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HPLC Pump P 6.1L User Manual

1. Flush the pump head before exchanging it Device defect Residuals chemicals can damage the pump head if they are not being removed before storage For reuse flush the pump head and fill it with isopropanol Before storage seal the inlets and outlets with cap fittings Component defect Damage to the pistons if they tilt Undo or tighten diagonally opposite screws evenly by one turn each at a time i i Ls A Loosen the capillary connectors at the outlet Remove the cap illary Fixate the outlet fitting with an open end wrench size 13 Loosen the fitting with an open end wrench size 1 4 Fig 31 Fitting at the outlet of the pump head Remove the solvent line from the solvent inlet Disconnect the tubes of the piston backflushing from the flush pump and the pump head Fig 32 Tubing of the piston backflushing KNAUER P 6 1L User Manual V6890 Version 1 4 50 Maintenance and Care Using the Allen wrench unscrew the 4 screws subsequently by one turn Fixate the pump head with your hand and remove the screws Lift off the pump head Fig 33 Screws of the pump head Next steps Maintain the check valves or install a new pump head Check Valves Clogged check valves do not open and close properly They cause pressure fluctuations and irregular flow If it is impossible to clean the check valves replace the whole unit Procedure Remove the check
2. Setup Pump type Quaternary analytical HPLC pump with degasser Variants of the 5 ml min stainless steel pump head 10 ml min stainless steel 10 ml min ceramic 4 channels Teflon AF Special feature Automatic adaption of LPG cycle time Gradient formation Gradient forma Low pressure quaternary mixing tion Gradient range 0 100 1 99 recommended Minimum 0 1 increment LPG gradient 0 3 measured at 1 ml min accuracy 150 bar ethanol caffeine tracer 2 96 1 99 96 measured at 5 50 96 of the flow range water caffeine tracer LPG gradient 0 1 96 RSD measured at 1 ml min precision 0 5 96 RSD overall based on retention time at constant room temperature Mixer Mixing volume 50 100 200 ul 250 ul metal free Delay volume 210 ul dependent of mixer 410 ul metal free KNAUER P 6 1L User Manual V6890 Version 1 4 57 Technical Data Pump Heads Flow rate range 5 ml pump head 0 001 ml min 2 m min 10 ml pump head 0 001 ml min 10 ml min 0 02 10 ml min recommended 50 ml pump head 0 001 ml min 50 ml min 0 1 ml min 40 ml min recommended Maximum delivery 5 ml pump head 100 Mpa 1000 bar 14504 psi up to pressure 2 ml min 70 MPa 700 bar 10150 psi 10 ml pump head Stainless steel 70 Mpa 700 bar 10150 psi up to 5 ml min 40 MPa 400 bar 5800 psi Ceramic 40 MPa 400 bar 5800 psi 50 ml pump head 20 MPa 200 bar 2900 psi P 6 1L General Solvent conveyance Variant
3. Levels passive 5 V active 0 V Start IN TTL input Low active Secure switching threshold at least 10 mA After receiving a signal short circuit to ground from an external device the device starts If controlled with software an elec tronic trigger is send through the LAN Error OUT TTL output Levels passive 5 V active 0 V Error IN TTL input Low active Secure switching threshold at least 10 mA After receiving a signal short circuit to ground from an external device an error message appears and the device stops Error GND Reference point of the voltage at the signal inputs 24V Event controlled switching of 24 V against GND Protection 24 V 200 mA 5V Provides a voltage of 5 V with respect to GND This makes it possible to supply a consumer that is switched by an EVENT Protection 5 V 50 mA GND Reference point of the voltage at the signal inputs Events Connector For test purposes or in some other cases it can make sense to manually enter these signals Sending control signals Events to external devices Opening and closing contacts Activating 500 ms pulses KNAUER P 6 1L User Manual V6890 Version 1 4 31 Installation Legend Events Pepe none n Il Events Remote Con 0000008 O OOOOO O L nector Ll E al N QJ iO el zou Ou EE o m UH sr in O 12 345678 91011 12 Fig 27 Relation between display and Events t
4. contact the Techni cal Support Cannot start time table Check the data on the device s display or in the opened pro gram Cannot use non existing Check if the channels to be component mixed are set correctly in the Setup section of the device or in the Instrument Setup of the chro matography software Cannot write data on Restart the device If this system FRAM message occurs repeatedly con tact the Technical Support KNAUER P 6 1L User Manual V6890 Version 1 4 4 Troubleshooting Component settings not Change the Setup settings or compatible with gradient change the gradient in the pro setup gram or in Setup E Error input activated Eliminate the short circuit con nected to Error In F Flow max in the program When entering the flow note is not compatible with the the maximum flow of the pump current pump head head and only enter values inside this range GUI communication failed Restart the device If this system message occurs repeatedly con tact the Technical Support C H HPG B component not present HPG B Command time out HPG B incompatible pump head type HPG B Service active Instrument remote This entry is not executable controlled Quit software Invalid index in time Change the entry in the pro table gram line Invalid line number Invalid link Create a link or use an existing link L Leak sensor not present R
5. monitor and save all important parameters and settings RFID technology offers the following advantages Software automatically recognizes the parameters of the pump head All service relevant data of the pump head is stored Volume of the mixer is 100 ul The mixer is exchangeable and can be replaced by mixers with a volume of 50 ul or 200 ul The parameters necessary for the mixer are set via Control Unit or via chromatography software 50 ul 100 ul 200 ul 250 ul 1000 bar 400 bar The piston backflushing function automatically flushes the rear piston area of the pump head upon switch on and in continuous mode Upon switch on The rear piston area of the pump head is automatically flushed for 15 seconds n continuous mode The rear piston area of the pump head is flushed automatically every 15 minutes for 15 seconds This mode protects the column from excessive fluctuations in pressure If the maximum pressure is reached the user can decide if the pump should be switched off or continue running with constant pressure You find a detailed description on how to use this option in the respective software manual The Control Unit the Mobile Control and the different software products e g ClarityChrom or OpenLAB can be used to dis play or read out the GLP data of the pump You find a detailed description on how to display or read out GLP data in the respective user manuals The following optional accessori
6. A9846 3 P6431 P6432 P6424 P6425 AZDOO FZAO2 FPH30 FPH34 Solvent tray AZURA solvent tray E 2 1L Drainage system Corrugated hose 16 cm PE gray Corrugated hose 150 cm PE gray Funnel Exhaust Capillary guide Capillary guide top Capillary guide side Control Unit Control Unit CU 2 1 incl user manual Accessories kit AZURA accessories kit Accessories kit P 6 1L isocratic Accessories kit P 6 1L quaternary KNAUER P 6 1L User Manual V6890 Version 1 4 Product riser Tool Power cable Colored side panels User Manual Qualification documents Disposal Accessories kit P 6 1L binary AZURA tool kit Capillary cleaning kit Metal capillary cutter AZURA product riser 28 mm s Switzerland Side panel White Knight white Side panel Deep Sea dark blue Side panel Sparkling Meadow green Side panel Imperial Dignity bordeaux Side panel Naked Sword silver Side panel Melting Copper copper User manual pump P 6 1L Installation qualification DE VIQP61L Operation qualification DE VOQP61L 1 Please note that only KNAUER authorized personnel and companies may open the devices To have to side panels exchanged contact the Technical Support Disposal KNAUER Hand in old devices or disassembled old components at a certi fied waste facility where they will be disposed of properly AVV Marking in Germany According to the German Abfallverzeichnisverordnung AVV January 2001 old devices manufactured by
7. If this system message occurs repeatedly contact the Tech nical Support S SetPoint in the program is Note the maximum pressure of not compatible with the the pump head current PH PMax T This link is used in First quit or delete wakeup pro WAKEUP gram wu Wake Up then edit or delete link This program is used ina First pause or delete the link link then edit or delete data using the chromatography software This program is used in First quit or delete wakeup pro WAKEUP gram wu Wake Up then edit or delete data by means of the chromatography software Too many lines in Check the number of program program lines A maximum of 100 program lines are possible KNAUER P 6 1L User Manual V6890 Version 1 4 44 Maintenance and Care Unable to attain min Confirm pump continues run flow setpoint ning Unable to attain pressure The entered pressure cannot be setpoint achieved with the maximum flow set in the Constant Pressure mode Check for leaks Increase the upper flow level c Reduce the working pressure Unknown pump head Check the pump head type Check whether a pump head with RFID recognition has been installed If this system message occurs repeatedly contact the Tech nical Support W Wake up time already Correct the entry for date or passed otherwise time Maintenance and Care Organic solvents are toxic above a certain concentration Ensure that wo
8. KNAUER are marked as waste electrical and electronic equipment 160214 WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German Elektroaltger teregister EAR The number belongs to category 8 and 9 which among others comprise laboratory equipment P 6 1L User Manual V6890 Version 1 4 62 Legal Information All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End users can send their old devices manufactured by KNAUER back to the dis tributor the importer or the company free of charge but would be charged for the disposal Solvents and Other Operating Materials All solvents and other operating materials must be collected sep arately and disposed of properly All wetted components of a device e g flow cells of detectors or pump heads and pressure sensors for pumps have to be flushed first with isopropanol and then with water before being maintained disassembled or disposed Legal Information KNAUER Warranty Conditions The factory warranty for the device is valid for 12 months after the date of dispatch All warranty claims shall expire in the event that any unauthorized changes are made to the device During the warranty period any components with material or design related defects will be replaced or repaired by the manu facturer free of charge This warranty excludes the following accidenta
