. e the following information Model name and serial number of the instrument Location of the instrument and name of the person to contact Reason for the service call Date of delivery Shutdown and disposal of the instrument Leica CM1950 Cryostat The instrument or parts of the instrument must be disposed of in compli ance with the local laws Dispose of UV tube separately 13 16 People s Republic of China Administrative Measures on the Control of Pollution Caused by Electronic Products dabimus Hazardous substances ARTE Name of the part v K 553 NUR SUDO FORT AAK Po Gg Ca Cr B BIE FT Al Fa ER AZ printed circuit boards HAS tt electronic components NL EE mechanical parts tr sees opa components mes TT OT ANT Wasws 1 2 light sources o KmWiutieh mma wma m EET SJT 11363 2006 PER ENRE ERAT Indicates that the concentration of the hazardous substance in all materials In the parts is below the relevant threshold of the SJ T 11363 2006 standard x Ka id Gi ENE VEGAR KAR RESIT 11363 2006 H R TARRE R Indicates that the concentration of the hazardous substance of at least one of all materlals In the pans is above the relevant threshold of the SJ T 11363 2006 standard Note The actual product may or may not include in all the part types listed above 74 Operating Manual V 1 1 05 2007
32. ecreases the time continuously auto repeat function 38 Operating Manual V 1 1 05 2007 8 Working with the Instrument Programming the defrost cycle e Setthe beginning of the automatic defrost cycle The automatic defrost cycle takes place once within 24 hours It is set with the 7 buttons on the right of the panel with the clock symbol The two buttons are marked by a melting snowflake E e Briefly press the or button for indication of the beginning of the defrost cycle which has actually been set At the same time the LEDs between the indication of hours and minutes are flashing e Tochangethe beginning of the defrost cycle in steps of 15 minutes push the or key When the or button is pushed for more than 1 s the defrost time value increases or decreases continuously Before starting the defrost cycle remove all samples from the cryochamber e When the automatic defrost cycle begins the specimen head tempera ture adjusts to a temperature between 10 C and 5 C reduction of ice formation The specimen head cooling turns off This is confirmed by the flashing of the decimal points on the panelfor the specimen cooling The specimen cooling controlled to set value automatically turns back on after 4 hours once the chamber temperature varies by less than 5 K from the target temperature e f you want to turn the specimen cooling back on manually before the automatic activation sets in push
33. en head and real time display fields refer to the chapter on Working with the Instrument on page 37 of this Operating Manual Leica CM1950 Cryostat 31 1 Instrument Controls Control Panel Field 2 Electric Coarse Feed Sectioning and Trimming Thickness Display Illuminates in yellow when the i For trimming values above a sec specimen is in tion thickness of 200 pm the dis retraction LED for trim play flashes to expressly notify and section the user of thick sections thickness Toggle TRIM and display SECT LED active Rocking mode only in manual mode in rear area i e approx 12 3 o clock position of the handwheel rock handwheel back and forth a short distance Press approx 3 sec on or off appears for the retraction Toggle using the ne or key Move specimen head backwards Retraction GJ off 0 quickly to home position latched on 20um Move specimen head backwards slowly pressing In manual mode briefly retracts the specimen 20 jim For motorized sectioning the re traction value is fixed and cannot be changed see page 39 lower infobox In the off setting there is e Specimen head fast no retraction in manual WEN feed Specimen head forwards pressing briefly advances the specimen 20 pm automatic or rocking mode Fig 33 Setting section trim thickness Use the keys on control panel 2 Range of section thickness 1 100 u
34. esseseesansesseseneaees 0477 44307 Cryofect VPE Leica Cryofect 4x 350ml low temp disinfectant to 20 C sssseennne 0387 42801 Safety gloves size E 0340 29011 Sale OVS SIZE S NO TT 0340 40859 Footswitch dummy plug CM 3050 nee 0443 30420 Laboratory chair on sliders 8030442 ee 0710 34911 Footswitch assembly sosssesseeizvunirsibeonthskkel visvivti ula UU Er Mta aE pret rmm 0481 43397 Easy Dip staining container white sssrin 0712 40150 Easy Dip staining container pink enne 0712 40151 Easy Dip staining container green eene 0712 40152 Easy Dip staining container yellow ene 0712 40153 Easy Dip staining container blue eene 0712 40154 Easy Dip staining rack gray eene 0712 40161 Operating Manual V 1 1 05 2007 11 Optional Accessories Blade holder CE with anti roll guide for low profile NB and high profile BB Fig 41 Fig 41a Fig 42 Leica CM1950 Cryostat With blade ejector 1 With knife guard 2 Integrated lateral shift and stable base Clearance angle adjustment b with 3 mm Allen key see detail image 5 to left at knife holder recommended angle from 2 to 5 With anti roll guide 4 Lever 6 for the lateral shift Lever 7 for clamping the blade Lever 8 for clamping the base 9 to the dovetail guide in the c
35. essive substances for e Drain the cleaning liquid through the hose after the prescribed reagent time is over and collectitin the waste container 1 Emptying the condensate bottle Check the fill level of the condensate bottle 1 visible in the front panel of the instrument at regular intervals e The bottle collects the condensate that accumulates during defrosting Dispose of the contents of the bottle in accordance with laboratory regulations Fig 67 66 Operating Manual V1 1 05 2007 12 Maintenance and Cleaning Basically we recommend UV disinfection see page 35 for detailed instructions For easy to use spray disinfection we recommend Leica Cryofect Cryofect is not available in all countries The cryostat has to be disinfected after each daily use It is not necessary to oil the parts such as the T piece on the microtome base plate clamping lever etc Comply with the instructions for use The glass anti roll plate can remain in place during disinfection e n case of visible pollution such as dust clean the air inlet opening Fig 10 p 18 of the condenser on the bottom right hand side of the instrument using a brush broom or extraction cleaner in the direction of the louvers Do not turn the instrument on before the cryochamber is completely dry Frost formation The front panel and the slit cover of the microtome must be com pletely dry before turnin
