MOXI Flow
Contents
1. Electrode short Previously used sensor D Used cassette detected o not reuse sensors Battery needs to be Use instrument with AC power or recharge instrument Low battery recharged for a minimum of 4 hours Instrument has Disk full a nn Delete histograms or download to computer storage capacity of 500 histograms After histogram is recorded and saved Disk now full there is no more space PEIE IES on the disk drive Internal firmware issue Clear error by turning instrument off and then on l due to instrument again Remove cell counter from sources of external Unhandled exception malfunction or high level interference If problem persists return instrument for of external interference service SPI timeout Instrument malfunction If problem persists return instrument for service Corrupt filesys Instrument malfunction If problem persists return instrument for service Instrument has detected corruption or new Defaults loaded version of firmware and If problem persists return instrument for service reset all settings to factory defaults False start Sensor malfunction Use new sensor False stop User touched the black Small Particle Mode lt 8 um box to start the test Re run test touching the main part of the start screen Reinsert the same cassette opposite side ora different cassette If problem persists return instrument for service Small Particle Mode selected Tests ca2. Moxi Flow applications can be entered from touching the Apps icon on the home screen Included applications in version 1 0 are System Is Ready Touch Assay Icon to Begin Clock e Programmable timer with sound notification e Restore restores unit to factory settings e Loader Puts unit is Firmware OS loader mode MA e Off powers the unit off Moxi Flow User Guide Page 13 Troubleshooting Symptom Corrective Action Questionable Concentration of Make sure concentration of cell or bead sample is concentration cell bead sample is too within recommended guidelines Refer to General high or too low Guidelines section Use a diluent that is compatible with cells being Wrong diluent counted ORFLO Diluent Cat No MXA006 or equivalent Ensure the cells are in a single cell suspension Break Cell clumping clumps by pipetting up and down with a standard pipette ORFLO recommends Accutase or equivalent Questionable cell diameter Use a diluent that is compatible with cells being Wrong diluent counted ORFLO Diluent Cat No MXA006 or equivalent Ensure the cells are in a single cell suspension Break Cell clumping clumps by pipetting up and down with a standard pipette ORFLO recommends Accutase or equivalent Try running the cell sample at a higher concentration u canon Refer to Managing the Data section for instructions on Peak of interest indistinct adjusting the Y axis Internal firmware issue du
3. warrants its products will meet their applicable specifications when used in accordance with their published instructions The ORFLO product warranty is a bumper to bumper warranty on your ORFLO automated cell analysis product for the term of the warranty This includes all electrical parts mechanical parts batteries displays materials and workmanship Our warranty also covers the materials and workmanship of our Cassettes and accessory products Please ensure that you have the latest version of the product user guides The most current version are found on our website at www orflo com How Long is Your Warranty Your ORFLO products are covered for a period of one 1 year from the original date of purchase from ORFLO or an approved sales distributor You may purchase for an additional fee an extended warranty for your Orflo cell analysis product for two 2 or four 4 years from the date of purchase The extended warranty extends the duration of your warranty coverage from the date of original purchase from ORFLO or an approved sales distributor but does not alter change or otherwise add to the coverage described here In accordance with EU requirements our standard product warranties remain in effect for two 2 years in EU countries What is Not Covered by Your Warranty The following exclusions apply to your ORFLO warranty e Damage resulting from misuse abuse or accidents e Damage resulting from failure to follow t
