User Manual
Contents
1. 1 Select the File name to annotate and press the Notes box above the data window The alpha numeric keyboard will appear Homa MWe dite 2 Enter your comments using the keyboard and then press the Save button Your comments in the Notes box is be attached to the file and shown on all Data tabs i e Layers Zoom and Analysis Press the Close button to return to the previous screen without your comment 2014 NanoEntek Inc Arthur Image based cytometer 33 Operation Data Horma Me opt es Export data FI F3E3ET g s E3EAE3E3ET Ee uw TE Li priam TER 7 i EE dim d Analisis Data table contains comprehensive information about the count with size and fluorescence intensity of each individual particle cell in the sample and provided in csv file and fcs file format Images exports a single image in each channel collected for each field of view Images are exported as jpg files Report contains only the aggregate results and calculations from the run such as the total cell count and concentration counts and concentrations of fluorescent cells and their relative abundance in the sample thumbnails of the bright field image of each field of view captured as well as the relevant histograms and instrument settings in a pdf file page 36 34 Operation Data 1 Connect the Arthur USB drive or any other USB drive2. 1 SET Arthur us Cell analysis slide 50 slides box 100 counts For used with Arthur Image based cytometer Arthur NanoEnTek NanoEnTek www nanoentek com BN USB drive Calibration beads 4GB Green Cali Beads Red Cali Beads and TFA Alignment Beads 1 KIT Arthur g Image based cytometer User manual Ouick manual 1 EA 1 EA User Manual Ouick Manual 1 2 3 Product overview The Arthur Image based cytometer is a 3 channel bright field green fluorescence red fluorescence desktop image analyzer that allows users to perform assays for cells in suspension including GFP and RFP expression apoptosis cell viability live dead and total cells cell cycle and cell counting assays using state of the art optics It is compatible with a wide variety of eukaryotic cells Depending on complexity of the assay and number of fields captured it takes 10 seconds to 2 minutes that Arthur counts for a typical assay with only 25 uL of sample volume In addition to the bright field channel the Arthur features two fluorescent channels green and red enabling it to simultaneously count green or red fluorescent stains as well as cells expressing GFP and RFP The Arthur offers an intuitive user interface and provides the option to save data and generate a report which can then be transferred to a PC using the USB drive supplied with the instrument or av
3. is preset in order to perform accurate cell counting for the majority of cultured cell types without changing default counting parameter The global parameters for counting described below Set counting settings Sensitivity refers to the contrast of the objects from the background Increasing the sensitivity makes the instrument more sensitive to objects in the bright field while decreasing the sensitivity makes the instrument less sensitive which is useful if there is a significant amount of debris in the sample Circularity is used to determine which objects to include in the measurement based on their roundness Increasing the circularity value requires objects to be rounder for inclusion in the measurement and may be useful if you need to distinguish perfectly round cells from more oddly shaped cells Decreasing the circularity allows objects to be less round for inclusion in the measurement and may be useful if the cell type is not particularly circular or perhaps oddly shaped Counting settings Sensitivity Increased sensitivity increases recognition of fainter objects in bright field Circularity Higher circulanty requirement recognizes more circular objects as cells 2 Change sensitivity and circularity by moving slider If necessary to return to default values press the Default button zs n addition to sensitivity and circularity you can also adjust the parameters for the cell size and green and or red fluore
4. Assurez vous toujours que la tension d entree d alimentation correspond a la tension disponible dans votre endroit Cetinstrument est refroidi a l air de sorte que ses surfaces peuvent devenir chaudes pendant le fonctionnement Lors de l installation de l instrument laisser un espace de plus de 10 cm 4 pouces autour de cet instrument et ne placez aucun objet entre l appareil et le mur N installez pas l instrument sur une pente ou un endroit soumis a des vibrations ce qui induit le risque de dysfonctionnement ou d endommagement de l instrument N inserez jamais aucun objet dans les orifices d aeration de l instrument car cela pourrait entrainer un choc electrique des blessures chez les utilisateurs et des dommages d equipement Branchez le cordon d alimentation fermement dans la prise murale et l adaptateur secteur aussi Pour eviter un risque potentiel de commotion electrique assurez vous que le cordon d alimentation est correctement mis a la terre Assurez vous de positionner l instrument de telle sorte qu il soit facile de debrancher l instrument Eteignez l instrument avant de debrancher le cordon d alimentation et ou de deplacer l instrument Sil instrument est casse ou qu il soit tombe debranchez le cordon d alimentation et contactez un technicien de service Ne demontez pas l instrument et la garantie sera annulee en cas de demontage Utilisez uniquement les accessoires autorises l adaptateur l
