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Installing KingFisher Duo Prime

1. KingFisher Duo Prime Technical Manual Rev 1 0 Cat no N16621 2015 Thermo Fisher Scientific Inc All rights reserved KingFisher Duo Prime magnetic particle processor and BindIt Software are registered trademarks of Thermo Fisher Scientific Inc Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history
2. folder Icon Description Viewing and defining date and time settings See Defining Date and Time Settings on page 49 Viewing the barcode reader settings Available when the S barcode has been connected to the device Last used protocol s are shown below the factory and user protocols folders The number of displayed protocols is limited by the available space The info text bar shows explanatory information on how to proceed and which keys to use Navigating This section describes navigation in the KingFisher Duo Prime user interface The main view changes according to the selections you make either with the Up or Down arrow keys or the OK button To move into the submenus use the OK button The available buttons and their function are shown on the info text bar 30 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific 4 Operation Control Panel The color of the items in the main view for example the icon and main view row change when they are selected active or inactive 09 04am V DNA PO RNA b Protein Other DNA RNA Protein Cell Other ESiKF_BloodDNA Duo Select Q Start El Details Figure 18 Active row To move from one menu to another make sure you are in the main view of one of the menus and use the Left and Right arrow keys To return from the submenus to the main views use the Left arrow key Thermo Fisher Scientific Thermo Scientific KingFisher
3. in a well ventilated room wearing disposable gloves protective glasses and clothing Perform decontamination in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed It is strongly recommended to perform the complete decontamination procedure before relocating the instrument from one laboratory to another or before sending it to service Example of decontaminants A GD Ethanol 70 Virkon solution 1 3 Glutaraldehyde solution 4 Chloramine T Microcide SQ 1 64 Decon 90 min 4 WARNING The decontamination procedure can only be performed by authorized trained personnel wearing disposable gloves protective glasses and clothing in a well ventilated room Prepare the decontaminant 200 ml 4 glutaraldehyde solution or another agent recommended by your safety officer Empty the turntable Switch OFF the power and disconnect the mains supply cable Disinfect the outside of the instrument using a cloth dampened with 70 ethanol 5 Place the instrument in a large plastic bag Ensure that the front lid is open Thermo Fisher Scientific Place a cloth soaked in the prepared solution into the bag Ensure that the cloth does not come into contact with the instrument Seal the bag and leave the instrument in the bag for at least 24 hours You can use the UV lamp to decontaminate the process chamber For more informatio
4. For more information see chapter Ordering Information on page 75 Tip Combs Specially designed tip combs that protect the magnets during the process are available for different plate types and applications To handle KingFisher Duo Prime 12 tip combs correctly 1 Take an individual KingFisher Duo Prime 12 tip comb from the package see the following figure Figure 37 KingFisher Duo Prime 12 tip comb 2 Unpack the plastic bag and place one of the tip combs onto the tip row B of the KingFisher 96 deep well plate see the following figure Avoid bending the tip combs to ensure proper instrument operation If the tip comb is bent during storage bend it slightly to straighten it 50 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific 4 Operation Handling Consumables Figure 38 Pacing the KingFisher Duo Prime 12 tip comb To handle KingFisher Duo Prime 6 tip combs correctly 1 Take a KingFisher Duo Prime 6 tip comb package where four unattached tip combs are packed onto a KingFisher Flex 24 deep well plate 2 Place one of the tip combs onto the tip row row B of an empty KingFisher Flex 24 deep well plate see the following figure Figure 39 KingFisher Duo Prime 6 tip comb inserted 3 Before you use the tip comb press the tip comb evenly by hand agains
5. Signature Name block capitals PHOTOCOPIABLE 80 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific Appendix B System Log Instrument name and number User Date Comments PHOTOCOPIABLE Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 81 10 Appendix B system Log 82 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific
