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GS50 Gradient Pump Operator`s Manual

1. RELAY CONTRO READY OUT GND F2 GND LOAD GND Not Used CBL PRESEN GND L lt E e iu v eo a 2 i e e GND Not Used AS40 Relay Control Cable PIN 047946 Co Chromatography Workstation Figure D 5 Example TTL and Relay Connections AS40 D 6 Doc 031612 03 10 03 Part Number 052840 058032 048722 059283 057913 014895 055870 047660 047657 055711 055709 055752 052840 058032 048722 059283 057913 014895 055870 055712 055710 055752 046203 049136 044126 Doc 031612 03 10 03 E Reordering Information Item Primary Pump Head Components Piston Seal Wash Housing Guide Piston Rinse Seal O Ring Spacer with Drain O Ring Piston Seal Inlet Check Valve Assembly Outlet Check Valve Assembly Primary Pump Head Priming Valve Knob Priming Valve Knob O Ring Secondary Pump Head Components Piston Seal Wash Housing Guide Piston Rinse Seal O Ring Spacer with Drain O Ring Piston Seal Secondary Pump Head Waste Valve Knob Waste Valve Knob O Ring Eluent Proportioning Valve Assembly GM 4 Gradient Mixer Glass Reservoir shatterproof plastic coating 1 Liter Quantity nn R es GEEEE R ph 2 E 1 GS50 Gradient Pump Part Number 044127 044128 044129 045987 046594 042772 054578 954745 921019 043598 Item G
2. Tighten liquid connections to the proportioning valves no more than fingertight plus one quarter turn Overtightening or crossthreading the valve fittings may strip the threads in the block CAUTION 8 Align the new valve assembly as shown in Figure 5 5 and mount it to the bulkhead Tighten the screws 9 Push the mechanical chassis drawer back in place Make sure the cables are not pinched and then reinstall the drawer lock screw 10 Attach the liquid line from the inlet of the primary pump head see Figure 5 1 ll Prime the pump see Section B 2 7 Doc 031612 03 10 03 5 13 GS50 Gradient Pump 5 6 Changing Main Power Fuses 1 Turn off the main power switch HIGH VOLTAGE Disconnect the main power cord from its source and also from the rear panel of the GS50 DANGER HAUTE TENSION D branchez le cordon d alimentation principal de sa source et du panneau arri re du GS50 DANGER HOCHSPANNUNG Ziehen Sie das Netzkabel aus der Steckdose und der Netzbuchse auf der R ckseite des GS50 ACHTUNG 2 The fuse holder is part of the main power receptacle on the GS50 rear panel see Figure 5 6 Note the recessed lock located on each side of the fuse holder Using a small screwdriver push each lock toward the center to release it When both locks are released and the fuse holder pops out slightly pull the fuse holder straight out of its compartment 3 Replace the two fuses in the holder with new IEC 127 fast blow
3. J DIONEX GS50 GRADIENT PUMP OPERATOR S MANUAL 2003 Dionex Corporation Now sold under the Thermo Scientific brand Thermo SCIENTIFIC Document No 031612 Revision 03 October 2003 2003 by Dionex Corporation All rights reserved worldwide Printed in the United States of America This publication is protected by federal copyright law No part of this publication may be copied or distributed transmitted transcribed stored in a retrieval system or transmitted into any human or computer language in any form or by any means electronic mechanical magnetic manual or otherwise or disclosed to third parties without the express written permission of Dionex Corporation 1228 Titan Way Sunnyvale California 94088 3603 U S A DISCLAIMER OF WARRANTY AND LIMITED WARRANTY THIS PUBLICATION IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND DIONEX CORPORATION DOES NOT WARRANT GUARANTEE OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATIONS REGARDING THE USE OR THE RESULTS OF THE USE OF THIS PUBLICATION IN TERMS OF CORRECTNESS ACCURACY RELIABILITY CURRENTNESS OR OTHERWISE FURTHER DIONEX CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR CHANGES TRADEMARKS Chromeleon DX LAN PeakNet Self Regenerating Suppressor and SRS are trademarks or registere
4. Pull out the mechanical chassis drawer a few inches d Locate the distribution card on top of the mechanical chassis Check that all electrical cables are seated correctly in their connectors on the card The cables are identified by printed labels on the card If the connections are correct either the distribution card or the vacuum degas assembly may need to be replaced Contact Dionex Technical Support for assistance e Push the mechanical chassis drawer back in place being careful not to pinch the cables Tighten the drawer lock f Turn on the power 4 10 Doc 031612 03 10 03 4 Troubleshooting 4 7 Vacuum Degas Assembly Calibration Fails At the end of the degas calibration the DEGAS READING value is less than 13000 counts and one of the following error messages appears Vacuum degas pump is not present or degas GCLECUDUrY 28 Malfunction ng Vacuum Degas Fails Follow the procedure below to verify that the cable to the vacuum degas assembly is connected to the distribution card l 2 Turn off the GS50 power by pressing the power switch Using a 7 mm open end wrench or your fingers loosen the lock on the mechanical chassis drawer The lock 1s on the lower right side of the chassis between valves 3 and 4 see the label on the inside of the lower door Pull out the mechanical chassis drawer a few inches The distribution card is on the top of the mechanical chassis Locate the vacuum degas assembly co
5. The outlet check valve assembly housing has a 10 32 port Replace the cartridge in the outlet check valve housing making sure the single hole end of the cartridge is visible Liquid flows through the check valve in the large single hole and out the small double holes NOTE The pump will not operate properly unless the cartridge is installed in the housing in the correct orientation 5 2 Doc 031612 03 10 03 5 Service 7 Reinstall the check valves Be sure to install the inlet check valve on the bottom of the primary pump head and the outlet check valve on the top of the head Tighten the check valves fingertight and then use a wrench to tighten an additional one quarter to one half turn Tighten a ittle more only if the valve leaks Overtightening may damage the pump head and check valve housing and crush the check valve seats CAUTION 8 Reconnect the liquid lines Turn on the GS50 main power 9 Prime the pump see Section B 2 7 If you cannot prime the pump and all other possible causes of the problem have been eliminated replace the check valve inlet check valve assembly P N 047660 outlet check valve assembly P N 047657 After replacing the check valve assembly go to the USAGE LOG screen see Section C 2 4 Move the cursor to the VALVES IN USE field and press Enter to reset the field to 0 cycles Doc 031612 03 10 03 5 3 GS50 Gradient Pump 5 2 Re placing a Piston Seal and Piston Rinse Seal A damaged
6. Use the TIME FUNCTION IN screen to e Display GS50 functions that can be controlled via TTL input from another device e Select a TTL signal mode for each function See Appendix D for more information about TTL controlled functions and connections TIME FUNCTION IN MODE INVERTED EDGE PUMP MOTOR OFF ON TTL1 HOLD RESET INIT COND RUN TTL2 Help prompt Figure C 10 Time Function In Screen MODE Selects the signal mode NORMAL EDGE INVERTED EDGE NORMAL PULSE or INVERTED PULSE that corresponds to the signal type of the controlling device The default mode NORMAL EDGE is compatible with the TTL output signals provided by Dionex modules Doc 031612 03 10 03 C 13 GS50 Gradient Pump C 2 Diagnostic Screens C 2 1 C 14 Diagnostic Menu The DIAGNOSTIC MENU lists the diagnostic screens To go to the menu select option 8 from the MENU of SCREENS DIAGNOSTIC MENU 1 POWER UP SCREEN 4 USAGE LOG 2 PRESSURE STATISTICS 5 DIAGNOSTIC TESTS 3 CALIBRATION MENU Help prompt Figure C 11 Diagnostic Menu There are two ways to view a screen option e Press the numeric button on the GS50 front panel keypad that corresponds to the screen number on the menu For example press 3 to select and display the CALIBRATION MENU e Move the cursor to the field containing the screen number and press Enter To display a brief description of each screen press Help Doc 031612 03 10 03 C User Interface C 2
7. Figure 2 9 Method Screen In summary e The GS50 can store up to 100 methods 0 through 99 in memory The actual total which is usually less than 100 depends on the size of each method and the amount of available memory e Each method can include up to 50 time based steps Each step specifies a time an eluent composition a gradient curve number an injection valve position and a flow rate Step 1 always starts at INIT initial condition Step 2 always starts at TIME 0 0 e Methods are retained in memory after the pump is powered down e The pump can continue running under method control while you enter or edit another method e When you save changes to the currently running method or switch to a different method the method clock continues running unaffected Only those parameter changes which affect the method after the current time will be implemented in the current run 2 22 Doc 031612 03 10 03 2 Description 2 8 3 Eluent Delivery Isocratic Eluent Run The simplest use of the GS50 is to deliver an isocratic unchanging mixture of one or more eluents If more than one eluent is selected the pump delivers a proportional mixture of the eluents based on the percentage of each eluent selected The combined percentages of all eluents selected must total 100 or the pump will not run See Section 3 4 1 for an example of an isocratic method Gradient Eluent Run The GS50 can produce step linear concave or con
8. Materials Wetted materials PEEK PTFE Doc 031612 03 10 03 A 3 GS50 Gradient Pump A 4 Doc 031612 03 10 03 B Installation B 1 Facility Requirements e Make sure the GS50 installation site meets the electrical and environmental specifications in Appendix A e Install the GS50 on a sturdy workbench at a height that ensures convenient viewing of the front panel display Lift the GS50 only from the bottom or side surfaces of the module Lifting by the front doors will damage the door hinges Use caution when lifting the module it weighs 19 kg 42 Ib CAUTION Ne soulevez le GS50 que par le fond ou les c t s Son soul vement par la porte du panneau avant endommagera les charni res de la porte Soyez prudent lorsque vous soulevez le GS50 il p se 19 kg MISE EN GARDE Wenn Sie den GS50 anheben oder bewegen mochten greifen Sie bitte unter den Boden oder heben Sie das Gerat an den Seiten an Heben Sie das Gerat nicht an der Vordert r an Dadurch konnen die VORSICHT T rangeln besch digt werden Seien Sie vorsichtig wenn Sie den GS50 anheben Das Gerat wiegt 19 kg e Allowatleast 6 cm 2 5 in of free space behind the GS50 for connections and ventilation e House eluents at least 20 cm 8 in above the GS50 in an EOI Eluent Organizer P N 044125 or in built in eluent containment for example the reservoir container of the LC25 Chromatography Oven Doc 031612 03 10 03 B 1 GS50 Gradient Pump B 2
9. starved for eluent Replace the tubing and fittings and then reprime the pump see Section B 2 7 If priming does not eliminate excessive pressure fluctuations check valves may be dirty or defective Replace the check valves see Section 5 1 Also install an end line filter P N 045987 on each eluent line see Section 3 1 2 impurities in the eluent can cause dirty or defective check valves If priming does not eliminate excessive pressure fluctuations piston seals may be dirty or defective Replace the piston seals see Section 5 2 If priming does not eliminate excessive pressure fluctuations piston may be scratched or broken Replace the piston see Section 5 2 and the piston seal see Section 5 3 Doc 031612 03 10 03 4 Troubleshooting 4 2 Pump Does Not Start Flow rate is set to zero Reset the flow rate see Section 2 4 1 e While being primed pump starts briefly and an alarm sounds 1 The high pressure limit was tripped and the following message is displayed High Pressure Limit Violation Check that the waste valve on the secondary pump head is opened see Figure 2 6 To open the waste valve turn the knob one quarter to one half turn counterclockwise 2 The low pressure limit was tripped and the following message is displayed how Pressure Limit Violation a Verify that the low pressure limit setting 1s several hundred psi below the operating pressure see Section 3 1 5 b Make sur
10. O Ring SI PIN 059283 _ Piston PIN 052840 gt Seal Wash Housing Guide P N 058032 5 Service O Ring P N 055752 H Primary Pump Head P N 055711 O Ring P N 014895 Inlet Check Valve Assembly P N 047660 Figure 5 2 Primary Pump Head Waste Valve Knob 7 PIN 055710 lt gt Piston Seal P N 055870 oO i Spacer with Drain lt PIN 057913 Piston Rinse Seal P N 048722 EN Seal Wash d N eens J PA See O Ring PIN 059283 PIN 052840 WZ A JS Secondary Pump Head P N 055712 PIN 014895 Figure 5 3 Secondary Pump Head Doc 031612 03 10 03 GS50 Gradient Pump Installing the New Piston Rinse Seal l Remove the seal wash guide from the spacer to expose the piston rinse seal and O ring Remove the O ring Remove the old piston rinse seal from the guide as follows a Hold the guide with the flat side facing up b To dislodge the piston rinse seal gently insert the shaft of the piston through the small hole in the center of the guide see photo below c Pull the seal off the end of the piston shaft and remove the piston from the guide Hold the new piston rinse seal P N 048722 with the grooved side facing up Using your fingertip gently press the piston rinse seal into the guide until the edge of the seal is below the surface of the guide The piston rin
11. and pin 2 is Ground Connect the other end of the plug to one of the following a recorder a Dionex UI20 Universal Interface or an A D converter The output signal is equivalent to 1 mV psi 1 e 1000 psi 1 volt out TTL RELAY A strip of eight relay and TTL connectors is located in slot 4 of the GS50 electronics chassis These connectors interface with other devices for relay and TTL control of the pump Refer to Appendix D for more information CPU LAN Card Control Moduleware for the pump resides on the CPU LAN card located in slot 5 of the GS50 electronics chassis A 60 pin ribbon cable links the CPU logic to the front panel display and keypad The logic monitors the internal power supply output The color of the LED at the bottom of slot 5 indicates the status of the power supply output e A green LED indicates normal operation e A red LED indicates a power fault When a power fault occurs the GS50 enters a diagnostic state and inhibits all other controls until the fault is corrected If this occurs turn off the power for a few seconds and then turn it on again Mechanical Chassis The GS50 mechanical chassis is housed n a drawer located behind the lower door of the enclosure Open the mechanical chassis drawer only when components inside the drawer require servicing Before resuming routine operation push in the drawer and tighten the lock in the lower right corner of the chassis Doc 031612 03 10 03 2 11 GS50 G
12. enter a new number to save the method to and press Enter 3 3 2 Running a Method l 2 If the pump motor is off press Off On to turn on the motor Check that the pump is set to LOCAL mode METHOD control Go to the MAIN or DETAIL screen If REMOTE or DIRECT CNTRL is selected move the cursor to the field press Select A or Select V to toggle the value and press Enter or a cursor arrow button In the METHOD field enter the number of the method to run If the method clock is already running the method starts immediately If the clock is in Hold press Hold Run to start the method The elapsed time on the method clock when the method begins determines where that is at which step and parameters the method begins running e If the method clock is at INIT or time zero the method begins running using the INITial condition parameters e Ifthe method clock is greater than zero the method begins running using the parameters specified in the step for that elapsed time If you prefer to start the method at the INITial condition press Reset Doc 031612 03 10 03 3 Operation and Maintenance 3 3 3 Editing a Method Existing methods can be modified by changing adding or deleting steps and parameters Changes can be made while the method clock is stopped or running NOTE After saving changes there is no way to recall the original method If you plan to make experimental changes to a method but also need to retain the ori
13. replace the component Replace as many components as necessary to resume operation at the standard operating backpressure 4 Verify that the pressure transducer offset reading 1s correct a Open the waste valve on the secondary pump head see Section 2 5 by turning the knob one quarter to one half turn counterclockwise b Check the pressure reading if it is above 97 kPa 14 psi recalibrate the pressure transducer offset see Section C 3 5 Doc 031612 03 10 03 4 5 GS50 Gradient Pump e A DSP related error message is displayed Several error messages report problems related to the digital signal processor DSP program DSP communication fails DSP does not acknowledge etc When one of these messages is displayed follow the procedure below 1 Turn off the GS50 by pressing the main power switch 2 Verify that the DSP card is correctly installed in slot 1 of the GS50 electronics chassis see Figure 2 5 Turn on the GS50 power 4 Ifthe DSP error message reappears contact Dionex for assistance One of these parts may need to be replaced the power supply P N 046440 the DSP card or the CPU LAN card Do not remove any of the electronics cards from the pump The components on the cards cannot be serviced by the user If servicing is required it must be performed by qualified personnel and CAUTION appropriate electrostatic discharge ESD handling procedures must be followed Ne retirez aucune des cartes lect
14. the GS50 to operate either with or without reference to time based events The GS50 is usually controlled e Locally from the front panel keypad and display or e Remotely via the Dionex DX LAN interface from a computer running one of these Dionex chromatography software products Chromeleon Release 6 1 or later or PeakNet 6 Release 6 1 or later Limited remote control is available from any device capable of providing compatible TTL signals to the pump The GS50 can function as a stand alone instrument or as part of a chromatography system The chromatography system may include non Dionex instruments provided that they meet the GS50 interface requirements for software TTL or relay control GS50 Options e Vacuum degas assembly The vacuum degas assembly provides online degassing of up to four eluents at times and durations specified by the user Dionex strongly recommends vacuum degassing eluents in order to help prevent bubbles caused by eluent outgassing from forming in the eluent proportioning valves pump heads and detector flow cell The vacuum degas assembly must be installed at the factory Doc 031612 03 10 03 1 1 GS50 Gradient Pump e Standard delay volume conversion kit The GS50 is plumbed at the factory to ensure a delay volume of 400 uL Dionex offers a standard delay volume conversion kit P N 056968 that contains the parts needed to replumb the pump to obtain a delay volume of lt 800 uL Incr
15. then pull the knob straight out of the cavity in the pump head 3 Ifthe O ring is removed with the valve knob in Step 2 pull the O ring off the end of the knob see Figure 5 4 If the O ring is not removed with the valve knob insert a thin object without sharp surfaces into the cavity in the pump head and carefully pull out the O ring Do not scratch the cavity IMPORTANT Scratches in the cavity will cause leaks around the base of the knob while the pump is being primed 4 Slide anew O ring P N 055752 over the end of the valve 5 To reinstall the valve containing the new O ring turn the knob clockwise and then tighten fingertight NOTE It is normal to encounter resistance after several rotations of the knob because the O ring is being pushed into the cavity of the pump head QD SE Valve Knob O an P N 055752 Figure 5 4 Waste Valve or Priming Valve O Ring Replacement Doc 031612 03 10 03 5 11 GS50 Gradient Pump 9 9 5 12 Proportioning Valve Assembly Replacement A defective eluent proportioning valve can cause the following problems leaks nonreproducible eluent compositions which may cause retention time shifts and flow restrictions which may cause high backpressure l Turn off the main power switch to ensure that you do not unintentionally start the GS50 If the eluent reservoirs are pressurized turn off the pressure and allow the reservoirs to vent Follow these steps to discon
16. 1 The door opens to provide access to the electronics chassis see Section 2 2 Screen Contrast Information is displayed on the front panel LCD or screen To adjust the screen contrast use the knurled knob in the recess below the keypad see Figure 2 1 Power Switch The main power switch for the GS50 is on the bulkhead behind the upper door of the enclosure An actuator for the main power switch is on the outside of the front door at the lower left corner see Figure 2 1 The actuator functions only when the door is fully closed When the door is open turn the pump on and off by pressing the main power switch on the bulkhead To prevent damage to the pump circuitry and components always wait at least 15 seconds after turning off the power before turning it on again CAUTION Doc 031612 03 10 03 2 1 GS50 Gradient Pump GS50 Gradient Pump MENU of SCREENS 1 MAIN SCREEN 5 MODULE SETUP 2 DETAIL SCREEN 6 PUMP OPTIONS 3 METHO 7 TIME FUNCTION IN D 4 DEGAS OPTIONS 8 DIAGNOSTIC MENU Help prompt Main Power Tab Switch Actuator for opening for adjusting the door screen contrast Figure 2 1 GS50 Display and Keypad Layout 2 1 1 Control Panel Keypad Use the control panel keypad for direct control of GS50 operation or to create and modify a programmed series of timed events called a method e Press the Menu button to display a list of screen options To view a screen press the numeric button
17. 2 Power Up Screen NOTE The POWER UP screen is displayed briefly when the GS50 power is turned on Use the POWER UP screen to check the revision levels ofthe DSP BIOS main DSP code Moduleware and BIOS installed in the GS50 as well as the ID identification number of the DX LAN if connected An asterisk indicates which DSP code is currently running GS50 GRADIENT PUMP DSP MODULEWARE REV nn nn BIOS nn nn BIOS REV nn nn MAIN nn nn DX LAN ID nnnnnn Help prompt Figure C 12 Power Up Screen Doc 031612 03 10 03 C 15 GS50 Gradient Pump C 2 3 Pressure Statistics Use the PRESSURE STATISTICS screen to view statistical data about the pressure transducer see Section 2 4 2 The status values are updated while the screen is displayed PRESSURE STATISTICS PRESSURE xxxx xx PSI MAX xxxx xx PSI MIN xxxx xx PSI DURATION xxxx xx minutes Help prompt Figure C 13 Pressure Statistics Screen If a method is running while the PRESSURE STATISTICS screen is displayed the following message appears Active Method Lowers MIN MAX Resolution To obtain more accurate readings abort the method select the Direct control mode and go to the PRESSURE STATISTICS screen again PRESSURE The measured pressure from the pressure transducer MAX The maximum pressure value for the duration of the test MIN The minimum pressure value for the duration of the test DURATION The duration of the test Testing starts when the