36. g on the instrument Dry all parts completely before reinserting them in the cryochamber 12 2 Changing Fuses e Incase of power supply faults please contact an authorized Leica service technician immediately Do not carry out any repairs on your own as this will invalidate the warranty Repairs may only be carried out by qualified service engineers authorized by Leica Leica CM1950 Cryostat 67 12 Maintenance and Cleaning 12 3 Replacement of the Lamp UVC Lamp Turn the instrument off with the automatic power fuse and pull the power plug before replacing the lamp If the lamp is broken it must be replaced by the technical service as the replacement involves a high risk of injury Beware of the metallic mercury in the UV lamp handle it carefully and dispose of it properly A UVC lamp has an estimated service life of approx 9 000 hours Each on off switching cycle reduces the lamp life by approx one hour plus burning time 30 minutes or 180 minutes respectively When both disinfection indicator lights are blinking alternately in control panel field 1 the UVC lamp must be replaced Sub 248 Instructions on how to replace the UVC lamp are provided together with the replacement lamp spare part 68 Operating Manual V 1 1 05 2007 12 Maintenance and Cleaning Leica CM1950 Cryostat e Slightly lift the sliding window 1 holding it by the grip 2 and pull it
37. hamber Pressure plate 3 for section extraction When using low profile blades the ruler 7 Fig must be inserted Assembling the anti roll guide system for the blade holder CE 1 The glass of the anti roll guide can be used from all 4 sides when replace Insert the glare minimizing glass insert into the interchangeable frame and tighten it evenly using the knurled screw 10 Insert the shaft 11 of the metal frame for exchangeable glass inserts from above into the bore ofthe swinging arm 12 in such a way that the pin rests in the notch Push the white plastic plate 14 from below onto the shaft 11 Screw the knurled nut 13 from below onto the shaft 11 ment is necessary the glass stage plate can be reordered 99 11 Optional Accessories 4 The anti roll guide and pressure plate must be parallel to each other Red elements on the blade or knife holders such as the knife guard and ejector are protective devices that may not be removed e Blade ejector 1 and knife guard 2 e Integrated lateral shift and stable base e Clearance angle adjustment using size 3 Allen key recommended angle between 2 and 5 e With finger rest 6 for brush specimen e Clamping lever 8 for lateral shift must point downward to permit unhindered shifting of finger rest e when using BB knives remove the knife support 7 Conversion of blade holder with anti roll guide Blad
38. he box 7 at the top 2 Remove the 2 screws on each of the 4 hinges 2 on the front and rear side of the box Do NOT remove the screws of the hinges on the top lid 3 Remove 1 screw 3 between the two hinges on the front and rear side of the box 4 Remove the 3 screws 4 at the bottom on all four sides of the box 5 Of the two vertical rows of screws 5 remove one on either side which side does not matter entirely Operating Manual V 1 1 05 2007 2 Safety and Design Removing the packaging Remove the cover 17 and place it on the floor with the foam parts facing downwards The cover will be used as a ramp later Remove the 4 foam parts 6 by pulling them upwards Open the box 7 on its side hinges like an accordion partition and remove It Remove the accessories carton 8 and operating manual on the front of the instrument Pull off the dust cover 9 by pulling upwards Take off the foam strip 10 on the bottom rear After the foam strip 10 is removed 2 screws 12 are visible on the baseplate ewing out the cover hinges 2 atthe front and rear Attach the cover 17 from the rear on the transport pallet 11 The notches 13 ofthe hinges 2 mustbe pointing towards the instrument Move the cover towards the left so that the notches 13 of the hinges slide beneath the heads of the screws 12 Transport to the Installation Location
39. he slow forwards movement to the knife To feed the specimen press and hold the button Pressing the button briefly results in a feed motion of 20 um e Start the fast forwards movement to the knife The LED 2 flashes while the specimen head is in motion The LED 2 lights up when the forward end position has been reached il o E fast Manual sectioning mode Select ROCK operating mode LED active retraction must be enabled e For sectioning turn the handwheel a short distance approx 1 4 turn forwards and back rocking mode only possible at rear handwheel in approx 12 3 o clock position Every change in rotation direction is electronically detected and automati cally translated into a specimen feed or retraction movement Leica CM1950 Cryostat 33 1 Instrument Controls Control panel 3 Motorized sectioning optional Switching the sectioning mode i If emergency stop has been activat CUT MODE from continuous ed the cutting mode must be select stroke CONT to single stroke ed again SINGLE active The red LED in the E STOP field indicates either e emergency stop function active or footswitch dummy optional footswitch connected incorrectly or not at all Motor speed controller 0 100 The mechanical handwheel brake is enabled when the yellow LED is lit in the M STOP field Hold the button to section at maximum speed Release the button to con
40. ht or slightly inclined position e To ensure a safe transport with a fork lift 3 people are required one op erating the fork lift and the other 2 holding the instrument on either side to prevent it from sliding down e Before connecting to the power supply system please check if the local voltage complies with the power rating specified on the name plate of the instrument see also Technical data e Never connect the instrument to a power socket that does not have a protective conductor terminal Length of power cable up to 3 5 m extension possible no i After transporting wait at least 4 hours before turning the instrument on This waiting period is necessary to allow the compressor oil which may have been displaced during transport to return into its original position Any condensation on electrical parts that has formed due to tempera ture differences during transport must be allowed to dry completely Failure to comply with this can cause severe damage to the instrument Leica CM1950 Cryostat 2 Fig 3 Safety and Design When the instrument is delivered check the tilt indicators on the packaging If the arrowhead is blue the shipment was transported laying flat was tilted at too great an angle or fell over during transport Note this on the shipping documents and check the shipment for possible damage i Opening the packaging 1 Remove the 3 screws 1 on each of the two sides of t
41. i roll guide system at a high sec tion thickness e g 10 um Start from there and work your way down to the desired section thickness in small increments readjusting the anti roll guide system at each increment using the knurled nut Fig 48 Knife holder CN with glass anti roll guide 58 Operating Manual V 1 1 05 2007 H Fig 49 11 Optional Accessories Cleaning the knife holder CE Daily cleaning 1 Fold the anti roll guide system 33 to the left while holding it down by the lever 34 2 Unscrew the bolts 35 of the pressure plate 3 Afterwards the pressure plate 36 can be re moved for cleaning with alcohol or acetone For disinfection commercially avail able mild cleaners and disinfectants can be used we recommend Leica Cry ofect Cleaning the knife holder CN Daily cleaning i Fig 50 Disinfection e g with Leica Cryofect For daily cleaning it is enough to fold the anti roll guide system forwards and remove the section waste from the knife holder using a dry brush Please use a cold brush as otherwise the section waste will thaw and stick to the knife holder It is not necessary to oil the parts such as the T piece on the microtome base plate clamping lever etc Spray contaminated surfaces with a uniform coat of concentrate or wipe them down with a rag soaked in it allow it to make contact for 15 minutes then wipe tt off Leica CM1
