NICHIRYO Le 本文（J/E) - Fisher UK Extranet
Contents
1. een dec ame Te MoNooOoNOIN perator s sampling skill Solution Clean or replace the nozzle cylinder part The nozzle cylinder is loose Retighten the bottom nut The O ring is worn Replace the O ring Suctioned liquid leaks from the tip The O ring is worn Replace the O ring Airtight section lubricant grease has been used up Grease the airtight section Nozzle cylinder point is worn Visual inspection shows level difference Replace the nozzle cylinder Plunger surface is scratched or amaged Replace the plunger he tip being used does not fit the Change the tip used Push button operation is poor unger O ring lubricant grease has d T nozzle P been used up Grease the plunger and O ring iquid has been drawn into the unit inside of nozzle cylinder Disassemble the lower part and clean Plunger surface is dirty or foreign matter has adhered to it Disassemble the lower part and clean or replace the plunger f you experience any problems other than those noted above or if there is still a problem even after carrying out the recommended solutions please stop using the instrument immediately and request service At that time please be sure to make sure that the instrument is not contaminated with any substances such as microbes or chemicals that are harmful to the body mReplacement parts list Service parts list2. pipetting 4 Operate the push button gently If quickly released sample liquid will be sucked into the main body which may result in not only inaccurate pipetting but also deterioration of the instrument 5 Do not hold the instrument horizontally or upside down when there is liquid inside the tip otherwise the liquid will get inside the main body which may cause deterioration of the instrument 6 If liquid is sucked inside the main body inside the nozzle clean as quickly as possible Liquid inside the nozzle is a main cause of precision reproducibility loss and may lead to breakdown of the instrument 7 When turning the push button do not exceed the specified sample volume limit as this may damage the instrument or cause deterioration 8 Donot perform pipetting with less liquid than the set volume If the quantity is less than the set volume this may cause the liquid to scatter into the main body which may result in deterioration of the instrument V Operation 1 Volume setting For each volume the graduation consists of three numerical values that are read from top to bottom Turn the push button to the left or right aligning it with the numerical value of the desired volume k Graduation units of each model are as shown in the chart below Volume graduation unit chart NLE 10 aa 20 u 100 s 200 NLE a LEN EM LNI mm L 1 mm L I 9 5u 16 5u 95 u 165u T Fig A 0 95m Observe the foll
3. France Belgique Belgi Fisher t l 0388671414 tel 056260260 Bioblock Scientific 0388671168 fex 056260270 email infos bioblock fr email belgium bioblock com Parc d innovation BP 50111 F67403 illkirch cedex www bioblock com www bioblock be Mode d emploi Espa a tfno 915159234 fax 91 515 92 35 email ventas bioblock com www es fishersci com Light amp Ergonomic Pipette NICHIRYO L Digital micro pipette for liquid handling User s Manual CERTIFIED 1509001 Always Pursuing Originality Since 1944 NICHIRYO Light amp Ergonomic Pipette NICHIRYO LE Thank you for purchasing the NICHIRYO Le Before proceeding to work with the NICHIRYO Le please carefully read this manual for proper use of it I Introduction This operator s manual explains the operation of the Nichiryo Digital Micro Pipette NICHIRYO Le Please read carefully to ensure safe and correct operation of the instrument Please pay special attention to passages marked in bold lettering Be sure to read cautions that need strict observance and underlined sections before using the instrument ll Features As volume amount setting is done digitally easy volume setting is possible with a simple press of the push button Through adoption of an easy to grasp design and a lightweight construction in comparison with our Nichipet EX model we have realized a product design that allows long hours of use without operator fatigue The s
