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1. 97 Appendix Biosafety Cabinet Dimensions 98 APPENDIX BIoSAFETY CABINET SPECIFICATIONS Electrical Data Cabinet Model Electrical Requirements 36210 10 to 09 115 VAC 60 Hz 1 Phase 12 Amps 36214 00 to 09 115 VAC 60 Hz 1 Phase 12 Amps Cabinet Model Electrical Requirements 36210 10 to 19 100 VAC 50 60 Hz 1 Phase 12 Amps 36214 10 to 19 100 VAC 50 60 Hz 1 Phase 12 Amps Cabinet Model Electrical Requirements 36210 20 to 29 230 VAC 50 60 Hz Phase 7 Amps Motor Specifications Cabinet Model Electrical Requirements 36210 00 to 09 36214 00 to 09 115 VAC 60 Hz 3 8 Full Load Amps 1 3 H P 1625 RPM Automatic Thermal Protection Cabinet Model Electrical Requirements 36210 10 to 19 115 VAC 50 60 Hz 3 9 Full Load Amps 1 3 H P 1625 RPM Automatic Thermal Protection 99 Appendix Biosafety Cabinet Specifications Cabinet Model Electrical Requirements 36214 10 to 19 115 VAC 50 60 Hz 6 Full Load Amps 1 2 H P 1625 RPM Automatic Thermal Protection Cabinet Model Electrical Requirements 36210 20 to 29 230 VAC 50 60 Hz 1 9 Full Load Amps 1 3 H P 1625 RPM Automatic T
2. All biohazard cabinets operate using the following principles e Filtration and retention of particulates by High Efficiency Particulate Air HEPA filter s e Laminar airflow sometimes e Directional airflow The major components in a Total Exhaust Biohazard Cabinet are The HEPA filter s The motor blower to force air through the unit A speed control for the motor Cabinet air intakes grilles ductwork and air balance controls e The remote blower 31 Chapter 4 Theory Of Operation And Safety Precautions HEPA Filters HEPA filters are disposable dry type particulate filters The filter material or media is typically made of borosilicate microfibers that are made into a thin sheet in a process similar to the production of paper This sheet is folded or pleated to increase its surface area The pleats are held in place by aluminum diffusers or by beads of glue that add rigidity to the media pack The media pack is then set into a suitable frame and the perimeter sealed to the filter frame as shown in Figure 4 1 A The HEPA filter media is very fragile Do not touch or contact the media e J surface If you think the surface of a HEPA filter is damaged DO NOT USE THE CABINET Have the HEPA filter integrity tested by a qualified certifier before using the cabinet HEPA Filters are only effective against particulate material Gases will pass through the filter Chapter 4 Theory O
3. User s Manual Purifier Delta Series Class II Type B2 Total Exhaust Biological Safety Cabinets Models 36210 Series 36214 Series Labconco s Mascot Labby the LABster Protecting your laboratory environment TA 2 LABCONCO For more information please contact us E mail sales expotechusa com Website www ExpotechUSA com Warranty Labconco provides a warranty on all parts and factory workmanship The warranty includes areas of defective material and workmanship provided such defect results from normal and proper use of the equipment The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco whichever is sooner except the following e Purifier amp Delta Series Biological Safety Cabinets carry a three year warranty from date of installation or four years from date of shipment from Labconco whichever is sooner e Carts carry a lifetime warranty Glassware is not warranted from breakage when dropped or mishandled This limited warranty covers parts and labor but not transportation and insurance charges In the event of a warranty claim contact Labconco Corporation or the dealer who sold you the product If the cause is determined to be a manufacturing fault the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation Under no circumstances shall Labconco Corporation be lia
4. CST Chapter 3 Getting Started 1 Before positioning the Purifier Base Stand in its final location decide the final height of the stand The working height can be anywhere between 30 to 36 inches 2 Select the height of the stand and slide four 4 leg extensions into base stand corner posts and attach with 2 inch long bolt flatwasher lockwasher and nut Ensure that the same height hole is selected for each leg Tighten the leg bolts securely See Figure 3 5 Figure 3 5 Remove and save these four nuts and bolts to secure the Purifier to the base stand Remove these four nuts and bolts to change the height of the base stand 3 Move the base stand into its final location Using a carpenter s level adjust each leveling foot until the stand is level in both planes as shown in Figure 3 6 You are now ready to lift the Purifier onto its stand 21 Chapter 3 Getting Started 22 Figure 3 6 Level the base stand DS SS Turn the four leveling feet to level the base Y stand to the floor 4 lt The Purifier is very top heavy Use caution when lifting or moving it 4 Carefully lift the Purifier onto the top rails of the base stand and slide it into position 5 Using drift punch or a medium sized screwdriver align one of the four
5. THE 100 AND 115 VOLT PURIFIER ARE PROTECTED BY A GROUND FAULT INTERRUPTER CIRCUIT GFIC LABCONCO DOES NOT RECOMMEND INSTALLING A GFIC ON THE OUTLET THAT THE PURIFIER PLUGS INTO Service Line Requirements All service lines to the Purifier should be inch outside diameter metal and equipped with an easily accessible shut off valve should disconnection be required If the service line pressure exceeds 40 PSI it must be equipped with a pressure regulator to reduce the line pressure The use of flammable gases or solvents should be avoided in the Purifier Open flames in the cabinet will disrupt the laminar airflow in the cabinet and may damage the HEPA filter Flammable gases or solvents may reach explosive concentrations in the cabinet or ductwork If you feel that your procedure requires the use of an open flame or flammable materials contact the appropriate safety official Chapter 2 Prerequisites The use of air or gases under high pressure should be avoided as they may seriously disrupt the airflow patterns in the cabinet Space Requirements The dimensions for the different models are shown in Appendix B Biosafety Cabinet Dimensions Your Next Step After you have determined that the location you have selected accommodates the installation and operational requirements of your biosafety cabinet you are ready to begin installation Proceed to Chapter 3 Getting Started 11 Chapter 2 Prerequi
6. alarm can be temporarily muted for approximately five minutes by depressing and releasing the alarm silence button Closing the sash back to its operating position will reset the alarm and defeat the muting of the alarm The safety interlock system senses when the sash is closed and will shut off the cabinet blower to prevent it from overheating The interlock also allows the optional ultraviolet UV lamp to operate only when the sash is closed to protect the operator from irradiation 43 Chapter 5 Using Your Purifier 1 To start the Purifier raise the sash until you feel the sash tactile position indicator TPI The TPI functions by increasing the resistance you feel on the sash as it nears its proper operating position Once in position the TPI will set requiring additional upward force to be raised further The correct sash position can be confirmed by aligning the handle of the sash with the indicator decal located on the left side of the opening The indicator decal is shown in Figure 5 1 Figure 5 1 Sash Position Indicator Decal gt 44 Chapter 5 Using Your Purifier 2 Turn the blower switch to the On position as shown in Figure 5 2 The audible and visual alarms will self test and be activated for approximately three seconds The alarms will then shut off If the alarms continue after three seconds recheck the sash position Figure 5 2 A
7. either side wall Orient the bracket so that the center section of the bracket is offset away from the side of the Purifier side wall Secure the bracket using the flat washers lock washers and acorn nuts as shown in Figure 7 2 Slide the other bracket onto the IV bar and install it to the side wall in the same position as the bracket on the other side wall as shown in Figure 7 2 Secure the end brackets by tightening all of the fasteners Figure 7 2 Chapter 7 Modifying Your Biosafety Cabinet 1 Shut off the Purifier and raise the sash to the fully open position 2 Remove the four black protective caps on the side walls and discard them 3 Mount one of the IV brackets on the two studs on either side wall Orient the bracket so that the center section of the bracket is offset away from the side of the Purifier side wall Secure the bracket using the flat washers lock washers and acorn nuts as shown in Figure 7 3 4 Insert the center support brace on the IV Bar Slide the other bracket onto the bar and install it to the side wall in the same position as the bracket on the other wall as shown in Figure 7 3 5 Secure the center support brace and the end brackets by tightening all of the fasteners Figure 7 3 Your Next Step After you understand the maintenance procedures you are ready to proceed to Chapter 8 Troubleshooting 89 Chapter 7 Modifyin
8. 49 Annex G Your Next Step After you understand the maintenance procedures you are ready to proceed to Chapter 7 Modifying Your Biosafety Cabinet Chapter 6 Maintaining Your Purifier 82 7 MobpirviNc Your BiosArETY CABINET There are several ways to modify your Purifier for your individual requirements These include the addition of a UV lamp service fixtures or an intravenous IV bar Installing a UV Lamp All Labconco Purifier Delta Series Safety Cabinets are pre wired for the installation of a UV lamp 4 foot units require the installation of a UV lamp kit consisting of the UV lamp its ballast and fasteners The kit is part number 37450 00 for 115 Volt models 37450 01 for 230 Volt units The UV lamp kit should be installed by a qualified electrician or certifier Completely surface decontaminate all kr interior work surfaces of the Purifier e 2 before beginning the installation of the UV lamp 83 Chapter 7 Modifying Your Biosafety Cabinet T install the UV lamp kit do the following Unplug the unit Using a 7 16 inch wrench or socket remove the two acorn nuts that secure the electronics module to the top of the Purifier as shown in Figure 6 3 Tilt the electronics module back to access the underside of the module Locate the fluorescent lamp ballast in the module The UV lamp ballast mounts next to the fluorescent ballast Using the Phillips sc
9. Before removing the diffuser note its orientation It can only be installed one way Bending or distorting the diffuser may affect the airflow in the cabinet Use care when handling it When properly installed the rear edge of the diffuser should rest on the diffuser support brackets located on the rear wall of the work area as shown in Figure 6 11 1 Shut off the Purifier and raise the sash to the full open position Remove the sash pocket as described in Figure 6 8 2 Remove the fasteners located in the front edge of the supply HEPA filter shelf 3 Carefully lower the diffuser Remove it by tilting the front edge down 4 Install the diffuser plate by reversing the above steps 79 Chapter 6 Maintaining Your Purifier 80 Figure 6 11 Side View of Work Area 7 J Jj Remove the diffuser mounting screws 3 or 4 then swing the front of the diffuser down and then pull it straight out Storage If the Purifier cabinet is to be left unused for more than one month the unit should be prepared for storage The cabinet should not be stored in areas of excess humidity or temperature extremes If the cabinet is moved during storage it must be recertified before use 1 Close the sash completely and seal the bottom edge and the exhaust outlet with plastic sheeting 2 Unplug the unit 3 Ensure that the cabinet will not be moved or disturbed while in storage Decontamination
10. Black vinyl upholstery Shipping weight 35 Ibs 15 9 kg 109 Appendix D Purifier Accessories 110 Adjustable Footrest 37460 00 Elevates feet and permits angle repositioning while in use 18 1 2 w x 11 1 2 d x 8 h Shipping weight 6 16 2 7 kg APPENDIX E Quick For THe DELTA TorAL EXHAUST PURIFIERS Model 36210 36214 Type B2 B2 Sash Height inches 8 8 NSF Listed Downflow FPM 50 60 50 60 Sash Open Area ft 2 69 4 03 Direct Read NSF Listed Inflow FPM 100 110 100 110 Total Exhaust Volume CFM 743 771 1111 1151 Exhaust Alarm Setting Volume CFM 730 1091 Approximate Settings for new Filters Motor Voltage 96 99 85 88 Mag Gauge Reading 0 8 1 1 0 8 1 1 Supply Air Volume Displacement CFM 475 708 Number of Laskin Nozzles Needed 1 1 Supply HEPA Filter Dims in 48x18x3 72x18x3 Exhaust HEPA Filter Dims in 30x18x6 48x18x6 Motor HP 1 3 2 ea 1 3 Speed Winding Used High High Capacitor uF Volts 15 15 Fluorescent Light s F32T8 F72T12 CW TL741 UV Light s G30T8 G64TSL CB Secondary Inflow Method Data Sash Height in 2 97 1 99 Distance Between Points 4 05 4 03 Number of Test Points 11 17 Purifier Correction Factor 1 17 1 23 111 Appendix E Quick Chart for the Delta Purifiers 112 APPENDIX F REFERENCES Many excellent reference texts and booklets are currently available The following is a