9. Languages Available by telephone in German or English 8 am to 5 pm CET Phone 49 30 809727 111 Telefax 49 30 8015010 support knauer net Maintenance Contract The following maintenance work on the device may only be per formed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract Opening the device or removing housing parts Maintenance Intervals The Control Unit the Mobile Control and the different software products e g ClarityChrom or OpenLAB enable you to dis play or read out the operating time of the pump You find a detailed description on how to display or read out GLP data in the respective user manuals 1000 Check the torque of the screw fit tings Clean the pistons of the pump Check the check valves of the pump head 5000 Replace all seals Clean the check valves of the pump head 10000 Replace pump head spare parts Replace the check valves of the pump head P 6 1L User Manual V6890 Version 1 4 46 Auxiliary material KNAUER Maintenance and Care Cleaning and Caring for the Device Device defect Intruding liquids can cause damage to the device Place solvent bottles next to the device or in a solvent tray Moisten the cleaning cloth only slightly All smooth surfaces of the device can be cleaned with a mild commercially available cleaning solution or with isopropanol Checking the Fittings Check if all fittings are tight If fitti
10. TE See 18 Connecting the Pump Head to the Solvent enan 19 bibat PHP quei Bardo deut demus tes Exc dno ate Rer A ICE 20 Connecting the Pump Head to the Solvent enan 20 Connecting the Solvent Selection Valve to the Solvent 21 Ouatermnary POMPES SENS MAR UN EE eee EEG EM 22 Connectirig the Degass sex cn as ee s qoo estaban Ae AUS Eu Vr esas 22 Connecting the Pump Head to the Solvent ena 22 Connecting the 4 Channel Degasser to the Solvent 23 Connecting the Valve BIOCK 52 ves E ER A ERR ERR tete 24 Connecting the Piston Backflushing 24 Connecting the Leak Management 25 KNAUER P 6 1L User Manual V6890 Version 1 4 4 Table of Contents COMORES tdi ot D a order s Cor Mo ii 26 Connecting the Device to a Computer 26 Configuring the LAN Settings 27 Connecting ING Cables tad 7 00 duke obe Ser dire dede desc case 27 GConliguring he ROUL oo 9 mare E E RE Pes a RS EG retient 28 Integrating the LAN into a Company Network 28 Controlling Several Systems Separately in a LAN 28 Pin Header CONN CION tain 49 4 3 08 RE d mod UE om E RU SOR wee 29 KEMOL COMMEC ON scuta win aod Cute tetra ati audacia urtica ni dod et iter auia e ee 29 Explanations for Remote Connector 29 EVENTS CONN CLON uet d Eu Ee dta uaa deas
11. be ee did 59 Accessories and Spare Parts 60 Disposal 2 045 ema mine ele nent ee eee Ge eee ua eee ene 61 Legal Information 23999 E edicere eae ered ae ede 62 Waman y Condis scd a a ala E RKR RTE I tb 62 Transport Damage 2 43 24 45444 Neve Ia D UMR NI I DM E De e Rae ad e b N 62 HPLC GIOSSdl Y vin sett tie ah ee ae D paid das ci oe one 63 Tee ree ins eae ae ee ee deen pe 65 Declaration of Conformity 68 To whom it may concern In case you prefer a French language user manual for this prod uct submit your request including the corresponding serial number via email or fax to KNAUER support knauer net 49 30 8015010 Thank you A qui que ce soit Si jamais vous pr f riez un manuel en francais pour ce poduit contacter KNAUER par email ou par fax avec le no de s rie support knauer net 49 30 8015010 Merci beaucoup KNAUER P 6 1L User Manual V6890 Version 1 4 6 Intended Use Description Scope of application Isocratic pump Legend Pressure sensor Pump head Binary pump KNAUER Intended Use Note Only use the device for applications that fall within the range of the intended use Otherwise the protective and safety equipment of the device could fail The pump P 6 1L is suitable for analytical and semi preparative applications The device can be used in the following areas Biochemistry analysis Chem
12. can only be de aerated when switched on because the syringe can only take in liquid in Flow on mode Only then are the valves of a binary pump opened For the binary pump the de aerating process may take a while during initial start up because the solvent tubes are filled with liquid for the first time The installation has been completed The capillary and tubing have been connected The pump has been switched on Syringe with Luer lock Column defect Damage to the column due to de aeration Open the venting screw or remove the column P 6 1L User Manual V6890 Version 1 4 35 Procedure Next steps Result Prerequisite Procedure Next steps KNAUER Operation Process Open the venting screw of the pressure sensor With the syringe extract fluid through the de aeration port Fig 28 Venting screw of the pressure sensor Start the pump via the Control Unit or the software option Purge with a medium flow rate 50 of the maximum flow If the extracted fluid flows continuously stop suction and close the venting screw After de areating excess air is removed from the pump head and the capillary Switch On Note Prior to switching on the pump you should de aerate it to remove air from capillary and tubing After switching on the pump the piston backflushing automati cally flushes for 15 seconds The installation has been completed Device defect Changes
13. connector is located on the side of the device Fig 25 Control Unit port Connecting the Device to a Computer This section describes how to set up an HPLC system in a local area network LAN and how a network administrator can inte grate this LAN into your company network The description applies to the operating system Windows and all conventional routers Note To set up a LAN we recommend to use a router That means the following steps are required Process 1 On the computer go to the control panel and check the LAN properties 2 Hook up the router to the devices and the computer 3 On the computer configure the router to set up the network 4 Install the chromatography software from the data storage device 5 Switch on the device and run the chromatography software P 6 1L User Manual V6890 Version 1 4 27 KNAUER Installation Configuring the LAN Settings The LAN uses only one server which is normally the router from that the devices automatically receive their IP address Prerequisite n Windows power saving hibernation standby and screen saver must be deactived n case you use an USB to COM box the option Allow the computer to turn off ths device to save power in the device manager must be deactivated for all USB hosts Only for Windows 7 For the network adapter the option Allow the computer to turn off this device to save power in the Device Manager must be deactiva
14. flow system KNAUER P 6 1L User Manual V6890 Version 1 4 22 Capillary tubing lay out Piston backflushing Flow path solvent Prerequisites Material KNAUER Installation Quaternary Pump The figure shows the installation plan for capillary and tubing on the quaternary version of the pump If you own a different ver sion of the pump see the corresponding chapter for installation Instructions The gray lines represent the connection of the eluents to the pump and the black lines represent the connection of the piston backflushing Fig 14 Installation plan for the quaternary pump The flushing solution is re used Since the flow path is circular only one bottle is used for the flushing solution Connecting the Degasser The degasser outlet is readily connected to the valve block The four solvents are connected with the inlet of the degasser The solvent mixture is conveyed from the degasser via the valve block into the pump head From the pump head it is taken to the mixer The mixer is connected to the HPLC system Connecting the Pump Head to the Solvent The device has been switched off The power plug has been pulled The front cover has been removed Flangeless fitting Device defect The pump head can get damaged when the inlet and outlet are blocked Remove the cap fittings from the inlet and outlet of the pump head prior to use P 6 1L User Manual V6890 Version 1 4 23
15. of the environmental temperature cause condensation inside the device Allow device to acclimate for 3 h before connecting to power supply and taken into operation Component defect Damage to the pump head in case it runs dry Ensure that liquid runs through pump head and piston back flushing Connect the device to the power supply Switch the power supply on Wait until the pump has completed the self test A WU N gt Start the pump at a medium flow rate Operate the pump Control There are several options for controlling the device Chromatography software P 6 1L User Manual V6890 Version 1 4 f Rere LegendLegend Left LED Center LED Right LED Power switch 1 36 Standby KNAUER Operation Control Unit App Mobile Control Note It is not possible to use two options simultaneously If the device is connected to the software it cannot be controlled via Control Unit or Mobile Control etc Control with Chromatography Software To control the device with software you have to establish a con nection between the LAN port and a computer Devices can be controlled with e g OpenLAB EZChrom edition version A 04 05 or higher or with ClarityChrom version 5 0 2 or higher You find a detailed description on chromatography soft ware in a corresponding user manual Control with Control Unit To control the device using the Control Unit connect the cable of the
16. the sections Operating Environment and Technical Data as well as the ambient conditions of other devices to be integrated into that system Device defect Damage to the pump head caused by overtightened capillary fittings Note the torque of the fittings Use 5 Nm torque for stainless steel fittings Use 0 5 Nm torque for PEEK fittings Torque wrench Stainless steel fittings are tightened with 5 Nm PEEK fittings are tightened with 0 5 Nm Isocratic Pump The figure shows the installation plan for capillary and tubing on the isocratic version of the pump If you own a different version of the pump see the corresponding chapter for installation Instructions The gray lines represent the connection of the solvents to the pump and the black lines represent the connection of the piston backflushing P 6 1L User Manual V6890 Version 1 4 19 Capillary layout Piston backflushing Solvent flow path Prerequisites Material Procedure Next steps KNAUER Installation Fig 9 Installation plan for the isocratic pump The flushing solution is re used Since the flow path is circular only one bottle is used for the flushing solution The pump head takes the liquid in from the bottle and conveys it to the pressure sensor Connecting the Pump Head to the Solvent The device has been switched off The power plug has been pulled The front cover has been removed Flangeless fitting Device de
17. valve Clean the check valve Install the check valve Removing the Check Valve The pump head is equipped with two check valves Prerequisite The pump head has been purged The capillary and tubing have been removed The pump head has been removed Tools Open end wrench size 13 KNAUER P 6 1L User Manual V6890 Version 1 4 51 Maintenance and Care Unscrew and remove the capillary connector e Loosen the outlet fit ting with the open end wrench Remove the first check valve Loosen the inlet fitting 3 with the open end wrench Remove the second check valve Fig 34 Check valve in pump head Next steps Clean the check valves Cleaning the Check Valves In order to be cleaned the check valves are not disassembled but are cleaned as a whole Prerequisite Both check valves have been removed Procedure Process Place each check valve into a beaker with solvent e g iso propanol Put the beaker with the check valve in an ultrasonic bath for at least 10 minutes Let the check valves dry afterwards Next steps Insert both cleaned check valves Installing the Check Valve Insert the check valves in the direction of the flow The notch of the check valve points downward Normal phase Insert the check valves in the direction of the flow The arrow on the check valve points upward Prerequisite The check valves have dried Component defect Damage to the thread