5. into the Arthur Export data 2 Select the Data file to export Press the Select all to highlight all files Select all 3 Press the Data table csv and fcs button Image jpg button or Report pdf button to export the data in the designated formats Press the All formats button to export data in all these formats Home Ma maine 4 Transfer the files on the USB drive to your PC You may open the exported files using the appropriate programs 2014 NanoEntek Inc Arthur Image based cytometer 35 Operation Data The image below shows an example of a data analysis report exported as a pdf Cell analysis report A rt h LI r Cell analysis report Image based cylpmeter Fia rose APP CAM HET BED Ruf 8 Emre Les aci CES 15 12 1 sta Arka m i d Hie ba panel ammi egit Curiae vy Lira Deeg Aprire D D 6 Monte 1 37 K How cari om tu em LEJR 8 US ka 8 858 end crdi Hr Asa cli ue Len loai celi pnr 1X Idi pn B felis captured Notes 36 Cleaning amp Maintenance Clean the surface of the Arthur with a damp cloth To clean the LCD screen turn off Arthur disconnect the power cable and clean the LCD screen with a soft cloth lightly moistened with LCD cleansing detergent Cleaning the screen with excessive force can damage the LCD screen Wipe the screen dry immediately Do not reuse the cell count
6. 1x 10 1x 10 cells mL 3 channels bright field green fluorescence red fluorescence 5 40 C Green channel LED 458 20 nm Red channel LED 530 20 nm Green channel 466 40 EX 495 LP Di 525 50 EM Red channel 543 22 EX 580 LP Di 585 LP EM 1 3 Mega pixels 4x objective 4x or 16x digital zoom 4 GB 290 mm W x 440 mm D x 290 mm H 8 7 kg Polymethy methacrylate PMMA 110 mm W x 24 mm D x 1 9 mm H 25 uL 42 Ordering information Cat No AT1000 ACOO050 ACO500 ABO100 Description Arthur Image based cytometer Arthur Cell analysis slide 50 slides Arthur Cell analysis slide 500 slides Arthur Calibration beads 100 uL O 2014 NanoEntek Inc Arthur Image based cytometer 43 Technical support Visit the our Website at www nanoentek com for Technical resources including manuals FAQs etc R I Technical support contact information Additional product information and special offers For more information or technical assistance please call or email NanoEnTek Inc HQ 5 Digital ro 26 gil Guro gu Seoul 152 740 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 NanoEnTek USA Inc 5627 Stoneridge Drive Suite 304 Pleasanton CA 94588 USA Tel 1 925 225 0108 1 888 988 0108 Toll free Fax 1 925 225 0109 Email sales nanoentek com Website www nanoentek com 44 All the materials in this user guide are protected by Korean and internati
7. Sina Ltpes F ni 4 If necessary to run the next sample insert the new slide and repeat the procedure 24 Operation Running sample Review captured image 1 Press the thumbnail of the image displayed in the Zoom tab to select a captured field of view for review Zoom TES edm LR ELI piem 3 118 ES ma cram Ster giae m amo E aee HE CE LLL LT LS 2 Move the Green square in the slider bar to zoom in and out the image EIE AX 18x 3 Press the image to display the navigation tool for reviewing a different section of an image viewed at 4x or 16x magnification 4 Select the appropriate area on navigation tool to display the desired section of the image 5 Press the image outside the navigation tool to hide the tool and review the image of images to capture 8 holds k 5 of mages 1o captum Zoom Layers w The navigation tool is only available for reviewing the images at 4x and 16x magnification O 2014 NanoEntek Inc Arthur Image based cytometer 25 Operation Running sample Layers 1 Press the thumbnail of the image and the Layers tab to review images captured through the bright field the green fluorescence and the red fluorescence Dain Press Lo eet res 5 E amm pp ES voies LAE TL I od i 0 CT IT S ums ga eye Hd e iram
