FLoid® Cell Imaging Station
Contents
1. Q IMPORTANT To avoid electrical shock always turn off the FLoid Cell Imaging Station and unplug the power cord before cleaning or decontaminating the instrument N WARNING Using a cleaning or decontaminating method other than that specified by the manufacturer may result in damage to the instrument N WARNING Biological samples such as tissues body fluids and blood of humans and other animals have the potential to transmit infectious diseases Follow all applicable local state provincial and or national regulations Wear appropriate protective eyewear clothing and gloves e Wipe the LCD monitor of the FLoid Cell Imaging Station using a soft lint free cloth moistened with an LCD cleaning solution Do not apply excessive force during cleaning Wipe the LCD dry immediately after cleaning e Ensure that the cleaning solution does not enter the power switch the power inlet or the USB ports e Never pour or spray any liquids directly on the instrument to avoid electrical shock when the instrument is plugged in e Donot use abrasive cleaning solutions or material to prevent the touch screen from getting scratched e Wipe the instrument exterior using a soft lint free cloth moistened with distilled water Wipe the instrument dry immediately after cleaning e Ensure that water or other cleaning solutions do not enter the power switch the power inlet or the USB ports e Never pour or spray any l2. Date Networking Copy Error Logs ire Please place a cover over the lens so that no stray light enters the No 13422 microscope arial No 00000000000 Default Printer a a e NNNM Canon SELPHY CP800 Paper Size 4 x6 100x148mm Postcard Note The Hot Pixel Correction only needs to be performed once per v month The device retains pixel correction information through ON OFF power cycles Update Software The software updates to FLoid Cell Imaging Station the can be found by registering your device or by checking the FLoid Cell Imaging Station website at www lifetechnologies com FLoid 1 Select the Settings Tab to display the Settings Screen 2 Insert a USB memory device containing the software update file from the FLoid Cell Imaging Station webpage 3 Click the Update button located next to the current version number of the software 4 Follow the onscreen commands bt Note Once the software has been updated the device will retain this b information through ON OFF power cycles Continued on next page 22 Getting Started continued Optional The FLoid Cell Imaging Station can be connected to a network to allow data Networking files to be directly saved to a network drive 1 Select the Settings Tab to display the Settings Screen 2 Select Open Settings to open the Network Settings pop up window Networking Status Network Status Adapter Not Ready Mapped Drive Not conne
3. Slider physically adjusts the LED excitation light intensity camera gain and exposure time The displayed percentage corresponds each time to the same LED excitation light intensity camera gain and exposure time Light Bulb Icons Turns the LED excitation light sources ON and OFF Image Thumbnails Displays small images of captured color channels The image can be deleted by clicking e located on the top right of the image thus enabling the capture of a new image Text Box Allows the assignment of a user defined name for the color channel Click and edit the text to give a customized name to the color channel Capture Button Captures a static picture of the live feed from the camera Capturing Processing Toggle Switches between the Capturing and Processing screens of the Image Acquisition Tab Continued on next page Image Acquisition Tab continued Processing Screen The Processing screen contains the controls for processing the captured images of your sample g Molecular Probes Reagents K Image Acquisition Image Review Zoom Scale Brightness Slider Contrast Slider Save Button Color Selector Scale Bar Print Button e Zoom Scale Allows digital zoom of captured images and can be adjusted between 80 and 400 in increments of 1 e Scale Bar Used for estimating the size of objects in micrometers um Clicking QJ on the right side of the scale bar hides it from view clicking Show reveals it The s
4. Submit a question directly to Technical Support techsupport lifetech com e Search for user documents SDSs vector maps and sequences application notes formulations handbooks certificates of analysis citations and other product support documents e Obtain information about customer training e Download software updates and patches Safety Data Sheets SDSs are available at www lifetechnologies com sds Life Technologies Corporation and or its affiliate s warrant their products as set forth in the Life Technologies General Terms and Conditions of Sale found on Life Technologies website at www lifetechnologies com termsandconditions If you have any questions please contact Life Technologies at www lifetechnologies com support 45 Headquarters 5791 Van Allen Way Carlsbad CA 92008 USA Phone 1 760 603 7200 Toll Free in USA 800 955 6288 For support visit lifetechnologies com support or email techsupport dlifetech com lifetechnologies com technologies 3 December 2012
5. a hazard symbol These hazard symbols are identical to the hazard symbols that are affixed to Life Technologies instruments see Symbols on page 37 The Safety Data Sheets SDSs for any chemicals supplied by Applied Biosystems are available to you free 24 hours a day For instructions on obtaining SDSs see SDSs on page 44 Q IMPORTANT For the SDSs of chemicals not distributed by Life Technologies contact the chemical manufacturer Product Information Product Contents What s in the Box Upon Receiving the Instrument Registering Your Instrument Product Use The FLoid Cell Imaging Station is shipped with the components listed below Component Quantity FLoid Cell Imaging Station each Keyboard 1 each Mouse each Universal Power Cord with 4 adaptor plugs each for U S E U U K Australia USB Drive includes the user manual each Quick Start Guide each Certificate of Conformity CoC each See pages 7 19 for Product Description and page 32 for FLoid Cell Imaging Station Product Specifications Examine the instrument carefully for damage incurred during transit Ensure that all parts of the instrument including accessories listed above are included with the product Damage claims must be filed with the carrier the warranty does not cover in transit damage See page 20 for Getting Started Visit www lifetechnologies com FLoid to register your instrument You will be
6. asked to supply the serial number your name and your contact details Registering your instrument ensures that you will receive notifications of Software Upgrades and information on new reagents and assays for use with the FLoid Cell Imaging Station For Research Use Only Not for use in diagnostic procedures Description of the FLoid Cell Imaging Station FLoid Cell Imaging The FLoid Cell Imaging Station is a benchtop device designed to give quick Station access to fluorescence microscopy It has an intuitive design for easy operation Images are focused manually via coarse and fine adjustment knobs but most other features are accessed through the user interface The FLoid Cell Imaging Station is a fully integrated system combining microscope optics electronics and mechanics with an onboard computer and a widescreen LCD monitor The FLoid Cell Imaging Station features three fluorescence color channels in addition to a relief phase contrast channel The blue green and red fluorescent channels are stationary and are not user changeable They correspond to the wavelengths of the most commonly used dyes DAPI FITC and Texas Red The sample stage was designed to give maximal sample vessel flexibility Everything from microscope slides to multiwell plates can be imaged on the FLoid Cell Imaging Station Sample vessel movements are performed manually The stage offers a few millimeters of lateral motion for smooth viewing