18. 6 Loosen the capillary below the inline filter KNAUER P 6 1L User Manual V6890 Version 1 4 53 Next steps Prerequisite Tool Procedure Next steps KNAUER Maintenance and Care 3 Loosen the fitting of the inline filter with the open end wrench size 13 and remove manually Remove clogged inline filter cartridge Fig 37 Loosen the inline filter below the pressure sen sor Insert the new inline filter cartridge Inserting the new Inline Filter Cartridge The flow direction is designated on inline filter cartridges The inline filter and fitting are inserted into the pressure sensor in such a way that the designating notch always points upwards None Torque wrench Component defect Damage to the threads of components caused by overtightened fittings Pay attentio to the torque values Use maximum 5 Nm for stainless steel fittings Use maximum 1 Nm or finger tight and an additional quarter of a turn with appropriate pliers for PEEK fittings 1 Insert the inline filter cartridge with the des ignating notch point ing upwards into the fitting Manually screw the fit ting with the inline fil ter cartridge in the pressure sensor and tighten with a torque Fig 38 Inserting the inline fil wrench using the ter cartridge into the fit appropriate torque ting Reinstall the capillary at the pressure sensor below the fitting of the inline filter P 6 1
19. 9 Q Quaternary pump 6 7 22 64 delay volume 56 mixing volume 56 technical data 56 R Repeat Orders 59 RFID 9 chip 9 P 6 1L User Manual V6890 Version 1 4 67 router LAN 28 S safety equipment 12 Safety for users 11 Salts 9 Service 45 Service Interface 7 Setup 16 Software 9 45 Solvent 9 64 change 46 inlet 49 line 49 solvent flammability 12 line 12 self ignition point 12 tray 12 Solvent bottles connect 17 Solvent conveyance 57 Solvent Selection valve 21 64 technical data 55 Spare parts 11 Stainless steel 9 Storage pump head 48 Switch on 35 System Messages 40 T Technical data binary pump 55 degasser module 58 delay volume 55 56 flow rate 57 general 57 gradient formation 55 56 isocratic pump 55 maximum pressure 57 mixer 56 mixing volume 56 quaternary pump 56 solvent conveyance 57 Solvent Selection valve 55 weight 55 Index Torque 45 46 51 53 54 transport damage 62 Troubleshooting LAN 38 U Unpacking 16 V Valve 64 Valve block 24 Venting screw 6 7 47 W warranty 62 Weight 55 Technical Support see Hotline 14 45 test Installation Qualification 37 Operation Qualification 37 KNAUER P 6 1L User Manual V6890 Version 1 4 Ih KNALIER Declaration of Conformity Producer Model Type Reference Machinery EMC Disposal Safety CE revision 03 Date KNAUER Wissenschaftliche Gerate GmbH Hegauer Weg 38 14163 Berlin Deu
20. A steel pump head Pump P 6 1L binary with 10 ml stainless APH35EA steel pump head Pump P 6 1L binary with 10 ml ceramic APH65EB pump head Pump P 6 1L binary with 10 ml stainless APH35ED steel normal phase pump head Pump P 6 1L quaternary with 5 ml stain APH34GA less steel pump head Pump P 6 1L quaternary with 10 ml APH34EA stainless steel pump head Pump P 6 1L quaternary with 10 ml APH64EB ceramic pump head KNAUER P 6 1L User Manual V6890 Version 1 4 60 Repeat Orders Accessories and Spare Parts Order number AHA60 AHB40 AHB32 AHB40BA Pump head Pump head 5 ml stainless steel Pump head 10 ml stainless steel Pump head 10 ml ceramic Pump head 10 ml for normal phase Stainless steel AHC20 AHC22 G0924B Pump head 50 ml stainless steel Pump head 50 ml ceramic Check valves Check valve unit for AZURA 5 10 ml pump head ruby sapphire Check valve unit for AZURA 5 10 ml pump head normal phase spring sup G0563 5 ported ruby sapphire Check valve unit for AZURA 50 ml pump G0924C head ruby sapphire G1451 G1451A G1452 Inline filter Inline filter cartridge 10 50 ml vol 60 ul Inline filter cartridge 10 50 ml vol 20 ul Inline filter cartridge 10 ml vol 60 ul bio Inline filter cartridge 10 50 ml vol 20 ul G1452A bio Mixer AZURA mixer 50 ul AZURA mixer 100 ul AZURA mixer 200 ul AZURA mixer 250 ul bio AZZOOMB AZZOOMC AZZOOMD AZZ10ME AZCOO A9846 1
21. Control Unit to the device The Control Unit is an optional accessory You find a detailed description on the Control Unit in its accompanying user manual Control with Mobile Control The Mobile Control is an app which can be installed on your computer or tablet computer To control the device using the Mobile Control connect the computer or tablet computer to a wireless LAN router You find a detailed description on the Mobile Control in its accompanying user manual Meaning of the LEDs There are three LEDs and a switch on the front of the device Fig 29 LEDs and switch on the front of the device The LEDs can have different colors depending on the operating conditions To start the standby keep the switch pressed for 5 seconds Note Malfunctioning system after repeated standby possible After repeatedly using the standby switch off the power switch and back on again to reset the data storage P 6 1L User Manual V6890 Version 1 4 37 Functionality Tests IQ and OQ tion Left LED red Error Message Check the sys tem Shortly press the switch to deactivate the error message Center LED does not light Device has been Switch on the switched off device flashes green Device not ready Wait until the for operation device is ready green Device is switched on Right LED green Device active or ready for measur ing blue Device in standby Press the switch to end the standby Function
22. EFIOS sia renee er bee ee ERU EX Past E AN EN X E 46 Fus siz h sa PUMP de ducis Den de aetas eder aep dece de Den ec dn beet aet ns 46 Putting the Pump Out of Operation 47 bor MC rr 47 Disconnecting from Power Supply 48 Preparing the Pump Head for Storage 48 Dismounting the Pump Head 48 KNAUER P 6 1L User Manual V6890 Version 1 4 5 Table of Contents CHICGK VAIN GS Gnas nie bugs oe Go ro ee eee Loin 50 Removing the Check Valve i eR ew Gia ewes code ed 50 Cleaning the Check Valves 4 esaeim ds Seam iet e dos SE e pou 51 Installing the Check Valve iud auto bo dw ed bu dc Io ras qoe do he 51 Replacing the INline BILE 5 5 5 5 3492 3 cage S MES oi R ce HE oS Sar 52 Removing the Inline FILE 23h EP VeEPES Roca oe TE E E x 52 Inserting the new Inline Filter Cartridge 53 Replacing trie MIXED e esc ed E wade alos e ORE E SCA eee eee ee HE QS 54 REMOVING TEE HM Tm 54 Technical Data 2e rer decern ra do SES Up ad esa d a dean 55 XEM eren 55 dcr PC ECCE 55 POS TU COULePDIdV bcn aE coeant a ed Ogee taf Bee E s ca an 56 PUD ele 4 4 a8 dose RS Salen tod eee eee eee QUU b Gio a ss sedent 57 Po ME Gellerals iesus m Bark dot antes ws FOE BA ae Ow BES VON ot 57 Repeat OFdeFS ust bo ananas e do EU df ao OD A fonts male 59 MOGUICS esseer oe dedit ba CE Ped ut anite dun no ofer ee detur
23. Il KNALIER feura Pump P 6 1L User Manual V6890 3 Table of Contents Table of Contents Note For your own safety read the manual and always observe the warnings and safety information on the device and in the manual Intended H Use beau whee ee ee ECKE BIS VEO Abe eap Pob oo 6 DEVICE Vaani ER Ne ps Jo stat ans ee enc ay fae do tev aon E ee Bae 6 FIDPIE VOW te Red dg tuo la hace A races ub ob nod Bete Gig ed Mae pa Sede 6 Ront VEW c xd uxo eub og re vd NOR ee n ROO Oe ee ae Oe ee 7 SIGS VICW a noeud ES ae ee wee Sco tae oret tenda 8 EI cats p oe ACE aede TE ee oN se er ree oe 8 SONEM Libraries mamie Da eee Te Pro ANM OSE dog dd 9 Scope Ol DEVE uide oe zoe aie ere io us Be eee ee 11 Safety TOF Users co repa we P eC REV EE Oe EE S ades 11 SIGMA VY OO Me TP rper TIPP 13 Decontamibiallol Su 2a v d nia yond Aah eee SU Pasta god d utro eode dd 13 Symbols and SIGNS s ese 5 ase carere ud aes SO AER MORE eee eee ee 14 ipe AUION P cr 14 cuc Acer 14 Operating ENVIOnNMENT ae d ko e oe em 989 994 Een A eae QR d 15 SOIUIDL sachs D pte dn to on 16 Bi ere 2 SR hte Shana ee aes Coe TTE 16 Capillary ang FINOS eierne S ar eoe ESCAS qe Aristide reine 17 Connecting the Solvent Bottles 17 Pre Iristall d Capillary woe acide dun ae OE Roe har SU HEAT ses 17 Integrating the Pump into an HPLC Flow System 18 Sa BERI a E 9 R NOR tet Solos R te paa Oca acu bata doi Spe Se ea
24. L User Manual V6890 Version 1 4 54 Procedure Tools Procedure Next steps Prerequisite Auxiliary material KNAUER Maintenance and Care Replacing the Mixer A clogged mixer can cause pressure fluctuations and irregular flow The mixer is replaced completely as an assembly 1 Remove capillary from the inlet and outlet of the mixer 2 Remove the screws from the front of the mixer 3 Remove the mixer 4 Install new mixer Open end wrench size 1 4 Allen wrench 2 mm and 2 5 mm Torque wrench Component defect Damage to the threads of components caused by overtightened fittings Pay attentio to the torque values Use maximum 5 Nm for stainless steel fittings Use maximum 1 Nm or finger tight and an additional quarter of a turn with appropriate pliers for PEEK fittings 5 F Remove capillary at the inlet and outlet of the mixer laa Remove the screws reine from the front of the mixer and remove the mixer Install new mixer 4 Manually screw the fit tings of the capillary in the mixer T ighten with a torque wrench using the appropriate torque Fig 40 Mixer bio Bring the device into operation again Removing a Leak If liquid enters the inside of the device switch off the device If this is not the case it is not necessary to switch off the device Cloth for drying the leak sensor P 6 1L User Manual V6890 Version 1 4 55 Technical Data
25. Manual V6890 Version 1 4 65 Index Numerics 2 channel degasser 6 4 channel degasser 7 23 A Accessories 11 Acetonitrile PEEK 18 Additives 9 Ambient conditions 15 48 Analog port 33 AVV marking 61 B Binary pump 6 18 20 63 delay volume 56 mixing volume 56 Bio inert 6 8 9 Biopurification 9 C Cap fitting 19 20 21 22 Capillary 17 63 pre installed 17 Care 46 Ceramic 6 Check valve 50 63 accessories and spare parts 60 clean 46 51 install 51 remove 50 Checking the fittings 46 Chromatogram 63 Chromatography software 9 ClarityChrom 9 36 45 Cleaning 46 Column 63 Contact data 45 Control 26 35 chromatography software 36 Control Unit 26 36 Mobile Control 36 Control Unit 8 9 26 36 45 63 D Dead volume 63 declaration of conformity 68 decontamination 13 Degasser 11 22 63 KNAUER Index Degasser module technical data 58 Degassing chamber 23 Degree of protection 58 Delay volume binary 56 isocratic 55 quaternary 56 Detector 63 Device care 46 connect to computer 26 Dimensions 58 E Electrical connections Events terminal strip 30 remote terminal strip 29 Eluent 63 F Fitting flangeless 17 Fittings 17 Flangeless fitting 17 63 Flow rate 57 Flushing solution 11 FPLC 9 Functionality tests 37 G GLP 63 data 9 Gradient 63 Gradient formation binary 55 quaternary 56 Ground 63 H High pressure side 64 Hotline 14 HPG pump 6 63 HPLC 9 64 Initial