4. Part No Part name Nozzle cylinder P lease state volume size Bottom nut Same for all volume sizes Ejector pipe ease state volume size Plunger ease state volume size First spring lease state volume size First spring holder lease state volume size Stroke ring lease state volume size Second spring lease state volume size O ring retainer P P P P P P P ease state volume size O ring P ease state volume size As the above replacement parts are specifically designed for each volume size please indicate the volume size of your instrument when placing your order sNichiryo Genuine Tips e Tips Autoclavable Volume range Color ul 0 1 10 Applicable model NLE 10 Tiplength Number of approx size tips 1000 tips 2 200 NLE 20 100 200 1000 tips 100 1000 NLE e Racked tips Autoclavable Volume range ul BMT SSR 0 1 10 1000 Applicable model NLE 10 1000 tips Number of tips 960 tips 96 tips x 10 cases BMT SGR 2 200 NLE 20 100 200 960 tips 96 tips x 10 cases BMT LR 100 1000 NLE 1000 1000 tips 100 tips x 10 cases For repair service or information please contact your local distributor MANUFACTURER NICHIRYO 4 10 Iwamoto cho 2 chome Chiyoda ku Tokyo 101 0032 Japan
5. TEL 81 3 5820 2664 FAX 81 3 5280 2410 Homepage URL http www nichiryo co jp E mail info nichiryo co jp 90 2004 7 3000
6. der First spring plunger first spring holder second spring and O ring The plunger and first spring holder are a complete set on the 1000ul model Once removed carry out part replacement inspection and cleaning of the airtight section CAUTION During disassembling take care not to lose any of the parts 2 Assembly k Basically assembly is done in the reverse order of disassembly Refer to Fig E 1 Set the O ring on the O ring holder 2 With the plunger tip facing upward attach each part in the order of first spring gt first spring holder gt stroke pipe gt second spring gt O ring retainer gt O ring Next insert in the nozzle cylinder 3 Install the ejector pipe Install in the reverse order of disassembly A CAUTION After assembly before using the unit test operate it a few times to confirm that there are no problems VII Specifications Volume range ul Code Measured volume ul Reproducibility NLE 10 1 10 5 1 10 20 c3 NLE 20 2 20 10 2 20 SE o NLE 100 10 100 10 50 100 3 NLE 200 20 200 20 100 200 NLE 1000 Accuracy and reproducibility of 1u will be greatly influenced by the o 100 1000 100 500 1000 VIII Trouble Shooting Problem Liquid is not suctioned Probable cause Inside of nozzle cylinder point is clogged foreign matter buildup HH HH HT HT HT A
7. e nozzle If not attached securely it may fall off causing the sample liquid to scatter When handling liquid harmful to the human body be careful not to directly touch the tip during or after use If the instrument is soiled with liquid harmful to the human body immediately take proper measures to clean it safely before proceeding with work Do not use this instrument for stirring liquid or for any other such action Additionally do not use the instrument in any manner that will cause the tip to loosen fall off or the instrument itself to be soiled with scattered liquid Do not use this instrument for any purpose except pipetting liquid Q Matters that require strict observance Users are required to strictly observe the following points in order for the instrument to retain its excellent precision reproducibility and original performance for a long time 1 Do not expose this instrument directly to the sun when working with it or for 2 hours before starting work otherwise the instrument may fail in precise pipetting Avoid working with this instrument in a humid and hot place 2 Just before starting work with this instrument avoid touching the tip and nozzle cylinder as far as circumstances permit If the nozzle cylinder is warmed by your hand it is hard to maintain accuracy 3 For pipetting follow the forward method the way explained in this manual If it is performed in a different way it may result in inaccurate
8. ith paper or something similar being careful not to touch the liquid with your hand Caution Do not suction liquid from the C position in Fig B Operate the push button slowly and smoothly If released quickly the liquid may be sucked into the unit resulting in an inaccurate liquid amount Never suction liquid directly from the nozzle point without the tip attached Fig B 3 Discharge 1 Place the tip point against the inside inner wall of the receiving vessel Fig C 2 Slowly depress the push button to the first stop position a gt b position in Fig B Wait about 1 second and then depress it further to the second stop position c position in Fig B Fig C 3 With the push button still depressed at the C position of Fig B remove the tip point from the receiving vessel while sliding it upward along the inner wall of the vessel When removing the tip point from the discharged sample be careful not to release the push button as liquid will be draw inside the tip again 4 Release the push button 5 Press the ejector button to remove the used tip and dispose of it CAUTION When handling liquid harmful to the human body be careful not to directly touch the tip during or after use Reference 1 Pre rinsing When attaching a new tip before sampling repeat suction and discharge of liquid to the first stop position 2 to 3 ti