42. imen temperature is set and indicated on the control panel marked with the specimen head symbol The actual temperature is the standard indication Briefly pressing the or button displays the target temperature Set the desired value via the buttons Pushing the or button for more than 1 sec increases or decreases the specimen temperature continuously The actual value will be indicated 5 seconds after finishing the program ming Caution The specimen head and Peltier do not switch on until the chamber temperature reaches 5 C in order to prevent icing Specimen temperature Max Cool function e The snowflake button for the Max Cool function is in the panel with the specimen head symbol Pressing the button sets the lowest possible specimen head tem perature 50 C as the target temperature The instrument adjusts the maximum low temperature of the specimen head i e 50 C e Push the snowflake button again to stop the Max Cool function The temperature adjusts to the value programmed prior to activating the Max Cool function e Alternate flashing of LL and the actual temperature indicates activa tion of the Max Cool function Setting the time e The clock time is set with the buttons in the control panel marked with the clock symbol To do so set the current time using the or button below the small clock symbol Pushing the or button for more than 1 sec increases or d
43. l eee 0201 08926 1 pair of safety gloves size M for cryosectioning es 0340 29011 1 operating instructions plus language CD sees 0708 37115 Note for the Japanese version 100V 50 60 Hz 1 pair of safety gloves size S 0340 40859 is included Basic instrument WITH and WITH extraction in the specific voltage variant standard scope of delivery as above plus 1 accessory set extraction anm dct RE reb br bee rar 0477 43300 gt Hose adapter vr eb imn ce fun Ctr d noir 0477 40293 Hose adapter AE T TTE 0477 40294 set OP eee men ne nme eet Se ene 0477 40295 NNN 0477 43302 Silicone TN 0477 43304 Chamber suction nozzle Lio otio rac t tee er es 0477 43719 Set of filters b pieces sssssseennnnnnnnnnnnnnnnnnnn 0477 43792 Compare the delivered components with the parts list and your order Should you find any discrepancies please contact your Leica sales office without delay i A choice of different knife holders is available for the CM1950 Leica CM1950 Cryostat 17 5 General Overview Emergency stop On off switch also circuit switch motorized breaker instruments only 290000 amp jl fae Footswitch dummy motorized instruments only Cryostat chamber with activated UV disinfection Handwheel in 12 o clock position Bacterial filter optional only for instru
44. lter 3 into the ex traction hose connecting piece 3a until there Is an audible click Now push the connected parts back into the opening in the cryostat chamber filter first and set it to the closed mark 2 Fig 27 When not using the extraction tightly close the opening for the extraction hose using the silicone stopper E in cluded in the scope of delivery Reasons 1 2 To prevent section waste from falling into the opening To prevent cold from escaping from the chamber To prevent moisture from penetrating into the chamber Operating Manual V 1 1 05 2007 6 Installation 6 5 9 Installing the Section Extraction Optional Use with Blade Holder CE Only e Silicone hose A with hose adapter 1 B to the filter in the instrument hose adapter 2 C for suction nozzle D or F and suction nozzle D factory pre assembled e Silicone stopper E e Chamber suction nozzle F e Filter G e Plastic clip H for parking the chamber suction nozzle Mark for suction nozzle When the suction nozzles are changed the adapt er white remains in the silicone hose Pull off the nozzle by rotating and pulling it gently and firmly plug in the desired nozzle i The tension acting on the hose can be minimized by turning the red ring Fig 31 top right clock wise so that the suction nozzle presses against the pressure plate I Fig 31 Afterwards fold the anti
45. m Values 1 0um 50um 1n0 5um steps Range of trim thickness 1 600 um 50pm 20 0um in 1 0 um steps Recommended for research applications 20 0um 60 0um 1n 5 0 um steps Values 1 0 um 10 0 um in 1 0 um steps 60 0um 100 0 um in 10 0 um steps 10 0um 20 0um in 2 0 um steps 20 0um 50 0um in5 0 um steps 50 0 um 100 0 um in 10 0 um steps 100 0 um 600 0 um in 50 0 um steps Trimming section thickness setting range Recommended for clinical applications Values 10 um 20 um 30 um 40 um 32 Operating Manual V 1 1 05 2007 7 Instrument Controls Coarse feed functions The two speed electric coarse feed is designed to quickly position the spec imen in relation to the knife The buttons with double arrows move the spec imen at 900 um s the buttons with single arrows move it at 300 m s Sle Je Retracting the specimen head from the knife e Pressing once starts the rapid backwards movement to the rear end position Home position The LED 1 flashes while the specimen head is in motion The LED 1 lights up when the rear end position Hp has been reached e Theretraction can be stopped by pressing one ofthe coarse drive buttons e The rapid backwards movement to the rear end position Hp starts The motion continues as long as the button is held brief press of the button retracts the specimen by 20 um S 2 KS amh gt 5 o Advancing the specimen toward the knife e Startt
46. m and 100 pm anti roll plates are included in the standard scope of delivery of the blade holder CE Straightedge blade rest Insert for low profile blades for blade holder CE 0477 43005 replacement The blade rest is also included in the standard scope of delivery of the blade holder CE Bacterial filter e Bactericidal filter 350 5865 pack of 1 unit Rec ommendation replace bactericidal filter every three months note date on filter when installing it Filter bags and bacteria filter must be disposed of according to applicable laboratory regulations for infectious material Filters must be replaced not cleaned Operating Manual V 1 1 05 2007 11 Optional Accessories Replacement filters for extraction system pack of 25 e Change when vacuum power Is Impaired e When using the cryostatfrequently check the filter bag daily and replace as required Fig 57 Vacuum extraction system A Hose B Hose adapter black for the filter bag in the instrument C Hose adapter white for suction nozzle D or extraction nozzle F D Suction nozzle with 4 magnets E on knife holder G Plastic clip for parking the suction nozzle Storage system complete hidden e forinstallation in rear area of cryostat for cold storage of specimen discs and sectioning ac cessories For assembly refer to page 26 Fig 59 Leica CM1950 Cryo