18. 8 3 enter a curve number in the C column NOTE The pump uses the curve number in a step when moving to that step from the one preceding it Because the INIT and TIME 0 0 steps are not preceded by other steps curve numbers cannot be entered for them If a step field is blank has no entry the last selected value for the field remains in effect The v symbol next to the bottom time entry indicates that it is followed by at least one more step Move the cursor to the entry and press the down arrow to see the additional step s The symbol next to the top time entry indicates that it is preceded by at least one more step Move the cursor to the entry and press the up arrow to see the additional step s The gt symbol at the end of a line on the METHOD screen indicates a lateral extension to the line Move the cursor to the end of the line and press the right arrow to display the METHOD events screen Creating a New Method New methods can be created when the method clock is in either Hold or Run 1 Goto the MAIN or DETAIL screen 2 Check that the pump is set to LOCAL If it is not move the cursor to the field press Select A or Select V to toggle the mode to REMOTE and press Enter or a cursor arrow button 3 Go to the METHOD screen Doc 031612 03 10 03 3 Operation and Maintenance 4 Inthe EDIT field enter either the number of the method to be created if you plan to edit an unused method or the number
19. C 21 Index 2 Power Up C 15 Pressure Offset Calibration C 24 Pressure Statistics C 16 Usage Log C 17 Diagnostic Tests screen C 18 Direct control mode 2 20 3 7 Display and keypad specifications A 1 Display panel C 17 Adjusting brightness C 11 Adjusting contrast 2 1 Distribution card 4 10 4 11 DSP card 4 6 Troubleshooting 4 9 DSP program 1 1 2 14 3 9 Error messages 4 6 DX LAN cable Installation B 6 Specifications B 6 DX LAN interface 1 1 2 19 A 3 Installation B 6 Locked Remote mode 2 21 Rear panel connector 2 18 E Editing a method 3 13 Editing a running method 3 21 Electrical specifications A 1 Electronics chassis 2 9 CPU LAN card 2 11 LC COMM connector 2 10 LC LEAK connector 2 9 PRES 2 11 TTL Relay connections 2 11 D 5 Eluent reservoirs See Reservoirs Eluents Aqueous 3 24 4 2 Compressibility factor C 12 Connections to pump B 7 Degassing 1 1 2 16 Filtering 3 2 4 2 4 15 Flow schematic 2 15 Doc 031612 03 10 03 Flushing lines after eluent change 4 13 Gradient runs 2 23 Installation of reservoirs B 1 Isocratic runs 2 23 Specifying composition 3 9 Step gradient 2 24 End line filter See Filter end line 3 2 4 15 English On screen messages C 1 1 Enter button 2 6 Environmental specifications A 1 EO Eluent Organizer 2 18 B 1 Error messages DSP related 4 6 Encoder index not found 4 7 High pressure limit violation 4 3 4 5 Language options C 11 Low
20. CAL amp STATUS 2 DEGAS CALIBRATION 3 FLOW CALIBRATION 4 PRESSURE OFFSET CALIBRATION 5 PRESSURE RIPPLE CALIBRATION Help prompt Figure C 16 Calibration Menu NOTE You cannot select the DEGAS CALIBRATION screen unless the vacuum degas assembly see Section 2 5 is installed There are two ways to view a screen option e Press the numeric button on the GS50 front panel keypad that corresponds to the screen number on the menu For example press 3 to select and display the FLOW CALIBRATION screen e Move the cursor to the field containing the screen number and press Enter To display a brief description of each screen press Help Doc 031612 03 10 03 C User Interface C 3 2 Leak Sensor Calibration and Status Use the LEAK SENSOR CALIBRATION AND STATUS screen to check the status of leak sensor parameters and to calibrate the sensor s e The PUMP column displays the status of the GS50 leak sensor e The EXTERNAL column displays the status of the leak sensor in the chromatography module If no chromatography module is connected to the GS50 the EXTERNAL fields display NONE LEAK SENSOR CALIBRATION AND STATUS PUMP EXTERNAL MEASURED VALUE 0 25 2 50 CURRENT CONDITION DRY CAL CALIBRATION VALUE NONE 2 51 LOW LEAK THRESHOLD NONE 2 41 Help prompt Figure C 17 Leak Sensor Calibration and Status MEASURED VALUE CURRENT CONDITION CALIBRATION VALUE LOW LEAK THRESHOLD Doc 031612 03 10 03
21. DSP TTL RELAY CPU LAN RLY 1 OUT RLY 2 OUT TTL 1 OUT TTL 2 OUT TTL 1 IN TTL 2 IN TTL 3 IN TTL 4 IN POWER SUPPLY GREEN OK RED FAULT FMZ PU AZOM TI Figure 2 5 GS50 Electronics Chassis LC COMM The LC30 Chromatography Oven s RJ 11 serial cable connects to the LC COMM connector in slot 1 of the GS50 electronics chassis When connected here the LC30 can be remotely controlled by a host computer running Chromeleon or PeakNet 6 software LC AIR The cable from the air solenoid valves on the LC10 LC20 LC25 or LC30 connects to the LC AIR connector in slot 1 of the GS50 electronics chassis When connected here the GS50 can electrically actuate the solenoid valves that control the positions of the injection valve and the optional column switching valve To select the valve positions go to either the MAIN screen see Section C 1 2 or the METHOD screen see Section C 1 4 2 10 Doc 031612 03 10 03 2 3 2 Description PRES The PRES pressure output connector in slot 1 of the GS50 electronics chassis records changes in the pump operating pressure The connector 1s for test purposes only To check the pressure insert the plug P N 923617 provided in the GS50 Ship Kit P N 061222 into the PRES connector Pin 1 ofthe connector is Signal pressure
22. I Me VCC CCA cunis dra octets a de EAE de UAE Ve ee A 1 Display and Keypad arr nearest heehee dua a ar la as A I HydiauliCS nee ted ten dede nione Ro ar ae oe ek qx A 2 Control Modes 242550 ee E ES RN date A 3 Vacuum Degas Assembly Optional nn A 3 B Installation B 1 B 2 Facility Reguitements sedes tte eee tae aoe eee ee ee eee B 1 Installation Instr etion 12d oder boe ovr dario Goh ee ned B 2 B21 Power Connection x2 usen dez ver EA FEEDER dtd B 2 B 2 2 Electronics Chassis Connections ne B 4 i Doc 031612 03 10 03 Contents B 2 3 DX LAN Interface Optional B 6 Bd Was Lines sen vaan entente ted B 7 B29 Bluent Line Connecuions 4 4044244244444 4008 bedek B 7 B 2 6 Piston Seal Wash Connections Optional B 8 B27 Priming the Pump eures der RC Pens B 11 B 3 Automatic SRS Power Control Optional B 15 C User Interface C 1 Operational Screens a euros eom RUE men qo eee is d C 2 Cs Menu Or Screens coms ie e em dU RA b mt ae C 2 Cl Main Screen zu 2 se e ed ee dena Feb e data C 3 Co Deal Screens eh ande xU E E eias C 5 CHE Menol See ine eu d aUa E PR et dt C 6 C L5 Method Events Screens swa dex era ER ara C 8 CLO DELIE Opl ons facade ee C 9 CET Module Setup n2 sataliet e dork whine ede ale PS C 11 C18 Pimp Options rn dase Dias ee a a a a C 12 CER Time Func sars tenten verden sunt C 13 CZ Diasnostie Screens iss ste ie a es C 14 C2 Diasnos
23. Press the numeric button on the GS50 front panel keypad that corresponds to the screen number on the menu For example press 3 to select and display the METHOD screen e Move the cursor to the field containing the screen number and press Enter To display a brief description of each screen press Help Doc 031612 03 10 03 C User Interface C 1 2 Main Screen The MAIN screen is displayed automatically after the GS50 power is turned on Use the MAIN screen to e Select basic operating parameters in Direct control mode or display the status of basic operating parameters programmed on the METHOD screen in Method control mode e Select the operating mode and control mode e Select the method number to run 100 0 Jo A bes E 2000 ps 0 0 B 2 00 mu min 0 0 C 10 27 m n 0 0 D LOCAL METHOD 2 Help prompt Figure C 3 Main Screen A D Displays the percentage of each eluent A B C and D currently in use The pump cannot run unless the eluent percentages total 100 LOAD INJECT Selects the position of the injection valve load or inject COLUMN Selects the active column A or B in the chromatography module Note that Column B is available only when a column switching valve is installed PSI Displays the system backpressure in psi MPa or bar MPa BAR Select the unit of measure on the PUMP OPTIONS screen see Section C 1 8 mL MIN Displays the eluent flow rate through the pump in milliliters per
24. The current measured voltage from the leak sensor The current condition of the leak sensor dry wet or err error The error condition indicates an open or short circuit To calibrate the sensor press a Select button to select CAL and press Enter The current measured value becomes the new dry calibration value The value saved when the leak sensor was last calibrated The minimum voltage reading interpreted as meaning that the leak sensor is dry a reading below this voltage indicates that the sensor is wet C 21 GS50 Gradient Pump C 3 3 Degas Pump Calibration and Status NOTE You cannot select the DEGAS PUMP CALIBRATION screen unless the vacuum degas assembly see Section 2 5 is installed Use the DEGAS PUMP CALIBRATION screen to check the current pressure reading of the vacuum degas assembly and calibrate the degas assembly DEGAS PUMP CALIBRATION AND STATUS DEGAS READING xxxx STATUS OFF CALIBRATION VALUE xxxx CAL RDY Figure C 18 Degas Pump Calibration and Status Screen DEGAS READING Reports the current degas pressure reading CALIBRATION Reports the calibration value recorded when the VALUE vacuum degas assembly was last calibrated STATUS Reports whether the vacuum degas assembly is on or off CAL To calibrate the vacuum degas assembly press a Select button to select CAL and then press Enter C 22 Doc 031612 03 10 03 C 3 4 Flow Calibration C User Interface Use the FLOW CALIBRATIO
25. and use a wrench to tighten the acorn nuts evenly 12 in lb torque Completing the Procedure 1 Reconnect all liquid lines to the pump head 2 Turn on the main power switch 3 Open the eluent valve 4 Prime the pump see Section B 2 7 Doc 031612 03 10 03 5 3 Pu 5 Service mp Piston Replacement Continued leaking of the piston seal after installation of a new seal assuming the pump head is tight indicates a dirty or scratched pump head and or a broken piston l CAUTION MISE EN GARDE Turn off the main power switch to ensure that you do not unintentionally start the GS50 Disconnect the tube fittings to the pump head with the defective piston see Figure 5 1 Remove the two acorn nuts and washers from the pump head see Figure 5 1 Slowly pull the head and allow it to separate from the housing Carefully disengage the head from the piston by pulling the head straight off and away from the studs Lateral motion while disengaging the pump head from the piston may break the piston Un mouvement lat ral pendant la s paration de la t te et du piston peut casser le piston Vermeiden Sie Seitw rtsbewegungen wenn Sie den Kopf vom Kolben l sen Andernfalls kann der Kolben brechen VORSICHT 5 6 Doc 031612 03 NOTE The piston is not removed with the rest of the pump head assembly because it is captured by a magnetic retention system After removing the pump head hold the shaft of th
26. beginning of the eluent B concentration ramp After a total of 13 minutes 3 minutes of isocratic conditions plus 10 minutes to gradually decrease the amount of eluent A in the mixture while increasing the amount of eluent B the eluent composition is 0 eluent A and 100 eluent B Doc 031612 03 10 03 3 Operation and Maintenance 8 Move the cursor to the next line and enter 15 in the TIME field Move the cursor to B and enter 100 9 Move the cursor to the C field and enter 2 to run gradient curve 2 The eluent composition remains unchanged at 100 eluent B for 2 minutes Figure 3 12 illustrates the completed METHOD screen METHOD EDIT 3 SAVE TO 3 LIMITS 0 5000 PSI TIME A B C D C V FLOW 0 00 100 0 L 1 50 gt 3 00 100 0 gt 13 00 100 0 gt gt 15 00 100 0 Help prompt Figure 3 12 Curved Gradient Method Example Complete 10 Move the cursor to the SAVE TO field and press Enter to save the method to memory 3 4 4 Editing a Running Method Example After entering a method you can change add and delete steps and parameters If the method being edited is currently running the changes are executed when you move the cursor to the SAVE TO field and press Enter unless the modified event has already been executed This example describes how to make the following changes to Method 2 the linear gradient example see Section 3 4 2 e Change the eluent composition at TIME 15 00 from 20 eluent A and 80
27. button B 13 Priming valve Opening 2 12 O ring replacement procedure 5 11 Printed circuit boards 2 9 Connectors 2 9 CPU LAN card 2 11 Distribution card 4 10 4 11 DSP card 4 6 4 9 Repair 4 6 Problems See Troubleshooting Programming the pump 3 15 Gradient eluent run 2 23 Proportioning valves 1 1 2 15 Flushing 3 25 Leaks 4 8 Malfunction 4 14 Priming B 12 B 14 Replacement procedure 5 12 Pump head primary Description 2 12 Priming valve 2 12 Pump head secondary Description 2 13 Waste line B 7 Waste valve 2 13 B 7 Pump heads 2 1 Piston seal wash assembly 2 14 3 23 Priming mode 2 3 Pump motor control 2 3 Pump Options screen C 12 Doc 031612 03 10 03 Index R Rear panel 2 18 DX LAN interface B 6 Fuses 2 18 Power receptacle 2 18 Waste lines B 7 Relay connectors 2 11 See also TTL Relay control Remote control of GS50 1 1 2 21 Remote mode C 4 Reproducibility is poor 4 13 Reservoirs 2 17 Cleaning 3 23 4 14 Glass 2 17 Installation 2 17 B 7 Installation in EO 2 18 B 1 Installation in LC25 container B 1 Plastic 2 17 Shutdown 3 25 Reset button 2 4 3 14 Running a method 3 12 S Safety icons 1 4 1 5 3 2 Safety messages 1 4 Salt crystals 3 25 Schematic eluent flow 2 15 Screen contrast 2 1 Seal replacement 5 7 5 8 Select buttons 2 2 2 5 Self Regenerating Suppressor SRS Automatic shutoff B 15 C 12 Power control 4 13 Service proce
28. ee ers 2 23 3 Operation and Maintenance Jl 3 2 3 3 Getting Ready to Rutt tarn EUR TOO E ak edes 3 Sg Deeds TMCS an merecen edo eA e deb Ruta an AVR 3 ILZ Bllter BUCS x nomas 48 2 0 200er ES EMSJ waden 3 2 3 1 3 Pressurize Eluent Reservoirs lllllllllleslnn 3 2 3 14 Stare 14 5554 93h eeaase ooo wad sree Macte east ates 3 3 3 15 Selecting the Pressure Limits 2i 23 2 lt eeeedbsn8 sane 3 4 3 1 6 Calibrating the Pressure Ripple 22222222 3 5 Running Under Direct Control 0 0 0000 cece eee 3 7 3 2 1 Locked Remote Direct Control 04 3 7 222 Local Direct CONO gota a b a ates ed dd a 3 7 Running Under Method Front Panel Control 3 9 3 3 1 Creating a New Method 0 0 cee 3 10 3 3 2 Runninga Method 0 0 ene 3 12 333 Editio a VIethiod a cate exe ip ae ed 3 13 3 34 Deleting a M6IDTOQ e hereto ter EE de ES 3 14 3 3 5 Changing the Running Method 3 14 3 3 6 Controlling the Method Clock 3 14 Doc 031612 03 10 03 Contents 3 4 Example Methods 222 nas TU EVAOE dd PON ME PESE de 3 15 3 4 1 dsocrauc Method Example a uda ante dre de 3 15 3 4 2 Linear Gradient Method Example 3 16 3 4 3 Curved Gradient Method Example 3 19 3 4 4 Editing a Running Method Example 3 2 3 5 Routine MaImtepange a ss due attri de u
29. eluent B to 20 eluent A 60 eluent B and 20 eluent C e Adda step at TIME 17 00 to make the eluent composition 40 eluent B and 60 eluent C Doc 031612 03 10 03 3 21 GS50 Gradient Pump Figure 3 13 illustrates the METHOD screen as it appears when editing is complete METHOD EDIT 2 SAVE TO 2 LIMITS 0 5000 PSI TIME A B D C V FLOW 5 00 100 0 L 2 00 gt 15 00 20 0 60 17 00 20 0 20 00 20 0 Help prompt Figure 3 13 Edited Linear Gradient Method Example This example assumes that Method 2 the example is currently running 1 Go to the METHOD screen and enter 2 in the EDIT field 2 Move the cursor to the TIME 15 00 step of Method 2 Move the cursor to A and enter 20 enter 60 for B and then enter 20 for C 3 Move the cursor to the TIME field and press Insert Enter 17 in the time field Move the cursor to B and enter 40 enter 60 in the C field 4 Move the cursor to the SAVE TO field and press Enter 5 Press Menu and select either the MAIN screen or DETAIL screen Check the status of the method clock on the screen e Ifthe elapsed time is less than 15 minutes the time for the first change made to the method the changes will be incorporated into this run and executed at the programmed time e Ifthe elapsed time is greater than 15 minutes the changes will not be incorporated into this run If it is necessary to put the changes into effect immediately press Reset to set the method clock t
30. information about the PRESSURE RIPPLE CALIBRATION screen see Section C 3 6 3 6 Doc 031612 03 10 03 3 Operation and Maintenance 3 2 Running Under Direct Control When the Direct control operating mode is selected commands are carried out immediately after they are entered and the selected operating parameters remain in effect until commands to change them are issued This section describes the two types of Direct Control Locked Remote and Local 3 2 1 Locked Remote Direct Control When the GS50 is controlled by either Chromeleon or PeakNet 6 software connecting the pump to the timebase automatically selects the Locked Remote operating mode After time based steps for the software are executed on the server the software sends operating commands directly to the pump All operating changes from the GS50 front panel are disabled To return the GS50 to Local mode clear the Connect check box on the GS50 control panel in Chromeleon or PeakNet 6 To return to remote mode select the Connect check box For more information refer to the online Help or the software user s guide 3 2 2 Local Direct Control To select Local Direct control go to the MAIN or DETAIL screen e If DIRECT CNTRL is displayed the GS50 is already in Direct control mode and no further action is necessary e If METHOD is displayed move the cursor to METHOD and press Select A or Select V to toggle to DIRECT CNTRL Press Enter or a cursor arrow button to
31. pressure limit violation 4 3 4 4 Low vacuum alarm 4 12 Motor drive fails 4 7 TTL2 indicates FLOW NO FLOW 4 13 Vacuum degas fails 4 11 Error sounds 2 3 C 11 Ethernet hub B 6 Example methods 3 15 3 16 3 19 F Facilities required B 1 Fields on screen Blank 2 4 Reverse video 2 2 Filter end line Bacterial contamination 3 24 4 2 Dirty or clogged 4 2 Installation 3 2 4 15 Maintenance 3 24 Flow Calibration screen C 23 Flow rate Calibration C 23 Range A 2 Selection 3 9 Doc 031612 03 10 03 Index Stabilization by DSP 3 9 Flow restrictions 3 24 Flow schematic eluents 2 15 Front panel 2 1 2 3 C 11 Button descriptions 2 5 Display screens 2 7 Keypad 2 2 Ribbon cable 2 11 Front panel control 2 21 Functional description Method control 2 21 Fuses 2 18 A 1 Replacement procedure 5 14 G Generating a method 3 10 GM 4 Gradient Mixer 2 14 B 7 GM 5 Gradient Mixer 2 14 Gradient control specifications A 3 Gradient curves 2 23 Concave 2 23 Convex 2 23 2 24 Linear 2 23 Gradient eluent run 2 23 Curved gradient example 3 19 Linear gradient method example 3 16 Gradient mixer 2 14 Installation B 7 Gradient steps 2 23 GS50 Gradient Pump Changing the delay volume 5 16 Decibel level A 1 Delay volume change 5 15 Dimensions A 1 Liquid flow path 2 15 Maintenance 3 23 3 24 Operating humidity A 1 Operating pressure A 2 Operating temperature A 1 Options 1 2
32. screen is opened to terminate the test press Menu To restart the test press Reset this sets the duration to 0 and sets all status values to the current pressure NOTE For a description of the CALIBRATION MENU refer to Section C 3 Doc 031612 03 10 03 C 2 4 Usage Log C User Interface Use the USAGE LOG screen to check for how long certain GS50 components have been in use The status is updated in real time USAGE LOG MODULE ON nnnnnnnn BACKLIGHT nnnnnnnn SEALS IN USE nnnnnnnn VALVESIN USE nnnnnnnn Help prompt Figure C 14 Usage Log Screen MODULE ON BACKLIGHT SEALS IN USE VALVES IN USE RESET Doc 031612 03 10 03 Reports the total time the GS50 has been powered up in its lifetime Reports the total time the display backlight has been on in its lifetime Reports the total number of pump cycles that the piston seals have gone through Reset this field to 0 after servicing or replacing the piston seals Reports the total number of pump cycles that the check valves have been actuated Reset this field to 0 after servicing or replacing the check valves Resets the SEALS IN USE or VALVES IN USE counter to 0 Move the cursor to the corresponding asterisk field and press Enter Reset the counter for the seals and valves to 0 after servicing or replacing these parts GS50 Gradient Pump C 18 C 2 5 Diagnostic Tests Use the DIAGNOSTIC TESTS screen to test the GS50 electronics com
33. the TIME field for the step to be deleted and press Delete twice Note If you accidentally press Delete once immediately press any button except Delete to restore the original time and step parameters Deletes an entire method On the METHOD screen move the cursor to the TIME field of the INIT step and press Delete twice Hold Run Turns the method clock off Hold and on Run This button functions only when the pump is under Method control see Section 2 8 2 When the method clock is in Hold pressing Hold Run starts the clock The clock starts at the initial step of a new method or if resuming an interrupted method at the time the clock was put in Hold When the method clock is in Run pressing Hold Run stops the clock this holds the method and freezes the current conditions Reset gt Changes the method clock time to INIT and causes the initial conditions specified by the method to occur This button functions only when the pump is in Method control see Section 2 8 2 If the method is running it continues running If the method is on hold the method maintains the initial conditions Table 2 1 GS50 Front Panel Buttons Continued Doc 031612 03 10 03 2 Description Button Function C Menu Doc 031612 03 10 03 The Select buttons step through predetermined options in entry fields In a field with predetermined numeric choices pressing Select A increases the value by one unit pressing Select V onc
34. to pump B 5 Leak control cable 2 9 Solenoid valve connections 2 10 B 5 LC20 Chromatography Enclosure Connections to pump B 5 Leak control cable 2 9 Solenoid valve connections 2 10 B 5 LC25 Chromatography Oven Connections to pump B 4 Eluent containment B 1 LC30 Chromatography Oven Communication with pump 2 10 Doc 031612 03 10 03 Connections to pump B 4 Control of pump power B 3 Solenoid valve connections 2 10 LCD contrast 2 1 Leak alarm 4 7 Leak drain Waste line B 7 Leak sensor Calibration procedure C 21 Leak sensor cable LC10 LC20 B 5 Leak Sensor Calibration and Status screen C 21 Leaks 2 9 3 23 4 7 LED Power supply status 2 11 Limit field Detail screen C 5 Linear gradient curve 2 23 3 16 Liquid crystal display LCD Adjusting screen contrast 2 1 Liquid lines 5 2 Leaks 4 7 Local mode 2 19 2 21 Local operating mode 2 21 Locked Remote mode 2 19 2 21 Selecting 2 21 Low delay volume 1 2 5 15 A 2 Gradient mixer 2 14 Low pressure limit 3 4 A 2 Limit is tripped 4 3 4 4 Selecting 3 4 M Maintenance 3 23 Annual 3 24 Daily 3 23 Every 6 months 3 24 Ongoing 3 23 Routine 3 23 Weekly 3 24 Yearly 3 24 Menu button 2 5 Doc 031612 03 10 03 Index Menu of Screens C 2 Degas Options C 9 Detail C 5 Method C 6 Method events C 8 Module Setup C 11 Pump Options C 12 Time Function In C 13 Menu structure C 1 Method clock 2 22 Controlling D 3 Holding pa