8. blue lines on the histograms represent the set thresholds 1 Press the cell size thumbnail to set the gating parameters for cell size Call Size Green 2 The Cell size vs of cells histogram will appear Move the two green square buttons on the slider bar to set the minimum cell size and maximum cell size Homa Pain Sattingt Cell size Sample 3 Press the Apply button to confirm and return to the previous screen Apply 2014 NanoEntek Inc Arthur Image based cytometer 27 Operation Running sample Gating 4 Press the Green histogram thumbnail to set the threshold for the Green fluorescence Home Dala Dar 1 ui LC Fier ts ee R S Cata E I Layers 5 Press the Sample fluorescence button or the Control fluorescence button on the bottom left 6 Press on the minimum or maximum RFU value relative fluorescence unit to set the x axis scale and type in a new value using the popup alpha numeric key pad 7 Move the arrow button on the slider bar to set the threshold Press the RFU threshold button and type in the threshold value using the alpha numeric popup key pad 8 Press the Apply button to confirm Or press the Close button to return to the previous screen without setting Apply 9 Press the Red histogram thumbnail to set the threshold for Red fluorescence N IMPORTANT When using the Arthur Cell Cycle Assay the Propidium lodide PI hi
9. c sidi 8 TIN nen ca Layers 7 The number of layers that are available for viewing and the labels for those layers depend on the Arthur Assay performed In the example above the buttons for the fluorescent channels are labeled Green and Red because the Arthur Green Red Assay is selected However if the Arthur Apoptosis Assay was selected these same buttons would be labeled Annexin V for the green channel and PI for the red channel 2 Press the Bright field Green Red buttons as necessary When multiple layers are turned on they appear overlaid on the image 3 Press the Circle button to identify the cells counted through a particular channel Arthur circles the cells that were analyzed as follows amp af images to capture Blue cells counted in bright field channel Green cells counted in green fluorescence channel Red cells counted in red fluorescence channel Yellow cells counted in both green and red channels Black cells excluded from the counting results 26 Operation Running sample Gating i The procedure for setting gates and thresholds is identical for all Arthur Assays but the buttons on the touch screen are labeled according to the specific assay selected For example the histogram thumbnails that are labeled Cell size Green and Red for the Arthur Green Red Assay see image below are labeled Cell size Annexin V and PI for the Arthur Apoptosis Assay The thin
10. care to avoid forming bubbles in the sample The Arthur counts the cells in the chamber opposite the inserting direction For example to count the sample in Chamber A you need to insert the Arthur Cell analysis slide into the instrument with the Chamber B side first until it stops For inserting the Arthur Cell analysis slide into the instrument The Arthur Cell analysis slides are specifically designed for use with the Arthur exclusively Use of other slides result in inaccurate cell counts and can damage the Arthur 20 Operation Loading sample 2 The Arthur counts the cells in the chamber opposite inserting direction T Inserting direction Sample loaded N IMPORTANT To count the sample in Chamber A insert the Arthur Cell analysis slide into the slide port of the instrument with the Chamber B side first until it stops 3 Press the Press to insert new sample button the slide will automatically be pulled into the instrument Press lo insert new sample zs f you are performing a background measurement the button for inserting the sample will read Press to insert unstained control page 23 2014 NanoEntek Inc Arthur Image based cytometer 21 Operation Focusing 1 Adjust the focus using the Focus knob on the right side of the instrument 2 Press the Zoom button and select either 4x or 16x magnification enhancement to review the image Correctly focused images hav
11. viability count results ensure the counting area is covered with cell suspension and count cells immediately after staining per the assay protocol Re calibrate the Arthur after updating the firmware or when using assay reagents not formulated for the instrument page 13 The internal working memory of the Arthur is 145 Gigabytes sufficient for storing comprehensive data from 500 sample runs However we recommend that you save your data to the USB drive after completing each experiment Using the USB drive you may then transfer the data to your PC as described in Export data page 34 After using Arthur Cell analysis slides appropriately dispose of them as biohazardous waste Do not reuse the Arthur Cell analysis slides Turn off the Arthur at the end of the day O 2014 NanoEntek Inc Arthur Image based cytometer 17 Operation Selecting assay 1 To select a Arthur assay press the appropriate assay button Quick count Cell Health Cell Cycle or Green Red on the Home screen For example press the Cell Health button for the viability and apoptosis assay options Mesure ENIN seltings Quick eati Get iHmallh Coll Cycia ienn Red Please choose assay 2 Next Apoptosis assay is selected from the options giving you the option to name the sample now or later Mesure ENIN stllin Quick ecani Cep Haalth Gali Cyrin enh i Rad Annexn V Name sample senes Operation Naming th