6. 3 Export Device Report Date sone _ Export Run Log Time cm le Import Protocol pr js Export Protocol Date format dd mm yyyy Maintenance Protocols M 9 Time format 12h lt 2 Information Q Language Buzzer 2 Date and Time Barcode Reader Select Select 4 Back EM Date and Time EM Set date Figure 36 Setting date and time 3 Use the Up and Down arrow keys to select the date the time the date format or the time format and then press the OK button e To set the correct day month and year use the Up and Down arrow keys When you have the correct value for each press the OK button e To define the correct time use the Up and Down arrow keys When you have the correct value press the OK button e To select the desired date format use the Up and Down arrow keys The available date formats are dd mm yyyy ddlmmlyyyy dd mmyyyy mm dd yyyy and yyyy mm dd The default is dd mm yyyy When you have the correct value press OK e To select the wanted Time format 12h or 24h use the Up and Down arrow keys When you have the correct value press the OK button 4 To cancel or to return to the Maintenance menu press the Left arrow key Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 49 4 Operation Handling Consumables Handling Consumables This chapter advises on how to handle the KingFisher Duo Prime consumables such as tip combs plates and elution strip
7. 98060196 KingFisher Pure RNA Plant Kit 96 98060496 KingFisher Pure RNA Plant Kit 384 98070196 KingFisher Pure Viral NA Kit 96 98070496 KingFisher Pure Viral NA Kit 384 98080196 KingFisher Pure Plasmid Kit 96 98080496 KingFisher Pure Plasmid Kit 384 Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 77 8 Ordering Information KingFisher Pure Kits 78 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific Appendix A Certificate of Decontamination Name Address Tel Fax Instrument Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological Radioactive Special information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place l Please include decontamination solution used Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 79 9 Appendix A Certificate of Decontamination
8. Duo Prime Technical Manual 31 4 Operation Control Panel The main views of each menu tab are shown in the following figure J gt K 09 04am Ej vr 09 09am EP K 09 09am Y NY DNA Run Log Q UV Lamp RO RNA ESKF BloodDNA Duo 3 Export Device Report ait Protein 44 19 01 2015 08 28 am _y Export Run Log Other gt he Import Protocol 5 M DNA RNA Export Protocol y Protein e Maintenance Protocols W Cell 4 14 Information Other Q Language Ly amp KF BloodDNA Duo Buzzer Y Date and Time Barcode Reader y Select Start Select l Details y Select Em r i Mins Figure 19 Main views Using KingFisher Duo Prime PC Software You can also control the operation of the KingFisher Duo Prime magnetic particle processor by an external computer and run on the BindIt Software In addition to the KingFisher Duo Prime internal software features you can download protocols to the instrument or back up protocols from one instrument and transfer them to another For more information see the BindIt Software User Manual Cat no N07974 Using Internal Software This section describes procedures concerning the KingFisher Duo Prime internal software Factory or User Protocols This section describes the factory or user set protocols found in the Protocols menu Factory protocols are protocols which are created by Thermo Fisher Scientific and preloaded into the instrument internal memory in
9. Duo Prime Technical Manual Thermo Fisher Scientific 5 Maintenance Packing Instrument for Transportation or Service WARNING Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The procedures vary by ES country Pollution degree 2 for more information see Safety specifications in Chapter Technical Specifications Method of disposal Electronic waste Contaminated waste Infectious waste Regarding the original packaging and packing materials use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 65 5 Maintenance Packing Instrument for Transportation or Service 66 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific Technical Specifications General Specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program The general specifications are presented in the following table For information on accessories see Accessories and Consumables on page 76 Table 6 General specifications Item Overall dimensions e instrument transport package Weight e instrument e instrument with transportation package Operating conditions indoor u