4. merchantability or fitness for a particular purpose The warranty provided herein and the data specifications and descriptions of ORFLO products appearing in ORFLO s product literature may not be altered except by expressed written agreement signed by an officer of ORFLO Technologies Representations oral or written which are inconsistent with this warranty or such publications are not authorized and if given should not be relied upon ORFLO Technologies shall not be liable for consequential incidental special or other damages resulting from economic loss or property damage sustained by any customer from the use of its products Moxi Flow User Guide Page 19 Moxi Flow User Guide Page 20
5. Upon power up the Moxi Flow will display a notification on the home screen that it is Exporting files to disk The Moxi Flow disk will be mounted to appear as a drive on the computer following completion of this file export process 5 Open the Moxi Flow drive folder and copy the files to your computer drag and drop or copy and paste Instrument Firmware and Software Upgrades This User Guide describes OS version 1 0 of the Moxi Flow firmware The firmware may be updated periodically For information on the most up to date firmware visit the ORFLO Technologies website at www orflo com Register your instrument in order to receive notification about relevant firmware upgrades Updating Moxi Flow Firmware via USB The Moxi Flow firmware can be updated through a USB connection For this method of update the user must connect the Moxi Flow to the computer s USB port via the provided USB cable Unplug the power adapter to expose the USB connector for the computer Next the user needs to put the Moxi Flow into a firmware update mode This can be done in either of the following two ways With the unit powered off quickly press the power reset button four times When successful the white Moxi Flow screen will display with text under the Moxi Flow logo that indicates the system is in firmware update mode If unsuccessful turn the power off and retry Moxi Flow User Guide Page 12 Moxi Flow Apps
6. mammalian cell lines but the appropriate dilution will depend on cell type and seeding density Type MF S cassettes will typically require no to 1 2 dilution depending on cell type and seeding density The volume required for an accurate count is approximately 50 uL Turn the instrument on by pressing the power button and the Home screen will be A xi displayed Select a test type by pressing Sa A one of the icons displayed on the screen 3 NOTE The instrument must be plugged in to an AC source to run atest Tests should not be run on battery power alone all OR Moxi Flow User Guide Page 7 3 Open the Upper and Lower Doors Insert a test cassette and close both Doors The Moxi Flow will automatically perform a calibration step and then prompt the user to load a sample when complete 4 Open the Upper Door and pipette a 50 uL sample into the fill port of the cassette either test 1 or test 2 depending on which end of the cassette was inserted into the instrument and close the Upper Door 5 The Moxi Flow will begin the test and the results will be complete in approximately 8 seconds The results will then automatically be displayed on the screen Managing the Data 1 The results of a test are initially displayed as a scatter plot The ae results of a Viability Count PI test are shown as an example Live cell and dead cell counts are displayed below the graph Live cell is reported in the yellow box a
7. O ORFLO M MOXI Flow USER GUIDE m a Sys fem is Head Va an a sa A ET Sul ope sare SORE Ato e 7 Al I r The information in this document is subject to change without notice and should not be construed as a commitment by ORFLO Technologies a DBA of E I Spectra LLC Neither Orflo Technologies nor any of its affiliated corporations assumes responsibility for any errors that may appear in this document This manual is believed to be complete and accurate at the time of publication In no event shall Orflo Technologies or any of its affiliated corporations be liable for incidental or consequential damages in connection with or arising from the use of this manual 2013 ORFLO Technologies a DBA of E I Spectra LLC All rights reserved Rev 1 0 0 06 2013 The ORFLO symbol and Moxi Flow are trademarks of E I Spectra LLC registered in the U S and other countries Windows Windows XP Windows Vista Windows 7 and Windows 8 are trademarks of Microsoft Corporation registered in the U S and other countries Mac and Mac OS are trademarks of Apple Corporation registered in the U S and other countries Bluetooth and the Bluetooth logos are registered trademarks of Bluetooth SIG Inc registered in the U S and other countries Accutase is a registered trademark of Innovative Cell Technologies TUV SUD and the T V SUD symbol are registered trademarks of TUV SUD Aktiengesellschaft Contents UOC WG OM seen ne e