7. designed for connecting the keyboard mouse and the optional printer to the FLoid Cell Imaging Station Indicator Light The indicator light indicates that the instrument is turned ON If no indicator light is present then the device is turned OFF or unplugged from the power outlet Objective Opening The objective opening of the FLoid Cell Imaging Station is located at the center of the stage Because the light is emitted from the objective opening during image acquisition the sample to be imaged should cover the objective opening Power Inlet The power inlet connects the instrument to an electrical outlet through the supplied power cord and the appropriate plug based on the electrical outlet configuration in your country Power Button The power button is used for turning the device on and off Focus Knobs The focus knobs are located on both sides of the instrument and each set contains a larger coarse focus knob and a smaller fine focus knob The knobs are used for focusing the image of the sample Light Shield The light shield pivots up for easy sample loading onto the stage The shield also blocks overhead ambient light from interfering with image acquisition FLoid Cell Imaging Station User Interface Overview of the When the FLoid Cell Imaging Station is turned on two loading screens appear Graphical User briefly which then give way to the Graphical User Interface GUI Some of the Interface GUI features of t
8. detailed protocols see the Molecular Probes Reagents Tab on the user interface of the device For your convenience assay and protocol information can be placed directly on a smartphone or tablet PC device by downloading the Cell Imaging application found in the App Store or Android Market This user guide is for laboratory staff operating maintaining and analyzing data using the FLoid Cell Imaging Station Two user attention words appear in Life Technologies user documentation Each word implies a particular level of observation or action as described below Note Provides information that may be of interest or help but is not critical to the use of the product IMPORTANT Provides information that is necessary for proper instrument operation accurate installation or safe use of a chemical CAUTION Indicates a potentially hazardous situation that if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices WARNING Indicates a potentially hazardous situation that if not avoided could result in death or serious injury DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious injury This signal word is to be limited to the most extreme situations PPP Except for IMPORTANT safety alerts each safety alert word in a Life Technologies document appears with an open triangle figure that contains
9. of samples The user interface aids in intuitive operation and requires minimal to no previous microscopy experience to operate The interface features thumbnails of collected images icons for quick recognition and reagent information including scientific protocols The reagent information and protocols cover about 160 different reagents in 20 application categories All the information is presented with quick reference icons and concise text for maximal usability A unique feature of FLoid Cell Imaging Station is the Focus Assist gauge found on the user interface This scale is designed to help aid in rapid focusing by tracking the position of the objective lens and outputting a number This number can be correlated to a particular sample vessel to more quickly focus on a sample in the future See page 9 for details on various parts of the FLoid Cell Imaging Station Continued on next page Description of the FLoid Cell Imaging Station continued Features of the Important features of the FLoid Cell Imaging Station are FLoid Cell Imaging Station Fully integrated system for multicolor fluorescence imaging in less than a minute Molecular Probes reagent and protocol information built into the user interface for quick reference Light shield which blocks ambient light and enables imaging at the bench or tissue culture facility in normal ambient room lighting conditions Open stage to accommodate most any type of
10. protocol Reagent Details J Molecular Probes Reagents K3 Image Acquisition 3 Image Review Anti Mouse Protocol Alexa Fluor Antibodies Hands on time Bs Total time D Sample FIXED Amount Goat anti mouse 488 0 5 mL 6 Donkey anti mouse 488 0 5 mL 6 Goat anti mouse 594 0 5mL Alexa Fluor 488 or Alexa Fluor 594 labeled goat or Donkey anti mouse 594 05mL donkey anti mouse IgG heavy and light chain highly cross adsorbed secondary antibodies Goat anti mouse 488 A11029 Concentration Donkey anti mouse 488 A21202 Goat anti mouse 488 2 mg mL Goat anti mouse 594 A A11032 Donkey anti mouse 488 2 mg mL Donkey anti mouse 594 A21203 Goat anti mouse 594 2 mg mL Reagent Details contains the following information about the selected reagent Brand Name Provides the brand name of the reagent if available Typical Result Displays an image highlighting the type of result possible when using the reagent Product Name Provides an abbreviated version of complete product name Emission color Represented by a large colored circle indicates the emission color of the dye s in the selected product Description Provides a brief description of the product Hands on time Indicates the time in minutes unless an h for hours is present that it takes to actively perform the protocol using the selected reagent Total Time Indicates the time in minutes unless an h for hours is present that it takes to
11. underlying channels allows you to save each of the color channels as an unaltered black amp white TIFF file Each color channel is then appended with the text description under the thumbnail e Save function stores the image as seen in the image pane Optional Print e To print an image as seen in the image pane click on the Print icon Image e You can select the default printer and the paper size from the Settings screen Printing Default Printer Canon SELPHY CP800 v Paper Size 4 x6 100x148mm Postcard v 30 Software Upgrades Updating the Software The update feature allows you to update the FLoid Cell Imaging Station when new software versions are available This ensures the optimal performance of the instrument 1 Download the latest software to your USB drive from our website at www lifetechnologies com FLoid 2 Plugthe USB drive containing the latest software version into the FLoid Cell Imaging Station 3 Click Update on the Settings Screen and follow the on screen instructions Software Version No 13422 The software will be updated 31 Cleaning the FLoid Cell Imaging Station Introduction Cleaning the LCD Monitor Cleaning the Instrument Exterior Decontaminating the Instrument Although the FLoid Cell Imaging Station requires little maintenance for proper performance we recommend that the instrument be cleaned periodically to prevent the buildup of dust and dirt