26. Procedure Next steps Prerequisite Material Procedure Next steps KNAUER Installation 1 Slide the flangeless fit ting onto the tubing 2 Insert the tubing into the free inlet on the bottom of the pump head 3 Tighten the fitting by hand Fig 15 Solvent line on the pump head Integrate the pump into the HPLC flow system Connecting the 4 Channel Degasser to the Sol vent The 4 channel degasser contains four degassing chambers Each degassing chamber has an inlet and an outlet on the front of the pump The device has been switched off The power plug has been pulled The front cover has been removed Flangeless fitting NOTICE Device defect Very high pressures can damage the degasser membrane The membrane can withstand a maximum pressure of 7 bar Never connect the degasser to the pump outlet 1 Connect the tubing from the four solvent bot tles to the inlets A B C and D of the degasser see Seal inlets not in use with cap fit tings Fig 16 4 channel degasser with valve block Integrate the pump into the HPLC flow system P 6 1L User Manual V6890 Version 1 4 24 Installation Connecting the Valve Block Note Note the instructions on how to install the flangeless fit ting in chapter see Capillary and Fittings on page 17 The valve block inlets are pre installed If the central outlet connection has to be changed e g you wa
27. Procedure Process 1 Remove the leak 2 Dry the leak tray 3 Acknowledge the system error via Control Unit Mobile Control or the standby button only in standalone mode Next steps Bring the device into operation again Technical Data P 6 1L Isocratic Setup Pump type Isocratic analytical HPLC pump Variants of the 5 ml min stainless steel pump head 10 ml min stainless steel 50 ml min stainless steel 10 ml min ceramic 50 ml min ceramic Delay volume 60 ul for 10 ml min P 6 1L Binary Setup Pump type Binary analytical HPLC pump with degas ser Variants of the 5 ml min stainless steel pump head 10 ml min stainless steel 10 ml min ceramic 10 ml min stainless steel for normal phase applications Degasser 2 channels Solvent Selection 2 x 2 channels valve Gradient formation Gradient forma High pressure binary mixing tion Gradient range 0 100 96 5 95 96 recommended Minimum incre 0 1 96 ment KNAUER P 6 1L User Manual V6890 Version 1 4 56 Technical Data HPG gradient 0 3 measured at 1 ml min 150 bar accuracy ethanol caffeine tracer 1 96 5 95 96 measured at 0 1 10 ml min water caffeine tracer HPG gradient 0 1 96 RSD measured at 1 ml min precision 0 3 96 RSD overall based on retention time at constant room temperature Mixer Mixing volume 50 100 200 ul 250 ul metal free Delay volume 110 ul dependent of mixer 410 ul metall free P 6 1L Quaternary
28. ality Tests IQ and OQ Installation Qualification IQ The customer may request the Installation Qualification which is free of charge In case of a request the Technical Support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation The Installation Qualification is a standardized document that comes as part of the delivery and includes the following confirmation of flawless condition at delivery check if the delivery is complete certification on the functionality of the device Operation Qualification OQ The Operation Qualification includes an extensive functionality test and must be purchased from the manufacturer Contact the KNAUER Sales Department to request an offer The Operation Qualification is a standardized KNAUER document and includes the following definition of customer requirements and acceptance terms documentation on device specifications device functionality check at installation site Test Intervals To make sure that the device operates within the specified range you should test the device using the Operation Qualifica tion at following intervals KNAUER P 6 1L User Manual V6890 Version 1 4 38 Troubleshooting Every 3 months average useful life of more than 5 days week or 24 hours day when operating with buffer solutions or other salt solutions Every 6 months average useful life of 1 to 5 days week Execution The test c
29. an be carried out either by the Technical Support of KNAUER or from a provider authorized by KNAUER Troubleshooting First measures KNAUER Check all cables Check all screw fittings 1 2 3 Check whether air has gotten into the supply lines 4 Check device for leaks 5 Pay attention to system messages LAN Go through the following steps in case no connection between the computer and the devices can be established Check after each step if the problem is solved If the problem cannot be located call the Technical Support 1 Check the status of the LAN connection in the Windows task bar fal Connected El Connection not established If no connection was established test the following Is the router switched on Is the patch cable connected correctly to the router and the computer 2 Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices 3 Check all connections Are the patch cable connected to the LAN ports and not the WAN port Are all cable connections between devices and router cor rect Are the cables plugged in tightly 4 If the router is integrated into a company network pull out the patch cable from the WAN port Can the devices communicate with the computer even though the router is disconnected from the company net work 5 In case you own a Control Unit check the settings in the menu Setup Ne
30. anual V6890 Version 1 4 11 Scope of Delivery Solvents not suitable for degassers Azides Benzene Carbon dioxide liquid 99 999 CO Concentrated mineral and organic acids Concentrated bases Dilute sodium hydroxide 1 M Halogenated hydrocarbons e g Freon Hexafluoroisopropanol Hexanes 60 n Hexane Hydro fluoro solvents Perfluorinated solvents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin Solvents containing particles Flushing solution These are the recommended flushing solutions e g for the pis ton backflushing Water Mixture of 80 96 water and 20 96 ethanol sopropanol Scope of Delivery Note Only use spare parts and accessories made by KNAUER or a company authorized by KNAUER Delivery Pump P 6 1L AZURA accessories kit P 6 1L accessories kit User manual English German Installation Qualification Safety for Users Professional Group The user manual addresses persons who are qualified as chemi cal laboratory technicians or have completed comparable voca tional training The following knowledge is required Fundamental knowledge of liquid chromatography Knowledge regarding substances that are suitable only to a limited extent for use in liquid chromatography Knowledge regarding the health risks of chemicals KNAUER P 6 1L User Manual V6890 Version 1 4 12 KNAUER Safety for Users Participation during an installation of a devic
31. ber Polypropylene and stainless steel Vacuum pump Low hysteresis behavior LAN Analog and event control Communication Control Control Unit Mobile Control Analog input 0 10 V Analog control Flow rate input Level event 8 event outputs TTL OC Relais and outputs 24 V Programing 19 programs 9 links 1 WAKE UP program Technical parameters GLP RFID pump head recognition detailed report Display 3 LEDs Degree of protec IP 20 tion Dimensions 361 mm x 208 2 mm x 523 mm width x height x depth Power supply Input 100 240 V Output 50 60 Hz P 6 1L User Manual V6890 Version 1 4 KNAUER 59 Repeat Orders Maximum power 100 Watt consumption Operating Environ Temperature 4 40 C 39 2 104 F ment range Humidity below 90 non condensing Height above sea maximum 2000 m level Repeat Orders Note For repeat orders of spare parts use the enclosed packing list Contact the Technical Support in case there are any ques tions on spare parts or accessories Further information Find further information regarding spare parts and accessories at www knauer net Modules Pump P 6 1L isocratic with 10 ml stain APH30EA less steel pump head Pump P 6 1L isocratic with 10 ml ceramic APH60EB pump head Pump P 6 1L isocratic with 50 ml stain APH30FA less steel pump head Pump P 6 1L isocratic with 50 ml ceramic APH60FB pump head Pump P 6 1L binary with 5 ml stainless APH35G
32. ck the number of pro gram lines a maximum of 100 program lines are possi ble Not enough space to Check the pump store program Check the number of pro gram lines a maximum of 100 program lines are possi ble Not in HPG mode Select HPG mode P Pressure max in the pro Note the maximum pressure of gram is not compatible the pump head with the current pump head Program does not exist Create and edit a program please edit the program number Program is running Quit program or wait until pro gram has been completed KNAUER P 6 1L User Manual V6890 Version 1 4 43 Troubleshooting Pump head type head Restart the device data uninitialized Check whether a pump head with RFID recognition has been installed Repeat the automatic config uration step in the chroma tography software Remove pump head clean it and re install it Pump head type Restart the device read failed Repeat the automatic config uration step in the chroma tography software Remove pump head clean it and re install it If this system message occurs repeatedly contact the Tech nical Support Pump head type RFID Pump head without RFID detec hardware not present or tion If necessary replace pump failed head Pump head type write Restart the device failed Repeat the automatic config uration step in the chroma tography software Remove pump head clean it and re install it
33. e or a training by the company KNAUER or an authorized company If you do not belong to this or a comparable professional group you may not perform the work described in this user manual under any circumstances In this case please contact your supe rior Safety Equipment When working with the device take measures according to lab regulations and wear protective clothing Safety glasses with side protection Protective gloves Lab coat What must be taken into account All safety instructions in the user manual he environmental installation and connection specifica tions in the user manual National and international regulations pertaining to labora tory work Original spare parts tools and solvents made or recom mended by KNAUER Good Laboratory Practice GLP Accident prevention regulations published by the accident insurance companies for laboratory work Filtration of substances under analysis Use of inline filters Once they have been used never re use capillaries in other areas of the HPLC system Only use a given PEEK fitting for one specific port and never re use it for other ports Always install new PEEK fittings on each separate port Follow KNAUER or manufacturer s instructions on caring for the columns More safety relevant information is listed below flammability Organic solvents are highly flammable Since capillaries can detach from their screw fittings and allow
34. echnical Support Pump head leaks Inspect the inlet and outlet screw fit tings of the pump head If the pump head seals are defective solvent enters the piston backflush ing inform the Technical Support Exchange the pump head P 6 1L User Manual V6890 Version 1 4 40 Troubleshooting Flow rate is not Check the following options correct Check the data for the solvent com pressibility Clean the check valves Exchange the check valves System error Switch off the device to reset the device s data storage and restart it after wards System Messages If other system messages are displayed besides those listed below please turn the device off and then on If this system message occurs repeatedly contact the Technical Support The system messages are in alphabetical order A A line with this time Correct the time entry already exists edit the time please C Cannot delete active pro Pause the program link Only gram link then can the link and subse quently the program used in the link be deleted Cannot edit program Pause the link Afterwards the from the running link program can be changed Cannot initialize LAN Check cables and connections in local area network Cannot operate with an Create a link empty link Cannot purge during the End method and start purging run Cannot read data from Switch the device off and on If FRAM this system message occurs repeatedly