11. M SINN 01 13 82 1239402 310M NuB Y Z UL MiB gt mm DD uH1 SSVd 031735 ali ONISNOH 33 18 101 Appendix Biosafety Cabinet Specifications C 2 AH9IJ AV138 H3A04 330 W3AQ iH Q3s010 HSYS 0350712 HSYS TIELE ss3WuvH F a Poe SHOE va 182 3345 NOLINON LG Yolon TWMINHSL 11 3N 3 D 3M IH 09 A8H Y oozesze N d 8 31 YARANA S ONY STVNINH3L WV39VI0 ONIMIM M338138 LJ3NNOJ HOLNNOM OL 310N 9 N 9 g V 7 39unOS 3 9NI NOUdO MyYIY O A SIND 303 134 82 01 T3A 0 L23NNO2 310 MY AJNO 30N343434 HO VOd 310N NiB H 7 102 C 3 Appendix C Biosafety Cabinet Specifications A38 DOPISZE N d NOLON TVNINN3I ZH 09 05 AOOL 7 YIAAYNd l5nr v 03395 BOINOM Hoan S WHINE Nv3ovId ONIMIA d NAOQ 27 3271 9 wNINI3L m O9NOO28VI N33813B H313M 123NNO2 39V110A WOLOM WHOLINOM O4 310N LINN ONIDIAM3S 01 3930n0S YIMOd HOYI 193NNOOSIQ NO dO MYW O M SINN TA T OL 134 92 1934802 ILON 330 330018 035012 HSYS DD6cs N d MSRUDASNYANI an cals NBB Y Z ANO 398383334 Vd 310N 02520 SS3NUVH 6282 N d a 1069 dv 133014 YALI 1SYTWB 10395350115 1 55 Q31v35 34019 103 Appendix Biosa
12. OPTIMUM PERFORMANCE THE UV LAMP SHOULD BE CHANGED ON AN ANNUAL BASIS The UV lamp and the work area of the kr cabinet must be thoroughly e J decontaminated before removing the lamp Start the cabinet and let it operate for 5 minutes Thoroughly surface decontaminate the UV lamp and the work area of the cabinet Unplug the cabinet Raise the sash to its full open position Remove the UV lamp by rotating it 90 degrees and lifting it straight up and out of its sockets Remove the UV lamp by pushing it to the left and swinging it out of the right lamp socket Install new lamp by reversing the removal procedure Resetting a Circuit Breaker To reset any of the circuit breakers located on the front of the electronics module depress the white button until it sets The service operations listed in the rest of this chapter should only be performed by a qualified certifier Downflow Velocities The average downflow velocity for all model Purifiers should be set at 55 5 FPM Chapter 6 Maintaining Your Purifier Downflow Velocity Grid Patterns The downflow velocity test grid for all model Purifiers is listed on the data plate on the lower right side of the airfoil If the decal is missing or obliterated contact Labconco s Product Service Department at 1 800 522 7658 for further details Air Volumes Defined The total exhaust volume is the amount of air that is being exhausted out of the cabine
13. brief listing Primary Containment for Biohazards Selection Installation and Use of Biological Safety Cabinets September 1995 U S Department of Health and Human Services Public Health Service Centers for Disease Control and Prevention and National Institutes of Health Biosafety in Microbiological and Biomedical Laboratories 4th Edition May 1999 U S Department of Health Education and Welfare National Institutes of Health Division of Safety Bethesda MD 20892 Biosafety in the Laboratory 1989 National Research Council National Academy Press Washington D C Laboratory Safety Principles and Practices 2nd Edition 1995 American Society for Microbiology Washington D C National Sanitation Foundation Standard Number 49 for Class II Laminar Flow Biohazard Cabinetry 1992 Ann Arbor Michigan ASHP Technical Assistance Bulletin on Handling Cytotoxic and Hazardous Drugs 1990 American Society of Hospital Pharmacists American Journal of Hospital Pharmacy 47 1033 1049 The Foundations of Laboratory Safety 1990 Stephen R Rayburn Springer Verlag New York 113 Appendix F References Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets U S Department of Health Education and Welfare National Institutes of Health Division of Safety Bethesda MD 20892 Websites of Interest www absa org www cdc gov 114 DECLARATION OF CONFORMITY Application Council Di
14. furniture LABCONCO OFFERS AN OPTIONAL ERGONOMIC CHAIR AND ADJUSTABLE FOOTREST FOR FURTHER INFORMATION SEE Appendix D Purifier Accessories PAY PARTICULAR ATTENTION TO ADJUSTING YOUR POSTURE IN THE AFTERNOON WHEN YOU MAY TEND TO GET FATIGUED WHEN YOU REPOSITION ANY ONE PART OF YOUR BODY YOU MANY NEED TO ADJUST OTHER PARTS AS WELL 1 Don t sit one fixed posture all day Feet Knees and Legs Make sure your feet can rest solidly and comfortably on the floor or footrest while sitting Use an adjustable chair that allows your feet to rest firmly on the floor or use a footrest If you use a footrest be sure it is wide enough to accommodate different leg positions within your comfort zone gt REST YOUR FEET FIRMLY ON THE FLOOR OR A FOOTREST Don t dangle your feet and compress your thighs Chapter 5 Using Your Purifier Providing Enough Leg Room Be sure you have sufficient space under your work surface for your knees and legs Avoid concentrated pressure points along the underside of your thigh near the knee and the backside of your lower leg Stretch your legs and vary your leg posture throughout the day Leg Comfort Vary your leg positions throughout the day Avoid placing boxes or other items under the cabinet that limit your leg room You should be able to pull your chair all the way up to your Purifier without interference Walk Get up from your Purifier periodically and
15. silicon sealant to this surface of the connector and align the three mounting holes with the matching holes on the bottom surface of the Purifier amp Initial Certification Prior to use all Purifier cabinets should be certified by a qualified certifier Under normal operating conditions the Purifier cabinet should be recertified at least annually and when moved or serviced The certifier should perform the following tests as recommended in NSF International Standard Number 49 Chapter 3 Getting Started Downflow Velocity Profile Test Inflow Velocity Test Airflow Smoke Patterns HEPA Filter Leak Test Electrical Leakage and Ground Circuit Resistance Test Vibration Test Noise Level Test Lighting Intensity Test Cabinet Leak Test These tests are user comfort related tests and may be omitted at the user s or certifier s discretion Because of the complexity of this test it may be performed when deemed necessary by the customer and certifier If you have any questions regarding certification agencies or need assistance in locating one contact Labconco s Product Service Department at 1 800 522 7658 or 816 333 8811 Your Next Step After your Purifier has been installed and certified you are ready to proceed to Chapter 4 Theory Of Operation And Safety Precautions Chapter 3 Getting Started 30 4 THEORY OF OPERATION AND SAFETY PRECAUTIONS
16. take brief walks Back Use your chair to fully support your body Distribute your weight evenly and use the entire seat and backrest to support your body If your chair has adjustable low back support match the contours of the chair s backrest to the natural curve of your lower spine Always make sure your lower back is well supported Make sure it feels comfortable in the position in which you are working DISTRIBUTE YOUR WEIGHT EVENLY AND USE THE ENTIRE SEAT AND BACKREST TO SUPPORT YOUR BODY mI Don t slouch forward Adjust often If your chair is adjustable experiment with the adjustments to find numerous comfortable positions then adjust the chair frequently Chapter 5 Using Your Purifier someone else don t assume the settings are properly set for you If you are using a chair for the first time or if you share a chair with Forearms Wrists and Hands Keep your forearms wrists and hands aligned in a straight natural position Avoid bending or angling your wrists while working Don t Anchor Your Wrists When working do not anchor or rest your wrists on the work surface Resting your palms while working may be harmful because it can cause you to bend your wrists back and can apply pressure to the undersides of your wrists MAINTAIN A STRAIGHT NATURAL lt gt WRIST POSITION WHILE WORKING Don t rest your palms on the work surface while working Don t anchor your wrists thi
17. tubing connector onto the coupling until it can no longer be turned by hand Holding the coupling secure with a wrench or pliers turn the tube fitting approximately one more revolution using pliers or a wrench Orient the tube fitting such that it is easy to access it with the incoming service tube 9 Connect the service tube to the tube fitting by pressing the tube all the way into the fitting turning the tubing nut until hand tight and then approximately one more turn using pliers or a wrench Make sure the service tubing does not obstruct the wiring or sash weights or cables 10 Make sure the service fitting in the Purifier is in the off position before pressurizing the service line Once pressurized check the valve body the coupling and the tube fitting for leaks Tighten any leaking joints 11 Reattach the side panel Chapter 7 Modifying Your Biosafety Cabinet 88 12 The valve is shipped with a white plastic index disc This disc can be replaced with the orange or yellow disc supplied by unscrewing it and replacing it with the new one Installing the IV Bar yon Completely surface decontaminate all interior work surfaces of the Purifier N before beginning the installation of the IV bar Shut off the Purifier and raise the sash to the fully open position Remove the four black protective caps on the side walls and discard them Mount one of the IV brackets on the two studs on
18. Exhaust HEPA Filter l Loosen and remove all of the Phillips head screws on the exhaust HEPA filter cover Gently rock the cover and pull it away from the unit Using a inch wrench loosen the four exhaust filter clamping bolts located on the top of the unit as shown in Figure 6 9 Loosen all of the bolts until the filter drops away from the top of the Purifier Slid the exhaust HEPA filter straight out of the unit With the exhaust filer out of the unit inspect the filter for damage Chapter 6 Maintaining Your Purifier To Install an Exhaust HEPA Filter 1 Slide the new exhaust HEPA filter all the way into the shelf 2 Sequentially tighten the four exhaust filter clamp bolts as shown in Figure 6 9 until the exhaust filter gasket is compressed approximately 50 proper seal of the exhaust HEPA The exhaust filter sealing bolts should only be tightened enough to ensure a filter At maximum tightness the exhaust filter s gasket should be compressed to 50 of its original thickness NEVER tighten the bolts beyond this setting Install the exhaust filter chamber cover uniformly tightening the screws on the cover until the gasket has formed a proper seal Install the sash pocket Install the front dress panel Plug in the cabinet and have it recertified before use Figure 6 9 Exhaust Filter Clamp bolts 4 These bolt heads are visible on the outside edges of the exhaust damper conne
19. Gaseous decontamination should only be performed by trained personnel HN The unique sash pocket of the Purifier allows for the gaseous decontamination of the entire interior of the cabinet as well as the inside of the sash and the outside Chapter 6 Maintaining Your Purifier surface of the blower cover an area which may possibly become contaminated during operation To seal the Purifier follow these steps 1 Remove the front dress panel as shown in Figure 6 2 Place decontamination equipment inside the work area of the Purifier Remove the sash stops from the lower front edge of the unit Close the sash until it covers the lower edge of the unit The outlets on the interior of the Purifier are energized as long as the Purifier is plugged in Unplug the Purifier before decontamination equipment is plugged into these outlets 2 Seal the air inlet on the top of the cabinet Ensure that all service valves and the drain valve are closed 3 Using a flexible sealant plug the area around the sash cables where they exit the top of the sash pocket 4 Use duct tape to seal the outside edge of the sash pocket to the side panels 5 Seal the upper and lower edges of the sash pocket using duct tape 6 Seal the sides of the sash to the corner posts and the sash handle to the lower front edge of the unit 7 Close the exhaust damper completely 8 Perform the decontamination as described in NSF Standard