12. Brightness and contrast adjustments are digital enhancements and do not change the original image data e Each color channel can be adjusted separately by selecting one of the four color swatches surrounding the black white toggle p e Brightness and Contrast sliders change color to correspond to the color channel being adjusted The example below shows the Brightness slider with the green channel selected e Color channels can be turned off by clicking on the green check mark on the image thumbnail e Brightness and Contrast adjustments are performed for each color channel separately e Color channels can be displayed or hidden by selecting thumbnails e Green check mark denotes the displayed color channels in the composite view Continued on next page 29 Using the FLoid Cell Imaging Station continued Save Image e To permanently store an image as seen in the image pane click on the USB Save icon which opens the Save dialog box CE WR Presentations fluocells 1 Blue Light tif fluocells 1 Green Light tif fluocelis 1 Red Light tif fluocells 1 jpg no550 control raw Blue Light tif no550 control raw Green Light tif no550 control raw jpg no550 ifnlps raw Blue Light tif iv File name File format JPEG jpg Ww o save underlying channels e Enter a file name and select a file type from the drop down menu Available file types are JPEG TIFF BMP and PNG file formats e Clicking save
13. DSs provide the safety information you need to store handle transport and dispose of the chemicals safely Each time you receive a new SDS packaged with a hazardous chemical be sure to replace the appropriate SDS in your files You can obtain from Life Technologies the SDS for any chemical supplied by Life Technologies This service is free and available 24 hours a day To obtain SDSs 1 Goto www lifetechnologies com click Support and then select SDS 2 Inthe Keyword Search field enter the chemical name product name SDS part number or other information that appears in the SDS of interest Select the language of your choice and then click Search 3 Find the document of interest right click the document title then select any of the following e Open To view the document e Print Target To print the document e Save Target As To download a PDF version of the document to a destination that you choose IMPORTANT For the SDSs of chemicals not distributed by Life Technologies contact the chemical manufacturer 44 Documentation and Support Obtaining Support Technical Support Safety Data Sheets SDS Limited Product Warranty For the latest services and support information for all locations go to www lifetechnologies com At the website you can e Access worldwide telephone and fax numbers to contact Technical Support and Sales facilities e Search through frequently asked questions FAQs e
14. Loid Cell Imaging Station is compatible for use with most mammalian cell culture vessels including microscope slides and multi well plates Use thin glass bottom sample vessels for best optical clarity e g Matek dishes or microscope slides Image through phenol red free medium without serum for least amount of background Use dyes with spectrally similar wavelengths to DAPI FITC and Texas Red e g Hoechst Alexa Fluor 488 and Alexa Fluor 594 Wear gloves and other appropriate personal protective equipment when handling samples Place the instrument on a flat dry surface that is free from excessive vibration Do not spill liquids on the surface of the instrument or introduce liquids into its interior We recommend that you turn off the FLoid Cell Imaging Station at the end of the day Do not attempt to open the device The FLoid Cell Imaging Station has a single power switch located on the right side of the device Press the power switch and allow the device to start up The start up procedure takes approximately 60 seconds Indicator p Light i ON OFF Switch Continued on next page 24 Using the FLoid Cell Imaging Station continued Place the Sample on the Stage Select Color Channel The part of the FLoid Cell Imaging Station where the samples are placed is called the stage The stage has a single objective lens opening in its center and the opti
15. US R GUIDE molecular probes by Kafe technologies FLoid Cell Imaging Station Catalog Number 4471136 Document Part Number MP44711 Publication Number MANOOO05351 Revision 2 0 9 technologies For Research Use Only Not for use in diagnostic procedures Information in this document is subject to change without notice DISCLAIMER LIFE TECHNOLOGIES CORPORATION AND OR ITS AFFILIATE S DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE OR NON INFRINGEMENT TO THE EXTENT ALLOWED BY LAW IN NO EVENT SHALL LIFE TECHNOLOGIES AND OR ITS AFFILIATE S BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING BUT NOT LIMITED TO THE USE THEREOF NOTICE TO PURCHASER LIMITED USE LABEL LICENSE Research Use Only The purchase of this product conveys to the purchaser the limited non transferable right to use the purchased amount of the product only to perform internal research for the sole benefit of the purchaser No right to resell this product or any of its components Is conveyed expressly by implication or by estoppel This product is for internal research purposes only and is not for use in commercial applications of any kind including without limi
16. X optical 1840X with digital zoom Fluorescence and transmitted light relief phase 4 channels relief phase blue fluorescence green fluorescence red fluorescence LED 50 000 hour life adjustable intensity Blue channel 390 40 nm Green channel 482 18 nm Red channel 586 15 nm Blue channel 446 33 nm Green channel 532 59 nm Red channel 646 68 nm 60 mm Sony 1 3MP 1 3 ICX445 EXview HAD CCD Mechanical glide stage range of motion 10 mm in X and Y dimensions 15 inch color display resolution 1366 x 768 pixels Image resolution 1296 x 964 pixels adjustable tilt FLoid Cell Imaging Station Software www lifetechnologies com FLoid 16 bit monochochrome TIFF PNG BMP and JPG 1296 x 964 pixels 2 Gigabyte 35 Appendix B Safety Safety Information Safety Precautions Review and follow the safety instructions below Do not install the instrument in heavy humidity such as a greenhouse or an incubator to avoid a danger of electric shock If water or other material enters the instrument the adaptor or power inlet disconnect the power cord and contact a service person For operating environment refer to Product Specifications page 35 Do not touch the main plug or power cord with wet hands Always ensure that the power supply input voltage matches the voltage available in your location This instrument is air cooled so its surfaces may become hot during operation When installing the instru
17. accessories Light Ss NON Shield SX Nw SS aan Sample WC f Plug the instrument to an Stage approved outlet Objective ON OFF Opening Turn on the instrument The Switch FLoid Cell Imaging Station is now ready for use Focus Knob Place the sample on stage over the objective opening Focus the image using the focus knobs Capture the image using the on screen controls Image Pane Light Switch OFF g Molecular Probes Reagents Image Review Light Switch ON yr Text Box Image Thumbnails Capture Button Light Intensity Slider Black White Toggle Capturing Processing Toggle Adjust the Brightness and Contrast as needed Save Button Zoom Scale Print Button Contrast Slider Brightness Slider Color Selector Scale Bar Save the image to the USB Memory Drive Optional Print the image via the USB Printer Preface About this Guide Purpose of this User Guide Audience User Attention Words SDSs The FLoid Cell Imaging Station User Guide is designed to help you learn how to use the instrument The user guide includes step by step instructions for instrument set up image capture and image processing it does not contain assay specific protocols The complete user guide is also available on our website at www lifetechnologies com For information on specific assays for the FLoid Cell Imaging Station including