35. ents and a description can be found in the following section Unpacking Check packaging for damage caused during transportation Utility knife Bruises Damage to the device by carrying or lifting it on protruding housing parts The device may fall and thus cause injuries Lift the device on the side of the housing only Process Set up the package in such a way that you can read the label Using the utility knife cut the adhesive tape and open the packaging Remove the foam insert Take out the accessories kit and the manual Open the accessories kit and check the scope of delivery In case any parts are missing contact the Technical Support Clasp the device from below lift it out of the packaging and place it on its feet Do not hold onto the front cover Check the device for signs of damage that occurred during transport In case you notice any damage contact the Technical Support Place the device in its site of operation and remove protec tive foil P 6 1L User Manual V6890 Version 1 4 17 Installation Next steps Store packaging and keep the included packing list for repeat orders Capillary and Fittings All tubing and capillary which connect the components of the pump are pre installed Only the solvent bottles have to be con nected and the pump has to be integrated into the flow of the HPLC system Connecting the Solvent Bottles To connect the solvent bottles tubing with pre
36. erminal strip Q for Events Connector TTTL TTL compatible output Levels passiveO V active 5 V Pulse 5V for at least 1000 ms 2TTL TTL compatible output Levels passive 0 V active 5 V Pulse 5V for at least 1000 ms 30C TTL output Levels passive 0 V active 5 V Pulse 5V for at least 1000 ms GND Reference point of the voltage at the signal inputs ATTL TTL compatible output Levels passive 0 V o active 5 V e Pulse 5V for at least 1000 ms Ll KNAUER P 6 1L User Manual V6890 Version 1 4 32 Installation STTL TTL compatible output Levels passive 0 V active 5 V Pulse 5V for at least 1000 ms 60C TTL output Levels passive 0 V active 5 V Pulse 5V for at least 1000 ms GND Reference point of the voltage at the signal inputs Relay contact The contact Is on a floating basis Its setting depends on the settings in the Control Unit or software Steady rate signal passive open relay contact o active closed relay contact e Pulse Closed relay contact for at least 1000 ms Permissible load of the relay contact 1 A 24 V DC 1 Relay contact The contact is on a floating basis Its setting depends on the settings in the Control Unit or software Steady rate signal passive openrelay contact o active closedrelay contact e Pulse Closed relay con
37. es are available Pump head cooler for the pump head Control Unit Mobile Control Solvents Even small quantities of other substances such as additives modifiers or salts can influence the durability of the materials If there is any doubt contact the Technical Support of the manu facturer P 6 1L User Manual V6890 Version 1 4 10 KNAUER Intended Use Suitable solvents Acetate buffer solutions Acetone at 4 C 25 C 39 2 F 77 0 F Acetonitrile Benzene Carbon dioxide liquid 99 999 96 CO Chloroform Dilute acetic acid e g 0 1 1 96 at 25 C 77 0 F Dilute ammonia solution Dilute sodium hydroxide 1 M Ethyl acetate Ethanol Formiate buffer solution Isopropanol Methanol Phosphate buffer solutions 0 5 M Toluol Water 1 valid for the specified temperature range 2 notrecommended in combination with PEEK small parts and PEEK capillary Diethylamine 0 1 96 DEA Dilute phosphoric acid Dimethyl sulfoxide DMSO Methylene chloride Slightly volatile solvents Tetrahydrofuran THF Triethylamine 0 1 TEA Trifluoroacetic acid 0 1 TFA 1 not recommended in combination with PEEK small parts and PEEK capillary Concentrated mineral and organic acids Concentrated bases Halogenated hydrocarbons e g Freon Perfluorinated solvents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin Solvents containing particles P 6 1L User M
38. es the single components of a substance spatially by interacting with an analyte trans ported with the solvent to different degrees Control Hand held device with a touchscreen to drive Unit single AZURA devices locally Dead vol Volume of capillary and system components ume between mixer injector and column as well as between column and detector Degasser Degasser module for fluids Detector Device measuring the composition or the quantity of a substance Eluent Flowing agent that transports the substances to be separated or isolated through the column solvent mobile phase Flangeless Fitting without a protruding edge which can fitting be fastened manually GLP Quality assurance system for laboratories Good Laboratory Practice Gradient Time dependent composition of solvent mobile phase on low pressure or high pres sure side of system Ground Protective measure for electro conductive housing parts HPG pump Pump configured to generate a gradient on the high pressure side Usually a binary pump with two separate pump units which each convey one solvent The gradient is generated behind the pump KNAUER P 6 1L User Manual V6890 Version 1 4 64 HPLC Glossary High pres Devices and capillary which are under high sure side pressure in an HPLC system HPLC High pressure liquid chromatography HPLC High pressure liquid chromatography Isocratic Mode of sample separation where the compo si
39. estart the device If the leak sensor cannot be found contact the Technical Support Leak was detected Switch off the device Remove the leak and start the device afterwards Line in time table is Edit the program line empty Link is running Wait until the link has been completed then change the link or delete it M Maximum pressure Check whether the con System stopped nected capillary and connec tors are clogged Adjust the maximum pres sure limit Restart the system KNAUER P 6 1L User Manual V6890 Version 1 4 42 Troubleshooting Minimum pressure Sys Increase the pressure or tem stopped adjust the lower pressure limit Restart the system Motor failure Restart the device If this system max current message occurs repeatedly con tact the Technical Support Motor failure position error Motor failure N No link available Pls edit Create a link and edit it link first No link available No valid pump head type Restart the device detected 2 uni pump Check whether a pump head head is set with RFID recognition has been installed Repeat the automatic config uration step in the chroma tography software Remove pump head clean it and re install it Non existing component Switch on the channel or edit is set to non 0 value the data using the chromatogra phy software Not enough space to Check the pump store link Che
40. fect The pump head can get damaged when the inlet and outlet are blocked Remove the cap fittings from the inlet and outlet of the pump head prior to use 1 Slide the flangeless fit ting onto the tubing 2 Insert the tubing into the free inlet on the bottom of the pump head Tighten the fitting by hand head Integrate the pump into the HPLC flow system P 6 1L User Manual V6890 Version 1 4 20 Capillary layout Piston backflushing Solvent flow path Prerequisites Material KNAUER Installation Binary Pump The figure shows the installation plan for capillary and tubing on the binary version of the pump If you own a different version of the pump see the corresponding chapter for installation instruc tions The gray lines represent the connection of the solvents to the pump and the black lines represent the connection of the piston backflushing Fig 11 Installation plan for the isocratic pump The flushing solution is re used Since the flow path is circular only one bottle is used for the flushing solution Every pump head can operate with two different solvents Both solvents are connected with the Solvent Selection valve The sol vents are conveyed into one pump head each and combined in the pressure sensor The pressure sensor is connected to the mixer The mixer is connected to the HPLC system Connecting the Pump Head to the Solvent The device has been switched
41. ical analysis Food analysis Pharmaceutical analysis Environmental analysis Device Variants The P 6 1L is available in three different options Isocratic pump Binary pump HPG pump Quaternary pump LPG pump All variants are available as a standard version made of stainless steel or as a bio inert version made of ceramics with PEEK capil lary PEEK connectors and PEEK venting screw Front View The isocratic pump is equipped with a pressure sensor with an integrated inline filter and a venting screw Fig 1 Isocratic pump front view The binary pump consists of two pump drives and a 2 channel degasser with a Solvent Selection valve A pressure sensor with an integrated inline filter and a venting screw are also part of the pump P 6 1L User Manual V6890 Version 1 4 7 Intended Use Legend Pressure sensor Mixer Pump head Degasser Solvent Selection valve Fig 2 Binary pump front view Quaternary pump The quaternary pump consists of a pump a valve block and a 4 channel degasser A pressure sensor with an integrated inline fil ter a venting screw and a mixer are also part of the pump Legend Pressure sensor Mixer Pump head Valve block Degasser Fig 3 Quaternary pump front view Rear View On the back of pump the connectors for LAN and power chord are located as well as the serial number of the device an the power switch on off Serv
42. ice interface Please note that the service interface is solely used for repair and maintenance tasks performed by service technicians KNAUER P 6 1L User Manual V6890 Version 1 4 Legend Serial number Interface for the Technical Support Pin header LAN port Power connection and power switch Legend Control Unit port Pump Heads Size Material KNAUER Intended Use a W K v I TEE IM UR TP WARN t bao RI 4 Fig 4 Rear view example Side View On the side of the pump the Control Unit see Control with Control Unit on page 36 connector is located Fig 5 Side View Features The pump is used to convey liquids either in an HPLC system or as a stand alone device Liquids are conveyed either with con stant flow or with constant pressure The flow and pressure which liquids can be conveyed with depend on the pump head Pump heads for several areas of application are available for the pump P 6 1L 5 ml 10 ml and 50 ml 10 ml and 50 ml Pump head with stainless Pump head with ceramic steel inlays inlays P 6 1L User Manual V6890 Version 1 4 9 Scope of Application Mixer Size Pressure Piston backflushing PMax Mode GLP data Optional accessories KNAUER Intended Use HPLC Purification FPLC Dosing applications with lon chromatography non aggressive media Every pump head is equipped with an RFID chip It is used to
