Mai Tai™ - AlphaMetrix
Contents
1. Your oven is now calibrated to provide accurate temperatures between ambient 5 to 99 C G Loading and Removing Rotators ik Place end of shaft on left bearing Insert shaft ball with alignment pin into right bearing All rotators are loaded and removed using the same two step process When loading first insert the end of the shaft that ends in a ball joint with alignment pin into the slotted bearing on the right wall of the chamber The left end of the shaft is then placed into the bearing on the left side of the chamber Reverse this procedure when removing the rotator Version 1 0 April 2008 8 408 733 7337 techserv scigene com Sc G e NM Mai Tai Hybridization System USER MANUAL IV USING MAI TAI HYBRIDIZATION CASSETTES 1 With Mai Tai Rotator installed pre heat the hybridization oven to the desired incubation temperature 2 Place the base plate on a flat surface such as a laboratory bench 3 Place a gasket slide into each chamber in the cassette with the Agilent label facing up and aligned with the barcode side of the chamber as shown in Figure 2 When placing the gasket slide make sure the slide does not rest on the edge of the base plate Figure 3 To familiarize new users with the placement technique practice placing ordinary 1 x 3 25 mm x 75 mm glass slides into the chamber prior to running an experiment Agilent label face up Orient label with BARCODE N2. a YES FIGURE 2 FIGURE 3 4 Pipette the labeled probe into each chamber of the gasket slide using the volumes shown in the table below for each gasket slide format To prevent spilling or leaking dispense the hybridization solution to the center of the gasket well without touching the gasket with the pipette tip Figure 4 shows hybridization solution added to a 4 x gasket slide DISPENSE PROBE IN MIDDLE OF GASKET COMPARTMENT Gasket Probe Format Volume 1 x microarray 520 ul 2x microarray 260 ul FIGURE 4 5 Holding array at the ends with the array side down and ensuring that the barcode side matches with the one on the gasket slide bring the array down slowly holding it horizontal to the gasket slide Figure 5 ARRAY FACE DOWN FIGURE 5 Version 1 0 April 2008 9 408 733 7337 techserv scigene com SC l G e NM Mai Tai Hybridization System USER MANUAL A Placing the array evenly onto the gasket fex slide is crucial to prevent spilling or leakage ZN Use Agilent arrays or microarrays from other sources that are compatible with the Agilent asket slides g NO YES FIGURE 6 6 When all arrays have been placed make sure all assemblies are laid flat against the base plate See Figure 6 for examples of proper and improper placement 7 Place the cover plate over the arrays with TIGHTEN THUMBSCREWS the barcode window matching the FINGERS ONLY NO TOOLST barcodes on array See Figure 7 show
3. use or performance of the product Copyright Copyright 2008 SciGene Corporation All rights reserved SciGene is a trademark of SciGene Corporation Sunnyvale CA All other trademarks used in this manual are the property of their respective owners Version 1 0 April 2008 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL Table of Contents L SAFETY NOTICES sosicenni sini VPSUREOHUEN EIN aaeain inaani aain 3 As end SS a E E E E E EA 3 B ASUNE Sale a a E E OE E E 3 Ce VS ee E E E E EEE E 3 D LUON a E ene saesennsedessmmenes caren sens 3 E Lifting and Moving he Unibsersrsssrnernr Penne ee eee 3 Il UNPACKING AND SETTING UP YOUR OVEN ssccsssstsscssssssscssssnssssessensssesssnseceess 4 A Removing the Unit from the Carton cccccecececsceeeeeeseteteceeseaeeeaeaeeeseterseaeaeaeatans 4 b Pari POJEO eo nee ee ee ee eee ee ee 4 Ce WS CATION eeeuererzsecedtrastecren qe IUe on Ehre taconeenuneneene E E E 4 Ill USING YOUR OVED NL css n r siiERenE HskE T CREER EREEIDIME MEER VK RI UN ERU E RD E Ed 5 A Oven Components and Controls lsesesessssesssssseen enemies nennen nnns 5 B Closing and Opening the DOOL cccceceeeeeeseseeteceteeseeceeececececececeatataeseatatanatatatas 6 C Using the Temperature Controller eeeeseeeeeeennem mmm nnn nnn 6 D Seng RO Or SDOGG Lonusxvsxumexiaden MM eda Tr at URGE MN rdMU MUR M RM eH 6 E Using the
4. JOG SWIECH cec tied enata aida y a PED xen XD hoa frt Ki aD Pel d p RR oO aat 6 F Calibrating the Oven Temperature cccceceesececeeeeeeeeeteeseaeetaeaeaeaeeeetetetetenenesers 7 G Loading and Removing Rotators sesesesesesesene nennen memes sese nnns 8 IV USING MAI TAI HYBRIDIZATION CASSETTES rrr erre 9 V CLEANING AND STORAGE OF MAI TAI HYBRIDIZATION CASSETTES 11 VI MAINTAINING YOUR OVEN 71 rrr rrr nnn nnum uuu numum uuu un nuuuuuunnnuuuss 12 Pe SOC UNG cT E 12 Be Ce ndm 13 VIIL TROUBLESHOOT ING ssnssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 14 VULSPECIFICA FIONS sexi akkEGN NUR RE REK RAIN aaia aaa 14 IX CHAMBERS ROTATORS AND ACCESSORIES 11 1er rr rrrnn nnns 14 Version 1 0 April 2008 2 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL I SAFETY NOTICES A Intended Use This instrument is intended for the heating and incubation of laboratory samples This instrument should only be used according to the instructions provided in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired B Instrument Safety Before operating the instrument read the information in this se