18. alation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For additional safety guidelines consult the SDS Handle chemical wastes in a fume hood After emptying the waste container seal it with the cap provided Dispose of the contents of the waste tray and waste bottle in accordance with good laboratory practices and local state provincial or national environmental and health regulations Waste Disposal If potentially hazardous waste is generated when you operate the instrument you must Characterize by analysis if necessary the waste generated by the particular applications reagents and substrates used in your laboratory Ensure the health and safety of all personnel in your laboratory Ensure that the instrument waste is stored transferred transported and disposed of according to all local state provincial and or national regulations IMPORTANT Radioactive or biohazardous materials may require special handling and disposal limitations may apply 40 Electrical Safety Fuses Power AN DANGER ELECTRICAL SHOCK HAZARD Severe electrical shock can result from operating the FLoid Cell Imaging Station without its instrument panels in place Do not remove instrument panels High voltage contacts are exposed when instrument panels are removed from the instrument WARNING FIRE HAZARD For continued protection against the risk
19. aterials needed to perform the experimental protocol but not supplied with the product e Protocol Icons Provide a graphical representation of the steps in the protocol e Experimental Protocol Provides a step by step description of the experimental protocol Note For more information on dyes and reagents to use with the y FLoid Cell Imaging Station download the Cell Imaging smartphone app in the App Store or Android Market 16 Image Review Tab Image Review Tab The Image Review Tab is used to acces saved images and allows you to Review previously saved images Organize images into folders Create new folders Delete images View saved files J Molecular Probes Reagents Ko Image Acquisition WR Image Review 1 DA Dx fab Presentations fluocells 1 Blue Light tif fluocells 1 Green Light tif fluocells 1 Red Light tif fluocells 1 jpg no550 control raw Blue no550 control raw Green gt no550 ifnlps raw Blue no550 ifnlps raw Green no550 control raw jpg d i Light tif Light tif Light tif Light tif The Image Review Tab contains the following Address bar Describes the file location Up Folder button Navigates up to a higher level folder New Folder button Creates a new folder Folders Are used for storing similar files Thumbnails Display previews of stored image files List Thumbnail toggle E Switches between the List view an
20. cable Safety Data Sheets SDSs See SDSs on page 44 WARNING Visible and or invisible LED radiation Do not stare directly into the beam or view directly with optical instruments CAUTION Using cleaning or decontamination methods other than those recommended by the manufacturer may compromise the safety or quality of the instrument 38 Chemical Safety Chemical Hazard WARNING CHEMICAL HAZARD Before handling any chemicals Warning refer to the Safety Data Sheet SDS provided by the manufacturer and observe all relevant precautions AN WARNING CHEMICAL STORAGE HAZARD Never collect or store waste in a glass container because of the risk of breaking or shattering Reagent and waste bottles can crack and leak Each waste bottle should be secured in a low density polyethylene safety container with the cover fastened and the handles locked in the upright position Wear appropriate eyewear clothing and gloves when handling reagent and waste bottles General Safety To minimize the hazards of chemicals Guidelines e Read and understand the Safety Data Sheets SDSs provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials See SDSs page 44 e Minimize contact with chemicals Wear appropriate personal protective equipment when handling chemicals for example safety glasses gloves or protective clothing For additional safety guidelines c
21. cale bar value automatically adjusts between 25 um and 125 um according to value chosen on the Zoom Scale e Brightness Slider Controls the image brightness for a single color channel of a captured image Brightness can be adjusted between 0 and 100 in increments of 1 e Color Selector Selects the color channel to be adjusted by the Brightness and Contrast Sliders e Contrast Slider Controls image contrast for a single color channel of a captured image Contrast can be adjusted between 0 and 100 in increments of 1 e Image Thumbnails Display small images of captured color channels Images can be shown in the Image Pane by clicking on the grey check mark or removed by clicking on the green check mark e Save Button Brings up a Save Dialog Box for storing the Image Pane view to a USB storage device Available file formats are PNG TIFF JPEG and BMP Only the TIFF file format allows for the underlying layers or color channels to be saved e Print Button Brings up a Print Dialog Box for printing the Image Pane view 13 Molecular Probes Reagents Tab Molecular Probes Reagents Tab Molecular Probes Reagents Tab provides a reagent selection guide with respective protocols It contains information over 150 commonly used Molecular Probes reagents that are organized by application area Information about each reagent includes technical specifications and an image showing typical results Application Icons Rea
22. cell culture vessels Long life LED light sources for safe and reliable performance Built in optical filters Fluorite objective lens for sensitive detection of weakly fluorescent samples Focus Assist to aid in rapid focusing of images Fine and coarse focus knobs on left and right sides of device 15 inch widescreen LCD with tilting function Front facing USB port for hassle free file saving Intuitive user interface for quick operation Image processing built into acquisition interface Thumbnail confirmation of captured images On screen digital zoom On demand scale bar for size determination Compact laptop style keyboard and wireless mouse Continued on next page Description of the FLoid Cell Imaging Station continued Parts of the FLoid Cell Imaging Station LCD Light Shield Indicator Light Objective Opening Stage USB Ports USB Port B Focus Knobs On Off Switch Stage Movement Knobs LCD The LCD liquid crystal display shows the user interface which contains the settings used to control the device Stage The stage is where sample vessels such as cell culture dishes and microscope slides are placed for imaging Stage Movement Knobs The stage movement knobs allow the stage to be moved in the X Y plane USB Ports The front facing USB port is designed for daily easy access to transfer images from the FLoid Cell Imaging Station to a USB memory device The side facing USB ports are