10. Duo Prime folder which is automatically created on the USB memory device If you transfer protocols from the USB memory device to the instrument they must already be in the correct folder named KingFisher Duo Prime When the USB memory device is plugged in a small memory stick icon appears at the bottom right corner of the window You can also transfer protocols using the BindIt Software For more information see the BindIt Software User Manual Cat no N07974 Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 39 4 Operation Viewing and Defining Instrument Options and Settings Switching UV Lamp On You can use the UV lamp to decontaminate the process chamber If you open the front lid the UV lamp shuts down UV radiation is useful in decontamination because of its ability to inactivate biological molecules KingFisher Duo Prime is equipped with a UV lamp that can be used to treat the interior of the instrument with UV light to help with decontamination after cleaning Note that UV treatment is not a substitute for cleaning Using the UV lamp treatment alone may not provide sufficient decontamination For cleaning instructions see Decontamination Procedure on page 61 To switch on the UV lamp 1 Go to the Maintenance menu 2 Select the UV Lamp row and press the OK button 4 Q UV Lamp Set time 00 01 Total hours 0 O Start lt Back E Set time Figure 27 Switchin
11. II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 69 6 Technical Specifications safety Specifications Note 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation 70 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific Troubleshooting Note Do not use the instrument if it appears not to be functioning properly When an error is detected terminate the current operation After an error occurred abort the current run and restart from the beginning when the problem is fixed The following table provides a troubleshooting guide for the KingFisher Duo Prime instrument Table 9 Troubleshooting guide Code s 2 3 260 Description The command was not recognized as a valid command The tip comb holder lifting mechanism is out of position The track mechanism is out of position The turntable rotating mechanism is out of position The magnet head holder
12. Maintenance Packing Instrument for Transportation or Service Figure 48 Placing internal cushions Service Contracts It is recommended to maintain and service the instrument regularly every 12 months on a contract basis by the manufacturers trained service engineers This ensures that the product is properly maintained and gives trouble free service For more information contact the Thermo Fisher Scientific technical service department Maintaining System Log To properly maintain the system the system log is useful It contains a short summary of the use maintenance procedures error messages and other information on the use of the system For more information see Appendix B on page 81 Copy the table as many times as necessary but leave the blank original inside the user manual Disposal of Instrument If the KingFisher Duo Prime has to be disposed of follow the guidelines given in this section WARNING Decontaminate the instrument before disposal For more information see Decontamination Procedure on page 61 Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal local regulations The Li battery has to be changed by an authorized service technician only X WARNING The used lithium Li battery is regulated waste and must be disposed of according to Es Instructions for changing the Li battery are described in the service manual 64 Thermo Scientific KingFisher
13. User Manual e KingFisher User Manuals CD e KingFisher Duo Prime Technical Manual this manual e Thermo Scientific KingFisher Duo Prime Brief User Manual and translations into French German Italian Spanish Portuguese Russian Japanese and Chinese e Thermo Scientific User Awareness of Symbols e Declaration of Conformity document Note Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so might cause misalignment and will void the instrument warranty Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 15 3 Installation Removing Internal Cushions CAUTION The KingFisher Duo Prime weighs approximately 17 kg 37 5 lbs without the transport package and must be lifted with care It is recommended that two persons lift the instrument together taking the proper precautions to avoid injury To lift the instrument place your fingers under the device on both sides and lift it with your back straight Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use Removing Internal Cushions When you have opened the package open the front lid of the instrument and remove the two interna
