MacroVue UVis-20 - GE Healthcare Life Sciences
Contents
1. nm lamps of the specified wattage Visually check that all five UV lamps or both white lamps are illumi nated If one or more lamps are burned out it is recommended that all lamps be replaced at the same time to ensure even light intensity To replace each lamp 1 Caution Turn the unit off and disconnect the power cord from the mains power 2 Remove the lamp cover by unscrewing the four retainer screws located on the sides of the unit 3 If present remove the tape that secures the lamps during shipping Remove each lamp by carefully rotating it about 1 4 turn counter clockwise until the pins align vertically Carefully pull the lamp upward 4 To insert a new lamp hold it so that the metal pins align vertically Place the lamp in the sockets at each end of the unit and lightly push it into place Rotate the tube 1 4 turn clockwise until it locks into place 5 Replace the cover and fasten the retainer screws hoefer macrovue uvis 20 user manual replacing fuses um 80 6304 39 p6 Replacing fuses Fuses protect equipment by disconnecting loads too large for the instru ment s circuit design For continued protection only replace with fuses of the specified voltage and current rating Each of two round modules on the left side of the control panel above the mains power cord receptacle holds one T 1A 250V 5 x 20 mm Slow blo fuse 1 Caution Set the power switch to off and detach the power cord before replaci2. 5 Rapid transfer of DNA from agarose gels to nylon membranes Nuc Acid Res 13 7207 7221 DNA nicking Vollrath D and R W Davis 1987 Resolution of DNA molecules greater than 5 megabases by contour clamped homogeneous electric fields Nuc Acid Res 15 7865 7876 Rapid site mapping Whittaker P A and E M Southern 1986 Ultraviolet irradiation of DNA A way of generating partial digests for rapid restriction site mapping Gene 41 129 134 Testing RecA function Maniatis T 1989 Maintenance and Testing of Lysogens of Bacterio phage Lambda in Molecular Cloning A Laboratory Manual eds Sambrook Fritsch and Maniatis 2 96 Ultraviolet sterilization Reich R R and Anderson H D 1986 Sterilization of Membrane Filters with Ultraviolet Radiation Pharmaceutical Manufacturing Jan Elimination of PCR contamination Wilson K H 1992 UV Absorption Complicates PCR Decontamina tion BioTechniques 13 208 210 Sarkar Gobinda and Sommer Steve S 1991 Parameters Affecting Susceptibility of PCR Contamination to UV Inactivation BioTechniques 10 590 594 Sarkar Gobinda and Sommer Steve S 1990 Shedding Light on PCR Contamination Nature 343 27 Yamada H and Hieda K 1992 Wavelength Dependence 150 290nm of the Formation of the Cyclobutane Dimer and the 6 4 Photoproduct of Thymine Photochemistry and Photobiology 55 541 548 e p7 hoefer macrovue uvis 20 user manual e customer ser
3. Q rana detection equipment MacroVue UVis 20 transilluminator Amersham um 80 6304 39 Rev C0 02 00 H e Biosciences Page finder Transilluminator function nennen 2 EL TE 3 Specifications eee aikkaankin kikk k kkkh kkk kki kkka kL EE EEEEEEEEEEEEE EEEn 3 LEIT Vru EL RE A Operating instructions 0 0 0 4 Care and maintenance ee ceeeeeceeeeeeteeeecteeeeetteeeentieeentieees 5 Troubleshooting 8 een nennen 6 UTC E 7 Customer service information nennen 8 hoefer macrovue uvis 20 user manual e uv and visible light transilluminator function MacroVue UVis 20 main components UV and visible light transilluminator function The Hoefer MacroVue UVis 20 combination UV and visible light transilluminator provides two back illuminated 20 x 20 cm work surfaces The left half of the viewing surface provides visible light for viewing and photographing visible stain gels and the right half provides a uniform and intense source of 302 nm ultraviolet UV light to back illuminate transparent fluorescent materials Nucleic acids stained with fluorescent dyes such as ethidium bromide and acridine orange can be detected under UV illumination This visualization method is especially suitable for double stranded nucleic acids but is less sensitive for visualizing single stranded nucleic acids The UV transillumi nator can be converted from 302 nm medium wavelen
4. Weight e 7 5 kg 15 5 Ibs Safety e All electric components are HYPOT tested to 1 500 V e The hinged cover blocks UV light hoefer macrovue uvis 20 user manual e important safety information A Note To protect the filter from cuts and corrosive buffers cover it with a protective surface such as the Hoefer UV transmitting work surface before placing the sample um 80 6304 39 p4 Important safety information e When working near UV radiation always wear at least a UV blocking face shield and cover all exposed skin For added eye protection wear goggles Make sure all persons in the area are also properly protected e Lower the UV blocking safety cover before switching the UV lamps on Always wear protective clothing even with the cover closed to prevent accidental exposure to UV radiation If the cover has been removed you must wear a safety shield and gloves to prevent damage to eyes and skin e If possible locate the transilluminator in a controlled environment such as a darkroom e Always disconnect the mains power cord before cleaning the unit or replacing fuses or lamps Operating instructions 1 Place the unit on a stable and level surface Allow space around the unit so that air can circulate freely 2 Plug the power cord into the receptacle at the back of the unit and into a properly grounded electrical outlet of the proper voltage rating The voltage rating of the unit is indicated on the label ab