43. in Putting the Pump Out of Operation The pump is designed for use with different solvents If the pump is out of operation for several weeks residual solvent can cause damage It is therefore recommended that all compo nents of the pump be flushed the solvent used be removed completely and that all components and tubes of the pump be filled with isopropanol All open connectors should be sealed Capillary and tubing which connects single components of the pump should not be removed The pump has been rinsed The pump has been switched off Open end wrench size 10 Process 1 Remove the solvent tubing and seal all open connectors with cap fittings 2 Remove the pump from the HPLC flow system and seal the open connector of the pressure sensor isocratic version or the mixer binary or quaternary version with cap fittings Choose a storage location according to the ambient conditions listed in the respective chapter of this user manual Storage Note that prior to storing all tubes and capillary should be emp tied or filled with an appropriate flushing solution e g isopro panol To prevent algae formation do not use pure water Seal all inlets and outlets with cap fittings P 6 1L User Manual V6890 Version 1 4 48 Maintenance and Care The device can be stored under the following ambient condi tions Temperature range 4 40 C 39 2 104 F Humidity below 90 non condensing Disconnecting from P
44. installed solvent filters is used The tubing is connected to the device with flange less fittings Note Do not use tools so as to not damage the fittings 1 Slide the flangeless fit ting over the tub ing Slide the lock ring over the tubing cap with the sealing ring Note the direction D of the lock ring other wise the sealing ring Fig 7 Set up of a flangeless fit could be damaged ting The thicker end of the lock ring must point into the direction of the fitting Fasten the polymer seal Manually fasten the flangeless fitting to the device Fig 8 Fully assembled flange less fitting Pre Installed Capillary Pre installed stainless steel and PEEK capillary is color coded according to its inner diameter Color Material Inner diameter red marker Stainless steel blue marker Stainless steel KNAUER P 6 1L User Manual V6890 Version 1 4 18 Tool Procedure KNAUER Installation black marker Stainless steel orange stripes PEEK Note PEEK capillary is not suitable for use with Acetonitrile Ace tonitrile can cause capillary to crack or rupture Integrating the Pump into an HPLC Flow Sys tem The pump can be integrated into an HPLC flow system by con necting the pressure sensor isocratic version or the mixer binary or quaternary version and the HPLC system with capil lary Note To integrate the pump into a system note the ambient conditions found in
45. ither switching off the power switch or by pulling the power plug Opening the Device The device may be opened by the KNAUER Technical Support or any company authorized by KNAUER only Signal Words Possible dangers related to the device are divided into personal and material damage in this user manual DANGER Lethal injuries will occur A WARNING Serious or moderate injuries can occur Minor injuries can occur NOTICE Device defects can occur Decontamination Contamination of devices with toxic infectious or radioactive substances poses a hazard for all persons during operation repair sale and disposal of a device Life threatening injuries Health danger if getting in contact with toxic infectious or radio active substances Before disposing of the device or sending it away for repair you are required to decontaminate the device in a technically correct manner KNAUER P 6 1L User Manual V6890 Version 1 4 14 Symbols and Signs All contaminated devices must be properly decontaminated by a specialist Company or the operating company before they can be recommissioned repaired sold or disposed of All materials or fluids used for decontamination must be collected separately and disposed of properly Symbols and Signs Installation Contact data Device data KNAUER The following symbols and signs can be found on the device in the chromatography software or in the user manual Electric sh
46. l or willful damage damage or errors caused by third parties that are not contrac tually related to the manufacturer at the time the damage occurs wear parts fuses glass parts columns light sources cuvettes and other optical components damage caused by negligence or improper operation of the device and damage caused by clogged capillary packaging and transport damage In the event of device malfunctions directly contact the manu facturer KNAUER Wissenschaftliche Gerate GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 111 Telefax 49 30 8015010 e mail info knauer net Internet www knauer net Transport Damage The packaging of our devices provides the best possible protec tion against transport damage Check the devices for signs of transport damage In case you notice any damage contact the Technical Support and the forwarder company within three workdays P 6 1L User Manual V6890 Version 1 4 63 HPLC Glossary HPLC Glossary Binary Pump configured to generate a gradient from pump two solvents Capillary Thin metal or PEEK pipe that connects the components and devices inside of an HPLC sys tem Check valve Valve unit built into the pump head which guides the flow of liquid from the inlet to the outlet Chromato The record of a detector signal depending on gram the flow volume of the mobile phase or time Column The column is filled with a matrix which sepa rat
47. liquid the red LED starts flashing Both the device and the data acquisition via chro matography software are stopped Prerequisite The front cover has been removed Carefully push the fun nel into the center opening of the capillary guide Abb 20 Funnel and capillary guide Push the long ending of the first nozzle into the hose Connect the nozzle and the funnel meme Push the other end of v the hose onto the noz SPA zle of the leak tray L N iN N N 5 L e ka LS Abb 22 Hose connected to device 5 For the bottom device push the short end of the nozzle into the opening in the collec tion point of the leak tray Connect the hose to Abb 23 Leak tray with nozzle the nozzle and lead the second ending to the waste bottle Place the waste bottle below the bottom device KNAUER P 6 1L User Manual V6890 Version 1 4 26 Next steps Legend Service interface Events connector LAN connector Remote connector Control Unit con nector located on the side of the device KNAUER Installation Attach the front cover Control The pump can be controlled externally in two ways Via the pin head As part of a LAN via the LAN connector of the router All connectors for external control are located on the rear side of the pump The pump can also be controlled via the Control Unit The Con trol Unit
48. n O one dud oe teda 30 Explanations for Events Connector 31 Wiring the Terminal Strip 32 Analog GOO lcs 9s iic Eres Tae ae v EDU oe eee oe grs dures Paare d 33 GA NC AS GO gee ae a e a a AA E E A E A AE E A 33 COTTON E Ge E Ta R a Gee ee 33 MAS inso SSD de Gta ae as hg Sy ae 33 AUTRE CA gl Ce ARR 34 De Aeraurid the PU e 5 R dE itr qom Scr aUe e aureo ii dis 34 SWIC ON se rsh gr 35 COMMON tr einen ha ie GS ode oe OG ae och Sacra eno 35 Control with Chromatography Software 36 Control with Control Unit 0 eee ee eee 36 Control with Mobile Control 36 Meaning or tie BEDS sects E ants Ta cs oth aaa diets aap hie lena Nr 36 Functionality Tests IQ and OQ 37 TLOUDICSHOOUING ciai reader be Mondeo dite eme eee ates 38 PIN esr cea ts A Bony Oo DO H L d 38 Possible Problems and Troubleshooting 39 SYS EMESS AGES aca ao tus e rio ci taie Door Davee eb eee A0 Maintenance and Care 44 Contact with the Technical Support 45 Maintenance Contract ss qe enous bai etras Cre dedi E reatus 45 Maintenance Intervals sr dan NR Rara Haider ler iii ERES 45 Cleaning and Caring for the Device 46 Checking sie PIEH
49. ngs are not tight re tighten fittings Not the torque applicable for each fitting in order to not damage any components Pump head inlet and outlet Stainless steel fittings PEEK fittings inline filter fittings Stainless steel fittings Stainless steel fittings Flushing the Pump Generally the pump and all its components valves degasser should be flushed after each operation Also flush the pump to clean the tubing before changing the solvent and to remove air bubbles in the capillary and tubing If buffer solution has been employed note that the buffer solution and the flushing solu tion are compatible Device defect Residuals chemicals can damage the pump head if they are not being removed before storage For reuse flush the pump head and fill it with isopropanol Before storage seal the inlets and outlets with cap fittings When buffers have been used flush with water When aggressive solvents have been used flush with isopro panol Flushing solution silicone tube P 6 1L User Manual V6890 Version 1 4 47 Procedure Next steps Prerequisite Tool Procedure Next steps KNAUER Maintenance and Care 1 Immerse the solvent tubing into the flushing solution 2 Plug a silicone tube onto the venting nozzle of the pressure sen sor 3 Open the venting screw 4 Start the pump at a Fig 30 Pressure sensor medium flow rate Bring the pump into operation aga
50. nt to install different capillary note that at least two of the outer flangeless fittings on the valve block are loosened in order to be able to install con nection in the center by hand Connecting the Piston Backflushing The piston backflushing removes salts and other substances from the area behind the pump head seals To do this connect a bottle with flushing solution to the flush pump and the piston backflushing The silicone tubing between the piston backflushing and the flush pump are pre installed Designation Inlet and outlet of the flush pump are located on the front of the device The flush pump is inside of the device and not visible from the outside A Y Fig 17 Inlet symbol Fig 18 Outlet symbol Prerequisite The pump has been set up at the site of operation The pump has been switched off Material Silicone tube 1 Plug one silicone tube onto the inlet of the flush pump and con nect to the flushing solution bottle Plug a second silicone tube onto a vacant cap illary connector of the flush pump and i connect to the flushing ses gii solution bottle Fig 19 Silicone tube between piston backflushing and flush pump KNAUER P 6 1L User Manual V6890 Version 1 4 25 Installation Connecting the Leak Management The leak management consists of the leak sensor and the drain age system The drainage system ensures that escaping liquids flow into a waste bottle If there is too much