14. contact service Try another USB memory device If the error still appears contact service Possible NTFS formatted USB memory Change the memory or reformat to FAT Contact authorized technical service Contact authorized technical service Contact authorized technical service Contact authorized technical service Contact authorized technical service Switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service Close the front cover Thermo Fisher Scientific Table 9 Troubleshooting guide Code s 321 322 323 Thermo Fisher Scientific Description Protocol aborted by user Clock battery is empty Aborted because there was an error in another protocol Troubleshooting Action No action needed For information only Contact authorized technical service No action needed For information only Thermo Scientific KingFisher Duo Prime Technical Manual 73 1 Troubleshooting 74 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific E Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Ordering information codes are presented in the following tables KingFisher Duo Prime The following configurations of the KingFisher Duo prime system are available Table 10 Codes for products Code Instrument System 5400110 KingFisher Duo Prim
15. or changing the magnet heads on page 22 Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 11 2 Functional Description Heating Blocks Heating Blocks KingFisher Duo Prime is equipped with two different kinds of interchangeable KingFisher Duo Prime heating blocks with two pieces each a 12 well heating block for Microtiter DW 96 plates and a 6 well block for the KingFisher Flex 24 DW plates see Table 2 You can add a heating step of 10 C to 75 C to a protocol Row A of the above mentioned 24 and 96 DW plates can be heated You can also heat the elution strip position Table 2 Plates and heating adapter s Plate Description KingFisher Flex 24 deep well DW plate 200 5 000 pl Microtiter deep well 96 plate 50 1 000 ul KingFisher Duo Prime elution strip 30 130 pl Heating adapter s Description KingFisher Duo Prime heating block for 24 DW plate KingFisher Duo Prime heating block for 96 DW plate KingFisher Duo Prime heating block for elution strip fixed Heating cooling temperature range 10 to 75 C 10 to 75 C 4 to 75 C Note If the filling volume is not followed it is below or above the given value adequate performance is not guaranteed 12 Thermo Scientific KingFisher Duo Prime Technical Manual WARNING Risk of burns The heating block and or the elution block surface can be hot The warning LEDs have a red light when t
16. particle size Magnet rods Plates per deck Plate types disposable Recommended filling volume Elution strip Tip combs polypropylene disposable Automatic tip load Heating cooling temperature plate row block elution strip block Heating block accuracy Keypad Display Thermo Scientific KingFisher Duo Prime Technical Manual Value 96 DW plate 12 pin magnet head 50 1 000 pl Elution strip 12 pin magnet head 30 130 pl 24 DW plate 6 pin magnet head 200 5 000 ul 200 5 000 ul performance may vary depending on the mixing speed and liquid 12 pin magnet head up to 12 6 pin magnet head up to 6 24 uninterrupted if two runs are ongoing at the same time with a 12 pin magnet head gt 95 Microtiter deep well 96 plate neutral wash buffer containing detergent 2 8 um particles 3 collections RT ca gt lum 12 or 6 interchangeable KingFisher Duo heads 2 Microtiter deep well 96 plate 50 1 000 ul KingFisher Flex 24 deep well plate 200 5 000 pl Special design 1 x 12 format 12 6 in one frame for Microtiter deep well 96 plate or KingFisher Flex 24 deep well plate Yes e From 10 C to 75 C instrument in RT e From 4 C to 75 C instrument in RT 1 C up to 75 C instrument in RT START PAUSE STOP OK ROTATE four arrow keys amp BACK Left arrow key LCD 5 480 x 800 pixel color display Thermo Fisher Scientific 6 Technical Specifications safety Spec
17. 41 4 Operation Viewing and Defining Instrument Options and Settings o 4 02 00pm b gs 02 02pm Q9 UV Lamp Q9 UV Lamp Export Device Report Export Device Report 4 Export Run Log a Export Run Log Ls Import Protocol L Export Protocol he Import Protocol L Export Protocol Export OK Device report exported to 1 d cmon no the USB memory Attach USB memory or Press M 4 press 4 to cancel lt Barcode Reader TUTUUC Ac Select KA Export Device Report Select EA Export Device Report Figure 29 Exporting the instrument report Exporting Run Log The serial number of KingFisher Duo Prime is in the header of the run log The report includes the protocol name the time when the protocol was started and stopped and the plates and strips used of the all the user and factory protocol runs as well as those of all the UV protocol and maintenance protocol runs You can export the run log To do this a USB memory device has to be plugged into the instrument Run reports are exported in CSV format which can be opened in excel All runs from the export time are included as well as all fields from each run 4 To export the run log 1 Go to the Maintenance menu 2 Select the Export Run Log row and press the OK button The following figure illustrates the E messages shown when the export is successful or unsuccessful 42 Thermo Scientific KingFisher Duo Prime Technical Manual T