5. gth to 254 nm short wavelength by replacing the UV lamps as described in the Care and Maintenance section Note The Hoefer MacroVue UVis 20 is equivalent to the Hoefer UVVis 20 Mighty Bright Double Vision Transilluminator Visible light viewing surface um 80 6304 39 p2 UV viewing surface Mains power switch A Dual control switch visible and ultraviolet light Hinged UV blocking safety cover e unpacking Unpacking Unwrap all packages carefully and compare contents with the packing list making sure all items arrived If any part is missing contact your local Amersham Biosciences sales office Inspect all components for damage that may have occurred while the unit was in transit If any part appears damaged contact the carrier immediately Be sure to keep all packing material for damage claims or to use should it become neces sary to return the unit Specifications Lamps e UV Five 8 W 302 nm UV e White light Two 8 W visible light Filter e The factory installed UV filter is for 254 nm and 302 nm lamps only Do not use with 365 nm lamps e The expected life of the UV filter is 5 OOO hours Note Swirl patterns in the UV filter if present do not interfere with viewing or photo documentation of gels Input fuses e 115 V T 1A 250V 5 x 20 mm 2 fuses e 230 V T 1A 250V 5 x 20 mm 2 fuses Dimensions h x dx w External with cover 14 3 x 33 7 x 48 6 cm 5 6 x 13 3 x 19 1 in
6. iosciences UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences Inc 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany www amershambiosciences com Amersham e Biosciences
7. ng fuses 2 Insert a small flat blade screwdriver into the slot on the fuse module and turn it 1 4 turn counterclockwise The spring loaded module cap will loosen Pull the cap fuse holder out 3 Pull the fuse out of its holder and inspect If the fuse element is burned or broken replace the fuse with an identical type If the fuse appears to be intact check it with an ohmmeter 4 Insert a good fuse into the holder and then insert this assembly back into the unit Seat the module by inserting the screwdriver into the slot pressing gently and turning the cap 1 4 turn clockwise Troubleshooting Unit does not operate when power is turned on e Check that the power cord is plugged into a working electrical outlet e Remove and inspect the fuses e Check that the lamps are properly installed Lamps flicker excessively e Check for proper line voltage Filter surface feels warmer than usual e Ensure that there is enough space around the unit so that air can circulate freely e bibliography Bibliography DNA crosslinking Church M C and G Gilbert 1984 Genomic sequencing Proc Natl Acad Sci USA 81 1991 1995 Khandjian E W 1987 Optimized hybridization of DNA blotted and fixed to nitrocellulose and nylon membranes BioTechnology 5 165 167 Khandjian E W 1986 UV crosslinking of RNA to nylon membrane enhances hybridization signals Molec Biol Report 11 107 115 Reed K C and D A Mann 198
8. ove the power cord receptacle 3 Place the sample on the selected filter Do not mar the filter surface by cutting gel on it or scraping gel away 4 To view the sample under White light Switch the dual control to the visible setting and then set the power switch to on I UV light Don goggles a UV blocking face shield and gloves Cover all exposed skin Lower the UV blocking safety cover Switch the dual control to the ultra violet setting and then set the power switch to on 1 After a momentary flick ering the UV lamps should be visible beneath the filter To remove the safety cover for photography lift the lid into the up position and pull out both inserts in the hinge assembly 5 After viewing the sample turn the unit off and clean according to the instructions in the care and maintenance section Caution Always turn off the transilluminator and disconnect the plug from the mains power before cleaning care and maintenance Care and maintenance Cleaning e Clean the filter surface after each use e Use a mild detergent and water on a soft cloth or sponge to clean the unit exterior e Never use abrasive cleaners solvents or chloroform on any part Replacing lamps The lamps in both sections of the transilluminator are replaced according to the same instructions The transilluminator is originally equipped with 302 nm UV lamps If 254 nm wavelength is required replace all UV lamps with 254
9. vice information um 80 6304 39 p8 Customer service information Technical service and repair Amersham Biosciences offers complete technical support for all our products If you have any questions about how to use this product or would like to arrange to repair it please call or fax your local Amer sham Biosciences representative Important Request a copy of the Amersham Biosciences Contami nation Clearance Certificate Form before returning the item No items can be accepted for servicing or return unless this form is properly completed Ordering information Quantity Code No MacroVue UVis 20 Transilluminator Dual 20 x 20 cm viewing surfaces 302 nm 8000 uW cm and cool white light Includes five 8 W UV lamps and two 8 W white lamps 115V 60 Hz 1 80 6225 16 230 V 50 Hz 1 80 6225 35 302 nm UV lamp 8 W 1 80 6223 26 254 nm UV lamp 8 W 1 80 6223 64 UV transmitting work surface 1 80 6224 21 Fuses for 115 V and 230 V models 5 80 6109 83 Hoefer and MacroVue are trademarks of Amersham Biosciences Limited or its subsidiaries Amersham and Amersham Biosciences is a trademark of Amersham plc 2000 Amersham Biosciences Inc All rights reserved All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group that supplies them A copy of these terms and conditions is available on request Printed in the USA Amersham B
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