20. ING AND RETURN THE SPEED CONTROLLER TO ITS PREVIOUS VALUE Use of the Access Tube For DOP Testing w THE PURIFIER UTILIZES A UNIQUE ACCESS TUBE FOR SAMPLING THE UPSTREAM CONCENTRATION OF DOP FOR THE SUPPLY FILTER IF AFTER READING THESE INSTRUCTIONS YOU HAVE FURTHER QUESTIONS PLEASE CONTACT LABCONCO S PRODUCT SERVICE DEPARTMENT The access tube may be surface contaminated DO NOT access this line until the outside surface has been decontaminated DOP scanning of the HEPA filters should only be performed by a qualified certification technician as part of the recertification procedure 1 Remove the work surface as shown in Figure 6 1 2 Locate the access tubes on left side of the rear plenum wall behind the towel catch 3 Pull the tube toward the front of the unit Remove the access tube s cover 4 Using an appropriate photometer connect the unit s upstream line to the access tube 5 After completing the DOP test of the filters replace the access tube cover ensuring that it is sealed properly 6 Replace the access tube and the work surface Chapter 6 Maintaining Your Purifier 74 HEPA Filter Replacement A The cabinet must be properly decontaminated before servicing the e J exhaust HEPA filter The HEPA filters should only be serviced by a qualified certification technician Following replacement of a HEPA filter the cabinet MUST be recertified by a qualified certification t
21. J cabinet into normal operation or the alarm activates frequently DO NOT continue to use the Purifier NEVER OVERRIDE THE ALARM RESET MECHANISM TO CONTINUE WORKING IN THE CABINET You MUST correct the problem in the exhaust system before resuming operations Working In Your Purifier Planning e Thoroughly understand procedures and equipment required before beginning work e Arrange for minimal disruptions such as room traffic or entry into the room while the cabinet is in use Start up e Turn off UV light if included e Slowly raise the sash until the TPI engages at the proper height Confirm the sash is at its proper height by examining the sash indicator decal located on the left side of the work area Turn on fluorescent light and cabinet blower e Check the return air grilles for obstructions and note the pressure gauge reading e Allow the cabinet to operate unobstructed for 5 minutes e Wash hands and arms thoroughly with germicidal soap e Wear a long sleeved lab coat with knit cuffs and over the cuff rubber gloves Use protective eyewear Wear a protective mask if appropriate Wipe Down e Raise the sash to its full open position approximately 19 inches open Mute the alarm by depressing the Alarm Silence switch Chapter 5 Using Your Purifier Wipe down the interior surfaces of the cabinet with 70 ethanol or a suitable disinfectant and allow to dry Loading Materials and Equipment On
22. LARA I Dic TCR DELIA SERIES The Total Exhaust Purifier Cabinets are equipped with an alarm system that monitors the volume of air exhausting from the cabinet If the exhaust volume drops below the minimum requirement the following OCCUIS 45 Chapter 5 Using Your Purifier An audible alarm sounds The red Alarm indicator light illuminates The cabinet blower supplying the downflow air in the work area shuts off 4 e WwW During an alarm the cabinet blower shuts off to prevent air from escaping the work area of the cabinet If the alarm reactivates after five seconds DO NOT continue to use the Purifier NEVER OVERRIDE THE ALARM RESET SWITCH TO CONTINUE WORKING IN THE CABINET You MUST correct the problem in the exhaust system before resuming operations If The Alarm Activates The most common causes of alarm activation are e Mechanical failure of the external blower An obstruction or leak in the exhaust system e Loading of the exhaust HEPA filter e If the alarm sounds during use immediately take appropriate action to prevent contamination to you and other personnel in the area Resetting The Alarm System Once the cause of the face velocity alarm has been determined and corrected the alarm may be reset by depressing the Alarm Silence switch Chapter 5 Using Your Purifier Ae IMPORTANT If pressing the Alarm Silence switch does not reset the e
23. O S PRODUCT SERVICE DEPARTMENT FOR FURTHER DETAILS 1 Chapter 6 Maintaining Your Purifier Remove the access panel located on the front of the electronics module by removing the right hand Phillips screw and loosening the left hand screw as shown in Figure 6 5 Pivot the panel out of the way Locate the voltage test points on the electrical panel The test points are identified by Figure 6 6 shown below Using a voltmeter and probes measure the desired voltage by connecting the voltmeter probes to the appropriate points Figure 6 5 Remove the right side screw and loosen the left one Then rotate the cover out of the way wee Mp ER E Voltage test points I Blower speed decrease button Blower speed increase button Figure 6 6 These two leads measure the line voltage These two leads measure the motor voltage 71 Chapter 6 Maintaining Your Purifier Speed Control Adjustment The Purifier blower speed should only be adjusted by a qualified certification technician Adjustment of the blower speed will affect the containment efficiency of the unit The Purifier s blower speed is adjusted and its setpoint is maintained by a solid state electronic circuit There is no potentiometer as on traditional biosafety cabinets The blower speed setting is adjusted and protected by an electron
24. ON OF LABCONCO UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED IF YOUR CABINET WAS DAMAGED IN TRANSIT YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES Do not discard the carton or packing material for your Purifier until you have checked all of the components and installed and tested the unit Do not remove the Purifier from its shipping skid until it is ready to be placed into its final location Move the unit by placing a flat low dolly under the shipping skid or by using a floor jack Do not move the cabinet by tilting it onto a hand truck Chapter 3 Getting Started Purifier Components Labconco manufactures biosafety cabinets available in 4 foot and 6 foot widths Some models are available in 115V 100V or 230V Locate the cabinet model you received in the following group of tables Verify that the components listed are present and undamaged Catalog Purifier Description 36210 00 4 foot Purifier 8 inch sash opening 115 VAC 36210 04 4 foot Purifier with UV lamp amp fixture 8 inch sash opening 115 VAC 36210 10 4 foot Purifier 8 inch sash opening 100 VAC 50 60 Hz 36210 14 4 foot Purifier with UV lamp and fixture 8 inch opening 100 VAC 50 60 Hz 36210 20 4 foot Purifier 8 inch sash opening 230 VAC 36210 24 4 foot Purifier with UV lamp amp fixture 8 inch sash opening 230 VAC 36214 00 6 foot Purifier 8 in
25. SSIVE NUMBER OF ELBOWS TO CLEAR OTHER ITEMS FOR A FURTHER DISCUSSION OF THE CABINET S EXHAUST SYSTEM REQUIREMENTS PLEASE GO TO Chapter 3 Getting Started Electrical Requirements The different Purifier models have the following electrical requirements Table 2 1 Model Requirements 36210 00 09 115 VAC 60 Hz 12 Amps 36210 10 19 100 VAC 50 60 Hz 12 Amps 36210 20 29 230 VAC 50 Hz 6 Amps 36214 00 09 115 VAC 60 Hz 12 Amps 36214 10 19 100 VAC 50 60 Hz 12 Amps All Purifiers with model numbers ending in 00 to 09 are designed for operation at 115 volts 60Hz alternating current Models with numbers ending in 10 to 19 are designed for operation at 100 to 120 volts 50 60Hz Purifiers with model numbers ending in 20 to 29 are designed for operation at 230 20 volts 50Hz alternating current A dedicated outlet with a circuit breaker rated at 20 amps should be located as close as possible to the right rear side of the cabinet at Chapter 2 Prerequisites Chapter 2 Prerequisites 10 a height even with or higher than the bottom of the cabinet in its final location If your electrical outlet is distant from this location contact Labconco s Product Service Department for information on longer cords Always follow the plug manufacturer s instructions for the proper assembly and testing of the plug and power cord BOTH ELECTRICAL OUTLETS OF
26. TY CABINET Installing a UV Lamp Installing Additional Service Fixtures Installing the Bar Your Next Step CHAPTER 8 TROUBLESHOOTING APPENDIX A PURIFIER COMPONENTS APPENDIX B BIOSAFETY CABINET DIMENSIONS APPENDIX C BIOSAFETY CABINET SPECIFICATIONS Electrical Data Motor Specifications APPENDIX D PURIFIER ACCESSORIES Safety Orange Air Foil Base Stands Service Valve Kit Ultraviolet Lamp Kits IV Bar Kits Remote Blower Ergonomic Chair Adjustable Footrest APPENDIX E QUICK CHART FOR THE DELTA TOTAL EXHAUST PURIFIERS APPENDIX F REFERENCES DECLARATION OF CONFORMITY 83 83 86 88 89 91 95 97 99 99 99 107 107 107 108 108 108 109 109 110 111 113 115 CHAPTER 1 INTRODUCTION Congratulations on your purchase of a Labconco Purifier Delta Series Total Exhaust Biosafety Cabinet Your Purifier is designed to protect you your product and your laboratory environment from biohazardous aerosols It is the result of Labconco s more than 25 years experience of manufacturing biohazard cabinetry and many of its features were suggested to us by users like you The Purifier offers many unique features to enhance safety performance and ergometrics To take full advantage of them please acquaint yourself with this manual and keep it handy for future reference If you are unfamiliar with how biosafety cabinets operate please review Chapter 4 Theory of Operation and Safety Precautions before y
27. aining your fingers to relax when they become tense e Do you use your whole arm to reach for objects not located near your fingers Is your work positioned directly in front of you e Are your wrists straight and your shoulders relaxed e Have you eliminated glare and bright reflections on the sash without compromising your posture Chapter 5 Using Your Purifier General Prevention Do you take breaks and walk around briefly preferably once per hour Do you exercise regularly Periodically do you take inventory of the stress in your life and change what is within your control to change Your Next Step After you understand how to operate and work in the Purifier you are ready to proceed to Chapter 6 Maintaining Your Purifier Chapter 5 Using Your Purifier 58 6 MAINTAINING YOUR PURIFIER Now that you have an understanding of how to work in the Purifier we will review the suggested maintenance schedule and the common service operations necessary to maintain your Purifier for peak performance Many of the service operations should be performed only by trained and experienced certification technicians after the cabinet has been properly decontaminated DO NOT attempt to perform these operations if you are not properly trained The service operations that require qualified certifiers are preceded by the wrench icon Routine Maintenance Schedule Weekly e Using 70 ethano
28. aminants from the work area Unloading Materials and Equipment e Objects in contact with contaminated material should be surface decontaminated before removal from the cabinet e All open trays or containers should be covered before being removed from the cabinet Wipe Down e Wipe down the interior surfaces of the cabinet with 70 ethanol or a suitable disinfectant and allow to dry e Periodically lift the work surface and wipe down the area beneath it e Inspect and clean the towel catch located at the rear of the work area beneath the work pan e Dispose of rubber gloves appropriately and have lab coat laundered properly e Wash hands and arms thoroughly with germicidal soap Shutdown e Turn off the fluorescent light and cabinet blower close the sash and turn on the UV light if appropriate Safety and Comfort Many factors in our work environment determine whether we work efficiently and in a manner that promotes good health and safety By considering acting on and periodically reevaluating the recommendations in this section it is possible to create a safer more comfortable healthful and efficient work environment Chapter 5 Using Your Purifier Working intensely or for a long time in uncomfortable or unnatural positions poses risks Organizing Your Adjustments The order in which you make various adjustments to your body position and work area may vary depending on the adjustability of your
29. ble for indirect consequential or special damages of any kind This statement may be altered by a specific published amendment No individual has authorization to alter the provisions of this warranty policy or its amendments Lamps and filters are not covered by this warranty Damage due to corrosion or accidental breakage is also not covered Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various federal state or local regulations All users of this equipment are required to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Labconco Corporation is held harmless with respect to user s compliance with such regulations Part 3758400 REV B ECO B016 TABLE Or CONTENTS CHAPTER 1 INTRODUCTION About This Manual Typographical Conventions Your Next Step CHAPTER 2 PREREQUISITES Location Requirements Exhaust Requirements Electrical Requirements Service Line Requirements Space Requirements Your Next Step CHAPTER 3 GETTING STARTED Unpacking Your Biosafety Cabinet Purifier Components Removing the Shipping Skid Installing the Purifier on an Existing Work Surface Installing the Purifier on the Labconco Base Stand Exhaust System Connections Exhaust System Requirements Connecting the Purifier to Service Lines Measuring the Total Exhaust V