18. Elution 4 Wash1_1 5 Wash1_2 Stop Q Stop Dispense Figure 23 Viewing the progress of the protocol Q To terminate the run press the Stop button You are asked to confirm the termination or to continue Note that when you pause the operation by pressing the Pause button during a run the Pause O icon flashes on the menu bar after the Pause message appears on the display ii e In addition the run can include pauses created by the protocol itself such as a pause to change plates load a plate unload a plate and dispense Press the OK button to continue 36 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific 4 Operation Control Panel KF_BloodDNA_Duo KF_BloodDNA_Duo Add 25 ul Beads and 400 yl Binding Buffer to row A E Press EM to continue zu 00 30 E 00 30 2 Dispense 1 Lysis 3 Bind 2 Dispense f 4 2 Wash1_1 3 Bind Wash1_2 4 Wash1 1 6 75 Wash Ethanol 5 Wash1_2 O Stop Q Stop Dispense Dispense Figure 24 Viewing protocol status In the previous figure the following items are shown Item Description 1 Time left before the next pause 2 Remaining run time shown on the time bar 3 Steps bar During the run the remaining run time is shown in smaller numericals on the time bar The time shown in bold and larger numericals in the middle of the window indicates the time remaining until the following pause During the pause
19. Figure 4 on page 5 A USB cable is supplied with the instrument USB Ports for Memory Devices and Barcode Reader There are two parallel USB ports one on the back panel and one on the front panel see Figure 3 on page 4 The USB ports are intended to be used with a memory stick for transferring import and export protocols and for exporting run log files for traceability PC connection is not necessary You can use either one of the ports to attach a barcode reader to the equipment but for better usability the port on the back panel is preferred if the connection in the front is used for the memory stick It is recommended to use FAT16 or FAT32 formatted USB memory devices It is not recommended to use multiple drive USB memory devices that contain virtual CD drives Consumables For more details and ordering information on plastic consumables such as plates tip combs and elution strips used with the KingFisher Duo Prime instrument see Accessories and Consumables on page 76 Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 13 2 Functional Description Reagents Reagents There is a wide selection of optimized Thermo Scientific KingFisher Pure Kits for low or medium throughput available for purification of DNA or RNA The versatile kit selection is intended for a large variety of starting materials such as blood cells tissues or cell free body fluids The DNA or RNA purified with
20. Fisher Duo Prime can be used with one processing head at a time The instrument comes equipped with two different kinds of interchangeable KingFisher Duo Prime magnet heads a 12 pin magnet head for Microtiter deep well DW 96 plates and a 6 pin magnet head for KingFisher Flex 24 DW plates The magnet head options have been designed for different volume needs Both KingFisher Duo Prime heads have corresponding disposable plastic tip combs see Table 1 on page 10 The 12 pin magnet head is used with a corresponding plastic 12 tip comb and a Thermo Scientific Microtiter DW 96 plate and an optional 12 well Thermo Scientific KingFisher Duo Prime elution strip e 12 pin magnet head e Oneto 12 samples per run e Eight processing positions including the elution strip e Upto 1 ml processing volume Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 9 2 Functional Description Magnet Heads The 6 pin magnet head is used with a corresponding plastic 6 tip comb and a Thermo Scientific KingFisher Flex 24 DW plate e 6 pin magnet head One to six samples per run e Three processing positions optionally with two plates up to seven positions e Upto 5 ml processing volume Note The tip comb uses one processing position from the plate in both cases 12 pin and 6 pin CAUTION Do not place the KingFisher Duo Prime heads on top of the instrument or any metal surfaces Figure 8 Keep the Kin