35. valves 3 and 4 see the label on the inside of the lower door c Pull out the mechanical chassis drawer a few inches d Locate the distribution card on the top of the mechanical chassis Check that all electrical cables are seated correctly in their connectors on the card The cables are identified by printed labels on the card e Push the mechanical chassis drawer back in place being careful not to pinch the cables Tighten the drawer lock f Turn on the power Doc 031612 03 10 03 4 7 GS50 Gradient Pump 4 4 CAUTION Liquid Leaks Leak Alarm Defective piston seal 1 Replace the piston seal see Section 5 2 2 Check all connections between the eluent reservoirs and the pump heads Tighten the fitting connections just enough to stop the leak Leaking check valves Replace the check valves see Section 5 1 Proportioning valve leaks Tighten loose fittings If there are no loose fittings replace the valve see Section 5 5 Overtightening the fitting connections may strip the threads in the proportioning valve block If this happens the entire proportioning valve assembly must be replaced see Section 5 5 Waste valve knob leaks Replace the O ring P N 055752 See Section 5 4 for instructions Excessive system backpressure If the system backpressure is substantially higher than the normal operating backpressure for the currently configured system including the column tubing may be plugged or overtighten
36. waste line Route this waste line through the lower tubing chase and out the back of the GS50 c Push the 15 cm 6 in piece of clear tubing over the drain tube on the top of the secondary pump head Push the other end of the tubing over the drain tube on the bottom of the primary pump head d Place a 10 mL syringe P N 054578 at the end of the waste line press the syringe against the line to form a seal and draw out all of the air This begins the siphoning action Flush Tubing 117 cm 46 in long Bottle Piston Seal Wash Primary Pump Head Piston Seal Wash A Secondary Pump Head A Tubing 152 cm 60 in long Tubing 15 cm 6 in long Insert black tubing into Waste end of waste line Container Figure B 3 Piston Seal Wash Flow Schematic Doc 031612 03 10 03 B 9 GS50 Gradient Pump e Push approximately 5 cm 2 in of black 0 25 mm 0 01 in ID tubing P N 057057 into the end of the waste line NOTE The black tubing creates a flow restriction Assuming a height difference of about 76 cm 30 in between the flush bottle and the waste container this setup provides a flow of one drop of flush solution every 5 to 30 seconds f Place the end of the waste line in a waste container NOTE For proper drainage the waste line exit should remain above the liquid level in the waste container This completes the piston seal was
37. 1 on or 0 off In Method control program the Relay and TTL fields from the METHOD screen see Section C 1 4 See Appendix D for a description of Relay and TTL control Indicates the gradient curve specified in the current method step See Section 2 8 3 for an explanation of curve types Selects the low pressure and high pressure limits in psi MPa or bar See Section 3 1 5 for guidelines for selecting pressure limits C 5 GS50 Gradient Pump C 1 4 Method Screen Use the METHOD screen to e Enter the number of the method to create or edit e Specify operating parameters for the method e Enter the number the method is saved to METHOD EDIT 5 TIME A INIT 25 0 0 00 100 0 123 45 10 0 345 67 17 2 Help prompt 25 0 25 0 25 0 SAVE TO 6 LIMITS 0 5000 PSI C D C V FLOW L 2 00 gt 22 2 32 2 36 5 5 L 19 6 33 2 30 0 Figure C 5 Method Screen NOTE In some fields 96A B C D C V and FLOW a blank field indicates that the parameter for the previous step remains in effect METHOD EDIT SAVE TO LIMITS TIME The method number 0 through 99 to edit The method number 0 through 99 to save the current method to The low and high pressure limits in psi MPa or bar Select the unit of measure on the PUMP OPTIONS screen see Section C 1 8 See Section 3 1 5 for guidelines for selecting pressure limits The elapsed time for each method step Every method begins with the I
38. 1 serial cable from the rear of the LC30 Chromatography Oven through the GS50 upper chase see Figure B 1 Connect the cable to the LC COMM connector in slot 1 of the GS50 electronics chassis see Figure B 2 2 Route the solenoid valve cable from the rear of the LC30 through the GS50 upper chase Connect the cable to the LC AIR connector in slot 1 see Figure B 2 NOTE Do not connect the LC30 to the LC LEAK connector in slot 1 the oven contains internal leak control electronics LC25 Connections Route the electronics injection valve cable from the rear of the LC25 Chromatography Oven through the GS50 upper chase see Figure B 1 Connect the cable to the LC AIR connector in slot 1 of the GS50 electronics chassis see Figure B 2 NOTE Do not connect the LC25 to the LC LEAK connector in slot 1 the oven contains internal leak control electronics Doc 031612 03 10 03 B Installation Connect the leak sensor cable from the LC10 or LC20 here Connect the serial interface cable from the LC30 here Connect the solenoid valve cable from the LC10 LC20 LC25 or LC30 here PWR SPY SLOT 1 SLOT2 SLOT3 SLOT 4 SLOT5 130W GS50 1825 DSP BLANK BLANK TTL RELAY CPU LAN D RLY 1 L L L OUT 6 E C T RLY 2 L C n OUT Ejoj F TTL 1 F A MI RI 7 OUT R K M O z P TTL2 N F OUT T P2 N z t TTL p IN A D N TTL2 E S
39. 2 Liter Plastikbehalter Bestell Nr 044129 nicht zum Offline Vakkum Entgasen von Eluenten Der Behalter ist dafur nicht ausgelegt VORSICHT Refer to the Pressurizable Reservoir Installation Instructions Document No 034581 for installation details Doc 031612 03 10 03 2 17 GS50 Gradient Pump 2 7 EO1 Eluent Organizer Optional The Dionex E01 Eluent Organizer P N 044125 holds the eluent reservoirs in a liner that contains spills and leaks The EOI can also be used to pressurize the reservoirs Up to two EOI Organizers can be placed on top of the system enclosure The EOI can accommodate either one or two reservoirs depending on whether the reservoirs are made of glass or plastic see the table below for details Number of Type of Reservoir Total Volume Reservoirs 2 Glass or plastic 1 liter 2 liters 2 Plastic 2 liter 4 liters l Glass 2 liter 2 liters Rear Panel The rear panel of the GS50 contains the main power receptacle and fuses It also includes a connector for installation of the DX LAN interface For an illustration of the rear panel see Figure B 1 in Appendix B Doc 031612 03 10 03 2 8 2 Description Functional Description There are three ways to operate the GS50 In Local mode use the front control panel buttons and screens to select operating parameters See Section 2 8 1 for a description of Local mode In Locked Remote mode use Chromeleon or PeakNet 6 to send operating commands fro
40. 45796 Screw M3 x 8 l 045691 Washer l Doc 031612 03 10 03 5 15 GS50 Gradient Pump Conversion procedure 1 Turn off the main power switch to ensure that you do not unintentionally start the GS50 2 Using a Phillips screwdriver remove the screw that secures the mechanical chassis drawer to the enclosure The screw is on the lower left side of the chassis below the secondary pump head see the label on the inside of the lower door Pull out the drawer approximately 15 cm 6 in 3 Remove the tubing assembly installed between the proportioning valve and the primary pump head see Figure 5 7 Disconnect the tubing connected to the inlet check valve housing b Push the tubing through the small opening in the bulkhead near port 2 of the proportioning valve c Disconnect the tubing connected to the proportioning valve outlet and push the tubing through the small opening in the bulkhead near port 3 of the proportioning valve d Remove the tubing assembly from the drawer and set it aside Replace with new Replace with new component clip P N 055860 GM 5 Gradient Mixer P N 054044 of f Pre swage before connecting to valve Replace with new tubing assembly P N 055878 Figure 5 7 Replumbing the GS50 to Change the Delay Volume 5 16 Doc 031612 03 10 03 5 Service 4 Install the new tubing assembly a Locate the tubing assembly as well as the two 1 4 28 fittings
41. B 2 gt 1 gt z z i CAUTION gt CAUTION gt AVERTISSEMENT gt MISE EN GARDE gt WARNUNG gt VORSICHT N Installation Instructions NOTE The GS50 Ship Kit P N 061222 contains several items needed to install the pump Locate the Ship Kit before beginning the installation Power Connection SHOCK HAZARD To avoid electrical shock use a grounded receptacle Do not operate the GS50 or connect it to AC power mains without an earthed ground connection The power supply cord is used as the main disconnect device Make sure the socket outlet is located near the GS50 and is easily accessible Operation at AC input levels outside of the specified operating voltage range may damage the GS50 DANGER D ELECTROCUTION Pour viter toute lectrocution il faut utiliser une prise de courant avec prise de terre Ne l utilisez pas et ne le branchez pas au secteur C A sans utiliser de branchement mis la terre Le cordon d alimentation principal est utilis comme dispositif principal de d branchement Veillez ce que la prise de base soit situ e install e pr s du module et facilement accessible STROMSCHLAGGEFAHR Zur Vermeidung von elektrischen Schlagen ist eine geerdete Steckdose zu verwenden Das Gerat darf nicht ohne Erdung betrieben bzw an Wechselstrom angeschlossen werden Das Netzkabel ist das wichtigste Mittel zur Stromunterbrechung Stellen Sie sicher daf sich die Steck
42. Head Eluent Reservoirs Vacuum Degas Assembly C Chamber IN Secondary A Optional Pump Head VAC Chamber OUT EN ww Tubing Assembly Eluent Proportioning Valve Assembly Figure 2 7 Flow Schematic Doc 031612 03 10 03 2 15 GS50 Gradient Pump 2 5 Vacuum Degas Assembly Optional The vacuum degas assembly provides online degassing of up to four eluents at times and durations specified by the user The degas assembly which must be installed in the GS50 at the factory consists of A four channel degas chamber with degas membranes with internal capacity of 17 mL per channel A dual stage diaphragm vacuum pump A solenoid valve An on board vacuum sensor The electronics required to operate the vacuum pump Tubing fittings and other accessories By default the degas pump turns on for 2 minutes when the GS50 power is turned on Thereafter the degas pump turns on for 30 seconds at 10 minute intervals If necessary go to the MENU of SCREENS select the DEGAS OPTIONS screen see Section C 1 6 and reset the cycle time and duration NOTE The DEGAS OPTIONS screen cannot be selected unless the vacuum degas assembly is installed If necessary go to the PUMP OPTIONS screen see Section C 1 8 and check the DEGAS PUMP OPTION field if the GS50 contains a vacuum degas assemb
43. IN L l TTL 3 DIST 1 pik MOTOR 2 TIL P5 p IN POWER SUPPLY oo N GREEN OK P8 P7 RED FAULT Figure B 2 GS50 Electronics Chassis Located behind pump upper door LC10 or LC20 Connections l Doc 031612 03 10 03 Route the leak sensor cable from the rear of the LC10 Chromatography Organizer or LC20 Chromatography Enclosure through the GS50 upper chase see Figure B 1 Connect the cable to the LC LEAK connector in slot 1 of the GS50 electronics chassis see Figure B 2 Route the solenoid valve cable from the rear of the LC10 or LC20 through the GS50 upper chase Connect the cable to the LC AIR connector in slot 1 see Figure B 2 NOTE Refer to AppendixE for TTL and Relay installation instructions B 5 GS50 Gradient Pump B 2 3 IMPORTANT IMPORTANT B 6 DX LAN Interface Optional The DX LAN interface enables the GS50 to communicate with a host computer running either Chromeleon or PeakNet 6 software The interface also includes the CPU LAN card see Figure B 2 installed in the GS50 at the factory 1 Install the combo IOBASE T Ethernet hub P N 057398 on a workbench or on the wall For installation instructions and site requirements refer to the installation guide shipped with the hub 2 Plug the IOBASE T DX LAN cable P N 960281 into a IOBASE T port on the front panel of the hub The 10BASE T DX LAN cable is a Category 5 unshie
44. LIBRATION screen and run the pressure ripple calibration sequence see Section 3 1 6 Piston seal is damaged If the piston seal is damaged it allows liquid leaks Leaks are usually visible and thus are easily detected If there is leakage in any of the locations described below the piston seal is damaged and should be replaced see Section 5 2 If the piston seal wash assembly is in use Leaks are not visible when the piston seal wash assembly is in use If the piston seal wash assembly is not in use for example because the flush bottle is empty Check for leaks near the drain tube on the bottom of each pump head Doc 031612 03 10 03 4 15 GS50 Gradient Pump 4 16 Doc 031612 03 10 03 5 Service This chapter describes GS50 Gradient Pump service and repair procedures that the user can perform All procedures not included here including electronics related repair procedures must be performed by Dionex personnel For assistance contact Dionex Technical Support In the U S call 1 800 346 6390 Outside the U S call the nearest Dionex office Before replacing any part refer to the troubleshooting information in Section 4 to isolate the cause of the problem IMPORTANT Substituting non Dionex parts may impair GS50 performance thereby voiding the product warranty Refer to the warranty statement in the Dionex Terms and Conditions for more information 5 1 Cleaning and Replacing the Check Valves A dirty check val
45. N screen to recalibrate the pump After running the flow calibration sequence wait at least 15 minutes for the pump to re equilibrate before verifying the flow accuracy FLOW CALIBRATION PRESSURE xxxx PSI SELECT READY PRESS ENTER STABILIZE AT 1 00 mL MIN AT 2000 PSI FOR 50 MINUTES COLLECT WATER INTO A TARED BEAKER FOR 5 MINUTES ENTER THE WEIGHT OF THE WATER x xxxx g WAIT 15 MINUTES BEFORE RE CHECKING Figure C 19 Flow Calibration Screen SELECT PRESSURE WEIGHT OF THE WATER Doc 031612 03 10 03 To start the flow calibration sequence toggle the field from READY to STANDARD CAL and press Enter The pump runs at 1 0 mL min during calibration Reports the current pump pressure in psi MPa or bar Select the pressure unit from the PUMP OPTIONS screen see Section C 1 8 After calibration enter the measured weight of the water pumped into the beaker in this field C 23 GS50 Gradient Pump C 3 5 Pressure Offset Calibration Use the PRESSURE OFFSET CALIBRATION screen to calibrate the pump pressure offset PRESSURE OFFSET CALIBRATION OPEN WASTE VALVE BEFORE STARTING CALIBRATION RDY PRESSURE xxxx PSI OFFSET xxx PSI Help prompt Figure C 20 Pressure Offset Calibration Screen CALIBRATION To begin the pressure offset calibration toggle the field from RDY to CAL and press Enter After confirming that the pump 1s off open the waste valve see Figure 2 6 by turning the knob one quar
46. NIT initial step followed by the TIME 0 00 step Each additional entry under TIME indicates the elapsed time at which user specified conditions eluent percentage curve number flow rate etc occur Doc 031612 03 10 03 A D FLOW Doc 031612 03 10 03 C User Interface The percentage of each eluent occurring at the start of the step The total of all eluents must equal 100 or the pump cannot run The gradient curve type 0 through 9 to use when moving from the previous step to the current step See Section 2 8 3 for an explanation of curve types The position of the injection valve load or inject The flow rate from 0 5 to 5 0 mL min or 0 through the pump The flow rate is adjustable in increments of 0 01 mL min The GS50 is optimized for operation at 1 5 mL min or less The v symbol next to the bottom time entry indicates that it is followed by at least one more step Move the cursor to the entry and press the down arrow to see the additional step s The symbol next to the top time entry indicates that it is preceded by at least one more step Move the cursor to the entry and press the up arrow to see the additional step s The gt symbol at the end of the line indicates a lateral extension to the line Move the cursor to the end of the line and press the right arrow to display the METHOD events screen see Section C 1 5 C 7 GS50 Gradient Pump C 1 5 Method Events Screen NOTE The METH
47. OD events screen can be accessed only from the METHOD screen see Section C 1 4 Use the METHOD events screen to view the column selection and TTL Relay output parameters for each method step METHOD EDIT 5 SAVE TO 6 RUN 10 TTL OUT RLY OUT COL TTL1 TTL2 RLY1 RLY2 TIME A 0 1 0 INIT B 1 0 00 123 45 A v 345 67 Help prompt Figure C 6 Method Events Screen COLUMN Selects the active column A or B in the chromatography module Column B 1s available only when a column switching valve is installed TTL1 Provides TTL and Relay control of other devices TTL2 Select 1 on or 0 off See Appendix D for a RLY1 description of TTL and Relay control RLY2 Doc 031612 03 10 03 C 1 6 Degas Options C User Interface NOTE You cannot select the DEGAS OPTIONS screen from the MENU of SCREENS unless the vacuum degas assembly see Section 2 5 is installed Use the DEGAS OPTIONS screen to set the duration and frequency of the vacuum degas pump DEGAS PUMP DEGAS OPTIONS DEFAULT BY SETTING START UP DURATION 2 MIN 2 MIN CYCLE DURATION 30 30 SEC TIME BETWEEN CYCLES 10 MIN 10 MIN Help prompt Figure C 7 Degas Options Screen DEGAS PUMP START UP DURATION CYCLE DURATION Doc 031612 03 10 03 Specifies how the vacuum degas pump operates e BY SETTING The degas pump runs according to the timing aspects selected on the DEGAS OPTIONS screen e MONITOR The degas pressure reading determines w
48. OTE If you replumb the GS50 for the standard delay volume see Section 5 7 replace the GM 4 with a GM 5 Gradient Mixer P N 054044 Doc 031612 03 10 03 2 Description 2 4 4 Flow Path Figure 2 7 shows the liquid flow path through the pump Mobile phase flows from the reservoir s and through the vacuum degas chambers if the vacuum degas assembly is installed The selected proportions of mobile phase flow out of the proportioning valves through a tubing assembly and to the inlet check valve on the primary pump head The tubing assembly helps to ensure proper delivery mixing and damping of the mobile phase The inlet check valve opens drawing solvent into the primary pump head At the same time the secondary piston pushes forward pushing solvent into the system After completing the intake the primary piston moving at approximately twice the speed of the secondary piston pushes solvent through the outlet check valve and into the secondary pump head The secondary head draws approximately 50 of the volume displaced by the primary piston the remaining 50 is delivered directly into the system The secondary piston delivers the drawn volume into the system during the intake of the primary piston From the secondary head flow is directed through the pressure transducer and then the gradient mixer Pressure Gradient Transducer Mixer To Injection Valve Primary KC UN 7 E Pump
49. P N 052230 and two ferrule fittings P N 052231 included in the package with the tubing From the rear of the bulkhead insert one end of the new tubing assembly through the opening near port 2 and feed the tubing through the opening Install a 1 4 28 fitting and a ferrule fitting on this end of the tubing Connect the tubing to the inlet check valve housing From the rear of the bulkhead insert the other end of the tubing assembly through the opening near port 3 and feed the tubing through the opening Install a 1 4 28 fitting and a ferrule fitting on this end of the tubing Insert the tubing into the drain port on the secondary pump head and tighten to pre swage 1 e compress the ferrule This will improve the connection to the proportioning valve Remove the tubing from the drain port connect it to the proportioning valve outlet and tighten fingertight 5 Disconnect the inlet and outlet lines from the gradient mixer currently installed in the pump Set the mixer aside 6 Remove the hardware component clip washer and screw that secured the gradient mixer to the bulkhead 7 Install the new component clip washer and screw 8 Remove the plug from each end of the new gradient mixer and install the mixer in the clip Connect the inlet and outlet lines to the mixer 9 Push the mechanical chassis drawer back in place making sure the cables are not pinched Reinstall the drawer lock screw 10 Prime the pump see
50. Power switch 2 1 Product warranty 5 1 Index 3 GS50 Gradient Pump Remote control 1 1 Safety messages 3 2 Safety standards 1 4 Ship Kit 2 11 B 2 Spare parts E 1 Weight A 1 B 1 H Help messages 2 5 C 2 C 14 C 20 Language options C 11 High pressure limit 3 4 A 2 Limit is tripped 4 3 4 5 Selecting 3 4 Hold Run button 2 4 3 14 Host computer 2 10 2 19 2 21 Hub Ethernet B 6 Hydraulic specifications A 2 I Important icon 1 4 Initial conditions 2 4 3 11 Injection valve Selecting the position 3 9 Inlet check valve 2 15 Insert button 2 3 Installation B 1 DX LAN interface B 6 Electronics chassis connections B 4 Eluent reservoirs 2 17 B 7 Facilities required B 1 LC LEAK connector 2 9 LC10 connections B 5 LC20 connections B 5 LC25 connections B 4 LC30 connections B 4 Power connection B 2 Pressure limit settings 3 4 Priming B 11 B 14 Rear panel connections 2 18 TTL Relay connections 2 11 D 5 Index 4 Waste lines B 7 Inverted edge D 4 Inverted pulse D 4 Isocratic eluent run 2 23 Method example 3 15 Isopropyl alcohol B 14 J Japanese On screen messages C 11 K Keypad buttons 2 2 Delete 2 4 Enter 2 6 Hold Run 2 4 Insert 2 3 Key press sound 2 3 C 11 Menu 2 5 Numeric buttons 2 6 Reset 2 4 L LC AIR connector B 4 B 5 LC COMM connector 2 10 B 4 LC LEAK connector 2 9 B 4 B 5 LC10 Chromatography Organizer Connections
51. Section B 2 7 NOTE Store the tubing assembly gradient mixer and mounting Doc 031612 03 10 03 hardware removed from the GS50 in a safe place If it is ever necessary to return the pump to the low delay volume these parts must be reinstalled 5 17 GS50 Gradient Pump Doc 031612 03 10 03 A 1 Physical Dimensions Weight Decibel Level A 2 Environmental Operating Temperature Operating Humidity A 3 Electrical Main Power Requirements Fuse Requirements A Specifications 33 5 cm high x 22 5 cm wide x 42 cm deep 13 1 in x 8 9 in x 16 8 in 6 cm 2 5 in clearance required behind the module 19 kg 42 Ib 60 db A WEIGHTING setting 10 to 50 C 50 to 104 F 5 to 95 relative humidity noncondensing 100 to 240 Vac 50 60 Hz 2 5 amps No manual adjustment is required the GS50 power supply is main voltage auto sensing Two 3 15 amp fast blow IEC 127 fuses P N 954745 A 4 Display and Keypad Display Keypad Doc 031612 03 10 03 Liquid crystal with adjustable backlighting 26 button pad for entering commands and numeric values for screen parameters A 1 GS50 Gradient Pump A 5 Hydraulics Eluent Selection Pump Settable Flow Range Proportioning Type Compositional Range Gradient Linearity Flow Accuracy Flow Precision Compositional Accuracy Operating Pressure High Pressure Limit Low Pressure Limit Delay Volume Pressure Ripple F