30. ch sash opening 115 VAC 36214 04 6 foot Purifier with UV lamp amp fixture 8 inch sash opening 115 VAC 36214 10 6 foot Purifier 8 inch sash opening 100 VAC 50 60 Hz 36214 14 6 foot Purifier with UV lamp and fixture 8 inch opening 100 VAC 50 60 Hz Plus the Following Part Component Description 37584 00 User s Manual Drain Valve Assembly Power Cord If you did not receive one or more of the components listed for your Purifier or if any of the components are damaged contact Labconco Corporation immediately for further instructions Removing the Shipping Skid LEAVE THE PURIFIER ATTACHED 5 TO ITS SHIPPING SKID UNTIL IT IS AS CLOSE ITS FINAL LOCATION AS POSSIBLE MOVE THE UNIT BY USING A SUITABLE FLOOR JACK OR BY PLACING A 15 Chapter 3 Getting Started FURNITURE DOLLY UNDERDNEATH THE SKID DO NOT MOVE THE CABINET BY TILTING IT ONTO A HAND TRUCK After you verify the Purifier components move your cabinet to the location where you want to install it Then follow the steps listed below to remove the shipping skid from your unit To remove the shipping skid The side panels of the Purifier are 1 heavy exercise caution when removing and installing them 1 Remove the plastic wrapping and corner posts Remove the packaging material from the top of the cabinet 2 Remove both side panels by loosening and keeping the screws and 3 acorn nuts that secure each panel to the Purifier a
31. control system should only be adjusted by a qualified certification technician as part of the recertification procedure 1 To adjust the total exhaust volume locate the exhaust damper on the top of the cabinet 2 Lock the damper in place using the attached wing nut when the proper total exhaust volume is obtained as shown in Table 6 1 Chapter 6 Maintaining Your Purifier Measuring the Cabinet Inflow Volume Primary Method The average inflow velocity for all Purifiers is 105 5 FPM and should be determined by converting the inflow volume to the average inflow velocity The inflow volume is measured directly by sealing a direct inflow measuring instrument to the face of the unit When corrected for local conditions dividing the inflow volume by the opening area will yield the average inflow velocity as shown in table 6 2 Table 6 2 Sash Avg Inflow Work Inflow Purifier Height Velocity Opening Volume Model inches FPM Area sq ft CFM 36210 8 0S 5 269 269 296 36214 8 1055 403 403 43 Inflow Velocity Calculation Secondary Method If the primary method cannot be performed the secondary inflow calculation method should be performed The secondary method uses a hot wire thermoanemometer In this method the sash is lowered until the open area of the sash equals one square foot and a series of velocity readings are taken at the front of the unit 1 For each
32. ctor Loosen all four at least inch 13 mm Chapter 6 Maintaining Your Purifier Motor Blower Maintenance and Replacement The motor blower should be serviced by a qualified certification technician The cabinet blower motor s bearings gt are sealed and require no lubrication DO NOT attempt to lubricate them To replace the Motor Blower 1 Unplug the cabinet 2 Remove the front dress panel as shown in Figure 6 2 3 Remove the sash pocket as shown in Figure 6 8 4 Loosen and remove all of the Phillips screws on the blower chamber cover Gently rock the cover slide 1t to the left or right and swing it out and away from the unit 5 Using screwdriver disconnect the motor ground wire from the blower housing 6 Disconnect the motor leads from the wiring harness by pulling apart each of the 4 connectors 7 Using a 1 2 inch wrench remove the two upper and one lower blower mounting bolts as shown in Figure 6 10 The motor blower assembly is heavy 1 Handle with 8 While supporting the blower assembly pull the assembly straight out the plenum 9 replace the motor blower assembly reverse the above procedure 10 Plug in the cabinet and have it recertified before use Chapter 6 Maintaining Your Purifier Figure 6 10 Diffuser Removal A The work area of the cabinet must be thoroughly disinfected before e J removing the diffuser
33. e four available service fixture holes in both side walls The service fixture kit is Labconco part number 36933 00 To install the service fixture 1 Decide into which of the available locations you want to install the service fixture 2 Remove the side panel on that side of the unit as shown in Figure 3 2 3 Remove as much of the silicone sealant as possible from around the outside of the service fixture hole plug 4 Placing a block of wood or plastic against the outside edge of the plug and using a small hammer gently tap the plug out of the hole Chapter 7 Modifying Your Biosafety Cabinet Do not use excessive force in attempting to remove the hole plug Damage to the side wall of the cabinet could occur 5 Carefully remove any remaining sealant from both sides of the service fixture hole 6 Using the Teflon tape supplied wrap two to three turns of tape on the male pipe thread of the valve and the tube fitting 7 Put the threaded end of the fixture through the hole orienting the valve as shown in Figure 3 7 Using the silicone sealant apply a bead of silicone on the outside surface of the cabinet side around the valve body This will provide a pressure tight seal around the valve body Place the two washers on the valve thread then install the coupler Holding the valve body in position use a wrench or pliers to thread the coupling on until the valve is captured on the side panel 8 Thread the
34. e moving items keep your wrist arm and shoulder free to move Aligning Forearms Wrists and Hands When working in the Purifier keep your forearms wrists and hands comfortably aligned with each other in a straight natural line Avoid bending or angling your wrists Taking Breaks and Varying Your Tasks As noted earlier your furniture placement lab equipment and lighting are only a few of the factors that determine comfort Your work habits are also very important Remember the following e Take breaks e When you work at your Purifier for long periods take short breaks at least once per hour and preferably more often You may find that frequent short breaks will benefit you more than fewer longer breaks e If you find that you forget to take breaks use a timer e During your breaks stand up and stretch especially any muscles and joints you may have held in an extended static posture while working Vary Your Tasks Examine your work habits and the types of tasks you perform Break up the routine and try to vary your tasks during the day By doing so you may avoid sitting in one position or performing the same activities continuously for several hours using your hands arms shoulders neck or back Reduce Sources of Stress Take an inventory of things at work that are stressful to you If you perceive that your physical or psychological health is being affected take time to evaluate what changes yo
35. e exhaust system Loading of the exhaust HEPA filter HEPA filter loading Blockage of the return air slots or grille Blockage of the exhaust outlet Blockage or restriction under the work surface CORRECTIVE ACTION Inspect lamp wiring Replace lamp ballasts Ensure that roof blower is working Ensure that the exhaust damper is properly set by a qualified certifier Confirm that the roof mounted blower meets the volume and pressure requirements in the Airflow Data Table Inspect the exhaust system Inspect the exhaust system Inspect the exhaust system Replace the HEPA filters The pressure reading will steadily increase as the unit is used Check all return air slots and grilles to ensure that they are not blocked or restricted Ensure that the exhaust outlet is not blocked or restricted Ensure that the plenum beneath the work surface is unobstructed Chapter 8 Troubleshooting PROBLEM Contamination of work in the cabinet CAUSE Improper technique or procedure for the biohazard cabinet Restriction of the return air slots or grille blockage of the exhaust outlet External factors are disrupting the cabinet airflow patterns or acting as a source of contamination Cabinet is out of adjustment HEPA filter s are defective CORRECTIVE ACTION See Use of the Cabinet section in the manual Ensure that all return air slots grilles and the
36. e time to eliminate glare and reflections To control daylight use blinds shades or drapes or try other glare reducing measures Use indirect or reduced lighting to avoid bright spots on the sash If glare is a problem consider these actions e Turn off or reduce ceiling lights and use task lighting one or more adjustable lamps to illuminate your work Avoid compromising your posture to compensate for glare or reflections Chapter 5 Using Your Purifier Positioning Work Materials Position work materials directly in front of you to avoid twisting your neck and torso This makes it possible to work with your shoulders relaxed and your upper arms hanging freely at your sides Minimizing Your Reach Arrange your frequently used materials to minimize the distance you reach for them Hand Manipulation Notice how you use your fingers and hands while working Avoid keeping any unnecessary tension in your thumbs and fingers Observe whether you have a heavy touch or a light touch while working Reaching for Materials To reach material that is not near your hands move your whole arm avoid stretching your fingers and angling your wrists RELAX YOUR THUMBS AND FINGERS WHILE WORKING NOTICE AND RELEASE EXCESS TENSION Don t work with unnecessary tension in your thumbs and fingers Wrist Position Use your whole arm and shoulder to move objects not just your wrist Don t rest or anchor your wrist whil
37. echnician 1 Unplug the cabinet 2 Remove the front dress panel as shown in Figure 6 2 3 Remove the sash pocket by loosening the two Phillips screws on the left side of the pocket Support the sash pocket while removing the two screws on the right side of the sash pocket Swing the sash pocket out and away from the unit as shown in figure 6 7 Figure 6 7 Loosen these two screws Then remove these two screws while supporting the pocket Then swing the sash pocket out Chapter 6 Maintaining Your Purifier To Remove the Supply HEPA Filter The supply HEPA filter is awkward to handle and heavy Use appropriate lifting techniques to remove and handle it Use two people to remove the filter if possible 1 Loosen and remove all of the Phillips screws on the blower chamber cover Gently rock the cover slide it to the left or right and swing it out and away from the unit 2 Using a 1 2 inch wrench loosen the four locking bolts that contact each corner of the plenum box at least 1 2 inch as shown in Figure 6 8 3 Use a 1 2 inch socket or wrench to tighten the three plenum lift bolts located as noted in Figure 6 8 This will lift the plenum box off the supply HEPA filter 4 Slide the supply HEPA filter straight out the unit 5 With the HEPA filter removed inspect the filter shelf for damage Figure 6 8 Plenum Lift Bolts 3 These bolts pass through the side brack
38. efore HEPA filter changes Before performing performance tests requiring entry into contaminated areas Before moving the cabinet Before changing research programs After the gross spill of biohazardous material THE PROCEDURES FOR PERFORMING A GASEOUS DECONTAMINATION ARE THOROUGHLY OUTLINED IN THE U S DEPARTMENT OF HEALTH EDUCATION AND WELFARE BOOKLET ENTITLED Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets WHICH IS AVAILABLE FROM THE NATIONAL INSTITUTES OF HEALTH DIVISION OF SAFETY BETHESDA MARYLAND 20892 OR FROM LABCONCO CORPORATION Chapter 4 Theory Of Operation And Safety Precautions Your Next Step After you understand the theory of operation and safety precautions you are ready to proceed to Chapter 5 Using Your Purifier Chapter 4 Theory Of Operation And Safety Precautions 42 Usina Your PURIFIER Operating the Sliding Sash Because of the Purifier s counterbalanced anti racking sash mechanism it will take only a few pounds of force to move the sash up or down and you can operate the sash smoothly with one or two hands positioned any where along the handle The sash position alarm and safety interlock system sense the sashes position and act appropriately Your Purifier is designed to operate with an 8 inch sash opening Raising the sash above its operating height will activate the audible and visual alarms The audible