21. March 2015 First release for KingFisher Duo Prime For Research Use Only Not for use in diagnostic procedures WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equip ment WEEE Directive 2002 96 EC It is marked with the following symbol T in Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product Contact Thermo for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country Preface About This User Manual This user manual is written for the actual end user for example research scientist or laboratory technician This manual provides information on the Thermo Scientific KingFisher Duo Prime magnetic particle processor including the installation and operating instructions This document aims to give you the information you need for Reviewing safety precautions Installing the KingFisher Duo Prime Using the KingFisher Duo Prime in routine jobs the processing step Performing basic cleaning and maintenance procedures e Troubleshooting the instrument performance This user manual also describes the features and specifications of the KingFisher Duo Prime instrument as well as ordering information Note that simulated data appears in the scre
22. ake a task easier iv Thermo Scientific KingFisher Duo PrimeTechnical Manual Thermo Fisher Scientific Preface safety and Special Notices Safety Symbols and Markings on KingFisher Duo Prime The following symbols and markings appear on the type label and the instrument itself WARNING UV light WARNING This product contains very strong permanent magnets WARNING Risk of burns The heating block and or the elution block surface can be hot The warning lights are lit if the temperature rises too high WARNING Risk of injury because of moving parts Instrument Safety and Guidelines for Use Take into account the following instrument safety considerations and guidelines for correct use Before operating the instrument read this user manual in its entirety Failure to read understand and follow the instructions in the manual may result in damage to the instrument injury to laboratory and operating personnel or poor instrument performance e Always follow basic safety precautions and local safety guidelines when using the KingFisher Duo Prime to reduce the risk of injury biohazardous contamination fire or electric shock Before operating the instrument read this user manual in its entirety Failure to read understand and follow the instructions in the manual may result in damage to the instrument injury to laboratory and operating personnel or poor instrument performance e Observe all Warning Caution
23. and Note statements as well as safety symbols and markings on the instrument and in the documentation The device is allowed to be operated only with software specifically designed for the device Thermo Fisher Scientific Thermo Scientific KingFisher Duo PrimeTechnical Manual v Preface Contacting Us e Never open any other covers of the KingFisher Duo Prime than the front lid or the maintenance door see Figure 2 on page 3 while the instrument is plugged into a power source e Never force a microplate onto the instrument e The KingFisher Duo Prime is intended for laboratory research use only Observe proper laboratory safety precautions such as wearing protective clothing and following approved laboratory safety procedures e Preventative maintenance instructions should be followed closely to keep the instrument in the best condition for maximum reliability A poorly maintained instrument does not give the best results Contacting Us For the latest information on products and services visit our website at www thermoscientific com kingfisher vi Thermo Scientific KingFisher Duo PrimeTechnical Manual Thermo Fisher Scientific Contents Chapter 1 Chapter 2 Chapter 3 Thermo Fisher Scientific PIOlACO iia ELTE UIT NT ii About This User Manual o RR eee ee iii Related Documentation 0 0 00 cece ce eee ooo o oo iii Safety and Special Notices eeu ope nt ropas popa bs iv Safety Symbols
24. and Markings on KingFisher Duo Prime M Instrument Safety and Guidelines for Use llle V Sora i Hr vi Introduction to KingFisher Duo Prime s elles 1 Intended Usa sx 2d dudas ae hue yd edd det bse 8 deeds ee dT l rcu Music EN E E ET TEET EEEE 1 Functional Description 0 ccc cece reece eee eee eens 3 Iistru ment b uuo PP pra hase eee nee eo 3 FROME VIEW gions ean rior algae ee oe Sade ee eed ase On cease es 3 Back and Internal View vada varados patata peewee tend eaeeeseees 5 King Fisher Duo Prime Magnetic Particle Processor o oooooooooo 6 Principle of Magnetic Particle Processing oo ooooooooomommomoo 7 Working with Magnetic Rod 1 eee ees 8 Collecting Magnetic Particles can 5Gbecsetenedicedbisigehaeneeer ds 8 Releasing Magnetic Particles 222 ee EE Rr rk E po aden 8 Washing Magnetic Particles and Incubation 0005 8 Changing Volume During Magnetic Particle Processing 9 Magnet Meigs reseta t ces ce ede eee PEDE e pda seen ratas ee 9 Heatime Blocks o cag eseeae te sd ope E EEEE EEEE E 12 Plinio Blocs ee cosranci n bobas eds PEE EE 13 SMC le acne RECETTE ETT ET TR TET eel Ba Rees pas 13 USB Pomor PC iocus cosita ros orar rana ta 13 USB Ports for Memory Devices and Barcode Reader o o o oomoooo 13 Sri OSs sonum hentir RE pee 6 Ge eee ee eee es nel gre NI a a EE 13 gun AA 14 IGI Sars cerros depri