52. To select the control mode front panel control l 2 Go to either the MAIN or DETAIL screen The control mode field displays either DIRECT CNTRL or METHOD see Figure 2 8 To change the mode move the cursor to th s field press Select A or Select V to toggle to the desired mode and press Enter or a cursor arrow button The table below summarizes the operating and control mode configurations for the GS50 2 20 Operating Control Mode Pump Operation Local Direct Commands are entered from the front panel and executed immediately after being entered Local Method Commands are entered from the front panel and executed by running a method programmed from the front panel or through a TTL input Locked Remote Direct Commands are sent from Chromeleon or PeakNet 6 and executed immediately when received Doc 031612 03 10 03 2 Description 2 8 1 Operating Modes Local Mode When the GS50 power is turned on the pump is in Local mode see Figure 2 8 In Local mode the pump accepts operating commands from two sources e Direct input from the front panel keypad and screens All operating functions are available e TTL inputs from a remote controller such as an integrator or another module The TTL input signals can be used to turn the pump motor on and off or to set the method clock to hold or run Locked Remote Mode In Locked Remote mode the GS50 accepts operating commands from the host computer and Chrome
53. a nun 3 23 3 6 Shuldowmiy 2 34 22 254 dd aarde aX TM AN ERA nee 3 25 4 Troubleshooting 4 1 Pressure Fluctuations Between Pump Heads 4 1 4 2 Pump Does Not Start 245 semens 1 ie 4 3 4 3 Pump OPS esse uda sd d eddy NA Nee doeet sedes esed t 4 4 4 4 Liguid Leaks Leak Alan au 35d ee 4 8 4 5 Noisy Pummp MOtOE s cie ctr tadesdetedesas tar aas 4 9 4 6 Vacuum Degas Assembly Does Not Run 0005 4 9 4 7 Vacuum Degas Assembly Calibration Fails 4 11 4 8 Vacuum Degas Assembly Low Vacuum 22222222 4 12 4 9 Relays or TTLs Inoperative 0 0 0 nn 4 12 4 10 TTL Output Inoperative nnn 4 13 4 11 Poor Chromatographic Reproducibility 4 13 AAD Noisy BaselinGsen swennen lerende er do UE ERBE Ih 4 15 Doc 031612 03 10 03 iii GS50 Gradient Pump 5 Service 5 1 Cleaning and Replacing the Check Valves 5 1 3 2 Replacing a Piston Seal and Piston Rinse Seal 5 4 5 3 Pump Piston Replacement llle 5 9 5 4 Waste Valve or Priming Valve O Ring Replacement 5 11 5 5 Proportioning Valve Assembly Replacement 5 12 5 6 Changing Main Power Fuses ee 5 14 5 7 Changing the GS50 Delay Volume Optional 5 15 A Specifications A l A 2 A 3 A 4 A 5 A 6 A 7 PHYSICA ens s aam ha nete ee as A 1 Environmental 555505513 ar arte era EEA A
54. ables may be installed incorrectly Verify that ground wires are connected to ground pins and signal wires are connected to signal pins For complete installation instructions see Section D 3 2 The TTL input mode selected on the TIME FUNCTION IN screen see Section C 1 9 must match the signal type output by the device connected to the GS50 Check the user s manual for the device to verify that the correct signal type is selected 3 A programming error occurred in the device sending the signal Refer to the user s manual for the device for troubleshooting assistance e TTT Relay output related error l The cables may be installed incorrectly Verify that ground wires are connected to ground pins and signal wires are connected to signal pins For complete installation instructions see Section D 3 Doc 031612 03 10 03 4 Troubleshooting 2 A programming error occurred in the device sending the signal Refer to the user s manual for the device for troubleshooting assistance 3 The device being triggered may require a TTL input not a relay Connect the device to a GS50 TTL output see Appendix D 4 10 TTL2 Output Inoperative When attempting to set TTL2 the following message appears TIL TS set to indicate FLOW NO FLOW The TTL2 OUTPUT USAGE field on the PUMP OPTIONS screen is currently set to signal when pump flow stops 0 FLOW This setting can be used to control the power to a Self Regenerating S
55. activate the selection Doc 031612 03 10 03 3 7 GS50 Gradient Pump Local Direct Control Example Specify an isocratic mixture of 60 eluent A 25 eluent B and 15 eluent C to be pumped at 1 0 mL min see Figure 3 3 Eluent A Eluent Eluent B 15 20 25 Time min Figure 3 3 Isocratic Run Profile l Goto the MAIN or DETAIL screen Figure 3 4 illustrates the MAIN screen as it appears when the example is set up 60 0 A o 25 0 B COLUNN A gt 00 d 15 0 C OO mL m n 0 0 D LOCAL DIRECT CNTRL Help prompt Figure 3 4 Main Screen Running Under Local Direct Control 2 Check that the operating fields are set t0 LOCAL and DIRECT CNTRL If REMOTE or METHOD is selected move the cursor to the appropriate field press Select A or Select V to toggle the value and press Enter or a cursor arrow button 3 8 Doc 031612 03 10 03 3 Operation and Maintenance 3 Movetothe A field and enter 60 Move to the B field and enter 25 Move to the C field and press Enter do not enter a value When you press Enter the Moduleware automatically calculates the percentage required to make the total of all eluents equal 100 In this example eluents A and B already total 85 60 25 85 so the Moduleware will specify 15 of eluent C 100 85 15 4 Move to the mL MIN field and enter 1 5 Ifthe pump is off press Off On to turn on the motor and begin the isocratic delivery NOTE After starting
56. alve on the primary pump head 1s closed see Figure B 4 4 Open the waste valve on the secondary pump head see Figure B 4 by turning the knob one quarter to one half turn counterclockwise Opening the valve directs the eluent flow path to waste and eliminates backpressure 5 Press Prime on the GS50 front panel The pump begins pumping at approximately 2 5 mL min NOTE The vacuum degas assembly contains 17 mL of eluent If the degas assembly is installed prime the GS50 for at least 7 minutes more than usual this ensures that all air and previous eluent are purged from each channel A B C and D of the degas assembly 6 Continue priming the GS50 until all air and previous eluents are purged and no air bubbles can be seen exiting the waste line If the eluent manifold has not been primed allow several extra minutes for the eluent to pass through the pump 7 Press Prime again to return to the flow rate selected for the analysis 8 Close the waste valve Do not overtighten The pump is now ready for operation Doc 031612 03 10 03 B 13 GS50 Gradient Pump CAUTION B 14 Priming with Isopropyl Alcohol NOTE Prime the pump heads with isopropyl alcohol only if the two standard priming procedures see previous sections are unsuccessful Connect a 10 mL syringe P N 054578 filled with isopropyl alcohol IPA to the luer port in the primary pump head see Figure B 4 Open the waste valve on the secondary pump head
57. aste line is installed at the user site when the piston seal wash assembly is connected see Section B 2 6 Place the free ends of the waste lines into one waste container To maintain a positive siphon position the waste container below the level of the GS50 NOTE For proper drainage the leak drain line and seal wash line should remain above the liquid level in the waste container B 2 5 Eluent Line Connections Eluent Inlet Line Connections Eluent lines A through D are plumbed to the GS50 at the factory To complete the installation attach the free end of each line to the corresponding eluent reservoir Eluent Outlet Line Connections Connect the tubing exiting the GM 4 Gradient Mixer to the injection valve see Figure 5 1 NOTE Although the GS50 does not require pressurized reservoirs degassed eluents and pressurized reservoirs are important when using eluents that are manually degassed or eluents that are sensitive to carbonate NaOH contamination For a list of Doc 031612 03 10 03 B 7 GS50 Gradient Pump CAUTION MISE EN GARDE VORSICHT B 2 6 pressurizable reservoirs available from Dionex see Section 2 6 Do not use the 2 liter plastic reservoir P N 044129 for offline vacuum degassing of eluents The reservoir was not designed for this purpose N utilisez pas le r servoir en plastique de 2 litres N P 044129 pour le degazage a vide hors ligne d luants Le r servoir n a pas t concu a cette
58. certain information They alert the user to an unexpected result of an action suggest how to optimize instrument performance etc Doc 031612 03 10 03 1 5 GS50 Gradient Pump 1 3 Safety Labels The TUV GS C US Mark safety label and the CE Mark label on the GS50 indicate that the GS50 1s in compliance with the following standards EN 61010 1 1993 safety CAN CSA C22 2 No 1010 1 92 safety UL 3101 1 10 93 safety EN 50082 1 1992 susceptibility and EN 55011 1991 emissions These symbols appear on the GS50 or on GS50 labels dd Alternating current ds Protective conductor terminal Power supply is on C Power supply is off 1 6 Doc 031612 03 10 03 2 1 2 Description The GS50 Gradient Pump enclosure consists of two units e The control panel is located on the door of the upper unit The electronics chassis which contains the power supply and several electronics cards printed circuit boards is inside the upper unit For a description of the control panel see Section 2 1 For a description of the electronics see Section 2 2 e The lower unit houses mechanical components the eluent proportioning valves pump heads pressure transducer etc For a description of the mechanical components see Section 2 3 Front Control Panel The control panel on the upper door of the GS50 enclosure contains a liquid crystal display LCD a membrane keypad and the actuator for the main power switch see Figure 2
59. d then repeat Steps 2 and 4 Remove the 10 32 fitting plug s Installing the New Piston Seal l Open the priming valve knob primary pump head or waste valve knob secondary pump head by turning the knob one quarter to one half turn counterclockwise Push the piston through the spacer and then through the new seal Insert the piston and seal into the pump head until the seal makes contact with the bottom of the counterbore Remove the piston NOTE If necessary lubricate the seal with a small amount of isopropyl alcohol to facilitate insertion To seat the seal push down on the spacer until it is flush with the head A clicking sound indicates that the seal is correctly seated 4 Close the priming valve knob or waste valve knob Doc 031612 03 10 03 5 7 GS50 Gradient Pump Reinstalling the Head and Piston Dionex recommends reinstalling the head and piston as a single assembly so that the piston centers itself onto the magnetic follower 1 Hold the assembled spacer and stud with the drain tubes aligned vertically and press the spacer into the head until it is flush with the indented surface of the head 2 Insert the piston so that 1 4 inch of the shaft is exposed This ensures that the magnet in the follower picks up the piston The follower is the rod that holds the piston in place as it moves in and out of the pump head assembly 3 Reinstall the head and piston Place the washers and acorn nuts on the studs
60. d trademarks of Dionex Corporation Tefzel is a registered trademark of E I duPont de Nemours amp Company PRINTING HISTORY Revision 01 April 2000 Revision 02 September 2001 Revision 03 October 2003 Contents 1 Introduction 1 1 ONEVIEW auena a bos aaa aaa aa ee Wang daa ee 1 1 2 About his Manual asus nase ee ch ie 1 3 L2 Type S aren wet areas E ERE nit 1 4 1 2 2 Safety Messages and Notes cece eens 1 4 1 3 Safely Labels sn x re eiu drin rn 1 6 2 Description 2 1 Front Control Panel o ouo ai eda eon ao ow a ere erat Aleta tant ate 2 1 2 1 COnttol Panel Keypad cece semens oye hed eee 2 2 2 1 2 Display Screens 223445544 445234 aa RES RS 2 7 22 Electronics CHASSIS vire hrs cd o eden ed ie Odette erde amor 2 9 2 3 Mechanical CHASSIS apron esta E Baar aan 2 11 2 4 Inferior C OMPONENIS sarrus waa ae ra Sew een 2 12 241 Pomp Heads uwer v tex dd aen u ed 2 12 2 4 2 Pressute Transducer 4 2 39 ERE ERES 2 14 243 Gradient MIXER coe duets cee teh sea as 2 14 2 BONA zes ernennt bate do ne tek 2 15 25 Vacuum Degas Assembly Optional 0 0 00 eee 2 16 2 6 Eluent Reservoir ia 2 17 2 7 Rear Panel guts ences nat Bun ee 2 18 Doc 031612 03 10 03 i GS50 Gradient Pump 2 8 Putictonmal DescriDLIOB anaes e ss oda NERENN DRE 2 19 Zelk Operating MOS uec Sor case av a eS SEEN ee 2 21 2 8 2 Method Front Panel Control ullllllsn 2 21 2 83 Bent Delvery za tieke eter eam ee
61. d via the port in the front of the valve see Figure B 4 NOTE The priming valve must be open when the pump is being primed with a syringe or with isopropyl alcohol For detailed priming instructions see Section B 2 7 Doc 031612 03 10 03 2 Description Outlet Check Valve Gradient Mixer Primary Pump Head Secondary Pump Head Acorn Nut Acorn Nut SY li 3 ns gt Lj Proportioning E i PA Waste Valve Priming 7 pee gm 4 es 4 Pressure Transducer Sensor Waste Line Figure 2 6 GS50 Mechanical Components Secondary Pump Head The secondary pump head delivers eluent to the remainder of the chromatography system the injection valve column and detector The waste valve is located on the front of the secondary pump head see Figure 2 6 To open the waste valve turn the knob one quarter to one half turn counterclockwise When the waste valve is in the open position all output is directed to waste NOTE The waste valve must be open when the pump is being primed For detailed priming instructions see Section B 2 7 Doc 031612 03 10 03 2 13 GS50 Gradient Pump 2 4 2 2 4 3 2 14 Piston Seal Wash The GS50 includes a piston seal wash assembly that can be set up to continuously rinse the back of the piston seals Rinsing the piston seals removes salt crystals that can abrade t
62. dose nahe am Gerat befindet und leicht zuganglich ist Doc 031612 03 10 03 B Installation The GS50 power supply is auto sensing thus no adjustment is required to select the line voltage There are two ways to control power to the GS50 On off control from the GS50 To implement Connect a modular power cord IEC 320 C13 from the GS50 main power receptacle see Figure B 1 to a grounded single phase power source Use the GS50 power switch actuator to turn the pump on and off Control from the LC30 Chromatography Oven To implement Locate one of the IEC jumper cables P N 960748 provided in the LC30 Ship Kit Connect the jumper cable from the GS50 main power receptacle see Figure B 1 to an IEC auxiliary receptacle on the LC30 rear panel Leave the GS50 power switch on continuously and use the LC30 main power switch to turn the pump on and off DX LAN Connector Upper Chase TTL amp Relay Lines Lower Chase Eluent and Waste Lines SAFETY WARNING SERIAL NUMBER LABEL Fuse Holder Main Power Receptacle Figure B 1 GS50 Rear Panel Connections Doc 031612 03 10 03 B 3 GS50 Gradient Pump B 2 2 Electronics Chassis Connections Connections to the GS50 electronics chassis vary depending on which Dionex chromatography module is being connected to the pump For instructions refer to the appropriate section below LC30 Connections 1 Route the RJ 1
63. dures 5 1 Check valve cleaning 5 1 5 3 Check valve replacement 5 1 5 3 Fuse replacement 5 14 Piston replacement 5 9 Piston seal replacement 5 4 Priming valve O ring replacement 5 11 Proportioning valve replacement 5 12 Index 7 GS50 Gradient Pump Waste valve O ring replacement 5 11 Ship Kit B 2 Shutdown 3 25 Signal modes for TTL input D 3 Six month maintenance 3 24 Software See Chromeleon software 1 1 See PeakNet 6 software 1 1 Software control of GS50 2 21 Solenoid valve connections 2 10 B 5 Spare parts E 1 Specifications Display and keypad A 1 Electrical A 1 Environmental A 1 Gradient control A 3 Hydraulic A 2 Physical A 1 Vacuum degas assembly A 3 SRS Automatic shutoff B 15 C 12 Power control 4 13 Standard delay volume 1 2 5 15 A 2 Conversion procedure 5 15 5 16 Gradient mixer 2 14 Step gradient change 2 24 T Technical Support 4 1 5 1 Tests Electronics components C 18 Operating pressure 2 11 Pressure transducer C 16 Time Setting the method clock 3 14 Time Function In screen C 13 D 3 Troubleshooting 4 1 Chromatographic reproducibility 4 13 Degas assembly calibration fails 4 11 Degas assembly low vacuum 4 12 Leak alarm 4 7 4 8 Liquid leaks 4 7 4 8 Index 8 Pressure fluctuations 4 1 Pump does not start 4 3 Pump is out of prime 4 1 4 2 Pump motor is noisy 4 9 Pump stops unexpectedly 4 4 Relay controls are inoperative 4 12 TTL2 out
64. e decreases the value by one unit and holding down a Select button increases or decreases the value To confirm the selected value press Enter or a cursor arrow button When the GS50 is running under Method control see Section 3 3 pressing a Select button when the cursor is in the elapsed time field steps you through the programmed steps in the method that is currently running Pressing an arrow button moves the cursor in the direction of the arrow to the next entry field if one exists At the end of a line the left arrow wraps the cursor around to the next entry field on the line above the right arrow wraps the cursor to the next entry field on the line below The up and down arrows do not wrap around Pressing an arrow button immediately after entering or selecting a new value in an entry field saves and or executes the new value just as pressing Enter does except when the cursor is in the following locations DIAGNOSTIC TEST screen fields SAVE TO and RUN fields on the METHOD screen CALIBRATION screen fields and Menu fields Displays context sensitive help information if available When an operational screen is displayed pressing Menu displays the MENU of SCREENS When a diagnostic screen is displayed pressing Menu displays the DIAGNOSTIC MENU When a calibration screen is displayed pressing Menu displays the CALIBRATION MENU Note The Menu button is disabled when a pressure ripple calibration sequence is runni
65. e current limit is being activated even at low flow rates and or low pressures contact Dionex Technical Support for assistance 4 6 Vacuum Degas Assembly Does Not Run Vacuum degas assembly is not installed Go to the PUMP OPTIONS screen see Section C 1 8 and verify that the DEGAS PUMP OPTION field is set to YES If the field is set to NO the vacuum degas assembly is not installed DEGAS OPTIONS screen settings incorrect Go to the DEGAS OPTIONS screen see Section C 1 6 If the DEGAS PUMP field is set to ALWAYS OFF select BY SETTING and then enter the cycle duration and frequency times Electrical cables improperly installed Follow the steps below to manually test the vacuum degas assembly 1 Goto the DEGAS PUMP CALIBRATION AND STATUS screen see Section C 3 3 Press a Select button to toggle the RDY field to CAL and press Enter Doc 031612 03 10 03 4 9 GS50 Gradient Pump 2 The pump should turn on and run for the cycle duration time specified on the DEGAS OPTIONS screen see Section C 1 6 If it does not verify that the cables connected to the pump in the GS50 electronics chassis see Figure 2 5 and mechanical chassis are connected correctly Turn off the GS50 power by pressing the power switch b Using a 7 mm open end wrench or your fingers loosen the lock on the mechanical chassis drawer The lock is on the lower right side of the chassis between valves 3 and 4 see the label on the inside of the lower door
66. e cursor to the line below TIME 0 and enter 5 to store a step at TIME 5 00 minutes Next move the cursor to A and press Enter to enter 10096 and define a step with the same eluent composition as the previous step Although there is no change in eluent parameters 100 of A must be entered at 5 00 minutes to establish 5 00 as the gradient start point This marks the end of the 1socratic section of the run and the beginning of the eluent B concentration ramp From this point on the concentration of eluent A decreases from 100 as the concentration of eluent B increases from 0 GS50 Gradient Pump 5 Move the cursor to the next line Enter 15 in the TIME field Move the cursor to the A field and enter 20 enter 80 in the B field After a total of 15 minutes 5 minutes of isocratic conditions plus 10 minutes to gradually decrease the amount of eluent A in the mixture while increasing the amount of eluent B the eluent composition is 20 eluent A and 80 eluent B Figure 3 8 illustrates the METHOD screen as it appears after Step 5 METHOD EDIT 2 SAVE TO 2 LIMITS 0 5000 PSI TIME A B C D C V FLOW INIT 100 0 0 00 100 0 5 00 100 0 15 00 20 0 80 0 Help prompt Figure 3 8 Linear Gradient Method Example After Step 5 6 Move the cursor to the next line Enter 20 in the TIME field Move the cursor to the A field and enter 20 enter 80 in the B field The eluent composition remains unchanged at 20 eluent A and 80 elu
67. e piston near the base tilt the piston slightly and pull the piston away from the pump Replace the piston P N 052840 and piston seal P N 055870 10 03 5 9 GS50 Gradient Pump 7 Dionex recommends reinstalling the head and piston as a single assembly so that the piston centers itself a Check that the piston rinse seal and O ring are seated in the seal wash housing guide Press the seal wash housing guide into the head until it is flush with the indented surface of the head b Insert the piston into the head so that one quarter inch of the shaft is exposed This ensures that the magnet in the follower picks up the piston The follower is the rod that holds the piston in place as it moves in and out of the pump head assembly c Reinstall the head and piston Place the washers and acorn nuts on the studs and use a wrench to tighten the acorn nuts evenly 12 in lb torque 8 Reconnect the tube fittings to the pump head 9 Turn on the main power switch 10 Prime the pump see Section B 2 7 5 10 Doc 031612 03 10 03 5 Service 5 4 Waste Valve or Priming Valve O Ring Replacement A damaged O ring causes leakage around the base of the waste valve or priming valve knob 1 Turn off the main power switch to ensure that you do not unintentionally start the GS50 2 To remove the waste valve or priming valve from the pump head see Figure 5 1 turn the appropriate knob counterclockwise until it is loose and
68. e tab located to the right of the main power actuator see Figure 2 1 Do not remove any of the electronics cards from the GS50 The components on the cards cannot be serviced by the user If servicing is required it must be performed by qualified personnel and CAUTION appropriate electrostatic discharge ESD handling procedures must be followed Ne retirez aucune des cartes lectroniques de la pompe Aucun des composants sur les cartes ne peut tre r par par l utilisateur Toute r paration doit tre effectu e par un personnel qualifi utilisant des MISE EN GARDE procedures correctes de d charge lectrostatique Halten Sie sich von der Elektronik des GS50 fern Die Elektronik kann nicht vom Anwender gewartet werden Falls ein Service erforderlich ist ist dieser von qualifiziertem Personal durchzuf hren Dabei VORSICHT m ssen die geeigneten Verfahren zur elektrostatischen Entladung ESD eingehalten werden LC LEAK The leak control cable from the LC10 Chromatography Organizer or the LC20 Chromatography Enclosure connects to the LC LEAK connector in slot 1 of the GS50 electronics chassis If a leak occurs in the LC10 or LC20 it is reported to the pump NOTE Do not connect the LC25 Chromatography Oven or the LC30 Chromatography Oven to the LC LEAK connector These ovens contain internal leak control electronics Doc 031612 03 10 03 2 9 GS50 Gradient Pump PWR SPY SLOT 1 SLOT 4 SLOT5 130W GS50 1525