12. 6 Arthur Image based cytometer User Manual Enlek Contents Product Components Product Overview Description Installation Arthur Operation Settings Set date amp time Set counting settings Align cameras Calibrate Update firmware Recommend actions Selecting assay Naming the sample Loading sample Focusing Measuring background optional Running sample Capture Review captured image e Layers Gating Result e Viability Apoptosis Cell cycling Green fluorescence 9 36 9 16 10 11 12 13 15 16 17 18 19 20 21 22 23 24 28 24 25 26 27 28 29 30 29 29 30 30 Data Select data file Rename Delete e Review Annotate Export data e Cell analysis report Cleaning amp Maintenance Warranty Safety precautions Product specifications Ordering information Technical support 31 36 31 32 32 33 33 34 35 36 37 38 39 41 42 43 44 45 O 2014 NanoEntek Inc Arthur Image based cytometer 3 Product components Arthur is shipped with the following components Please check all components listed below when an instrument was delivered If any item is missing or damaged contact your local distributor or e mail sales nanoentek com B W 1L WB Image based cytometer Power cord 4 pcs set for U S Canada Cell analysis slide C D Taiwan Japan Europe or UK Cat No ACO050 1 Box 50 slides box
13. For your protection Arthur units being returned must be insured against possible damage or loss Nanoentek cannot be responsible for damage incurred during shipment of a defective instrument It is recommend that you save the original packing material in which the instrument was shipped This warranty is limited to the replacement of defective products For any inquiry or request for repair service contact sales nanoentek com or your local distributor 38 Safety precautions Review and follow the safety instructions below f water or other material enters the instrument adaptor or power inlet disconnect the power cord and contact a service person For operating environment refer to Product Specifications page 42 Do not touch the main plug or power cord with wet hands Always ensure that the power supply input voltage matches the voltage available at your location This instrument is air cooled and its surfaces may become hot during operation During installing leave a space of more than 10 cm 4 inches around the instrument and do not place any objects between the instrument and the walls Do not install the instrument on a slant or a place prone to vibrations which induces the risk of instrument malfunction or damage of the instrument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord f
14. ailable separately The Arthur is supplied with disposable Arthur Cell analysis slide which are also available separately Features Is designed for compact desktop design for fast easy to use and accurate 3 channel assay Uses disposable Arthur Cell analysis slide that eliminate washing steps and cross contamination between samples Provides comprehensive test results with graphic data and the cell analysis report allowing the export of data as csv comma separated value jpg fcs and pdf files for archiving and sample comparisons O 2014 NanoEntek Inc Arthur Image based cytometer 5 Description Front View Power button The power button is used to turn the instrument on and off The blue status light indicates that the instrument is on LCD touch screen The LCD touch screen display located in the front of the instrument provides buttons for all the functions available and shows data from the cell count Slide port The slide port is used to insert the Arthur Cell analysis slide containing the sample into the counter for analysis USB port The USB port allows you to transfer and save the cell count data and image to your computer for record keeping and printing purposes The USB drive supplied with the instrument or any other standard USB drive is inserted into the USB port for data transfer Focus knob The focus knob is used to adjust the image guality to obtain better contrast between the cells and t
15. e sample 1 Press the Name now button to name the sample before performing the assay The alpha numeric keyboard appears 2 Type the name of the sample series using up to 40 alpha numeric characters and then press the Save button Each sample run in the series will be appended with a number to reflect the order in which it was run Press the Close button to return to the previous screen without naming the sample 7 f pressing the Name later button the name for each sample series by date and time will be automatically assigned Go to the Data menu to rename if necessary page 32 O 2014 NanoEntek Inc Arthur Image based cytometer 19 Operation Loading sample 1 Load 25 uL of your sample per slide chamber on the Arthur Cell analysis slide using pipette as described below Make sure to mix the sample thoroughly before pipeting Load the sample gently to spread it evenly in the cell analysis slide 2 lli N IMPORTANT Follow the guidelines below to load your samples on the Arthur Cell analysis slide Do not touch the optical surfaces of the Arthur Cell analysis slides Hold the slides by the edges Use 25 uL of sample volume per slide chamber Do not overfill or underfill the slide chambers Pipette the sample at an angle of approximately 80 into the half moon shaped sample loading area The sample is loaded into the chamber through capillary action Take