23. cs of the FLoid Cell Imaging Station is located directly below the objective lens opening Therefore the portion of the sample to be imaged needs to be placed over the objective lens opening in the stage Light Shield Stage Objective Opening Focus Knob e Place samples on the stage directly over the objective lens opening e Becareful not to spill liquids in the objective lens opening e Lift the light shield for easier sample placement and to see which well of a multi well plate is being imaged e lifting the light shield turns off the fluorescent LEDs The light bulb symbols on the right panel of the user interface control the excitation LEDs Select the appropriate LED based on the emission color of the dye in the sample For example a sample labeled with FITC Alexa Fluor 488 or GFP have green emission therefore select Green Light for this sample e Select the color channel based on dye emission wavelength e Light sources are LEDs and do not require a warm up time e Lights can be switched on and off as needed Note that the fluorescent LEDs will not activate when the light shield is raised e After capturing an image see page 28 in a particular channel you can click to delete and re capture an image e Color channels can be personalized with a text description Text descriptions appear in the file name if save underlying channels in the Save dialog box is check
24. cted to a network share Configuration a 20739 Credentials Domain Usermame Password Network Share Path 3 Plug in a USB Ethernet Adapter into a USB port When a live Ethernet cable is connected to the adapter the networking status changes from Adapter Not Ready to Address entry DHCP lt entry gt If the Adapter Not Ready message remains then check the Ethernet cable in a separate device to ensure it is live otherwise obtain a different USB Ethernet Adapter from another vendor 4 Select DHCP under Configuration however if you have a Static IP then select Static IP and complete the open fields Enter Domain NUsername and Password under Credentials Enter Network Share Path and click Login Mapped Drive will change from Not connected to a network share to Connected to lt network share path gt 7 Click OK You will now be able to see your network contents in the Image Review tab or when saving images Note Once networking has been set up the FLoid Cell Imaging a Station retains the information about network configuration and credentials through ON OFF power cycles 23 Using the FLoid Cell Imaging Station General Guidelines Power ON the Instrument To obtain the best results follow these recommendations below Do not operate the instrument in direct sunlight Use the FLoid Cell Imaging Station at room temperature only The F
25. d the Thumbnail view of stored files and folders Continued on next page Image Review Tab continued Image Preview Clicking on a thumbnail view or list view file will display a preview image of the file The file can then be printed or closed g Molecular Probes Reagents Ke Image Acquisition Wa Image Review MSA Presentations fluocells 1 jpg e Print button Brings up the print dialog box for printing the saved file using the selected USB printer e Close Preview button Closes the image preview window 18 User Interface Settings Tab User Interface The Settings Tab on the navigation bar is used for adjusting instrument y Settings Tab settings g Molecular Probes Reagents K3 Image Acquisition Image Review Hot Pixel Correction Time and Date Networking Time 1 14 PM v Software Copy Error Logs Date 10 10 2011 Version No 13746 Printing Language Unit Serial No Default Printer English v 12711 PSMO 003 Canon SELPHY CP800 wv Apply Paper Size 4 x6 100x148mm Postcard v e Hot Pixel Correction Corrects for pixels that no longer respond correctly to incident light e Copy Error Logs Copies error logs to the USB drive e Printing Selects the default printer and paper size e Time and Date Sets the date and time of the device e Language Selects one of seven languages in which the user interface is displayed The available languages are English French Germa
26. e same level of testing requirements 37 General Instrument Safety Moving and Lifting the Instrument Operating the Instrument Cleaning or Decontaminating the Instrument T WARNING PHYSICAL INJURY HAZARD Use this product only as specified in this document Using this instrument in a manner not specified by Life Technologies may result in personal injury or damage to the instrument T WARNING Do not attempt to lift or move the instrument without the assistance of others Depending on the weight of the instrument moving them may require two or more people Things to consider before lifting the instrument Make sure that you have a secure comfortable grip on the computer or the monitor when lifting Make sure that the path from where the object is to where it is being moved is clear of obstructions Do not lift an object and twist your torso at the same time Keep your spine in a good neutral position while lifting with your legs Participants should coordinate lift and move intentions with each other before lifting and carrying Instead of lifting the object from the packing box carefully tilt the box on its side and hold it stationary while someone slides the contents out of the box Ensure that anyone who operates the instrument has Received instructions in both general safety practices for laboratories and specific safety practices for the instrument Read and understood all appli
27. ed see page 30 e Blue Green and Red Lights are fluorescent channels while White Light is for transmitted light images only Continued on next page 25 Using the FLoid Cell Imaging Station continued Focus The FLoid Cell Imaging Station has built in Focus Assist The Focus Assist helps you to find the plane of focus which is represented by a number between 1 and 10 in the Focus Assist Gauge The number displayed in the Focus Assist Gauge should be similar each time the same sample vessel is used T flask Slide a 5 Coverslip 6 well Petri dish e Focus the image using the knobs on the left or right side of the device Focus Knob e The large knob controls coarse focus e The small knob controls fine focus e Focus Assist numbers should be similar each time the same sample vessel is used e FluoCells prepared microscope slides are available as a positive control sample refer to www lifetechnologies com for ordering information Continued on next page 26 Using the FLoid Cell Imaging Station continued Adjust Exposure The Light Slider is used for adjusting image brightness and contrast during image Settings acquisition The slider physically controls the LED intensity camera gain and exposure time e If the black white toggle is depressed on any of the color channels the red color corresponds to saturated pixels This is an indication that the Light slider should be adju
28. ge 33 Changing the Battery continued Changing the 4 Remove the old battery and replace it with a 20 mm coin style 3V lithium battery continued CR2032 battery 20 mm 3V Li coin battery v Note The positive side of the battery must face outward so that the 4 symbol is visible 5 Insert the 4 cover bottom tabs into the microscope base and lift cover top into position Replace the screws 6 Plugin the FLoid Cell Imaging Station and power on 7 Resetthe time and date as described on page 21 34 Appendix A Product Specifications Technical Specifications Physical Characteristics Optics Hardware Software Instrument Type Instrument Dimensions Weight Operating Power Frequency Electrical input Operating Temperature Operating Humidity Output Ports Objective Overall Magnification Contrast Methods Color Channels Illumination Excitation Emission Stage to Condenser Distance Image Acquisition Stage LCD monitor Software Captured Images Supplied USB Drive Benchtop device for imaging cells 15 9 W x 21 1 H x 13 9 D 40 4 cm W x 53 6 cm H x 35 3 cm D 26 lbs 11 8 kg 100 240 VAC 50 60 Hz 5 VDC 4 15 A 4 32 C lt 90 non condensing 4 USB 20X plan fluorite Numerical aperture NA 0 45 Working distance WD 5 9 mm defined as the tip of the objective to the bottom of a 1 2 mm thick slide 460