69. e there are no liquid leaks in the flow system c Make sure both the priming valve and waste valve are closed see Figure 2 6 To close a valve turn the knob clockwise Doc 031612 03 10 03 4 3 GS50 Gradient Pump 4 3 Pump Stops e Method or other remote input instructed the pump to stop If no error message is displayed the pump was probably instructed to stop by the method computer or other remote signal source If you do not want the pump to stop take the appropriate action edit the method etc e Low pressure limit was tripped the following message is displayed Low Pressure Limit Violation 1 Verify that the low pressure limit setting is several hundred psi below the operating pressure see Section 3 1 5 2 Make sure there are no liquid leaks in the flow system 3 Verify that eluent is present in the selected channel If the eluent reservoir is empty refill it or select a channel which does have eluent Prime the pump before resuming operation see Section B 2 7 4 Make sure both the priming valve and the waste valve are closed see Figure 2 6 To close a valve turn the knob clockwise 5 Place the pump in LOCAL mode DIRECT CONTROL see Section 2 8 Press Off On to start the pump Verify that the pistons are moving and that pump operation is audible If there is no sound from the pump check the LED on the CPU LAN card in the GS50 electronics chassis see Figure 2 5 A green LED indicates normal operat
70. easing the delay volume will improve mixing noise at flow rates of 1 0 mL min and above The pump must be replumbed at the installation site for instructions see Section 5 7 The following table lists the GS50 product versions available from Dionex System GS50 with Vacuum GS50 without Vacuum Degas Assembly Degas Assembly DX 600 P N 059481 P N 059479 BioLC P N 059493 P N 059491 NOTE The GS50 Gradient Pump is designed to perform ion chromatography and BioLC applications only and should not be used for any other purpose If there is a question regarding appropriate usage contact Dionex Doc 031612 03 10 03 1 Introduction 1 2 About This Manual Chapter 1 Introduction Chapter 2 Description Chapter 3 Operation and Maintenance Chapter 4 Troubleshooting Chapter 5 Service Appendix A Specifications Appendix B Installation Appendix C User Interface Appendix D Relay and TTL Control Appendix E Reordering Information Doc 031612 03 10 03 Presents a brief overview of the GS50 Gradient Pump Explains the meaning of safety messages and icons in the manual and safety labels on the pump Describes physical aspects of the GS50 including the front panel controls rear panel connections electronics and mechanical components Describes the pump operating modes and control modes Describes key operating features and how to create edit and run methods from the GS50 front panel Lists routine preve
71. ed Follow the procedure below to isolate segments of the flow path and determine the source s of the high backpressure 1 Remove the pump outlet tubing at the injection valve 2 Press Off On to turn on the pump Set the flow rate to 1 0 mL min and record the backpressure 3 One at a time reconnect each component tubing fittings etc of the flow path If reconnecting a component causes a sharp increase in backpressure replace the component Replace as many components as necessary to resume operation at the standard operating backpressure Doc 031612 03 10 03 4 Troubleshooting 4 5 Noisy Pump Motor Excessive pressure or worn motor As the pump motor ages it typically becomes noisier and a slapping or clanking sound which becomes louder as pressure increases is audible However if the motor noise suddenly becomes much louder contact Dionex Technical Support for assistance DSP card current limit has been exceeded Turn on the GS50 by pressing the main power switch Check the DSP digital signal processing card in slot 1 of the GS50 electronics chassis see Figure 2 5 Note the three small LEDs in the upper left corner of the DSP card bulkhead if the bottom LED 1s flashing in time with the pump strokes the current limiter is being activated The built in current limiter protects the motor and motor drive As the pump motor ages it is normal for the current limit to be activated more frequently However if th
72. ed the pump automatically turns on To exit the priming mode and return to the previously selected flow rate press Prime again or press Off On to turn off the pump motor See Section B 2 7 for complete priming instructions Inserts a new timed step into a method This button functions only when the cursor is in a TIME field on either the METHOD or METHOD events screen 1 Move the cursor to the TIME field and press Insert The new step is added after the cursor position Parameter values in the new step are blank 2 Fill in the time value and press Enter or a cursor arrow button Note If you move the cursor out of the TIME field before entering a time value the inserted step disappears because it is incomplete 3 Insert steps in any order When you press Enter the GS50 Moduleware organizes steps in the correct chronological order Table 2 1 GS50 Front Panel Buttons 2 3 GS50 Gradient Pump Button Function Delete performs these functions Delete e Cancels an entry that is in progress and restores the previous value Returns a field to its default value 1f no entry 1s in progress Blanks the parameter value for a method step On the METHOD screen move the cursor to a step entry field press Delete once and move the cursor out of the field The field remains blank indicating that the value entered for the previous step is still in effect Deletes a method step On the METHOD screen move the cursor to
73. egas eluents daily following the instructions below and store eluents in pressurized reservoirs Manual Degassing of Eluents l 2 Doc 031612 03 10 03 Prepare the eluent required for the application Pour the eluent into a vacuum flask and attach the flask to a vacuum pump or water aspirator Vacuum degas the eluent for 5 to 10 minutes by shaking or sonication Remove the flask from the vacuum Do not allow water to flow from the aspirator back into the flask Pour the degassed eluent into a pressurizable reservoir Do not shake the eluent 3 1 GS50 Gradient Pump 3 2 CAUTION 6 Install end line filters on each eluent line see Section 3 1 2 7 Pressurize the reservoirs see Section 3 1 3 Filter Eluents Before beginning an analys s always filter eluents Filtering removes small particulates in the eluent that may contaminate the pump check valves or the eluent proportioning valves and cause erratic flow rates or loss of prime All Dionex pressurizable reservoir Ship Kits include end line filters for this purpose Before installation thoroughly rinse the end line filters P N 045987 with deionized water to remove any loose particles Install a filter on the end of each eluent line inside the reservoir Verify that the end of each filter extends to the bottom of the reservoir and that each filter is submerged in eluent This prevents air from being drawn through the line For more details refer to t
74. ent B for 5 minutes 7 Move the cursor to the next line Enter 20 10 in the TIME field Move the cursor to the A field and enter 100 8 Move the cursor to the SAVE TO field and press Enter to save the method to memory Figure 3 9 illustrates the completed method METHOD EDIT 2 SAVE TO 2 LIMITS 0 5000 PSI TIME A B C D C V FLOW 5 00 100 0 L 2 00 gt gt 15 00 20 0 80 0 20 00 20 0 80 0 gt 20 10 100 0 gt Help prompt Figure 3 9 Linear Gradient Method Completed 3 18 Doc 031612 03 10 03 3 Operation and Maintenance 9 Press MENU and Enter to go to the MAIN screen Enter 2 in the METHOD field Press Reset to reset the method to the INIT step if necessary 10 Ifthe pump motor is off press Off On to start the pump 11 Ifthe method clock is in hold press Hold Run to start the method running When the method reaches the last step in the method TIME 20 10 the pump continues to pump isocratically until the clock is reset 3 4 3 Curved Gradient Method Example Here is a summary of the curved gradient method example steps e Create Method 3 to begin under isocratic conditions with 100 eluent A at 1 5 mL min e Hold this for 3 minutes TIME 3 00 e Begin a 10 minute convex segment in which the amount of eluent B is increased and the amount of eluent A is decreased until the mixture is 100 eluent B TIME 13 00 e Hold this for 2 minutes TIME 15 00 Figure 3 10 illustrates the gradie
75. et them air dry If a reservoir still appears dirty or if there is a slimy film on the interior follow the cleaning procedure in the Pressurizable Reservoir Installation Instructions Doc 031612 03 10 03 3 23 GS50 Gradient Pump Weekly e Check the junctions between the pump heads and the pump casting for evidence of liquid leaks If the piston seal wash tubing is not connected check the drain tubes at the rear of the pump heads for evidence of moisture Normal friction and wear may gradually result in small liquid leaks around the piston seal If unchecked these leaks can gradually contaminate the piston housing causing the pump to operate poorly If leaks occur replace the piston seals see Section 5 2 e Check the end line filters and change if needed When new end line filters are pure white If the system is in continuous operation change the filters weekly or whenever they become discolored Replace the filters more often if you notice bacterial buildup or if the mobile phase does not contain solvent NOTE It is especially important to regularly replace end line filters when using aqueous eluents which may contaminate the filter with bacteria or algae Although the contamination may not be visible it can cause flow restrictions to the pump Every Six Months e Replace the main piston seals see Section 5 2 e Replace the piston rinse seals and O rings see Section 5 2 Yearly e Replace the backup seals see Sectio
76. fin Verwenden Sie den 2 Liter Plastikbehalter Bestell Nr 044129 nicht zum Offline Vakkum Entgasen von Eluenten Der Behalter ist dafur nicht ausgelegt Piston Seal Wash Connections Optional The GS50 includes a piston seal wash assembly intended to continuously rinse the back of the piston seals to remove salt crystals and prolong the life of the seals Follow the steps below to set up the seal wash function 1 Fill a flush bottle with either deionized water or a combination of deionized water and 10 or 20 isopropyl alcohol Isopropyl alcohol will inhibit bacterial growth in the water 2 Place the flush bottle above the GS50 During operation gravity will maintain flow through the pump heads 3 The flush solution is carried to the piston seal wash assembly and out to waste via clear 1 5 mm 0 06 in ID tubing P N 055847 Locate this tubing in the GS50 Ship Kit and cut the tubing into three unequal pieces in the following lengths e 117 cm 46 in e 152 cm 60 in e 15 cm 6 in Doc 031612 03 10 03 B Installation 4 Install the tubing as follows see Figure B 3 a Push the 117 cm 46 in piece of clear tubing onto the drain tube on the bottom of the secondary pump head Place the free end of this tubing in the flush bottle Make sure the end of the tubing is near the bottom of the bottle b Push the 152 cm 60 in piece of clear tubing onto the drain tube on the top of the primary pump head This is the
77. fuses rated 3 15 amps P N 054745 Dionex recommends always replacing both fuses at the same time Fuse Holder pussa See Locking Spring i Insert Fuse Holder screwdriver Side View and twist to release each side Main Power Receptacle Figure 5 6 Main Power Fuse Holder 5 14 Doc 031612 03 10 03 5 Service 4 Reinsert the fuse holder into its compartment Apply enough pressure evenly against the holder to engage the two locks when both locks are engaged the holder is flush against the panel 5 Reconnect the main power cord and turn on the power 5 7 Changing the GS50 Delay Volume Optional The GS50 is plumbed at the factory to ensure a low delay volume 400 uL Section 5 7 explains how to modify the GS50 plumbing to obtain the standard delay volume of 800 uL Increasing the delay volume will improve mixing noise at flow rates of 1 0 mL min and above The replumbing procedure requires two simple changes e Replacement of the tubing assembly installed between the eluent proportioning valve and the primary pump head e Replacement of the gradient mixer The standard delay volume conversion kit P N 056968 contains all the parts required for this procedure Standard Delay Volume Conversion Kit Part Number Item Quantity 055878 Tubing Assembly 0 8 mm 0 030 in ID 1 043275 10 32 Fitting Bolt 2 043276 Ferrule Fitting 2 054044 GM 5 Gradient Mixer l 055860 Component Clip 3 4 inch 1 0
78. ginal method save the modified method to a new number This is the basic procedure for editing a method 1 Go to the METHOD screen In the EDIT field enter the number of the method to be modified 2 Make the required changes e To change a parameter position the cursor in the field and enter the new parameter e To adda method step move the cursor to any TIME field and press Insert or move the cursor to the TIME field below the last step and enter the elapsed time at which to start the new step When you press Enter or a cursor arrow button the Moduleware automatically moves the new step to the correct chronological position Continue entering parameters for the new step e To delete a method step move the cursor to the TIME field of the step and press Delete twice 3 When you finish editing move the cursor to the SAVE TO field Press Enter to save the changes to the current method number or enter a new method number and press Enter If the modified method is currently running the changes are immediately incorporated in the run and executed at the programmed time unless the modified event has already been executed If this is the case press Reset to restart the method at the INITial conditions and run the updated version of the method Doc 031612 03 10 03 3 13 GS50 Gradient Pump 3 3 4 Deleting a Method To delete an entire method move the cursor to the TIME field of the INIT step on the METHOD screen and pres
79. h 9 normalized for 0 to 100 for 10 minutes e Any consecutive method steps that specify identical eluent compositions will generate an isocratic segment regardless of the curve number selected e A step gradient change is a steep linear change from one eluent to another A step change occurs automatically if the time interval between two steps is less than 0 1 minute 6 seconds Figure 2 10 Eluent Composition Profile for Curves 1 9 2 24 Doc 031612 03 10 03 3 Operation and Maintenance 3 1 Getting Ready to Run NOTE The GS50 Gradient Pump is designed to perform ion chromatography and BioLC applications only and should not be used for any other purpose If there is a question regarding appropriate usage contact Dionex 3 1 1 Degas Eluents Eluent quality significantly affects the performance of the GS50 To ensure optimal pump performance observe these precautions Dionex strongly recommends vacuum degassing all eluents This helps prevent bubbles caused by eluent outgassing from forming in the eluent proportioning valves pump heads and detector cell Degassing is especially important when combining aqueous and nonaqueous components for example water and acetonitrile The optional vacuum degas assembly provides programmable online degassing of up to four eluents see Section 2 5 The vacuum degas assembly must be installed in the GS50 at the factory If your GS50 does not include this option manually d
80. h installation Periodically refill the flush bottle and empty the waste container as required B 10 Doc 031612 03 10 03 B Installation B 2 Priming the Pump Section B 2 7 describes three ways to prime the pump The first two procedures Priming with a Syringe and Priming with the Prime Button are standard procedures If these are ineffective try the third procedure Priming with Isopropyl Alcohol Priming with a Syringe NOTE Priming with a syringe is recommended after changing eluents or when eluent lines are empty 1 Goto the MAIN screen see Section C 1 2 Check that the operating fields are set to LOCAL and DIRECT CNTRL If REMOTE or METHOD is currently selected move the cursor to the appropriate field press Select A or Select V to toggle to the correct setting and press Enter or a cursor arrow button 2 Move the cursor to the desired eluent Enter 100 to select 100 of the eluent and press Enter or a cursor arrow button 3 Connect a 10 mL syringe P N 054578 to the luer port in the priming valve on the primary pump head see Figure B 4 Secondary ak Pump Head N AL p sl Primary Pump Head 10 mL Syringe CS N N P N 054578 mind Figure B 4 Priming the Pump Doc 031612 03 10 03 B 11 GS50 Gradient Pump 10 11 Open the waste valve on the secondary pump head see Figure B 4 by turning the knob one quarter to one half turn counterclockwise Opening the valve directs the eluent f
81. he Pressurizable Reservoir Installation Instructions Pressurize Eluent Reservoirs NOTE Although the GS50 does not require pressurized reservoirs Dionex recommends pressurizing the reservoirs if eluents are manually degassed see Section 3 1 1 or if eluents are sensitive to carbonate NaOH contamination For more details refer to the Pressurizable Reservoir Installation Instructions 1 Install the pressurizable reservoirs above the pump 2 Install a regulator P N 046594 on the gas supply line to the reservoirs 3 Turnon the gas supply and adjust the pressure to 55 kPa 8 psi Never pressurize eluent reservoirs above 69 kPa 10 psi Pressurizing reservoirs above this limit can cause the reservoir to explode Doc 031612 03 10 03 3 Operation and Maintenance Ne mettez jamais les r servoirs d luants sous une pression sup rieure a 69 kPa 10 Ib po MISE EN GARDE Setzen Sie den Eluentbeh lter auf keinen Fall einem Druck ber 69 kPa aus VORSICHT 3 1 4 Start Up l 2 Doc 031612 03 10 03 Turn on the main power switch The POWER UP screen appears briefly see Figure 2 2 and is then replaced by the MAIN screen see Figure 2 3 NOTE If a power up diagnostic test fails the DIAGNOSTIC TEST screen appears instead of the MAIN screen See Section C 2 5 if this occurs If one of the following conditions applies prime the pump see Section B 2 7 e The eluent has been changed e The eluent
82. he RDY field to CAL and press Enter c The degas pump should turn on and run for about 2 minutes if it does not see Section 4 6 2 Ifthe GS50 does not contain a vacuum degas assembly degas eluents manually see Section 3 1 1 and reprime the pump see Section B 2 7 Doc 031612 03 10 03 4 1 GS50 Gradient Pump 4 2 Eluents insufficiently degassed If the GS50 contains a vacuum degas assembly the degas pump automatically turns on periodically Go to the DEGAS OPTIONS screen see Section C 1 6 and increase the frequency For example run the degas pump for 30 seconds at 2 minute intervals If eluents are manually degassed degas them for longer periods of time Pump out of prime end line filter is dirty or clogged 1 When new end line filters P N 045987 are pure white If the system is in continuous operation replace the end line filters weekly or whenever a filter becomes discolored Replace filters more often if bacterial buildup is visible or if the mobile phase does not contain solvent See the Pressurizable Reservoir Installation Instructions for more information about end line filters NOTE It is especially important to regularly replace end line filters when using aqueous eluents which may contaminate the filter with bacteria or algae The bacterial buildup may not be visible 2 Reprime the pump see Section B 2 7 Pump out of prime blockages in inlet tubing Kinked or clogged tubing causes the pump to be
83. he interior following the cleaning procedure in the Pressurizable Reservoir Installation Instructions 2 Replace all end line filters P N 045987 see Section 3 1 2 3 Prepare new stock solution 4 Prepare fresh mobile phase To ensure their purity prepare all mobile phases with spectro grade solvents reagent grade chemicals and ASTM Type I or better filtered and deionized water e Problem unrelated to the pump 1 The mobile phase was prepared at the wrong concentration or prepared with impure chemicals solvents or water To ensure their purity prepare all mobile phases with spectro grade solvents reagent grade chemicals and ASTM Type I or better filtered and deionized water 2 The column may be the source of the problem Refer to the column manual for troubleshooting assistance 3 The injection valve may be the source of the problem Refer to the chromatography module operator s manual for troubleshooting assistance 4 14 Doc 031612 03 10 03 4 Troubleshooting 4 12 Noisy Baseline Eluents not filtered Install an end line filter P N 045987 on the end of each eluent line inside the reservoir Verify that the end of each filter extends to the bottom of the reservoir and that each filter is submerged in eluent For more details see Section 3 1 2 Pump not properly primed Prime the pump see Section B 2 7 Pressure ripple needs to be calibrated Go to the CALIBRATION MENU select the PRESSURE RIPPLE CA
84. he pistons thereby causing the main piston seals to wear out prematurely and allow leaks To use the seal wash feature an external water source must be connected See Section B 2 6 for connection instructions For continued protection of the pump replace the piston rinse seal and the O rings n the seal wash assembly see Section 5 2 every 6 months or whenever you replace the main piston seals for the GS50 Proportioning Valves Mobile phase flows from the eluent reservoirs through the vacuum degas chambers if the optional vacuum degas assembly is installed and into the four way proportioning valve assembly see Figure 2 6 Programmed percentages of each eluent are proportioned in the four valves Pressure Transducer Flow exiting the secondary pump head is directed to the pressure transducer see Figure 2 6 which measures the system pressure The DSP digital signal processor firmware precisely controls the pump motor speed to ensure flow rate accuracy and to maintain constant flow and constant pressure Flow output from the pressure transducer continues to the gradient mixer see Section 2 4 3 From there it is directed to the remainder of the chromatography system the injection valve column and detector Gradient Mixer A GM 4 Gradient Mixer P N 049136 is installed between the pressure transducer and the injection valve see Figure 2 6 The mixer helps to ensure that proportioned eluents are mixed thoroughly N
85. hether the degas pump is turned on or off When the degas pump turns on it runs for the selected CYCLE DURATION time see below ALWAYS OFF The degas pump is always off ALWAYS ON The degas pump is always on this setting is reserved for test purposes by Dionex Service Representatives The length of time 2 to 5 minutes the degas pump runs after the GS50 power is turned on The length of time 0 to 120 seconds the degas pump runs during each cycle C 9 GS50 Gradient Pump TIME BETWEEN The length of time 1 to 99 minutes between degas CYCLES cycles NOTE The DEGAS PUMP CALIBRATION AND STATUS screen displays the degas threshold value see Section C 3 3 The GS50 monitors the vacuum degas reading at 1 minute intervals If the reading is at or below the calibration threshold value the following message is displayed LOW VACUUM ALARM Check DEGAS OPTIONS settings or refer to service manual If this message appears increase the CYCLE DURATION time and or decrease the TIME BETWEEN CYCLES If this does not eliminate the problem contact Dionex Technical Support C 10 Doc 031612 03 10 03 C User Interface C 1 7 Module Setup Use the MODULE SETUP screen to select display options MODULE SETUP DISPLAY PANEL BACKLIGHT MEDIUM KEY ACTUATION SOUND ON ENTRY ERROR SOUND ON HELP LANGUAGE ENGLISH Help prompt Figure C 8 Module Setup Screen DISPLAY PANEL Sets the brightness of the dis