16. e cordon d alimentation et le lecteur USB A Warning Le produit de classe A est concu pour l utilisation dans un environnement industriel Dans la documentation de l utilisateur la d claration doit tre incluse pour attirer l attention sur le fait qu il peut y avoir des difficult s potentielles pour assurer la compatibilit lectromagn tique dans d autres environnements en raison des perturbations rayonn es et conduites par l lectricit 40 Safety precautions Review and follow the safety instruction below Symbol Meaning Protective earth Ground AN Caution amp Warning C 6 This instrument and consumables conforms to the EC Declaration of Conformity This eguipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the eguipment is operated in a commercial environment FCC Compliance This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Disposal of your old appliance1 When this crossed out wheeled bin symbol is attached to a pr
17. e uniformly dark colored cells surrounded by bright halos see example on the left below Cells may be undercounted when the transition between the background and the edges of the cells are fuzzy and the cells have undefined boundaries see example in the middle below Cells maybe overcounted when they have bright centers and dark perimeters see example on the right below Image is correctly focused Incorrect undercounted cells Incorrect overcounted cells Cell type Hep3B 22 Operation Measuring 1 Press the Background tab to see screen opens optional Cama Wf DAS ee ek ber PUR pump rbl Leben 44 EE The Background feature is available for Arthur Viability Apoptosis Green Red and Cell Cycle assays except for the Quick count assay which does not use fluorescent reagents x No Background will be displayed on the dropdown menu if there are the saved background measurements for the assay on the instrument The fluorescence threshold which you can change manually after the assay will be set up when choosing not to run a background control In use No Background EA lt The latest background will be displayed on the dropdown menu it there are the saved background measurements on the instrument Inuse LIVE DEAD 00 am 2 Select the File name from the drop down menu to use a previous background measurement If you want to change a previously obtained backgr
18. ect Multiple button to select more than one file at a time The Select Multiple button will be disabled when pressing it again Select All button to select all files in the list w The internal working memory of the Arthur is 145 Gigabytes sufficient for storing com prehensive data from 500 sample runs However we recommend that you save your data to the USB drive after completing each experiment Using the USB drive you may then transfer the data to your PC as described in Export Data page 34 O 2014 NanoEntek Inc Arthur Image based cytometer 31 Operation Data Rename Data Delete 1 Select the Data file to rename 2 Press the Rename button to rename the selected files The alpha numeric keyboard will appear Harne satinga Rename 3 Enter the file name using the keyboard and then press the Save button Press the Close button to return to the previous screen without renaming 1 Select the Data file s to be deleted 7 Select Multiple button to select more than one file at a time The Select Multiple button will be disabled when pressing it again Select All button to select all files in the list 2 Press the Delete button to delete the selected file s 32 Operation Data Review Data Annotate 1 Select the File name from the Data menu to review the saved data 2 Press Layers Zoom or Analysis tabs to display the corresponding screens
19. ert the slide with calibration beads into the slide port for instrument and press the Insert sample button Insert sample 9 Adjust the focus using the Focus knob 10 Press the Run button to start the auto calibration of the red channel Operation Settings 11 Press the OK button to return to the Settings menu Arthur does not require Calibrate recalibration each time the instrument is turned on Measure Finish calibration N IMPORTANT Insert the slide with calibration beads just at the time of use to prevent drying of solution in slide Allow the beads to settle in the slide before pressing run There should be movement of beads on the image The entire calibration can take up to 20 minutes to complete 2014 NanoEntek Inc Arthur Image based cytometer 15 Operation Settings 1 Periodically Nanoentek adds functionality and other improvements to Arthur user Update firmware interface We recommend keeping your Arthur update with the latest firmware using the Update buttons If you have any questions about the software updates contact sales nanoentek com 2 Visit our website at www nanoentek com to download update software which will be supplied as a zip file to your desktop 3 Unzip the Arthur Update zip file and save the software in a USB drive One folder one ini two exe six dll IMPORTANT The files must reside individually on the USB dr