39. ermine flow rates Table 6 5 Model Numbers Purifier Correction Factors FPM TO CFM 36210 1 17 360310 IT 36214 67 Chapter 6 Maintaining Your Purifier 68 9 Calculate the average of all the flow rates 10 The resulting value is the inflow volume in cubic feet per minute CFM In order to convert this value to the average inflow velocity of feet per minute FPM divide the volume by the sash open area given in Table 6 5 below Table 6 6 Model Numbers Sash Open Area square feet 36210 2 69 36214 11 The resulting value will be the estimated average inflow velocity in FPM Calibration of the Face Velocity Alarm qualified certification technician should calibrate the Face Velocity Alarm The alarm switch is set at the factory at its lowest pressure setting and it will need to be calibrated before using the Purifier Set the exhaust damper so that the exhaust volume is at the alarm setpoint as indicated in Table 6 1 and mark the damper position With the Purifier running remove the hole cover and turn the adjusting screw as shown in Figure 6 3 very slowly clockwise increasing the pressure setting until the alarm is activated This is the alarm setpoint Confirm the alarm operation by opening the damper slightly and pressing the Alarm Silence switch The cabinet will operate normally While the cabinet is running slowly return the damper to
40. ets and are threaded into the plenum box Tighten all three to lift the box at least 1 4 inch 6 mm Locking bolts 4 These bolts are threaded through the side brackets and their ends contact tlie corners of the plenum box Loosen all four at least 1 inch 13 mm 75 Chapter 6 Maintaining Your Purifier To Install a Supply HEPA Filter 1 Install a new supply HEPA filter by pushing it straight into the cabinet ensuring that it is correctly oriented and that the filter is centered on the filter shelf Use a 1 2 inch socket or wrench to sequentially loosen the three plenum lift bolts located as noted in Figure 6 8 When properly positioned the plenum box will be aligned with the supply HEPA filter frame and there will be an approximately 1 4 inch gap between the bottom of the plenum lift bolt head and the top of the lift bracket This will allow the plenum box to properly seat as the filter gasket is compressed Using a 1 2 inch wrench tighten the four locking bolts located at each corner of the plenum box as shown in Figure 6 8 until the supply HEPA filter gasket is compressed to approximately 50 of its thickness The locking bolts should only be tightened enough to ensure proper seal of the supply HEPA filter At maximum tightness the supply filter s gasket should be compressed to 50 of its original thickness NEVER tighten the locking bolts beyond this setting To Remove the
41. exhaust outlet are unobstructed See Installation section of this manual Have cabinet recertified Chapter 8 Troubleshooting 94 APPENDIX PURIFIER COMPONENTS Illustration A 1 indicates the location of the following service parts Purifier Replacement Parts Item Quantity Part No Description 1 1 3734000 Side Panel 2 1 3734001 Side Panel L H 3 1 5950000 Exhaust HEPA Filter 4 foot 3A 1 5960000 Exhaust HEPA Filter 6 foot 4 1 3731100 Motor Blower Assembly 115 VAC 4 foot 4A 1 3731101 Motor Blower Assembly 115 VAC 6 foot 4B 1 3757100 Motor Blower Assembly 100 VAC 4 foot 4C 1 3757101 Motor Blower Assembly 100 VAC 6 foot 4D 1 3751900 Motor Blower Assembly 230 VAC 4 foot 5 1 1211200 Motor 115 VAC 4 and 6 foot 5A 1 1210503 Motor 100 VAC 4 foot 5B 1 1210501 Motor 100 VAC 6 foot 5C 1 1210502 Motor 230 VAC 4 foot 6 1 3739401 Supply HEPA Filter 4 foot 6A 1 3739402 Supply HEPA Filter 6 foot 7 1 9721902 Lamp Fluorescent 4 foot TA 1 1278400 Lamp Fluorescent 6 foot 8 1 1271300 Lamp UV 4 foot not shown 8A 1 1231900 Lamp UV 6 foot not shown 9 2 1287900 Receptacle Cover 95 Appendix A Purifier Components A 1 96 B BIoSAFETY CABINET DIMENSIONS B 1
42. f Operation And Safety Precautions Figure 4 1 Filter Frame A Separator 2 2 Gasket Seal Z Adhesive Bond Z Continuous Between Filter Pack 4 Sheet of and Integral Frame Flat Filter Medium HEPA Filter Laminar Airflow Laminar airflow is defined as the movement of a body of air in a single direction with a uniform velocity In practice the laminar downflow of air in the cabinet captures any aerosol generated in the work area of the cabinet and directs it to the HEPA filters In order to be true laminar downflow a number of individual downflow velocity test points The Downflow Velocity Profile must be 20 of the average of all the test points This is shown graphically in Figure 4 2 33 Chapter 4 Theory Of Operation And Safety Precautions 34 Figure 4 2 Directional Airflow Directional airflow also plays a key role in biosafety cabinet performance Air is drawn into the front of the cabinet at the front grille This curtain of air makes it more difficult for aerosols to escape out of the work area of the cabinet and into the outside environment This airflow is often calculated and referred to as the Inflow Volume or Average Inflow Velocity This is shown graphically in Figure 4 3 Chapter 4 Theory Of Operation Safety Precautions Figure 4 3 Motor Blower The motor blower assembly pulls air into the top of the cab
43. fety Cabinet Specifications C 4 15 03395 VOLNOM 034 8Y9I O0GZG F N d W3RWOJSNYMI dh JILE M 00 4j os fh ma r V n S1H50 93A018 JANO HOIOM 1YNiNUS V1 3NI S TYNWH31 27 3 7 9 TVNI H31 S ONY STWNINYSL N33M138 YILIN LJ3NNOJ BSOVITOA OLOM 301INOM Qi 310N NOUO 8 A3u D0g85 N d 09 05 9 31 WYYOVIG SNI3JIM O09NO28VI 3ounos u3MOd N 09910 SINN 904 734 97 01 134 62 1234W03 310N 37360 lll LABCONCO E UNON OLOY AVS 104 Appendix Biosafety Cabinet Specifications C 5 1HA 8 LI zi 1331 SLINO 330 M38038 0935019 HSYS Wig 8fi 1HA 2Z pd 03305 NOLINOM 100 VOLON 9 TYNIN331 ONY SIYNIMU3 N33M138 S313 123NNO2 39V170A YOLOM YOLINOM Ol 310N 8 A38 N d LI 387 S TWNINY3L OS AOLZ 7 WE YIAN NvY32VIO 9NIMIM OONOO28V I JANOS 43MOd MONI LO3NNOOSIQ NOSO D M SINN 304 VWALE OL 134 92 1234809 73104 AWO X04 Vd 310N SSINYYH PELE N d A1SvVTIYB 1N325330n14 NSH1 SS d q31v3s ONISNOH 8380 18 105 Appendix Biosafety Cabinet Specifications 106 APPENDIX D PURIFIER ACCESSORIES Safety Orange Air Foil Bold orange color cautions the operator to avoid placing mate
44. g Your Biosafety Cabinet 90 CHAPTER 8 TROUBLESHOOTING Refer to the following table if your Purifier fails to operate properly If the suggested corrective actions do not solve your problem contact Labconco for additional assistance PROBLEM Cabinet blower and lights won t turn on Blower won t turn on but lights work Cabinet blower turns on but lights don t work CAUSE Unit not plugged into outlet Circuit breakers tripped Sash closed Blower wiring is disconnected Blower motor is defective Lamp not installed correctly Lamp is defective Lamp circuit breaker tripped CORRECTIVE ACTION Plug the Purifier into appropriate electrical service Check connection to control box on top of unit Reset circuit breakers Raise sash Inspect blower wiring Replace blower motor Inspect lamp installation Replace lamp Reset the lamp circuit breaker 91 Chapter 8 Troubleshooting PROBLEM Cabinet blower turns on but lights don t work cont Alarm sounds after the alarm system switch is released from Reset Slight increase in pressure reading CAUSE Lamp wiring is disconnected Defective lamp ballasts Roof blower is off Exhaust damper is improperly positioned Remote blower is improperly sized Mechanical failure of the exhaust system Obstruction in the exhaust system Leak or additional load on th
45. g the unit When installing the Purifier onto an existing work surface or benchtop ensure that the structure can safely support the combined weight of the cabinet and any related equipment The work surface should be at least as wide as the unit and 31 inches deep to properly support the unit Chapter 3 Getting Started Chapter 3 Getting Started The work surface should be smooth and durable The surface should be nonporous and resistant to the disinfectants and chemicals used in conjunction with the Purifier These criteria will allow a proper seal to form between the bottom of the Purifier and the work surface A hole or notch may need to be cut in some work surfaces in the right front corner to accommodate the drain valve Installing the Purifier on the Labconco Base Stand Labconco offers Base Stands as an option to support the Purifier which are to be purchased separately The stands are adjustable from a working work surface height of 30 inches 76 2 cm to 36 inches 91 4 cm in 1 inch 2 5 cm increments The base stand for each Purifier model is listed in Table 3 1 below Table 3 1 Purifier Model Base Stand Model 36210 xx 37304 00 36214 xx 37306 00 For further information regarding Purifier Base Stands or other accessories please contact Labconco s Customer Service Department at 1 800 821 5525 or 816 333 8811 weekdays between the hours of 7 00 a m and 6 00 p m
46. he HEPA filter and or damage the filter s adhesive Gases under high pressure should not be used in the Purifier cabinet as they may disrupt the airflow patterns of the cabinet HEPA FILTERS ARE ONLY EFFECTIVE FOR THE ENTRAPMENT OF PARTICULATE MATTER MANIPULATIONS WHICH GENERATE GASES OR VAPORS LE TOXIC CHEMICALS OR RADIONUCLIDES MUST BE EVALUATED CAREFULLY FROM THE STANDPOINT OF BUILDUP TO DANGEROUS LEVELS THE DECONTAMINATION OF THE CABINET AND COMPLIANCE WITH APPLICABLE REGULATIONS The surface of the HEPA filters are 1 fragile and should not be touched Care must be taken to avoid puncturing either HEPA filter during installation or normal operation If you suspect that a HEPA filter has been damaged DO NOT use the cabinet contact a local certification agency or Labconco at 800 821 5525 or 816 333 8811 for recertification information THE HEPA FILTERS IN THE PURIFIER CABINET WILL GRADUALLY ACCUMULATE AIRBORNE PARTICULATE MATTER FROM THE ROOM AND FROM WORK PERFORMED IN THE CABINET THE RATE OF ACCUMULATION WILL DEPEND UPON THE CLEANLINESS OF THE ROOM AIR THE AMOUNT OF TIME THE CABINET IS OPERATING AND THE NATURE OF WORK BEING DONE IN THE CABINET IN Chapter 4 Theory Of Operation And Safety Precautions TYPICAL INSTALLATIONS AND USAGE THE HEPA FILTERS WILL LAST APPROXIMATELY FIVE YEARS OR MORE BEFORE REQUIRING REPLACEMENT Proper operation of the cabinet depends largely upon the cabi
47. hermal Protection 100 Environmental Conditions Indoor use only Maximum altitude 6562 feet 2000 meters Ambient temperature range 41 to 104 F 5 to 40 C e Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C e Main supply voltage fluctuations not to exceed 10 of the nominal voltage e Transient overvoltages according to Installation Categories II Overvoltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed e Used in an environment of Pollution degrees 2 i e where normally only non conductive atmospheres are present Occasionally however a temporary conductivity caused by condensation must be expected in accordance with IEC 664 Appendix Biosafety Cabinet Specifications C 1 SLHSIT 834078 ASNPOY 03395 BOLINON m m EEG QuvOG Q31NBjd AJIA 39lA33S 430 838038 035079 HSYS ATWO 398343J38 304 V2d 310N etit SSINYYH N d N d lSYTWB 1N395380m14 100 YOLON 1 11 WMA 001072 N d ZI 34D 9 TVMA331 G ONY STWNmH3I N33413B 33134 193NN09 39V110A VOLON YOLINOM OL 310N 09 ASM LE YAAN 0 9NIMHIM ONTO 3ounos DNI RE N NOJO D
48. holes in the bottom flange of the Purifier with its matching hole in the base stand 6 Insert the 5 16 inch mounting bolt through the hole and secure it with a flatwasher lockwasher and acorn nut 7 Repeat steps 5 and 6 for the remaining bolts 8 Tighten all four bolts securely Chapter 3 Getting Started Exhaust System Connections The Purifier Delta Series Total Exhaust Biosafety Cabinets are a Type B2 cabinet meaning they direct their HEPA filtered exhaust air out of the laboratory A The Delta Total Exhaust Biosafety Cabinet is designed to be connected to e J an appropriate exhaust system Do not attempt to operate it when it is not connected to an appropriate exhaust system THE EXHAUST CONNECTION IS A SEALED EXHAUST SYSTEM FROM THE TOP OF THE CABINET TO THE REMOTE BLOWER THE EXHAUST SYSTEMS SHOULD BE DEDICATED TO A SINGLE CABINET THE CABINET IS EQUIPPED WITH AN EXHAUST FLOW ALARM SUCH THAT AN ALARM SOUNDS AND THE CABINET BLOWER SHUTS OFF IN THE EVENT OF INSUFFICIENT EXHAUST FLOW If your research involves the use of toxic compounds or volatile materials contact your facility s safety officer or Labconco to ensure that your Purifier and its exhaust system are compatible with the materials you will be working with Exhaust System Requirements The cabinet exhaust stack is sized to accept 10 inch PVC ductwork The exhaust system s blower should be sized to handle the exhaus