47. men disc specimen detached from disc after freezing Remedies Use different part of the knife Replace anti roll plate Refreeze specimen onto the disc Check disc clamping Check knife clamping Refreeze specimen onto the disc Increase section thickness reduce specimen surface area if ecessary Use different part of the knife Use knife with different profile set correct angle store all tools on storage shelf in the cryochamber Replace anti roll plate Be more careful next time select correct temperature Use knife with different profile c or d Remove Ice Adapt speed Check knife clamping Check disc clamping Apply cryocompound to warm disc mount specimen and freeze 49 9 Troubleshooting Problem Tissue sticks to the anti roll plate Flattened sections curl up when anti roll plate is folded up Sections tear Cryostat non operational Specimen disc cannot be removed 90 Causes Blunt knife Inappropriate section thickness Incorrect clearance angle Microtome not dried thoroughly enough Dried specimen Anti roll plate is too warm or incorrectly positioned Fat on the corner or edge of anti roll plate Anti roll plate not correctly fixed Rust on the knife Anti roll plate too warm Temperature too low for the tissue cut Blunt part dirt dust frost or rust on the knife Top edge of the anti roll plate damaged Hard particles in the tissue
48. ments with filter Unscrew and align adjusting feet after transport Catch tank for condensate E O ihi Wh Uo Castors for safe transport __ over short distances Align adjusting feet for secure upright position 18 Operating Manual V 1 1 05 2007 5 General Overview 5 1 Control Panel Fields and Cryostat Chamber 1 Control panel field 1 Extraction temperature and time control illumination UV disinfection 2 Control panel field 2 Electric coarse feed sectioning and trimming thickness adjustment 3 Control panel field 3 Motorized sectioning cutting optional adjustment of stroke type cutting speed etc alli uge eae E Ag Protect OG 2 Fig 11 4 Heat conductor block stationary optional 10c Knife guard on the blade holder CE 5 Peltier element with 2 stations 11 Extraction nozzle on the extraction hose 6 Freeze shelf 15 positions 12 Extraction hose for section waste 7 Position holder on freeze shelf 13 Brush shelf optional 8 Heatand cold extractor mobile opt 14 Adapter piece for extraction hose 9 Shelf movable optional the coarse filter insert is behind it 10 Blade holder CE with blade ejector a 15 Object head directional 10b Finger rest on the blade holder CE 16 Waste tray Leica CM1950 Cryostat 19 6 Installation 6 1 Site Requirements The place of installation must meet the following requiremen
49. ming to sectioning mode important for section stretch ing as the valves work differently than in trimming mode set the desired section thickness Switch on the VAC and begin at level 1 If the section does not stretch correctly turn the VAC knob higher in small increments Once the desired section is on the pressure plate switch off the VAC Carefully fold the anti roll guide to the side and remove the section from the side After removing the section wipe off moisture condensate from the pressure plate otherwise the next sections will become jammed Cutting with extraction without anti roll guide brush technique is not possible as the position of the pressure plate means that no suitable air flow is obtained 45 8 Working with the Instrument few rules e Always begin at a low extraction level then slowly increase It e Donotuse high extraction levels unless absolutely necessary e Different specimen sizes require different extraction levels e he faster the trimming or sectioning speed the lower the extraction level should be e he larger and or thicker the specimen to be trimmed the lower the extraction value e When section specimens with a diameter of 0 5 cm the anti roll guide stretches the section adequately For larger specimens we recommend using the vacuum function Trimming 1 x 1cm specimen Trimming 1 x 1cm specimen Trimming thickness Speed Extract value Trimming thickness Speed Ex
50. n alcohol based disinfectant or remove the waste using the extraction nozzle optional Do not start the disinfection until after folding the section stretcher to the side Each new specimen is a potential source of contamination e When disinfecting the instrument take appropriate protective measures gloves mask protective clothing etc e When using detergents and disinfectants please comply with the safety precautions of the disinfectant manufacturer e The integrated glass anti roll guide of the blade holder CE CN and CN Z can be cleaned either with acetone or alcohol e Dispose of waste liquid according to the waste disposal regulations e Donotuse external heaters for drying the cryochamber This can cause damage to the cooling system e Donotturn the instrument on before the cryochamber is completely dry Frost formation e All components removed from the cryostat must be carefully dried be fore returning them to the cryochamber e The front panel and the slit cover of the microtome must be completely dry before turning on the instrument Microsystems website at i For more detailed information about disinfection visit the Leica www histo solutions com 12 Operating Manual V 1 1 05 2007 2 Safety and Design 26 Handling Specimens Defrosting e When working with contaminated or infected material the general safety guidelines for laboratories must be applied e Before defrosting the cryoch
51. ns to buttons to buttons to Melting UVC enable disable enable disable select select select real Snowflake disinfection vacuum the Peltier chamber specimen time button to short time 30 min extractor element temperature head activate manual long time 180 min temperature buttons to defrost Extraction select defrost force intensity time selectable Max Cool button to ON OFF button of select the maximum low temperature of the specimen head directly 50 C from 1 5 see illumination following page for more information Press the key button to lock unlock the entire keypad Refer to following page for activation of the specimen head EMERGENCY STOP switch to the right of control panel field 1 motorized instruments only For danger situations during motorized sectioning e Immediate stop of the sectioning process motor stops LED in E Stop Is illuminated in red e Turning in direction of arrow cancels the stop LED in E Stop goes out e Select single stroke Single or continuous stroke Cont operating mode again 30 Operating Manual V 1 1 05 2007 7 Instrument Controls 6 e Press the VAC button to enable the vacuum extractor The LED in the o VAC button is lit while the extractor is on Press the button again to NUPI disable it Position e Use the knob to adjust the intensity of the vacuum 6 o clock A Optimal area for trimming and sectioning e Trimming Handwheel p