8. bove the graph The gating can be manually adjusted by pressing the blue or red dots and dragging them to the desired position Live and dead cell counts will update automatically Viability Count PD Test 1 2 Press the Histo icon to view the results in the form of a histogram Press the up and down arrows to the right of the graph to increase or decrease the vertical scale Press the Dot Plot icon to return to the scatter plot 7 11 14 18 22 26 29 33 Moxi Flow User Guide 3 Press the blue square icon at the lower right corner of the graph to overlay histogram curves on the scatter plot Press the blue square icon again to remove the overlay curves 14 18 22 26 29 Diameter um Viability Count Pl 4 Press the Delete icon at any time to permanently delete the results of the test 3 7 1144 18 22 26 29 f 8 Diameter um Live Cells Dead Cells 1 3e 05 Cells ml 1 24e 04 Cells ml scree n 11 90 um dia 11 72 um dia J 5 Press the Done icon to save the results and return to the Home Moxi Flow User Guide Page 9 Retrieving and Deleting Data 1 To open a saved test press the Saved Data icon on the Home screen System Is Ready Touch Assay Icon to Begin Viability Apoptosis Count Pl Annexin V PE 2 Parameter Size Flow Cytometry Histogram NO 2 Icons for up to nine saved tests will be displayed on the screen Press the appropriate icon for the test of interest or press the Pag
9. e heat will damage the battery touch screen display and other electronic components The external surfaces of the Moxi Flow body and touch screen display can be sanitized by wiping with a soft nonabrasive cloth moistened with 70 isopropyl alcohol IPA or 70 ethanol Do not clean the instrument with any more aggressive solutions A CAUTION When sanitizing make certain that no liquid enters any internal cavities of the instrument This could result in damage the instrument Maintenance There is no routine maintenance required for the Moxi Flow In addition there are no user serviceable parts Instrument repairs must be carried out by authorized personnel only Moxi Flow User Guide Page 16 System Performance Detection Channels Laser Wavelength Number of PMT s Optical Detection Range Particle Size Detection Display Resolution Data Storage Connectivity Data Output Format Pre Programmed Tests Open Platform Printer Capable Cassette Performance Preferred Sample Type Effective Diameter Size Range Cell Volume fL Measurement Time Concentration Sample Volume uL Software Moxi Flow Firmware OS Dimensions Length Width Height Weight Electrical Specifications Internal Battery AC Power Adapters US and EU types Specifications for the Moxi Flow 2 1 color 1 size 532 nm 4 590 40 nm e g R PE Impedimetric Coulter Principle 480 x 320 colo
10. e re rue Bene T 1 ADOUL ING WISER GUC nee 1 Conventions Used in the User Guide sss sees 1 SAV md 2 7210 141 9 koe me ere tg ee ee ee 2 GENelal sale A eter eee See eee ee ea ee a eee ee eee eee ee 2 Bre RSs decays KS Venen 2 Biological Sale Nin n 3 Moxi Flow System Overview 4 MorF IOW ACCES SONES ee Geant tte tees ren ae Gea ee ee 5 Geld Slate ee ee 6 General GUIGSIINGS kei 6 MALEN ASIAN Cie ei reed 7 Using TS Mox FION u nenne 7 DOINGS rs Denen ee Beer re ea ee eh 7 RUMIN A OST ae es ae ee 7 Managing te Dana a a a a 8 Retrieving and Deleting Dafa 10 Moxi Flow Helper 11 Connecting to Moxi Flow via USB 12 Instrument Firmware and Software Upgrades e eee 12 Updating Moxi Flow Firmware via USB 12 Moxi Fow Te 13 TOUDIGSMOOUNG u ren 14 Mainlenancerand Storage ee elek ated G tant Aca cod cana tatoo 16 Specifications for the Moxi FIoWwTM 17 ordening FORMATION asian lese een 18 Technical SOIVIC 0 cc cccccccccccccececcccccccecececcacccececaceaeuacetaneaeueceteceaeaueceteneaeauscetseceausneraceceanens 18 Warranty Introduction The ORFLO Moxi Flow is a portable micro flow cytometer that utilizes a disposable thin film cassette and a combination of a 532nm laser precision optics analog and digital hardware and proprietary software to perform automated particle counting and analysis functions It combines the gold standard Coulter Principle with micro flow cytometry to deliver highly acc