49. ic interlock that must be released to reset the motor voltage 1 Remove the access panel located on the front of the electronics module by removing the right hand Phillips screw and loosening the left hand screw as shown in Figure 6 5 Pivot the panel out of the way 2 Locate the voltage test points on the electrical panel The test points are identified by Figure 6 6 shown above 3 Using a voltmeter and probes measure the motor voltage by connecting the voltmeter probes to the appropriate points 4 Shut the Purifier off 5 While holding down the alarm silence button turn the System Power switch on 6 The blower will start and the audio and visual alarm will operate in an alternating mode The blower speed can now be adjusted 7 The blower speed can be increased by pressing the button on the right pressing the button on the left decreases the blower speed as shown in Figure 6 5 8 Once the proper blower speed has been established by inflow and downflow testing the new blower voltage can be programmed into the speed controller by depressing the Alarm Silence button again The audio alarm will sound a single long tone and then shut off indicating the new voltage is properly programmed Chapter 6 Maintaining Your Purifier IF YOU WISH TO CANCEL THE NEW BLOWER VOLTAGE SETTING SIMPLY TURN THE SYSTEM POWER SWITCH OFF BEFORE DEPRESSING THE ALARM SILENCE BUTTON THIS WILL CANCEL THE NEW SETT
50. included Chapter 7 Modifying Your Biosafety Cabinet describes how to install the optional equipment on the cabinet Chapter 8 Troubleshooting contains a table of problems you may encounter while using your biosafety cabinet including the probable causes of the problems and suggested corrective actions Appendix Purifier Components contains labeled diagrams of all of the components of the biosafety cabinets Appendix B Biosafety Cabinet Dimensions contains comprehensive diagrams showing all of the dimensions for the 4 and 6 foot models of the biosafety cabinet Appendix C Biosafety Cabinet Specifications contains the electrical requirements for the biosafety cabinet Wiring diagrams for both the 115V and 230V units are also included Appendix D Purifier Accessories lists the part number and descriptions of all of the accessories available for your biosafety cabinet Appendix E Quick Chart for the Delta Purifiers provides useful operating specifications Appendix References lists the various resources available that deal with biosafety Typographical Conventions Recognizing the following typographical conventions will help you understand and use this manual Book chapter and section titles are shown in italic type e g Chapter 3 Getting Started Steps required to perform a task are presented in a numbered format Comments located in the margins provide suggestions reminders and references Cr
51. inet and forces it through the supply HEPA filter This filtered air flows down through the work area All of the downflow air plus the inflow volume are then pulled through the exhaust HEPA filter and out of the laboratory 35 Chapter 4 Theory Of Operation And Safety Precautions Speed Control The speed control should only be adjusted by a qualified certifier The speed control is an electronic circuit that allows the certifier to set the motor speed by adjusting its voltage The control will hold this voltage as line voltage fluctuates allowing for stable operation The Purifier speed control is rated for 25 Amps current far in excess of the motor s normal current draw to allow for greater reliability Cabinet Air Intakes Grilles Ductwork and Air Balance Controls Cabinet containment and performance are affected by the location size and pattern of the grilles at the front and rear of the work area Never block or obstruct the grilles of the Purifier The internal ductwork of the Purifier conveys the air from the work area to the exhaust HEPA filter The plenum above the supply HEPA filter of the Purifier is unique in the industry utilizing perfect manifold technology to deliver a more uniform airflow to the supply HEPA filter optimizing filter loading and operational life The volume of air exhausted by the Purifier is controlled by an exhaust damper system Chapter 4 Theory Of Opera
52. is 1 4 inch outside diameter soft metal and that the end has been deburred completely 2 Route the tubing from the rear of the cabinet ensuring that it will line up with the slot in the back of the side panel Make sure that the tube routing will not contact any electrical wires If routing tubing to the front valve position route the tubing under the sash weight track and at the bottom edge of the side panel Ensure that the tubing will not interfere with the sash weight when the sash is raised or lowered 3 Make sure that the tube fitting nut on the fitting is loose but do not remove it Look inside the fitting to make sure the tube ferrule is there 4 Push the tube into the fitting until it is properly seated The tube will go approximately 34 inch into the fitting 5 Tighten the tube fitting nut hand tight and then using a 7 16 inch wrench tighten it at least 3 4 turn more 6 Close the service valve in the Purifier and then slowly open the shutoff valve on the service valve Inspect the fitting for leakage Tighten the tube nut slightly if needed Chapter 3 Getting Started Figure 3 7 Tube Fitting Measuring the Total Exhaust Volume Measuring the total exhaust volume is covered in Chapter 6 Maintaining Your Purifier Measuring the Cabinet Inflow Volume Measuring the cabinet inflow volume is covered in Chapter 6 Maintaining Your Purifier 26 Chapter 3 Getti
53. itical biosafety information is presented in boldface type in paragraphs that are preceded by the biosafety icon Failure to comply with the information following a biosafety icon may result in illness or death Chapter 1 Introduction 9 Chapter 1 Introduction B e Critical information is presented in boldface type in paragraphs that are preceded by the exclamation icon Failure to comply with the information following an exclamation icon may result in injury to the user or permanent damage to your biosafety cabinet e Critical information is presented in boldface type in paragraphs that are preceded by the wrench icon These operations should only be performed by a trained certifier or contractor Failure to comply with the information following a wrench icon may result in injury to the user or permanent damage to your biosafety cabinet Important information is presented in capitalized type in paragraphs that are preceded by the pointer icon It is imperative that the information contained in these paragraphs be thoroughly read and understood by the user e Information that is specific to a particular model of Biosafety Cabinet is preceded by a number icon The 4 icon indicates the text is specific to the 4 foot model The 6 icon indicates the text is specific to the 6 foot model Your Next Step If your Purifier needs to be installed proceed to Chapter 2 Prerequisites to ensure your installation si
54. its setpoint If the alarm does not sound at the setpoint repeat steps 2 and 3 until proper operation is obtained Chapter 6 Maintaining Your Purifier 4 Open the exhaust damper until the exhaust volume is at the normal exhaust volume as indicated in Table 6 1 5 With the exhaust volume set the downflow can be established as described in Chapter 6 Maintaining Your Purifier Figure 6 3 Increase Setting Electronics Module Access The electronics module assembly should be serviced by a qualified certification technician The electronics module houses the speed control the lamp ballasts the circuit breakers as well as the motor capacitor 1 Unplug the cabinet 2 Remove the two 7 16 acorn nuts that secure the electronics module to the top of the cabinet shown in Figure 6 4 3 Pivot the module onto its back surface to access the electronic components within 69 Chapter 6 Maintaining Your Purifier Figure 6 4 Remove these two acorn nuts Then swing P the bottom of 22 the panel out and lift it straight up Measuring Line and Motor Voltage 70 Line and motor voltage contact points are high voltage connections Use caution when measuring either voltage A VOLTAGE CONNECTOR CAN BE FABRICATED FROM COMMERCIALLY AVAILABLE ELECTRONICS HARDWARE OR ONE IS AVAILABLE TO QUALIFIED CERTIFIERS FROM LABCONCO CORPORATION CONTACT LABCONC
55. l or a suitable disinfectant surface disinfect the inside of the cabinet and the work surface 59 Chapter 6 Maintaining Your Purifier e Using an appropriate glass cleaner clean the sash and the surface of the UV lamp if so equipped e Operate the cabinet blower noting the pressure reading in an operational log Monthly or more often as required e Using a damp cloth clean the exterior surfaces of the cabinet particularly the front and top of the cabinet to remove any accumulated dust e Disinfect and lift the work surface Surface disinfect the lower plenum with a solution of 70 ethanol or a suitable disinfectant Check the towel catch for retained materials e Check all service valves if so equipped for proper operation e All weekly activities Annually e Have the cabinet recertified by a qualified certification technician e Replace the UV lamp if so equipped e All monthly activities Biannually e Replace the fluorescent lamp Service Operations Work Surface Removal The work surface of the cabinet must e be thoroughly decontaminated before removing it 1 Loosen and remove the thumbscrews located at the front of the work surface as shown in Figure 6 1 2 Only loosen the thumbscrews located at the rear of the work surface 3 Lift the front edge of the work surface up and pull it out of the cabinet work area Chapter 6 Maintaining Your Purifier Towe
56. l Catch Removal Although not normally required the towel catch can be removed for cleaning inspection etc The work surface of the cabinet and 072 the towel catch must be thoroughly decontaminated before removing either 1 Remove the work surface as described above 2 Loosen and remove the thumbscrews located at the rear of the work surface 3 Surface decontaminate the towel catch before removing it Figure 6 1 Loosen and remove Only loosen these screws these screws 61 Chapter 6 Maintaining Your Purifier Front Panel Removal 1 2 Locate and remove the two Phillips screws that secure the front panel as shown in Figure 6 2 Swing the bottom of the dress panel out to clear the fluorescent light and then lift the front dress panel straight up and away from the cabinet Figure 6 2 Remove these two screws the bottom of the panel out and ift it straight up Changing the Fluorescent Lamp Unplug the cabinet Remove the front dress panel as noted in Figure 6 2 Remove the fluorescent lamp by rotating it 90 degrees and pulling it straight up and out of its sockets Remove the fluorescent lamp by pushing it to the left and swinging it out of the right lamp socket Install the new lamp by reversing the removal procedure 62 C Chapter 6 Maintaining Your Purifier hanging the Optional UV Lamp FOR
57. ly load the materials required for the procedure Do not overload the cabinet Do not obstruct the front side or rear return air grilles Large objects should not be placed close together Slowly close the sash until the TPI is engaged correctly After loading the cabinet wait two to three minutes to purge airborne contaminants from the work area Work Techniques Keep all materials at least 4 inches inside of the sash and perform all contaminated operations as far to the rear of the work area as possible Segregate all clean and contaminated materials in the work area Arrange materials to minimize the movement of contaminated materials into clean areas Keep all discarded contaminated material to the rear of the work area Avoid moving materials or the operator s hands and arms through the front access opening during use Avoid the use of an open flame Use proper aseptic technique Avoid using techniques or procedures that disrupt the airflow patterns of the cabinet If there is a spill or splatter during use all objects in the cabinet should be surface decontaminated before removal Thoroughly disinfect the working area of the cabinet WHILE IT IS STILL IN OPERATION This will prevent the release of contaminants from the cabinet Chapter 5 Using Your Purifier Final Purging e Upon completion of work the cabinet should be allowed to operate for two to three minutes undisturbed to purge airborne cont