29. gent information in the Molecular Probes Reagents Tab is organized by application area each represented by an Application Icon Selecting an Application Icon allows you to view the reagent categories corresponding to the selected application area B Molecular Probes Reagents s B Antibody Labeling woe Immunofluorescence Trafficking Protein Labeling Cell Viability Epitope Tags DNA Stains Apoptosis Essentials Ko Image Acquisition 3 Image Review Gj Cytoskeleton Organelle Stains Cell Tracking P Oxidative Stress Cell Proliferation lon Indicators Cell Cycle RNA Biology Reagent Categories Each application area is divided into specific reagent categories The example below shows the reagent categories available for the Immunofluorescence application area g Molecular Probes Reagents Ke Image Acquisition Image Review Immunofluorescence Immunofluorescence Anti Chicken Anti Guinea Pig Anti Mouse Anti Rabbit Anti Sheep Streptavidin Conjugates Anti Goat Anti Human Anti Mouse Isotypes Anti Rat F ab 2 Antibodies Zenon Labeling 14 Continued on next page Molecular Probes Reagents Tab continued Reagent Selecting a reagent category reveals more information about individual reagents Information available in that particular reagent category Reagent Details Protocol toggle is used for switching between reagent details and the product
30. he user interface include e Buttons to operate the instrument e A large viewing pane to visualize sample images e Molecular Probes reagent information with protocols e Thumbnails to confirm image capture e Focus Assist to aid in rapid focusing on the sample e Image processing and automatic image overlay g Molecular Probes Reagents KJ Image Acquisition Image Review Saati 50 Capturing Processing Navigation Tabs The GUI is presented with the following four navigation tabs across the top of the screen e Image Acquisition Tab Used for capturing saving and printing images e Molecular Probes Reagents Tab Provides a reagent selection guide with protocols It contains information over 150 reagents organized by application area Information about each reagent includes a predictive result image and technical specifications e Image Review Tab Displays saved image files and folders This tab can be used to review saved images and organize them into folders e Settings Tab Used for configuring instrument settings such as language preference date and time Image Acquisition Tab Image Acquisition The Image Acquisition Tab allows you to access the main screen of the user Tab interface and it is used to e View samples e Capture images e Overlay color channels e Adjust brightness and contrast of newly captured images e Save images e Print images The Image Acquisition screen can be switched between Capt
31. indicate a warning caution risk of danger Consult the manual to avoid possible personal injury or instrument damage Protective conductor terminal main ground WEEE Waste Electrical and Electronic Equipment symbol indicates that this product should not be disposed of in unsorted municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of WEEE Visit www lifetechnologies com weee for collection and recycling options The CE Mark symbolizes that the product conforms to all applicable European Community provisions for which this marking is required Operation of the instrument is subject to the conditions described in this manual The protection provided by the instrument may be impaired if the instrument is used in a manner not specified by Life Technologies The C Tick Mark indicates conformity with Australian and New Zealand standards for electromagnetic compatibility The ETL Listed Mark is proof of product compliance electrical gas and other safety standards to North American safety standards Authorities Having Jurisdiction AHJ s in 50 states and Canada and retailers accept the ETL Listed Mark as proof of product safety Conforms to UL STD 61010 1 certified to CSA STD C22 2 No 61010 1 This product has been tested to the requirements of CAN CSA C22 2 No 61010 1 second edition including Amendment 1 or a later version of the same standard incorporating th
32. iquids directly on the instrument to avoid electrical shock when the instrument is plugged in e Wipe the instrument exterior using a soft lint free cloth moistened with 70 alcohol Wipe the instrument dry immediately after cleaning e Avoid using a bleach solution because it may leave a residue of bleach crystals on the instrument e Ensure that water or other cleaning solutions do not enter the power switch the power inlet or the USB ports e Never pour or spray any liquids directly on the instrument to avoid electrical shock when the instrument is plugged in 32 Changing the Battery Q IMPORTANT To avoid electrical shock always turn off the FLoid Cell Imaging Station and unplug the power cord before changing the battery n IMPORTANT To avoid risk of fire explosion or burns do not recharge crush heat above 212 F 100 C or incinerate the battery To protect the environment ensure that the battery is fully discharged before disposal Changing the Materials Required battery e 2 5 mm Allen wrench e 20 mm coin style 3V lithium CR2032 battery Procedure 1 Power off and unplug the FLoid Cell Imaging Station 2 Using a 2 5 mm Allen wrench remove all 4 screws from the top edge of the back cover Set the screws aside Remove screws 2 5 mm hex 3 Gently pull the top edge of the cover back and lift cover away from the base of the instrument Set cover aside Continued on next pa
33. ment leave a space of more than 10 cm 4 inches around it and do not place any objects between the instrument and the wall Do not install the instrument on a slant or a place prone to vibrations which induces the risk of instrument malfunction or damage of the instrument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the wall outlet and AC adapter To avoid potential shock hazard make sure that the power cord is properly grounded Be sure to position the equipment such that it is easy to disconnect the instrument Turn off the instrument before unplugging the power cord and or moving the instrument If the instrument is broken or dropped disconnect the power cord and contact a service person Do not disassemble the instrument Use only authorized accessories adaptor power cord and USB drive Do not use metal objects when assembling or dissembling a battery Do not heat the battery or expose it to heat For operating environment see Product Specifications page 35 If the instrument emits smoke disconnect the power cord from the wall outlet and contact a service person Continued on next page 36 Safety Information continued Symbols A CE C CTY LISTED C The symbols used on the FLoid Cell Imaging Station are explained below Used on the instrument to