11. e to instrument Reset instrument by pressing and holding the power Instrument stops responding malfunction or high level button for at least 5 seconds If problem persists return instrument for service of external interference Battery is faulty or has Battery will not fully charge surpassed its service life Return instrument for battery replacement Adjust pipette to ensure sufficient volume for the test Insufficient fluid lt 50 uL For improperly calibrated pipettes this might require a setting gt 50 uL Cells beads too large Make certain that the particle type is within the 2 specified size range of the cassettes being used Highly strongly aggregated cells clogging filter and blocking flow Try breaking apart cells using pipette trituration and or protease treatment Moxi Flow User Guide Page 14 Error Warning Messages Corrective Action en ap Make sure sample volume is at least 50 uL Pipette Lost start en more carefully to avoid causing bubbles Cassette not properly Do not remove cassette from Moxi Flow before Lost sensor detect inserted into cell completion of test cycle Ensure cassette is properly counter inserted Make sure concentration of cell or bead sample is Warning High Concentration Concentration of cell or bead sample is too high within recommended guidelines Refer to General Guidelines section Start open Stop open Issue detected upon Start stop short test cassette insertion
12. e w a tistos ap jest 16 Up or Page Down icon to view more test results Press the Done icon to close the test results and return to the saved results screen u GAD CHH 3 Press the Delete All icon to permanently delete the results of all saved tests Select a Histogram 4 Press the Close icon to return to the Home screen OMA Moxi Flow User Guide Page 10 Moxi Flow Help 1 For help with operation of the Moxi Flow press the Help icon on the Home screen Visual instructions for inserting a cassette will be S ystem Is Ready p rese nt e d Touch Assay Icon to Begin 2 Press the Next icon to view the next visual instruction that demonstrates how to pipette a sample into the cassette 3 Press the Next icon to proceed to the next instruction screen or press the Previous icon to return to the previous screen 4 Press the Done icon to exit the help screens and return to the Home screen For additional help see the Troubleshooting table page 12 Moxi Flow User Guide Page 11 Connecting to Moxi Flow via USB 1 Unplug the USB Cable from the Moxi Flow Power Adaptor 2 Plug the wide end of the USB Cable into your computer s USB port 3 Plug the small end of the cable into the back of the Moxi Flow 4 Make sure the Moxi Flow is powered on and wait for it to appear as a disk or flash drive in your computer s file system Windows Explorer for PC s or Finder for Mac Note
13. he proper operating instructions contained in the User Guide e Damage resulting from failure to use an ORFLO USB cable and power adapter Moxi Flow User Guide Page 18 e Damage resulting from unauthorized non ORFLO repair or improper tampering with the product e Damage resulting from fire flood or Acts of God beyond the control of ORFLO Technologies What To Do If You Think You Have a Warranty Issue Please contact ORFLO promptly at 1 855 TRY MOXI 855 879 6694 or send an email to info orflo com to describe the issue you are experiencing Please be prepared to supply your name contact information the serial number of your product and the original purchase date of the unit If your issue remains unresolved after consulting with one of our technicians ORFLO will provide a Returned Material Authorization RMA number and you will be asked to ship your product back to us at ORFLO Technologies Attn Warranty Returns RMA XXXX 130 Airport Circle Hailey ID 83333 An ORFLO technician will promptly examine your product to determine if the issue is covered under the ORFLO warranty If it is we will decide to either repair it or replace it at no cost to you including shipping Repair or replacement will be done as expediently as possible to ensure minimum disruption to your laboratory Express or Implied Warranties and Other Legal Stuff ORFLO Technologies makes no other warranty express or implied There is no warranty or
14. n only be rescaled immediately after the run If it is immediately after a run the user can cycle Incorrect x axis scale range waale through the scale ranges by pressing the scale range icon see Managing your data section Type S cassette run on Moxi Flow Firmware 3 5 Upgrade Moxi Flow system firmware to v3 6 or later or earlier Moxi Flow User Guide Page 15 Maintenance and Storage Storage Store the Moxi Flow and Moxi cassettes at room temperature in a dry environment For best results cassettes should be used within one year of purchase Avoid prolonged exposure to ultraviolet light as it may degrade the touch screen display or discolor the external surfaces of the instrument Charging the Battery The Moxi Flow contains a 3 7 V lithium ion battery which can be charged for approximately 500 cycles The battery may be charged at any time in the discharge cycle and can be charged continuously without damage using a PC Mac or the power adapter Refer to the Installation section for information on how to charge the battery A CAUTION To prevent battery damage use ONLY an Orflo supplied power adapter or the USB port of a computer The battery life is about 2 to 5 years depending on use Lithium ion batteries discharge even if they are not in use To prevent battery damage from self discharge charge the battery at least once every two months Cleaning Sanitizing The Moxi Flow is NOT autoclavable Extrem