58. model Purifier the sash height should be adjusted and measured such that a vertical line from the lowest surface of the sash handle to the front grille should equal the following height 66 Chapter 6 Maintaining Your Purifier Table 6 3 Purifier Models Sash height opening 36210 2 97 in 75 mm 36210 36214 1 99 in 51 mm 2 Start the Purifier and let it operate for at least 5 minutes 3 Establish the necessary correction factor to the thermoanemometer to ensure compliance with its performance in a calibrated wind tunnel This data should be available from the calibrator of the thermoanemometer 4 Calculate the manufacturer s recommended correction factor s to correct for local conditions of temperature humidity barometric pressure altitude etc 5 Measure a number of test points 4 inches from each side wall and at intervals of the following distance for each of these models Table 6 4 Model Distance Between each Total Number of Numbers Test Point inches Test Points 36210 36214 4 03 17 6 Take a series of inflow velocities by placing the sensor of the thermoanemometer at the test points in the center of the opening leaning the body of the probe against the front of the grille 7 Apply the wind tunnel correction and local condition correction factors to the thermoanemometer readings 8 Multiply the resulting velocities by the following Purifier correction factor to det
59. net s location and the operator s work habits Consult the Installation and Normal Operation sections of this manual for further details AVOID DIRECT EXPOSURE OF PLASTIC OR COATED MATERIALS TO ULTRAVIOLET UV RADIATION NEVER BYPASS THE SAFETY INTERLOCK OR ATTEMPT TO WORK IN THE PURIFIER CABINET WHEN THE UV LIGHT IS ON WHEN SURFACE DISINFECTING THE PURIFIER AVOID SPLASHING THE DISINFECTING SOLUTION ON SKIN OR CLOTHING ENSURE ADEQUATE VENTILATION CAREFULLY FOLLOW THE MANUFACTURER S SAFETY INSTRUCTIONS WHEN HANDLING DISINFECTANTS AND ALWAYS DISPOSE OF DISINFECTING SOLUTIONS IN ACCORDANCE WITH LOCAL AND NATIONAL LAWS DO NOT ALLOW DISINFECTANTS WITH HIGH CONCENTRATIONS OF FREE CHLORINE TO CONTACT THE STAINLESS STEEL COMPONENTS OF THE PURIFIER FOR A LONG PERIOD OF TIME FREE CHLORINE Chapter 4 Theory Of Operation And Safety Precautions D WILL CORRODE STAINLESS STEEL AFTER EXTENDED CONTACT The electrical receptacle cover because of its construction may be difficult to surface decontaminate In the event of gross contamination the cover should be removed sterilized and or decontaminated as required and discarded The receptacle cover should then be replaced with the repair part listed in Appendix A Purifier Components Biohazard cabinets should be decontaminated using formaldehyde gas for any of the following reasons Maintenance work requiring entry into contaminated areas B
60. ng Started Calibration of the Face Velocity Alarm Calibration of the face velocity alarm is covered in Chapter 6 Maintaining Your Purifier Drain Valve Installation In order to prevent damage during shipping the drain valve assembly has not been installed in the unit If desired the valve should be installed after the unit is in its final location To install the valve assembly follow these steps The work surface is heavy Use mI caution when handling it The six foot work surface is a large part Use two people and proper lifting technique when removing it from the cabinet 1 Lift the work surface out of the Purifier by removing the thumbscrews at the front of the work surface and loosening those at the rear of the pan 2 Using a putty knife carefully remove and discard the stainless steel cover that is sealed over the drain mounting holes Scrape any remaining sealant that is around the holes 3 Apply a light coating of the silicone sealant to the mounting surface of the drain assembly Attach the drain assembly as shown in Figure 3 9 Wipe off any excess sealant from the cabinet bottom Ensure that the center drain hole is unobstructed 4 Make sure the drain valve is in the closed position 5 Reinstall the work surface 6 Allow the silicone sealant to cure for at least eight hours before exposing it to liquid Chapter 3 Getting Started 28 Figure 3 9 Apply a light coating of
61. olume Measuring the Cabinet Inflow Volume Calibration of the Face Velocity Alarm Drain Valve Installation Initial Certification Your Next Step 13 15 15 19 20 23 23 24 26 26 27 27 28 29 CHAPTER 4 THEORY OF OPERATION AND SAFETY PRECAUTIONS HEPA Filters Laminar Airflow Directional Airflow Motor Blower Speed Control Cabinet Air Intakes Grilles Ductwork amp Air Balance Controls Safety Precautions Your Next Step CHAPTER 5 USING YOUR PURIFIER Operating the Sliding Sash If The Alarm Activates Resetting the Alarm Working in Your Purifier Safety and Comfort Arranging Your Work Area Your Next Step CHAPTER 6 MAINTAINING YOUR PURIFIER Routine Maintenance Schedule Service Operations Downflow Velocities Downflow Velocity Grid Patterns Air Volumes Defined Measuring the Total Exhaust Volume Exhaust Control System Adjustment Measuring the Cabinet Inflow Volume Primary Method Inflow Velocity Calculation Secondary Method Calibration of the Face Velocity Alarm Electronics Module Access Measuring Line and Motor Voltage Speed Control Adjustment Use of the Access Tube for DOP Testing HEPA Filter Replacement Motor Blower Maintenance amp Replacement Diffuser Removal Storage Decontamination Your Next Step 31 32 33 34 35 36 36 37 41 43 43 46 46 47 49 53 57 59 59 60 63 64 64 64 65 66 66 68 69 70 72 73 74 78 79 80 80 81 CHAPTER 7 MODIFYING YOUR BIOSAFE
62. ou begin working in the cabinet Even if you are an experienced biosafety cabinet user please review Chapter 5 Using Your Purifier it describes your Purifier s features so that you can use the cabinet efficiently Chapter 1 Introduction About This Manual This manual is designed to help you learn how to install use and maintain your biosafety cabinet Instructions for installing optional equipment on your cabinet are also included Chapter 1 Introduction provides a brief overview of the biosafety cabinet explains the organization of the manual and defines the typographical conventions used in the manual Chapter 2 Prerequisites explains what you need to do to prepare your site before you install your biosafety cabinet Electrical and service requirements are discussed Chapter 3 Getting Started contains the information you need to properly unpack inspect install and certify your biosafety cabinet Chapter 4 Theory Of Operation And Safety Precautions explains how the Purifier operates and the appropriate precautions you should take when using the cabinet Chapter 5 Using Your Purifier discusses the basic operation of your cabinet Information on how to prepare use and shut down your Purifier are included Chapter 6 Maintaining Your Purifier explains how to perform routine maintenance on your biosafety cabinet Information on how to safely disinfect the interior of your cabinet and replace the lamps are
63. quirements for your installation site the space requirements for your installation site Refer to Appendix C Biosafety Cabinet Specifications for complete biosafety cabinet electrical and environmental conditions specifications and requirements Chapter 2 Prerequisites Location Requirements The Purifier should be located away from traffic patterns doors fans ventilation registers fume hoods and any other air handling device that could disrupt its airflow patterns All windows in the room should be closed Figure 2 1 shows the optimum location for the Purifier DO NOT locate the cabinet on a cart dolly or mobile bench ALL Purifier installations must be permanent and stationary Figure 2 1 VENTILATION REGISTER BLOCKED WHEN FACING PURIFIER L AIRFLOW INTO ROOM ALTERNATE PURIFIER LOCATION PURIFIER LOCATION CAS EWO R K Exhaust Requirements BEFORE DECIDING ON A gt LOCATION EXAMINE IT CAREFULLY TO ENSURE THATIT IS COMPATIBLE WITH THE CABINET S EXHAUST DUCT THE AREA DIRECTLY ABOVE THE CABINET S EXHAUST PORT SHOULD BE CLEAR OF STRUCTURAL ELEMENTS WATER AND UTILITY LINES OR OTHER FIXED OBSTRUCTIONS THERE SHOULD BE ENOUGH CLEARANCE TO ALLOW FOR THE PASSAGE OF A 10 PVC DUCT AVOID CABINET LOCATIONS THAT REQUIRE EITHER AN ELBOW DIRECTLY ON TOP OF THE CABINET S EXHAUST CONNECTION OR AN EXCE
64. rective s 73 23 EEC 89 336 EEC Standard s to which conformity is declared EN61010 EN55014 EN55104 Manufacturer s Name Labconco Corporation Manufacturer s Address 8811 Prospect Avenue Kansas City MO 64132 USA Importer s Name See Shipping Customs Documents Importer s Address See Shipping Customs Documents for your equipment Type of Equipment Laboratory Equipment Model No Purifier Delta Series Biological Safety Cabinets 36205 24 3 Ft Wide 36209 24 4 Ft Wide 36213 24 6 Ft Wide Serial No Various See Individual Declaration Year of Manufacture 2000 and Subsequent I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s See individual Declaration of Conformity which will be signed by the importer for your country Signature Full Name Position An individual version of this declaration is included with your shipping customs documentation Labconco P N 36960 20 REV ECO B296 115 For more information please contact us E mail sales expotechusa com Website www ExpotechUSA com
65. rews and locking nuts secure the UV ballast to the enclosure as shown in Figure 7 1 Wire the UV ballast into the wiring harness following the wiring diagram located in Figure 7 1 Raise the sash and install the UV lamp by rotating the lamp pins into the sockets Lower the sash to fully closed position plug in the unit and turn the system power switch to the UV position The UV lamp will illuminate Chapter 7 Modifying Your Biosafety Cabinet Figure 7 1 3 ATTACH TO STUD ON CABINET TOP 85 Chapter 7 Modifying Your Biosafety Cabinet 6 6 foot units only require the installation of the UV lamp The lamp is part number 12713 00 lt gt Completely surface decontaminate all interior work surfaces of the Purifier 9 before beginning the installation of the UV lamp To install the UV lamp kit do the following 1 Unplug the unit and raise the sash to the full open position 2 Install the UV lamp by pushing it into the left socket and compress the socket swinging it into the right lamp socket 3 Lower the sash to fully closed position plug in the unit and turn the system power switch to the UV position The UV lamp will illuminate Installing Additional Service Fixtures Kc Completely surface decontaminate all ev interior work surfaces of the Purifier 02 before beginning the installation of the service valve Additional service fixtures can be installed in any of th
66. rials in this area where airflow is critical The air foil is epoxy coated stainless steel and may be factory installed or retrofitted on site Contact Labconco at 800 821 5525 816 333 8811 or labconco labconco com for ordering information Base Stands Epoxy coated steel adjust in 1 inch increments to provide a working height from 30 to 38 inches 29 14 inches deep x 27 2 to 34 inches high NSF tested and approved for use with the Purifier ADA compliant Catalog For use with Width Shipping Weight 37304 00 4 foot Purifier Delta Series 50 1 2 128 82 lbs 37 kg 37306 00 6 foot Purifier Delta Series 74 1 2 189 cm 100 lbs 45 kg 107 D Purifier Accessories 108 Service Valve Kit 37475 00 Includes serrated hose tip valve with quarter turn handle hardware and instructions for plumbing to services mounts on left or right side interior All cabinets are factory prepared to accept up to 4 fixtures Shipping weight 4 lbs 2 kg Ultraviolet Lamp Kits Includes 254 nm UV lamp Kit 37450 00 also includes a 115 volt ballast Catalog For use with Shipping Weight 37450 00 4 foot Purifier Delta Series 5 lbs 2 3 kg 37450 01 6 foot Purifier Delta Series 3 Ibs 1 4 kg IV Bar Kits Bar supports intravenous solution bottles and bags Kits include IV bar mounting hardware and four hangers Ca
67. s may cause unnecessary strain Shoulders and Elbows Adjust your chair height or Purifier height so that your shoulders are relaxed and your elbows hang comfortably at your sides Relax Remember to relax particularly in areas where muscle tension often builds such as your shoulders Eyes Working at your Purifier for long periods can be a visually demanding task and may cause your eyes to become irritated and fatigued Therefore you should give special attention to vision care including the following recommendations Chapter 5 Using Your Purifier Resting Your Eyes Give your eyes frequent breaks Periodically look away from the work area and focus at a distant point This may also be a good time to stretch breathe deeply and relax Cleaning Your Sash and Glasses Keep your sash and glasses clean Having Your Eyes Examined To be sure that your vision is adequately corrected have your eyes examined regularly by a vision care specialist If you wear bifocals or trifocals you may find monofocal glasses more comfortable for cabinet use Eye comfort While working in your Purifier and also while resting your eyes remember to blink This helps keep your eyes naturally protected and lubricated and helps prevent dryness a common source of discomfort GIVE YOUR EYES FREQUENT RESTS BY FOCUSING THEM ON A DISTANT POINT Arranging Your Work Area Eliminating Glare and Reflections on the Sash Take th