25. ates elution strips and caps for elution strips for 96 samples Microtiter deep well 96 plate 50 Microtiter deep well 96 plate sterile 50 KingFisher Flex 24 deep well plate 50 KingFisher Flex 24 deep well plate sterile 50 76 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific 8 Ordering Information spare Parts Spare Parts The following spare parts are to be used with the KingFisher Duo prime instrument Table 12 Codes for spare parts Code Item Quantity pieces N12459 KingFisher Duo head for 96 DW 1 N12460 KingFisher Duo head for 24 DW 1 N12461 KingFisher Duo heating block for 96 DW plate 1 N12462 KingFisher Duo heating block for 24 DW plate 1 N16539 Lamp UV C Germicidal T5 16x288 mm 8W 1 2305290 Serial cable F9 F25 for RS 232C port 1 N04001 USB A B device cable 1 8 m 1 Longer USB cables available from PC stores KingFisher Pure Kits The following optimized DNA RNA purification kits for the KingFisher Duo Prime are available Table 13 Codes for purification kits Code Item Quantity pieces 98010196 KingFisher Pure DNA Blood Kit 96 98010496 KingFisher Pure DNA Blood Kit 384 98020196 KingFisher Pure RNA Blood Kit 96 97030196 KingFisher Cell and Tissue DNA Kit 96 97032496 KingFisher Cell and Tissue DNA Kit 24 x 96 98040196 KingFisher Pure RNA Tissue Kit 96 98040496 KingFisher Pure RNA Tissue Kit 384 98050196 KingFisher Pure DNA Plant Kit 96 98050496 KingFisher Pure DNA Plant Kit 384
26. b and plates The instrument functions without any dispensing or aspiration parts or devices Samples and reagents including magnetic particles are dispensed into the plates according to the corresponding instructions The protocol that you have selected with the keypad and display has already been transferred onto the onboard software You can use the Thermo Scientific BindIt Software to create and run protocols Thermo Fisher Scientific Thermo Scientific KingFisher Duo PrimeTechnical Manual 1 1 Introduction to KingFisher Duo Prime Figure 1 KingFisher Duo Prime magnetic particle processor To keep the instrument in the best condition for maximum reliability follow the preventative maintenance instructions A poorly maintained instrument does not give the best results For more information on maintenance see chapter 2 Thermo Scientific KingFisher Duo PrimeTechnical Manual Thermo Fisher Scientific 2s Functional Description Instrument Layout This section shows the front back and internal views of the KingFisher Duo Prime instrument Front View The following figure illustrates the KingFisher Duo Prime instrument and its parts listed in the table that follows Figure 2 KingFisher Duo Prime front view with front lid open Item number Description Item number Description 1 Front lid open 4 Elution block fixed 2 Maintenance door 5 Interchangeable heating block 3 Interchangeable KingFisher 6 Turnta
27. ble Duo Prime head Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 3 2 Functional Description Instrument Layout The following figure shows the KingFisher Duo front view with plates and front lid and the maintenance door closed o WOW Figure 3 KingFisher Duo Prime front view with plates and front lid closed Item nnper Description 1 Maintenance door 2 Shield plate Cooling air outlet Display 5 Keypad 4 Thermo Scientific KingFisher Duo Prime Technical Manual Item number 6 7 10 Description Front lid closed USB port for memory stick or barcode reader USB port also at the back of the device ON OFF switch UV lamp USB port and barcode reader connection preferred location Thermo Fisher Scientific 2 Functional Description Instrument Layout Back and Internal View The back view of the KingFisher Duo Prime instrument is shown in Figure 4 and the internal view in Figure 5 Figure 4 KingFisher Duo Prime back view Item number Description Cooling air inlet 2 USB port for PC 3 USB port for memory stick or barcode reader 4 Mains power supply connector 5 Transport lock 6 Type label Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 5 2 Functional Description King Fisher Duo Prime Magnetic Particle Processor The following figure shows the KingFisher Duo Prime internal view from the front processing posit