51. ock hazard Note maximum weight loading of the leak tray during transport installation and operation A device or system marked with CE fulfills the product specific requirements of European directives This is confirmed in a Declaration of Conformity Testing seals in Canada and the USA at nation ally recognized testing centers NRTL The cer tified device or system has successfully passed the quality and security tests The chapter Installation describes all preparatory steps prior to the start up If you encounter difficulties during installation con tact the Technical Support Transport Carefully prepare the device for transport or storage If you want to return your device to KNAUER for repairs enclose the Service Request Form which can be downloaded from our website For a secure transport note the weight and dimensions of the pump see Technical Data P 6 1L User Manual V6890 Version 1 4 15 Lifiting Ambient conditions KNAUER Installation Bruises Damage to the device by carrying or lifting it on protruding housing parts The device may fall and thus cause injuries Lift the device on the side of the housing only Clasp the device at its side panels and lift it out of the packag ing Do not hold onto front cover or leak tray Operating Environment Only if the requirements for ambient conditions are met can the intended use be ensured Sunlight Protect the device against direct e
52. off The power plug has been pulled The front cover has been removed Flangeless fitting Device defect The pump head can get damaged when the inlet and outlet are blocked Remove the cap fittings from the inlet and outlet of the pump head prior to use P 6 1L User Manual V6890 Version 1 4 21 Installation Slide the flangeless fit ting onto the tubing Insert the tubing into the free inlet on the bottom of the pump head Tighten the fitting by hand nue Fig 12 Solvent line on the pump head Nextsteps Integrate the pump into the HPLC flow system Connecting the Solvent Selection Valve to the Solvent Note The Solvent Selection valve is readily connected to the 2 chan nel degasser The Solvent Selection valves allows selecting from two different solvents for each solvent channel without having to re install the tubing For both solvent channels A and B one of two sol vents can be selected Solvent A is connected to inlets AT and A2 solvent B is connected to inlets B1 and B2 Prerequisite The device has been switched off The power plug has been pulled The front cover has been removed Material Flangeless fitting 1 Connect the tubing from the four solvent bottles to the inlets A1 A1 A2 and B1 B2 2 Seal inlets not in use with cap fittings 81e Fig 13 Solvent Selection valve with cap fitting Nextsteps Integrate the pump into the HPLC
53. ower Supply Prerequisite The device has been switched off Procedure Process 1 Pull the power plug from the socket and afterwards remove from the device 2 Pack the power cable with the device Next steps Disconnect all remaining electrical connections remove the pump head and pack the device for transport or storage Preparing the Pump Head for Storage Prerequisites The power plug has been pulled Auxiliary material Syringe Flushing solution Device defect Residuals chemicals can damage the pump head if they are not being removed before storage For reuse flush the pump head and fill it with isopropanol Before storage seal the inlets and outlets with cap fittings Procedure Process 1 Fill the flushing solution into a syringe and inject into the capillary at the pump head inlet Leave it for 5 minutes 2 Rinse with water 3 Flush the pump head with isopropanol 4 Seal the inlets and outles with cap fittings Next steps Dismount the pump head Dismounting the Pump Head Prerequisite The pump head has been purged The tubes at the inlet and outlet have been removed The pump head s inlet and outlet have been sealed with cap fittings Tools Allen wrench Open end wrench size 1 4 Open end wrench size 13 KNAUER P 6 1L User Manual V6890 Version 1 4 49 Maintenance and Care Chemical burns Aggressive or toxic solvent residue can irritate the skin Wear protective gloves
54. plan on controlling them sepa rately you can use different ports to avoid interference Therefore the port number for each device must be changed and this same number must be entered into the device configu ration of the chromatography software We recommend to use the same port number for all devices in the same system P 6 1L User Manual V6890 Version 1 4 Legend Display Events Remote Con nector 29 Installation Note The port is set to 10001 at the factory You must use the same numbers in the device configuration of the chromatogra phy software as in the device otherwise the connection fails Process 1 Find out port number and change it on the device 2 Enter the port number in the chromatography software Result The connection is established Pin Header Connectors Remote Connector For receiving start control and error signals from external devices For sending start control and error signals to external devices Events off O on l 0000000 0 dil 1 Fig 26 Remote terminal strip Explanations for Remote Connector Explanation Analog GND Reference point of the voltage at the signal inputs Voltage range 0 5 V scalable Analog in Voltage range 0 10V 10 V according to maximum flow rate Start GND Reference point of the voltage at the signal inputs KNAUER P 6 1L User Manual V6890 Version 1 4 30 Installation Signal Explanation Start OUT TTL output
55. plug on the rear of the device is always accessible so that the device can be disconnected from the power supply Finish the installation and connect the device to the mains In this chapter you find information which are relevant for oper ating the pump Note Prior to switching on the pump you should de aerate it to remove air from capillary and tubing Initial Start Up Use this checklist to determine whether the detector is ready for the initial start up Devices is positioned in the correct location he power plug has been pulled If the device is part of an HPLC system you should also note the following he network connection to the router is established The chromatography software has been installed by KNAUER or a company authorized by KNAUER P 6 1L User Manual V6890 Version 1 4 34 Solvent Back pressure Flow rate Run time Drag Mode Flow on Quick suction process Prerequisite Tool KNAUER Operation The capillary has been connected Pump Head To bring a newly installed pump head into service securely it should run in prior to its first operation For this purpose set the following parameters Capillary with inner diameter Capillary with inner diameter of 0 1 mm of 0 1 mm length 2 m length 1 m Back pressures higher than 8 MPa are generated by back pres sure regulators De Aerating the Pump Before the pump can be used it must be de aerated The pump
56. pply to connect the router to the mains power system Configuring the Router The router is preset at the factory You will find a label at the bottom side of the router on which IP address user name and password are printed These information help to open the router configuration Process 1 To open the router configuration start your Internet browser and enter the IP address not for all routers 2 Enter user name and password 3 Configure the router as DHCP server 4 In the router configuration check the IP address range and make changes if necessary Result Once the router has assigned IP addresses to all devices the chromatography software can be used to remotely control the system Integrating the LAN into a Company Network A network administrator can integrate the LAN into your com pany network In this case you use the WAN port of the router Prerequisite There is a patch cable for the connection Process 1 Check that the IP address range of the router and of the com pany network do not overlap 2 In case of an overlap change the IP address range of the router 3 Use the patch cable to connect the router WAN port to the company network 4 Restart all devices including the computer Controlling Several Systems Separately in a LAN Devices connected to a LAN communicate through ports which are part of the IP address If more than one HPLC system is con nected to the same LAN and you
57. rk areas are always well ventilated When performing maintenance tasks on the device always wear safety glasses with side protection protective gloves and a lab coat All wetter components of a device e g flow cells of detectors or pump heads and pressure sensors for pumps have to be flushed with isopropanol first and water afterwards before being main tained disassembled or disposed Opening the device The device may only be opened by the KNAUER Technical Sup port or any company authorized by KNAUER Electronic defect Performing maintenance tasks on a switched on device can cause damage to the device Switch off the device Pull the power plug Device defect Leaks can damage the device If leaks occur after maintenance or assembly replace the cap illary connections with new ones Users may perform the following maintenance tasks themselves Replacing the pump head Replacing the check valves of the pumps KNAUER P 6 1L User Manual V6890 Version 1 4 45 European hotline E mail contact Operating hours KNAUER Maintenance and Care Replacing the Inline filter of the pressure sensor Replacing the Mixer Proper maintenance of your HPLC device will ensure successful analyses and reproducible results Contact with the Technical Support If you have any technical questions regarding KNAUER hardware or software please use one of the contact options below Technical Support hotline
58. s Isocratic HPLC pump Quaternary low pressure gradient pump Binary high pressure gradient pump Pulsation com Active pressure and pulsation compensa pensation tion Pulsation 2 96 amplitude typically 1 3 96 or 0 3 MPa 3 bar whatever is greater at 1 ml min ethanol at all pressures 1 MPa 10 bar 147 psi 0 001 10 ml min 0 02 10 ml min recommended 0 01 50 ml min 0 1 40 ml min recommended Flow rate range Flow rate 0 001 ml min increment Flow rate 1 96 measured at 5 80 96 of flow accuracy range using ethanol Flow rate 0 1 96 RSD based on retention time at precision constant room temperature Piston seal wash Standard ing System protection Soft start P and Pmax are programma ble KNAUER P 6 1L User Manual V6890 Version 1 4 58 Technical Data Wetted materials Stainless steel graphite fiber reinforced PTFE FKM PEEK sapphire aluminium oxide Al203 Degasser module Degasser 2 channels Teflon AF channels Degasser maxi 10 ml min mum flow rate Degasser method Gas permeation through Teflon AF amorphous fluoropolymer membrane Degasser lt 0 5 ppm dissolved O gt at 1 ml min efficiency Degassing 480 ul volume per channel chamber volume Solvent applica Universal with the exception of hydro bility chloric acid and halogenated hydrocar bons in particular hexafluoroisopropanol HFIP Wetted materials PEEK Tefzel Teflon AF Vacuum cham
59. s of components caused by overtightened fittings Pay attentio to the torque values Use maximum 5 Nm for stainless steel fittings Use maximum 1 Nm or finger tight and an additional quarter of a turn with appropriate pliers for PEEK fittings KNAUER P 6 1L User Manual V6890 Version 1 4 52 Maintenance and Care Procedure Process 1 Insert the check valves in such a way that the notch of the check valve Q points down ward Screw in inlet and out let fittings and tighten them with a torque Fig 35 Check valve wrench and the respec tive torque Next steps Re install the pump head Replacing the Inline Filter Clogged inline filters inside of the pressure sensor can cause pressure fluctuations and irregular flow Inline filters are not cleaned but exchanged as an assembly Procedure 1 Remove the capillary below the inline filter of the pressure sensor 2 Loosen the fitting of the inline filter and remove manually 3 Insert a new inline filter cartridge Removing the Inline Filter Below the pressure sensor you find the fitting of the inline filter Prerequisite The pump has been rinsed Tools Open end wrench size 1 4 Open end wrench size 13 Procedure Process 1 Fixate the outlet fitting with an open end wrench size 13 Loosen the fitting below the inline filter of the pressure sensor with the open end wrench size 1 4 p gt vi X SS K l Fig 3