20. f the Arthur is to set the dynamic Calibrate range of the instrument The calibration procedure is most effective when performed after alignment seguence 1 Press the Calibrate Green Red button on the Setting menu The Calibration dialog will appear Floris Calibrate Green Red Load green cattwalion beads 2 Load 25 uL of Arthur Green calibration beads on the Arthur Cell analysis slide Make sure that the beads are mixed thoroughly before pipetting page 20 zs Make sure to mix the bead thoroughly before pipeting Load the bead gently to spread it evenly in the cell analysis slide 3 Insert the slide with calibration beads into the slide port of instrument and press the Insert sample button Insert sample 4 Adjust the focus using the Focus knob Homa Maatua Focus your sampla using tha focus knob kob Correct focus Incorrect focus 2014 NanoEntek Inc Arthur Image based cytometer 13 Operation Settings 5 Press the Run button to start the auto calibration of the green channel Calibrate Run 6 When the green channel calibration is complete the cell analysis slide will be ejected automatically and the red channel calibration dialog will appear Flames TEE Load green cakbralion beads 7 Load 25 uL of Arthur Red Calibration Beads on the Arthur Cell analysis slide page 20 Make sure that the beads are mixed thoroughly before pipetting 8 Ins
21. he background as wellas between cells that have taken up Arthur reagents and unstained cells Power button USB port Slide port Focus knob LCD touch screen a Description Rear and Side view Focus knob Power inlet On Off switch The power inlet connects the instrument to an electrical outlet through the supplied power cord and the appropriate plug based on the electrical outlet configuration in your country The on off switch is the main power switch that is not re quired to use for day by day operation of the Arthur On Off switch Power inlet O 2014 NanoEntek Inc Arthur Image based cytometer 7 Installation Installing Arthur 1 After unpacking the instrument place the instrument on a flat level dry surface 2 Plug one end of the supplied power cord into the Arthur To the other end attach the appropriate plug adaptor based on the electrical outlet configuration in your country 3 Plug the power cord into the electrical outlet Be sure to use only the power cord supplied with your instrument Powering the instrument with an unapproved power cord may damage the instrument 4 Turn on the main power switch i e On Off switch located above the power inlet port page7 5 When you are ready to use turn on the Arthur by pressing the Power button on the front of the instrument 6 When the Arthur is turned on the instrument starts up and d
22. ing slides Arthur does not need regular maintenance To troubleshoot problems with Arthur contact Technical Support page 44 IMPORTANT Never disassemble or service Arthur by yourself Unauthorized repairs may damage Arthur or alter its functionality which will void your warranty Contact sales nanoentek com or your local Arthur distributor to arrange for service Always wipe surfaces with ethanol soaked paper towels Do not directly spray ethanol anywhere on Arthur Avoid exposing Arthur to UV light UV light may degrade components including plastic Damage from UV exposure is not covered under the manufacturer s warranty O 2014 NanoEntek Inc Arthur Image based cytometer 37 Warranty Nanoentek warrants the Arthur will be free from defects in material and workmanship for a period of one 1 year from date of purchase Ifany defects occur in Arthur during this warranty period NanoEnTek will repair or replace the defective parts at its discretion without charge The following defects however are specifically excluded 1 Defects caused by improper operation 2 Repair or modification done by anyone other than Nanoentek or an authorized agent 3 Damage caused by substituting alternative parts 4 Use of fittings or spare parts supplied by anyone other than Nanoentek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample
