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1. EEEE EEI am me Ee E JAE m 7 Se a Thermal Cycler Tel 91 11 4500 7000 Eraik ifo genelidiotech com Webste www genelixbiolech com Ta 91 1 4502 7000 Emai nfo genetidiotech com Website wwwigenetixbiotech com Thermal Cycler Manual Control In Manual Mode the Palm Cycler block can be set to hold a specified temperature for an indefinite time This function is useful for incubating reactions such as DNA digestion or ligation Simply select Manual Mode from the control menu and key in the temperature desired Manual Mode also supports a thermal gradient in which the upper and lower temperaturesareset Touchdown And Long Range Touchdown and Long Range are auto increment programming tools supported by the Palm Cycler Touchdown allows the temperature of a step to be automatically lowered by a set amount over a specified range of successive cycle repeats It is usually used as a mechanism to avoid primerdimer artifacts by gradually lowering the annealing temperature during early cycle repeats of an amplification reaction Long Range programming enables the time of a particular step to be automatically increased by a set amount over a specified range of successive cycle repeats Long Range programming is often used to provide gradually extended times for enzymatic polymerization of longer products during later cycle repeats of an amplific
2. CG Palm Cucler Gradient Thermal Cycling The new Gradient Palm Cycler combines robust gineering advanced performance and a palmtop PC interface for sup friendly flexibility Active cooled block range 4 C to 99 C 8 Peltiers and 4 platinum sensors True linear gradient capability 1 C to 24 C Intuitive Palm computer interface Easy programming and backup e Advanced cycling options Touchdown Long Range Ramp Rate Hold List etc e Advanced functions graphing interface autocalibration oligo calculator etc e Built tough to last Familiar Windows operating system Allows total flexibility in cycle programming SD memory cards for program transfer amp backup Computer supports advanced software for autocalibration primer design virtual runs etc Intuitive graphical user interface with large backlit color touch sensitive screen In built palm calendar software makes a perfect lab booking system It is an indigenous product Oligo Calculator Software Thermal Cycler 1 4502 7000 Erraitifo genetbbotech com Webster warw genetxbiatech com Ta 91 1 4502 7000 Emai info genetibiotech com Website wwwgenetixbiotech com Thermal Cycler Palmtop PC Interface A palmtop PC interface provides the convenience of the familiar graphical windows operating system combined with a backlit color touchscreen for an unsurpassed user experiencein a thermal cycler The
3. 40 000 Supported consumables 384 well plates with an adhesive cover Dimensions H_220 mm 8 7 lid closed H_330 mm 13 0 lid open W 310 mm 12 2 D 295 mm 11 6 370 mm 14 6 including cables Weight 10 9 kg 24 lbs Electrical 100 120 VAC Amp 50 60 Hz 200 240 VAC 2 Amp 50 60 Hz Startup Accessories Blue Sillicon Pad Provided with 96 and 384 well configurations only Secure Key for Palm Device SD Memory Card 2 GB Power Cable User Manual Palmtop PC accessories manufacturer s standard set Software Supplied with unlimited user license Warranty 1 years on instrument manufacturers warranty on palmtop PC device Ordering Information cat Description Price G6000 000 CG Palm Cycler 60 well version supported consumables 0 5 ml tubes with attached caps CG9600 000 CG Palm Cycler 96 well version supported consumables 0 2 ml tubes or strip tubes with flat or domed cap 96 well high or low skirt plates with strip caps or an adhesive cover CG3840 000 384 well version supported consumables 384 well plates with an adhesive cover Thermal Cycler 14502 7000 Erai ino genetnbiotech com Webster www genetbiotech com z
4. ation reaction Itis most useful for targetslonger than about 1kb Oligo Calculator Palm Cycler software includes a full featured oligo calculator as an aid to oligonucleotide design In addition to a range of calculated values the software self aligns the oligo to show the occurrence of self complimentary bonds This can be used for example to identify palindromes and primer dimers Example oligo information is shown here A Scrolling the window down displays the self aligned bond information B Zero condensation Heated Gidig The Palm Cycler uses a heated lid to keep the void air volume in the tube hotter than the reaction see below This drives any evaporation and condensation back into the cooler reaction liquid Thus no oil or wax condensation barrierisneeded Heated Cover PCR Tube Reaction Mix Aluminum Block Peltiers Heated Lid Hot Bonnet Technology Thermal Calibration In some situations it may be desirable to have the Palm Cycler matched to an external thermal accuracy standard The software includes an auto calibration wizard in the Help menu to enable dynamic recalibration by the user The calibration process begins with measurement of Palm Cycler well temperatures using a thermocouple probe of the desired accuracy Measured temperatures are then used to overwrite existing Palm Cycler calibration settingsso that accuracy is matched to the probe Power Interruption and A