34. n Italian Japanese Spanish and Simplified Chinese e Networking Allows the setting of network preferences e Software Allows the update of the user interface should updates become available e Unit Serial No Displays the serial number of the FLoid Cell Imaging Station 19 Using the FLoid Cell Imaging Station Getting Started Install the FLoid i Cell Imaging 2 Station 10 11 12 13 14 Open the cardboard shipping container from the top Remove the accessories such as the mouse keyboard and Quick Start Guide and set these off to the side Using the handles lift the inner box containing the FLoid Cell Imaging Station out from within the outer shipping container Set the inner box down on a flat surface such as a table lab bench or floor Unfold the inner box to reveal the FLoid Cell Imaging Station Place the FLoid Cell Imaging Station on a flat level and dry surface such as a lab benchtop Optional Follow the instructions on the Quick Reference Guide see supplied Quick Reference Guide or the section of the User Manual titled Quick Start Guide on page 4 for setting up the device Plug one end of the supplied power cord into the FLoid Cell Imaging Station To the other end attach the appropriate plug adaptor based on the electrical outlet configuration in your country Plug the power cord into the electrical outlet Be sure to use only the power cord supplied with y
35. of fire replace fuses only with fuses of the type and rating specified for the instrument DANGER ELECTRICAL HAZARD Grounding circuit continuity is vital for the safe operation of equipment Never operate equipment with the grounding conductor disconnected DANGER ELECTRICAL HAZARD Use properly configured and approved line cords for the voltage supply in your facility gt gt e DANGER ELECTRICAL HAZARD Plug the system into a properly grounded receptacle with adequate current capacity 41 Biological Hazard Safety A WARNING BIOHAZARD Biological samples such as tissues body fluids and blood of humans and other animals have the potential to transmit infectious diseases Follow all applicable local state provincial and or national regulations Wear appropriate protective eyewear clothing and gloves Read and follow the guidelines in these publications In the U S U S Department of Health and Human Services guidelines published in Biosafety in Microbiological and Biomedical Laboratories stock no 017 040 00547 4 www cdc gov OD ohs biosfty bmb14 bmbl4toc htm Occupational Safety and Health Standards Bloodborne Pathogens 29 CFR 1910 1030 www access gpo gov nara cfr waisidx_01 29cfr1910a_01 html Your company s institution s Biosafety Program protocols for working with handling potentially infectious materials Additional information about biohazard guidelines i
36. onsult the SDS e Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For additional safety guidelines consult the SDS e Check regularly for chemical leaks or spills If a leak or spill occurs follow the manufacturer s cleanup procedures as recommended in the SDS e Comply with all local state provincial or national laws and regulations related to chemical storage handling and disposal 39 Chemical Safety continued Chemical Waste Hazard CAUTION HAZARDOUS WASTE Refer to Safety Data Sheets and local regulations for handling and disposal Chemical Waste To minimize the hazards of chemical waste Safety Guidelines e Read and understand the Safety Data Sheets SDSs provided by the manufacturers of the chemicals in the waste container before you store handle or dispose of chemical waste Provide primary and secondary waste containers A primary waste container holds the immediate waste A secondary container contains spills or leaks from the primary container Both containers must be compatible with the waste material and meet federal state and local requirements for container storage Minimize contact with chemicals Wear appropriate personal protective equipment when handling chemicals for example safety glasses gloves or protective clothing For additional safety guidelines consult the SDS Minimize the inh
37. our instrument Powering the device with an unapproved power cord may damage the device Plug the keyboard into one of the USB ports along the right side of the FLoid Cell Imaging Station see page 9 for the location of the USB ports To engage the wireless mouse plug the USB wireless connector into a USB port on the right side of the FLoid Cell Imaging Station and move the switch on the bottom of the mouse to reveal the optical sensor Optional To enable printing plug one end of the printer cable into the printer and the other end into a USB port on the right side of the FLoid Cell Imaging Station To enable saving of files place the supplied USB storage device into the front facing USB port on the FLoid Cell Imaging Station see page 9 for the location of the USB port When you are ready to use the FLoid Cell Imaging Station turn on the instrument by pressing the Power switch located on the right side of the instrument to the ON position see page 9 for the location of the Power switch Continued on next page 20 Getting Started continued Set Time and Date Select Language 1 Select the Settings Tab to display the Settings Screen Enter the time of day for your location Time and Date Time format is hours minutes and am or pm Time 12 jO Enter the correct date for you location Date format is Month Day Year Date 1 1 Click set time date to save the changes Once the time and da
38. ov te celllmadihigStatolios usa bishop e dte tet em eed 24 SOrbWare DUDOE ades assi m M iac ru donnent mu dore Eden mu doses Ern nada Du dosmea duet Du dose a RE ea E 31 Cleaning theod Cell Imaging Station iias tacathsaecctnesnsaecsecuscatacassacuheasecatnsasesauascmactnsctneataeaansatetomseauasasnact 32 Changingthe Battery e na E E 33 Changing the Baltery enesenn anp ean UTR 33 Appendix A Product SPCCITICAIONS sirsie to anu bunS utra Dcufi bs Deut oe heu fu tu auis 35 Technical SSC ICAO MNS ia E E 30 Append B Sale cae an mene rene en mt Cm reno en er O A tutu eee ne No ee ee rere 36 Se UY TYPO IMAI M 36 Genera In SURITHETIES eel yee aa E E T T A E ae 38 Ge ged gal kore scili M a E A eRe ee nee 39 SUC a aU sai Mica Uu Miu MM uM M M E 41 Biological Hazard Sia CU siis gaket pipi Rite Eyaku t dui i Rat uU xa Rud dp pi Rite p isa Kut quie Robe tust quip a a heated dm ti 42 VOI KS Ca VOI Sate Ey MT PEE EE T I M MITTEN 43 wj H EE 44 Documentation and SUP DOM asana reteset aa trea a O uc im albe dots Gideon eode 45 OP Pail SUDO Ded sone tema bM OPEM EENONNeMIMPOMIMMMENDINE MEME INIM aM 45 Quick Start Guide Installing the FLoid Cell Imaging Station Using the FLoid Cell Imaging Station Remove the FLoid Cell Imaging Station and its accessories from the packaging Place the instrument on a flat dry surface and connect the
39. perform the protocol from start to finish using the selected reagent Sample Specifies whether the reagent is to be used on live or fixed samples An asterisk indicates whether the fluorescence from live samples will be retained after fixation Amount Displays the amount of product supplied based on the product number listed Concentration Lists the concentration of the product as supplied If the product is a dry material then the volume and type of solvent needed for preparing a stock solution is also listed MW Displays the molecular weight or formula weight for chemical reagents Storage Indicates the storage condition for the product Continued on next page Molecular Probes Reagents Tab continued Protocol g Molecular Probes Reagents Ke Image Acquisition Image Review Anti Mouse Reagent Details Anti Mouse 1 Briefly centrifuge secondary antibody 2 Ab 2 Make a 2 ug mL 2 Ab solution in PBS 2 serum 3 Add solution to fixed permeabilized and 1 Ab labeled cells or tissue 4 Incubate 60 min at 22 C 5 Wash sample 2 x 5 min with PBS Materials needed Phosphate Buffered Saline PBS Serum ProLong or SlowFade antifade reagent 6 Add PBS or treat with ProLong or SlowFade antifade reagents ke Ota d 7 Image Sample Protocol screen contains the instructions for using the selected product and includes the following information e Materials Needed List the m