52. nscrew the adjustable feet using the No 13 open end wrench when subse quently transporting the instrument on cas tors screw the feet back in as far as they will go To ensure a secure upright position at its intended location align both adjusting feet 1 Before transport or relocation remove the filter bag from the chamber If you fail to do this the filter bag will thaw then freeze solid when the instrument is reconnected When subsequently removed the filter will be destroyed causing section waste to get into the bacteria filter Refer to p 26 When not using the extraction for a long period tightly close the opening for the extraction hose using the silicone stop per 5 page 28 included in the scope of delivery 2 covered Leica CM1950 Cryostat 21 6 Installation Transport with a fork lift e The instrument can be transported with a fork lift To ensure a safe transport with a fork lift 3 people are required one operating the fork lift and the other 2 holding the instrument on either side to prevent it from sliding down Atthe installation site unscrew the adjustable feet 1 with the open end wrench No 13 see Fig 12 This is absolutely necessary for a stable stand Total width with handwheel Fig 13 63 Assembling the Handwheel Rotate the handwheel only if the refrigeration system is on and the cryochamber is cold
53. o use another part of the blade To do so follow these steps 1 Release the clamping lever 8 by folding it back and move the knife holder back until the desired position is reached 3 point click stop enables you to accurately find a new cutting position 2 Fold the clamping lever 8 forwards for clamping 57 11 Optional Accessories Adjusting the Knife Holder with Anti roll Guide You can adjust the height of the anti roll guide system using the knurled nut 1 e fyouturn the nut counterclockwise the anti roll guide system moves toward the blade e fyouturnthe nut clockwise the anti roll guide system moves away from the blade If the anti roll guide system Is in the wrong posi tion relative to the cutter the following problems will result Blade holder CE with glass anti roll guide Fig 47 Fig I The section rolls over the glass insert of the anti roll guide system Error Glass insert not high enough Remedy Turn the knurled nut counterclockwise until the section is pushed between the blade and anti roll guide as shown in Fig III Fig Il Section tears and block hits the glass insert after sectioning The section rolls over the glass insert of the anti roll guide system Error Anti roll guide system is set too high Remedy Turn the knurled nut clockwise until the section is pushed between the blade I and anti roll guide as shown in Fig Ill Generally we recommend pre adjusting the ant
54. old in Japan 50Hz 60Hz Selecting the frequency e After unpacking the instrument and setting it up at its intended location use the lever 1 to select the frequency corresponding to the conditions of the existing power system Power plug 24 Operating Manual V 1 1 05 2007 6 Installation 65 Installing Accessories Inserting Chamber Accessories 6 5 1 Installing the Adjustable Footrest Optional o e To install the optional footrest unscrew the screws 1 using the No 3 Allen key provided When installing the holder 2 ensure that the cutout faces downwards so that the support 3 can be hooked in e Attach the holders 2 for the footrest on the left and right to the front wall of the housing To do so use the Allen screws you used earli er Ensure that the screws are tight e Hook the footrest 3 into the installed holder according to individual requirements height e Once installed the user can adjust the height of the footrest at any time by relocating it 3 to the desired height on both sides in the holder 2 Fig 20 Leica CM1950 Cryostat 25 6 Installation 652 Installing the Storage Systems Optional For reasons of tro EEE accessibility the MHP TTT optional storage system must always be installed first To do so remove the insert 1 place the frame 2 in front of the bore and tighten the screws washers on the cryostat housing
55. op erly closed Closing the window activates the corresponding safety features The consistent use of these safety features and strict observation of the warn ings and cautions in this manual will safeguard the operator from accidents and or personal injury to a great extent Microtome knives 10 e Take care when handling microtome knives disposable blades The cutting edge is extremely sharp and can cause severe injury e Never leave knives and dismounted knife holders with a knife blade mounted lying around e Do not place a knife on a table with the cutting edge facing upwards We strongly recommend using the safety gloves included with the standard delivery e Never try to catch a falling knife e Prior to manipulating the knife and specimen or changing the specimen always lock the handwheel and cover the cutting edge with the knife guard e Avoid contact with cold parts of the instrument as this can cause frostbite Operating Manual V 1 1 05 2007 2 Safety and Design Knife guard Prior to manipulating the knife and specimen changing the speci men or knife or taking a break always lock the handwheel and cover the cutting edge with the knife guard The CE CN and CN Z knife holders feature knife guards the glass anti roll plate of the CE knife holder also serves as a knife guard 24 Securing Locking the Handwheel Always lock the handwheel and cover the cutting edge with the 12 o clock p
56. osition knife guard prior to manipulating the knife or specimen changing the specimen or taking a break To lock the handwheel press the lever 1 outward Continue turning the 6 o clock handwheel slowly until the grip is in the upper or lower position and the position handwheel is locked Press the lever fully outward gently rock the hand Fig 7 wheel until the locking mechanism clicks into place noticeably To release the handwheel press the lever 2 on the handwheel toward the cryostat housing Centering of the handwheel motorized instruments only Pull out the handwheel s handle and position it in the middle of the handwheel The handle automatically engages in this position i An important safety device on the cryostat is the centering of the handwheel for motorized instruments Rotate the handwheel only if the refrigeration system is on and the cryochamber is cold Leica CM1950 Cryostat 11 2 Safety and Design 25 Cleaning Disinfection Turning the Instrument Back On i It is not necessary to remove the microtome for disinfection e The instrument has been designed for UV disinfection Spray disinfec tion with Leica Cryofectis also possible thanks to the special insulation of the microtome Cryofect is not available in all countries Remove section waste after EVERY sectioning operation and BE FORE changing specimens Remove the section waste using a pa per towel soaked in Cryofect or a
57. osition 12 6 o clock valve open Handwheel position 6 12 o clock valve closed e Cutting Handwheel position 12 3 o clock valve open all the way Handwheel position 3 6 o clock valve half open Handwheel position 6 12 o clock valve closed B Optimal area for extraction from the chamber e To clean the chamber turn the knob to the red range i The strength of the required extraction force depends on the following e Size of the specimen e Sectioning speed e Section thickness used e The Peltier element provides additional cooling for the freezing stations g PE After the e button is pressed the display changes from PE to 10 indicating an additional cooling period of 10 minutes The remaining cool ing time is always shown in the display Once only 4 minutes are remain ing a dot will appear after the 4 As of this time the Peltier element may be switched off early by pressing the e button again Caution The specimen head and Peltier do not switch on until the chamber temperature reaches 5 C in order to prevent icing If the condenser resting phase is off and the Peltier is activated the digit 10 flashes until the condenser switches on again to pre vent the Peltier from being destroyed when the condenser is not running When the condenser starts up the flashing starts and the 10 minutes are counted down m For exact instructions for using the chamber specim