5. ng a run With the Palm Cycler a linear gradient of between 1 C and 24 C can be set across the block Unlike the Palm Cycler other thermal cyclers typically use fewer Peltier devices ad sensors A thermal gradient produced by these instruments can be very non linear having a fast thermal decay zone at one or more regions of the block An asymmetric gradient such as this makesit very difficult to predict actual sample temperature in each well and makes optimization experiments prone to error When enabled a programmable gradient from 1 C to 24 C can be setup from left to right on the Palm Cycler 8 Peltiers and 4 sensors enable a true linear gradient to be established Gradient technology allows the user to quickly identify the best possible set of annealing conditions in one run when optimizing cycling conditions Pener Pia um Sensors Shortly Palm Cyeler will be available with new Pharos Palm Top Support for all Reaction There are three models of Palm Cycler to suit different sample tube formats plates ow or high caps or adhesive Real time Graphs Palm Cycler software graphically displays the current measured temperature across the blockin real time as cycling progresses even for a thermal gradient A opposite Blue lines on the graph indicate target temperatures for each thermal sensor and the red lines report the measured temperature at each sens
6. or location Four sensors across the Palm Cycler black enable accurate interpolation of the temperature in each well Shown opposite B are the interpolated temperatures of all twelve columns across a 96 well block i e 12 columns x 8 rows as displayed in real time by the software Ramp Rate Control Thermal transition times or ramp rates can be precisely controlled by the Limit Rate function Limit Rate allows the user to specify the time taken to transition from one set temperature to the next Hold Lists The Hold function allows the creation of one or more pauses in the program With this feature active the unit will pause at the programmed point while emitting an alert beep The set temperature will be held indefinitely until a Continue button is pressed holds are useful if there is a need to remove tubes or add reagents at a particular point in a run skirt with strip film seals Programming Interface Cyding programs are created and edited in Program mode A program is graphically represented as shown opposite for a three step program A The touchscreen interface makes the Palm Cycler simple and intuitive to use displayed parameters such as temperature time step number or repeat number can be changed simply pressing on a displayed value to open an Edit dialog B opposite Miere zsaoc zre esze ssec esse Tearc sase ease iwase mase zare
7. power of a palmtop PC computer allows advanced software functions to be included and total flexibility in thermal cycle programming new software features are regularly incorporated True Linear Gradient Thermal gradient technology enables opposite sides of a thermal cycler block to be set to a different temperature The wells across the block then span the set temperature range creating a thermal gradient Agradient can be used for example to optimize the annealing temperature of an assay in a single experiment by simply determining the temperature of the wells that yielded the best result The Palm Cycler uses eight Peltier devices to actively heat and cool the block from 4 C up to 99 C Peltier devices are controlled by four separate thermal sensors arranged across the block The distribution of sensors enables a true linear gradient to be established via feedback loops from the sensors to the computer Furthermore Palm Thermal Gradient Cydlingi and available as free upgrades via web download An SD memory card is included for convenient backup and transfer of programs between instruments Each card can hold thousands of saved cycle programs In addition the calender software included with the palmtop PC computer makes a perfect booking system for laboratories where the instrumentisin high demand Cycler software can interpolate and display the temperature of each column of wells in real time duri
8. ul If for any reason the Palm Cycler is turned off the power fails or the palmtop PC is disconnected during a run the palmtop PC will sound a warning alarm for 30 seconds If reconnection occurs during that time the program will continue If not a Disconnected message appears onscreen indicating the name of the terminated program and the cycle step and repeat number at which interruption occurred Technical Specifical Thermal Cycling System Active heating and cooling using 8 Peltier elements 4 Platinum sensors Temperature Range a c 99 C Temperature Accuracy 0 25 C of set temperature 1 minute after clock start Temperature Uniformity 0 5 C 30 seconds after clock start Temperature Resolution 0 1 C increments Heating Cooling Rate 2 5 C sec maximum Condensation control Automatic using a heated lid design Special Functions Auto resume on power fail on line logging and graphing touchdown and long range programming linear thermal gradient programmable up to 24 O adjustable ramp rate oligonucleotide calculator program holds manual mode online help thermal calibration wizard Configurations 60 Well version CG 6000 000 Supported consumable 0 5 mL Tubes with attached caps 96 Well version CG 9600 000 Supported consumables 0 2 mL tubes or strip tubes with flat or domed caps 96 well high or low skirt plates with strip caps or an adhesive cover 384 Well version CG 38

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