15. nd run a test with the door open e Do not operate the Moxi Flow if there is any damage to the instrument s enclosure Moxi Flow User Guide Page 2 Biological Safety A WARNING Biological samples have the potential to transmit harmful or fatal disease The following guidelines should be followed to minimize the risk of exposure to biohazardous materials e Handle all biological samples as if they are capable of transmitting harmful infections e Wear appropriate personal protective equipment PPE including clothing eyewear and gloves e Do not pipette by mouth e Dispose of biological waste in accordance with all applicable local state and federal regulations Moxi Flow User Guide Page 3 Moxi Flow System Overview The Moxi Flow Kit includes the Moxi Flow instrument USB Cable Power Adapter US and EU versions only USB Flash Drive with User Guide and one pack of 25 Cassettes USB Cable Port Touch Screen Display Power Reset Button Lower Door Upper Door Test 2 Fill Port Test 1 Fill Port Disposable Test Cassette Moxi Flow User Guide Page 4 Component Touch Screen Display Power Reset Button Lower Door Upper Door USB Cable Port Cassette Function Allows user to interface with the instrument by pressing on icons and targets Displays all information needed for operation and analysis of results Turns instrument on and off Resets the unit when pressed and held for gt 5
16. nds that tests not be run on battery power alone General Guidelines The Moxi Flow system is used with Moxi Type MFM and Type MFS Cassettes Refer to the Specifications section for information on the operating ranges for the system The sample volume for a test should be 50 uL If necessary prepare dilutions of the sample using ORFLO Diluent Cat No MXA006 or a diluent that is compatible with the characteristics of the cells or beads The diluent used should not cause changes in the cell size and should have sufficient conductivity to enable operation of the instrument Water hypotonic or hypertonic solutions are not acceptable diluents Moxi Flow User Guide Page 6 Materials Required Cell or bead sample diluted and dissociated if necessary 50 uL minimum Pipette and appropriately sized pipette tips Using the Moxi Flow Settings Press the Power Button to turn on the instrument Set the date and time Nov 24 9 51 26 by pressing the Settings icon on the main menu of the Moxi Flow Then use the arrow keys and follow the instructions displayed on the screen Running a Test 1 If necessary dilute a cell or bead suspension with ORFLO Diluent or an appropriate diluent so that the cell concentration is within the operating range of the cassette being used Type MF M 1x10 to 1 75x10 cells mL Type MF S 1x10 to 2x10 beads mL For Type MF M cassettes a dilution of 1 5 to 1 20 is recommended for most
17. r touchscreen 1200 histogram bins 2 Gb full histograms and scatter plot data USB on the go FCS 3 1 Viability Apoptosis CD4 T Cell Size Histogram PMT Histogram MOXI PLEX Bead Assays multiplexed Yes 2 Parameter requires R PE reporter Yes wireless Bluetooth printing Type MFS Type MFM Beads Cell Preparations 3 15um 4 20um 14 1800 34 4200 8 seconds 5 seconds 1x10 2x10 beads mL 1x10 1 75x10 cells mL 50 50 PC or Mac compatible requires Windows XP Windows Vista Windows 7 or Mac OS X operating system v 10 6 or greater Version 1 0 8 0 in 20 3 cm 4 5 in 11 4 cm 4 5 in 11 4 cm 2 75 Ibs 680 g Rechargeable 3 7 V 4500 mAh lithium ion Input 100 240 VAC 50 60 Hz 0 2 A Output 5 VDC 1A Operating Environmental Conditions Temperature Maximum Relative Humidity Moxi Flow User Guide 15 30 C 20 80 non condensing Page 17 Ordering Information This section lists catalog numbers for the Moxi Flow Automated Cell Counter and related products You can purchase Orflo products through a regional distributor or on line at www orflo com See the Technical Assistance section for information about contacting Orflo Product Description Cat No Quantity Pack Technical Service For technical service contact ORFLO Technologies at 855 TRY MOXI 855 879 6694 or email us at info orflo com Warranty What is Covered By Your Warranty ORFLO Technologies ORFLO