58. out to the front Removal of the lamp e Thelamp is mounted behind a glare shield and therefore not visible e Touch the lamp for better orientation e Lightly tilt the fluorescent tube down to the left and pull it out of the clip 3 e Hold the lamp with both hands and pull it to the left out of the holder 4 Only use lamps of the same specifica tion Installation of the new lamp Type OSRAM DULUX L 18W 840 e Hold the lamp in the correct mounting posi tion as shown and push it to the left until it engages in the holder 69 13 Decontamination Certificate master Dear Customer Any product which is to be returned to Leica Microsystems or serviced on site must be cleaned and decontaminated in the appropriate manner Leica accepts that itis not possible to decontami nate for CUD and its variants and in this instance following current customer safety guidelines Is acceptable Equipment exposed to CJD samples cannot be returned to Leica Microsystems for repair This certificate duly completed must be placed in the unit prior to packaging or handed over to the service engineer Packages will not be opened nor servicing commenced until the Company or service engineer have received a satisfactory certificate Should returned goods be considered a hazard by the Company they will be returned immediately to the customer at his her expense NB Microtome knives must be in boxes Description Name Model Fabr No KA
59. panel for the specimen cooling Ten seconds after the manual defrost cycle has been completed the specimen cooling turns back on Before starting the defrost cycle remove all specimens from the cryochamber Manual defrosting of the specimen cooling 40 Push the manual defrost button with the melting snowflake on the left over the key button to activate the defrost cycle of the specimen head Activation is confirmed by an audible signal Then push the or button on the panel for the specimen temperature There is a flashing indication of the specimen temperature during the defrost cycle For 15 minutes the specimen head is adjusted to a temperature of 45 C Subsequently the instrument adjusts to the specimen temperature which has been programmed prior to the manual defrost cycle If necessary push the manual defrost button again to deactivate the manual defrost cycle Press the key button then the button specimen head on Press the key button then the button specimen head off Operating Manual V 1 1 05 2007 8 Working with the Instrument Leica CM1950 Cryostat Entering the trimming thickness i To switch from a trimming section thickness for research applica tions 1 600 pm to a thickness for clinical applications 10 20 30 or 40 pm press and hold down the TRIM SECT key while switching on the instrument e Press the TRIM SECT button TRIM mode is active if the LED a
60. protected by the knife guard in this 60 Operating Manual V 1 1 05 2007 11 Optional Accessories Fig 52 Fig 53 Knife guard lateral movement for knife holder CN The knife guard 18 is fixed and integrated into the clamping jaws The knife guard has handles 19 that allow it to be moved The knife quard is adequate for knives up to 22 cm in length Always cover exposed parts of the knife blade after sectioning The anti roll guide system can be moved side ways only for the 84 mm variant To better find the mid position a groove 17 is provided in the shaft 16 Knife holder CNZ with anti roll guide e Pressure plate permits the full length of the knife to be utilized e Use hard metal and steel knives here The height of resharpened knives must be adjusted using knurled screws 3 approx 1 mm under the edge of the clamping jaw Ensure that the knife is adjusted in parallel vertically Leica CM1950 Cryostat 61 11 Optional accessories Fig 55 When using low profile blades the blade rest 7 must be inserted into the blade holder first then the low profile blade see page 50 Fig 40 62 Anti roll quide system Anti roll plate with glass stage plate Available with various spacers e 70mm 50um for section thickness lt 20 um 70mm 100 um for section thickness 20 um 100 um e 70mm 150 um for section thickness gt 100 um The 50 p
61. roll guide K back onto the pressure plate Ensure that the hose with the nozzle is not installed against its natural cur vature on the pressure plate of the blade holder e he scope of delivery also includes 2 plastic clips H These enable comfortable parking of the chamber suction nozzle F during sec tioning The clip mustbe glued in before switching on the refrigeration Before doing so briefly degrease the surface to ensure a secure hold Preferably the clip should be attached outside the working area e g on the left inside wall of the instrument H If the extraction nozzle D is not being used it can be parked on one of the two magnetic surfaces indicated in the interior of the instrument If the extraction is not used for a long time it is absolutely necessary to clean the extraction hose in order to ensure maximum extraction capacity To do so place the hose in commercially available disinfectant or alcohol After several cleanings the hose must be replaced see order information on p 49 Leica CM1950 Cryostat 29 7 Instrument Controls 11 Control Panel Fields on the CM1950 Control Panel Field 1 Display of actual and target chamber Display of actual and target specimen Display of real time defrost time temperatures head temperatures and error messages 11 i aa menn MA EEE et Button to Button to butto
62. stat 63 11 Optional Accessories e Specimen discs in various dimensions The specimen disc 50x80mm is suitable only for section thicknesses up to approx Ld 5um due to the large specimen size g 40 930 o 20mm O rings in various colors m bis immi Mel e for plate 20mm red or blue 10x each va sot 49 vari Pisctam a oe e for plate 30mm red or blue 10x each e for plate 40mm red or blue 10x each e for plate 55mm red or blue 10x each for color marking of pecimen disc A Heatextractor with parking station stationary e for installation in the cryostat consisting of support device heat extractor and parking sta tion B Heatand cold extractor e suitable for two different applications extracts cold when stored in warm environment ex tracts heat when stored in cold environment e The specimen discs of the Dr Peters cooling m es Y Dr Peters i specimen discs system can be conveniently removed fromthe Shelves A I shelf using the underside of the heat extrac e EUG z i tor removal aid D Slide the underside over ae the specimen disc in the direction of the ar ed 3 AR EELLL row so that the disc remains in the slot and ECCE can be removed from the rack td i wae TE Embedding well bar large with 3 wells LxWxH 30 x 30 x 7 mm 5 EE Embedding well bar medium with 4 wells LxWxH 24 x 24 x 6 mm Embedding well bar small with 4 wells LXWxH