60. sol vent to escape it is prohibited to have any open flames near the analytical system solvent tray Risk of electrical shock or short circuit if liquids get into the device s interior For this reason place all bottles In a solvent tray Solvent lines Install capillaries and tubing in such a way that liquids cannot get into the interior in case of a leak leaks Regularly check if any system components are leaking power cable Defective power cables are not to be used to connect the device and the power supply system self ignition point Only use eluents that have a self ignition point higher than 150 C under normal ambient conditions P 6 1L User Manual V6890 Version 1 4 13 Safety for Users power strip If several devices are connected to one power strip always consider the maximum power consumption of each device power supply Only connect devices to voltage sources whose voltage equals the device s voltage toxicity Organic eluents are toxic above a certain concentra tion Ensure that work areas are always well ventilated Wear protective gloves and safety glasses when working on the device Where is use of the device prohibited Never use the system in potentially explosive atmospheres with out appropriate protective equipment For further information contact the Technical Support of KNAUER Decommissioning the Device Securely At any time take the device completely out of operation by e
61. start up 33 P 6 1L User Manual V6890 Version 1 4 66 Inline filter 45 52 accessories and spare parts 60 Insert 53 remove 52 replace 52 IO 37 Isocratic 64 Isocratic pump 6 delay volume 55 L LAN 26 64 port 29 problems 38 router 28 settings 27 setup 27 troubleshooting 38 Leak remove 54 leak 12 Leak management connect 25 Leak sensor 54 LED 36 64 Low pressure side 64 LPG pump 6 7 18 20 22 64 M Maintenance maintenance contract 45 maintenance intervals 45 Maximum pressure 57 Mixer 9 45 64 accessories and spare parts 60 replace 54 technical data 56 Mixing volume binary 56 quaternary 56 Mobile Control 9 36 45 64 Modifiers 9 O OpenLAB 9 36 45 Operating hours 45 OQ 37 D Packing list 17 KNAUER Index PEEK 10 Acetonitrile 18 pH 64 Pin header 29 wire 32 Piston backflushing 9 11 49 flushing solution 11 Piston backflusing connect 24 PMax Mode 9 port LAN 29 power strip 13 supply 13 Power consumption 15 59 Power supply connect 33 power supply cable 12 Pressure sensor 6 7 47 64 Problems and rectifications 39 professional group 11 Pump 64 binary 6 18 20 de aerate 34 flush 46 HPG 6 integrate into a system 18 isocratic 6 18 LPG 6 7 18 20 22 put out of operation 47 quaternary 6 7 22 switch on 35 Pump head 55 accessories and spare parts 60 connect to solvent 19 20 22 dismount 48 run in 34 storage 48 Pump head cooler
62. tact for at least 1000 ms Permissible load of the relay contact 1 A 24 V DC Wiring the Terminal Strip Electronic defect Electrostatic discharge can destroy the electronics Wear a protective bracelet against electrostatic discharge and ground KNAUER P 6 1L User Manual V6890 Version 1 4 33 Prerequisites Power cable Power plug Next steps Operation KNAUER Operation Analog Control Analog ports serve for exchanging analog control signals Refer ence point for the signals is the connector GND OUT Device sends signal IN Device receives signal Power Supply Note The nominal capacity of the connected devices must be maximum 50 of the power supply to account for larger inrush currents when switching on the modules The maximum power input is 100 watts The electrical power supply at the installation site must be connected directly to the nearest main power line The power must be free from ripple residual current voltage peaks and electromagnetic interference The connectors for the mains voltage are grounded accord ingly The device receives sufficient power with reserve capacity The device is intended for use with AC power networks of 100 240 V Use only the enclosed power cable to connect the device to the mains Replace defective power cables only with accessories from KNAUER Only use power cables admitted for use in your country Make sure that the power
63. ted Process 1 In Windows 7 choose Start Control Panel Network and Sharing Center Double click on LAN Connection Click on the button Properties Select Internet Protocol version 4 TCP IPv4 Click on the button Properties NM BR W N Check the settings in the tab General The correct settings for the DHCP client are a Obtain IP address automatically b Obtain DNS server address automatically 7 Click on the button OK Connecting the Cables A router has several LAN ports and one WAN port that can be used to integrate the LAN into a wide area network WAN e g a company network or the Internet In contrast the LAN ports serve to set up a network from devices and a com puter To avoid interference we recommend operating the HPLC system separately from the company network D 4 pT e LAN RE GS Gi GS IG m RE unl Internet You will find patch cables for each device and the router in the accessories kit To connect the router to a WAN an additional patch cable is required which is not supplied within the scope of delivery Prerequisite The computer has been switched off P 6 1L User Manual V6890 Version 1 4 28 KNAUER Installation There is a patch cable for each device and the computer Process 1 Use the patch cable to connect the router and the computer Repeat this step to connect all devices 2 Use the power su
64. tion of a solvent remains constant Local area network LAN Network in which HPLC devices and a computer are connected in order to control the devices LED Light emitting diode Low pres Devices and capillary which are under high sure side pressure in an HPLC system LPG pump Pump configured to generate a gradient on the low pressure side Only one pump unit is used which contains small doses of the different sol vents The solvents are mixed in small mixing cycles by a valve block which is connected upstream The gradient is generated before the pump Mixer Component for the homogeneous mixing of solvents for the generation of a gradient Mobile Mobile application to control and program the Control whole HPLC system via wireless LAN pH Measure for the acidic or alkaline character of an aqueous solution Pressure Component for measuring the system pressure sensor Pump Device which conveys solvents in controlled quantities into a system Quaternary Pump configured to generate a gradient from pump four solvents Solvent Flowing agent that transports the substances to be separated or isolated through the column eluent mobile phase Component of the pump that allows selecting Solvent Selection valve from two different solvents for every solvent channel With this mechanism the solvent can be changed without having to manually change the system Device for varying the solvent flow KNAUER P 6 1L User
65. tschland Pump P 6 1L Product no EPH30 EPH34 EPH35 EPH38 EPH39 EPH60 EPH64 EPH65 EPH68 EPH69 The product complies with the following standards Machinery Directive 2006 42 EC EMC Directive 2004 108 EC IEC 61000 3 2 2012 IEC 61326 1 2011 ROHS Directive 2011 65 EU WEEE Directive 2012 19 EU Low Voltage Directive 2006 95 EC IEC 61010 1 2010 IEC 61010 2 081 2001 A1 Canada CSA C22 2 No 61010 1 3rd Edition 2012 USA UL 61010 1 3rd Edition 2012 The product was tested with a typical configuration The mark of conformity has been applied to the rear panel Berlin 24 04 2015 JB nas Alexanda Knauer CEO and owner part of V6890 KNAUER Wissenschaftliche Gerate GmbH All rights reserved The information in this document is subject to change without prior notice Translation of the original German edition of this manual version 1 4 2015 09 02 Printed in Germany on environmentally friendly paper from sustainable forests AZURA are registered trademarks of KNAUER Wissenschaftliche Gerate GmbH www knauer net downloads See up to date manuals online www knauer net HPLC SMB Osmometry KNAUER Phone 49 30 809727 0 Wissenschaftliche Ger te GmbH Telefax 49 30 8015010 Hegauer Weg 38 E Mail info knauer net a a P ANM gt 14163 Berlin Germany Internet www knauer net KNAUER 2015 V6890 0 01 05 14 Mimeo
66. twork Is LAN DHCP set for controlling Did the device receive an IP address P 6 1L User Manual V6890 Version 1 4 39 KNAUER Troubleshooting 6 Turn off all devices router and computer Firstly turn on the router and secondly turn on the devices and the computer Has this been successful 7 Replace the patch cable to the device with that no connection could be established Has this been successful 8 Make sure that the IP port of the device matches the port in the chromatography software Possible Problems and Troubleshoot ing tror aan MN Device cannot be Inspect the power cable to ensure that it switched on is plugged into the power supply When purging the Check if the venting screw on the pres pump switches off sure sensor is turned up Pump does not Purge the pump head to remove the transport solvent air bubbles Clean the check valves Exchange the check valves If the pump head seals are defective solvent enters the piston backflush ing inform the technical support of the manufacturer Exchange the pump head Pressure and flow Purge the pump head to remove rate variations excess air Always tighten the inlet screw fittings and outlet screw fittings on the pump head with a torque wrench 5 Nm torque for a stainless steel pump head 3 5 Nm torque for a ceramic pump head Clean the check valves Exchange the check valves Exchange the pump head Contact the T
67. xposure to sun light AC system Set up the device at a location not exposed to air drafts Vibration Do not set up the device in the vicinity of other machines that cause floor vibrations Installation site Position the device on a level and even surface Height above sea level maximum 2000 m Weight 11 5 kg isocratic 14 1 kg binary 12 7 kg quaternary Dimensions 361 mm x 208 2 mm x 523 mm width x height x depth Power supply Input 100 240 V Output 50 60 Hz Maximum power 100 Watt consumption Humidity lt 90 non condensing Temperature 4 40 C 39 2 104 F It is possible to operate the pump in a cooling compartment when the following requirements are fulfilled the pump is switched on the pump is on standby mode Device defect Changes of the environmental temperature cause condensation inside the device Allow device to acclimate for 3 h before connecting to power supply and taken into operation P 6 1L User Manual V6890 Version 1 4 16 Earth quake regions Prerequisite Tool Procedure KNAUER Installation If the device is set up in a region where earth quakes are com mon secure the device at the two fixing points The fixing points are situated on both sides of the device Fig 6 Fixing points on the device Setup Prior to setting up the device find a suitable place according to the requirements and remove the packaging The requirem

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