5. jog switch is used to momentarily advance the rotator when the door is open When the jog switch is pressed the rotator will turn at the speed selected on the speed controller It will not move if the speed controller is turned off or is set below 2 RPM Version 1 0 April 2008 6 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL F Calibrating the Oven Temperature The Watlow 93 temperature controller comes calibrated by SciGene to provide accurate chamber temperatures from ambient 5 to 99 C Calibration of the controller is required only if when verifying with a NIST certified digital thermometer SciGene Cat 1051 52 0 the chamber temperature differs by more than one degree from the actual temperature shown on the display Do not use a glass thermometer To adjust the controller to achieve accurate oven temperatures ir With the oven powered on enter a temperature of 65 C on the controller and allow the temperature on the display to stabilize about 20 minutes Using the cable provided with the z digital thermometer plug one end into the blue jack found on the back panel and the other into the digital thermometer Turn ON the j thermometer Back of Oven On the temperature controller press the up and down arrows simultaneously for 3 seconds The upper display will now read 3 and is the lower display Loc De Thermometer Using the dow
6. to the unit using the switch on the front of the unit Version 1 0 April 2008 4 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL Ill USING YOUR OVEN A Oven Components and Controls e Power Switch Turns on main power to unit e Temperature Controller Used to set and observe chamber temperature e Rotator Control Turns on and controls the speed of the rotator e Jog Switch Activates rotator with door open at the speed selected e Door Latch Tightly secures the door closed with button release to open Temperature Controller Heater ON indicator Alarm indicator when configured Power indicator not in use Actual Temperature Set Temperature Scroll Key for calibration only Up Ke Down Key Power Adjustment Do NOT Use Power Switch Jog Switch Rotator Control Door Latch SciGene Push door closed and press latch lever to secure Version 1 0 April 2008 5 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL B Closing and Opening the Door To secure the door press the door against the cabinet to close and push down the top lever To open press the lower latch release button C Using the Temperature Controller The features of the Watlow 93 temperature controller on your oven are shown
7. 30 0 Rotator for 96 Affymetrix GeneChips 1077 10 0 Rotator for 48 Affymetrix GeneChips Hybridization Chambers Version 1 0 April 2008 14 408 733 7337 techserv scigene com
8. SciGene wwscigenecom Mai Tar Hybridization System USER MANUAL Cat 1075 00 1 1075 00 2 n FOR RESEARCH USE ONLY Not for Use in Diagnostic Procedures SciGene e 306 Potrero Avenue Sunnyvale CA 94085 USA e 408 733 7337 e Fax 408 733 7336 e techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL Serial Number The following serial number identifies the specific instrument you have purchased and must be referenced when requesting service A copy is affixed to the instrument Technical Service 408 733 7337 techserv scigene com Warranty SciGene warrants that the heating unit described in this manual shall be free of defects in materials and workmanship for a period of 12 months from date of delivery This warranty does not cover removable blocks or accessories In the event of a defect during the warranty period SciGene s limit of liability will be to provide replacement parts at no charge or at its sole discretion replace the product The foregoing warranty is void in the event the unit was abused or modified or used in a manner inconsistent with its intended purpose SciGene makes no other warranty expressed or implied including warranties of merchantability and fitness for a particular purpose In no event shall SciGene be liable for any direct indirect special incidental or consequential damages or for any damages resulting from loss arising out of or in connection with the sale
9. TING UP YOUR OVEN A Removing the Unit from the Carton C Position the shipping carton on the floor close to the location of intended installation Remove the foam inserts from the top of the unit Position one person at the front and one at the back of the oven Grip the recessed edges along the top of the unit and lift it straight up and out of the shipping carton taking care to keep it balanced Place it on the bench or table where it will be installed Carefully inspect the unit for damage If there is evidence of damage do not discard the shipping materials since they may be needed to return the unit Parts Provided The following items are included with the system Model 777 Microarray Oven Mai Tai Rotator Mai Tai Cassettes 2x Oven Power Cord User Manual Installation 1 Placement Place the unit on a stable level surface within a few feet of the power source Avoid locating it below a shelf where solutions are stored A spill may enter the electrical compartment through the vent holes on the top of the unit and cause damage Ensure there is adequate clearance along the front and right side of the oven to open the door completely There should be a minimum clearance of 3 inches along the top and back panels for air circulation 2 Connecting the Power Plug the power cord provided into the back of the unit and then to a properly grounded outlet Use only the power cord provided 3 Powering On Turn power ON