23. irmly into the wall outlet and AC adapter To avoid potential shock hazard make sure that the power cord is properly grounded Be sure to position the equipment such that it is easy to disconnect Turn off the instrument before unplugging the power cord and or moving the instrument If the instrument is dropped or broken disconnect the power cord and contact a service person Disassembly of case will void warranty Use only authorized accessories adaptor power cord and USB drive Ab Warning Class equipment is intended for use in an industrial environment In the documentation for the user a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances O 2014 NanoEntek Inc Arthur Image based cytometer 39 Consignes de securite Examinez et suivez les consignes de securite ci dessous N installez pas l instrument dans un endroit humide comme une serre ou un incubateur pour eviter un risque de choc electrique Si de l eau ou tout autre materiau penetre dans l instrument l adaptateur ou l entrees d alimentation debranchez le cordon d alimentation et contactez un technicien de service Pour l environnement d exploitation reportez vous aux specifications du produit Netouchez pas la fiche ou le cordon d alimentation principale avec les mains mouillees
24. isplays the Home screen From the Home screen you can proceed immediately to Arthur Assays functions including Quick count Cell Health Cell Cycle or Green Red and you can use the naviga tion bars to access saved data Data screen or adjust instrument settings Settings screen kliegt Setting Chuck pour Cal Grenn d Rei Arthur Image based cytometer IMPORTANT When installing or moving Arthur it is recommended to perform auto alignment page 11 Operation 1 Press the Settings menu on the navigation bar to see the Settings screen Settings Hone Measure Data Settings Ferrero satira Scromn noltings The Settings screen allows you to Calibrate and align the instrument to ensure optimal performance page 13 Update to install new firmware version as it become available page 16 Set up date and time see below Adjust counting settings sensitivity and circularity page 10 Settings 1 The date and time is preset Press the Settings menu to reset date and time based on Set date amp time your environment 2 Choose the date and time by moving up and down the roller wheel After completing date time setting the updated date time will be displayed on the top right corner of the screen screen settings 2014 NanoEntek Inc Arthur Image based cytometer 9 Operation Settings 1 Press the Settings menu to adjust counting setting The counting parameter of Arthur
25. ive Do not transfer as a folder or put the files in a folder 4 Connect the Arthur USB drive to main body of the Arthur 5 Check if the version of software and firmware are the latest one Press the Update firmware button The update process takes a few seconds 6 The Update dialog will appear Press the Update button to start updating Home ETC Dala ry Sees 110577 PE am ee IDE SET Firmware salti ining 5018 Update To update firmware insert USB drive Latin latest maare files All saved data tram i a r ra d instrument val remain in memory 7 After updating press the OK button to restart the Arthur automatically Operation Recommend actions To obtain the best results follow these recommendations Wear gloves during sample handling Do not touch the optical surfaces of the Arthur Cell analysis slides Hold the slides by the edges Use the Arthur at room temperature only 5 40 C The Arthur contains delicate optics Place the instrument on a flat dry surface that is free from excessive vibration Do not spill liquids on the surface of the instrument or introduce liquids into its interior The Arthur can accurately count cells 5 um to 60 um in diameter The recommended sample concentration range for the Arthur is 1 x 10 to 1 x 10 cells mL however the sample concentration does not need to be exact to perform an assay For accurate
26. oduct it means the product is covered by the European Directive 2012 19 EU 2 AII electrical and electronic products should be disposed of separately from the municipal waste stream via designated collection facilities appointed by the government or the local authorities 3 The correct disposal of your old appliance will help prevent potential negative consequences for the environment and human health 4 For more detailed information about disposal of your old appliance please contact your city office waste disposal service or visit our web site www nanoentek com This product conforms to UL 61010 1 CAN CSA C22 2 No 61010 1 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part General Require ments Instruments bearing the TUV symbol are certified by TUV Product Services to be in conformance with the applicable safety standard for the US and Canada e c N 2014 NanoEntek Inc Arthur Image based cytometer 41 Product specifications Environmental conditions Operating power Freguency Installation site Operating temperature Maximum Relative Humidity Instrument Counting time Cell measurement range Cell size range Optics Excitation Filters Camera USB drive Dimension Weight Cell analysis slide Material Dimension Chamber sample volume 100 240 VAC 2 5 A 120 W 50 60 Hz Indoor use only 5 40 C 20 95 10 sec 2 min