68. s shown in Figure 3 2 3 Remove and discard the four screws that secure the unit to the skid as shown in Figure 3 1 4 Remove the two sash retaining screws and brackets from the bottom edge of the sash Discard the screws and brackets Figure 3 1 Remove and discard the cardboard corner posts and packing on top Remove the side panels as shown in Fig 3 2 I Remove and discard these screws two on each side Remove and discard these two screws and brackets Figure 3 2 Remove these three N Acorn nuts and washers Remove these Phillips screws 5 Examine the sash cables at both upper front corners of the Purifier as shown in Figure 3 3 The cables should rest securely in the pulley Hu Ensure that both sash cables are properly oriented before proceeding Releasing the sash weights with the cables improperly positioned can damage the cables pulleys or its axle 6 Locate the weight restraining bolt in each weight channel It is a 1 4 inch diameter bolt that runs from the front through to the rear of the channel Its location in the channel will vary from model to model Remove each bolt while supporting the sash cable ensuring that it is seated in the pulley Save the bolts for future use if the unit is to be transported 7 Install the two sash stop screws and spacers on the front of the cabinet as shown in Fig
69. sites 12 GETTING STARTED Now that the site for your biosafety cabinet is properly prepared you are ready to unpack inspect install and certify your unit Read this chapter to learn how to unpack and move your Purifier set up the cabinet connect to an exhaust system connect the electrical supply source connect the service lines arrange certification of your Purifier Depending upon which model you are installing you may need common plumbing and electrical installation tools in addition to two 1 2 wrenches a 7 16 wrench a flat blade screwdriver a Phillips screwdriver and a carpenter level to complete the instructions in the chapter The Purifier models weigh between 600 800 Ibs 182 318 kg The carton allows for lifting with a mechanical lift truck or floor jack If you must lift the Purifier manually use at least six 6 persons and follow safe lifting guidelines Chapter 3 Getting Started The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen 15 days of delivery 14 Unpacking Your Biosafety Cabinet Carefully unpack your Purifier and inspect it for damage that may have occurred in transit If your unit is damaged notify the delivery carrier immediately and retain the entire shipment intact for inspection by the catrier DO NOT RETURN GOODS lt gt WITHOUT THE PRIOR AUTHORIZATI
70. t and into the exhaust system The inflow is the amount of air that flows through the front access opening Measuring the Total Exhaust Volume Measure the airflow in the exhaust duct in accordance with American Society of Heating Refrigerating and Air Conditioning Engineers ASHRAE recommended procedures It should correspond to the airflow values shown in Table 6 1 Chapter 6 Maintaining Your Purifier Table 6 1 Purifier Airflow Data Model Average Inflow Work Opening Exhaust volume Normal Exhaust Alarm Setpoint Number Velocity FPM Area sq ft of Cabinet Volume of Volume CFM Cabinet CFM 36210 xx 105 2 69 743 771 772 730 36214 xx 105 4 03 1111 1151 1187 1091 Using a direct read instrument sealed to the sash opening with the air intake blocked Measured using a pitot traverse of the exhaust duct as per ASHRAE recommendations Balancing of the exhaust flow is accomplished by adjusting the cabinet s exhaust damper Changing the damper setting or the speed control will affect the inflow and downflow values Both must be checked after any adjustment Downflow and inflow values and measurement procedures are described fully in Table 3 6 and Chapter 6 Maintaining Your Purifier Exhaust Control System Adjustment l Adjusting the exhaust control system will have an effect on the air velocities and the efficiency of the cabinet The exhaust
71. t volume of each cabinet as shown in Table 6 1 with a static pressure equal to or Chapter 3 Getting Started greater than the system pressure plus 1 2 inches of water In order to get the maximum life out of the cabinet s exhaust filter the system needs to be able to pull a vacuum of 3 2 inches of water at the cabinet s rated flow volume HN The exhaust system should be fitted with a backdraft damper to prevent the reversing of airflow in the system LABCONCO MANUFACTURES AN EXHAUST BLOWER WITH INTEGRAL BACKDRAFT DAMPER PART NUMBER 36635 THAT IS SUITABLE FOR MOST SINGLE CABINET INSTALLATIONS THE REMOTE BLOWER IS DISCUSSED FURTHER IN APPENDIX D Balancing of airflows in the cabinet is accomplished by adjustment of the cabinet s exhaust control system and speed control The exhaust system should be considered a separate system distinct from the Purifier Changing the damper setting or the speed control will affect the inflow and downflow values Both must be checked after any adjustment Downflow and inflow values and measurement procedures for the Purifier are described fully in Chapter 6 Maintaining Your Purifier Connecting The Purifier to Service Lines The service lines if any should be connected to the tube fitting s on the outside of the liner wall as shown in Figure 3 7 To install the tubing follow these steps Chapter 3 Getting Started 1 Ensure that the tubing
72. talog For use with Shipping Weight 37455 01 4 foot Purifier Delta Series 4 Ibs 1 8 kg 37455 02 6 foot Purifier Delta Series 6 lbs 2 7 kg Appendix D Purifier Accessories Remote Blower 36635 00 24 5 16 wide x 21 1 2 deep x 22 3 8 high Motor blower shall be wired for operation on 230 460 volts 3 phase 60 Hz 5 8 2 9 amps AC The 2 hp motor shall be TEFC type totally enclosed fan cooled Blower shall be belt driven with an adjustable sheave Belt shall have a self adjusting gravity tightener Pillow block bearings shall be factory lubricated and sealed Impeller shall be constructed of phenolic coated steel Blower shall have a dry powder epoxy coated base and weathercover with vent holes An integral damper assembly shall prevent backdrafts from flowing down through the ductwork disturbing the airflow within the cabinet Blower transition adapter inlet shall be 12 1 4 OD and shall be sized to accept 12 nominal diameter PVC duct Blower transition adapter outlet shall be sized to accept 12 nominal diameter PVC duct Shipping weight 100 Ibs 45 kg Ergonomic Chair with Armrests 37440 00 Designed to Biosafety Guidelines Lab chair has 6 way articulating seat and back control for personalized adjustment Pneumatic mechanism adjusts seat height from 18 1 4 to 25 3 4 Five leg black reinforced composite base rests on 2 ball bearing casters Aluminum support ring Removable arm rests
73. te meets all of the requirements Then go to Chapter 3 Getting Started for instructions on how to install your biosafety cabinet and make all of the necessary connections If you would like to review how biosafety cabinets operate go to Chapter 4 Theory Of Operation And Safety Precautions For information on the operational characteristics of your biosafety cabinet go to Chapter 5 Using Your Purifier Chapter 1 Introduction If your biosafety cabinet is installed and you need to perform routine maintenance on the cabinet proceed to Chapter 6 Maintaining Your Purifier For information on making modifications to the configuration of your unit go to Chapter 7 Modifying Your Biosafety Cabinet Refer to Chapter 8 Troubleshooting if you are experiencing problems with your Purifier Chapter 1 Introduction 2 PREREQUISITES Before you install your biosafety cabinet you need to prepare your site for installation Carefully examine the location where you intend to install your cabinet You must be certain that the area is level and of solid construction In addition a dedicated source of electrical power must be located near the installation site Carefully read this chapter to learn e the location requirements for your installation site e the exhaust requirements for your installation site e the electrical power requirements for your installation site e the service line re
74. tion And Safety Precautions Safety Precautions The Purifier Biohazard Cabinet should be certified by a qualified certification technician before its initial use The cabinet should be recertified whenever it is relocated serviced or at least annually thereafter Some components of the Purifier Biohazard Cabinet should only be serviced by a qualified certification technician Some internal components of the Purifier may become contaminated during operation of the unit Only experienced personnel competent in the decontamination procedure should decontaminate the cabinet before servicing contaminated components If you have any questions regarding certification agencies or need assistance in locating one contact Labconco s Product Service Department at 800 522 7658 or 816 333 8811 Ensure that the unit is connected to electrical service in accordance with local and national electrical codes Failure to do so may create a fire or electrical hazard Do not remove or service any electrical components without first disconnecting the Purifier from electrical service Avoid the use of flammable gases or solvent in the Purifier Care must be taken to ensure against the concentration of flammable or explosive gases or vapors An open flame should NOT be used in the Purifier Open flames may disrupt the airflow patterns in the cabinet 37 Chapter 4 Theory Of Operation And Safety Precautions burn t
75. u can make to reduce or eliminate the sources of stress BREATHE FRESH AIR DEEPLY AND S REGULARLY THE INTENSE MENTAL CONCENTRATION THAT MAY ACCOMPANY WORK IN BIOSAFETY CABINET MAY TEND TO CAUSE BREATH HOLDING OR SHALLOW BREATHING Self Check To increase your comfort and reduce potential safety risks use this checklist to help you evaluate your work posture and habits Chapter 5 Using Your Purifier Chapter 5 Using Your Purifier Seated Position e Have you found a range of seated postures that are most comfortable for you e Are you changing postures within your comfort zone throughout the day especially in the afternoon Are your feet firmly planted Are the undersides of your thighs near your knees free of pressure e Are the backs of your lower legs free of pressure Is there sufficient space under your cabinet for your knees and legs e s your lower back supported Shoulders Arms Wrists and Hands e Are your shoulders relaxed e Are your hands wrists and forearms aligned in a straight natural position e Are your elbows in a relaxed position near your body e Are items you use frequently easy to reach e Do you rest your eyes frequently by focusing on a distant point e Do you get your eyes examined regularly by a vision care specialist Do you blink enough e If you wear bifocals or trifocals do you avoid tilting your head back to see your work Working Style e Are you tr
76. ure 3 4 Chapter 3 Getting Started Chapter 3 Getting Started 8 Raise and lower the sash to check it for proper operation The sash should move smoothly If the sash binds or requires excessive force to operate examine the cables and pulley for proper operation 9 Examine the right weight track as shown in Figure 3 3 When the sash is completely closed and resting against the sash stop the top surface of the weight should be 1 5 8 41 mm below the top of the weight channel If it is not adjust the weight channel by loosening the six nuts that secure the channel to the side wall of the cabinet Slide the weight channel up or down until the dimension is proper Retighten all six nuts to secure the channel Figure 3 3 Ensure the top of the weight is 1 5 8 41 mm below the top of the weight track when the sash is completely closed and resting on the sash stops Loosen these six nuts three on each side of the weight track to adjust the position of the weight track Remove these weight restraining bolts from each weight channel ES AN f AN A Figure 3 4 N NN N N NN N X N W NN Install the two sash stop a screws and spacers on the front of the cabinet k Installing the Purifier On An Existing Work Surface The Purifier is very top heavy Use caution when lifting or movin

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