28. col and place the plate onto the turntable The processing of the second plate starts automatically when the first one is completed 24 samples at most can be purified continuously without an interruption by the user and in this case the samples are processed in 96 deep well DW plates When using large volume configurations on 24 well plates usually two plates are used in a run Before starting the magnetic particle processing with the keypad and display the samples and reagents are dispensed into the plate s strip s and the tip comb is placed onto the preset row of the sample plate default row B from which it is automatically loaded The plate s are placed onto the turntable into the corresponding plate position according to the protocol instructions During the operation the front lid can be closed or open see Figure 2 on page 3 and Figure 3 on page 4 The closed door protects the processing against environmental contamination Principle of Magnetic Particle Processing The operating principle employed is inverse magnetic particle processing MPP technology see Figure 6 Rather than moving the liquids the magnetic particles are moved row wise on the plate s or strip s containing specific reagents in contrast to the external magnet method Magnetic particles are transferred using magnetic rods covered with a disposable specially designed plastic tip comb Fast up and Magnet down releasing Magnet movement Slow up and
29. d until all the particles have been mixed with the substance in the next reaction Washing Magnetic Particles and Incubation Washing the magnetic particles is a frequent and an important processing phase Washing is a combination of the release and collection processes in wells filled with washing solution To maximize washing efficiency the magnetic rods together with the tip comb are designed to have minimized liquid carrying properties To keep the magnetic particle suspension evenly mixed in incubating long running reactions the tip comb can be moved up and down in the solution 8 Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific 2 Functional Description Magnet Heads Changing Volume During Magnetic Particle Processing The volume of the first row of the plate can be larger than the volume of the next row and this can be used for example to eluate the sample in a smaller volume to get a more concentrated end product Fast up and down releasing Magnet movement see the following figure Magnet Slow up and down collecting movement is Tip a gt Reagent 1 amp Reagent 2 amp magnetic particles magnetic particles I Well 1 3 Well 2 Figure 7 A concentration step during magnetic particle processing Magnet Heads The King
30. dada oe hes 48 Defining Date and Time Stings odes donee pet ewe REA URS 49 Handling Consumables soser v id wee tee ieee Ea Rt ase deed ut 50 TOSCO ETC IP 50 IE EIS awit poets hh lie Batis SES TIU EE E EET 52 Poton SUL tient Game t oboe Ra uerne ead oe ete a 53 Starting KingFisher Duo Prime sia 32 e tide e 60 dee eee see eae te nde 54 SLOP PINS suoi aE d A Acme cedi E A eR ied issus DA ETHeIe eucy UIO OS uated ob iuo Ad Ux d axe Med 2 Chapter5 Maintenance occccccccc III 59 Regular and Preventive Maintenances s es i Ped db en dob ob ob e edd 59 Cleanins Turntable uses statu etate Pei a oe ee dh RE 60 Cleaning Magnetic ROdSss a4 obe e Ev es lios 60 Cleanine Shield plate aa tus a duas EE aA SHE ge 60 I9isposaloE Materials on P theese whe eode aem Pd Sup ui cear ted et 60 Decontamination Procedure cee cee cee eee hen 61 Packing Instrument for Transportation or Service 2 2 0 0 eee eee ee 62 Retittno Transport hock i525 emai caw da tee cet bg nid 62 Placing Internal Cushions ia ietrb e e ini oa Mice EARN a eee Be eas 63 Serios Contracts aros arto estara acia orina dus sio oe eed 64 Maintaining System Lop s Seria ee ee il rep iti she Ier EA 64 Diposaken nE sco 222 456 ira 64 Chapter6 Technical Specifications 0 cece cece eee ees 67 General Specii adon dai n ute M addu eee ys cat UR oe 67 Performance Specifications O A a 68 viii Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fi
31. down collecting movement Tip Collection of magnetic particles Release of magnetic particles into a from a large volume well low volume well He 1 y E Well 2 3 Figure 6 Inverse magnetic particle processing Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 7 2 Functional Description Working with Magnetic Rod Working with Magnetic Rod Working with magnetic particles can be divided into five separate processes 1 Collecting magnetic particles 2 Releasing magnetic particles 3 Washing magnetic particles 4 Incubation 5 Concentration Collecting Magnetic Particles During the collection of the magnetic particles the magnetic rod is fully inside the tip The magnetic rods together with the tip comb move slowly up and down in the plate and the magnetic particles are collected onto the edge of the tips Having collected the magnetic parti