63. t the top right is illuminated e Setthe desired trimming thickness using the or button in control panel field 2 Refer to page 32 for adjustable sequence of steps Entering the section thickness e Press the TRIM SECT button SECT mode is active if the LED at the bot tom right is illuminated e Set the desired section thickness using the or button in control panel field 2 Refer to page 32 for adjustable sequence of steps Switching the retraction on or off in manual sectioning mode e Press the TRIM SECT button for approx 3 sec The LED in control panel field 2 displays on or OFF Pere I i 1 e You can switch by pressing the or button e Retraction on means a specimen retraction of 20 um in manual mode 41 8 Working with the Instrument 8 4 Working with the Precooled Cryostat 8 4 1 Preparatory Work e Lock the handwheel in the top 12 o clock position e Cutthe specimen to size outside of the cryostat e Select the precooled specimen disc cover it with freezing compound then attach and orient the specimen e Attach the specimen disc and specimen to the Peltier position on the freeze shelf Activate the Peltier element and wait until the specimen is completely frozen Specimens that have been frozen on the Peltier element are often too cold and split apart during sectioning Allow time for the speci mens to become acclimated e Insert the specimen disc into the specimen hea
64. ternton iea 22 Unpacking and Installation Lunnsmeme resnanainnsnemndnedkGsendnsdarudnendsGjing 1 23 oale Fedre NOT 10 2 4 Securing Locking the Handwheel eene 11 25 Cleaning Disinfection Turning the Instrument Back On sss 12 26 Handling Specimens Defrosting seen 13 2 7 FAGENE 13 LG GAL 1 EEE EE 13 Technical Data m 14 Standard Delivery f m 16 General Overview sese teen 18 5 1 Control Panel Fields and Cryostat Chamber sene 19 FELE ET EEE eee E see eee eesti 20 TEMERE 20 ETNE 21 6 3 Assembling the Handwheel Centering the Handwheel Optional 22 6 3 1 Locking Unlocking the Handwheel eenennnne 23 6 3 2 Installing the Footswitch Dummy Instruments with Cutting Motor 23 6 4 Connection to Power Supply System rnnserervenrenerverrerveververveserverversavssvesverssnesversessenesvervenen 24 65 Installing Accessories Inserting Chamber Accessories eene 25 6 5 1 Installing the Adjustable Footrest Optional assesseer 25 6 5 2 Installing the Storage Systems Optional enn 26 6 5 4 Inserting the Section Waste Tray NG 26 6 5 3 Shelf Movable Optional sssssssessssrsrsrssrsrsrssnsrsrnun
65. tes or deactivates the manual defrost of the cooling chamber specimen head or freeze shelf For more detailed handling instructions refer to the chapter on Working with the Instrument on p 39 of this Operating Manual Pressing the key button for approx 5 sec locks the entire keyboard the LEDs in the clock go out Pressing the key button briefly then the key in the specimen head control panel field switches off the specimen head Pressing the key button briefly then the key in the specimen head control panel field switches the specimen head back on Programming the temperature of the cryochamber The temperature of the cryochamber is set and indicated on the control panel marked with the cryostat symbol The actual temperature is the standard indication Briefly pressing the or button displays the target temperature Set the desired value via the buttons Pushing the or button for more than 1 sec increases or decreases the chamber temperature continuously The actual value will be indicated b seconds after finishing the program ming values given there are based on long term experience but are i Refer to page 52 for a table with guide values The temperature intended solely as guide values as any tissue may require particu lar adjustments 37 8 Working with the Instrument Programming the specimen temperature e Selectthe desired temperature of the specimen e he spec
66. the or button on the panel for the specimen cooling and then the key button e The temperature of the specimen cooling first raises to 10 C and then adjusts to the programmed specimen temperature Manual defrost for the freeze shelf including Peltier element 7 e Press the key for manual defrost 8 then the e key ey The manual defrost takes 12 min e Push the button and then the e key again to stop the manual defrost After defrosting the freeze shelf carefully wipe it out as otherwise a lot of water collects in the channel Ice does not melt during normal defrosting Leica CM1950 Cryostat 39 8 Working with the Instrument Manual defrosting of the cryochamber Push the manual defrost button with the melting snowflake on the left over the key button to activate the defrost cycle of the cryochamber on demand Activation is confirmed by an audible signal Then push the or button on the panel for the cryochamber temperature The manual defrost cycle 12 min is activated There is a flashing indication of the temperature of the cryochamber during the whole defrost cycle If necessary push the manual defrost button again to deactivate the manual defrost cycle When the manual defrost begins the specimen head adjusts to a tem perature between 10 C and 5 C reduction of ice formation The specimen head cooling turns off This is confirmed by the flashing of the decimal points on the
67. the unit see page 28 change it at least every 3 months e Move the anti roll guide to the side e Spray the cryostat chamber using Cryofect e Activate the UVC disinfection 4 9 Troubleshooting 9 1 Problems During Work Problem Frost on chamber walls and microtome Sections smear sections splinter sections not properly flattened sections not properly flattened despite correct temperature and correctly aligned anti roll plate Sections curl on anti roll plate Scraping noise during sectioning and specimen return movement 48 Causes Cryostat is exposed to air currents open windows and doors air conditioning Frost built up by breathing into the cryochamber Specimen not cold enough Anti roll plate not yet cold enough thus warming the section Specimen too cold Static electricity air currents Specimen not cold enough Large area specimen Anti roll plate poorly positioned Anti roll plate poorly aligned with knife edge Incorrect clearance angle Blunt knife Dirt on knife and or anti roll plate Edge of anti roll plate damaged Blunt knife Anti roll plate does not protrude far enough beyond the knife edge Anti roll plate protrudes too far beyond the knife edge and is scraping against the specimen Remedies Change place of installation for the cryostat Wear mouth protection select lower temperature Wait until knife and or anti roll plate have reached