86. ion a red LED indicates a power fault When a power fault occurs the GS50 enters a diagnostic state and inhibits all other controls until the fault is corrected If the LED is red turn off the power for a few seconds and then turn it back on 4 4 Doc 031612 03 10 03 4 Troubleshooting e High pressure limit was tripped the following message is displayed High Pressure Limit Violation 1 The high pressure limit setting may be too low As columns age their backpressure increases and it may be necessary to compensate for this by increasing the high pressure limit If the column is the source of the high backpressure clean the column as instructed in the column manual If this does not eliminate the problem replace the column 2 Observe a run to see whether the high pressure limit is triggered when injection occurs if it 1s the injection valve may be causing a blockage This indicates that the rotor seal or stator may need replacement Refer to the operator s manual for the chromatography module for instructions 3 Make sure that no tubing is plugged or overtightened To determine the source of the high backpressure isolate segments of the flow path Remove the pump outlet tubing at the injection valve b Press Off On to turn on the pump Set the flow rate to 1 0 mL min and record the backpressure c Oneata time reconnect each component of the flow path If reconnecting a component causes a sharp increase in backpressure
87. ive and negative edges are reversed in function Normal negative going Inverted positive going Figure D 4 TTL and Relay Input Signal Modes D 4 Doc 031612 03 10 03 D TTL and Relay Control D 3 TTL and Relay Connections The TTL relay connector strip is on the electronics chassis behind the upper door of the GS50 enclosure see Figure D 1 Each 2 pin connector includes a signal pin and a ground pin l Locate the twisted pairs of wires P N 043598 and 2 pin connector plugs P N 921019 provided in the GS50 Ship Kit P N 061222 Attach a 2 pin plug to each end of the twisted pair of wires to be connected NOTE The signal wire must be on the top of each plug and the ground wire must be on the bottom of each plug Connect these plugs to the TTL or relay connectors on the GS50 and the other instrument s as needed for the application Be sure to connect signal wires to signal pins and ground wires to return pins If necessary remove wires from the 2 pin plugs and reinsert them in the correct positions Section D 3 1 shows example connections Route the wires from the GS50 electronics chassis through the upper chase to the rear panel Doc 031612 03 10 03 D 5 GS50 Gradient Pump D 3 1 Example Connections Figure D 5 shows an example of TTL relay connections for a Dionex system connected to an AS40 Automated Sampler Refer to the AS40 operator s manual for details Detector AS40
88. lass Reservoir shatterproof plastic coating 2 Liters Plastic Reservoir 1 Liter Plastic Reservoir 2 Liters Filter End Line Pressure Regulator for gas supply line to reservoirs Fitting Plug 10 32 Syringe 10 mL 3 15 Amp Fuse Fast Blow IEC 127 2 Pin TTL Relay Connector Twisted Wire Assembly Quantity en l l 2 m 8 ft Doc 031612 03 10 03 A Aborting a running method 3 14 Actuator for main power switch 2 1 Air pressure requirements 3 2 Air solenoid valves 2 10 Annual maintenance 3 24 Arrow buttons 2 2 2 5 Automated control of GS50 1 1 B Backlight Adjusting the brightness C 11 Time in use C 17 Backup seals Maintenance 3 24 Blank field in method step 3 10 Button descriptions 2 4 2 5 C Cables DX LAN B 6 Front panel 60 pin ribbon cable 2 11 LC10 LC20 leak sensor B 5 LC10 LC20 solenoid valve B 5 LC30 serial interface B 4 Power cord 5 14 B 3 Calibration Menu C 20 Calibration procedures Degas assembly C 22 Flow rate C 23 Leak sensor C 21 Pressure offset C 24 Pressure ripple 3 5 3 6 Cascading hubs B 6 Caution icon 1 4 1 5 Doc 031612 03 10 03 Index Check valves 2 15 Cause of pressure fluctuations 4 2 Cleaning procedure 5 1 5 3 Maintenance 3 24 Malfunction 4 14 Replacement procedure 5 1 5 3 Time in use C 17 Chromatography modules Connections to electronics chassis B 4 Chromeleon software 1 1 2 10 Connect command 2 21 Locked Rem
89. lded twisted pair cable Do not substitute a cable of an inferior grade Failure to use the correct cable will cause the pump to lose communication with the host computer 3 Connect the other end of the cable into the 1OBASE T DX LAN connector on the GS50 rear panel see Figure B 1 4 Connect a IOBASE T cable P N 960281 from a IOBASE T port on the hub to the IOBASE T port on the host computer s internal DX LAN card If the connection is via port 8 on the hub set the Normal Uplink push button to Normal NOTE For installation instructions for the internal DX LAN card refer to Installing the Chromeleon IC System Document No 031883 or Installing the Dionex PeakNet 6 System Document No 031631 When using 10BASE T cabling you must install a hub If you simply plug the 10BASE T cable from the pump into the connector on the host computer s DX LAN card the connection will either not work or Will be unreliable Cascading Hubs Cascading connecting two or more hubs together via their 1OBASE T ports increases the number of ports or the number of users supported on the network For instructions on how to cascade hubs refer to the installation guide shipped with the hub Doc 031612 03 10 03 B Installation B 2 4 Waste Lines Two waste lines are installed at the factory and routed to the GS50 rear panel e One waste line exits the waste valve on the secondary pump head e One waste line exits the leak drain A third w
90. leon or PeakNet 6 software via the DX LAN When the GS50 is controlled by Chromeleon or PeakNet 6 connecting the pump to the timebase automatically selects the Locked Remote operating mode In this mode time based steps for the software are executed on the server and the software then sends operating commands directly to the pump All operating changes from the GS50 front panel are disabled To return the GS50 to Local mode clear the Connect check box on the GS50 control panel in Chromeleon or PeakNet 6 To return to remote mode select the Connect check box 2 8 2 Method Front Panel Control In Method control commands are executed according to the time based steps programmed in a method Each step specifies the eluent composition and flow rate delivered by the pump at a given time The selected eluent mixture can be delivered isocratically or as a multistep linear or curved gradient As the method runs the GS50 calculates the changes in eluent composition required to deliver a gradient from one method step to the next or to match the selected curve Doc 031612 03 10 03 2 21 GS50 Gradient Pump Methods are created saved and edited on the METHOD screen see Figure 2 9 See Section 3 3 for programming instructions METHOD EDIT 5 SAVE TO 6 LIMITS 0 5000 PSI TIME A B C D C V FLOW INIT 25 0 25 0 25 0 25 0 L 2 00 gt gt 0 00 100 0 123 45 10 0 22 2 32 2 36 5 5 L gt 345 67 17 2 19 6 33 2 30 0 gt Help prompt
91. line is new empty e The eluent line contains air or e The pressure display is unstable Select the flow rate required for your application Press Off On to start the pump flow Check the pressure reading on the MAIN screen The display is updated once per pump cycle a variation of more than 3 from one pressure reading to the next indicates that the pump is out of prime See Section 4 1 for conditions that cause the pump to lose prime NOTE After starting the pump or changing the flow rate wait at least 5 minutes longer for flow rates below 1 0 mL min before beginning an analysis This 3 3 GS50 Gradient Pump allows the digital signal processor DSP program to stabilize the flow rate 3 1 5 Selecting the Pressure Limits The high and low pressure limits automatically stop the pump when a system malfunction occurs such as overpressurization caused by a blockage or low pressure caused by a leak downstream from the pump e When pump operation is being controlled by Chromeleon or PeakNet 6 select the pressure limits from the software e When the pump is running under Local Direct control enter the pressure limits on the DETAIL screen see Figure 3 1 e When the pump is running under Method control enter the pressure limits on the METHOD screen see Figure 3 5 The limits are set in the INIT step of the Method and remain the same throughout the analysis When a limit trip stops the pump the method clock immediately st
92. low path to waste and eliminates backpressure NOTE Ifthe eluent is pressurized liquid flows into the syringe as soon as the priming valve is opened in Step 5 Do not pressurize above 55 kPa 8 psi Open the priming valve by turning it one quarter to one half turn counterclockwise Press Off On to turn on the pump motor If the eluent is not pressurized loosen the cap on the eluent reservoir or make sure there is a vent to the reservoir Draw the syringe back to begin pulling eluent through the flow path It may take several syringe draws to remove all air or previous eluents from the tubing NOTE If the vacuum degas assembly is installed draw an additional 17 mL of eluent through the pump head Return to Step 2 Select another eluent and repeat the priming procedure After priming the manifold with each eluent close the priming valve Do not overtighten Press Off On to turn off the pump motor The pump is now ready for operation Doc 031612 03 10 03 B Installation Priming with the Prime Button l Go to the MAIN screen see Section C 1 2 Check that the operating fields are set to LOCAL and DIRECT CNTRL If REMOTE or METHOD is currently selected move the cursor to the appropriate field press Select A or Select V to toggle to the correct setting and press Enter or a cursor arrow button 2 Set the eluent to 100 of the line to be primed Press Enter or a cursor arrow button 3 Check that the priming v
93. ly the field indicates YES To check the vacuum chamber pressure go to the CALIBRATION MENU and select the DEGAS PUMP CALIBRATION AND STATUS screen see Section C 3 3 2 16 Doc 031612 03 10 03 2 Description 2 6 Eluent Reservoirs NOTE Dionex strongly recommends vacuum degassing and filtering all eluents to help prevent bubbles contaminants and particles from passing through the system Filtering eluents also helps reduce baseline noise and ensure system stability NOTE Although the GS50 does not require pressurized reservoirs Dionex recommends pressurizing the reservoirs if eluents are manually degassed see Section 3 1 1 or if eluents are sensitive to carbonate NaOH contamination For more details see the Pressurizable Reservoir Installation Instructions Document No 034581 The following pressurizable reservoirs are available from Dionex e liter glass reservoir with shatterproof plastic coating P N 044126 e 2 liter glass reservoir with shatterproof plastic coating P N 044127 e liter plastic reservoir P N 044128 e 2 liter plastic reservoir P N 044129 Do not use the 2 liter plastic reservoir P N 044129 for offline vacuum degassing of eluents The reservoir was not designed for this purpose CAUTION N utilisez pas le r servoir en plastique de 2 litres N P 044129 pour le d gazage a vide hors ligne d luants Le r servoir n a pas t concu a cette fin MISE EN GARDE Verwenden Sie den
94. m DIRECT CNTRL to METHOD 3 4 1 Isocratic Method Example Specify an isocratic mixture of 60 eluent A 25 eluent B and 15 eluent C to be pumped at 1 0 mL min Figure 3 3 illustrates the isocratic profile for this example Figure 3 6 illustrates the METHOD screen as it appears when the example is set up METHOD EDIT 1 SAVE TO 1 LIMITS 0 5000 PSI TIME A B C D C V FLOW INIT 60 0 25 0 15 0 0 00 Help prompt Figure 3 6 Method Screen Isocratic Run Example 1 Go to the METHOD screen and enter a method number 1 for example in the EDIT field The screen automatically changes the number in the SAVE TO field to the number of the method being edited e If Method 1 already exists and you want to retain it enter a new unused method number in the EDIT field e If Method 1 already exists and you want to delete it move the cursor to TIME INIT and press Delete twice Doc 031612 03 10 03 3 15 GS50 Gradient Pump 2 Move the cursor to the A field and enter 60 Move to the B field and enter 25 Move to the C field and press Enter 15 is automatically filled in to make the eluent percentages equal 100 Ignore the C Curve and V Valve fields Move to the FLOW field and enter a flow rate of 1 00 3 Move the cursor to the SAVE TO field and press Enter to save the method 4 Goto the MAIN or DETAIL screen enter the new method number in the METHOD field 1 in this case and press Enter If the pump motor is off p
95. m the host computer via the DX LAN See Section 2 8 1 for a description of Locked Remote mode With TTL input a controlling device for example an integrator or another module sends TTL signals to the pump The TTL input signals can be used to trigger the following functions turn the pump motor on off and set the method clock to hold run All other pump operating parameters must be set locally from the control panel See Appendix D for more information about TTL control To select the operating mode l Go to either the MAIN or DETAIL screen 2 The operating mode field displays either LOCAL or REMOTE see Figure 2 8 To change the mode move the cursor to this field press Select A or Select V to toggle to the desired mode and press Enter or a cursor arrow button For TTL input control select Local mode 100 0 A m 2000 ps 0 0 B a 2 00 mumin 0 0 20 10 27 min 0 0 D LOCAL METHOD 2 Help prompt Figure 2 8 Main Screen Doc 031612 03 10 03 2 19 GS50 Gradient Pump In addition to the operating modes two control modes are available from the GS50 front panel In Direct control commands are executed immediately when entered Because there is no time based program the method clock is not used and the Hold Run and Reset buttons do not operate In Method control commands are executed according to the timed steps in a method programmed from the front panel See Section 2 8 2 for details about Method control
96. mbrane pores e Shut down the GS50 by turning off the main power switch Doc 031612 03 10 03 3 25 GS50 Gradient Pump 3 26 Doc 031612 03 10 03 4 Troubleshooting This chapter is a guide to troubleshooting minor problems that may occur during operation of the GS50 Gradient Pump First turn to the section of this chapter that best describes the operating problem There possible causes of the problem are listed in order of probability When necessary you will be referred to other product manuals for additional information These manuals are usually located on the Dionex Reference Library CD ROM P N 053891 If you are unable to resolve a problem contact Dionex Technical Support In the U S call 1 800 346 6390 Outside the U S call the nearest Dionex office 4 1 Pressure Fluctuations Between Pump Heads The GS50 display updates the pressure readout once per second Excessive pressure variation more than 3 difference from one pressure reading to the next or a low pressure reading indicates a problem e Pump out of prime there is no eluent 1 Refill the eluent reservoirs Make sure that each eluent line extends to the bottom of the reservoir 2 Reprime the pump see Section B 2 7 e Pump out of prime eluents are improperly degassed 1 Ifthe GS50 contains a vacuum degas assembly test the degas pump a Go to the DEGAS PUMP CALIBRATION AND STATUS screen see Section C 3 3 b Press a Select button to toggle t
97. minute Doc 031612 03 10 03 C 3 GS50 Gradient Pump MIN Displays the total elapsed time that the method clock has been running When a new elapsed time is entered the method settings corresponding to the new time take effect LOCAL Selects the operating mode REMOTE i Local mode is used when full control from the GS50 front panel is needed Remote mode is used when Chromeleon or PeakNet 6 is controlling the GS50 In this mode operation from the GS50 front panel is disabled There are two ways to clear Remote mode select the Disconnect command from the software or turn off the pump power briefly and then turn it on again METHOD In Method control enter the method number to run in this field C 4 Doc 031612 03 10 03 C User Interface C 1 3 Detail Screen Use the DETAIL screen to e Display detailed information about the status of GS50 operating parameters This screen displays all the information shown on the MAIN screen see Section C 1 2 as well as some additional parameters e Set several parameters for Direct Control operation DETAIL SCREEN RLY1 0 100 0 0 0 0 0 0 0 CURVE 5 A 2125 PSI RLY2 1 B 2 00 mL MIN TTL1 O 7o C INJECT SAMPLE TTL2 1 D COLUMN A LIMITS 0 5000 PSI REMOTE METHOD 5 10 25 MIN Help prompt RLY1 RLY2 TTL1 TTL2 CURVE LIMIT Doc 031612 03 10 03 Figure C 4 Detail Screen Provides Relay and TTL control of other devices In Direct control select
98. n 2 23 INIT conditions 3 11 Isocratic eluent method 2 23 3 15 Linear gradient method example 3 16 Method control 3 9 Preparing to run 3 1 Priming B 11 B 14 Index 6 Programming the pump 3 15 Running a method 3 12 Selecting pressure limits 3 4 Shutdown 3 25 Operational screens C 2 Outlet check valve 2 15 P PeakNet 6 software 1 1 2 10 Connect command 2 21 Locked Remote mode 2 19 2 21 PEEK components 5 2 Physical description Control panel 2 2 Degas assembly 2 16 Pressure transducer 2 14 Rear panel 2 18 Physical specifications A 1 Piston seal wash assembly 2 14 3 23 Connections B 8 Maintenance 3 24 Piston seals Cause of pressure fluctuations 4 2 Maintenance 3 24 Replacement procedure 5 4 5 7 5 8 Sources of damage 3 25 Time in use C 17 Pistons Maintenance 3 23 3 25 Replacement procedure 5 9 Power cord 5 14 B 3 Power fault 2 11 4 4 Power requirements A 1 Power supply 2 11 Power switch 2 1 Power Up screen C 15 Pressure limits 3 4 Pressure offset calibration C 24 Pressure Offset Calibration screen C 24 Pressure ripple A 2 Pressure ripple calibration 3 5 3 6 Pressure Ripple Calibration screen C 25 Doc 031612 03 10 03 Pressure Statistics screen C 16 Pressure transducer 2 14 2 15 4 5 Pressure units C 12 Priming 2 3 B 11 Pump is out of prime 4 2 Reasons pump needs priming 4 1 With a syringe B 11 With isopropyl alcohol B 14 With the Prime
99. n between pistons is characteristic of dual piston pumps and is not a defect Before beginning routine operation with the GS50 calibrate the pressure ripple to optimize pump operation The calibration procedure forces the pump to learn the specific operating parameters for your application this will minimize both baseline noise and the pressure ripple To calibrate the pressure ripple 1 Check that all normal operating conditions for the application are in effect the separator and guard columns are installed the appropriate flow rate 1s selected etc 2 Press Off On to start the pump flow Run the pump for at least 10 minutes before going on to Step 3 and starting the calibration Doc 031612 03 10 03 3 5 GS50 Gradient Pump 3 Go to the CALIBRATION MENU and select the PRESSURE RIPPLE CALIBRATION screen see Figure 3 2 PRESSURE RIPPLE CALIBRATION CALIBRATION READY DURATION xxx xx min Help prompt Figure 3 2 Pressure Ripple Calibration Screen 4 Move the cursor to the CALIBRATION field Toggle the field from READY to CALIBRATE and then press Enter to start the calibration At a flow rate of 1 0 mL min it takes about 13 minutes to run the calibration sequence The DURATION field reports how much time remains until the calibration is finished 5 When the calibration sequence is complete press Menu twice to exit the PRESSURE RIPPLE CALIBRATION screen and return to the MENU of SCREENS NOTE For more
100. n 5 2 Inspect the check valves annually replace as needed see Section 5 1 Inspect the pistons annually replace as needed see Section 5 3 3 24 Doc 031612 03 10 03 3 Operation and Maintenance 3 6 Shutdown e Rinse the pump pistons before and after daily operation to prevent buildup of salt crystals or other contaminants that can damage the piston seal Never store the pump with salt buffers in the flow path e Before shutting down the GS50 for 3 days or more flush the system with deionized water to prevent contaminants from building up If this is not possible maintain a continuous rinse through the system until normal operation is resumed In addition set the flow rate to 0 05 mL min and set all four eluent percentages to 25 to ensure that the proportioning valve assembly is flushed Flushing the proportioning valves is extremely important if the eluents combine salt or base and solvent If salt precipitates in the valves it can seriously damage the valve diaphragms If this happens the entire proportioning valve assembly must be replaced see Section 5 5 CAUTION e Before shutting down the GS50 for more than 3 days reduce the pressure on the eluent reservoirs to about 21 kPa 3 psi e Before shutting down the GS50 for 4 weeks or more Dionex recommends thoroughly flushing any chemicals out of the vacuum degas assembly tubing if installed with deionized water This helps prevent crystallization in the me
101. nect the electrical connector for the proportioning valve assembly from the distribution card a Using a Phillips screwdriver remove the screw that secures the mechanical chassis drawer to the enclosure The screw is on the lower left side of the chassis below the secondary pump head see the label on the inside of the lower door b Pull out the mechanical chassis drawer a few inches c The distribution card is located on the top of the mechanical chassis Locate the electrical connector labeled VALVES at the front of the card Disconnect the connector from the card Disconnect the liquid line from the outlet of the proportioning valve assembly see Figure 5 5 Loosen the screws securing the proportioning valve assembly to the bulkhead Pull the valve assembly forward and disconnect the eluent lines on the inlet Remove the valve assembly from the bulkhead Thread the bundle of electrical lines from the new valve assembly P N 046203 through the bulkhead and up to the distribution card Connect the valve electrical connector to the VALVES connector on the distribution card Doc 031612 03 10 03 5 Service Gradient Mixer Proportioning Valve Assembly Outlet Figure 5 5 Eluent Proportioning Valve Assembly 7 Connect the eluent lines to the new valve assembly Make sure eluent lines A through D are in the appropriate valve ports Connect eluent line A to the port marked 1 eluent B to the port marked 2 and so on