40. s available at www cdc gov In the EU Check your local guidelines and legislation on biohazard and biosafety precaution and the best practices published in the World Health Organisation WHO Laboratory Biosafety Manual third edition www who int csr resources publications biosafety WHO CDS CSR LY O 2004 11 en 42 Workstation Safety Correct ergonomic configuration of your workstation can reduce or prevent effects such as fatigue pain and strain Minimize or eliminate these effects by configuring your workstation to promote neutral or relaxed working positions CAUTION MUSCULOSKELETAL AND REPETITIVE MOTION HAZARD These hazards are caused by potential risk factors that include but are not limited to repetitive motion awkward posture forceful exertion holding static unhealthy positions contact pressure and other workstation environmental factors To minimize musculoskeletal and repetitive motion risks e Use equipment that comfortably supports you in neutral working positions and allows adequate accessibility to the keyboard monitor and mouse e Position the keyboard mouse and monitor to promote relaxed body and head postures 43 SDSs SDSs Obtaining SDSs Chemical manufacturers supply current Safety Data Sheets SDSs with shipments of hazardous chemicals to new customers They also provide SDSs with the first shipment of a hazardous chemical to a customer after an SDS has been updated S
41. sted to a lower percentage g Molecular Probes Reagents K Image Acquisition 3 Image Review Ge gt e Light slider adjusts brightness and contrast of a live image e Light slider combines LED intensity camera gain and exposure time e Light percentages always correspond to a set LED intensity camera gain and exposure time e Black White toggle reveals saturated pixels as a red color Continued on next page 27 Using the FLoid Cell Imaging Station continued Capture e Totemporarily store a sample image click the Capture button This action will create a thumbnail of the image which can then be permanently saved to a USB memory device or printed J Molecular Probes Reagents K Image Acquisition 34 Image Review Lint 80 Capturing Processing e Clicking Capture only temporarily saves images e You can only capture up to four images one of each color channel e To capture additional images save the color channel see page 30 and then delete the thumbnail Continued on next page 28 Using the FLoid Cell Imaging Station continued Image Processing e Clicking on the Processing toggle in the Image Acquisition tab after at least one color channel has been captured allows you to adjust the brightness and contrast of the captured image g Molecular Probes Reagents K Image Acquisition Image Review 100 um o 40 C 10 4 Capturing Processing e
42. tation quality control and commercial services such as reporting the results of purchaser s activities for a fee or other form of consideration For information on obtaining additional rights please contact outlicensing dlifetech com or Out Licensing Life Technologies Corporation 5791 Van Allen Way Carlsbad California 92008 TRADEMARKS The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners 2012 Life Technologies Corporation All rights reserved Contents CMEC dioec MEIST To EROR TT TEE T T 4 POTICO mili ateeseca tuted a asain T T 5 7A O01 0 Ly da oom C100 gt ER T D Froduct Inrermettollsssentscatiwate set ecataaetrsteustacateuatanatansiuretauetantoutiasteauecaenntnadt 6 wae clbnijyce EE 6 Description of the FLoid Cell Imaging StetlOEisxenen uen nter dde tite dice enit reb ssec tlle steric ein 7 ELoid Cellimading station User Ot T ae Bo s retire tetti te ottiene dd tti 10 Mage ACHU NON AD iccessustcisduimaierapectain 11 Molecular Probes Reagents gt open tener meme en ae nee eee de SU ee ae eee 14 fsor fe s duy d NIE ome 17 WSerinteriace SetUnGS MIE oe Pe A A E AAS 19 Using the FLoid Cell Imaging Station cccccsssscccscessssseeeeessenseeeesseeeeeeeesseeteeeeesseneess 20 EUs SAU Sh eT m R 20 Using ithe m
43. te is set there is no need to set it each time the instrument is turned on f 2012 The default language for the FLoid Cell Imaging Station is English To display the user interface in one of the seven available languages follow the English instructions below 1 Select the Settings Tab to display the Settings English Screen French Click the arrow key to display the Language German drop down menu and select the desired Italian language Japanese Click Apply to display the user interface in the selected language Once the language is spanish set the device will default to this selection the next time it is turned on Language simplified Chinese Continued on next page 21 Getting Started continued Hot Pixel Perform Hot Pixel Correction once per month to ensure correct image data This Correction procedure is a subtle correction that is applied to mask the values of pixels that are erroneously reporting a signal Therefore the correction is performed by blocking all light from entering through the objective opening on the stage 1 Select the Settings Tab to display the Settings Screen 2 Click Fix Pixels 3 Follow the onscreen instructions and cover the objective opening on the stage with a flat opaque material such as a lab notebook 4 Click OK to perform the pixel correction procedure g Molecular Probes Reagents K3 Image Acquisition Image Review Hot Pixel Correction Time and
44. uring and Processing screens using the Capturing Processing toggle located at the bottom right of the screen Capturing Screen The Capturing screen contains the controls for capturing images of your sample Image Pane Light Switch OFF A g Molecular Probes Reagents T K Image Acquisition Image Review f RRES pp N alll Light Switch ON Text Box EB Image Thumbnails Capture Button jh k L ji Focus Assist Gauge Light Intensity Slider Capturing Processing Toggle Black White Toggle Continued on next page Image Acquisition Tab continued Capturing Screen continued Image Pane Displays live camera feed or captured images Focus Assist Gauge Tracks the position of the objective lens to help aide in focusing on the sample The number output should roughly be the same each time a particular sample vessel is used e g six well dish 6 1 75 5 Remember this number as it will be useful to find focus faster in the subsequent experiments Black White Toggle Changes the image pane display from color to black and white which can be useful for focusing or removing human eye bias because of color Light Intensity Slider Changes the apparent brightness of a sample being viewed in the image pane The light intensity can be adjusted between 1 and 100 in increments of 10 for Blue Green and Red channels or in increments of 1 for White Light Note The Light Instensity
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