68. tinue section ing at the previously selected speed see controller above AN The handwheel must also be locked when working on the specimen head Press to brake the handwheel electronically LED lit specimen stops in lower 6 o clock position Switch from slow speed Can he used in any position range to fast speed range while switching on the in e Press the buttons at the same time to start motorized sectioning 2 To end the sectioning process press RUN STOP ENABLE or BRAKE strument press and hold down the Vmax button Fig 34 Specimen head stops at bottom for BRAKE automatic electronic braking takes place Speed ranges 3 For motorized sectioning does not have to be Slow 0 50 strokes min unlocked continue working by pressing both Fast 0 85 strokes min the RUN STOP and ENABLE keys Vmax 85 90 strokes min 4 If during manual work using handwheel you have Sectioning modes braked using BRAKE also use BRAKE to release The instrument can be used in manual and motorized modes The following settings are available e Single stroke SINGLE or continuous stroke CONT in automatic mode as well as e ROCK sectioning using handwheel in manual mode i When switching the instrument on no operating mode is active for safety reasons 34 Operating Manual V 1 1 05 2007 Disinfection Duration 30min Duration 180 min 7 Instrument Controls To start disinfection
69. tract value 10 um 40 1 5 1 um 5 um 40 2 5 10 um 60 S 60 2 10 um 80 1 80 1 5 10 um 100 1 100 1 5 20 um 40 2 10 um 40 25 20 um 60 2 60 2 20 um 80 15 80 2 20 um 100 1 100 1 5 i The values listed here are possible settings that depend on the tissue type specimen size section thick ness and section speed and are in tended for orientation purposes only These settings can also vary when using different anti roll plate 46 Operating Manual V 1 1 05 2007 8 Working with the Instrument After trimming or sectioning Leica CM1950 Cryostat Specimen e Unclamp and thaw e mmerse in fixative for further processing Cleaning e Use the brush to sweep up the section waste section waste tray and remove itfrom the cryostat follow applicable laboratory regulations for disposal Or e Clean the cryostat chamber using the chamber suction nozzle e To do so turn the flat suction nozzle of the extraction hose by holding the hose on the white adapter and removing it with a quick twist Park the flat suction nozzle in a designated place in the chamber e g on the right inside wall of the cryogenic chamber e Remove the chamber suction nozzle from the plastic clip and attach it firmly to the white adapter e Check the remaining capacity of the filter in the interior of the chamber and change the filter if necessary see page 28 e Check the bacterial filter in the front of
70. ts e Nodirect sunlight e Power supply at a distance no greater than approx 3 m e No drafts air condition outlets etc e Even floor e Vibration free floor e The instrument is designed for indoor use only e Obstruction free access to the handwheel e The power switch circuit breaker must be freely and easily accessible e Room temperature always approx 22 C e The relative humidity must not exceed 60 e Distance to walls and furniture calculated from the cabinet rear 15cm right side 30 cm left side 15cm e No heat dissipating appliances around High room temperatures and excessive air humidity affect the cooling capacity of the cryostat and lead to ice forming in the instrument e Plug the instrument into power sockets with ground only Power cable length up to 3 5 m Extendable no 20 Operating Manual V 1 1 05 2007 6 Installation 62 Transportto the Site e First check if the location meets the conditions specified in Site requirements e Transportthe instrument to the desired location e Observe the following The instrument must be transported in an upright position or slight ly tilted max 30 When tilting the instrument 2 people must counterbalance from the front side to prevent the instrument from falling down and causing severe injury e When transporting the instrument on wheels 2 grip the cabinet only at the marked loca tions C e To do so u
71. unsnsununurnununnnnrnnnnnnnnnnnnninnnnnnrnrsrnnrnrsrrinnrnrns 26 6 5 5 Installing the Heat Conductor Block Stationary Optional 21 65 6 Installing the Knife Blade Holder and Adjusting the Clearance Angle 21 Operating Manual V 1 1 05 2007 10 11 12 13 14 15 16 Instrument Controls 0 c ccccccccccccccccsceccecccsceccecsssscessscsecsecserserseuseuseusauseusausauseusausansassavussarsuseneess 30 7 1 Control Panel Fields on the CM1950 Control Panel Field 1 30 Control Panel Field 2 Electric Coarse Feed Sectioning and Trimming Thickness Display seen 32 Control panel 3 Motorized sectioning Optional e 34 Working with the Instrument 5 5 seen 36 0 1 Preparing Cutting Tools Specimen Discs and Preparation Aids 36 0 2 Turning the Instrument O Aiaseisinszecvoinsiceninncssntutetndensananapaainterntutsetuntinsgeatanastaset tagennsbneinatsaspeants 36 8 3 Configuring the Parameters susci udmriexisni xingikintia e rimea bkexPE pr ri nir moenia 37 8 4 Working with the Precooled Cryostat rsrrsavrrervenrenvnvervenveververvesveververvenrenervesvesesververveseenen 42 RE La LE EEE EEE 42 0 4 2 Trimming with Extraction Anti roll Guide Installed oseese 43 0 4 3 Cutting
72. using the No 4 Sa Allen key Afterwards insert the insert 1 into the Fig 21 frame and fold it up 6 5 3 Shelf Movable Optional Attach the rod for the shelf to the inner front side of the cryostat housing using the provided screws 1 and the No 3 Allen key then attach the caps 3 The rear side of the movable shelf has white plastic screws 2 that prevent the interior of the chamber from being scratched Now hook the movable shelf into the guide rod Fig 22 6 5 4 Inserting the Section Waste Tray L Before installing the knife Up blade holder base insert the pu section waste tray with the cutout a facing the user 26 Operating Manual V 1 1 05 2007 6 Installation 6 5 5 the Heat Conductor Block Stationary Optional The holder 1 Fig 24 ofthe heat conductor block Is screwed to the left housing wall using the No 4 Allen key provided Preferably begin with the bottom screw Then rotate the holder upwards see arrow and insert and tighten the top screw Fig 24 Now attach the cover for the quick freeze shelf to protect the shelf from 6 5 6 Installing the Knife Blade Holder frost and Adjusting the Clearance Angle For temperature reasons install the knife blade holder on an appropriate base Setthe knife or blade holder 1 Fig 25 on the base 2 adjust the clearance angle on the left of the knife blade holder to approx 2 5 an
73. y 0477 42363 Anti roll plate assembly glass 50MM esse 0419 33901 Heat and cold extractor assembly ees 0477 41039 Cold extractor assembly usessaictrncnenecusteskhn vci aca us vitra iron 0477 43731 Heat extractor assembly eene 0477 43126 53 11 94 Optional Accessories Section waste tray sueisiivitieirit nitrate ki abu idi rU Rad elsi S 0477 40062 IE STIS I EOS 0477 43036 Storage system assembly ssseennnnnnnne 0477 42618 Shel MOVaDIG EE HORN LINE CERES 0477 43037 Retaining EE 0477 40090 Cover freezing shelf cssscucsrseustsccku nrbt RE UV re ek ERU aT ERES UY FE GI EEUU 0477 43763 Specimen dise 3ZX37 DITE cases residere ttis bien kb neo oe 0477 42603 Specimen disc Z29X2B rfl EE 0477 42604 Dar s RITTER 0477 42600 PN 0477 42601 PT veden 0477 42602 Heat extractor Dr Peters assembly sesser 0477 41338 Freezing griddle block elevator ees 0201 39119 DISPENSING slides 8 pleces enne 0201 39127 Foot rest assembly EEE 0477 42832 Accessory kit extraction esee 0477 43300 Bactericidal filter 350 5865 rserrerverevvrverrerverervervesvrvervesvsvesvervessenns 0477 40296 Hose set silicon 5 PlECes m nmmnmnvnvmnnineimeneerien 0477 44469 Filter assembly 25 pieces cccccecsessscsssssencseceesrssesses

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