102. ng see Section C 3 6 Table 2 1 GS50 Front Panel Buttons Continued 2 5 GS50 Gradient Pump Button Function Pressing a numeric button enters a numeric value into the current entry 1 i field When a menu is displayed pressing the numeric button that corresponds to a menu option displays the screen Enter performs these functions Enter e Saves and or executes changes made in entry fields except on the METHOD screen On the METHOD screen pressing Enter saves entries to an edit copy To save editing changes to a permanent method move the cursor to the SAVE TO field enter the method number and press Enter e When a menu is displayed pressing Enter opens the highlighted screen Table 2 1 GS50 Front Panel Buttons Continued 2 6 Doc 031612 03 10 03 2 Description 2 1 2 Display Screens When the pump powers up and passes all diagnostic tests the POWER UP screen see Figure 2 2 is displayed briefly followed by the MAIN screen see Figure 2 3 If a diagnostic test fails the DIAGNOSTIC TEST screen appears instead of the MAIN screen see Section C 2 5 for details GS50 GRADIENT PUMP DSP MODULEWARE REV nn nn BIOS nn nn BIOS REV nn nn MAIN nn nn DX LAN ID nnnnnn Help prompt Figure 2 2 Power Up Screen 100 0 JA Em r 2000 psi 0 0 B 2 00 mumin 10 27 m n 0 0 C 0 0 D LOCAL METHOD 2 Help prompt Figure 2 3 Main Screen NOTE You can check the information on the POWER UP screen
103. nnector labeled VAC PUMP near the right rear corner of the card Make sure the cable is fully seated in the connector Push the mechanical chassis drawer back in place being careful not to pinch any of the cables Tighten the drawer lock Turn on the power Go to the DEGAS PUMP CALIBRATION AND STATUS screen and retry the calibration see Section C 3 3 If the message appear again the degas assembly may need to be replaced Contact Dionex Technical Support for assistance Doc 031612 03 10 03 4 11 GS50 Gradient Pump 4 8 4 9 4 12 Vacuum Degas Assembly Low Vacuum The GS50 monitors the vacuum degas reading at 1 minute intervals If the vacuum degas is less than the monitoring value the degas pump turns on If the vacuum reading is 2000 counts or more less than the monitoring value when the degas pump turns off the following message appears LOW VACUUM ALARM Check DEGAS OPTIONS settings or refer to service manual Go to the DEGAS OPTIONS screen see Section C 1 6 Increase the CYCLE DURATION time and or decrease the TIME BETWEEN CYCLES If this does not resolve the problem the vacuum degas assembly may need to be replaced Contact Dionex Technical Support for assistance Relays or TTLs Inoperative Incorrectly installed cables Make sure the cables between the appropriate relay function and the input or output unit are connected to the correct slots see Appendix D e TTL input related error 1 The c
104. nt profile for this method Eluent B Eluent 50 Eluent A 15 Time min Figure 3 10 Gradient Curve Profile Doc 031612 03 10 03 3 19 GS50 Gradient Pump 3 20 Go to the METHOD screen and enter a method number 3 for example in the EDIT field e If Method 3 currently exists and you want to retain it enter a new unused method number in the EDIT field e If Method 3 currently exists and you want to delete it move the cursor to TIME INIT and press Delete twice Move to the FLOW field of the INIT step and enter a flow rate of 1 50 Move the cursor to the A field of the TIME 0 step and press Enter 100 of eluent A is automatically filled in Move the cursor down to the next line and enter 3 in the TIME field Move to the A field and press Enter 100 of eluent A is automatically filled in Move the cursor to the C field and enter 5 to run gradient curve 5 Figure 3 11 illustrates the METHOD screen as it appears so far METHOD EDIT 3 SAVE TO 3 LIMITS 0 5000 PSI TIME A B C D C V FLOW INIT 100 0 ELE 1 50 55 0 00 100 0 7 3 00 100 0 C Help prompt Figure 3 11 Curved Gradient Method Example After Step 5 6 Move the cursor down to the next line and enter 13 in the TIME field Move the cursor to A and enter 0 Move the cursor to B and enter 100 Move the cursor to the C field and enter 2 to run gradient curve 2 This marks the end of the isocratic section of the run and the
105. ntive maintenance requirements Lists possible causes of problems as well as step by step procedures to resolve them Contains step by step instructions for routine service and parts replacement procedures Lists the GS50 specifications and installation site requirements Describes how to install the GS50 Illustrates and describes all of the screens that can be displayed on the GS50 front panel Describes Relay and TTL input and output functions provides setup examples Lists spare parts for the pump GS50 Gradient Pump 1 2 1 1 2 2 gt DANGER gt WARNING gt CAUTION IMPORTANT Typefaces e Capitalized bold type indicates a front panel button Press Enter to begin running the method e Uppercase bold type indicates the name of a menu or screen or an on screen entry Go to the METHOD screen Move the cursor to the EDIT field Safety Messages and Notes This manual contains warnings and precautionary statements that can prevent personal injury and or damage to the GS50 when properly followed Safety messages appear in bold type and are accompanied by icons as shown below Indicates an imminently hazardous situation which if not avoided will result in death or serious injury Indicates a potentially hazardous situation which if not avoided may result in death or serious injury Indicates a potentially hazardous situation which if not avoided may result in minor or moderate inju
106. o the INITial conditions or enter an elapsed time of less than 15 10 for example in the MIN field 3 22 Doc 031612 03 10 03 3 Operation and Maintenance 3 5 Routine Maintenance This section describes routine maintenance procedures that the user can perform All other maintenance procedures must be performed by qualified Dionex personnel As Needed e Refill the eluent reservoir e Make fresh eluent e Refill the bottle of flush solution for the piston seal wash assembly Daily e If you are using a combination of eluents that contains both salt or base and solvent wash the pump pistons frequently The GS50 piston seal wash assembly continuously rinses the back of the piston seals to remove salt crystals and prolong the life of the seals To use this feature an external water source must be connected see Section B 2 6 for more information Also do not store the pump with salt buffers in the flow path Flush the entire flow path excluding the column with deionized water to prevent salt crystals from causing clogs e Check the entire mechanical chassis for leaks from the eluent proportioning valve assembly valve connections eluent reservoirs and vacuum degas chamber if the vacuum degas assembly is installed Tighten or replace any leaking fittings Wipe up liquid spills and rinse dried reagents off pump components with deionized water e Clean eluent reservoirs thoroughly inside and out with deionized water and l
107. of an existing method if you plan to modify this method and save the changes to a new method number After entering an unused method number the screen resembles the example in Figure 3 5 METHODEDIT 5 SAVE TO 5 LIMITS 0 5000 PSI TIME A B C D C V FLOW INIT 100 0 L 2 00 gt 0 00 i gt gt gt Help prompt Figure 3 5 Method Screen Creating a New Method 5 Inthe LIMITS field set the low and high pressure limits see Section 3 1 5 6 Each method begins with two timed steps see Figure 3 5 an initial conditions step containing INIT in the TIME column and a time zero step containing 0 00 in the TIME column Enter parameters for both steps 7 Enter a new step using one of these methods e Move the cursor to the TIME field below the last step in the method and enter the elapsed time at which to start the new step Press Enter or a cursor arrow button e Move the cursor to any TIME field on the screen and press Insert to add a new step after the cursor position Enter the elapsed time at which to start the new step When you press Enter or a cursor arrow button the GS50 Moduleware moves the new step to the correct chronological position Doc 031612 03 10 03 3 11 GS50 Gradient Pump 8 Continue entering parameters for the new step When you finish move the cursor to the SAVE TO field and do one of the following e After editing an unused method press Enter e After editing an existing method
108. ompatible with the output signals provided by Dionex modules If the device connected to the GS50 outputs a different signal type select the appropriate mode from the TIME FUNCTION IN screen see Figure D 3 TIME FUNCTION IN MODE INVERTED EDGE PUMP MOTOR OFF ON TTL1 HOLD RESET INIT COND RUN TTL2 Help prompt Figure D 3 Time Function In Screen There are four input signal modes e Normal Edge In normal edge operation the negative falling edge of a signal turns on the function and the positive rising edge turns off the function see Figure D 4 For example a negative edge sent to TTL turns on the pump motor and a positive edge turns off the motor e Inverted Edge The inverted edge mode operates identically to the normal edge mode except that the positive and negative edges are reversed in function Doc 031612 03 10 03 D 3 GS50 Gradient Pump e Normal Pulse In normal pulse operation the negative falling edge of the TTL signal is the active edge and the positive rising edge is ignored For example applying a negative pulse to TTL1 when the pump motor is off turns on the motor The minimum pulse width guaranteed to be detected 1s 50 ms The maximum pulse width guaranteed to be ignored as noise or invalid is 4 ms The action of the GS50 is undefined for pulses less than 50 ms or greater than 4 ms e Inverted Pulse The inverted pulse mode operates identically to the normal pulse mode except that the posit
109. on the keypad that corresponds to the screen number For example press 3 to view the METHOD screen e You can edit the reverse video fields on a screen Other fields display information but cannot be edited e To edit a parameter use the four directional arrow buttons to position the cursor in the reverse video field Use the numeric buttons to enter a variable value Use the Select A and Select V buttons to choose an entry from among predetermined options 2 2 Doc 031612 03 10 03 Button Off On Prime 1 Insert 1 Doc 031612 03 10 03 2 Description Press Enter to execute the selected value or press an arrow button to simultaneously confirm the selected value and move the cursor to the next field When you press a front panel button a high pitched beep sounds If an error occurs this beep is lower in frequency You can disable the beeps from the MODULE SET UP screen see Section C 1 7 Function Turns the pump motor off and on In Direct control see Section 2 8 turning on the motor causes it to pump isocratically using the displayed eluent percentages and flow rate In Method control see Section 2 8 2 turning on the motor causes it to pump at the eluent percentages and flow rate for the elapsed time of the selected method or when the method clock is at INIT at the initial conditions Operates the pump in the mode reserved for priming the pump heads If the pump motor is off when Prime is press
110. ops and goes to Hold The front panel displays the current status of the program that was running when the pressure limit was tripped To select the pressure limits from the front panel 1 Go to the DETAIL or METHOD screen and move the cursor to the LIMITS field see Figure 3 1 DETAIL SCREEN RLY1 0 A 2125 PSI RLY2 1 B 2 00 mL MIN TTL1 0 C INJECT SAMPLE TTL2 1 A D COLUMN LIMITS 1200 1900 PSI DIRECT CNTRL Help prompt Figure 3 1 Detail Screen Setting Pressure Limits 3 4 Doc 031612 03 10 03 3 Operation and Maintenance 2 Enter a low pressure limit between 1 4 and 2 8 MPa 200 and 400 ps The setting depends on the system operating pressure NOTE To prevent the low pressure limit from being tripped each time the GS50 power is turned on the limit is inactive for the first several piston strokes If a break occurs after this period the low pressure limit is activated instantaneously 3 Enter a high pressure limit that is 3 4 MPa 500 psi higher than the low pressure limit and 2 8 to 3 4 MPa 400 to 500 psi above the normal system operating pressure NOTE The GS50 is equipped with a pressure alarm limit that prevents pump operation above 35 MPa 5076 psi 3 1 6 Calibrating the Pressure Ripple During GS50 operation a slight momentary change in pressure known as a pressure ripple occurs each time one piston moves forward as the other piston moves backward This pressure ripple during the transitio
111. or shuts off the SRS power The 5 minute delay allows momentary flow interruptions without disturbing the SRS Follow the procedure below to implement automatic SRS power control 1 Go to the GS50 PUMP OPTIONS screen and set TTL2 OUTPUT USAGE to 0 FLOW see Figure B 5 PUMP OPTIONS TTL2 OUTPUT USAGE 0 FLOW PRESSURE UNITS PSI COMPRESSIBILITY xxxx DEGAS PUMP OPTION YES Help prompt Figure B 5 Pump Options Screen 2 Go to the detector TTL FUNCTION IN screen and assign TTL3 to SRS OFF ON 3 Inthe GS50 Ship Kit P N 061222 locate one pair of twisted black and red wires P N 043598 and two green TTL connector plugs P N 921019 4 Insert the twisted wires into the plugs The signal wire red goes on the top of each plug and the ground wire black goes on the bottom of each plug 5 Plug one end of the cable into TTL2 OUT on the GS50 The TTL connectors are located behind the upper door Route the cable through the upper chase of the GS50 and into the upper chase of the detector see Figure B 6 Plug the other end into TTL3 IN on the detector be sure to maintain the wire orientation described in Step 4 Doc 031612 03 10 03 B 15 GS50 Gradient Pump Detector TTL3 IN SRS OFF ON TTL Plug P N 921019 Detector Chase Wires P N 043598 Route through detector and pump upper chases TTL2 OUT 0 FLOW TTL Plug P N 921019 Pump Upper Chase Figure B 6 TTL Connections for SRS Power Control De
112. ote mode 2 19 2 21 Clock See Method clock Column switching valve Selecting C 8 Columns Backpressure 4 5 Damage from IPA B 14 Compressibility factor eluents C 12 Concave linear curve 2 23 Connect command 2 21 Connectors LC AIR 2 10 LC COMM 2 10 PRES 2 11 Rear panel 2 18 Connectors on electronics cards 2 9 TTL Relay control 2 11 Control modes 2 21 Direct 2 20 Method 2 20 2 21 Selecting 2 20 Control panel 2 1 Keypad 2 2 Conventions in manual 1 4 Convex gradient curves 2 23 2 24 Index 1 GS50 Gradient Pump CPU LAN card 2 11 4 6 B 6 LED colors 4 4 Creating a method 3 10 Cursor movement 2 2 2 5 Curve field Detail screen C 5 Curved gradient Method example 3 19 Curves See Gradient curves Types 2 23 D Daily maintenance 3 23 Danger icon 1 4 1 5 Definition 2 2 Degas assembly 2 16 Calibration fails 4 11 Calibration procedure C 22 Daily maintenance 3 23 Vacuum is low 4 12 Degas Options screen C 9 Degas pump 4 1 Calibration C 22 Frequency of operation 4 2 Specifications A 3 Degas Pump Calibration and Status screen C 22 Degassing eluents 1 1 2 16 3 1 Delay volume 1 2 A 2 Delay volume conversion kit 1 2 Delete Method 2 4 3 14 Method steps 2 4 Delete button 2 4 Detail screen C 5 Diagnostic Menu C 14 Calibration Menu C 20 Degas Pump Calibration and Status C 22 Diagnostic Tests C 18 Flow Calibration C 23 Leak Sensor Calibration and Status
113. our different eluent components each eluent can be proportioned from 0 to 100 Dual piston in series variable speed 100 uL per revolution 0 0 mL min or from 0 05 to 5 0 mL min in increments of 0 01 mL min Note The GS50 is optimized for operation at 1 5 mL min or less Low pressure 0 to 100 in 0 1 increments Four concave four convex one linear lt 1 of set value or 2 uL min whichever is greater lt 0 2 lt 1 35 MPa 5000 psi maximum 691 kPa to 35 MPa 100 to 5000 psi in increments of 0 1 MPa 14 psi trips instantaneously 0 to 34 MPa 0 to 4900 psi in increments of 0 1 MPa 14 psi a start up delay prevents false tripping Low delay volume lt 400 uL Standard delay volume lt 800 uL lt 1 at 13 8 MPa 2000 psi and 1 0 mL min Doc 031612 03 10 03 A Specifications A 6 Control Modes Remote Limited remote operation via TTL input logic level and TTL output and Relay contact closures or full remote programming and control via the Dionex DX LAN interface Local Front Panel Methods Stores up to 100 separate methods 00 through 99 the actual number depends on available memory Each method can contain up to 50 separate steps Storage Nonvolatile memory protects against the loss of programs when the pump is turned off or if there is a power failure A 7 Vacuum Degas Assembly Optional Channels 4 channel degas chamber with degas membranes Pump Dual stage diaphragm vacuum pump
114. piston seal allows leakage past the piston as well as leakage from the seal wash housing The pump may be difficult to prime flow rates may be unstable and there may be baseline noise Removing the Head and Piston li Turn off the main power switch to ensure that you do not unintentionally start the GS50 2 Disconnect all tubing connections to the pump head 3 Remove the two acorn nuts and washers from the pump head see Figure 5 1 4 Carefully disengage the head from the piston by pulling the head straight off and away from the studs Lateral motion while disengaging the pump head from the piston may break the piston CAUTION Un mouvement lat ral pendant la s paration de la t te et du piston peut casser le piston MISE EN GARDE Vermeiden Sie Seitwartsbewegungen wenn Sie den Kopf vom Kolben l sen Andernfalls kann der Kolben brechen VORSICHT 5 Place the head front end down on a clean work surface and lift off the spacer to expose the piston seal see Figure 5 2 or Figure 5 3 6 The piston does not come off as part of the pump head assembly because it is captured by a magnetic retention system After removing the pump head hold the shaft of the piston near the base tilt the piston slightly and pull the piston away from the pump Doc 031612 03 10 03 Outlet Check Valve Assembly P N 047657 Piston Seal P N 055870 Spacer with Drain _ re PIN 057913 Piston Rinse Seal PIN 048722 N
115. play panel backlight to BACKLIGHT low medium or high KEY ACTUATION Enables and disables the beep that occurs when a SOUND front panel key is pressed ENTRY ERROR Enables and disables the beep that occurs when a SOUND wrong entry is made HELP LANGUAGE Selects the language for Help and error messages English or Japanese Doc 031612 03 10 03 C 11 GS50 Gradient Pump C 1 8 Pump Options Use the PUMP OPTIONS screen to select default operating parameters for the GS50 PUMP OPTIONS TTL2 OUTPUT USAGE NORMAL PRESSURE UNITS PSI COMPRESSIBILITY xxxx DEGAS PUMP OPTION YES Help prompt Figure C 9 Pump Options Screen TTL2 OUTPUT USAGE PRESSURE UNITS COMPRESS IBILITY DEGAS PUMP OPTION Specifies the active state of the TTL2 output signal NORMAL defines TTL2 as a regular TTL output programmed in a method 0 FLOW can be used to automatically switch off the power to the Self Regenerating Suppressor SRS when the pump flow stops For instructions on how to set up this function see Section B 3 Sets the unit of measure for pressure psi MPa or bar Sets the eluent compressibility factor a feature that helps to optimize the solvent flow stability The settable range is 0 to 2500 counts the default 460 counts is optimized for deionized water Indicates whether the optional vacuum degas assembly is installed see Section 2 5 Doc 031612 03 10 03 C User Interface C 1 9 Time Function In
116. ponents DIAGNOSTIC TESTS POWER LOOP COM PUMP LEAK CPU DSP COM OVEN COM Help prompt Figure C 15 Diagnostic Tests Screen When the GS50 power is turned on some of the electronics tests run automatically To run a test manually position the cursor in the edit field next to the test name press a Select button to select the asterisk and press Enter The table below explains the test status indicators Character Test Status Test did not run gt Test is in progress Test passed re Wo Test failed POWER Checks that the DC power supplies are within 5 tolerance CPU Checks the CPU internal configuration and Moduleware checksum LOOP COM Checks the serial communication hardware using a loop back cable Doc 031612 03 10 03 DSP COM PUMP LEAK OVEN COM EXTERNAL LEAK Doc 031612 03 10 03 C User Interface Checks communication between the pump CPU and the DSP digital signal processor hardware Checks the GS50 leak sensor hardware for a correct open circuit or short circuit condition Checks communication between the GS50 and the LC30 Chromatography Oven Checks the leak sensor hardware in the LC10 Chromatography Organizer or LC25 Chromatography Oven GS50 Gradient Pump C 3 Calibration Screens C 3 1 C 20 Calibration Menu The CALIBRATION MENU lists the calibration screens To display the menu select option 3 on the DIAGNOSTIC MENU CALIBRATION MENU 1 LEAK
117. put is inoperative 4 13 TTL input operation Functions controlled D 3 Method clock control D 3 TTL Relay control 1 1 2 19 2 21 D 1 Connectors 2 11 Does not operate 4 12 Example connections D 6 Input operation D 3 Input signal modes D 3 Installation D 5 Output operation D 2 Setting outputs C 5 C 8 TTL2 output is inoperative 4 13 TTL2 output usage B 15 C 12 U Usage Log screen C 17 User interface See Display screens V VAC PUMP connector 4 11 Vacuum degas assembly 1 1 2 16 Components 2 16 Daily maintenance 3 23 Daily shutdown 3 25 Electrical connections 4 11 Priming B 12 B 13 See also Degas pump 1 1 Specifications A 3 Valves Check valve service procedures 5 1 See also Valve type Doc 031612 03 10 03 Waste valve 2 13 Waste valve location 2 13 Voltage selection B 3 W Warning icon 1 4 1 5 Warranty voiding 5 1 Waste lines B 7 Waste valve 2 13 Leakage from knob 4 8 Opening 2 13 O ring replacement procedure 5 11 Waste line B 7 Weekly maintenance 3 24 Y Yearly maintenance 3 24 Doc 031612 03 10 03 Index Index 9 GS50 Gradient Pump Index 10 Doc 031612 03 10 03
118. radient Pump CAUTION MISE EN GARDE VORSICHT Observe the warning label on the inside of the lower door The arrows on the label indicate moving mechanical parts that present pinch hazards when the pump is on and the mechanical chassis drawer is open Do not operate the pump when the chassis drawer is pulled out Respectez l tiquette d avertissement appos e a l int rieur de la porte inf rieure Les fl ches sur l tiquette indiquent des pieces m caniques mobiles qui posent un danger de pincement lorsque le GS50 est sous tension et le tiroir m canique est ouvert N utilisez jamais le GS50 avec le tiroir du ch ssis m canique ouvert Beachten Sie den Warnhinweis auf der Innenseite der unteren T r Die Pfeile auf dem Aufkleber weisen auf bewegliche mechanische Teile hin Bei eingeschalteter Pumpe und geoffneter Mechanik besteht hier die Gefahr von Quetschungen Die Pumpe darf nicht mit herausgezogenem Mechanikeinschub betrieben werden 2 4 Interior Components 2 4 1 2 12 Pump Heads Primary Pump Head The primary head pumps the selected mobile phase into the secondary head see Figure 2 6 The inlet and outlet check valves are located on the bottom and top respectively of the primary pump head The priming valve is on the front of the pump head To open the priming valve turn the knob one quarter to one half turn counterclockwise When the priming valve is opened liquid can flow into and out of the primary pump hea