10. cement parts and perform these repairs B Cleaning Clean the exterior and interior surfaces using a mild detergent based spray cleaner and wipe with a soft cloth Under no circumstances should solutions be allowed to enter the electronics package through the ventilation slots on the top of the unit Do not use abrasive cleansers or scouring pads that can scratch the stainless steel Version 1 0 April 2008 13 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL VII TROUBLESHOOTING Symptom Cause Solution Rotator does not turn Rotator control is in the off Turn on rotator after closing door position Door does not shut or is Latch is improperly adjusted Adjust threaded rod difficult to open on inside of latch Thermal controller is defective Replace controller VIII SPECIFICATIONS Electrical Model 777 Oven Dimensions Z 1 0 0 0 0 0 0 0 0 Interior Chamber Exterior Net 70 Ibs 32kg Gross Performance and Controls Heat up Time 3 C per minute Digital PID single loop Actual Set Dual LED Rotation Speed 2 to 80 RPM Chamber Stainless Steel Thermocouple IX CHAMBERS ROTATORS AND ACCESSORIES Ca 23 3 A Descipion UOM 1075 10 0 Mai Tai Hybridization Cassette Holds four slides 1051 52 0 Handheld electronic digital thermometer with Type T Each thermocoupler includes NIST traceable certificate of calibration and interface cable 1077
11. ction concerning hazards and potential hazards Ensure that anyone involved with the instrument s operation is instructed in both general safety practices for laboratories and specific safety practices for the instrument C Warnings Failure to comply with the following warnings that are affixed to the product can lead to possible personal injury or death For the continued protection against risk of fire replace only with Do not use in vicinity of flammable or combustible materials fuse of the specified Fire or explosion may result type and current ratings HAZARDOUS VOLTAGE Disconnect power before servicing D Cautions Failure to comply with the following cautionary statement affixed to the product Hot surfaces may lead to possible personal injury Items inside heated in the oven should be handled with Avoid contact heat protective gloves Open all closed vessels heated in the oven with the opening pointed away from you to avoid contact with heated aerosols E Lifting and Moving the Unit The rotating oven you have purchased weighs approximately 70 Ibs 32 kg Use caution when lifting the unit to protect you and others from personal injury It is strongly recommended that two people lift the unit simultaneously to keep it balanced and to share the load Version 1 0 April 2008 3 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL UNPACKING AND SET
12. he array supplier 14 Wash and dry the microarray on the Little Dipper Processor SciGene Cat 1080 40 1 115v 1080 40 2 220v or manually using the buffer specified by the array supplier Version 1 0 April 2008 10 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL V CLEANING AND STORAGE OF MAI TAI HYBRIDIZATION CASSETTES Hybridization solution may occasionally spill and dry on to Mai Tai base and cover plates making arrays or gasket slides stick to these parts during hybridization Base plates cover plates and thumb screws need to be cleaned periodically to prevent such sticking Follow the protocol below to clean Mai Tai parts 1 Disassemble each Mai Tai cassette into base plate cover plate and thumb screws Captive thumb screws can be unscrewed from the cover plate 2 Using a lint free wipes soaked in 7096 ethanol wipe all surfaces of base plate cover plate thumb screws and thumb screw hole carefully to remove dried hybridization solution and salts Allow the clean parts to dry completely by evaporation 4 Assemble the parts back together using the thumb screws and store in a dry place UJ Version 1 0 April 2008 11 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL VI MAINTAINING YOUR OVEN A Servicing Turn the power switch to the OFF position and unplug the power cord before performing any service proced