27. onal copyright laws They cannot be reproduced translated published or distributed without the permission of the copyright owner Arthur User manual Website www nanoentek com E mail sales nanoentek com Manufactured by NanoEnTek Inc 12F 5 Digital ro 26 gil Guro gu Seoul 152 740 Korea Website www nanoentek com E mail sales nanoentek com The information in this manual is described as accurately as possible Firmware and software changes and updates may change without prior consent or notification Copyright 2014 by NanoEnTek Inc All rights reserved Published in Korea Documentation NESMU ATT 001E Revision history V 0 0 Date SEP 2014 O 2014 NanoEntek Inc Arthur Image based cytometer 45 Arthur Image based cytometer NESMU ATT 001E V 0 0 EnTek NanoEnTek Inc HQ 5 Digital ro 26 gil Guro gu Seoul 152 740 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 NanoEnTek USA Inc 5627 Stoneridge Drive Suite 304 Pleasanton CA 94588 USA Tel 1 925 225 0108 1 888 988 0108 Toll free Fax 1 925 225 0109 Email sales nanoentek com Website www nanoentek com
28. ound measurement select the other file name from the drop down menu 3 To perform a new background measurement load the slide on the unstained cell control insert the slide into the instrument and press the Press to insert new unstained cell control button Press to insert new unstained cell control 4 Press the Press to run unstained cell control button after Arthur cell analysis slide is completely pulled into the instrument Press to run unstained cell control 2014 NanoEntek Inc Arthur Image based cytometer 23 Operation Running sample Capture 1 Select the number of images from the drop down menu to specify the number of fields of view to capture 4 fields LA 9fields 13 fields 18 fields 20 fields of images to capture 2 Press the Press to run sample button to capture the selected number of images 3 After capturing the specified number of fields of view the Arthur cell analysis slide will be ejected automatically The analysis result data including average cell size the counted number of cells total cell concentration assay specific data other specific data and histogram plots will be displayed in the data window located in the sample tab 82X 1095 cogis tL 286X 1004 cols mL 14X 1006 celmi 1 mo RES A Jak T0095 H fv f ji cal sce 16 um css counied 305 Total cell conc 8 37X 1065 cedk mi Gal
29. scence which are specific to the assay in use page 27 z The proper alignment of the two cameras inside the Arthur is required to obtain the most accurate data for rescent image with the bright field image Operation Settings 1 Load 25 uL of Arthur Alignment Beads on the Arthur Cell analysis slide page 20 Align cameras zs Make sure to mix the bead thoroughly before pipeting Load the bead gently to spread it evenly in the cell analysis slide 3 Insert the Arthur cell analysis slide containing the Arthur Alignment beads into the instrument 2 Press the Align cameras to start the alignment procedure Fame Mamata Cala Load alignment beds Focus your sample using Me focus knob HOTE FOCUS your sarnple Hsing imam knob 5 Press the Run button to start the auto alignment Run 2014 NanoEntek Inc Arthur Image based cytometer 11 Operation Settings 6 Press the OK button to confirm the correct alignment of the cameras Align cameras Press the Cancel button to cancel the alignment Hoge TEE You can also manually adjust the cameras by positioning the green circles over the corresponding Alignment beads using the directional keys on this screen but this is not recommended N IMPORTANT When installing or moving Arthur it is recommended to perform auto alignment Operation Settings Calibrating the green and red fluorescent channels o
30. stogram will have four individual thresholds which are used for partitioning the cell population into the stages of the cell cycle Each threshold can be set as described above and the color of each partition will correspond to the appropriate data set in the data table Biological molecules found within cells fluoresce upon excitation and result in background fluorescence Because the Arthur is a highly sensitive instrument it detects this background fluorescence and displays it as a peak closest to the 0 RFU value To eliminate the background fluorescence from your calculations adjust the threshold to exclude this peak 28 Operation Result Viability Result Apoptosis Home Maire Mao ure CLIQUE all O 2014 NanoEntek Inc Arthur Image based cytometer 9 Operation Result Cell cycling Result Green fluorescence Home Lain Home M ki SLM EFE E ILE s ms Ducrescerim cimi LC j Doini LT min mm um ee ell ANSE Mao durae suttings 30 Operation Data Select data file 1 Press the Data menu to see the stored data files in a list on the right side of the screen Homa ET TZ ml Nb M HE Anas m 2 Move up and down the Green square button on the scrollbar to see all data files 3 Select the data file to review by touching the corresponding line on the file list Sel
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