119. ress Off On to have the pump start delivering the eluent mixture 5 Ifthe method clock is in hold press Hold Run to begin running the method 3 4 2 Linear Gradient Method Example Here is a summary of the linear gradient method steps e Create Method 2 to begin under isocratic conditions with 100 eluent A at 1 0 mL min e Hold this for 5 minutes TIME 5 00 e Begin a 10 minute segment in which the amount of eluent B is increased and the amount of eluent A is decreased until the mixture is 20 eluent A and 80 eluent B TIME 15 00 e Hold this for 5 minutes TIME 20 00 e At 20 10 minutes return to 100 eluent A and re equilibrate the system for the next analysis Figure 3 7 illustrates the gradient profile for this method 3 16 Doc 031612 03 10 03 Eluent Doc 031612 03 10 03 3 Operation and Maintenance Eluent B Eluent A Time min Figure 3 7 Linear Gradient Method Profile Go to the METHOD screen and enter a method number 2 for example in the EDIT field e If Method 2 currently exists and you want to retain it enter a new unused method number in the EDIT field e If Method 2 currently exists and you want to delete it move the cursor to TIME z INIT and press Delete twice Move to the FLOW field of the INIT step and enter a flow rate of 1 00 Move the cursor to the A field of the TIME 0 step and press Enter 100 of eluent A is automatically filled in Move th
120. roniques de la pompe Aucun des composants sur les cartes ne peut tre r par par l utilisateur Toute r paration doit tre effectu e par un personnel qualifi utilisant des een proc dures correctes de d charge lectrostatique Halten Sie sich von der Elektronik des GS50 fern Die Elektronik kann nicht vom Anwender gewartet werden Falls ein Service erforderlich ist ist dieser von qualifiziertem Personal durchzuf hren Dabei VORSICHT m ssen die geeigneten Verfahren zur elektrostatischen Entladung ESD eingehalten werden 4 6 Doc 031612 03 10 03 4 Troubleshooting e The following error message is displayed Encoder Index not found l Turn off the GS50 by pressing the main power switch 2 Verify that the cables connected to the DSP card in slot 1 of the GS50 electronics chassis are fully seated see Figure 2 5 3 Turn on the GS50 again If the encoder index error message reappears contact Dionex for assistance e Electrical cables improperly installed 1 Place the pump in LOCAL mode DIRECT CONTROL see Section 2 8 Press Off On to start the pump 2 Ifanon zero flow rate is displayed and the keypad LED is on verify that the electrical cables in the mechanical chassis are properly installed Turn off the GS50 power by pressing the power switch b Using a 7 mm open end wrench or your fingers loosen the lock on the mechanical chassis drawer The lock is on the lower right side of the chassis between
121. ry Indicates that the function or process of the instrument may be impaired Operation does not constitute a hazard Doc 031612 03 10 03 gt DANGER gt AVERTISSEMENT gt MISE EN GARDE gt ACHTUNG gt WARNUNG gt VORSICHT 1 Introduction Messages d avertissement en frangais Signale une situation de danger imm diat qui si elle n est pas vit e entrainera des blessures graves a mortelles Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entrainer des blessures graves a mortelles Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entrainer des blessures mineures mod r es Egalement utilis pour signaler une situation ou une pratique qui pourrait gravement endommager l instrument mais qui n entrainera pas de blessures Warnhinweise in Deutsch Bedeutet unmittelbare Gefahr MiRachtung kann zum Tod oder schwerwiegenden Verletzungen f hren Bedeutet eine m gliche Gef hrdung Mi achtung kann zum Tod oder schwerwiegenden Verletzungen f hren Bedeutet eine m gliche Gef hrdung Mi achtung kann zu kleineren oder mittelschweren Verletzungen f hren Wird auch verwendet wenn eine Situation zu schweren Sch den am Ger t f hren kann jedoch keine Verletzungsgefahr besteht Informational messages also appear throughout this manual These are labeled NOTE and are in bold type NOTE NOTES call attention to
122. s Delete twice 3 3 5 Changing the Running Method To change from the method currently running to a different method go to the MAIN or DETAIL screen enter the new method number in the METHOD field and press Enter The new method begins running using the parameters specified in the step for the current elapsed time If you prefer to start the method at the INITial conditions press Reset 3 3 6 Controlling the Method Clock The method clock is controlled by the Hold Run button the Reset button and the MIN fields on the MAIN and DETAIL screens e To start and stop the method clock press Hold Run e To reset the method clock to INIT press Reset e To set the clock to a specific elapsed time either enter the time in the MIN field on the MAIN screen or press a Select button to step through the programmed step times when the appropriate step time 1s displayed press Enter The method will start or continue running using the method parameters specified for that time 3 14 Doc 031612 03 10 03 3 4 3 Operation and Maintenance Example Methods The examples in this section provide step by step instructions for creating three types of methods isocratic linear gradient and curved gradient The last example demonstrates how to edit a running method Before entering any ofthe example methods set the pump to Local mode Method control Go to the MAIN or DETAIL screen and if necessary toggle from REMOTE to LOCAL and fro
123. se seal is made of soft plastic Do not press on the seal with anything hard or sharp including your fingernail If the seal is nicked or gouged it will not seal properly and may result in leaks Place the new O ring P N 059283 into the groove in the guide Remove the O ring from the groove in the flat side of the spacer and replace it with the new O ring P N 014895 In one hand hold the guide with the O ring and piston rinse seal facing up this prevents the O ring from falling out In the other hand hold the spacer with the cavity facing down Gently press the guide into the cavity in the spacer until it is fully seated Doc 031612 03 10 03 5 Service Removing the Piston Seal from the Head M 3 Fill the head cavity with deionized water by injecting the liquid through either the piston opening or the inlet check valve Reinsert the piston approximately 1 8 inch into the seal If this is the primary pump head install a 10 32 fitting plug P N 042772 on the outlet check valve Tighten the plug If this is the secondary pump head install a 10 32 fitting plug P N 042772 in both the inlet and outlet ports Tighten the plugs Push the piston into the head This should hydraulically unseat the seal from the head Remove the piston and pull off the seal NOTE If the piston seal is not removed make sure the 10 32 fitting plug s are tight and add more water Make sure the head contains no air bubbles an
124. see Figure B 4 by turning the knob one quarter to one half turn counterclockwise Press Off On to turn on the pump motor Open the priming valve on the primary pump head see Figure B 4 by turning it one quarter to one half turn counterclockwise Use the syringe to slowly push alcohol through the pump If the operating backpressure is high this requires more force NOTE Be careful not to push any air trapped in the syringe through the pump Check the waste line from the secondary pump head to verify that there are no air bubbles and that alcohol is traveling down the waste line Close the priming valve Do not overtighten Disconnect the syringe from the priming valve Let the pump run for several minutes to purge alcohol from the pump heads and then press Prime and flush the heads with the desired eluent Isopropyl alcohol may damage some columns Be sure to thoroughly rinse the alcohol from the pump before going on to Step 8 10 Close the waste valve Do not overtighten Select the flow rate required for the application Press Off On to turn off the pump motor The pump is now ready for operation Doc 031612 03 10 03 B Installation B 3 Automatic SRS Power Control Optional TTL connections can be used to automatically switch off the power to a Self Regenerating Suppressor SRS when the pump flow stops In this setup the GS50 sends a TTL signal to the detector and then after a 5 minute delay the detect
125. strip of eight 2 pin connectors on the GS50 electronics chassis provides two relay outputs two TTL outputs and four TTL inputs see Figure D 1 NOTE Before connecting the GS50 to a non Dionex device check the user s manual for the device to verify that it is compatible with the GS50 TTL and relay signals e If necessary connect the outputs to the TTL or relay inputs of a device to trigger functions in the connected device e Ifnecessary connect the inputs to a device to trigger two pump functions pump motor on off and method clock on off For a description of TTL and relay output operation or TTL input operation respectively see Section D 1 or Section D 2 For instructions on how to connect the GS50 TTLs and relays to other devices see Section D 3 RLY 1 OUTPUT 3 RLy 2 OUTPUT Main Power fr TTL 1 output Switch Jd TTL 2 output 2 TTL 1 INPUT TTL 2 input s TTL 3 INPUT Unused w 5 TTL 4inPuT Unused The terminal strip is located behind the upper front door Figure D 1 TTL Relay Connector Strip GS50 front view without upper door Doc 031612 03 10 03 D 1 GS50 Gradient Pump D 1 TTL and Relay Output Operation The GS50 provides two TTL outputs and two relay contacts for the control of functions in external devices For example the external device might be an integrator autosampler or another Dionex module After connecting the TTL and Relay outputs see Sec
126. te Men 2x13 35425 55a MESE OERS SENS C 14 2 2 PoOwerUp Seteen uoa SEES een C 15 2 9 ProSus SA ieS raarste bok IE HERE Red ken C 16 Cl WALE LOP TT C 17 25 DiasnosucTes uox dac ie pet RE owed Weeks C 18 Doc 031612 03 10 03 V GS50 Gradient Pump vi C 3 Calibration Screens s usd oe EET C 3 1 Calibration Menu C 3 2 Leak Sensor Calibration and Status C 3 3 Degas Pump Calibration and Status C 3 4 Flow Calibration C 3 5 Pressure Offset Calibration C 3 6 Pressure Ripple Calibration D TTL and Relay Control D 1 TTL and Relay Output Operation D 2 TTL Input Operation D 2 TTL Input Signal Modes D 3 TTL and Relay Connections D 3 1 Example Connections E Reordering Information Doc 031612 03 10 03 1 Introduction 1 1 Overview The GS50 Gradient Pump is a microprocessor based eluent or mobile phase delivery system Its variable speed serial piston design ensures pulse free pumping for the most demanding applications The GS50 delivers mixtures of up to four mobile phase components at precisely controlled flow rates The selected mobile phase composition can be delivered as isocratic isocratic proportioned linear ramp step curved or any combination of these A DSP Digital Signal Processor provides high speed extremely accurate control of pump flow The two basic modes of pump control Direct control and Method control enable
127. tector and pump front views without front doors B 16 Doc 031612 03 10 03 C User Interface This appendix describes and illustrates all of the screens that can be displayed on the GS50 front panel There are three types of screens operational diagnostic and calibration see Figure C 1 e Operational screens allow the user to create edit and run methods that control pump operation as well as select default parameters e Diagnostic screens allow the user to access diagnostic information and tests e Calibration screens allow the user to calibrate pump functions flow rate pressure offset etc MENU of SCREENS Operational Screens 1 2 3 4 5 6 7 MAIN DETAIL METHOD DEGAS MODULE PUMP TIME DIAGNOSTIC SCREEN SCREEN OPTIONS SETUP OPTIONS FUNCTION IN MENU METHOD EVENTS Diagnostic Screens PRESSURE PRESSURE OFFSET RIPPLE CALIBRATION CALIBRATION Figure C 1 GS50 Menu Structure Doc 031612 03 10 03 C 1 GS50 Gradient Pump C 1 Operational Screens C 1 1 C 2 Menu of Screens The MENU of SCREENS provides top level access to the menu structure MENU of SCREENS 1 MAIN SCREEN gt MODULE SETUP 2 DETAIL SCREEN 6 PUMP OPTIONS 3 METHOD 7 TIME FUNCTION IN 4 DEGAS OPTIONS 8 DIAGNOSTIC MENU Help prompt Figure C 2 Menu of Screens NOTE You cannot select the DEGAS OPTIONS screen unless the vacuum degas assembly see Section 2 5 is installed There are two ways to view a screen option e
128. ter to one half turn counterclockwise PRESSURE The current pressure reading from the transducer OFFSET The calibrated offset value C 24 Doc 031612 03 10 03 C User Interface C 3 6 Pressure Ripple Calibration Use the PRESSURE RIPPLE CALIBRATION screen to calibrate the pressure ripple For more details see Section 3 1 6 PRESSURE RIPPLE CALIBRATION CALIBRATION DURATION Help prompt READY xxx xx min Figure C 21 Pressure Ripple Calibration Screen CALIBRATION DURATION Doc 031612 03 10 03 CALIBRATE To start a pressure ripple calibration sequence toggle the field from READY to CALIBRATE and press Enter During calibration the pump runs at the currently selected flow rate or at 1 0 mL min if the selected flow rate is zero Notes a The Menu button is disabled while the calibration sequence is running b To terminate a calibration sequence toggle the field to READY and press Enter GET DATA Updates the pressure ripple data with the latest operating ripple data DEFAULTS Sets the pressure ripple data to the factory default values these are the theoretical values for pump operation at 1 0 mL min with 15 MPa 2200 psi of backpressure Reports the time remaining until the calibration sequence is finished At a flow rate of 1 0 mL min it takes about 13 minutes to run the calibration sequence C 25 GS50 Gradient Pump C 26 Doc 031612 03 10 03 D TTL and Relay Control The
129. the pump or changing the flow rate wait at least 5 minutes longer for flow rates below 1 0 mL min before starting an analysis This delay allows the digital signal processor DSP program to Stabilize the flow rate 3 3 Running Under Method Front Panel Control Method control is used primarily when the GS50 must communicate with non Dionex software or when the pump is under integrator control This section presents an overview of how to create edit and run methods from the GS50 front panel for step by step examples see Section 3 4 When entering parameters on the METHOD screen observe these guidelines e After starting the pump or changing the flow rate wait at least 5 minutes longer for flow rates below 1 0 mL min before beginning an analysis The delay allows the digital signal processor DSP program to stabilize the flow rate e Inthe A B C and D columns enter a decimal percentage value from 0 1 through 100 for the eluent composition The pump cannot run unless the combined total of all four eluents equals 100 e In the V column select the injection valve position L for load or I for inject e Inthe FLOW column enter a flow rate from 0 5 to 5 0 mL min The flow rate is adjustable in increments of 0 01 mL min NOTE The GS50 is optimized for operation at 1 5 mL min or less Doc 031612 03 10 03 3 9 GS50 Gradient Pump 3 3 1 3 10 To have the pump deliver a linear or curved gradient see Section 2
130. tion D 3 toggle the output states on and off from either the DETAIL screen see Section C 1 3 or the METHOD events screen see Section C 1 5 e To turn on a TTL or relay output set the corresponding output field on the DETAIL screen or METHOD events screen to closed e To turn off a TTL or relay output set the corresponding output field on the DETAIL screen or METHOD events screen to 0 open For example when TTL2 is connected to the Load relay on the Dionex AS40 Autosampler setting TTL2 to 1 as shown in Figure D 2 sends a signal to the AS40 to start the load cycle DETAIL SCREEN RLY1 0 A 2125 PSI RLY2 1 B 2 00 mL MIN TTL1 O 7o C INJECT SAMPLE TTL2 1 A 0 D COLUMN CURVE 5 LIMITS 0 5000 PSI REMOTE METHOD 5 10 25 MIN Help prompt Figure D 2 Detail Screen D 2 Doc 031612 03 10 03 D TTL and Relay Control D 2 TTL Input Operation The two TTL inputs can be connected to devices capable of providing TTL output signals The signal from the connected devices can trigger the following functions in the GS50 e TTL input 1 turns the pump motor on and off e TTL input 2 turns the pump method clock on Run and off Hold Reset Hold Reset resets the method clock to zero and executes the INIT conditions although the method cannot run unless the pump motor is on D 2 1 TTL Input Signal Modes The GS50 TTL inputs respond to four different types of device output signals The default signal mode normal edge is c
131. uppressor SRS To use TTL2 for another function set the TTL2 OUTPUT USAGE field to NORMAL see Section C 1 8 4 11 Poor Chromatographic Reproducibility Liquid leaks 1 Check for leaks from the piston seals Replace the piston seal on any head with a leak see Section 5 2 2 Check for leaks throughout the remainder of the system including the check valves injection valve and columns Tighten or replace fittings as needed Pump not primed Prime the pump see Section B 2 7 Liquid lines incompletely flushed after an eluent change Before beginning operation attach a syringe to the priming valve see Figure B 4 and draw at least 2 5 mL of the new eluent 20 mL if the vacuum degas assembly is installed through the liquid lines Malfunctioning proportioning valve Replace the proportioning valve assembly see Section 5 5 Doc 031612 03 10 03 4 13 GS50 Gradient Pump e Malfunctioning check valves Clean and or replace the check valves see Section 5 1 Insufficient mixing 1 Some mobile phases are difficult to mix If you notice shifting retention times or an unstable baseline install an additional mixer see Section 2 4 3 2 The gradient mixer may be dirty or contaminated Install a new mixer see Section 2 4 3 e Contaminated mobile phase 1 Clean all eluent reservoirs thoroughly inside and out with deionized water and let them air dry If a reservoir still seems dirty or if there is a slimy film on t
132. using 2 4 Resetting 2 4 Setting the elapsed time 3 14 Starting a method 3 12 Method control 2 20 2 21 3 9 Method events screen C 8 Method number C 4 Method screen C 6 Method steps Blank empty fields 3 10 Definition 2 22 Deleting 2 4 Entering 3 9 C 6 Inserting 3 11 Restoring the previous value 2 4 Setting step times C 6 Methods 2 2 Aborting 3 14 Control 3 9 Creating 3 10 Curved gradient example 3 19 Deleting 2 4 3 14 Deleting a step 3 13 Editing 3 13 Editing while running 3 15 3 21 Examples 3 15 Gradient curve types 2 23 Gradient eluent run 2 23 Gradient steps 2 23 INIT conditions 3 11 Inserting a step 2 3 Isocratic eluent run 2 23 3 15 Linear gradient example 3 16 Index 5 GS50 Gradient Pump Maximum number 2 22 Maximum number of steps 2 22 Running 3 12 Step gradient 2 24 Modes of operation See Operating modes Module Setup screen C 11 Moduleware 2 11 N Normal edge D 3 Normal pulse D 4 NOTES in manual 1 5 Numeric buttons 2 6 O Off On button 2 3 Operating modes Direct control 3 7 Local mode 2 19 2 21 Locked Remote mode 2 19 2 21 Method control 2 21 Selecting 2 19 TTL input 2 19 Operating pressure test 2 11 Operation 3 1 Aborting a running method 3 14 Creating a method 3 10 Curved gradient method example 3 19 Deleting a method 2 4 3 14 Editing a method 3 13 Editing a running method 3 21 Gradient curve types 2 23 Gradient eluent ru
133. ve causes erratic flow rates and pressures it may also cause the pump to lose prime and or be difficult to reprime l Turn off the main power switch to ensure that you do not unintentionally start the GS50 Disconnect the tube fittings from the inlet and outlet check valve housings on the primary pump head see Figure 5 1 Use a 1 2 inch wrench to loosen both check valve housings Remove the check valve housings and cartridges from the pump head Carefully remove the check valve cartridges from the housings Place the check valve housings and cartridges in a beaker with methanol Sonicate or agitate the parts for several minutes Rinse each check valve housing and cartridge thoroughly with filtered deionized water Doc 031612 03 10 03 5 1 GS50 Gradient Pump Outlet Check Valve h j Bi RI nam Spacer Primary kelk Pump Head DN To Injection Valve To Pressure Transducer Secondary Pump Head Proportioning Acorn Nut a K j alve 2 per pump head Waste Valve 1 F1 T 4 Pressure Transducer Beeren To Proportioning Valve From Proportioning Valve To Waste Figure 5 1 Pump Heads and Liquid Lines 6 Theinlet check valve assembly housing has a 1 4 28 port Replace the cartridge in the inlet check valve housing making sure the double hole end of the cartridge 1s visible
134. vex curves in eluent concentration over a specified time period The slope of the gradient is determined by the selected gradient curve see Figure 2 10 and the time between the starting and ending points of a gradient step Remember these key points e The curve number parameter determines whether the pump delivers a linear or curved gradient For an example of a linear gradient method see Section 3 4 2 For an example of a curved gradient method see Section 3 4 3 e Curve numbers are defined as follows Curve Number Gradient Type l 2 3 4 Convex 5 Linear power up default 6 7 8 9 Concave NOTE A curve number in a step instructs the pump to use the selected curve number when moving to that step from the previous step Because the INIT and TIME 0 0 steps are not preceded by any steps you cannot enter curve numbers for them e Curve 5 the default setting represents a linear gradient therefore the gradient slope does not change during a step if curve 5 1s selected Doc 031612 03 10 03 2 23 GS50 Gradient Pump e Convex curves cause rapid changes in eluent composition at the beginning of the curve and slower changes at the end Concave curves cause slower changes at the beginning of the curve and rapid changes at the end e Slope changes over time become more extreme as curves go from 6 to 9 more concave and from 4 to 1 more convex Figure 2 10 shows the eluent composition profiles corresponding to curves 1 throug
135. whenever you wish by selecting the screen from the DIAGNOSTIC MENU see Section C 2 1 The MAIN screen displays status information in enlarged characters to enable you to view the screen from a distance Use the MAIN screen to select the flow rate operating mode percentage of eluents to run and other operating parameters Doc 031612 03 10 03 2 7 GS50 Gradient Pump To access the remaining GS50 screens press the Menu button to display the MENU of SCREENS see Figure 2 4 MENU of SCREENS 1 MAIN SCREEN gt MODULE SETUP 2 DETAIL SCREEN 6 PUMP OPTIONS 3 METHOD 7 TIME FUNCTION IN 4 DEGAS OPTIONS 8 DIAGNOSTIC MENU Help prompt Figure 2 4 Menu of Screens There are two ways to view a screen option e Press the numeric button on the GS50 front panel keypad that corresponds to the screen number on the menu For example press 3 to select and display the METHOD screen e Move the cursor to the field containing the screen number and press Enter See Appendix C for a description of these screens 2 8 Doc 031612 03 10 03 2 2 2 Description Electronics Chassis The GS50 electronics chassis contains the power supply and several electronics cards printed circuit boards required for pump control see Figure 2 5 Connectors on the electronics cards enable the GS50 to communicate with other Dionex modules The electronics chassis is located behind the upper door of the GS50 enclosure To open the door pull on th

GS50 Gradient Pump Operator`s Manual

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