18. seconds Manually opened and closed by user to allow insertion of a test cassette Manually opened and closed by user to allow loading of a test sample Door must be closed prior to running a test Connects instrument to USB cable Disposable used for loading samples Each cassette contains two fill ports thereby allowing for two tests to be run per cassette Moxi Flow Accessories Power Adapter US style Power Adapter EU style USB Cable Moxi Flow User Guide USB Flash Drive with User Manual Page 5 Part Function USB Cable Connects instrument to PC Mac or power adapter Power Adapter US Style Connects USB cable to a 110 VAC outlet US kit only Power Adaptor EU Style Connects USB cable to a 220 VAC outlet EU kit only USB Flash Drive Contains Moxi Flow User Guide Getting Started The Moxi Flow is shipped in a condition ready for initial use To begin plug the USB Cable into the instrument at one end mini USB Plug the opposite end of the USB Cable standard USB into the Power Adapter Plug the Power Adapter into an AC outlet that matches the rating of the Power Adapter A CAUTION Use only an Orflo supplied USB Cable and Power Adapter Use of other products may result in inaccurate test results or damage to the instrument NOTE Tests should only be performed with the instrument powered by an AC source Although the instrument has an internal rechargeable battery Orflo strongly recomme
19. t may be helpful to the user Moxi Flow User Guide Page 1 Safety Precautions The Moxi Flow has integrated safety features that are designed for the protection of the user Use the Moxi Flow only as directed in this guide Please review and understand the safety instructions below before operating the system General Safety A WARNING To avoid the danger of electric shock e Prior to use verify that the USB cable and USB charging adapter are plugged securely into a properly grounded AC power outlet Verify that the connection between the USB cable and the instrument is secure and the AC power in your location is within the specifications for the instrument see page 17 e Do not immerse the instrument USB cable or USB power adapter in liquid or allow liquid to enter the instrument e Do not attempt to disassemble or service the Moxi Flow The instrument has no user serviceable parts All service must be performed by ORFLO Technologies A WARNING To avoid the danger of fire or explosion do not use the Moxi Flow with flammable or explosive liquids Laser Safety A WARNING The Moxi Flow is a Class laser product The system contains a Class IIIb laser that operates at 532 nm Direct exposure to laser radiation is harmful The following guidelines should be followed to avoid exposure e An interlock on the upper door prevents the laser from operating when the door is open Do not attempt to defeat the interlock a
20. urate and repeatable results in less than 10 seconds per test The Moxi Flow performs very accurate cell counts and quantitative assessments of cell health apoptosis and cell cycle The system is portable making it ideal for use in a hood or multiple lab locations Test results are displayed in the form of histograms and scatter plots The Moxi Flow can store up to 2GB of data and if desired the data may be downloaded to a PC or Mac via USB connectivity via a Bluetooth wireless connection The system is intended for research use only and has been tested with cell types that are representative of those commonly used It is not intended for use in medical diagnostic procedures About the User Guide The Moxi Flow User Guide provides detailed information for operating maintaining and troubleshooting the Moxi Flow system The user guide does not include instructions for using the optional Moxi Flow Analysis Software For more information on this product please visit www orflo com Similarly the user guide does not include information on preparing samples prior to running them in the Moxi Flow For more information on this topic please visit www orflo com Conventions Used in the User Guide A WARNING _ Alerts you to a situation that may cause injury to the user A CAUTION Alerts you to a situation that may cause damage to the system loss of data or incorrect results NOTE Indicates additional related information tha
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