9. mes Pre rinsing will result in a high degree of accuracy In addition especially when strict reproducibility is sought we recommend this method when sampling various types of liquid 2 Sampling high density liquids and viscous solutions When suctioning once the liquid has entered the tip wait 2 to 3 seconds before removing the tip from the liquid When discharging wait 2 to 3 seconds at the first stop position before pushing to the second stop position Fig D 4 Plunger 5 First spring 6 First spring holder 7 Stroke ring 3 Ejector pipe P 8 Second spring 9 O ring retainer 10 O ring VI Disassembly and assembly of airtight section For airtight section maintenance or when a problem such as that clearly stated in the Trouble Shooting section of this manual occurs carry out disassembly inspection and cleaning following the instructions below 1 Disassembly 1 Remove the ejector pipe With the ejector button depressed slide the ejector pipe to the right clockwise direction and remove from the ejector shaft Next pull out the ejector pipe Refer to Fig D 2 Remove the bottom nut While holding down the nozzle cylinder turn the bottom nut counter clockwise and remove from the body At this time the spring inside the nozzle cylinder may cause the internal parts to pop out Please use care and remove slowly 3 Remove each part Remove the parts in the nozzle cylin
10. owing cautions when setting volume D When setting to an increased volume gt First exceed the desired setting graduation by about 1 3 revolution then adjust the indication to the desired graduation 2 When setting to a decreased volume gt Slowly turn to the precise point being careful not to go past the desired graduation setting A Caution Do not exceed the stipulated volume range or change the volume capacity This could damage the instrument or impair quality Never turn the push button more than 1 3 revolution past the maximum stipulated volume Suction Forward method Attach the disposable tip to the nozzle end Make sure that it is attached securely we recommend that you attach the tip from the rack Press the push button down to the first stop position ab position in Fig B With the push button depressed insert the tip vertically into the liquid to be suctioned Insert to a suitable depth about 3mm Fig C Slowly release the push button to its original position Fig B position a drawing the liquid inside At this time after the push button is returned to its original position hold still for about 1 second waiting until all the liquid is completely drawn in Fig C 2 Remove the tip slowly from the sample liquid If done slowly almost no liquid will remain on the outside surface of the tip If liquid does remain such as with sample liquids that have a strong viscosity wipe the tip w
11. tructure of the unit has been designed to reduce the effects of hand temperature on performance accuracy Our lineup of 5 different models allows a wide range of sampling between 1 0ul 1000ul lll Standard Accessories Tips x 3 Grease x 1 IV Safety Precautions Please be sure to carefully read the sections titled Safety Precautions and Cautions before using the instrument The contents of CAUTIONS are matters that require the user s attention not only for using the Nichiryo Le properly but for preventing the user from accidents and physical damage After reading this manual please keep it in a convenient place for easy referral at any time Please read the following prior to use for your safety and correct usage of the instrument 12 NCAUTIONS Be sure to observe the following instructions for using the NICHIRYO Le properly and safely Do not use this instrument for any purpose except pipetting liquid Do not modify this instrument as modification may cause an accident Do not use this instrument for pipetting any liquid to be injected into the human bogy Do not eject the tip towards anybody Do not discharge any sample liquid while pointing the instrument at anybody Some liquids are dangerous and may cause bodily harm Do not eject the tip while liquid is still inside Handle the tip with care as the point is sharp and dangerous Firmly attach the tip to th
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