13. in the illustration Section III A It has two LED readouts and four membrane buttons The upper readout displays the ACTUAL temperature The lower readout displays the SET chamber temperature The button with the circle icon at the lower left is used to scroll through the controller parameters when performing calibration of the controller It is not used during normal operation The up and down arrow buttons are used to adjust the desired set temperature They are also used when calibrating the oven The infinity symbol button on the lower right of the panel is for adjusting the power that is delivered to the chamber heaters It is set at 100 at the factory and should not be adjusted during normal operation To set oven temperature simply push the up and down arrows until the desired temperature is shown The unit will now adjust the heat of the chamber until the SET temperature is attained The controller is calibrated at the factory to provide an accurate chamber temperature when operated from ambient 5 to 99 C It is recommended that the temperature controller be recalibrated monthly to ensure accurate oven temperatures D Setting Rotator Speed The rotator is turned on and off and its speed of rotation controlled through the rotary switch Turn the dial clockwise from its stopping point to turn on the rotator drive system and set the speed of rotation Rotation speed is adjustable from 2 to 80 RPM E Using the Jog Switch The
14. ing b correct placement and orientation of the cover plate 8 Tighten the thumb screws all the way to a complete stop Do not use tools 9 Rotate the cassette to ensure that the bubbles move freely inside each array ALIGN WINDOWS WITH BARCODE chamber LABELS For optimal hybridization it is best to FIGURE 7 A have a single large bubble which ii moves freely around the chamber when rotated After assembly ensure that there are no stationary bubbles in any array chamber when the assembly is rotated Stationary bubbles can be released by gently tapping each corner of the assembly on a firm surface as it is rotated MAI TAI 10 Place the assembly into the Mai Tai Rotator in the ROTATOR pre heated oven Raise the spring bar and load each Mai Tai Cassette into the sleeves of Mai Tai uuu Qei BER __ SciGene Oven Rotator as shown in Figure 8 eee Model 777 When loading multiple cassettes load equal A number of cassettes on either sides of the WAITAI rotator to balance weight SERING HYBRIDIZATION CASSETTE 11 Rotate the Mai Tai Rotator at the speed and time FIGURE 8 recommended by the microarray manufacturer 12 At the completion of the incubation period remove the cassette s from the rotator and allow to cool for a few minutes Loosen thumbscrews and remove the top plate 13 One at a time remove the array gasket slide sandwich and separate them while submerged in the buffer specified by t
15. n arrow set the upper display to 0 The controller is now unlocked and available for calibration Sequentially press the circle icon button until the display shows the Actual and Set temperatures of the oven Calculate the difference in temperature shown on the Actual display of the controller and the digital thermometer For example if the controller displays 52 5 C and the digital thermometer displays 51 0 C the difference is 1 5 C Sequentially press the circle icon button until the lower display reads Cal The upper display shows the offset value between the controller and digital thermometer when the unit was last calibrated Using the up and down arrows adjust the offset value to the difference in temperatures calculated in Step 6 For example if the controller displays a temperature that is 1 5 C higher than the digital thermometer adjust the offset value to minus 1 5 Version 1 0 April 2008 7 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL 9 Sequentially press the circle icon button until the display shows the Actual and Set temperatures The Actual temperature on the display should now match the digital thermometer 10 Press the circle icon button until Loc is shown on the lower display Press the up arrow until 3 is shown in the upper display 11 Sequentially press the circle icon button until the Actual and Set temperatures are again shown on the display
16. ure l Checking and Replacing Fuses Fuses are located in a removable fuse block below the power cord receptacle on the back of the unit Unplug the cord and using a flat blade screwdriver remove the fuse block as shown in the illustration Remove the fuse holder from the block and gently remove the two fuses A blown fuse will appear dark Always replace a blown fuse with fuses matching the amperage and voltage as shown on the label below the fuse block Use small screwdriver behind Digital Thermometer Jack tab to open Fuse Block na Back of Unit Push clip up to release Fuse Holder Version 1 0 April 2008 12 408 733 7337 techserv scigene com SC G e NM Mai Tai Hybridization System USER MANUAL 2 Replacing the Temperature Controller If the actual temperature reported on the controller is erratic after calibration the temperature controller may need to be replaced Please contact SciGene Technical Services techserv scigene com for assistance in troubleshooting If needed replacement controllers are available SciGene cat ZRP600 01 46 03 3 Rotator Drive System The rotator drive system is designed to provide trouble free operation for many years and does not require routine maintenance However components of the drive system that fail due to normal wear and tear over long term usage can be easily replaced Contact SciGene s Technical Service Department techserv scigene com to obtain repla
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